CSPI04G01930.2 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G01930.2
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Calcineurin subunit B
Location	Chr4 : 1110371 .. 1113593 (-)
Sequence length	528

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCAACAACATCAGTTTTTTAAATCTAACCCTAAAAATTTGAAAGGTTAAAATTCAGCTTAATTCTAATTTTATTTAGGACCAGATTTACAATTTAAACAGATTTTTTTTTTTTTTTCCTCTAGAATTCAGATCATTTCATGCAAGCAAAAGCAGCTAAGGGCGCAGTTCTCAGCTCCAGATCGGGCAGCTTTCTCTTCTTTTGATCCGATTCTGTTTGGGTTCTTGAAATTTTTTATGGTCAATCAACAAACAACTCCGCAGAATTCAATTAATTCGTCCGCTTAGTTTGATAAGTTTTTTGATCTTCAGCGACTCCTACAGTTTGTTTTGATTTCTCCCTTCTCTACTATAATAGTAAATTCTCTGCAATCTACCTACATTTTGCCTCTTCTTCGAGGTGAGAAGGTAGATTTTGATTTGCGCGTGTATTGAATCTCAGCAACAAACAATGGGAAACTCCTCTTCGATGCTCACTCAATATGATATTGAAGAAGTTCAATGCCACTGCGATAATCTATGTGAGATTTTTTGTTTTTTTCGATTTATGTTCCTTATCCTTTCTTTCCATACTCTTCACTTCTAATTTAACTCGGTTCTCTTTCAATTTGGTTGTATAATTCAGTTACGCAACAAGAAATAGTGTCACTGTATCAAAGATTTTGTCAACTCGATCGAAACTCAAAGGGATACATCTTGGCCGATGAGTTTTTATCAGTCCCAGAATTCGCTATGAATCCTCTTTCTCAGGTTTTCTTTTGAAACATTTCTATGATTGTTTTCTTGAAATTGCGTGTACTTTAATCGGTGAATGATAGTTTGTCCTTTCCTTTTTGTGTTCCCCAGAGGCTGCTAAAGATGGTTGATGGTTTGAACTTTAAGGATTTCGTGGCGTTTTTGTCTGCATTTAGTGCTAAAGCTAGCATCCAACGTAAAATTGAATGTACGTATAAAATGCGTACTTGCTATTGTTTAGATGCTGTCTGATGATACTCGACATTTGATTTCTTTGACAGTATTCCAAATATTGCCTTTGAAGTTTGGAGCAAATTTTTGGTTTATATTTTTCACTTTTCATACTTAATTGTACAGAAATGCTATGGAATAATGCACTTTTCAGTTTCCATTTAAGACGTCTTTTCCCCAGAAATATTACATGATCTGCTCATTGAAATATAGAAACAAATGGCGGTATACTTATGTTCTTGAATTTGATGGTATTGGGAATTTATGAGGTTGATATGGACAAAAGAACCTCCACCTATCTTTACATTGATAAGATATTGCCCACTTTGGACATAAGCATTTATAGCTTTGCTTTTGATTTCACCCAGAAGGTTATATTGGTGGAGATGATTGTCCATACTTATGTACACTAGATCTCTCCCTTTCCTAAACAACGAAGGACTTTGTTTACGCTCCTAGCAATTACTATCCAATATGTTTGTAGTTTTCTTCTAGAATTATAGATGGTGAGAAAAGGTTATATCAAATGGGCTTCTATAATGAGTCCTCAAAATCAACTTGTTGTAATTGGCTCCATAGGACCTTCATAGATAGTGTAATTTGGTATTTTTAGTTGGTTGATGTACAAAAATCGTAAGAGTTATCTGCATGATTTTGGGTAGCCGTTCTAATAATTTTTTTAAATAGTGTGAGTATTGGGTTAAAATGTATATGAGTATATCTTAATGAACTTTCTTAGTCAAATAACATTCTTCATTTATTGTGAGAGGGGTTGAATTCAGTGGTTAAATTTCTTTCTACCTATCAGAAACATGGCACTGTGTATCTCCTCTTTTCTTTTCTTGTCAATCGTTTTCTTTAGCCACTAACATCATACTCTTTTAACTAAAAATTTGATTATCTAAAATTTTAATAAATGATATACTGATATAATGAGCTTTGTTGTCTTGAAAAAGCTTGACTCATCAGTTATTATTTGTTTTTGGTTCCATCAGTTATGTTCAAAGTTTATGATGCCGACTTGAATGGGAAGGTGTCCTTCAATGACATGATAGAAGTGCTACAGGATTTGTCTGGTCCCTTCATGTCTGATGACCAAAGAAAGGTACTCTAGACGATACATTAAATCTTATGAGGCATTGAGAGAATTGTATATATTTTCCATGGCACATGGCATGTTATAAAGTTACGTTCATTTCTTTCTTTTGCAGCAAGTCTTGAGCCAACTTCTGGAGGAAGCAGGTTACACAAAGGAAGTCCATTTAACCCAGGATGATTTCGTTAAGGTTTGTTTTTACCATGTTAAATCTCATTCCGTTCATTTGTACCAACAAGACCTAGTGGGCCAGTGACTTCCTAATCTGACACGCATACAAGCACAAAAATAATTAATAAAATTATTTGAAACTTGGATCAATTAAATGGAGAAAGTACATTAATAGTCTAAATTACCCAAATATTACCACAAAGATAAAGCAGCTGAAATCTCAAGGACAATTTGTGATCAATAAATAGTCTAGTCATTAACGTTAACAATCATATTTGAAATCTTAAGAAAATTTATACCTCTATCAATAACAGTACCTCGTTGTTGAACTTTAAACTTCAATTTTGAAATTGTTTTTGATTTAAACTTTACTGATTCTTCAGCATCTGAATGAAAAGGGTTCCACCAATCATGGTTTATTTGCTTATTCTATACACTTCAGAACTACTTCATTATTCATCACTTGGTGTCTTGCACCATGTCAACTTTTCCATGATTTTTCCTAGAAGTCATCTAGTTCAATTTTGTGATCCCGTGCTAACTATTATTGTACTGACTTTTGTCATCTGAGTTTGCTGTAAGTCATTCATAATTTTCTCAATTCATATATTCCCCAAGCTGTTGTAATGTGTATTTTTGTTCTTTCGCAGATTCTTGCCAATTCTGACCTGAAAATGGAGGTTGAGGTGCCTGTTGATTAAGACGACTACTCAAACTATTCCTTTGTCCCCTCCCTTTTCCTCTTTCGCGACAAGGAGAAACAAATGATAAATTACTAGCTAAAGCGTCATCGTCATGTATCATGTCTTCGTCATCCTCATGTATCATGTCCTTCTCAAGCAATTTCAGCGTCGATGAGCAAGATCAATTCTTCAAACATGTTCATTTATGCTCAATTCAAGCCTATGGCTCACCCTTTTGTAGACAGATATTGATTTCAAAGATTACGAACCATCTTAATGAAAATCTCTCTGAAACGAATTATTTCTG

