CSPI04G20130.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI04G20130.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionE3 ubiquitin-protein ligase CHFR
LocationChr4 : 18188790 .. 18192468 (-)
Sequence length258
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTGTTTCATTTCAAAATTCTCAATCTTCTCCTTCTCCTACTGGCGGTATGGCGGAAATTGGAGAGTGCTCTGCTTCAAATTCATCCATGGAAAACTGGGCGAAGCTAAGTATGTTTCATCTTCCCATGTCTCTCCTCCCTGAGTTCTTCTCAGTCTGTCTCTTGTTTGACTGATCCGATGGCTAATCCGTTTTGCATTAGTGGATTCTCTGTTTCATTTGCTTTTGCTCAGCATTTGAGAATTGGGCATTTTTTTTATAATTTTTTTTGCATTCTTTGCAAACAAAAATTTGTTACTATCCTGAAAAAGTTTTCTTCAGTTTGAAGTCTGGGTTGAAATTACTGGGGAAGATAATGTCATCGAGGAAATAATTTTGGTGGTACGGGAGTTGAACTAGGTTTGACAATGATGGAAGTTCAATGTTTCATTTGTATTTGATTTCATGAACTGTTAGCAGTATGATTTACATAGTAGAAGGTAATTGTCTCACTAATGTTTCTTTCGGTGTTTTAAATTCATTTTAGGGAAATAGGCAGACAGTATAGAAATTAAAGAATTTCTATTTTGATGAAAGATATACAAGAGGAAGAAGAACGATTAGGAATCGTACTCTCCCACTTAACTTAAATGATCACAGAAAGCATGGGAAAATTTTACAAGAACATCCATGCAAAGAACACATCTAGTCATCGAAAACGATCAAATGAATCAAAGTTTATTTAACCTCTCTGAATTTATAGCTCAAAATCTATATATCTTCCATTCCAATTTTCTCAAAATTTTTTTACTTTAACATGAAAAAGTGACGGGATAAAGCTTTCACGTTCTTAGAAGGATTCCCATACCCTTGAGGAAATGATTACATTAAAGTTTGTCTCAATTTCCATGAACAAGGTAGTTGTAATACGATCATGGATTTGCATGAATTAAAATTGATGGTGGATTGAAAATTATTCTTGAACATATTTGTCATTAAAATATATGTATTTATATTAGAAAAAAAAACAAGAAACACAAAATTTCACAAATGATTGAAAATTTATAAAAGAGTACAAGGATTCAAAGACTACAATGTGTAGACGTGGGAAAACAAAAGTTGCAAGTGGAAGAAAATTGCAAAATAGAAAGAGGGAAAGCTATAACTTGACCATAAAAAAAGCCTCTCAATCGTTTATTGTCATTTACAGATCTTAATATCAAGATTACACCTTTGGGATGATTTCCTTTCAAGAAGCCTTTCTTTCAATTTCCTATGTTGGAATTTTGGTTGCTACCACTTATCTTGAATAGCTATTTTTCTTCCTCTAATACCAAAAAATGATGTAATCTAGCATCTAAGTGAATAATCTCGAAGAATACTCAACCAAGCTGTTACTGTTATTCAAACAGTATTTTAATTACAAGAGATTATCCCTTTATTTTAGTTTGAATTTGTTGGTTTTAATTTGTTATCTGTTAGTTCTCAATACTTAGTCGGTCCCCCTTCGGCTAGTTGGGGTGTTTTGGTGGGCTGGTTGTTTTTGTATGCCCATGTATCCTTTTATTTTTTCTCAATGAAAGCAGGTTGTTTTATCAAAAGAAAAAAAAAAGAAGAAAAAAACAAATTAAAACTAAATTACAAGGATACCTAGTCATGCTACATCATACTTGTCTTGTGTGTTCCTGGATTGTGCATTTTCTTACTTTATTCTTTAGGTTTTTTTAGTAGACTGCAGACATTGGTTATTAATAATTTCTATGAAAGAGTTATTTTTAAATTTATTGGAAAATTTTAAAATATCTATTACATACCACTAGGTTCGCTATCATAATTGTTTAGCAATTCCTTGAGCATAAGCTAATAACTATAAAGTTCAGAAATTTATTGTTTATGCTTTTTTAACCAAATATGAAGTTGAAAGGTTTTGTTGAGTTTAGGCTTACATGTGGGAGAGTGAACAAAATTTTAGGCTTACGTTTAGAGGCATTTACTTTCTTATAAAGGAGAAAAATGAAAAAATAGTTTAATTAAAAGTTAAGACCTTTTAATATGGAATAAACATTTATCCATTATTTATTCATTTATTATAAGAAACTGAATATCATCAAAAGGATAAGTAAATGTTTATATGAATCCACATGTTTTAATATGTACTTAGGTATGTTTCTATAATTTCTTCAATTTTTTTTCTTTTTGAAATGAGATGGAAAATTTCCGTTGACAAAATGAAAAATTACATATAAGATGTTTGCAAGCTCCAAGTGTTTCTTGGCATACAATGCAAACAAGAAGAGAAGTACTATGTGAGAGCCATTGTTTAGAGGTATATTAGCACTATTGGTATGAATATGAGCAAGGGCATATTAGTAATAGCTAGTAAGTTGGTTAGGACTTTTAGTTACAAATAGAGGGAGTGGGATGGGGAGGAAGGTGTGGAGGTTTTAGTAAAATTTCCAGTGGAGAATTTGGGAGAGAGTAGCCCTCTTGATATTGCTCTTATATTTTAGTTTCTTCATTGATATTACAATTATATTTCTATCTTCCTATTTTTTTTATATTCTTTGTTGTTCTCGAGTGTTCTTGTTAGATGGGAACCCTAAATACTCAAATGTTTATAATCTATTGCAATTTTCTAATTAAAGAAATGAAAAAAACTACTGCATTATATATTGGCATCATTTTTTGAAGTTAATTAGCCATCTTATTTTTTTCTTTTCCAGTTCCAACTGATAAAATGTATGCGGATATTGAGATAAACTCTGATGAGACGGTTATCTTCTCGGAGACAAAATCCACATCTGTTGAAAAACATGAATGGTGCAAAATCACAAGGAATTCAGATCTAAATACTGCCACTCTGCAGAATAGAAGGTAATATGCTGTACAACAAAGTAATTAGTGTTTGAATCAAATGATATCCTTATCCCCATAAATCAAAGCTCCAATACGTTTTTGGTCCAGGCTTTTAAGATCTCATATAAAATTGTAAACCTTAGAAAAATTTTCCACTTTTACCCTTAAGGACTCTATTGGAGCTTTGGTATCAAAGTTCAGTAAACAAATACGATGAAGTTTCATGATTTTACACTGATAGCAGATTAGCTAGCTGATAGGAAGACCATGGGAGTTAGGTGTAAATTTCACCCAGGATCTTGACTTTTAGTGTTTAGGTAGAATGTTGATGAGAAAATTTTGTTTTCTCAATAATCTTGCTTTTATTTTTCAATGTTGTTGTAACCCTAAACCCTAATTTTTTTTGAAAAGGAAACCAGCCTCTTTATTGATAAGAATAACGTATTTCAAGGTACAAAAGGTTTATATTTTTTTTGAAATGGAGACAAGAATTTCTTTATTAAAAATAACTCAAAGTACAAGAGAGTTAGAGTCAGACAAAGAGAGCCAAAAAGAAATAGTAATCAAAGGAGACCAAGAGGGATTAGGAGGTGCACCCAGACATCTCAACTAGGTTGACACCGCCTTAGTGCTCTCACATTATATCCAACAAAAATAATACCCAAAGTACAAATTAAAGTCTGTCCAGAGTACAAACAACATGTGCTGTGGAGCCAAACAAAAACTACAAACAACATCGAAAAAACAGCAGCAGTACAAATCAGTAAAACAATTAATCTTTCAGTGGTGGCTGCAGACCACCATTCATGAAGAAAGTGACTACTAATGATTTGGTAAAATGA

