CSPI04G04390.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G04390.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	DNA-directed RNA polymerase subunit beta
Location	Chr4 : 2854765 .. 2858253 (+)
Sequence length	657

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAATCAAAAGAAAACATTTCAAATACATTTGTTGAAATATTTAATTCCAAGCAAAGACTTATTGAAGAAGGAAAAATTAGAAAACGAAGTATTTTTTATGTTTCTCTTAATTTGAACTCAAAGGTTAGTTTCCAGTTTGACGATCTGTCAGCAAGTCTTAATGATAATTCCTATTGGCGCAGTGATCAAAGGTAAAACGCCGTCGTTTATTGTCCACCGCCACATCTTCCTCGCTACTACCATCCAAATTCCAAGTACTCTATGTCCTTCTTTCTTCATTCATAGTACGCAACGTCAAAATTTCAGCGCCTGTCATTTTCCCCTCAACTAATTTCCTCTACTCTCTTCCTAGTTTCTTCTCCTTCCCCGCCATTGAAATACTCGTCCTTAATACCAACCTCCACCCGAAACTCTCATCCTTCCTCTTTCCTTTTTTTTTTTTTTAATTCCAGTTAGTACTCGCCAGAGATGGAGAGGAAGTCGGCTCTGATGGTGGAATATGTGGTTGCTATTTTAGGGATTGTGATGATCGCCACTGGCTTCGCCGCTGAAGCCACAAGAACTAAGGTGAAGATTCAATTAATGATTACTGTTTGATCGCGGGGTTTTTTTTCGCCAGAGATGGAGAGGAAGTCGGCTCTGATGGTGGAATATGTGGTTGCTATTTTAGGGATTGTGATGATCGCCACTGGCTTCGCCGCTGAAGCCACAAGAACTAAGGTGAAGATTCAATTAATGATTACTGTTTGACCGCGGGGTTTTTTTTTTTTTTTTTTTTTTTCTGTTTGGCTGATGAGATATTGTGTGGGAAAGAATATGGAAGAACAGTTTTGAAGTAGGGTTCGTTTTAAATTGGGTATGTAATATGGTTGAATTTGATTAAAAATTATATAGAAAGAAGGTGGTGGTGAATTTTAAATACAGTTTGATTTGGATCTGATTTTCTTTTTTGTTTTGGCTTCTTCTTTTTATCATTACTTGTTTCCCAAGCCCTGATCTACAGGTTAATTGATTAGACTGATGGATTGGGGGTGTTATTATATCTAATCATACTTTTTTTTTTTTTTTTAATTTTCTGTTTTCTATTTTTGTGTGTGCGTGTGTGTGCTTTTAACTTTTTAGTATAGTAAAACTTGAGAATGATGATTTGAATCAGATGGAACGGTACTTTTCCTCTAATTTCAAGTAATTTGTAGGAGTTGGTTTGATAATGATTTTGTTTTTAATTTTCCATTTCTAGAGTTGACAAATTTGGTGAAAGTTTATTTTGTTGAGTGTTAAAATTTAGTTGGTCTCTGAAACTCACATATCGACCTAATATCTAATTTGGTTCGTACAATTTCGAAGAAATTTTCATTTTAATCCCTTGAGTTTTTACAAGTTACCGGGTTCTGTTCATGATGCCTTTGACGATACATCTGATGAAACAGAGAAAGTGCTCTGGATCAAGGAAATAATAGTTCAACTAGGGCAGATTAATAATCATCTAATGAAATTAGATCTGATTAGAACATGCTTGAGAGTGATTTTAAAACAACCACACTTTTTATCTTTCAAAATCAGTTTAATGTTTGCTTTTAAACTTTGACCAGGGATTTTCATACAATCAAATTTTAGAAGCACTTTTAATAAGCATGCCCTTGTTGGTTTTGAGACTTATGATTACGAGTCCACACTTTTTGATCACTATAAGAGAATTTTTCACTTTAAACGAAATTTCTATGCTAACCCAAATCATCATTGGCTTCTTTCTCTTTCCCTTGATATATGTTGATTTTGGTAACAGTTCTTTTGAGTTTAACGTGGGATTGATGCATTTTAAAGTTTGACACATCAATTTTGTTCATTTGACAGGGGAATCAAGTCACTAAAGTTGCTCCTGATTTGTGTAAATATCCCCGAAGTCCAGCATTGGGCCTTGGTTTGACAGCCGCACTATCGCTTTTGTTTGCTCAAATAACAATAAAGGCTTCGACGGGATGCGTTTGCTGCATTAGAGGTCCTCGGCCTCCTGCTTCTAAATGGAGAACAGCTGTGATCTGCTTTACCATTTCGTGGTATTTTTCGACCTTTCCTTTCTCATTCTAATTTGCATTTAGTTTATGGTTTGCTGTGTTTCTGTTGTATCATGAGATGAATTATAGGAAAATCAATTGAAACCAGAAGAGTTCTTGCGATTTGGTTTTCTCAAGTATAGGAAGAAATTTTGAACTCGCCTAGTCGAAATAAAGGTTATTTTATTGTGATCGATTAATCAATCTTCTGCCCCGGTTCTTGATGGCCCAACTTTACGTTACGATATTGTTTGTGATAATATAATTTTCTTTTTTTCTGCTTTAAAAACAGTTCCATGTTCTTGTTATCATCTTTTTGGCTTTTTCTGCCTTGTTAAGTCGTTATGATTTTTGTTACTCTTGTTTTTATCGAACATCTTAACCAAGAGAAGAGACTTCCTGATGTGACCTGATCCATGATCATCATAGTGTAAATGATCGATCTATTGTTTTGGATAAACGTTCTACTTTAATTTTCAACTAAAAGAACAATCGATGCCTTCAATTCTGAATGAAAATAGAACAAACGTAGATTCTCATTGTCCTCTGTTTTAGTCCCATCATCACGCCTTTGTCAACCATCATGGGTTTAGTTATTAGTAAATAAAAAACATGCGTTTGAGAAAAGGTCAAGATTGAAATGTGTTCAATCCATGGTGATCACATATTTAGAATTTAATGAGTTCTCTGCGGTTGTTTCATAAGGTTAGTTGAGGTGTCCATACTAACAAATATGAAGCAAAGACCTATCTTGAGTCTGAATTTTCCTTCCCTTTCCTTGTACCTACACCCTCAGCTTAATATCTTCTGCAGGGTTACATATGTGATAGCATTCCTCCTGTTCCTCACCGGTGCTGCACTGAACAACGGACGGGGCGAACAACGCAACTATTTCCGTGACTACGACTGCTATGTTCTAAAACCTGGAGTTTTTTCTTTTGCCACCATTGTAGGTATGGCAAGTTTAACTCTGGGAATGTCCTACTTCCTCATATTGAACTCTGCAAAGAATGACCCTTCTACTGTGTGGGGCCATCCTTCCGTTCCTCCTCAACCAAACATCGCAATGGCACAACCCCAGTTCCCAAACCCTCCTCCACCACCACAGAGAACTGCCGACCCCGTATTCGTTCACGAAGACACGTACATGAGACGACAATTCACGTGATCGGGAAATGTAGGTTGACACCAAATGCGTTTAAGAAACAACCGTGTAACTCATATCACTTGATTGATGTATCTTTTTAAACCAGGGATTGAAGGTGCACAAATACATTGGCTCTATAGAAAATAGTTGTTTTGGAGCTGTGTAGGCTCTCTTTCATAGAATTGATTTTGTGAAAGAAGGTGTCATTTTATTTCCCTTTGATCCAAAGGGAAGTGAATAGATTGTAATGTAATAAAATGTACATCCTTGATTTGTTGTGGGAAG

