CSPI05G18560.2 (mRNA) Wild cucumber (PI 183967)

NameCSPI05G18560.2
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionATP-dependent clp protease ATP-binding subunit clpx, putative
LocationChr5 : 19608263 .. 19611354 (-)
Sequence length603
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAAGGAAATTCAACACCATAGTGAAAAATCTTGTATTCTTTTCAGAAAAATAAAAAGAAAAAAAGAAAAAAATACATGCATATATAACTTCCTAAATGTTGGGATTAATAAATATTTTATTTTATTTTCGTGAGGCAGGGATTAGTGTATTAATGGAGTGACACTGAAATTAAATCGGACAAAACTTCCTGTTTTAAAATTTGTTAATGCATAATTTTAATTAATGTGAATGATTTTTCATCTTCGGCAGATGGATACTAAAAATATTCTCTTCATATGTGGTGGTGCATTTGTTGGCTTGGAGAAGTGCATCTCTGACAGGTACAAAATTGCTTCGTTATAACCAATGAATTTTTGTTTTTCCCCCTTTTATAGTCCATGCATGGAAATTGAATATCAACTCTCTTTCTGCAGGCAGCATGATTCCTCAATTGGATTCGGGGCTCCAGTTCGTGCTAGCATGAGAACTGGTAAATTGACTGAAGATTCAGTGACATCTTCGATGCTTGAAAATGTGAGACCTTAAACCAAAAGTTAAATTGTTGCACTATGAATTACTTTTGTTATCTTACCTTTGGCATTTTCTGAACTGAGGTTTCATTTACTTTCATTTGTTCCCCTCCATTCAACTACTGAAATGAAGGTCTTTTAACTTTTAGGTAGAAAGTGGCGATCTTATTACTTATGGTCTCATACCTGAATTTGTTGGACGATGTCCAATTCTAGTGAGTTTGTCAGCTTTGGATGAGGATCAACTTGTCCAGGTTTGGCTAGTCTTTTGGGTCATCTTTACTTATTTCTTTGCTCTGTTTTGTTACTTAGCTCTGGAAGATATAACAAATTTCTTCATCCTTATCTCTTTAATAGGTTCTTACAAAACCAAAAAATGCGCTTGGGAAACAGTACAAGAAGATGCTTAGAATGAATGATGTAAGTATTTTAGAATGAATGATGTTGGTACAAAATATTATTAATGAACCAAGCATTTATTACATATATGAAATGTTAAGCCAAGTTACTTCTAAGAAAATAAAAGGTAAAATTACAAAGATGGTTCTTATGGTGGGTAGAAAGTTAGAATTTTAGTCCTTATGGTATGATAAAACCTCGTAAATAGTCCCTAACTTTCTCCAAACCATAGGGACCAAATTATAACTTTCTTTAACCATAGGGACCAAATTTACAATTTCCAAAATAAAAACAAAGAATAGGTTTCTAAGAACTTGTTGTAAACCCATTAGTGTTCTTGTGTGTTTACTCCAAGGCTTTTGCAGGTTGAATTACATTTCACCGAGAATGCTTTAAGAATGATTGCGAGGAAAGCAATGAAAAAGAACACAGGCGCTAGGGGATTGAGATCCATTTTAGAAAATATTCTGACAGAAGCTATGTTTGAGGTATCACATTTACGAGTGCAATGTGTTTGTTTATTCCCCATTGAATTTCTATCTTTCTAATTTCAAAATGATGAGTTCCTGAACATGTGCTTTTTCTTTCTTCAAATATTTATAAAATGTCATTACGCTCAGGTTCCAGAAAGTAACAGCATAAAGGCTGTTCTGGTTGATGAAGAAGCTGTTGGATCAGTGGACGCTTCAGGCTGCGGAGCGAAAATTCTTTGTGATGTTGATGAATTAACGCAGTGTTCAAAATCTGAAATAATGAGAAATCTGAAGGTATTTTCTAATGATCTTCACTTGAACTCTTTGTCGTGTTGATCCTTTGCTTTGGTTATGTAACTGATAAGAAACCCTTGGACATTGGATCTAAGAACCATGTTAAACAACGAGACTAAAAGGAATACAACCCCAACAGTCCCAAAACCAATTTGATTTCAACCCCTAAGATAGGCTAGATACAAATTGCAAGAACAATTTTTTTCTCAACTTTTCTTTTTGTGTTGTCTTCATAATTAACTTGTCTTGCTATGTTAATAGCTTGGATCTTTGGATTGAGGATTAGAATTGCCTTATCATTTGGCATCGAGCATAGGTTGCATCTTTGTGGCCTACTCTAGGATTGATTCTCTCTTTCTTGCAAACTATTAATTCTAGGTTTTAGTTTCATCTTATCATTTCTTGTTTTTTGATCGATCGTTCTTCATATCTTTATTCTTCATATCTTTATCTTACTGTTATTACGTTTTACATATCGAAAAAAAAACAAAGTTGTGAAGAAAAGTAAAAAAGAAAAAGAAAAACTTTAGATGAATACAATTTGTAATCTCATTTTGAGAGTTTTTCCTTAATTTGTAATTCTTGTAATAGAATCATGCGAGTCATTCAATCTAACCTTGATCAAGTTTGATTGTGGAGTGATTCAATTAGGACAAGATTTCCAAATATTGCTTCTAGATCTTTTTTATCTAAGAGGTAATCTAATCTTAACTTTCTCTTAGTTGATCTAAATTGGTATAAAGAGGTGTTAATTTCTTATTTCAAATGTTCTTGCTAGTTGATAGCTTAATAGCTTAGATCTTTAGATTGAAGATTAAGATTGCCTTGTCAAATGAATGAGCTTACCATTTCTAATGTCCTAATCTCATGCAACTCATTTCCTTACCTAGTAATAAAATAGAAATAAAAATAAATAAAAATAAAAAATCCAATGTTTTTTCTTTTATTTAAATGTGTGGCATGACAGATTTTCACTGGTGTGCGGTGGAACTAGGCAGCATCTAAGCTATTTTCACTTATTTTCTTATTTGTCCAGCATGTTCCTAAGCATGTCCATGCTAGAAAGTAATGTTTTGTTAGAAAACATTGTTCTCGTGGTGGTACTGAATGAATGATATACGTTTTGTGAGACTGAGGTGAATGATGCCAGCTTAGATTGTACGAAGGTTTTTTCTTCGTTGAATTCTCCTCATCAAATTCAAATTGTTTTAGTGGGTATGAACATACTATCTTTGCCTTGATATACAGGGAAATGATATGGTTGCCGGCAACGAGGGTCGGTTCTCAAATGGAGTCGAGTTTCCATCTGTAGCTATTAGATTGTAAATGGCTTCTGCAGTTGATGATGTAAATTTATTGAACAAATTAGTATCAACAACAACAATCAATAATAAAATGTAAGCCTTCACTTTT

