Carg06923-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg06923-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Lipoxygenase
Location	Cucurbita_argyrosperma_scaffold_065 : 331201 .. 332255 (-)
Sequence length	444

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCCGGCGAACCCAGGGAAGAAAGACAAGCTGGGATCAAAGCCTTTCTCGGATTTCACTCAGATTGACCTAATGTTCCGAGATGGCATTGAGATCCCCCCCGCCCCTCACGAAATTCTTCGCTTTAATATTTCCTCCACTCTTTCCACCATGCATGCCCCACCCTTTGTTGACCCTTCTACCCTTCAGCCCCAGCCTGCTCGATTGGTTAAATTCCCACCACCCGAGGCTCTCAAGAGTGAGTCCACGCCTACTTTTCTTTTTTTTTTTTTGGGTTGGGGAGGGGGGGGTGTGTATTAATGGAGCTTACCAAACAAATGCATTTTCCTCACAATTACTTCAATTTTTCCCAGGAGACAAGTTCAATTGGCTGAGTGACAGCGAGTTTGCAAGACAGACACTCGCTGGTCTCAACCCCTACAGCATTCAGCTGGTCAAGGTCACTCACAACTTCACCATCAATCCATTAATCTCTCTCTTTCTATAATATGCAAAACTCGCTTAATTTTGTCCTCATACATTCAGAGTTTGCCTTTGATGAGTGAGCTGAACCCCAAGGATTATGGACCTCAAGAATCAGGATTCACCACCGAATTGGTTCAAAAATTAATCGGATGCTCCATCACTGTTGAAGAGGTAAAATTGATTAAACCCAACGTGCTATAAGTTACTAAATTAGAGAAGAAAAAAAATAAAAAAATAAAAAATAAAAAAAGAGAATTTTGTGAGTTTATGCAGGCATTAGCACACAAGAAATTGTTCGTACTGGATTACCATGACACTTTAATGCAATATGTAACCAAAGTAAGAAGCATTAAGCGGACGACACTGTATGGATCAAGGACATTGTTCTTCCTAAACTCAGATGACACTTTGACGCCGTTGGGTATTGAGCTAACTCGGCCGCCAATGGATGGGAAGCCCCAATGGAAACAAGTTTTTTCCCCAAGCACTCATGCCACTGATGCCTGGCTATGGAGGCTCGCTAAAGCCCACGTTCTTGCTCATGATTCTTGCGTTCACCAACTCGTTATTCATTGGTACAGGGCTAA

mRNA sequence

ATGTTTCCGGCGAACCCAGGGAAGAAAGACAAGCTGGGATCAAAGCCTTTCTCGGATTTCACTCAGATTGACCTAATGTTCCGAGATGGCATTGAGATCCCCCCCGCCCCTCACGAAATTCTTCGCTTTAATATTTCCTCCACTCTTTCCACCATGCATGCCCCACCCTTTGTTGACCCTTCTACCCTTCAGCCCCAGCCTGCTCGATTGGTTAAATTCCCACCACCCGAGGCTCTCAAGAGAGACAAGTTCAATTGGCTGAGTGACAGCGAGTTTGCAAGACAGACACTCGCTGGTCTCAACCCCTACAGCATTCAGCTGGTCAAGAGTTTGCCTTTGATGAGTGAGCTGAACCCCAAGGATTATGGACCTCAAGAATCAGGATTCACCACCGAATTGGTTCAAAAATTAATCGGATGCTCCATCACTGTTGAAGAGGGCTAA

Coding sequence (CDS)

ATGTTTCCGGCGAACCCAGGGAAGAAAGACAAGCTGGGATCAAAGCCTTTCTCGGATTTCACTCAGATTGACCTAATGTTCCGAGATGGCATTGAGATCCCCCCCGCCCCTCACGAAATTCTTCGCTTTAATATTTCCTCCACTCTTTCCACCATGCATGCCCCACCCTTTGTTGACCCTTCTACCCTTCAGCCCCAGCCTGCTCGATTGGTTAAATTCCCACCACCCGAGGCTCTCAAGAGAGACAAGTTCAATTGGCTGAGTGACAGCGAGTTTGCAAGACAGACACTCGCTGGTCTCAACCCCTACAGCATTCAGCTGGTCAAGAGTTTGCCTTTGATGAGTGAGCTGAACCCCAAGGATTATGGACCTCAAGAATCAGGATTCACCACCGAATTGGTTCAAAAATTAATCGGATGCTCCATCACTGTTGAAGAGGGCTAA

