Carg08285-RA (mRNA) Silver-seed gourd

Overview
NameCarg08285-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionUbiquitin-conjugating enzyme E2
LocationCucurbita_argyrosperma_scaffold_015 : 631000 .. 634245 (-)
Sequence length354
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGTTAGCCACTATACTTAGTTATGTATATCATGAGTCCACACGAAGGGATCCGACTACGAGTATGTTGAGGATTGTTGGGAGTGAGTACCACGTTGGCCAATTTAGGGAACGATCATGTGTTTATAAGCAAAATACCTCTCTATTGGTATGAGGCATTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCATAGTAGACAATATCATACCATTGTGGAGAGTCATGATTCCTAACATGGCATTAGAGCCCTGCCGTAAACCTAGCTATGACAATAGGATCCTCAAATATTGAACAAAGAATTGTGAGCCTTGAAGGTGTAGTCAAAAGTGACTAAAGTGTTGAACAAAGGGTGTACTTTGTTCGAGGGCTCCAGAGAAAGGAGTCGAGTCTCGATTAAGGGGAGACTTATAGTGTATTTTGTTCGAGTTGAGGATCGTTGGGAGTGTGTCTCACATTGGCTAATTTAGAGAATGATCATGGATTTATAAGTAGAGTAATATCTCTCCATTGGTATGAAGCCTTTTGGGGAAGTCCAAAGCAAAGCTATGAGAGCTTATGCTCAAAGTGGACAATATCATATCATTGTGGAGAGTTGTGATTCCTAGTAGAGGTTCAGTCAAGTATCATGTCTGAGCCTTTGATTTTGTGTAGGCGTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAGTTGGTTTGTGACGATTCCAATATTTTTAAATGGATAGCGCTAATCAAGGTAACCCTCTCAATGATCCAGATGAAATATGTGGTTTCAGAGTTTTTCTTTTCTTTTTTTTTTTTTTTTTGCTTACCATGATTGATATGCATTTTTATTTAGGGACCATCAGAGACTCCTTTTGAAGGTGGTGTCTTCCAGCTTGCCTTATCCGTACCTGAGCAGTACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGGTAGAAGTTGCTCTGGTCCTTTTTCTAGATCCTATGCTGACTATGAGCCTTTAGAAAGAAAGAAGAATACACCAAAATGTAGGTGCTGTACCGTTGTTGTAAGTGAATGTAATGAAAAGATCGATGCGAGCCGCGAGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGATCTCAAAATAATAGGGGTTAAGGTGGGCCGGTGACACGTAGGGGATAAGCTTGATCATGGGGGAGGAAAGGGAAACACTAGGGGAGGGGGTACCGTTGTCCAAGGGAGAGTAGTGAGCAGCAGTATTTAGGTCGTTTTTTAATTAGTTAACAAATACCGTTGAGCATCTGTGCATGGAGGGGAATATGGTGGAGAATATATCAGAGTGAGATCCCAGCGTCAGCGAGGATGTTGGGCCCTAAAGGGGATGGATTGCGAGATCCCACATCGGTTGGAGAGGGCAACAAAACATTCTTTATAAGGGTGTGGAAATCTCTCCCTAGCTGACGCGTTTTAAAACCTCGAGGGGAAGTCTCGAAGGAAAAGCGCAAAGAGGACAATATCTGCTAGTTGTGAAGGTACACGGGAGTTGCAATGATGCTAGCGGTGGAGGTACCACGGGAGAAGAGTTTACGACAAGCAACCGGGTAAAAAGGAAGTGAACTCAATAGAAAGAAGCGACTGGGATTAGGAGTGGGAAGCTAGATATTTCGTGGACTGCTTTGTTCATATGAGAGGAGTTCTTGTTGCAGAGCAATTCAGTGCATTAGTGAGTTGCAGTCTAAAGGGAGAAGGAGAGGTTCGGTATGATTTTGATGGGTGGCTTGGAGCCCGAAAGAGGGTGATCCGGTCACGGGACGAGTAGAGAACCCGAATATTCCTTATTCCAACTTTTGCAGAAGGCCATACTCAGTGAAAATTCATAGCTCTCCGGCCACTGGCATATGAAGTGCTTGTGTAACGCCATTGATTGAAATAGTATAAGTTGCATTCTTTGCTATGAACTATATTGATGAATAGTGATCATTCAAGTTCGCCGACCCCTTTTGTGATTGTAGTCTTAGTTTATAGTGAACCTTATTTTTAATACACGAATCGAACCACACTTATACTTACCGATGTACGTCGTTCCTGCAGACGGGTGAGATCTGCCTAGACATATTGAAGAACGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATCGCTTTGATGGCTCATCCCGAACCTGATAGCCCGCTAAACTGTGATTCTGGTGAGTTTTCATGGATTCCTTATGATGAATTTTCCTGATTTATATGTTCATTTATTTGAATCTTTGTGATGGCGTGATAATGTAGCAAAGTAGTTAATTGATCTGAAAACTGCTTCTGGTTCGCGATTGAATTAGTTCATCATTCCTAAACCATTCTTTTGGAGAATGATTTGCATAATAGTTAAGAATAGATGCAAAAATTATGGGAAGAACACTGAGCTAGTCGCTTGAATCTATAGGCAACCTCTCCTCGATCAAACAAACTCTGAATTTCATAAGAGAATCAAGATCGTAGTTGCATCAGTTTGGCTCGGGTCTAGGCCGATACTGATGTAAACTAGCTTATCGATTTATGAATATAAAAACCCAATAAGACTAAACCAGTTGAGTTCAGTTGTATATTTTTAAATTGAATCGATTGATAATGCTCGAGATATTGGGGAATTTCACTCCTTAGGAAGGCGTTGTGCATTGTGAGCCAGCTTCAAGGAGGCAACTAGAATTCAATTTTTAATGAATACGGCTCCGTTTGATAACTATTTAGTTTTTAGTTTCTGAAAAGTGTGCTTATTTTCTCGTAATTTCTTTAGAATAATTTATCTTTCTGAAGGCAATATTTTGGTGAAAGTTGTGTTTATAAGCTAAATTTCCAAAATAAAAAATCGAAAAGGCTCGAACTTAGGTCTGGGGTGATTCAACTTAAGACCTTGGAGAGAATCTCCGAACATTCTAAGTACAACGGTATCATTTGGAGGTGAAATAATTGAAGCGTGTTGCTCGGTTTCATAAACTTTTTTTGGGATCAAACCCCGGTAACACATATAAAGCAATTCGAGTGATCTTCAATTCTTCTGACCGATTGTTTGGGTGTTTACAGGAAATCTTCTGCGTTCGGGCGATGTCCGAGGTTATCAATCAATGGCAAGGATGTACACAAGGCTGGCTGCCATGCCCAAAAAGGGCTGA

