CSPI04G10650 (gene) Wild cucumber (PI 183967)

NameCSPI04G10650
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionGamma-tubulin complex component
LocationChr4 : 8890607 .. 8891011 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAGGCGAATGAAATTTTGAAGAATAAAAGAATTGAAGATATCAGTTGGCTTTGTTCACTATCTGAATCAGAACTTGACCTGCTGATTAGCATTAAAATGCTAGTTCTCCAACGTGCAAAAGCAGTTGGTCATGAAAATTTAGCTGAGAAATTTGATTTGAAGATGCAACGAGCTATTGGGTTTGTTTTGATGGAACATCTTAAAGGAGAACTTAGGACATCGGATGTTTCGGATCTGTCCCAATCTGCCTTAAATGCGTGCAACTTGTTAGATAGCAATCTTGAGAAAATTTTGTCAATTGATGAGATAATGGCGAGTATCTGCTCGGACAGAAGGAAGAAACCAGGAAAAAGGCCACGGGAAAAGGTTGATTCGAGATACAAGAACGACAAGTTGTGA

mRNA sequence

ATGGAAGAGGCGAATGAAATTTTGAAGAATAAAAGAATTGAAGATATCAGTTGGCTTTGTTCACTATCTGAATCAGAACTTGACCTGCTGATTAGCATTAAAATGCTAGTTCTCCAACGTGCAAAAGCAGTTGGTCATGAAAATTTAGCTGAGAAATTTGATTTGAAGATGCAACGAGCTATTGGGTTTGTTTTGATGGAACATCTTAAAGGAGAACTTAGGACATCGGATGTTTCGGATCTGTCCCAATCTGCCTTAAATGCGTGCAACTTGTTAGATAGCAATCTTGAGAAAATTTTGTCAATTGATGAGATAATGGCGAGTATCTGCTCGGACAGAAGGAAGAAACCAGGAAAAAGGCCACGGGAAAAGGTTGATTCGAGATACAAGAACGACAAGTTGTGA

Coding sequence (CDS)

ATGGAAGAGGCGAATGAAATTTTGAAGAATAAAAGAATTGAAGATATCAGTTGGCTTTGTTCACTATCTGAATCAGAACTTGACCTGCTGATTAGCATTAAAATGCTAGTTCTCCAACGTGCAAAAGCAGTTGGTCATGAAAATTTAGCTGAGAAATTTGATTTGAAGATGCAACGAGCTATTGGGTTTGTTTTGATGGAACATCTTAAAGGAGAACTTAGGACATCGGATGTTTCGGATCTGTCCCAATCTGCCTTAAATGCGTGCAACTTGTTAGATAGCAATCTTGAGAAAATTTTGTCAATTGATGAGATAATGGCGAGTATCTGCTCGGACAGAAGGAAGAAACCAGGAAAAAGGCCACGGGAAAAGGTTGATTCGAGATACAAGAACGACAAGTTGTGA
BLAST of CSPI04G10650 vs. TrEMBL
Match: A0A0A0KW48_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G166940 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.4e-62
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA
Sbjct: 180 MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 239

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 240 IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 299

Query: 121 PREKVDSRY-KNDKL 135
           P+EKVDSRY KNDKL
Sbjct: 300 PQEKVDSRYKKNDKL 314

BLAST of CSPI04G10650 vs. TrEMBL
Match: A0A0A0LBP9_CUCSA (Gamma-tubulin complex component OS=Cucumis sativus GN=Csa_3G633280 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.0e-56
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDL+  RA
Sbjct: 59  MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLRTLRA 118

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKG+LRTSDVSDLSQS  NACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 119 IGFVLMEHLKGKLRTSDVSDLSQSTFNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 178

Query: 121 PREKVDSRYKND 133
            REKVD  YK +
Sbjct: 179 SREKVDLSYKKN 190

BLAST of CSPI04G10650 vs. TrEMBL
Match: A0A061GK54_THECC (Gamma-tubulin complex component OS=Theobroma cacao GN=TCM_029316 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.1e-26
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 1

