CSPI04G10650.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G10650.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Gamma-tubulin complex component
Location	Chr4 : 8890607 .. 8891011 (-)
Sequence length	405

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGGCGAATGAAATTTTGAAGAATAAAAGAATTGAAGATATCAGTTGGCTTTGTTCACTATCTGAATCAGAACTTGACCTGCTGATTAGCATTAAAATGCTAGTTCTCCAACGTGCAAAAGCAGTTGGTCATGAAAATTTAGCTGAGAAATTTGATTTGAAGATGCAACGAGCTATTGGGTTTGTTTTGATGGAACATCTTAAAGGAGAACTTAGGACATCGGATGTTTCGGATCTGTCCCAATCTGCCTTAAATGCGTGCAACTTGTTAGATAGCAATCTTGAGAAAATTTTGTCAATTGATGAGATAATGGCGAGTATCTGCTCGGACAGAAGGAAGAAACCAGGAAAAAGGCCACGGGAAAAGGTTGATTCGAGATACAAGAACGACAAGTTGTGA

mRNA sequence

ATGGAAGAGGCGAATGAAATTTTGAAGAATAAAAGAATTGAAGATATCAGTTGGCTTTGTTCACTATCTGAATCAGAACTTGACCTGCTGATTAGCATTAAAATGCTAGTTCTCCAACGTGCAAAAGCAGTTGGTCATGAAAATTTAGCTGAGAAATTTGATTTGAAGATGCAACGAGCTATTGGGTTTGTTTTGATGGAACATCTTAAAGGAGAACTTAGGACATCGGATGTTTCGGATCTGTCCCAATCTGCCTTAAATGCGTGCAACTTGTTAGATAGCAATCTTGAGAAAATTTTGTCAATTGATGAGATAATGGCGAGTATCTGCTCGGACAGAAGGAAGAAACCAGGAAAAAGGCCACGGGAAAAGGTTGATTCGAGATACAAGAACGACAAGTTGTGA

Coding sequence (CDS)

ATGGAAGAGGCGAATGAAATTTTGAAGAATAAAAGAATTGAAGATATCAGTTGGCTTTGTTCACTATCTGAATCAGAACTTGACCTGCTGATTAGCATTAAAATGCTAGTTCTCCAACGTGCAAAAGCAGTTGGTCATGAAAATTTAGCTGAGAAATTTGATTTGAAGATGCAACGAGCTATTGGGTTTGTTTTGATGGAACATCTTAAAGGAGAACTTAGGACATCGGATGTTTCGGATCTGTCCCAATCTGCCTTAAATGCGTGCAACTTGTTAGATAGCAATCTTGAGAAAATTTTGTCAATTGATGAGATAATGGCGAGTATCTGCTCGGACAGAAGGAAGAAACCAGGAAAAAGGCCACGGGAAAAGGTTGATTCGAGATACAAGAACGACAAGTTGTGA

Homology

[Close]

BLAST of CSPI04G10650.1 vs. TrEMBL
Match: A0A0A0KW48_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G166940 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.4e-62
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA
Sbjct: 180 MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 239

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 240 IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 299

Query: 121 PREKVDSRY-KNDKL 135
           P+EKVDSRY KNDKL
Sbjct: 300 PQEKVDSRYKKNDKL 314

[Close]

BLAST of CSPI04G10650.1 vs. TrEMBL
Match: A0A0A0LBP9_CUCSA (Gamma-tubulin complex component OS=Cucumis sativus GN=Csa_3G633280 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.0e-56
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDL+  RA
Sbjct: 59  MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLRTLRA 118

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKG+LRTSDVSDLSQS  NACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 119 IGFVLMEHLKGKLRTSDVSDLSQSTFNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 178

Query: 121 PREKVDSRYKND 133
            REKVD  YK +
Sbjct: 179 SREKVDLSYKKN 190

[Close]

BLAST of CSPI04G10650.1 vs. TrEMBL
Match: A0A061GK54_THECC (Gamma-tubulin complex component OS=Theobroma cacao GN=TCM_029316 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.1e-26
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 1


Query: 2   EEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRAI 61
           E+  E+L N  IED+SWLCSLSESELDLLIS+KML ++RA+ +GH  LA+ FDLKM RA+
Sbjct: 3   EKEEEVLGNNSIEDVSWLCSLSESELDLLISLKMLAMKRARVIGHVQLAKNFDLKMLRAL 62

Query: 62  GFVLMEHLKGELRTSD-VSDLSQSA--LNACNLLDSNLEKILSIDEIMASICSDRRKKPG 121
           GF+LME+LK +++ S  VS +++SA  +++ NLL S L+ ++SI+E+   I    RK+  
Sbjct: 63  GFILMEYLKEKVKDSSIVSGVAESAAFIDSSNLLKSKLDDMMSIEELKEGIAIKTRKEFC 122

Query: 122 KRPREK 125
           KRPREK
Sbjct: 123 KRPREK 128

[Close]

BLAST of CSPI04G10650.1 vs. TrEMBL
Match: A0A061GD22_THECC (Gamma-tubulin complex component (Fragment) OS=Theobroma cacao GN=TCM_029316 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.1e-26
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 1