mRNA sequence

ATGGCGGAAATTGGAGAGTGCTCTGCTTCAAATTCATCCATGGAAAACTGGGCGAAGCTAATTCCAACTGATAAAATGTATGCGGATATTGAGATAAACTCTGATGAGACGGTTATCTTCTCGGAGACAAAATCCACATCTGTTGAAAAACATGAATGGTGCAAAATCACAAGGAATTCAGATCTAAATACTGCCACTCTGCAGAATAGAAGACCACCATTCATGAAGAAAGTGACTACTAATGATTTGGTAAAATGA

Coding sequence (CDS)

ATGGCGGAAATTGGAGAGTGCTCTGCTTCAAATTCATCCATGGAAAACTGGGCGAAGCTAATTCCAACTGATAAAATGTATGCGGATATTGAGATAAACTCTGATGAGACGGTTATCTTCTCGGAGACAAAATCCACATCTGTTGAAAAACATGAATGGTGCAAAATCACAAGGAATTCAGATCTAAATACTGCCACTCTGCAGAATAGAAGACCACCATTCATGAAGAAAGTGACTACTAATGATTTGGTAAAATGA
BLAST of CSPI04G20130.1 vs. TrEMBL
Match: A0A0A0KYR0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G508530 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 2.4e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

BLAST of CSPI04G20130.1 vs. TrEMBL
Match: A0A067F5J8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g019461mg PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 7.4e-17
Identity = 44/70 (62.86%), Postives = 57/70 (81.43%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAE+GECSAS  S E WAKL P+D  +AD++I+S+E VI SE  S+S +KHEWCKITRNS
Sbjct: 1  MAEVGECSASKPSREIWAKLEPSDSRFADVDISSNEVVICSEITSSSSDKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DL++A +QN+
Sbjct: 61 DLHSAKMQNK 70

BLAST of CSPI04G20130.1 vs. TrEMBL
Match: B9N5A6_POPTR (Zinc finger family protein OS=Populus trichocarpa GN=POPTR_0004s14500g PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.4e-15
Identity = 44/71 (61.97%), Postives = 56/71 (78.87%), Query Frame = 1

Query: 3  EIGECSA---SNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRN 62
          E GECS+   S+SS E WAKL+P+D  Y+D+EI S+E VI SE  STS+EKHEWCKITRN
Sbjct: 2  EFGECSSGSNSSSSNEAWAKLVPSDSRYSDVEIRSNEMVICSEITSTSLEKHEWCKITRN 61

Query: 63 SDLNTATLQNR 71
          SD ++A +QN+
Sbjct: 62 SDQSSAMMQNK 72

BLAST of CSPI04G20130.1 vs. TrEMBL
Match: V4SGG1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024761mg PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.1e-15
Identity = 41/67 (61.19%), Postives = 54/67 (80.60%), Query Frame = 1

Query: 4   IGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNSDLN 63
           +GECSAS  S E WAKL P+D  +AD++I+S+E VI SE  S+S +KHEWCKITRNSDL+
Sbjct: 729 VGECSASKPSREIWAKLEPSDSRFADVDISSNEVVICSEITSSSSDKHEWCKITRNSDLH 788

Query: 64  TATLQNR 71
           +A +QN+
Sbjct: 789 SAKMQNK 795

BLAST of CSPI04G20130.1 vs. TrEMBL
Match: A0A0S3QY52_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G065300 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.7e-13
Identity = 40/73 (54.79%), Postives = 53/73 (72.60%), Query Frame = 1

Query: 1  MAEIGECSASNSSM---ENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKIT 60
          M E GECS+S S+    E WAKL+P+D+ Y+D+EI SDE VI SE  + S +KH WCKI 
Sbjct: 1  MEETGECSSSTSAKHLDEIWAKLVPSDERYSDVEIRSDEKVISSEISANSSDKHSWCKIV 60