mRNA sequence

ATGCAGGGACCATCAGAGACTCCTTTTGAAGGTGGTGTCTTCCAGCTTGCCTTATCCGTACCTGAGCAGTACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGACGGGTGAGATCTGCCTAGACATATTGAAGAACGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATCGCTTTGATGGCTCATCCCGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAATCTTCTGCGTTCGGGCGATGTCCGAGGTTATCAATCAATGGCAAGGATGTACACAAGGCTGGCTGCCATGCCCAAAAAGGGCTGA

Coding sequence (CDS)

ATGCAGGGACCATCAGAGACTCCTTTTGAAGGTGGTGTCTTCCAGCTTGCCTTATCCGTACCTGAGCAGTACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGACGGGTGAGATCTGCCTAGACATATTGAAGAACGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATCGCTTTGATGGCTCATCCCGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAATCTTCTGCGTTCGGGCGATGTCCGAGGTTATCAATCAATGGCAAGGATGTACACAAGGCTGGCTGCCATGCCCAAAAAGGGCTGA

Protein sequence

MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Homology
[Close]
BLAST of Carg08285-RA vs. NCBI nr
Match: XP_022962320.1 (protein PEROXIN-4-like [Cucurbita moschata] >XP_022996597.1 protein PEROXIN-4-like [Cucurbita maxima] >XP_023545582.1 protein PEROXIN-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.7 bits (626), Expect = 7.6e-62
Identity = 115/117 (98.29%), Postives = 117/117 (100.00%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. NCBI nr
Match: XP_022857422.1 (protein PEROXIN-4-like [Olea europaea var. sylvestris])

HSP 1 Score: 245.4 bits (625), Expect = 9.9e-62
Identity = 114/117 (97.44%), Postives = 117/117 (100.00%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGD+RGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDIRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. NCBI nr
Match: PNT33458.1 (hypothetical protein POPTR_006G240900v3 [Populus trichocarpa])