mRNA sequence

ATGGGAAACTCCTCTTCGATGCTCACTCAATATGATATTGAAGAAGTTCAATGCCACTGCGATAATCTATTTACGCAACAAGAAATAGTGTCACTGTATCAAAGATTTTGTCAACTCGATCGAAACTCAAAGGGATACATCTTGGCCGATGAGTTTTTATCAGTCCCAGAATTCGCTATGAATCCTCTTTCTCAGAGGCTGCTAAAGATGGTTGATGGTTTGAACTTTAAGGATTTCGTGGCGTTTTTGTCTGCATTTAGTGCTAAAGCTAGCATCCAACGTAAAATTGAATTTATGTTCAAAGTTTATGATGCCGACTTGAATGGGAAGGTGTCCTTCAATGACATGATAGAAGTGCTACAGGATTTGTCTGGTCCCTTCATGTCTGATGACCAAAGAAAGCAAGTCTTGAGCCAACTTCTGGAGGAAGCAGGTTACACAAAGGAAGTCCATTTAACCCAGGATGATTTCGTTAAGATTCTTGCCAATTCTGACCTGAAAATGGAGGTTGAGGTGCCTGTTGATTAA

Coding sequence (CDS)

ATGGGAAACTCCTCTTCGATGCTCACTCAATATGATATTGAAGAAGTTCAATGCCACTGCGATAATCTATTTACGCAACAAGAAATAGTGTCACTGTATCAAAGATTTTGTCAACTCGATCGAAACTCAAAGGGATACATCTTGGCCGATGAGTTTTTATCAGTCCCAGAATTCGCTATGAATCCTCTTTCTCAGAGGCTGCTAAAGATGGTTGATGGTTTGAACTTTAAGGATTTCGTGGCGTTTTTGTCTGCATTTAGTGCTAAAGCTAGCATCCAACGTAAAATTGAATTTATGTTCAAAGTTTATGATGCCGACTTGAATGGGAAGGTGTCCTTCAATGACATGATAGAAGTGCTACAGGATTTGTCTGGTCCCTTCATGTCTGATGACCAAAGAAAGCAAGTCTTGAGCCAACTTCTGGAGGAAGCAGGTTACACAAAGGAAGTCCATTTAACCCAGGATGATTTCGTTAAGATTCTTGCCAATTCTGACCTGAAAATGGAGGTTGAGGTGCCTGTTGATTAA