Protein sequence

MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTELVQKLIGCSITVEEG

Homology

[Close]

BLAST of Carg06923-RA vs. NCBI nr
Match: XP_022943470.1 (lipoxygenase 7, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 302.4 bits (773), Expect = 8.6e-79
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 60
           MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP
Sbjct: 254 MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 313

Query: 61  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 120
           STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK
Sbjct: 314 STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 373

Query: 121 DYGPQESGFTTELVQKLIGCSITVEE 147
           DYGPQESGFTTELVQKLIGCSITVEE
Sbjct: 374 DYGPQESGFTTELVQKLIGCSITVEE 399

[Close]

BLAST of Carg06923-RA vs. NCBI nr
Match: XP_022943469.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 302.4 bits (773), Expect = 8.6e-79
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 60
           MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP
Sbjct: 254 MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 313

Query: 61  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 120
           STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK
Sbjct: 314 STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 373

Query: 121 DYGPQESGFTTELVQKLIGCSITVEE 147
           DYGPQESGFTTELVQKLIGCSITVEE
Sbjct: 374 DYGPQESGFTTELVQKLIGCSITVEE 399

[Close]

BLAST of Carg06923-RA vs. NCBI nr
Match: XP_022985897.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 301.2 bits (770), Expect = 1.9e-78
Identity = 145/146 (99.32%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 60
           MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP
Sbjct: 250 MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 309

Query: 61  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 120
           STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK
Sbjct: 310 STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 369

Query: 121 DYGPQESGFTTELVQKLIGCSITVEE 147
           DYGPQESGFTT+LVQKLIGCSITVEE
Sbjct: 370 DYGPQESGFTTQLVQKLIGCSITVEE 395

[Close]

BLAST of Carg06923-RA vs. NCBI nr
Match: XP_023513406.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.9 bits (764), Expect = 9.5e-78
Identity = 144/146 (98.63%), Postives = 145/146 (99.32%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 60
           MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP
Sbjct: 254 MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 313

Query: 61  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 120
           ST+QPQPARLVKFPPPEALKRDKFNWLSD EFARQTLAGLNPYSIQLVKSLPLMSELNPK
Sbjct: 314 STVQPQPARLVKFPPPEALKRDKFNWLSDIEFARQTLAGLNPYSIQLVKSLPLMSELNPK 373

Query: 121 DYGPQESGFTTELVQKLIGCSITVEE 147
           DYGPQESGFTTELVQKLIGCSITVEE
Sbjct: 374 DYGPQESGFTTELVQKLIGCSITVEE 399

[Close]

BLAST of Carg06923-RA vs. NCBI nr
Match: KGN54123.1 (hypothetical protein Csa_4G286960 [Cucumis sativus])

HSP 1 Score: 225.7 bits (574), Expect = 1.0e-55
Identity = 114/148 (77.03%), Postives = 124/148 (83.78%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTL--STMHAPPFV 60
           MFP+NPGKKDKLG+KPF+DFTQIDLMFRDGI  P  PHEIL+FNI STL  S   APP V
Sbjct: 164 MFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPPV 223

Query: 61  DPSTLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELN 120
            P  +QP    ++KFPPPEALKRDKFNWLSDSEFARQT+AGLNPY IQLVKS PL SEL+
Sbjct: 224 QPIPVQPN-TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELD 283

Query: 121 PKDYGPQESGFTTELVQKLIGCSITVEE 147
           P +YGPQES FTTELVQKLIG SITVEE
Sbjct: 284 PVEYGPQESAFTTELVQKLIGSSITVEE 310

[Close]

BLAST of Carg06923-RA vs. TAIR10
Match: AT1G17420.1 (lipoxygenase 3)

HSP 1 Score: 91.3 bits (225), Expect = 5.4e-19
Identity = 49/138 (35.51%), Postives = 77/138 (55.80%), Query Frame = 0


Query: 6   PGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQP 65
           P  K  + ++ F+DF +ID ++++G+        +L+      +      P V   TLQ 
Sbjct: 347 PSLKASIVAEDFADFGEIDRLYKEGL--------LLKLGFQDDIFKKFPLPKVVVDTLQE 406