mRNA sequence

ATGGAGAGGAAGTCGGCTCTGATGGTGGAATATGTGGTTGCTATTTTAGGGATTGTGATGATCGCCACTGGCTTCGCCGCTGAAGCCACAAGAACTAAGGGGAATCAAGTCACTAAAGTTGCTCCTGATTTGTGTAAATATCCCCGAAGTCCAGCATTGGGCCTTGGTTTGACAGCCGCACTATCGCTTTTGTTTGCTCAAATAACAATAAAGGCTTCGACGGGATGCGTTTGCTGCATTAGAGGTCCTCGGCCTCCTGCTTCTAAATGGAGAACAGCTGTGATCTGCTTTACCATTTCGTGGGTTACATATGTGATAGCATTCCTCCTGTTCCTCACCGGTGCTGCACTGAACAACGGACGGGGCGAACAACGCAACTATTTCCGTGACTACGACTGCTATGTTCTAAAACCTGGAGTTTTTTCTTTTGCCACCATTGTAGGTATGGCAAGTTTAACTCTGGGAATGTCCTACTTCCTCATATTGAACTCTGCAAAGAATGACCCTTCTACTGTGTGGGGCCATCCTTCCGTTCCTCCTCAACCAAACATCGCAATGGCACAACCCCAGTTCCCAAACCCTCCTCCACCACCACAGAGAACTGCCGACCCCGTATTCGTTCACGAAGACACGTACATGAGACGACAATTCACGTGA

