Carg02918-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg02918-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Aspartate-tRNA ligase
Location	Cucurbita_argyrosperma_scaffold_008 : 800015 .. 800944 (+)
Sequence length	668

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTGCCATGATAACCCAACTTACAGTAACTCATTTGATGTCTTTATTCGAGGTATACATTAAATATTGTTTCATCACTATAAGTGTCTTGCTGGTGCTTATTCTTCTCTATCATTTTATGTAACAAAATTTTCTAATATATGATCCATGATAAGTAACTTAAAACTTTGTTGTTCCCGCAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTTCACGTGCCAGAATTTCTTGCAGAACGCGCTCAAGCATGTGGGATCGATGTAAAAACCATAGAAACTTACATCGATTCGTTTAGGTATGTTTCTCTTATGCTTTTGTTTTCTCTTCCCCTTTACTCGAAGTTGAAATTTAACCGAATAAACGAAATTTAACTGTGATGGCAACGTCTTGATGGGGTTTTCCTTTACGTGCAATGCTTTCATTGCAGGTATGGCGCTCCCCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTAGTGATGCTTTTTTGTGGTTTGAATAACATTCGGAAAACATCATTGTTCCCTCGCGACCCACAGAGGATTGCTCCTTAGAATTGCACCAGTAATAATGTAATTCCAGTTTTTGAAATTGACATTTTGTTGCCTCTTTCGCACTTCATAGGGAAAATATGAATTTCAGATCTCTGCAGGATGGTGTATTGAATACAATTGTTTGCAACGTTATAATGCAAAGTTTGTTTTGACCAGCGTCATTTGATTTAGATTTTTATTACAGTTTTGCCACACTTGTTTGGTTGAAGAAGGATGGTAGAACTCTTCAATAATGACGACTGTTCTCCTGTGATGGTGTGTGGAAGGAGAGATTAGAGAGGCGGCCTAACCTAGGATAACCGGTCAAACCTACCGATAGCTCTGTTATCTTTTTTAAACTTTTCTTTTCGAGCATATTCTTTAAACTTTTC

mRNA sequence

ATGCCTTGCCATGATAACCCAACTTACAGTAACTCATTTGATGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTTCACGTGCCAGAATTTCTTGCAGAACGCGCTCAAGCATGTGGGATCGATGTAAAAACCATAGAAACTTACATCGATTCGTTTAGGTATGGCGCTCCCCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTAGTGATGCTTTTTTGTGGTTTGAATAACATTCGGAAAACATCATTGTTCCCTCGCGACCCACAGAGGATTGCTCCTTAGAATTGCACCAGTAATAATGTAATTCCAGTTTTTGAAATTGACATTTTGTTGCCTCTTTCGCACTTCATAGGGAAAATATGAATTTCAGATCTCTGCAGGATGGTGTATTGAATACAATTGTTTGCAACGTTATAATGCAAAGTTTGTTTTGACCAGCGTCATTTGATTTAGATTTTTATTACAGTTTTGCCACACTTGTTTGGTTGAAGAAGGATGGTAGAACTCTTCAATAATGACGACTGTTCTCCTGTGATGGTGTGTGGAAGGAGAGATTAGAGAGGCGGCCTAACCTAGGATAACCGGTCAAACCTACCGATAGCTCTGTTATCTTTTTTAAACTTTTCTTTTCGAGCATATTCTTTAAACTTTTC

Coding sequence (CDS)

ATGCCTTGCCATGATAACCCAACTTACAGTAACTCATTTGATGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTTCACGTGCCAGAATTTCTTGCAGAACGCGCTCAAGCATGTGGGATCGATGTAAAAACCATAGAAACTTACATCGATTCGTTTAGGTATGGCGCTCCCCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTAGTGATGCTTTTTTGTGGTTTGAATAACATTCGGAAAACATCATTGTTCCCTCGCGACCCACAGAGGATTGCTCCTTAG

Protein sequence

MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP

Homology

[Close]

BLAST of Carg02918-RA vs. NCBI nr
Match: XP_022976148.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 214.2 bits (544), Expect = 2.0e-52
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA
Sbjct: 448 MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 507

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 508 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 545

[Close]

BLAST of Carg02918-RA vs. NCBI nr
Match: XP_023535979.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.2 bits (544), Expect = 2.0e-52
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA
Sbjct: 448 MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 507

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 508 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 545

[Close]