Query: 66  QPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQ 125
               L+K+  P+ L +DK  WL D EFARQ +AG+NP +I+ VK+ P +S L+PK YGPQ
Sbjct: 407 STKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQ 466

Query: 126 ESGFTTE-LVQKLIGCSI 143
            S  T + ++  L G S+
Sbjct: 467 HSALTDDHIIGHLDGFSV 476

[Close]

BLAST of Carg06923-RA vs. TAIR10
Match: AT3G45140.1 (lipoxygenase 2)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-18
Identity = 50/132 (37.88%), Postives = 76/132 (57.58%), Query Frame = 0


Query: 15  KPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQPQPARLVKFP 74
           +PF  F  I  +F +GI++P                 +   P +  +  + Q   +++F 
Sbjct: 336 EPFPHFKAIQNLFEEGIQLP------------KDAGLLPLLPRIIKALGEAQD-DILQFD 395

Query: 75  PPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTELV 134
            P  + RD+F+WL D EFARQTLAGLNPYSIQLV+  PL+S+L+P  YG   S  T E+V
Sbjct: 396 APVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIV 454

Query: 135 QKLIGCSITVEE 147
           ++ +  ++TV+E
Sbjct: 456 EREVKGNMTVDE 454

[Close]

BLAST of Carg06923-RA vs. TAIR10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 78.2 bits (191), Expect = 4.8e-15
Identity = 34/67 (50.75%), Postives = 48/67 (71.64%), Query Frame = 0


Query: 70  LVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGF 129
           L+K+  P  +K D+F WL D+EF RQ LAG+NP +I+L+K LP+ S L+P  YGPQES  
Sbjct: 397 LLKYDTPAVIKWDRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVL 456

Query: 130 TTELVQK 137
           T E++ +
Sbjct: 457 TEEIIAR 463

[Close]

BLAST of Carg06923-RA vs. TAIR10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 76.6 bits (187), Expect = 1.4e-14
Identity = 42/139 (30.22%), Postives = 77/139 (55.40%), Query Frame = 0


Query: 6   PGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQP 65
           P  K  + ++ F++F +ID ++++G+        +L+      +      P +  +TLQ 
Sbjct: 353 PSLKASILAEDFANFGEIDSLYKEGL--------LLKLGFQDDMFKKFPLPKI-VTTLQK 412

Query: 66  QPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQ 125
               L+++  P+ + +DK+ WL D EFARQ +AG+NP +I+ V S P +S L+P+ YGP 
Sbjct: 413 SSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPG 472

Query: 126 ESGFTTE--LVQKLIGCSI 143
                TE  ++ +L G ++
Sbjct: 473 LHSALTEDHIIGQLDGLTV 482

[Close]

BLAST of Carg06923-RA vs. TAIR10
Match: AT1G55020.1 (lipoxygenase 1)

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-13
Identity = 44/132 (33.33%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 15  KPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQPQPARLVKFP 74
           K F  F  +  ++ +GI++   P++ L  +I   +     P  +     +    + +KFP
Sbjct: 297 KEFDSFEDVLKIYEEGIDL---PNQALIDSIVKNI-----PLEMLKEIFRTDGQKFLKFP 356

Query: 75  PPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTELV 134
            P+ +K DK  W +D EFAR+ LAGLNP  IQL+K  P  S+L+ + YG Q S  T   +
Sbjct: 357 VPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHI 416

Query: 135 QKLIGCSITVEE 147
           +  +   +TVEE
Sbjct: 417 EHNLD-GLTVEE 419

[Close]

BLAST of Carg06923-RA vs. Swiss-Prot
Match: sp|P38419|LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 2.8e-20
Identity = 53/132 (40.15%), Postives = 83/132 (62.88%), Query Frame = 0


Query: 16  PFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQPQPA-RLVKFP 75
           PF  F  ID +F DG+E+P     + + N   ++     P  ++   L+  PA ++++F 
Sbjct: 360 PFPSFFVIDKLFEDGVELP----GVDKLNFLESV----VPRLLE--HLRDTPAEKILRFE 419

Query: 76  PPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTELV 135
            P  +++DKF WL D EFAR+TLAG+NPY+I+LV+  PL S+L+P  YGP ES  T +L+
Sbjct: 420 TPANIQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLL 479