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-61
Identity = 115/117 (98.29%), Postives = 115/117 (98.29%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           MQGPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 1   MQGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAA PKKG
Sbjct: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAASPKKG 117

[Close]
BLAST of Carg08285-RA vs. NCBI nr
Match: KHN18193.1 (Protein PEROXIN-4 [Glycine soja] >KRH52690.1 hypothetical protein GLYMA_06G082900 [Glycine max] >KRH52691.1 hypothetical protein GLYMA_06G082900 [Glycine max] >KRH52692.1 hypothetical protein GLYMA_06G082900 [Glycine max])

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. NCBI nr
Match: XP_015945667.1 (protein PEROXIN-4 [Arachis duranensis] >XP_015945668.1 protein PEROXIN-4 [Arachis duranensis] >XP_015945669.1 protein PEROXIN-4 [Arachis duranensis] >XP_016181130.1 protein PEROXIN-4 [Arachis ipaensis] >XP_025623803.1 protein PEROXIN-4 [Arachis hypogaea] >XP_025623804.1 protein PEROXIN-4 [Arachis hypogaea] >XP_025623805.1 protein PEROXIN-4 [Arachis hypogaea] >XP_025623806.1 protein PEROXIN-4 [Arachis hypogaea] >XP_025681396.1 protein PEROXIN-4 [Arachis hypogaea])

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. TAIR10
Match: AT5G25760.1 (peroxin4)

HSP 1 Score: 235.7 bits (600), Expect = 1.4e-62
Identity = 109/117 (93.16%), Postives = 114/117 (97.44%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETP+EGGVFQLA SVPE YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+MYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. TAIR10
Match: AT1G16890.3 (ubiquitin-conjugating enzyme 36)

HSP 1 Score: 110.9 bits (276), Expect = 5.3e-25
Identity = 53/110 (48.18%), Postives = 79/110 (71.82%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAW 62
           GP+++P+EGGVF+L L +PE+YP+  P+VRFLTKI+HPN+  K G ICLDILK+ WSPA 
Sbjct: 52  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-KLGRICLDILKDKWSPAL 111

Query: 63  TLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAA 113
            +++V  +I AL++ P PD PL+ +     +S +    ++ A+ +TRL A
Sbjct: 112 QIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKEWTRLYA 159

[Close]
BLAST of Carg08285-RA vs. TAIR10
Match: AT5G53300.1 (ubiquitin-conjugating enzyme 10)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-24
Identity = 51/113 (45.13%), Postives = 73/113 (64.60%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           + GPSE+P+ GGVF + +  P  YP +PP+V F TK+FHPN++   G ICLDILK  WSP
Sbjct: 37  IMGPSESPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNIN-SNGSICLDILKEQWSP 96

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAM 114
           A T+  V  +I +L+  P PD PL  +  ++ ++ D   Y+S AR +T+  AM
Sbjct: 97  ALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT-DKNKYESTARSWTQKYAM 147

[Close]
BLAST of Carg08285-RA vs. TAIR10
Match: AT4G27960.2 (ubiquitin conjugating enzyme 9)

HSP 1 Score: 108.2 bits (269), Expect = 3.4e-24
Identity = 50/113 (44.25%), Postives = 73/113 (64.60%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           + GPS++P+ GGVF + +  P  YP +PP+V F TK+FHPN++   G ICLDILK  WSP
Sbjct: 67  IMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNIN-SNGSICLDILKEQWSP 126

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAM 114
           A T+  V  +I +L+  P PD PL  +  ++ ++ D   Y+S AR +T+  AM
Sbjct: 127 ALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT-DKNKYESTARTWTQKYAM 177

[Close]
BLAST of Carg08285-RA vs. TAIR10
Match: AT1G64230.4 (ubiquitin-conjugating enzyme 28)

HSP 1 Score: 107.8 bits (268), Expect = 4.5e-24
Identity = 51/113 (45.13%), Postives = 73/113 (64.60%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           + GPS++P+ GGVF + +  P  YP +PP+V F TK+FHPNV+   G ICLDILK  WSP
Sbjct: 79  IMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVN-SNGSICLDILKEQWSP 138

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAM 114
           A T+  V  +I +L+  P PD PL  +  ++ ++ D   Y+S AR +T+  AM
Sbjct: 139 ALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKT-DRAKYESTARSWTQKYAM 189