BLAST of Carg02918-RA vs. NCBI nr
Match: XP_022138313.1 (aspartate--tRNA ligase 2, cytoplasmic [Momordica charantia])

HSP 1 Score: 212.6 bits (540), Expect = 5.9e-52
Identity = 97/98 (98.98%), Postives = 98/98 (100.00%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGI+VKTIETYIDSFRYGA
Sbjct: 443 MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGA 502

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 503 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540

[Close]

BLAST of Carg02918-RA vs. NCBI nr
Match: XP_022936995.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 212.6 bits (540), Expect = 5.9e-52
Identity = 97/98 (98.98%), Postives = 97/98 (98.98%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFL ERAQACGIDVKTIETYIDSFRYGA
Sbjct: 448 MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLTERAQACGIDVKTIETYIDSFRYGA 507

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 508 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 545

[Close]

BLAST of Carg02918-RA vs. NCBI nr
Match: XP_018845710.1 (PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Juglans regia])

HSP 1 Score: 204.5 bits (519), Expect = 1.6e-49
Identity = 94/98 (95.92%), Postives = 94/98 (95.92%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPC DNPTYSNSFDVFIRGEEIISGAQRVHVPEFL ERAQACGIDVKTI TYIDSFRYGA
Sbjct: 461 MPCFDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLVERAQACGIDVKTISTYIDSFRYGA 520

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP RIAP
Sbjct: 521 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPLRIAP 558

[Close]

BLAST of Carg02918-RA vs. TAIR10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 184.1 bits (466), Expect = 4.1e-47
Identity = 84/98 (85.71%), Postives = 88/98 (89.80%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           M C DNP YSNSFDVFIRGEEIISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGA
Sbjct: 461 MTCADNPLYSNSFDVFIRGEEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGA 520

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           P HGGFGVGLERVVMLFC LNNIRKTSLFPRDPQR++P
Sbjct: 521 PLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQRLSP 558

[Close]

BLAST of Carg02918-RA vs. TAIR10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 171.4 bits (433), Expect = 2.8e-43
Identity = 77/98 (78.57%), Postives = 85/98 (86.73%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDVKTI TYID+FRYGA
Sbjct: 435 MPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVKTISTYIDAFRYGA 494

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 495 PPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

[Close]

BLAST of Carg02918-RA vs. TAIR10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 68.6 bits (166), Expect = 2.5e-12
Identity = 33/94 (35.11%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYG 60
           M  +D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RYG
Sbjct: 474 MRLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYG 533

Query: 61  APPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 94
              H GFG+G ER+++   GL+NIR    FPR P
Sbjct: 534 TVKHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567

[Close]

BLAST of Carg02918-RA vs. TAIR10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 66.6 bits (161), Expect = 9.6e-12
Identity = 32/94 (34.04%), Postives = 52/94 (55.32%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYG 60
           M  +D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RYG
Sbjct: 473 MRLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYG 532

Query: 61  APPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 94
              H GFG+G ER++    G++NIR    FPR P
Sbjct: 533 TVKHCGFGLGFERMIQFATGIDNIRDVIPFPRYP 566

[Close]

BLAST of Carg02918-RA vs. TAIR10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 65.5 bits (158), Expect = 2.1e-11
Identity = 30/73 (41.10%), Postives = 42/73 (57.53%), Query Frame = 0


Query: 21  EIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 80
           E+I G+QR    E L  R     ++ ++   Y+D  RYG+ PH GFG+G ER+V    G+
Sbjct: 490 ELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGI 549

Query: 81  NNIRKTSLFPRDP 94
           +NIR    FPR P
Sbjct: 550 DNIRDVIPFPRTP 562

[Close]

BLAST of Carg02918-RA vs. Swiss-Prot
Match: sp|Q9M084|SYDC2_ARATH (Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 7.4e-46
Identity = 84/98 (85.71%), Postives = 88/98 (89.80%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           M C DNP YSNSFDVFIRGEEIISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGA
Sbjct: 461 MTCADNPLYSNSFDVFIRGEEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGA 520

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           P HGGFGVGLERVVMLFC LNNIRKTSLFPRDPQR++P
Sbjct: 521 PLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQRLSP 558

[Close]

BLAST of Carg02918-RA vs. Swiss-Prot
Match: sp|Q8H104|SYDC1_ARATH (Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g26870 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 5.0e-42
Identity = 77/98 (78.57%), Postives = 85/98 (86.73%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDVKTI TYID+FRYGA
Sbjct: 435 MPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVKTISTYIDAFRYGA 494

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 495 PPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

[Close]

BLAST of Carg02918-RA vs. Swiss-Prot
Match: sp|P14868|SYDC_HUMAN (Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS PE=1 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 3.8e-34
Identity = 63/98 (64.29%), Postives = 80/98 (81.63%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MP   NP  SNS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GA
Sbjct: 404 MPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGA 463

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPH G G+GLERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 464 PPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

[Close]

BLAST of Carg02918-RA vs. Swiss-Prot
Match: sp|Q922B2|SYDC_MOUSE (Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars PE=1 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 3.8e-34
Identity = 63/98 (64.29%), Postives = 80/98 (81.63%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MP   NP  SNS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GA
Sbjct: 404 MPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGA 463

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPH G G+GLERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 464 PPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

[Close]

BLAST of Carg02918-RA vs. Swiss-Prot
Match: sp|Q5R9I5|SYDC_PONAB (Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 3.8e-34
Identity = 63/98 (64.29%), Postives = 80/98 (81.63%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MP   NP  SNS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GA
Sbjct: 404 MPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGA 463

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPH G G+GLERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 464 PPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

[Close]

BLAST of Carg02918-RA vs. TrEMBL
Match: tr|A0A2I4GP85|A0A2I4GP85_9ROSI (aspartate--tRNA ligase 2, cytoplasmic OS=Juglans regia OX=51240 GN=LOC109009608 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-49
Identity = 94/98 (95.92%), Postives = 94/98 (95.92%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPC DNPTYSNSFDVFIRGEEIISGAQRVHVPEFL ERAQACGIDVKTI TYIDSFRYGA
Sbjct: 461 MPCFDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLVERAQACGIDVKTISTYIDSFRYGA 520

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP RIAP
Sbjct: 521 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPLRIAP 558

[Close]

BLAST of Carg02918-RA vs. TrEMBL
Match: tr|V4SEI1|V4SEI1_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10004669mg PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.3e-49
Identity = 91/98 (92.86%), Postives = 94/98 (95.92%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDN  YSNSFDVFIRGEEIISGAQR+H+PEFLAERAQACGIDVKTI TYIDSFRYGA
Sbjct: 456 MPCHDNSLYSNSFDVFIRGEEIISGAQRIHIPEFLAERAQACGIDVKTISTYIDSFRYGA 515

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSL+PRDP RIAP
Sbjct: 516 PPHGGFGVGLERVVMLFCGLNNIRKTSLYPRDPLRIAP 553

[Close]

BLAST of Carg02918-RA vs. TrEMBL
Match: tr|A0A2H5P0A9|A0A2H5P0A9_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_090130 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.3e-49
Identity = 91/98 (92.86%), Postives = 94/98 (95.92%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDN  YSNSFDVFIRGEEIISGAQR+H+PEFLAERAQACGIDVKTI TYIDSFRYGA
Sbjct: 456 MPCHDNSLYSNSFDVFIRGEEIISGAQRIHIPEFLAERAQACGIDVKTISTYIDSFRYGA 515

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSL+PRDP RIAP
Sbjct: 516 PPHGGFGVGLERVVMLFCGLNNIRKTSLYPRDPLRIAP 553

[Close]

BLAST of Carg02918-RA vs. TrEMBL
Match: tr|A0A067DVC8|A0A067DVC8_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g008808mg PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.3e-49
Identity = 91/98 (92.86%), Postives = 94/98 (95.92%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHDN  YSNSFDVFIRGEEIISGAQR+H+PEFLAERAQACGIDVKTI TYIDSFRYGA
Sbjct: 456 MPCHDNSLYSNSFDVFIRGEEIISGAQRIHIPEFLAERAQACGIDVKTISTYIDSFRYGA 515

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSL+PRDP RIAP
Sbjct: 516 PPHGGFGVGLERVVMLFCGLNNIRKTSLYPRDPLRIAP 553

[Close]

BLAST of Carg02918-RA vs. TrEMBL
Match: tr|A0A1Q3B6J0|A0A1Q3B6J0_CEPFO (tRNA-synt_2 domain-containing protein/tRNA_anti domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_07164 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.3e-49
Identity = 92/98 (93.88%), Postives = 94/98 (95.92%), Query Frame = 0


Query: 1   MPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGA 60
           MPCHD+P YSNSFDVFIRGEEIISGAQRVHVP+FLAERAQACGIDV TI TYIDSFRYGA
Sbjct: 438 MPCHDSPLYSNSFDVFIRGEEIISGAQRVHVPDFLAERAQACGIDVNTISTYIDSFRYGA 497