Coding sequence (CDS)

ATGGAGAGGAAGTCGGCTCTGATGGTGGAATATGTGGTTGCTATTTTAGGGATTGTGATGATCGCCACTGGCTTCGCCGCTGAAGCCACAAGAACTAAGGGGAATCAAGTCACTAAAGTTGCTCCTGATTTGTGTAAATATCCCCGAAGTCCAGCATTGGGCCTTGGTTTGACAGCCGCACTATCGCTTTTGTTTGCTCAAATAACAATAAAGGCTTCGACGGGATGCGTTTGCTGCATTAGAGGTCCTCGGCCTCCTGCTTCTAAATGGAGAACAGCTGTGATCTGCTTTACCATTTCGTGGGTTACATATGTGATAGCATTCCTCCTGTTCCTCACCGGTGCTGCACTGAACAACGGACGGGGCGAACAACGCAACTATTTCCGTGACTACGACTGCTATGTTCTAAAACCTGGAGTTTTTTCTTTTGCCACCATTGTAGGTATGGCAAGTTTAACTCTGGGAATGTCCTACTTCCTCATATTGAACTCTGCAAAGAATGACCCTTCTACTGTGTGGGGCCATCCTTCCGTTCCTCCTCAACCAAACATCGCAATGGCACAACCCCAGTTCCCAAACCCTCCTCCACCACCACAGAGAACTGCCGACCCCGTATTCGTTCACGAAGACACGTACATGAGACGACAATTCACGTGA

Homology

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BLAST of CSPI04G04390.1 vs. TrEMBL
Match: A0A0A0KU80_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025740 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 7.7e-119
Identity = 216/218 (99.08%), Postives = 217/218 (99.54%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERKSALMV+YVVAILGIVMIATGFAAEATRTK NQVTKVAPDLCKYPRSPALGLGLTAA
Sbjct: 1   MERKSALMVKYVVAILGIVMIATGFAAEATRTKRNQVTKVAPDLCKYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG
Sbjct: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
           RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP
Sbjct: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
           QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT
Sbjct: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 218

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BLAST of CSPI04G04390.1 vs. TrEMBL
Match: A0A067EBD4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g028344mg PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 3.4e-66
Identity = 133/218 (61.01%), Postives = 162/218 (74.31%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK  L +   V +LG++  ATGF AEATR KG+QV   +P  C YPRSPALGLGLTAA
Sbjct: 1   MERK-VLALCSTVGLLGLLSAATGFGAEATRIKGSQVQITSPTQCSYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           LSLL AQ+TI  +TGC+CC RGP P  S W  A++CF +SW T+VIAFLL LTGAALN+ 
Sbjct: 61  LSLLIAQVTINVATGCICCRRGPHPSNSNWTIALVCFVVSWFTFVIAFLLLLTGAALNDQ 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
            GE+  YF +Y CYV+KPGVF+   ++ +AS+TLG+ Y+L L+SAKN  S +WG+ SVP 
Sbjct: 121 HGEESMYFGNYYCYVVKPGVFAGGAVLSLASVTLGILYYLTLHSAKN--SGLWGNSSVPQ 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
           +  IAM QPQF     PPQRT +PVFVHEDTYMRRQFT
Sbjct: 181 EGGIAMGQPQF-----PPQRTQEPVFVHEDTYMRRQFT 210