Homology

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BLAST of CSPI04G01930.2 vs. Swiss-Prot
Match: CANB1_NAEGR (Calcineurin subunit B OS=Naegleria gruberi GN=CNB1 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.5e-28
Identity = 68/177 (38.42%), Postives = 114/177 (64.41%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MG ++S L   ++EE+Q   +  FTQ+EI  LY+RF +LD++  G I  DEFL +PE A+
Sbjct: 1   MGTNTSSLRPEEVEEMQKGTN--FTQKEIKKLYKRFKKLDKDGNGTISKDEFLMIPELAV 60

Query: 61  NPLSQRLLKMVD-----GLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFND 120
           NPL +R++ + D      +NFK+F+A LS F+A+   QRK+EF FKVYD D +G +S  +
Sbjct: 61  NPLVKRVISIFDENGDGSVNFKEFIAALSVFNAQGDKQRKLEFAFKVYDIDGDGYISNGE 120

Query: 121 MIEVLQDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEV 173
           +  VL+ + G  +SD Q +Q++ + + EA    +  ++ ++F K L++ DL+ ++ +
Sbjct: 121 LFTVLKMMVGNNLSDVQLQQIVDKTILEADEDGDGKISFEEFAKTLSHQDLENKMTI 175

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BLAST of CSPI04G01930.2 vs. Swiss-Prot
Match: CANB1_DICDI (Calcineurin subunit B type 1 OS=Dictyostelium discoideum GN=cnbA PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-23
Identity = 53/170 (31.18%), Postives = 107/170 (62.94%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN  S+L +  +E+++ +    F++ E+  LY+RF  LD++  G +  DEFLS+P+ A+
Sbjct: 1   MGNQHSLLNKEQLEQMKDNSS--FSEAELKKLYRRFQMLDKDGSGTLTTDEFLSIPDLAL 60

Query: 61  NPLSQRLLKMVDG-----LNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFND 120
           NPL +R++++ D      + F +FV  L+  S K + + K++F+F++YD D +G +S  +
Sbjct: 61  NPLLERVIQIFDQNKDNEIEFFEFVGTLATLSHKGTKEDKLKFLFQIYDIDCDGFISNGE 120

Query: 121 MIEVLQDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSD 166
           + +VL+ + G  ++D Q +Q++ + + E  Y K+  ++ D+F+ ++ N +
Sbjct: 121 LFQVLKMMVGTNLNDVQLQQIVDKTIIEGDYDKDGKISFDEFIHMIGNQE 168

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BLAST of CSPI04G01930.2 vs. Swiss-Prot
Match: CANB_NEUCR (Calcineurin subunit B OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=cnb-1 PE=2 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 2.0e-22
Identity = 56/175 (32.00%), Postives = 110/175 (62.86%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN++S +    ++       + F ++E+  L +RF +LD+++ G I  +EFLS+P+ + 
Sbjct: 1   MGNTTSSVLDNIVQ------GSNFDREEVDRLRKRFMKLDKDNSGTIEREEFLSLPQIST 60

Query: 61  NPLSQRLLKMVD-----GLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFND 120
           NPL+ R++ + D      ++F++FV+ LSAFS+K + ++K+ F FKVYD D +G +S  +
Sbjct: 61  NPLATRMIAIFDEDGGGDVDFQEFVSGLSAFSSKGNKEQKLRFAFKVYDIDRDGYISNGE 120

Query: 121 MIEVLQDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEV 171
           +  VL+ + G  + D Q +Q++ + + EA   K+  ++ ++F K++ N+D+ M +
Sbjct: 121 LFIVLKMMVGSNLKDQQLQQIVDKTIMEADLDKDGKISFEEFTKMVENTDVSMSM 169

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BLAST of CSPI04G01930.2 vs. Swiss-Prot
Match: CANB_CRYNB (Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) GN=CNB1 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.2e-20
Identity = 51/148 (34.46%), Postives = 92/148 (62.16%), Query Frame = 1


Query: 24  FTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAMNPLSQRLLKMVDG-----LNFKD 83
           F+  E++ L +RF +LD++  G I  DEFL +P+ A NPL+ R++ + D      ++F++
Sbjct: 18  FSGPELMRLKKRFMKLDKDGSGSIDKDEFLQIPQIANNPLAHRMIAIFDEDGSGTVDFQE 77

Query: 84  FVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVLQDLSGPFMSDDQRKQVLS 143
           FV  LSAFS+K     K+ F FKVYD D +G +S  ++  VL+ + G  + D Q +Q++ 
Sbjct: 78  FVGGLSAFSSKGGRDEKLRFAFKVYDMDRDGYISNGELYLVLKQMVGNNLKDQQLQQIVD 137

Query: 144 QLLEEAGYTKEVHLTQDDFVKILANSDL 167
           + + EA    +  L+ ++F +++A++D+
Sbjct: 138 KTIMEADKDGDGKLSFEEFTQMVASTDI 165

[Close]

