CSPI06G30940.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G30940.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Nitric oxide synthase, inducible
Location	Chr6 : 26474026 .. 26474761 (+)
Sequence length	399

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAAACGCAATCAGATTCGGCCGTACTCCACCGCCGATTGTGCTCACATCACCTCTTCCGCCGAAGCTTCGCCGTATAAGCTCGCCTGAACTTCCATCTCGTCAAGCGATTAGTTTTGCCACTGCACCTGATCGGAATCCAAAGTGCGAAGAAATGGATGACTCTCAGAACAAAACCAAGGAGACTGGGTTAGTAGACTCCATTTTGTTGATTATTTTGGTCAATGTTTATGTTTTTTTTTAAAAAAAATTTATATAAATATTTAAACCAACTATTAAATAAAAATATGTTTTAGTTTTCAAAACAAAAGAAACAGACAACAAAATATGAAAATCTTGAAAACAAAATGATTGTCAGAATGGGACTTTATTTTTTGTTTTGTTTCTTTCATATGAAATGGGTTAATGTGTGAAAACAGAGATATAATGTGTGATTCATTCGGAGAAGGGTATGCGACCCGAAGCGAGGAAGAAGGATTTGGAGGAACTTATGGAGGGAAATATCAGTCAATTGACGATGACGAACACATGAATAAAAAACCAGTCAATCAACATGGTAATTAAACACATACACATCCTTAAAAGGAAAAAGTAAATGTGTGCATGATTTAATAGTTAAATGATTTTAATTGGTATATTGTTGTTTTGATGGTGTGATTGAAGAATATGATAAAAGCCAGGGGAGCGAAGTGAAAGAGAAGGAGAAGTCACGCCATCAAGCTCATGCAAATTGA

mRNA sequence

ATGATAAACGCAATCAGATTCGGCCGTACTCCACCGCCGATTGTGCTCACATCACCTCTTCCGCCGAAGCTTCGCCGTATAAGCTCGCCTGAACTTCCATCTCGTCAAGCGATTAGTTTTGCCACTGCACCTGATCGGAATCCAAAGTGCGAAGAAATGGATGACTCTCAGAACAAAACCAAGGAGACTGGAGATATAATGTGTGATTCATTCGGAGAAGGGTATGCGACCCGAAGCGAGGAAGAAGGATTTGGAGGAACTTATGGAGGGAAATATCAGTCAATTGACGATGACGAACACATGAATAAAAAACCAGTCAATCAACATGAATATGATAAAAGCCAGGGGAGCGAAGTGAAAGAGAAGGAGAAGTCACGCCATCAAGCTCATGCAAATTGA

Coding sequence (CDS)

ATGATAAACGCAATCAGATTCGGCCGTACTCCACCGCCGATTGTGCTCACATCACCTCTTCCGCCGAAGCTTCGCCGTATAAGCTCGCCTGAACTTCCATCTCGTCAAGCGATTAGTTTTGCCACTGCACCTGATCGGAATCCAAAGTGCGAAGAAATGGATGACTCTCAGAACAAAACCAAGGAGACTGGAGATATAATGTGTGATTCATTCGGAGAAGGGTATGCGACCCGAAGCGAGGAAGAAGGATTTGGAGGAACTTATGGAGGGAAATATCAGTCAATTGACGATGACGAACACATGAATAAAAAACCAGTCAATCAACATGAATATGATAAAAGCCAGGGGAGCGAAGTGAAAGAGAAGGAGAAGTCACGCCATCAAGCTCATGCAAATTGA

Homology

[Close]

BLAST of CSPI06G30940.1 vs. TrEMBL
Match: A0A0A0KGG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G502630 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 3.2e-67
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT 60
           MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT
Sbjct: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT 60

Query: 61  KETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVK 120
           KETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVK
Sbjct: 61  KETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVK 120

Query: 121 EKEKSRHQAHAN 133
           EKEKSRHQAHAN
Sbjct: 121 EKEKSRHQAHAN 132

[Close]