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BLAST of CSPI04G04390.1 vs. TrEMBL
Match: A0A068U9S1_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00019822001 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-59
Identity = 123/216 (56.94%), Postives = 154/216 (71.30%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK  ++V  VV  LG++  ATGFAAEATR KG+QV   +PD+C YPRSPALGLGLTAA
Sbjct: 1   MERK-VIVVCCVVGFLGLLSAATGFAAEATRIKGDQVKFPSPDVCVYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           ++L+ AQ+ +  +TGC+CC RGP    S W+ A++CF +SW T+V+AFLL LTGAALN+ 
Sbjct: 61  VTLMIAQVIVNVATGCICCRRGPHQANSNWKLALVCFIVSWFTFVVAFLLLLTGAALNDQ 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
            GE+  YF  Y CYV+KPGVF+ A I+ +AS+TLG+ Y+++L S KN     WG  S P 
Sbjct: 121 HGEENMYFNYYYCYVVKPGVFAGAAILSLASVTLGLIYYVMLTSTKN-VIDAWGGSSAPV 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQ 217
              IAM QPQF     P Q T  PVFVHEDTYMRRQ
Sbjct: 181 PGGIAMGQPQF-----PAQNTEAPVFVHEDTYMRRQ 209

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BLAST of CSPI04G04390.1 vs. TrEMBL
Match: D7T7U5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0039g02520 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.6e-58
Identity = 127/224 (56.70%), Postives = 156/224 (69.64%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MER+S ++  +V   LG++  A GFAAEA R KG+QV   +   C YPRSPAL LGLTAA
Sbjct: 1   MERRSLVLCTFV-GFLGLLSAALGFAAEAKRIKGSQVQFSSSTACTYPRSPALPLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           ++L+ AQ+ I  +TGC+CC RGP P  S W  A+ICF +SW T+VIAFLL LTGAALN+ 
Sbjct: 61  VALMIAQVMINIATGCICCRRGPHPSNSNWTLALICFVVSWFTFVIAFLLLLTGAALNDQ 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAK--NDPSTVWGHPSV 180
            GE+  YF +Y CYV+KPGVF+ A ++ +AS+TLG+ Y+L L+SAK  NDP   W  P  
Sbjct: 121 HGEESMYFGNYYCYVVKPGVFAGAAVLSLASVTLGILYYLTLSSAKERNDP---WPGPVA 180

Query: 181 PPQP----NIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
           P Q      IAM QPQFP   PP Q   DPVFVHEDTYMRRQFT
Sbjct: 181 PNQAQVGGGIAMGQPQFPPQNPPNQ---DPVFVHEDTYMRRQFT 217

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BLAST of CSPI04G04390.1 vs. TrEMBL
Match: M5X117_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011406mg PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 9.9e-58
Identity = 123/219 (56.16%), Postives = 153/219 (69.86%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK  L+V   V +LG++  ATGF AE TR KG+QV  V+   C+YPRSPALGLG+TAA
Sbjct: 1   MERK-VLLVCCAVGLLGLLSAATGFGAEVTRIKGSQVRFVSVTQCEYPRSPALGLGVTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           ++L+ AQI +  STGC+CC R P+P  S W  A+ CF +SW T+VIAFLL LTGA LN+ 
Sbjct: 61  VALMLAQIILNVSTGCICCKRSPQPSNSNWTVALFCFVVSWFTFVIAFLLLLTGATLNDR 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
            G +  YF +Y CYV+KPGVF     + +AS+ LG+ Y++ LNS K D ++ WG  S PP
Sbjct: 121 HGVESMYFGNYYCYVVKPGVFGGGAGLSLASVVLGIVYYVTLNSVK-DSNSPWG-TSAPP 180

Query: 181 QPN-IAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
            P  IAM QPQF    PPP  T +PVFVHEDTYMRRQFT
Sbjct: 181 NPGAIAMGQPQF----PPPSTTQEPVFVHEDTYMRRQFT 212

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BLAST of CSPI04G04390.1 vs. TAIR10
Match: AT5G17210.1 (AT5G17210.1 Protein of unknown function (DUF1218))

HSP 1 Score: 187.6 bits (475), Expect = 8.3e-48
Identity = 103/221 (46.61%), Postives = 135/221 (61.09%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDL---CKYPRSPALGLGL 60
           MER+  +M   V+ +LG++   T F AEATR K +QVT    D    C YPRSPA  LG 
Sbjct: 1   MERRKIVMCG-VLFLLGLLSAVTAFVAEATRIKRSQVTVTVSDSITKCTYPRSPAFNLGF 60

Query: 61  TAALSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAAL 120
           T+AL L+ AQI +  S+GC CC +GP P  S W  ++ICF +SW T+VIAFL+ L+GAAL
Sbjct: 61  TSALFLMMAQIIVSVSSGCFCCRKGPAPSRSNWIISLICFVVSWFTFVIAFLVLLSGAAL 120