Query: 2   EEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRAI 61
           E+  E+L N  IED+SWLCSLSESELDLLIS+KML ++RA+ +GH  LA+ FDLKM RA+
Sbjct: 30  EKEEEVLGNNSIEDVSWLCSLSESELDLLISLKMLAMKRARVIGHVQLAKNFDLKMLRAL 89

Query: 62  GFVLMEHLKGELRTSD-VSDLSQSA--LNACNLLDSNLEKILSIDEIMASICSDRRKKPG 121
           GF+LME+LK +++ S  VS +++SA  +++ NLL S L+ ++SI+E+   I    RK+  
Sbjct: 90  GFILMEYLKEKVKDSSIVSGVAESAAFIDSSNLLKSKLDDMMSIEELKEGIAIKTRKEFC 149

Query: 122 KRPREK 125
           KRPREK
Sbjct: 150 KRPREK 155

[Close]

BLAST of CSPI04G10650.1 vs. TrEMBL
Match: A0A0V0HHC0_SOLCH (Gamma-tubulin complex component OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.3e-25
Identity = 66/123 (53.66%), Postives = 94/123 (76.42%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           M+E   IL N RIED+ WLCSL+ESELDLLI +K+L+ QRAK +GH++LA KFDLK  RA
Sbjct: 1   MKEQMAILGNNRIEDVRWLCSLTESELDLLIGLKVLIQQRAKKIGHKSLANKFDLKTLRA 60

Query: 61  IGFVLMEHLKGELR----TSDVSDLSQSALNACNLLDSNLEKI---LSIDEIMASICSDR 117
           + FVLME+LKG+LR    T D ++ S S+L+ACNLL+ +L+K    + ++++ + IC+D+
Sbjct: 61  LSFVLMENLKGKLRDLSGTPDTAE-SSSSLDACNLLNYDLDKTFANMGVEQLSSYICNDK 120

[Close]

BLAST of CSPI04G10650.1 vs. TAIR10
Match: AT1G80245.1 (AT1G80245.1 Spc97 / Spc98 family of spindle pole body (SBP) component)

HSP 1 Score: 115.5 bits (288), Expect = 2.5e-26
Identity = 61/128 (47.66%), Postives = 88/128 (68.75%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEE  +ILKN R +D++W CSLSESELDLLIS+K L +QRAK  GH+ LA+KFDLK+ R+
Sbjct: 1   MEELTQILKNNRTDDLTWFCSLSESELDLLISLKKLAIQRAKISGHQELADKFDLKLLRS 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSA------LNACNLLDSNLEKILSIDEIMASICSDRR 120
           +G VLMEH +   R  + + L+ S       L+ CNLL ++++  + I+EI+  IC ++ 
Sbjct: 61  LGLVLMEHAR--KRVQNDTSLAPSVVHQLRLLDNCNLLKTHVDDAVDIEEILTQICDNKS 120

Query: 121 KKPGKRPR 123
           KK  ++ R
Sbjct: 121 KKKARKRR 126

[Close]

BLAST of CSPI04G10650.1 vs. TAIR10
Match: AT4G00695.3 (AT4G00695.3 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 107.1 bits (266), Expect = 8.8e-24
Identity = 61/127 (48.03%), Postives = 88/127 (69.29%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENL-AEKFDLKMQR 60
           MEE ++ILKN R +D++W CSLSESELDLLIS+K LV++RAK  G E+L A+KFDLKM R
Sbjct: 1   MEELSQILKNNRTDDLTWFCSLSESELDLLISLKKLVIRRAKVSGLEDLVADKFDLKMLR 60

Query: 61  AIGFVLMEHLKGELRTSDVSDLSQSA------LNACNLLDSNLEKILSIDEIMASICSDR 120
           ++G VLME+++   R  D + L+ S       L++CNLL ++++  + I+EI+  IC+  
Sbjct: 61  SLGLVLMEYVRK--RVEDDTSLAPSVVQELSLLDSCNLLKTHVDDTVDIEEILTDICNKN 120

[Close]

BLAST of CSPI04G10650.1 vs. NCBI nr
Match: gi|778692248|ref|XP_011653427.1| (PREDICTED: uncharacterized protein LOC105435221 [Cucumis sativus])

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-62
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA
Sbjct: 1   MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120

Query: 121 PREKVDSRY-KNDKL 135
           P+EKVDSRY KNDKL
Sbjct: 121 PQEKVDSRYKKNDKL 135

[Close]

BLAST of CSPI04G10650.1 vs. NCBI nr
Match: gi|700198692|gb|KGN53850.1| (hypothetical protein Csa_4G166940 [Cucumis sativus])

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-62
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA
Sbjct: 180 MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 239

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 240 IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 299

Query: 121 PREKVDSRY-KNDKL 135
           P+EKVDSRY KNDKL
Sbjct: 300 PQEKVDSRYKKNDKL 314

[Close]