mRNA sequence

ATGATTTTTCATCTTCGGCAGATGGATACTAAAAATATTCTCTTCATATGTGGTGGTGCATTTGTTGGCTTGGAGAAGTGCATCTCTGACAGGCAGCATGATTCCTCAATTGGATTCGGGGCTCCAGTTCGTGCTAGCATGAGAACTGGTAAATTGACTGAAGATTCAGTGACATCTTCGATGCTTGAAAATGTTCTTACAAAACCAAAAAATGCGCTTGGGAAACAGTACAAGAAGATGCTTAGAATGAATGATGTTGAATTACATTTCACCGAGAATGCTTTAAGAATGATTGCGAGGAAAGCAATGAAAAAGAACACAGGCGCTAGGGGATTGAGATCCATTTTAGAAAATATTCTGACAGAAGCTATGTTTGAGGTTCCAGAAAGTAACAGCATAAAGGCTGTTCTGGTTGATGAAGAAGCTGTTGGATCAGTGGACGCTTCAGGCTGCGGAGCGAAAATTCTTTGTGATGTTGATGAATTAACGCAGTGTTCAAAATCTGAAATAATGAGAAATCTGAAGGGAAATGATATGGTTGCCGGCAACGAGGGTCGGTTCTCAAATGGAGTCGAGTTTCCATCTGTAGCTATTAGATTGTAA

Coding sequence (CDS)