BLAST of CSPI06G30940.1 vs. TrEMBL
Match: V7CFG3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G029900g PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.6e-16
Identity = 65/134 (48.51%), Postives = 82/134 (61.19%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT 60
           M+++I+   T   + L+  L  KL R +S       A+ F T PD N    +MD S   +
Sbjct: 1   MLHSIKTVLTYQHVSLSRSLSSKLCREAS-------AVRFCTKPDSNSGAHKMDKSHKPS 60

Query: 61  KET--GDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSE 120
           +ET  GD M  SFGEGYATRS+EEGFGG YGGK QS+ + E       N   YDK+QGSE
Sbjct: 61  EETKHGDAMSHSFGEGYATRSDEEGFGGIYGGK-QSMPNTEKDKIIHENHPAYDKTQGSE 120

Query: 121 VKEKEKSRHQAHAN 133
           VKEKEK+RHQ+ AN
Sbjct: 121 VKEKEKARHQSSAN 126

[Close]

BLAST of CSPI06G30940.1 vs. TrEMBL
Match: A0A067GIH8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g046060mg PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.7e-16
Identity = 61/133 (45.86%), Postives = 77/133 (57.89%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPP-KLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNK 60
           M +AIR  R  PP VL S  P     + SS EL     +S      +N +  +  D  N 
Sbjct: 1   MNHAIRSFRANPPAVLLSSFPSCAAHKASSVELRQVTTLSKKPTNPQNMETSQKMDPNNP 60

Query: 61  TKETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEV 120
            ++TGD+M  SFGEGYATRS+EEGFGG YGG     + D+       N   +DKSQGSEV
Sbjct: 61  QEKTGDVMSHSFGEGYATRSDEEGFGGIYGGNQFFPNKDDKDKDINENHPAFDKSQGSEV 120

Query: 121 KEKEKSRHQAHAN 133
           KEKEK+RHQ +A+
Sbjct: 121 KEKEKARHQTNAS 133

[Close]

BLAST of CSPI06G30940.1 vs. TrEMBL
Match: V4SJ98_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026748mg PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.7e-16
Identity = 61/133 (45.86%), Postives = 77/133 (57.89%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPP-KLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNK 60
           M +AIR  R  PP VL S  P     + SS EL     +S      +N +  +  D  N 
Sbjct: 1   MNHAIRSFRANPPAVLLSSFPSCAAHKASSVELRQVTTLSKKPTNPQNMETSQKMDPNNP 60

Query: 61  TKETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEV 120
            ++TGD+M  SFGEGYATRS+EEGFGG YGG     + D+       N   +DKSQGSEV
Sbjct: 61  QEKTGDVMSHSFGEGYATRSDEEGFGGIYGGNQFFPNKDDKDKDINENHPAFDKSQGSEV 120

Query: 121 KEKEKSRHQAHAN 133
           KEKEK+RHQ +A+
Sbjct: 121 KEKEKARHQTNAS 133

[Close]

BLAST of CSPI06G30940.1 vs. TrEMBL
Match: A0A087GEQ9_ARAAL (Late embryogenesis abundant protein OS=Arabis alpina GN=AALP_AA8G505600 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-15
Identity = 62/134 (46.27%), Postives = 88/134 (65.67%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQA--ISFATAPDRNPKCEEMDDSQN 60
           MIN  +  R+ P   ++S        +SSP    RQ   +SFATA D++ K ++  ++QN
Sbjct: 1   MINVFKTIRSLPSTTVSS--------LSSPSRFGRQVTGVSFATASDKH-KMDKKPENQN 60

Query: 61  KTKETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSE 120
           +  +TGD+M  SFGEGYATRS+EEGFGGTYGG  Q++  D  +++   N  +YDK+QGSE
Sbjct: 61  E--KTGDVMSHSFGEGYATRSDEEGFGGTYGGN-QTLQKDNEIHE---NHPDYDKTQGSE 119

Query: 121 VKEKEKSRHQAHAN 133
            KEKEKSR+Q  ++
Sbjct: 121 AKEKEKSRNQTQSH 119

[Close]

BLAST of CSPI06G30940.1 vs. TAIR10
Match: AT5G66780.1 (AT5G66780.1 unknown protein)

HSP 1 Score: 76.3 bits (186), Expect = 1.6e-14
Identity = 51/117 (43.59%), Postives = 74/117 (63.25%), Query Frame = 1