[Close]
BLAST of Carg08285-RA vs. Swiss-Prot
Match: sp|Q8LGF7|PEX4_ARATH (Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 2.6e-61
Identity = 109/117 (93.16%), Postives = 114/117 (97.44%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETP+EGGVFQLA SVPE YPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+MYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. Swiss-Prot
Match: sp|Q86IZ3|UBCX_DICDI (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex4 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.4e-43
Identity = 75/110 (68.18%), Postives = 97/110 (88.18%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GP +TP+E G F+L +SVP  YPLQPP ++F+TKIFHPN+HFKTGEICLD+LK +WS 
Sbjct: 37  IKGPPDTPYENGKFELDISVPTNYPLQPPTIKFVTKIFHPNIHFKTGEICLDLLKTSWSA 96

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRL 111
            +TLQSVCR+IIAL++ PE DSPLNCD+GNL+R+GDV+G+ S+ARMYTRL
Sbjct: 97  IYTLQSVCRSIIALLSLPEADSPLNCDAGNLIRNGDVKGHDSLARMYTRL 146

[Close]
BLAST of Carg08285-RA vs. Swiss-Prot
Match: sp|Q9P6I1|UBC16_SCHPO (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc16 PE=3 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 3.1e-30
Identity = 60/115 (52.17%), Postives = 80/115 (69.57%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GP+ETP+EGG + L + V E YP+ PP V F TKI HPN+ +  GE+C+DILK  WSP
Sbjct: 41  IEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPK 116
           AW+LQS C AII+L+++ +  SPLN D+  LLR+GD   Y S+ R  T L A  K
Sbjct: 101 AWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRCTTYLYAKGK 155

[Close]
BLAST of Carg08285-RA vs. Swiss-Prot
Match: sp|O60015|UBCX_PICAN (Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 5.4e-27
Identity = 56/129 (43.41%), Postives = 78/129 (60.47%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLT----------------------KIF 60
           ++GP ++PFE  +++L + +P  YPL PP+++F+                       K+ 
Sbjct: 48  IRGPDQSPFEDALWKLEIDIPTNYPLDPPKIKFVVFGEEKIRQLQRKTSSGARKVCYKMP 107

Query: 61  HPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVR 108
           HPNV+FKTGEICLDIL+  WSPAWTLQS   AI+ L+A+PEP SPLN D  NLL+  D  
Sbjct: 108 HPNVNFKTGEICLDILQQKWSPAWTLQSALVAIVVLLANPEPLSPLNIDMANLLKCDDTT 167

[Close]
BLAST of Carg08285-RA vs. Swiss-Prot
Match: sp|Q9EQX9|UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 5.0e-25
Identity = 56/111 (50.45%), Postives = 78/111 (70.27%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAW 62
           GP ++PFEGG F+L L +PE+YP+  P+VRF+TKI+HPNV  K G ICLDILK+ WSPA 
Sbjct: 41  GPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVD-KLGRICLDILKDKWSPAL 100

Query: 63  TLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAM 114
            +++V  +I AL++ P PD PL  D     +S + +  ++ AR +TRL AM
Sbjct: 101 QIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIET-ARAWTRLYAM 149

[Close]
BLAST of Carg08285-RA vs. TrEMBL
Match: tr|A0A2K2A7E4|A0A2K2A7E4_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G240900v3 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 8.5e-62
Identity = 115/117 (98.29%), Postives = 115/117 (98.29%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           MQGPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 1   MQGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAA PKKG
Sbjct: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAASPKKG 117

[Close]
BLAST of Carg08285-RA vs. TrEMBL
Match: tr|I1K9A6|I1K9A6_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100305756 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. TrEMBL
Match: tr|I3SC14|I3SC14_LOTJA (Uncharacterized protein OS=Lotus japonicus OX=34305 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. TrEMBL
Match: tr|A0A1J7H810|A0A1J7H810_LUPAN (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_24215 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

[Close]
BLAST of Carg08285-RA vs. TrEMBL
Match: tr|A0A0B2QDV8|A0A0B2QDV8_GLYSO (Protein PEROXIN-4 OS=Glycine soja OX=3848 GN=glysoja_025083 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-61
Identity = 114/117 (97.44%), Postives = 116/117 (99.15%), Query Frame = 0 