ATGATTTTTCATCTTCGGCAGATGGATACTAAAAATATTCTCTTCATATGTGGTGGTGCATTTGTTGGCTTGGAGAAGTGCATCTCTGACAGGCAGCATGATTCCTCAATTGGATTCGGGGCTCCAGTTCGTGCTAGCATGAGAACTGGTAAATTGACTGAAGATTCAGTGACATCTTCGATGCTTGAAAATGTTCTTACAAAACCAAAAAATGCGCTTGGGAAACAGTACAAGAAGATGCTTAGAATGAATGATGTTGAATTACATTTCACCGAGAATGCTTTAAGAATGATTGCGAGGAAAGCAATGAAAAAGAACACAGGCGCTAGGGGATTGAGATCCATTTTAGAAAATATTCTGACAGAAGCTATGTTTGAGGTTCCAGAAAGTAACAGCATAAAGGCTGTTCTGGTTGATGAAGAAGCTGTTGGATCAGTGGACGCTTCAGGCTGCGGAGCGAAAATTCTTTGTGATGTTGATGAATTAACGCAGTGTTCAAAATCTGAAATAATGAGAAATCTGAAGGGAAATGATATGGTTGCCGGCAACGAGGGTCGGTTCTCAAATGGAGTCGAGTTTCCATCTGTAGCTATTAGATTGTAA
BLAST of CSPI05G18560.2 vs. Swiss-Prot
Match: CLPX3_ARATH (CLP protease regulatory subunit CLPX3, mitochondrial OS=Arabidopsis thaliana GN=CLPX3 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 9.1e-43
Identity = 99/189 (52.38%), Postives = 123/189 (65.08%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDSVTSSML---- 66
           Q+DTK+ILFICGGAFV LEK I DR+ DSSIGFGAPVRA+M T  +T  ++TSS+L    
Sbjct: 433 QIDTKDILFICGGAFVDLEKTIVDRRQDSSIGFGAPVRANMATSGVTSGAITSSLLESVE 492

Query: 67  -------------------------------ENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                                            VL +PKNALGKQYKK+  MN+V+LHFT
Sbjct: 493 SADLTAYGLIPEFVGRFPILVSLSALTEDQLIRVLVEPKNALGKQYKKLFSMNNVKLHFT 552

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNS----IKAVLVDEEAVGSVD 157
           E AL +I+++AM KNTGARGLR++LE+ILTEAMFE+P+       I AV+VDEE+  S  
Sbjct: 553 EKALEIISKQAMVKNTGARGLRALLESILTEAMFEIPDDKKGDERIDAVIVDEESTSSEA 612

BLAST of CSPI05G18560.2 vs. Swiss-Prot
Match: CLPX2_ARATH (CLP protease regulatory subunit CLPX2, mitochondrial OS=Arabidopsis thaliana GN=CLPX2 PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.2e-42
Identity = 106/190 (55.79%), Postives = 124/190 (65.26%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSS-------------IGFG-APVRASMRTGKL 66
           QMDTK+ILFICGGAF+ LEK +S+RQHD+S              G   A V +S+     
Sbjct: 389 QMDTKDILFICGGAFIDLEKTVSERQHDASIGFGASVRTNMSTSGLSSAAVTSSLLESLQ 448

Query: 67  TEDSVTSSM---------------------LENVLTKPKNALGKQYKKMLRMNDVELHFT 126
           +ED V   +                     L  VLT+PK+ALGKQYKK+ RMN+V+L FT
Sbjct: 449 SEDLVAYGLIPEFVGRLPILVSLSALNEDQLVQVLTEPKSALGKQYKKLFRMNNVQLQFT 508

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPE-----SNSIKAVLVDEEAVGSV 157
           E A R+IARKAM KNTGARGLRSILE+ILTEAMFEVP+     S SIKAVLVDEEAVGSV
Sbjct: 509 EGATRLIARKAMSKNTGARGLRSILESILTEAMFEVPDSITEGSQSIKAVLVDEEAVGSV 568

BLAST of CSPI05G18560.2 vs. Swiss-Prot
Match: CLPX1_ARATH (CLP protease regulatory subunit CLPX1, mitochondrial OS=Arabidopsis thaliana GN=CLPX1 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.9e-38
Identity = 105/229 (45.85%), Postives = 135/229 (58.95%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIG----FGAPVRASMRT------------- 66
           Q+DTK+ILFICGGAFV +EK IS+R+HDSSIG      A +RA   T             
Sbjct: 348 QIDTKDILFICGGAFVDIEKTISERRHDSSIGFGAPVRANMRAGGVTNAAVASNLMETVE 407

Query: 67  -------GKLTE-----------DSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                  G + E            ++T + L  VLT+PKNALGKQYKKM +MN V+LHFT
Sbjct: 408 SSDLIAYGLIPEFVGRFPVLVSLSALTENQLMQVLTEPKNALGKQYKKMYQMNSVKLHFT 467

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPE----SNSIKAVLVDEEAVGSVD 186
           E+ALR+IARKA+ KNTGARGLR++LE+IL ++M+E+P+    S+ I+AV+VDEEAV    
Sbjct: 468 ESALRLIARKAITKNTGARGLRALLESILMDSMYEIPDEGTGSDMIEAVVVDEEAVEGEG 527

Query: 187 ASGCGAKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSV 197
             G GAKIL     L +   SE            G++G      E PSV
Sbjct: 528 RRGSGAKILRGKGALAR-YLSETNSKDSPQTTKEGSDGETEVEAEIPSV 575