Query: 22  PKLRRISSPELPSR-----QAISFATAPDRNPKCEEMDDS-QNKTKETGDIMCDSFGEGY 81
           P    +S    PSR       +SFAT  D+    ++MD   +N  ++TGD+M  S+GEGY
Sbjct: 12  PSNTTVSFCSTPSRLGRKVTGVSFATTSDQ----QKMDKKPENPNEKTGDVMSHSYGEGY 71

Query: 82  ATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVKEKEKSRHQAHAN 133
           ATRS+EEGFGG YGG  Q++  +  +++   N  +YDK+QGSE KEKEK+R+Q  ++
Sbjct: 72  ATRSDEEGFGGIYGGN-QTLQKENEIHE---NHPDYDKTQGSESKEKEKARNQTQSH 120

[Close]

BLAST of CSPI06G30940.1 vs. NCBI nr
Match: gi|778722467|ref|XP_011658492.1| (PREDICTED: uncharacterized protein LOC101219972 [Cucumis sativus])

HSP 1 Score: 262.3 bits (669), Expect = 4.6e-67
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT 60
           MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT
Sbjct: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT 60

Query: 61  KETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVK 120
           KETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVK
Sbjct: 61  KETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEVK 120

Query: 121 EKEKSRHQAHAN 133
           EKEKSRHQAHAN
Sbjct: 121 EKEKSRHQAHAN 132

[Close]

BLAST of CSPI06G30940.1 vs. NCBI nr
Match: gi|659081048|ref|XP_008441122.1| (PREDICTED: uncharacterized protein LOC103485350 [Cucumis melo])

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-52
Identity = 113/133 (84.96%), Postives = 119/133 (89.47%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPPKLRR-ISSPELPSRQAISFATAPDRNPKCEEMDDSQNK 60
           MINAIRFGRTPPPIVLTSPLPP+LRR I S EL SRQA  FATAPD+NPK EEMD+SQNK
Sbjct: 1   MINAIRFGRTPPPIVLTSPLPPQLRRSICSLELRSRQATGFATAPDQNPKDEEMDESQNK 60

Query: 61  TKETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEV 120
           TKETGDIMCDSFGEGYATRSEEEGFGGTYGG     DD+EH NKK VN+HEYD+SQGSEV
Sbjct: 61  TKETGDIMCDSFGEGYATRSEEEGFGGTYGGNVLDDDDEEHRNKKSVNEHEYDRSQGSEV 120

Query: 121 KEKEKSRHQAHAN 133
           KEKEKSRHQAHAN
Sbjct: 121 KEKEKSRHQAHAN 133

[Close]

BLAST of CSPI06G30940.1 vs. NCBI nr
Match: gi|593787798|ref|XP_007156938.1| (hypothetical protein PHAVU_002G029900g [Phaseolus vulgaris])

HSP 1 Score: 93.2 bits (230), Expect = 3.7e-16
Identity = 65/134 (48.51%), Postives = 82/134 (61.19%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNKT 60
           M+++I+   T   + L+  L  KL R +S       A+ F T PD N    +MD S   +
Sbjct: 1   MLHSIKTVLTYQHVSLSRSLSSKLCREAS-------AVRFCTKPDSNSGAHKMDKSHKPS 60

Query: 61  KET--GDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSE 120
           +ET  GD M  SFGEGYATRS+EEGFGG YGGK QS+ + E       N   YDK+QGSE
Sbjct: 61  EETKHGDAMSHSFGEGYATRSDEEGFGGIYGGK-QSMPNTEKDKIIHENHPAYDKTQGSE 120

Query: 121 VKEKEKSRHQAHAN 133
           VKEKEK+RHQ+ AN
Sbjct: 121 VKEKEKARHQSSAN 126

[Close]

BLAST of CSPI06G30940.1 vs. NCBI nr
Match: gi|658033897|ref|XP_008352469.1| (PREDICTED: uncharacterized protein LOC103415969 [Malus domestica])

HSP 1 Score: 91.3 bits (225), Expect = 1.4e-15
Identity = 64/134 (47.76%), Postives = 82/134 (61.19%), Query Frame = 1