Query: 121 NNGRGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPS 180
           N+   E+      Y CY++KPGVFS   ++ + ++ LG+ Y+L L S K   +      +
Sbjct: 121 NDEHTEESMNAGTYFCYIVKPGVFSTGAVLSLVTIALGIVYYLCLTSNKQIVAA-----T 180

Query: 181 VPPQPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
                 IAM QPQ       P+R  DPVFVHEDTYMRRQFT
Sbjct: 181 TTQGTGIAMGQPQI------PERVEDPVFVHEDTYMRRQFT 209

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BLAST of CSPI04G04390.1 vs. TAIR10
Match: AT4G27435.1 (AT4G27435.1 Protein of unknown function (DUF1218))

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 48/164 (29.27%), Postives = 79/164 (48.17%), Query Frame = 1


Query: 8   MVEYVVAILGIVMIATGFAAEATRTKGNQV--TKVAPDLCKYPRSPALGLGLTAALSLLF 67
           +V  +V +  ++      AAE  R+    V  T+V  + C Y    A G G+ A L  + 
Sbjct: 5   IVSAIVFVFNLIAFGLAVAAEQRRSTARVVQDTEVQYNYCVYDSDRATGYGVGAFLFSVA 64

Query: 68  AQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNGRGEQR 127
           +QI I   + C CC +  +P  S+   A+I F +SW+ ++IA +  L G+  N    + R
Sbjct: 65  SQILIMLVSRCFCCGKPLKPGGSR-ALALILFIVSWMFFLIAEICLLAGSVENAYHTKYR 124

Query: 128 NYFRDY--DCYVLKPGVFSF-ATIVGMASLTLGMSYFLILNSAK 167
             F D   DC  L+ GVF+  A+ V   ++     YF   ++A+
Sbjct: 125 TMFMDNPPDCQTLRKGVFAAGASFVFFNAIVSQFYYFFYFSAAE 167

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BLAST of CSPI04G04390.1 vs. TAIR10
Match: AT1G61065.1 (AT1G61065.1 Protein of unknown function (DUF1218))

HSP 1 Score: 63.2 bits (152), Expect = 2.4e-10
Identity = 48/168 (28.57%), Postives = 85/168 (50.60%), Query Frame = 1


Query: 5   SALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDL--CKYPRSPALGLGLTAALS 64
           +++++  +V +  ++      AAE  RT   Q+++ + DL  C Y +  A GLG+ + L 
Sbjct: 2   ASVLLLLLVFVFDLIAFGLAVAAEQRRTTW-QISRESRDLSYCVYDKDIATGLGVGSFLV 61

Query: 65  LLFAQITIKASTGCVCCIRGPRPPASK-WRTAVICFTISWVTYVIAFLLFLTGAALNNGR 124
           LL +Q+ I  ++ C+CC R   P  S+ W  A+  F  +WV + IA +  L G+  N   
Sbjct: 62  LLASQLLIMVASRCLCCGRALTPSGSRSW--AIFLFITTWVFFFIAQVCLLAGSVRNAYH 121

Query: 125 GEQRNYFRDY--DCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKN 168
            + R YF +    C  L+ GVF       + +  +   Y++ L+ AK+
Sbjct: 122 TKYRVYFGNTSPSCRSLRKGVFGAGAAFIVLTGIVSELYYVTLSRAKD 166

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BLAST of CSPI04G04390.1 vs. NCBI nr
Match: gi|449458299|ref|XP_004146885.1| (PREDICTED: uncharacterized protein LOC101221478 [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-118
Identity = 216/218 (99.08%), Postives = 217/218 (99.54%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERKSALMV+YVVAILGIVMIATGFAAEATRTK NQVTKVAPDLCKYPRSPALGLGLTAA
Sbjct: 1   MERKSALMVKYVVAILGIVMIATGFAAEATRTKRNQVTKVAPDLCKYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG
Sbjct: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
           RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP
Sbjct: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
           QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT
Sbjct: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 218

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BLAST of CSPI04G04390.1 vs. NCBI nr
Match: gi|659107725|ref|XP_008453825.1| (PREDICTED: uncharacterized protein LOC103494435 [Cucumis melo])