Query: 61 RNSDLNTATLQNR 71
          RN DL +AT++N+
Sbjct: 61 RNPDLCSATMENK 73

BLAST of CSPI04G20130.1 vs. TAIR10
Match: AT1G47570.1 (AT1G47570.1 RING/U-box superfamily protein)

HSP 1 Score: 70.5 bits (171), Expect = 5.8e-13
Identity = 34/70 (48.57%), Postives = 50/70 (71.43%), Query Frame = 1

Query: 2  AEIGECSASNSSMEN-WAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 61
          AE G+ S S  S ++ WAKL+P D  ++DIEI  ++ VI SE K +S+EKHEWC+IT+N 
Sbjct: 5  AETGQSSGSKPSDDDAWAKLVPLDTRFSDIEIRCNDMVICSEIKPSSLEKHEWCRITKNL 64

Query: 62 DLNTATLQNR 71
            ++AT+ N+
Sbjct: 65 GQSSATIHNK 74

BLAST of CSPI04G20130.1 vs. NCBI nr
Match: gi|778695005|ref|XP_011653909.1| (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

BLAST of CSPI04G20130.1 vs. NCBI nr
Match: gi|700199667|gb|KGN54825.1| (hypothetical protein Csa_4G508530 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

BLAST of CSPI04G20130.1 vs. NCBI nr
Match: gi|778695009|ref|XP_004146401.2| (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

BLAST of CSPI04G20130.1 vs. NCBI nr
Match: gi|778695012|ref|XP_011653910.1| (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-31
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

BLAST of CSPI04G20130.1 vs. NCBI nr
Match: gi|659082892|ref|XP_008442085.1| (PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis melo])

HSP 1 Score: 139.8 bits (351), Expect = 2.2e-30
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 1

Query: 1  MAEIGECSASNSSMENWAKLIPTDKMYADIEINSDETVIFSETKSTSVEKHEWCKITRNS 60
          MAEIGECSASNSS+ENWAKLIPTDKMY DIEINSDETVIFSETKSTSVEKHEWCKITRNS
Sbjct: 1  MAEIGECSASNSSLENWAKLIPTDKMYVDIEINSDETVIFSETKSTSVEKHEWCKITRNS 60

Query: 61 DLNTATLQNR 71
          DLNTATLQNR
Sbjct: 61 DLNTATLQNR 70

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KYR0_CUCSA2.4e-31100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G508530 PE=4 SV=1[more]
A0A067F5J8_CITSI7.4e-1762.86Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g019461mg PE=4 SV=1[more]
B9N5A6_POPTR1.4e-1561.97Zinc finger family protein OS=Populus trichocarpa GN=POPTR_0004s14500g PE=4 SV=1[more]
V4SGG1_9ROSI3.1e-1561.19Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024761mg PE=4 SV=1[more]
A0A0S3QY52_PHAAN1.7e-1354.79Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G065300 PE=... [more]
Match NameE-valueIdentityDescription
AT1G47570.15.8e-1348.57 RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778695005|ref|XP_011653909.1|3.4e-31100.00PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis sativus][more]
gi|700199667|gb|KGN54825.1|3.4e-31100.00hypothetical protein Csa_4G508530 [Cucumis sativus][more]
gi|778695009|ref|XP_004146401.2|3.4e-31100.00PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X2 [Cucumis sativus][more]
gi|778695012|ref|XP_011653910.1|3.4e-31100.00PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X3 [Cucumis sativus][more]
gi|659082892|ref|XP_008442085.1|2.2e-3097.14PREDICTED: E3 ubiquitin-protein ligase CHFR isoform X1 [Cucumis melo][more]
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008270 zinc ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI04G20130CSPI04G20130gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI04G20130.1CSPI04G20130.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G20130.1.cds3CSPI04G20130.1.cds3CDS
CSPI04G20130.1.cds2CSPI04G20130.1.cds2CDS
CSPI04G20130.1.cds1CSPI04G20130.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G20130.1.utr5p1CSPI04G20130.1.utr5p1five_prime_UTR