Query: 61  PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 99
           PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP RIAP
Sbjct: 498 PPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPLRIAP 535

The following BLAST results are available for this feature:

BLAST of Carg02918-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022976148.1	2.0e-52	100.00	aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima]	[more]
XP_023535979.1	2.0e-52	100.00	aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022138313.1	5.9e-52	98.98	aspartate--tRNA ligase 2, cytoplasmic [Momordica charantia]	[more]
XP_022936995.1	5.9e-52	98.98	aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata]	[more]
XP_018845710.1	1.6e-49	95.92	PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Juglans regia]	[more]

BLAST of Carg02918-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G31180.1	4.1e-47	85.71	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G26870.1	2.8e-43	78.57	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT5G56680.1	2.5e-12	35.11	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	9.6e-12	34.04	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	2.1e-11	41.10	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

BLAST of Carg02918-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9M084\|SYDC2_ARATH	7.4e-46	85.71	Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE...	[more]
sp\|Q8H104\|SYDC1_ARATH	5.0e-42	78.57	Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g268...	[more]
sp\|P14868\|SYDC_HUMAN	3.8e-34	64.29	Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS PE=1 SV=2	[more]
sp\|Q922B2\|SYDC_MOUSE	3.8e-34	64.29	Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars PE=1 SV=2	[more]
sp\|Q5R9I5\|SYDC_PONAB	3.8e-34	64.29	Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS PE=2 SV=1	[more]

BLAST of Carg02918-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2I4GP85\|A0A2I4GP85_9ROSI	1.1e-49	95.92	aspartate--tRNA ligase 2, cytoplasmic OS=Juglans regia OX=51240 GN=LOC109009608 ...	[more]
tr\|V4SEI1\|V4SEI1_9ROSI	5.3e-49	92.86	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10004669mg PE=3 ...	[more]
tr\|A0A2H5P0A9\|A0A2H5P0A9_CITUN	5.3e-49	92.86	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_090130 PE=3 SV=1	[more]
tr\|A0A067DVC8\|A0A067DVC8_CITSI	5.3e-49	92.86	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g008808mg PE=3 SV=1	[more]
tr\|A0A1Q3B6J0\|A0A1Q3B6J0_CEPFO	5.3e-49	93.88	tRNA-synt_2 domain-containing protein/tRNA_anti domain-containing protein OS=Cep...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006422	aspartyl-tRNA aminoacylation
GO:0006418	tRNA aminoacylation for protein translation

Vocabulary: **Molecular Function**
Term	Definition
GO:0004815	aspartate-tRNA ligase activity
GO:0005524	ATP binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0000166	nucleotide binding

Vocabulary: **INTERPRO**
Term	Definition
IPR006195	aa-tRNA-synth_II
IPR004523	Asp-tRNA_synthase_2
IPR004364	aa-tRNA-synt_II
IPR002312	Asp/Asn-tRNA-synth_IIb

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006422	aspartyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0006522	alanine metabolic process
biological_process	GO:0006531	aspartate metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0004815	aspartate-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0000166	nucleotide binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg02918	Carg02918	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg02918-RA	Carg02918-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg02918-RA.exon.1	Carg02918-RA.exon.1	exon
Carg02918-RA.exon.2	Carg02918-RA.exon.2	exon
Carg02918-RA.exon.3	Carg02918-RA.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg02918-RA.CDS.1	Carg02918-RA.CDS.1	CDS
Carg02918-RA.CDS.2	Carg02918-RA.CDS.2	CDS
Carg02918-RA.CDS.3	Carg02918-RA.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg02918-RA.three_prime_UTR.1	Carg02918-RA.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 53..67 score: 55.15 coord: 13..29 score: 40.11
None	No IPR available	GENE3D	G3DSA:3.30.930.10		coord: 1..98 e-value: 1.0E-34 score: 122.2
None	No IPR available	PANTHER	PTHR43450:SF1	ASPARTATE--TRNA LIGASE, CYTOPLASMIC	coord: 1..98
None	No IPR available	SUPERFAMILY	SSF55681	Class II aaRS and biotin synthetases	coord: 1..93
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 3..92 e-value: 4.9E-25 score: 88.2
IPR004523	Aspartate-tRNA synthetase, type 2	PANTHER	PTHR43450	FAMILY NOT NAMED	coord: 1..98
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 1..90 score: 8.733