Query: 2   EEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRAI 61
           E+  E+L N  IED+SWLCSLSESELDLLIS+KML ++RA+ +GH  LA+ FDLKM RA+
Sbjct: 3   EKEEEVLGNNSIEDVSWLCSLSESELDLLISLKMLAMKRARVIGHVQLAKNFDLKMLRAL 62

Query: 62  GFVLMEHLKGELRTSD-VSDLSQSA--LNACNLLDSNLEKILSIDEIMASICSDRRKKPG 121
           GF+LME+LK +++ S  VS +++SA  +++ NLL S L+ ++SI+E+   I    RK+  
Sbjct: 63  GFILMEYLKEKVKDSSIVSGVAESAAFIDSSNLLKSKLDDMMSIEELKEGIAIKTRKEFC 122

Query: 122 KRPREK 125
           KRPREK
Sbjct: 123 KRPREK 128

BLAST of CSPI04G10650 vs. TrEMBL
Match: A0A061GD22_THECC (Gamma-tubulin complex component (Fragment) OS=Theobroma cacao GN=TCM_029316 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 8.1e-26
Identity = 68/126 (53.97%), Postives = 95/126 (75.40%), Query Frame = 1

Query: 2   EEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRAI 61
           E+  E+L N  IED+SWLCSLSESELDLLIS+KML ++RA+ +GH  LA+ FDLKM RA+
Sbjct: 30  EKEEEVLGNNSIEDVSWLCSLSESELDLLISLKMLAMKRARVIGHVQLAKNFDLKMLRAL 89

Query: 62  GFVLMEHLKGELRTSD-VSDLSQSA--LNACNLLDSNLEKILSIDEIMASICSDRRKKPG 121
           GF+LME+LK +++ S  VS +++SA  +++ NLL S L+ ++SI+E+   I    RK+  
Sbjct: 90  GFILMEYLKEKVKDSSIVSGVAESAAFIDSSNLLKSKLDDMMSIEELKEGIAIKTRKEFC 149

Query: 122 KRPREK 125
           KRPREK
Sbjct: 150 KRPREK 155

BLAST of CSPI04G10650 vs. TrEMBL
Match: A0A0V0HHC0_SOLCH (Gamma-tubulin complex component OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.3e-25
Identity = 66/123 (53.66%), Postives = 94/123 (76.42%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           M+E   IL N RIED+ WLCSL+ESELDLLI +K+L+ QRAK +GH++LA KFDLK  RA
Sbjct: 1   MKEQMAILGNNRIEDVRWLCSLTESELDLLIGLKVLIQQRAKKIGHKSLANKFDLKTLRA 60

Query: 61  IGFVLMEHLKGELR----TSDVSDLSQSALNACNLLDSNLEKI---LSIDEIMASICSDR 117
           + FVLME+LKG+LR    T D ++ S S+L+ACNLL+ +L+K    + ++++ + IC+D+
Sbjct: 61  LSFVLMENLKGKLRDLSGTPDTAE-SSSSLDACNLLNYDLDKTFANMGVEQLSSYICNDK 120

BLAST of CSPI04G10650 vs. TAIR10
Match: AT1G80245.1 (AT1G80245.1 Spc97 / Spc98 family of spindle pole body (SBP) component)

HSP 1 Score: 115.5 bits (288), Expect = 2.5e-26
Identity = 61/128 (47.66%), Postives = 88/128 (68.75%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEE  +ILKN R +D++W CSLSESELDLLIS+K L +QRAK  GH+ LA+KFDLK+ R+
Sbjct: 1   MEELTQILKNNRTDDLTWFCSLSESELDLLISLKKLAIQRAKISGHQELADKFDLKLLRS 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSA------LNACNLLDSNLEKILSIDEIMASICSDRR 120
           +G VLMEH +   R  + + L+ S       L+ CNLL ++++  + I+EI+  IC ++ 
Sbjct: 61  LGLVLMEHAR--KRVQNDTSLAPSVVHQLRLLDNCNLLKTHVDDAVDIEEILTQICDNKS 120