BLAST of CSPI05G18560.2 vs. Swiss-Prot
Match: CLPX_RUEPO (ATP-dependent Clp protease ATP-binding subunit ClpX OS=Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) GN=clpX PE=3 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 3.0e-30
Identity = 73/167 (43.71%), Postives = 103/167 (61.68%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGK--------------- 66
           Q+DT NILFICGGAF GL+K I+ R   S++GFGA VRAS   G                
Sbjct: 237 QVDTTNILFICGGAFAGLDKIIAQRGKGSAMGFGADVRASDERGVGELFTELEPEDLLKF 296

Query: 67  -------------LTEDSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFTENALRMI 126
                         T + +    L  +LTKPKNAL KQY+++  + D EL FT++AL+ I
Sbjct: 297 GLIPEFVGRLPVLATLEDLDEDALVTILTKPKNALVKQYQRLFELEDTELDFTDDALKAI 356

Query: 127 ARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGS 146
           A++A+++ TGARGLRSI+E+IL + MF++P  +++  V+V+EEAV S
Sbjct: 357 AKRAIERKTGARGLRSIMEDILLDTMFDLPSMDNVTKVVVNEEAVTS 403

BLAST of CSPI05G18560.2 vs. Swiss-Prot
Match: CLPX_CHESB (ATP-dependent Clp protease ATP-binding subunit ClpX OS=Chelativorans sp. (strain BNC1) GN=clpX PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 6.7e-30
Identity = 76/165 (46.06%), Postives = 100/165 (60.61%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRA--SMRTGKL------------ 66
           Q+DT +ILFICGGAF GLEK ISDR   +SIGFGA V +    R G+L            
Sbjct: 238 QVDTSSILFICGGAFAGLEKIISDRGKKTSIGFGASVSSPEDRRAGELLRQVEPEDLLKF 297

Query: 67  --------------TEDSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFTENALRMI 126
                         T + +    L  +LT+PKNAL KQY+++  M +VEL F ENALR I
Sbjct: 298 GLIPEFVGRLPILATLEDLDEEALVQILTEPKNALVKQYQRLFEMENVELTFHENALRAI 357

Query: 127 ARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAV 144
           ARKA+++ TGARGLRSI+E IL + MFE+P    ++ V++ E+ V
Sbjct: 358 ARKAIERKTGARGLRSIMEAILLDTMFELPALEGVQEVVISEDVV 402

BLAST of CSPI05G18560.2 vs. TrEMBL
Match: A0A0A0KR77_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G527380 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 4.2e-87
Identity = 179/228 (78.51%), Postives = 182/228 (79.82%), Query Frame = 1

Query: 8   MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT------------------ 67
           MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT                  
Sbjct: 1   MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDLVTSSMLENVES 60

Query: 68  ------GKLTE-----------DSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFTE 127
                 G + E            ++    L  VLTKPKNALGKQYKKMLRMNDVELHFTE
Sbjct: 61  GDLITYGLIPEFVGRCPILVSLSALDEDQLVQVLTKPKNALGKQYKKMLRMNDVELHFTE 120

Query: 128 NALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGCG 187
           NALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGCG
Sbjct: 121 NALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGCG 180

Query: 188 AKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSVAIRL 201
           AKILCDVDELTQCSKSEIMRNLK NDMVAGNEGR SNGVEFPSVAIRL
Sbjct: 181 AKILCDVDELTQCSKSEIMRNLKVNDMVAGNEGRLSNGVEFPSVAIRL 228

BLAST of CSPI05G18560.2 vs. TrEMBL
Match: K7N4V3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_20G213400 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-53
Identity = 109/155 (70.32%), Postives = 135/155 (87.10%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDSVTSSMLENVL 66
           Q+DTKNILFICGGAF+ LEK IS+R+ DSSIGFG+PVR++MRTG +T  +VTSS+LE+VL
Sbjct: 284 QIDTKNILFICGGAFIDLEKTISERRQDSSIGFGSPVRSNMRTGGVTNAAVTSSLLESVL 343

Query: 67  TKPKNALGKQYKKMLRMNDVELHFTENALRMIARKAMKKNTGARGLRSILENILTEAMFE 126
           T+PKNAL KQYKK+  MN+V+LHFTE ALR+IA+KAM KNTGARGLR++LE+ILTEAMFE
Sbjct: 344 TEPKNALAKQYKKLFNMNNVKLHFTEKALRLIAKKAMAKNTGARGLRALLESILTEAMFE 403

Query: 127 VPE----SNSIKAVLVDEEAVGSVDASGCGAKILC 158
           +P+    +  I AV+VDEE+VGSV+A GCG KILC
Sbjct: 404 IPDVKAGNELIGAVVVDEESVGSVNAPGCGGKILC 438