Query: 1   MINAIRFGRTPPPI-VLTSPLPPKLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNK 60
           M  AIR  R  PP  + T PLP  L R +S  +  +QA S   + +   +   MD  Q+K
Sbjct: 4   MTTAIRLIRPQPPAWIFTPPLPSNLGRKASG-VRFKQAASVDVSSEPESENGNMDKQQDK 63

Query: 61  -TKETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSE 120
            T++TGD++  SFGEGYA+R ++EGFGG YGG  QS+   EH      N   YDKSQGSE
Sbjct: 64  PTEKTGDLLSHSFGEGYASRCDDEGFGGIYGGN-QSLPKTEHGQLVHENHPVYDKSQGSE 123

Query: 121 VKEKEKSRHQAHAN 133
           VKEKEK+RHQ  A+
Sbjct: 124 VKEKEKARHQTDAS 135

[Close]

BLAST of CSPI06G30940.1 vs. NCBI nr
Match: gi|567866125|ref|XP_006425685.1| (hypothetical protein CICLE_v10026748mg [Citrus clementina])

HSP 1 Score: 91.3 bits (225), Expect = 1.4e-15
Identity = 61/133 (45.86%), Postives = 77/133 (57.89%), Query Frame = 1


Query: 1   MINAIRFGRTPPPIVLTSPLPP-KLRRISSPELPSRQAISFATAPDRNPKCEEMDDSQNK 60
           M +AIR  R  PP VL S  P     + SS EL     +S      +N +  +  D  N 
Sbjct: 1   MNHAIRSFRANPPAVLLSSFPSCAAHKASSVELRQVTTLSKKPTNPQNMETSQKMDPNNP 60

Query: 61  TKETGDIMCDSFGEGYATRSEEEGFGGTYGGKYQSIDDDEHMNKKPVNQHEYDKSQGSEV 120
            ++TGD+M  SFGEGYATRS+EEGFGG YGG     + D+       N   +DKSQGSEV
Sbjct: 61  QEKTGDVMSHSFGEGYATRSDEEGFGGIYGGNQFFPNKDDKDKDINENHPAFDKSQGSEV 120

Query: 121 KEKEKSRHQAHAN 133
           KEKEK+RHQ +A+
Sbjct: 121 KEKEKARHQTNAS 133

The following BLAST results are available for this feature:

BLAST of CSPI06G30940.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI06G30940.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KGG3_CUCSA	3.2e-67	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G502630 PE=4 SV=1	[more]
V7CFG3_PHAVU	2.6e-16	48.51	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G029900g PE=4 SV=1	[more]
A0A067GIH8_CITSI	9.7e-16	45.86	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g046060mg PE=4 SV=1	[more]
V4SJ98_9ROSI	9.7e-16	45.86	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026748mg PE=4 SV=1	[more]
A0A087GEQ9_ARAAL	1.7e-15	46.27	Late embryogenesis abundant protein OS=Arabis alpina GN=AALP_AA8G505600 PE=4 SV=...	[more]

BLAST of CSPI06G30940.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G66780.1	1.6e-14	43.59	unknown protein	[more]

BLAST of CSPI06G30940.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778722467\|ref\|XP_011658492.1\|	4.6e-67	100.00	PREDICTED: uncharacterized protein LOC101219972 [Cucumis sativus]	[more]
gi\|659081048\|ref\|XP_008441122.1\|	1.1e-52	84.96	PREDICTED: uncharacterized protein LOC103485350 [Cucumis melo]	[more]
gi\|593787798\|ref\|XP_007156938.1\|	3.7e-16	48.51	hypothetical protein PHAVU_002G029900g [Phaseolus vulgaris]	[more]
gi\|658033897\|ref\|XP_008352469.1\|	1.4e-15	47.76	PREDICTED: uncharacterized protein LOC103415969 [Malus domestica]	[more]
gi\|567866125\|ref\|XP_006425685.1\|	1.4e-15	45.86	hypothetical protein CICLE_v10026748mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G30940	CSPI06G30940	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G30940.1	CSPI06G30940.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G30940.1.cds1	CSPI06G30940.1.cds1	CDS
CSPI06G30940.1.cds2	CSPI06G30940.1.cds2	CDS
CSPI06G30940.1.cds3	CSPI06G30940.1.cds3	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR36410	FAMILY NOT NAMED	coord: 1..128 score: 1.9