HSP 1 Score: 395.6 bits (1015), Expect = 5.7e-107
Identity = 196/220 (89.09%), Postives = 209/220 (95.00%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK+AL+V++VVA LGIVMIATGFAAEATRTKG QVT+VAPD+CKYPRSPA+GLG TAA
Sbjct: 1   MERKAALVVKFVVAFLGIVMIATGFAAEATRTKGKQVTRVAPDVCKYPRSPAMGLGFTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           LSLLFAQITIKASTGCVCCIRGPRPP  KWRTAVICFT+SW+TYVIAFLLFLTGAALNNG
Sbjct: 61  LSLLFAQITIKASTGCVCCIRGPRPP--KWRTAVICFTVSWITYVIAFLLFLTGAALNNG 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
           R +QRNY  +Y+CYVLKPGVFSFATIVG+ASLTLGMSYFLILNSAKNDPSTVWGHPSVPP
Sbjct: 121 RDQQRNYLGNYECYVLKPGVFSFATIVGVASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180

Query: 181 QPNIAMAQPQFPN--PPPPPQRTADPVFVHEDTYMRRQFT 219
           QPNIAMAQPQFPN  PPPPPQRT DPVFVHEDTYMRRQFT
Sbjct: 181 QPNIAMAQPQFPNPPPPPPPQRTTDPVFVHEDTYMRRQFT 218

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BLAST of CSPI04G04390.1 vs. NCBI nr
Match: gi|641833512|gb|KDO52524.1| (hypothetical protein CISIN_1g028344mg [Citrus sinensis])

HSP 1 Score: 259.6 bits (662), Expect = 4.9e-66
Identity = 133/218 (61.01%), Postives = 162/218 (74.31%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK  L +   V +LG++  ATGF AEATR KG+QV   +P  C YPRSPALGLGLTAA
Sbjct: 1   MERK-VLALCSTVGLLGLLSAATGFGAEATRIKGSQVQITSPTQCSYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           LSLL AQ+TI  +TGC+CC RGP P  S W  A++CF +SW T+VIAFLL LTGAALN+ 
Sbjct: 61  LSLLIAQVTINVATGCICCRRGPHPSNSNWTIALVCFVVSWFTFVIAFLLLLTGAALNDQ 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
            GE+  YF +Y CYV+KPGVF+   ++ +AS+TLG+ Y+L L+SAKN  S +WG+ SVP 
Sbjct: 121 HGEESMYFGNYYCYVVKPGVFAGGAVLSLASVTLGILYYLTLHSAKN--SGLWGNSSVPQ 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
           +  IAM QPQF     PPQRT +PVFVHEDTYMRRQFT
Sbjct: 181 EGGIAMGQPQF-----PPQRTQEPVFVHEDTYMRRQFT 210

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BLAST of CSPI04G04390.1 vs. NCBI nr
Match: gi|568877605|ref|XP_006491818.1| (PREDICTED: uncharacterized protein LOC102608001 [Citrus sinensis])

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-65
Identity = 132/218 (60.55%), Postives = 162/218 (74.31%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK  L +   V +LG++  ATGF AEATR KG+QV   +P  C YPRSPALGLGLTAA
Sbjct: 1   MERK-VLALCSTVGLLGLLSAATGFGAEATRIKGSQVQITSPTQCSYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           LSLL AQ+TI  +TGC+CC RGP P  S W  A++CF +SW T+VIAFLL LTGAALN+ 
Sbjct: 61  LSLLIAQVTINVATGCICCRRGPHPSNSNWTIALVCFVVSWFTFVIAFLLLLTGAALNDQ 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
            GE+  YF +Y CYV+KPGVF+   ++ +AS+TLG+ Y+L L+SAKN  S +WG+ SVP 
Sbjct: 121 HGEESMYFGNYYCYVVKPGVFAGGAVLSLASVTLGILYYLTLHSAKN--SGLWGNSSVPQ 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQFT 219
           +  IAM QPQF     PPQRT +PVFVHEDT+MRRQFT
Sbjct: 181 EGGIAMGQPQF-----PPQRTQEPVFVHEDTFMRRQFT 210

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BLAST of CSPI04G04390.1 vs. NCBI nr
Match: gi|661891067|emb|CDP04949.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-59
Identity = 123/216 (56.94%), Postives = 154/216 (71.30%), Query Frame = 1