BLAST of CSPI05G18560.2 vs. TrEMBL
Match: A0A0R0HUU5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G176800 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 6.3e-51
Identity = 105/159 (66.04%), Postives = 132/159 (83.02%), Query Frame = 1

Query: 3   FHLRQMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDSVTSSML 62
           F+  Q+DTKNILFICGGAF+ LEK IS+R+ DSSIGFGAPVR++MR G +T  +VTSS+L
Sbjct: 136 FYDMQIDTKNILFICGGAFINLEKTISERRQDSSIGFGAPVRSNMRAGGITNAAVTSSLL 195

Query: 63  ENVLTKPKNALGKQYKKMLRMNDVELHFTENALRMIARKAMKKNTGARGLRSILENILTE 122
           E+VL +PKNAL KQYKK+   N+V+LHFTE A R+IA+KAM K TGARGLR++LE+ILTE
Sbjct: 196 ESVLIEPKNALAKQYKKLFNTNNVKLHFTEKAHRLIAKKAMAKKTGARGLRALLESILTE 255

Query: 123 AMFEVPESNS----IKAVLVDEEAVGSVDASGCGAKILC 158
           AM+E+P+  +    I AV+VDEE+VGSV+A GCG KILC
Sbjct: 256 AMYEIPDVKAGDELIDAVVVDEESVGSVNAPGCGGKILC 294

BLAST of CSPI05G18560.2 vs. TrEMBL
Match: A0A0B2RFB0_GLYSO (ATP-dependent Clp protease ATP-binding subunit ClpX (Fragment) OS=Glycine soja GN=glysoja_029969 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 7.0e-50
Identity = 103/154 (66.88%), Postives = 129/154 (83.77%), Query Frame = 1

Query: 8   MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDSVTSSMLENVLT 67
           +DTKNILFICGGAF+ LEK IS+R+ DSSIGFGAPVR++MR G +T  +VTSS+LE+VL 
Sbjct: 52  IDTKNILFICGGAFINLEKTISERRQDSSIGFGAPVRSNMRAGGITNAAVTSSLLESVLI 111

Query: 68  KPKNALGKQYKKMLRMNDVELHFTENALRMIARKAMKKNTGARGLRSILENILTEAMFEV 127
           +PKNAL KQYKK+   N+V+LHFTE A R+IA+KAM K TGARGLR++LE+ILTEAM+E+
Sbjct: 112 EPKNALAKQYKKLFNTNNVKLHFTEKAHRLIAKKAMAKKTGARGLRALLESILTEAMYEI 171

Query: 128 PESNS----IKAVLVDEEAVGSVDASGCGAKILC 158
           P+  +    I AV+VDEE+VGSV+A GCG KILC
Sbjct: 172 PDVKAGDELIDAVVVDEESVGSVNAPGCGGKILC 205

BLAST of CSPI05G18560.2 vs. TrEMBL
Match: A0A067L0Y3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24892 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 122/218 (55.96%), Postives = 143/218 (65.60%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGK--------------- 66
           QMDTK+ILFICGGAFV LEK IS+R+ D+SIGFGAPVRA+MRTG                
Sbjct: 368 QMDTKDILFICGGAFVNLEKTISERRQDASIGFGAPVRANMRTGGMTDAAVTSSLLESVE 427

Query: 67  --------------------LTEDSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                               ++  ++    L  VL +PKNALGKQYKKM  MN+V+LHFT
Sbjct: 428 SGDLIAYGLIPEFVGRFPILVSLSALNEDQLVQVLMEPKNALGKQYKKMFNMNNVKLHFT 487

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPE----SNSIKAVLVDEEAVGSVD 185
           EN+LR+IA+KAM KNTGARGLR+ILENILTEAM+EVPE    +N I AVLVDEEAVGSVD
Sbjct: 488 ENSLRLIAKKAMAKNTGARGLRAILENILTEAMYEVPENKTGTNYITAVLVDEEAVGSVD 547

BLAST of CSPI05G18560.2 vs. TAIR10
Match: AT1G33360.1 (AT1G33360.1 ATP-dependent Clp protease)

HSP 1 Score: 174.9 bits (442), Expect = 5.1e-44
Identity = 99/189 (52.38%), Postives = 123/189 (65.08%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDSVTSSML---- 66
           Q+DTK+ILFICGGAFV LEK I DR+ DSSIGFGAPVRA+M T  +T  ++TSS+L    
Sbjct: 433 QIDTKDILFICGGAFVDLEKTIVDRRQDSSIGFGAPVRANMATSGVTSGAITSSLLESVE 492