Query: 121 KKPGKRPR 123
           KK  ++ R
Sbjct: 121 KKKARKRR 126

BLAST of CSPI04G10650 vs. TAIR10
Match: AT4G00695.3 (AT4G00695.3 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 107.1 bits (266), Expect = 8.8e-24
Identity = 61/127 (48.03%), Postives = 88/127 (69.29%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENL-AEKFDLKMQR 60
           MEE ++ILKN R +D++W CSLSESELDLLIS+K LV++RAK  G E+L A+KFDLKM R
Sbjct: 1   MEELSQILKNNRTDDLTWFCSLSESELDLLISLKKLVIRRAKVSGLEDLVADKFDLKMLR 60

Query: 61  AIGFVLMEHLKGELRTSDVSDLSQSA------LNACNLLDSNLEKILSIDEIMASICSDR 120
           ++G VLME+++   R  D + L+ S       L++CNLL ++++  + I+EI+  IC+  
Sbjct: 61  SLGLVLMEYVRK--RVEDDTSLAPSVVQELSLLDSCNLLKTHVDDTVDIEEILTDICNKN 120

BLAST of CSPI04G10650 vs. NCBI nr
Match: gi|778692248|ref|XP_011653427.1| (PREDICTED: uncharacterized protein LOC105435221 [Cucumis sativus])

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-62
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA
Sbjct: 1   MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120

Query: 121 PREKVDSRY-KNDKL 135
           P+EKVDSRY KNDKL
Sbjct: 121 PQEKVDSRYKKNDKL 135

BLAST of CSPI04G10650 vs. NCBI nr
Match: gi|700198692|gb|KGN53850.1| (hypothetical protein Csa_4G166940 [Cucumis sativus])

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-62
Identity = 132/135 (97.78%), Postives = 133/135 (98.52%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA
Sbjct: 180 MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 239

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 240 IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 299

Query: 121 PREKVDSRY-KNDKL 135
           P+EKVDSRY KNDKL
Sbjct: 300 PQEKVDSRYKKNDKL 314

BLAST of CSPI04G10650 vs. NCBI nr
Match: gi|778682197|ref|XP_011651668.1| (PREDICTED: uncharacterized protein LOC101210446 isoform X2 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 2.8e-56
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDL+  RA
Sbjct: 1   MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLRTLRA 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKG+LRTSDVSDLSQS  NACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 61  IGFVLMEHLKGKLRTSDVSDLSQSTFNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120

Query: 121 PREKVDSRYKND 133
            REKVD  YK +
Sbjct: 121 SREKVDLSYKKN 132

BLAST of CSPI04G10650 vs. NCBI nr
Match: gi|700203241|gb|KGN58374.1| (hypothetical protein Csa_3G633280 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 2.8e-56
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKNKRIE ISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDL+  RA
Sbjct: 59  MEEANEILKNKRIEGISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLRTLRA 118

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKG+LRTSDVSDLSQS  NACNLLDSNLEKILSIDEIMASICSDRRKKPGKR
Sbjct: 119 IGFVLMEHLKGKLRTSDVSDLSQSTFNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 178

Query: 121 PREKVDSRYKND 133
            REKVD  YK +
Sbjct: 179 SREKVDLSYKKN 190

BLAST of CSPI04G10650 vs. NCBI nr
Match: gi|659120858|ref|XP_008460383.1| (PREDICTED: uncharacterized protein LOC103499220 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 5.3e-55
Identity = 116/126 (92.06%), Postives = 118/126 (93.65%), Query Frame = 1