Query: 1   MERKSALMVEYVVAILGIVMIATGFAAEATRTKGNQVTKVAPDLCKYPRSPALGLGLTAA 60
           MERK  ++V  VV  LG++  ATGFAAEATR KG+QV   +PD+C YPRSPALGLGLTAA
Sbjct: 1   MERK-VIVVCCVVGFLGLLSAATGFAAEATRIKGDQVKFPSPDVCVYPRSPALGLGLTAA 60

Query: 61  LSLLFAQITIKASTGCVCCIRGPRPPASKWRTAVICFTISWVTYVIAFLLFLTGAALNNG 120
           ++L+ AQ+ +  +TGC+CC RGP    S W+ A++CF +SW T+V+AFLL LTGAALN+ 
Sbjct: 61  VTLMIAQVIVNVATGCICCRRGPHQANSNWKLALVCFIVSWFTFVVAFLLLLTGAALNDQ 120

Query: 121 RGEQRNYFRDYDCYVLKPGVFSFATIVGMASLTLGMSYFLILNSAKNDPSTVWGHPSVPP 180
            GE+  YF  Y CYV+KPGVF+ A I+ +AS+TLG+ Y+++L S KN     WG  S P 
Sbjct: 121 HGEENMYFNYYYCYVVKPGVFAGAAILSLASVTLGLIYYVMLTSTKN-VIDAWGGSSAPV 180

Query: 181 QPNIAMAQPQFPNPPPPPQRTADPVFVHEDTYMRRQ 217
              IAM QPQF     P Q T  PVFVHEDTYMRRQ
Sbjct: 181 PGGIAMGQPQF-----PAQNTEAPVFVHEDTYMRRQ 209

The following BLAST results are available for this feature:

BLAST of CSPI04G04390.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI04G04390.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU80_CUCSA	7.7e-119	99.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025740 PE=4 SV=1	[more]
A0A067EBD4_CITSI	3.4e-66	61.01	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g028344mg PE=4 SV=1	[more]
A0A068U9S1_COFCA	1.1e-59	56.94	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00019822001 PE=4 SV=1	[more]
D7T7U5_VITVI	7.6e-58	56.70	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0039g02520 PE=4 SV=...	[more]
M5X117_PRUPE	9.9e-58	56.16	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011406mg PE=4 SV=1	[more]

BLAST of CSPI04G04390.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G17210.1	8.3e-48	46.61	Protein of unknown function (DUF1218)	[more]
AT4G27435.1	1.8e-10	29.27	Protein of unknown function (DUF1218)	[more]
AT1G61065.1	2.4e-10	28.57	Protein of unknown function (DUF1218)	[more]

BLAST of CSPI04G04390.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449458299\|ref\|XP_004146885.1\|	1.1e-118	99.08	PREDICTED: uncharacterized protein LOC101221478 [Cucumis sativus]	[more]
gi\|659107725\|ref\|XP_008453825.1\|	5.7e-107	89.09	PREDICTED: uncharacterized protein LOC103494435 [Cucumis melo]	[more]
gi\|641833512\|gb\|KDO52524.1\|	4.9e-66	61.01	hypothetical protein CISIN_1g028344mg [Citrus sinensis]	[more]
gi\|568877605\|ref\|XP_006491818.1\|	1.4e-65	60.55	PREDICTED: uncharacterized protein LOC102608001 [Citrus sinensis]	[more]
gi\|661891067\|emb\|CDP04949.1\|	1.5e-59	56.94	unnamed protein product [Coffea canephora]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR009606	DUF1218

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G04390	CSPI04G04390	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G04390.1	CSPI04G04390.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G04390.1.utr5p1	CSPI04G04390.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G04390.1.cds1	CSPI04G04390.1.cds1	CDS
CSPI04G04390.1.cds2	CSPI04G04390.1.cds2	CDS
CSPI04G04390.1.cds3	CSPI04G04390.1.cds3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G04390.1.utr3p1	CSPI04G04390.1.utr3p1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009606	Protein of unknown function DUF1218	PFAM	PF06749	DUF1218	coord: 59..151 score: 1.7
None	No IPR available	PANTHER	PTHR31769	FAMILY NOT NAMED	coord: 1..217 score: 5.1
None	No IPR available	PANTHER	PTHR31769:SF8	SUBFAMILY NOT NAMED	coord: 1..217 score: 5.1