Query: 136 QKLIGCSITVEE 147
           ++ +   +TVEE
Sbjct: 480 EEQMRRVMTVEE 481

[Close]

BLAST of Carg06923-RA vs. Swiss-Prot
Match: sp|Q8GSM2|LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 3.6e-20
Identity = 52/133 (39.10%), Postives = 79/133 (59.40%), Query Frame = 0


Query: 14  SKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQPQPARLVKF 73
           S  FS F  ID ++ DGI +P   H    FN+ + +     P  V    ++     +++F
Sbjct: 330 SHGFSHFPAIDALYSDGIPLPVDGHGGNSFNVINDV----IPRVV--QMIEDTTEHVLRF 389

Query: 74  PPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTEL 133
             PE L+RD+F+W  D EFARQTLAGLNP  I+ +   P++S+L+P  YGP ES  + E+
Sbjct: 390 EVPEMLERDRFSWFRDEEFARQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEI 449

Query: 134 VQKLIGCSITVEE 147
           ++K++   +TVEE
Sbjct: 450 LEKMMNGRMTVEE 456

[Close]

BLAST of Carg06923-RA vs. Swiss-Prot
Match: sp|O24370|LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-19
Identity = 55/131 (41.98%), Postives = 75/131 (57.25%), Query Frame = 0


Query: 17  FSDFTQIDLMFRDGIEIPP-APHEILRFNISSTLSTMHAPPFVDPSTLQPQPARLVKFPP 76
           F  F  ID +F  G+++P     +   FN+   L             +      ++ F  
Sbjct: 336 FPHFPAIDSLFNVGVDLPGLGDKKSGLFNVVPRLI----------KAISDTRKDVLLFES 395

Query: 77  PEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTELVQ 136
           P+ ++RDKF+W  D EFARQTLAGLNPYSI+LV   PL S+L+PK YGP ES  T EL++
Sbjct: 396 PQLVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIE 455

Query: 137 KLIGCSITVEE 147
           K IG  +TVE+
Sbjct: 456 KEIGNYMTVEQ 456

[Close]

BLAST of Carg06923-RA vs. Swiss-Prot
Match: sp|Q84YK8|LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.8e-19
Identity = 53/134 (39.55%), Postives = 80/134 (59.70%), Query Frame = 0


Query: 16  PFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHA--PPFVDPSTLQPQPA-RLVK 75
           PF  F  ID +F DG+E+P              L  +H+  P  ++   L+  P  +++ 
Sbjct: 377 PFPSFFVIDKLFEDGVELPGV----------EKLGFLHSIVPRLLE--LLRDSPGDKILL 436

Query: 76  FPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQESGFTTE 135
           F  P  +++DKF WL D EFAR+TLAG+NPY+I+LV+  PL S+L+P  YGP ES  T +
Sbjct: 437 FDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITAD 496

Query: 136 LVQKLIGCSITVEE 147
           L+++ +   +TVEE
Sbjct: 497 LLEEQMRRVMTVEE 498

[Close]

BLAST of Carg06923-RA vs. Swiss-Prot
Match: sp|Q9LNR3|LOX3_ARATH (Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX3 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.8e-18
Identity = 49/138 (35.51%), Postives = 77/138 (55.80%), Query Frame = 0


Query: 6   PGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDPSTLQP 65
           P  K  + ++ F+DF +ID ++++G+        +L+      +      P V   TLQ 
Sbjct: 347 PSLKASIVAEDFADFGEIDRLYKEGL--------LLKLGFQDDIFKKFPLPKVVVDTLQE 406

Query: 66  QPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPKDYGPQ 125
               L+K+  P+ L +DK  WL D EFARQ +AG+NP +I+ VK+ P +S L+PK YGPQ
Sbjct: 407 STKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQ 466

Query: 126 ESGFTTE-LVQKLIGCSI 143
            S  T + ++  L G S+
Sbjct: 467 HSALTDDHIIGHLDGFSV 476

[Close]