Query: 67  -------------------------------ENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                                            VL +PKNALGKQYKK+  MN+V+LHFT
Sbjct: 493 SADLTAYGLIPEFVGRFPILVSLSALTEDQLIRVLVEPKNALGKQYKKLFSMNNVKLHFT 552

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNS----IKAVLVDEEAVGSVD 157
           E AL +I+++AM KNTGARGLR++LE+ILTEAMFE+P+       I AV+VDEE+  S  
Sbjct: 553 EKALEIISKQAMVKNTGARGLRALLESILTEAMFEIPDDKKGDERIDAVIVDEESTSSEA 612

BLAST of CSPI05G18560.2 vs. TAIR10
Match: AT5G49840.1 (AT5G49840.1 ATP-dependent Clp protease)

HSP 1 Score: 174.5 bits (441), Expect = 6.7e-44
Identity = 106/190 (55.79%), Postives = 124/190 (65.26%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSS-------------IGFG-APVRASMRTGKL 66
           QMDTK+ILFICGGAF+ LEK +S+RQHD+S              G   A V +S+     
Sbjct: 389 QMDTKDILFICGGAFIDLEKTVSERQHDASIGFGASVRTNMSTSGLSSAAVTSSLLESLQ 448

Query: 67  TEDSVTSSM---------------------LENVLTKPKNALGKQYKKMLRMNDVELHFT 126
           +ED V   +                     L  VLT+PK+ALGKQYKK+ RMN+V+L FT
Sbjct: 449 SEDLVAYGLIPEFVGRLPILVSLSALNEDQLVQVLTEPKSALGKQYKKLFRMNNVQLQFT 508

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPE-----SNSIKAVLVDEEAVGSV 157
           E A R+IARKAM KNTGARGLRSILE+ILTEAMFEVP+     S SIKAVLVDEEAVGSV
Sbjct: 509 EGATRLIARKAMSKNTGARGLRSILESILTEAMFEVPDSITEGSQSIKAVLVDEEAVGSV 568

BLAST of CSPI05G18560.2 vs. TAIR10
Match: AT5G53350.1 (AT5G53350.1 CLP protease regulatory subunit X)

HSP 1 Score: 159.5 bits (402), Expect = 2.2e-39
Identity = 105/229 (45.85%), Postives = 135/229 (58.95%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIG----FGAPVRASMRT------------- 66
           Q+DTK+ILFICGGAFV +EK IS+R+HDSSIG      A +RA   T             
Sbjct: 348 QIDTKDILFICGGAFVDIEKTISERRHDSSIGFGAPVRANMRAGGVTNAAVASNLMETVE 407

Query: 67  -------GKLTE-----------DSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                  G + E            ++T + L  VLT+PKNALGKQYKKM +MN V+LHFT
Sbjct: 408 SSDLIAYGLIPEFVGRFPVLVSLSALTENQLMQVLTEPKNALGKQYKKMYQMNSVKLHFT 467

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPE----SNSIKAVLVDEEAVGSVD 186
           E+ALR+IARKA+ KNTGARGLR++LE+IL ++M+E+P+    S+ I+AV+VDEEAV    
Sbjct: 468 ESALRLIARKAITKNTGARGLRALLESILMDSMYEIPDEGTGSDMIEAVVVDEEAVEGEG 527

Query: 187 ASGCGAKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSV 197
             G GAKIL     L +   SE            G++G      E PSV
Sbjct: 528 RRGSGAKILRGKGALAR-YLSETNSKDSPQTTKEGSDGETEVEAEIPSV 575

BLAST of CSPI05G18560.2 vs. NCBI nr
Match: gi|449450117|ref|XP_004142810.1| (PREDICTED: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 330.9 bits (847), Expect = 1.6e-87
Identity = 180/229 (78.60%), Postives = 183/229 (79.91%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT----------------- 66
           QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT                 
Sbjct: 391 QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDLVTSSMLENVE 450

Query: 67  -------GKLTE-----------DSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                  G + E            ++    L  VLTKPKNALGKQYKKMLRMNDVELHFT
Sbjct: 451 SGDLITYGLIPEFVGRCPILVSLSALDEDQLVQVLTKPKNALGKQYKKMLRMNDVELHFT 510

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC 186
           ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC
Sbjct: 511 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC 570

Query: 187 GAKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSVAIRL 201
           GAKILCDVDELTQCSKSEIMRNLK NDMVAGNEGR SNGVEFPSVAIRL
Sbjct: 571 GAKILCDVDELTQCSKSEIMRNLKVNDMVAGNEGRLSNGVEFPSVAIRL 619