Query: 1   MEEANEILKNKRIEDISWLCSLSESELDLLISIKMLVLQRAKAVGHENLAEKFDLKMQRA 60
           MEEANEILKN RIEDISWLCSLSESELDLLISIKMLVLQRAKA+GHENLAEKFDLK  R 
Sbjct: 1   MEEANEILKNNRIEDISWLCSLSESELDLLISIKMLVLQRAKAIGHENLAEKFDLKTLRT 60

Query: 61  IGFVLMEHLKGELRTSDVSDLSQSALNACNLLDSNLEKILSIDEIMASICSDRRKKPGKR 120
           IGFVLMEHLKGELRTSDV+DLSQSALNACNLLDSNLEK L IDEIM SIC DRRKKPGKR
Sbjct: 61  IGFVLMEHLKGELRTSDVTDLSQSALNACNLLDSNLEKSLPIDEIMTSICLDRRKKPGKR 120

Query: 121 PREKVD 127
           PREKVD
Sbjct: 121 PREKVD 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KW48_CUCSA1.4e-6297.78Uncharacterized protein OS=Cucumis sativus GN=Csa_4G166940 PE=4 SV=1[more]
A0A0A0LBP9_CUCSA2.0e-5690.15Gamma-tubulin complex component OS=Cucumis sativus GN=Csa_3G633280 PE=3 SV=1[more]
A0A061GK54_THECC8.1e-2653.97Gamma-tubulin complex component OS=Theobroma cacao GN=TCM_029316 PE=3 SV=1[more]
A0A061GD22_THECC8.1e-2653.97Gamma-tubulin complex component (Fragment) OS=Theobroma cacao GN=TCM_029316 PE=3... [more]
A0A0V0HHC0_SOLCH2.3e-2553.66Gamma-tubulin complex component OS=Solanum chacoense PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G80245.12.5e-2647.66 Spc97 / Spc98 family of spindle pole body (SBP) component[more]
AT4G00695.38.8e-2448.03 FUNCTIONS IN: molecular_function unknown[more]
Match NameE-valueIdentityDescription
gi|778692248|ref|XP_011653427.1|2.0e-6297.78PREDICTED: uncharacterized protein LOC105435221 [Cucumis sativus][more]
gi|700198692|gb|KGN53850.1|2.0e-6297.78hypothetical protein Csa_4G166940 [Cucumis sativus][more]
gi|778682197|ref|XP_011651668.1|2.8e-5690.15PREDICTED: uncharacterized protein LOC101210446 isoform X2 [Cucumis sativus][more]
gi|700203241|gb|KGN58374.1|2.8e-5690.15hypothetical protein Csa_3G633280 [Cucumis sativus][more]
gi|659120858|ref|XP_008460383.1|5.3e-5592.06PREDICTED: uncharacterized protein LOC103499220 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR007259GCP
Vocabulary: Biological Process
TermDefinition
GO:0000226microtubule cytoskeleton organization
GO:0007020microtubule nucleation
Vocabulary: Cellular Component
TermDefinition
GO:0000922spindle pole
GO:0005815microtubule organizing center
Vocabulary: Molecular Function
TermDefinition
GO:0043015gamma-tubulin binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007020 microtubule nucleation
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0006412 translation
biological_process GO:0000226 microtubule cytoskeleton organization
cellular_component GO:0005815 microtubule organizing center
cellular_component GO:0005840 ribosome
cellular_component GO:0000922 spindle pole
cellular_component GO:0045298 tubulin complex
molecular_function GO:0043015 gamma-tubulin binding
molecular_function GO:0003735 structural constituent of ribosome

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G10650.1CSPI04G10650.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007259Gamma-tubulin complex component proteinPANTHERPTHR19302GAMMA TUBULIN COMPLEX PROTEINcoord: 26..115
score: 1.6
NoneNo IPR availablePANTHERPTHR19302:SF39SUBFAMILY NOT NAMEDcoord: 26..115
score: 1.6