Query: 1   MQGPSETPFEGGVFQLALSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 60
           ++GPSETPFEGGVFQLA SVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP
Sbjct: 41  IKGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSP 100

Query: 61  AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 118
           AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Sbjct: 101 AWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG 157

The following BLAST results are available for this feature:
BLAST of Carg08285-RA vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022962320.17.6e-6298.29protein PEROXIN-4-like [Cucurbita moschata] >XP_022996597.1 protein PEROXIN-4-li... [more]
XP_022857422.19.9e-6297.44protein PEROXIN-4-like [Olea europaea var. sylvestris][more]
PNT33458.11.3e-6198.29hypothetical protein POPTR_006G240900v3 [Populus trichocarpa][more]
KHN18193.12.2e-6197.44Protein PEROXIN-4 [Glycine soja] >KRH52690.1 hypothetical protein GLYMA_06G08290... [more]
XP_015945667.12.2e-6197.44protein PEROXIN-4 [Arachis duranensis] >XP_015945668.1 protein PEROXIN-4 [Arachi... [more]
BLAST of Carg08285-RA vs. TAIR10
Match NameE-valueIdentityDescription
AT5G25760.11.4e-6293.16peroxin4[more]
AT1G16890.35.3e-2548.18ubiquitin-conjugating enzyme 36[more]
AT5G53300.12.0e-2445.13ubiquitin-conjugating enzyme 10[more]
AT4G27960.23.4e-2444.25ubiquitin conjugating enzyme 9[more]
AT1G64230.44.5e-2445.13ubiquitin-conjugating enzyme 28[more]
BLAST of Carg08285-RA vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8LGF7|PEX4_ARATH2.6e-6193.16Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1[more]
sp|Q86IZ3|UBCX_DICDI1.4e-4368.18Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex... [more]
sp|Q9P6I1|UBC16_SCHPO3.1e-3052.17Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
sp|O60015|UBCX_PICAN5.4e-2743.41Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 ... [more]
sp|Q9EQX9|UBE2N_RAT5.0e-2550.45Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV... [more]
BLAST of Carg08285-RA vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A2K2A7E4|A0A2K2A7E4_POPTR8.5e-6298.29Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G240900v3 PE=... [more]
tr|I1K9A6|I1K9A6_SOYBN1.5e-6197.44Uncharacterized protein OS=Glycine max OX=3847 GN=100305756 PE=3 SV=1[more]
tr|I3SC14|I3SC14_LOTJA1.5e-6197.44Uncharacterized protein OS=Lotus japonicus OX=34305 PE=2 SV=1[more]
tr|A0A1J7H810|A0A1J7H810_LUPAN1.5e-6197.44Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_24215 PE=3 S... [more]
tr|A0A0B2QDV8|A0A0B2QDV8_GLYSO1.5e-6197.44Protein PEROXIN-4 OS=Glycine soja OX=3848 GN=glysoja_025083 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR023313UBQ-conjugating_AS
IPR016135UBQ-conjugating_enzyme/RWD
IPR000608UBQ-conjugat_E2
GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg08285Carg08285gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg08285-RACarg08285-RA-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08285-RA.CDS.4Carg08285-RA.CDS.4CDS
Carg08285-RA.CDS.3Carg08285-RA.CDS.3CDS
Carg08285-RA.CDS.2Carg08285-RA.CDS.2CDS
Carg08285-RA.CDS.1Carg08285-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg08285-RA.exon.4Carg08285-RA.exon.4exon
Carg08285-RA.exon.3Carg08285-RA.exon.3exon
Carg08285-RA.exon.2Carg08285-RA.exon.2exon
Carg08285-RA.exon.1Carg08285-RA.exon.1exon


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 1..113
e-value: 3.8E-29
score: 112.8
NoneNo IPR availablePANTHERPTHR43902:SF1UBIQUITIN-CONJUGATING ENZYME E2-21 KDAcoord: 1..116
NoneNo IPR availablePANTHERPTHR43902FAMILY NOT NAMEDcoord: 1..116
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 1..107
e-value: 8.4E-39
score: 132.3
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBIQUITIN_CONJUGAT_2coord: 1..93
score: 32.16
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 3..108
e-value: 5.67684E-42
score: 136.178
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 1..113
e-value: 5.7E-46
score: 157.9
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILYSSF54495UBC-likecoord: 3..113
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBIQUITIN_CONJUGAT_1coord: 38..54