BLAST of Carg06923-RA vs. TrEMBL
Match: tr|A0A0A0KX68|A0A0A0KX68_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G286960 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.7e-56
Identity = 114/148 (77.03%), Postives = 124/148 (83.78%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTL--STMHAPPFV 60
           MFP+NPGKKDKLG+KPF+DFTQIDLMFRDGI  P  PHEIL+FNI STL  S   APP V
Sbjct: 164 MFPSNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPAPPPV 223

Query: 61  DPSTLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELN 120
            P  +QP    ++KFPPPEALKRDKFNWLSDSEFARQT+AGLNPY IQLVKS PL SEL+
Sbjct: 224 QPIPVQPN-TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWPLKSELD 283

Query: 121 PKDYGPQESGFTTELVQKLIGCSITVEE 147
           P +YGPQES FTTELVQKLIG SITVEE
Sbjct: 284 PVEYGPQESAFTTELVQKLIGSSITVEE 310

[Close]

BLAST of Carg06923-RA vs. TrEMBL
Match: tr|A0A1S4DXN5|A0A1S4DXN5_CUCME (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC107991008 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.2e-54
Identity = 108/146 (73.97%), Postives = 123/146 (84.25%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 60
           MFP NPGKKDKLG+KPF+DFTQIDLMFRDGI  P  PHEIL+FNI STL+  + P  V P
Sbjct: 1   MFPTNPGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEILQFNIISTLNASNQPS-VQP 60

Query: 61  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 120
           + +QP    ++KFPPPEALKRDKFNWLSD EFARQT+AGLNPY IQLVKS PL SEL+P+
Sbjct: 61  TPVQPD-TPVIKFPPPEALKRDKFNWLSDGEFARQTIAGLNPYCIQLVKSWPLKSELDPE 120

Query: 121 DYGPQESGFTTELVQKLIGCSITVEE 147
           +YGPQES FTT+LVQKLIG S+TVEE
Sbjct: 121 EYGPQESAFTTDLVQKLIGSSLTVEE 144

[Close]

BLAST of Carg06923-RA vs. TrEMBL
Match: tr|A0A0A0L1U9|A0A0A0L1U9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G286940 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.3e-38
Identity = 82/112 (73.21%), Postives = 92/112 (82.14%), Query Frame = 0


Query: 1   MFPANPGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTL--STMHAPPFV 60
           MFP+N G KDKLG+KPF+DFTQIDLMFRDGI +P  PHEIL+FNI STL  S   APP V
Sbjct: 144 MFPSNSGNKDKLGTKPFTDFTQIDLMFRDGIALPSNPHEILQFNILSTLNASNQPAPPPV 203

Query: 61  DPSTLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKS 111
            P  +QP    ++KFPPPEALKRDKFNWLSDSEFARQT+AGLNPY IQLVK+
Sbjct: 204 QPIPVQPN-TPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKT 254

[Close]

BLAST of Carg06923-RA vs. TrEMBL
Match: tr|A0A1S3BNQ1|A0A1S3BNQ1_CUCME (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491544 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.7e-36
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 2   FPAN-PGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 61
           FP++ P  KD LG   F D   I+ MFR+GI+ P APH++L+FN+S+ ++  H P     
Sbjct: 245 FPSSEPDNKDLLGKNSFHDLPHIESMFREGIKAPDAPHKLLKFNLSTIVTPDHKPDLHSN 304

Query: 62  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 121
           S++   P+ L+ +PPPE+ +RD+++WLSD+EFARQTLAGLNPYSIQLV  LPLMSEL+P+
Sbjct: 305 SSV---PSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPE 364

Query: 122 DYGPQESGFTTELVQKLIGCSITVEE 147
            YGPQES F    VQ+L+GC   V E
Sbjct: 365 IYGPQESAFNHTKVQELLGCFNEVNE 387

[Close]

BLAST of Carg06923-RA vs. TrEMBL
Match: tr|A0A0A0L1V5|A0A0A0L1V5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G287530 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.7e-36
Identity = 80/146 (54.79%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 2   FPAN-PGKKDKLGSKPFSDFTQIDLMFRDGIEIPPAPHEILRFNISSTLSTMHAPPFVDP 61
           FP++ P  KD LG   FSD   I+ MFR+GI+ P APH++L FN+S+ ++     P +  
Sbjct: 155 FPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTP--DKPDLHS 214