BLAST of CSPI05G18560.2 vs. NCBI nr
Match: gi|778703780|ref|XP_011655426.1| (PREDICTED: mitochondrial clpX-like chaperone MCX1 isoform X3 [Cucumis sativus])

HSP 1 Score: 330.9 bits (847), Expect = 1.6e-87
Identity = 180/229 (78.60%), Postives = 183/229 (79.91%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT----------------- 66
           QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT                 
Sbjct: 310 QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDLVTSSMLENVE 369

Query: 67  -------GKLTE-----------DSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
                  G + E            ++    L  VLTKPKNALGKQYKKMLRMNDVELHFT
Sbjct: 370 SGDLITYGLIPEFVGRCPILVSLSALDEDQLVQVLTKPKNALGKQYKKMLRMNDVELHFT 429

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC 186
           ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC
Sbjct: 430 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC 489

Query: 187 GAKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSVAIRL 201
           GAKILCDVDELTQCSKSEIMRNLK NDMVAGNEGR SNGVEFPSVAIRL
Sbjct: 490 GAKILCDVDELTQCSKSEIMRNLKVNDMVAGNEGRLSNGVEFPSVAIRL 538

BLAST of CSPI05G18560.2 vs. NCBI nr
Match: gi|700196222|gb|KGN51399.1| (hypothetical protein Csa_5G527380 [Cucumis sativus])

HSP 1 Score: 328.9 bits (842), Expect = 6.0e-87
Identity = 179/228 (78.51%), Postives = 182/228 (79.82%), Query Frame = 1

Query: 8   MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT------------------ 67
           MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT                  
Sbjct: 1   MDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTEDLVTSSMLENVES 60

Query: 68  ------GKLTE-----------DSVTSSMLENVLTKPKNALGKQYKKMLRMNDVELHFTE 127
                 G + E            ++    L  VLTKPKNALGKQYKKMLRMNDVELHFTE
Sbjct: 61  GDLITYGLIPEFVGRCPILVSLSALDEDQLVQVLTKPKNALGKQYKKMLRMNDVELHFTE 120

Query: 128 NALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGCG 187
           NALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGCG
Sbjct: 121 NALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGCG 180

Query: 188 AKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSVAIRL 201
           AKILCDVDELTQCSKSEIMRNLK NDMVAGNEGR SNGVEFPSVAIRL
Sbjct: 181 AKILCDVDELTQCSKSEIMRNLKVNDMVAGNEGRLSNGVEFPSVAIRL 228

BLAST of CSPI05G18560.2 vs. NCBI nr
Match: gi|659121297|ref|XP_008460588.1| (PREDICTED: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial isoform X3 [Cucumis melo])

HSP 1 Score: 323.9 bits (829), Expect = 1.9e-85
Identity = 175/227 (77.09%), Postives = 183/227 (80.62%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTE--------DSVT 66
           QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT +LTE        ++V 
Sbjct: 363 QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTARLTEDLVTSSMLENVE 422

Query: 67  SS---------------------------MLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
           SS                            L  VLTKPKNALGKQYKKM RMNDVELHFT
Sbjct: 423 SSDLVTYGLIPEFVGRCPILVSLSALDEDQLVQVLTKPKNALGKQYKKMFRMNDVELHFT 482

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC 186
           ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVD E+VGSVDA GC
Sbjct: 483 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDGESVGSVDAPGC 542

Query: 187 GAKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSVAI 199
           GAKILCDVDELT+CSKSEI+RNLKGNDMVA +EGRFSNGVEFPSVA+
Sbjct: 543 GAKILCDVDELTKCSKSEIIRNLKGNDMVADDEGRFSNGVEFPSVAM 589

BLAST of CSPI05G18560.2 vs. NCBI nr
Match: gi|659121295|ref|XP_008460587.1| (PREDICTED: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 323.9 bits (829), Expect = 1.9e-85
Identity = 175/227 (77.09%), Postives = 183/227 (80.62%), Query Frame = 1

Query: 7   QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTGKLTE--------DSVT 66
           QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRT +LTE        ++V 
Sbjct: 385 QMDTKNILFICGGAFVGLEKCISDRQHDSSIGFGAPVRASMRTARLTEDLVTSSMLENVE 444

Query: 67  SS---------------------------MLENVLTKPKNALGKQYKKMLRMNDVELHFT 126
           SS                            L  VLTKPKNALGKQYKKM RMNDVELHFT
Sbjct: 445 SSDLVTYGLIPEFVGRCPILVSLSALDEDQLVQVLTKPKNALGKQYKKMFRMNDVELHFT 504