BLAST of CSPI04G10650.1 vs. NCBI nr
Match: gi|778682197|ref|XP_011651668.1| (PREDICTED: uncharacterized protein LOC101210446 isoform X2 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 2.8e-56
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDL+  RA
Sbjct: 1   MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLRTLRA 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKG+LRTSDVSDLSQS  NACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 61  IGFVLMEHLKGKLRTSDVSDLSQSTFNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120

Query: 121 PREKVDSRYKND 133
            REKVD  YK +
Sbjct: 121 SREKVDLSYKKN 132

[Close]

BLAST of CSPI04G10650.1 vs. NCBI nr
Match: gi|700203241|gb|KGN58374.1| (hypothetical protein Csa_3G633280 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 2.8e-56
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDL+  RA
Sbjct: 59  MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLRTLRA 118

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKG+LRTSDVSDLSQS  NACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 119 IGFVLMEHLKGKLRTSDVSDLSQSTFNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 178

Query: 121 PREKVDSRYKND 133
            REKVD  YK +
Sbjct: 179 SREKVDLSYKKN 190

[Close]

BLAST of CSPI04G10650.1 vs. NCBI nr
Match: gi|659120858|ref|XP_008460383.1| (PREDICTED: uncharacterized protein LOC103499220 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 5.3e-55
Identity = 116/126 (92.06%), Postives = 118/126 (93.65%), Query Frame = 1


Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKN RIEDISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDLK  R 
Sbjct: 1   MEEANEILKNNRIEDISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLKTLRT 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDV+DLSQSALNACNLLDSNLEK L IDEIM SIC DRRKKPGKR
Sbjct: 61  IGFVLMEHLKGELRTSDVTDLSQSALNACNLLDSNLEKSLPIDEIMTSICLDRRKKPGKR 120

Query: 121 PREKVD 127
           PREKVD
Sbjct: 121 PREKVD 126

The following BLAST results are available for this feature:

BLAST of CSPI04G10650.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI04G10650.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW48_CUCSA	1.4e-62	97.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G166940 PE=4 SV=1	[more]
A0A0A0LBP9_CUCSA	2.0e-56	90.15	Gamma-tubulin complex component OS=Cucumis sativus GN=Csa_3G633280 PE=3 SV=1	[more]
A0A061GK54_THECC	8.1e-26	53.97	Gamma-tubulin complex component OS=Theobroma cacao GN=TCM_029316 PE=3 SV=1	[more]
A0A061GD22_THECC	8.1e-26	53.97	Gamma-tubulin complex component (Fragment) OS=Theobroma cacao GN=TCM_029316 PE=3...	[more]
A0A0V0HHC0_SOLCH	2.3e-25	53.66	Gamma-tubulin complex component OS=Solanum chacoense PE=3 SV=1	[more]

BLAST of CSPI04G10650.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G80245.1	2.5e-26	47.66	Spc97 / Spc98 family of spindle pole body (SBP) component	[more]
AT4G00695.3	8.8e-24	48.03	FUNCTIONS IN: molecular_function unknown	[more]

BLAST of CSPI04G10650.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778692248\|ref\|XP_011653427.1\|	2.0e-62	97.78	PREDICTED: uncharacterized protein LOC105435221 [Cucumis sativus]	[more]
gi\|700198692\|gb\|KGN53850.1\|	2.0e-62	97.78	hypothetical protein Csa_4G166940 [Cucumis sativus]	[more]
gi\|778682197\|ref\|XP_011651668.1\|	2.8e-56	90.15	PREDICTED: uncharacterized protein LOC101210446 isoform X2 [Cucumis sativus]	[more]
gi\|700203241\|gb\|KGN58374.1\|	2.8e-56	90.15	hypothetical protein Csa_3G633280 [Cucumis sativus]	[more]
gi\|659120858\|ref\|XP_008460383.1\|	5.3e-55	92.06	PREDICTED: uncharacterized protein LOC103499220 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR007259	GCP

Vocabulary: **Biological Process**
Term	Definition
GO:0000226	microtubule cytoskeleton organization
GO:0007020	microtubule nucleation

Vocabulary: **Cellular Component**
Term	Definition
GO:0000922	spindle pole
GO:0005815	microtubule organizing center

Vocabulary: **Molecular Function**
Term	Definition
GO:0043015	gamma-tubulin binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000226	microtubule cytoskeleton organization
biological_process	GO:0007020	microtubule nucleation
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0006412	translation
cellular_component	GO:0005815	microtubule organizing center
cellular_component	GO:0000922	spindle pole
cellular_component	GO:0005840	ribosome
cellular_component	GO:0045298	tubulin complex
molecular_function	GO:0043015	gamma-tubulin binding
molecular_function	GO:0003735	structural constituent of ribosome

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G10650	CSPI04G10650	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G10650.1	CSPI04G10650.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G10650.1.cds1	CSPI04G10650.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007259	Gamma-tubulin complex component protein	PANTHER	PTHR19302	GAMMA TUBULIN COMPLEX PROTEIN	coord: 26..115 score: 1.6
None	No IPR available	PANTHER	PTHR19302:SF39	SUBFAMILY NOT NAMED	coord: 26..115 score: 1.6