Query: 62  STLQPQPARLVKFPPPEALKRDKFNWLSDSEFARQTLAGLNPYSIQLVKSLPLMSELNPK 121
           ++    P+ L+ +PPPE+ +RD+++WLSD+EFARQTLAGLNPYSIQLV  LPLMSEL+P 
Sbjct: 215 NSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPD 274

Query: 122 DYGPQESGFTTELVQKLIGCSITVEE 147
            YGPQES F    VQ+LIGCSI V E
Sbjct: 275 TYGPQESAFNHTKVQELIGCSIEVNE 298

The following BLAST results are available for this feature:

BLAST of Carg06923-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943470.1	8.6e-79	100.00	lipoxygenase 7, chloroplastic-like isoform X2 [Cucurbita moschata]	[more]
XP_022943469.1	8.6e-79	100.00	linoleate 13S-lipoxygenase 2-1, chloroplastic-like isoform X1 [Cucurbita moschat...	[more]
XP_022985897.1	1.9e-78	99.32	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima]	[more]
XP_023513406.1	9.5e-78	98.63	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
KGN54123.1	1.0e-55	77.03	hypothetical protein Csa_4G286960 [Cucumis sativus]	[more]

BLAST of Carg06923-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17420.1	5.4e-19	35.51	lipoxygenase 3	[more]
AT3G45140.1	2.7e-18	37.88	lipoxygenase 2	[more]
AT1G67560.1	4.8e-15	50.75	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G72520.1	1.4e-14	30.22	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G55020.1	1.2e-13	33.33	lipoxygenase 1	[more]

BLAST of Carg06923-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P38419\|LOXC1_ORYSJ	2.8e-20	40.15	Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX...	[more]
sp\|Q8GSM2\|LOX23_HORVU	3.6e-20	39.10	Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1	[more]
sp\|O24370\|LOX21_SOLTU	1.0e-19	41.98	Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO...	[more]
sp\|Q84YK8\|LOXC2_ORYSJ	1.8e-19	39.55	Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q9LNR3\|LOX3_ARATH	9.8e-18	35.51	Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX3 PE=2 SV=1	[more]

BLAST of Carg06923-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KX68\|A0A0A0KX68_CUCSA	6.7e-56	77.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G286960 PE=4 SV=1	[more]
tr\|A0A1S4DXN5\|A0A1S4DXN5_CUCME	2.2e-54	73.97	Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC107991008 PE=3 SV=1	[more]
tr\|A0A0A0L1U9\|A0A0A0L1U9_CUCSA	8.3e-38	73.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G286940 PE=4 SV=1	[more]
tr\|A0A1S3BNQ1\|A0A1S3BNQ1_CUCME	2.7e-36	53.42	Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491544 PE=3 SV=1	[more]
tr\|A0A0A0L1V5\|A0A0A0L1V5_CUCSA	2.7e-36	54.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G287530 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR036226	LipOase_C_sf
IPR000907	LipOase
IPR013819	LipOase_C
IPR027433	Lipoxygenase_dom_3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg06923	Carg06923	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg06923-RA	Carg06923-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg06923-RA.CDS.4	Carg06923-RA.CDS.4	CDS
Carg06923-RA.CDS.3	Carg06923-RA.CDS.3	CDS
Carg06923-RA.CDS.2	Carg06923-RA.CDS.2	CDS
Carg06923-RA.CDS.1	Carg06923-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg06923-RA.exon.4	Carg06923-RA.exon.4	exon
Carg06923-RA.exon.3	Carg06923-RA.exon.3	exon
Carg06923-RA.exon.2	Carg06923-RA.exon.2	exon
Carg06923-RA.exon.1	Carg06923-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027433	Lipoxygenase, domain 3	GENE3D	G3DSA:4.10.372.10		coord: 8..102 e-value: 6.4E-17 score: 63.7
IPR013819	Lipoxygenase, C-terminal	PFAM	PF00305	Lipoxygenase	coord: 14..146 e-value: 4.9E-26 score: 91.2
IPR013819	Lipoxygenase, C-terminal	PROSITE	PS51393	LIPOXYGENASE_3	coord: 1..147 score: 26.855
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 16..146
None	No IPR available	PANTHER	PTHR11771:SF65	SUBFAMILY NOT NAMED	coord: 16..146
IPR036226	Lipoxigenase, C-terminal domain superfamily	SUPERFAMILY	SSF48484	Lipoxigenase	coord: 14..146