Query: 127 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDEEAVGSVDASGC 186
           ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVD E+VGSVDA GC
Sbjct: 505 ENALRMIARKAMKKNTGARGLRSILENILTEAMFEVPESNSIKAVLVDGESVGSVDAPGC 564

Query: 187 GAKILCDVDELTQCSKSEIMRNLKGNDMVAGNEGRFSNGVEFPSVAI 199
           GAKILCDVDELT+CSKSEI+RNLKGNDMVA +EGRFSNGVEFPSVA+
Sbjct: 565 GAKILCDVDELTKCSKSEIIRNLKGNDMVADDEGRFSNGVEFPSVAM 611

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CLPX3_ARATH9.1e-4352.38CLP protease regulatory subunit CLPX3, mitochondrial OS=Arabidopsis thaliana GN=... [more]
CLPX2_ARATH1.2e-4255.79CLP protease regulatory subunit CLPX2, mitochondrial OS=Arabidopsis thaliana GN=... [more]
CLPX1_ARATH3.9e-3845.85CLP protease regulatory subunit CLPX1, mitochondrial OS=Arabidopsis thaliana GN=... [more]
CLPX_RUEPO3.0e-3043.71ATP-dependent Clp protease ATP-binding subunit ClpX OS=Ruegeria pomeroyi (strain... [more]
CLPX_CHESB6.7e-3046.06ATP-dependent Clp protease ATP-binding subunit ClpX OS=Chelativorans sp. (strain... [more]
Match NameE-valueIdentityDescription
A0A0A0KR77_CUCSA4.2e-8778.51Uncharacterized protein OS=Cucumis sativus GN=Csa_5G527380 PE=4 SV=1[more]
K7N4V3_SOYBN1.0e-5370.32Uncharacterized protein OS=Glycine max GN=GLYMA_20G213400 PE=4 SV=1[more]
A0A0R0HUU5_SOYBN6.3e-5166.04Uncharacterized protein OS=Glycine max GN=GLYMA_10G176800 PE=4 SV=1[more]
A0A0B2RFB0_GLYSO7.0e-5066.88ATP-dependent Clp protease ATP-binding subunit ClpX (Fragment) OS=Glycine soja G... [more]
A0A067L0Y3_JATCU2.0e-4955.96Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24892 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G33360.15.1e-4452.38 ATP-dependent Clp protease[more]
AT5G49840.16.7e-4455.79 ATP-dependent Clp protease[more]
AT5G53350.12.2e-3945.85 CLP protease regulatory subunit X[more]
Match NameE-valueIdentityDescription
gi|449450117|ref|XP_004142810.1|1.6e-8778.60PREDICTED: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondri... [more]
gi|778703780|ref|XP_011655426.1|1.6e-8778.60PREDICTED: mitochondrial clpX-like chaperone MCX1 isoform X3 [Cucumis sativus][more]
gi|700196222|gb|KGN51399.1|6.0e-8778.51hypothetical protein Csa_5G527380 [Cucumis sativus][more]
gi|659121297|ref|XP_008460588.1|1.9e-8577.09PREDICTED: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondri... [more]
gi|659121295|ref|XP_008460587.1|1.9e-8577.09PREDICTED: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondri... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR019489Clp_ATPase_C
IPR027417P-loop_NTPase
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0008233 peptidase activity
molecular_function GO:0051082 unfolded protein binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI05G18560CSPI05G18560gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI05G18560.2CSPI05G18560.2-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G18560.2.utr3p1CSPI05G18560.2.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G18560.2.cds6CSPI05G18560.2.cds6CDS
CSPI05G18560.2.cds5CSPI05G18560.2.cds5CDS
CSPI05G18560.2.cds4CSPI05G18560.2.cds4CDS
CSPI05G18560.2.cds3CSPI05G18560.2.cds3CDS
CSPI05G18560.2.cds2CSPI05G18560.2.cds2CDS
CSPI05G18560.2.cds1CSPI05G18560.2.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G18560.2.utr5p1CSPI05G18560.2.utr5p1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019489Clp ATPase, C-terminalPFAMPF10431ClpB_D2-smallcoord: 59..134
score: 1.7
IPR019489Clp ATPase, C-terminalSMARTSM01086ClpB_D2_small_2coord: 57..147
score: 8.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 8..128
score: 3.4
NoneNo IPR availableGENE3DG3DSA:1.10.8.60coord: 58..144
score: 4.5
NoneNo IPR availablePANTHERPTHR11262HSL AND CLP PROTEASEcoord: 7..156
score: 2.8
NoneNo IPR availablePANTHERPTHR11262:SF9ATP-DEPENDENT CLP PROTEASEcoord: 7..156
score: 2.8