BLAST of CSPI04G01930.2 vs. Swiss-Prot
Match: CANB_CRYNJ (Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) GN=CNB1 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.2e-20
Identity = 51/148 (34.46%), Postives = 92/148 (62.16%), Query Frame = 1


Query: 24  FTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAMNPLSQRLLKMVDG-----LNFKD 83
           F+  E++ L +RF +LD++  G I  DEFL +P+ A NPL+ R++ + D      ++F++
Sbjct: 18  FSGPELMRLKKRFMKLDKDGSGSIDKDEFLQIPQIANNPLAHRMIAIFDEDGSGTVDFQE 77

Query: 84  FVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVLQDLSGPFMSDDQRKQVLS 143
           FV  LSAFS+K     K+ F FKVYD D +G +S  ++  VL+ + G  + D Q +Q++ 
Sbjct: 78  FVGGLSAFSSKGGRDEKLRFAFKVYDMDRDGYISNGELYLVLKQMVGNNLKDQQLQQIVD 137

Query: 144 QLLEEAGYTKEVHLTQDDFVKILANSDL 167
           + + EA    +  L+ ++F +++A++D+
Sbjct: 138 KTIMEADKDGDGKLSFEEFTQMVASTDI 165

[Close]

BLAST of CSPI04G01930.2 vs. TrEMBL
Match: A0A0A0KTI1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006370 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 1.3e-92
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM
Sbjct: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD
Sbjct: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. TrEMBL
Match: Q7FZ95_EUCGR (Calcineurin-like protein OS=Eucalyptus grandis GN=EgCBL1 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.0e-76
Identity = 145/175 (82.86%), Postives = 164/175 (93.71%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+NLF+QQEIVSLY+RFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 1   MGNASSMLTQYDIEEVQDHCNNLFSQQEIVSLYERFCQLDRNAKGFISADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS Q+KIE +FKVYD+D NGKVSFND++EVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASKQQKIELIFKVYDSDCNGKVSFNDILEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPFMSD+QR+QVL Q+L+EAGYT+E +L  DDFVK+  NSDLKMEVEVPVD
Sbjct: 121 RDLSGPFMSDEQREQVLVQVLKEAGYTRESYLLLDDFVKVFGNSDLKMEVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. TrEMBL
Match: Q93VF2_EUCCA (Calcineurin-like protein OS=Eucalyptus camaldulensis GN=EcCBL1 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.0e-76
Identity = 145/175 (82.86%), Postives = 164/175 (93.71%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+NLF+QQEIVSLY+RFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 1   MGNASSMLTQYDIEEVQDHCNNLFSQQEIVSLYERFCQLDRNAKGFISADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS Q+KIE +FKVYD+D NGKVSFND++EVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASKQQKIELIFKVYDSDCNGKVSFNDILEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPFMSD+QR+QVL Q+L+EAGYT+E +L  DDFVK+  NSDLKMEVEVPVD
Sbjct: 121 RDLSGPFMSDEQREQVLVQVLKEAGYTRESYLLLDDFVKVFGNSDLKMEVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. TrEMBL
Match: A0A059BYK8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03918 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.0e-76
Identity = 145/175 (82.86%), Postives = 164/175 (93.71%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+NLF+QQEIVSLY+RFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 63  MGNASSMLTQYDIEEVQDHCNNLFSQQEIVSLYERFCQLDRNAKGFISADEFLSVPEFAM 122

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS Q+KIE +FKVYD+D NGKVSFND++EVL
Sbjct: 123 NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASKQQKIELIFKVYDSDCNGKVSFNDILEVL 182

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPFMSD+QR+QVL Q+L+EAGYT+E +L  DDFVK+  NSDLKMEVEVPVD
Sbjct: 183 RDLSGPFMSDEQREQVLVQVLKEAGYTRESYLLLDDFVKVFGNSDLKMEVEVPVD 237

[Close]

BLAST of CSPI04G01930.2 vs. TrEMBL
Match: A0A0A0KBS0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G432270 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 3.8e-76
Identity = 144/175 (82.29%), Postives = 162/175 (92.57%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+ LF+QQEIVSLYQRFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 1   MGNASSMLTQYDIEEVQEHCNKLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS+  KIEF+FKVYDAD NGKVSFN++IEVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASVHHKIEFIFKVYDADCNGKVSFNEIIEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPF+SD+QR+QVL QLL+EAGYT+   LT DDFVK+L NS +KM+VEVPVD
Sbjct: 121 RDLSGPFISDEQREQVLCQLLQEAGYTRNSQLTLDDFVKVLGNSGIKMDVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. TAIR10
Match: AT3G18430.1 (AT3G18430.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 272.7 bits (696), Expect = 1.6e-73
Identity = 132/175 (75.43%), Postives = 159/175 (90.86%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC +LF QQEI+SLYQRFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 1   MGNTSSMLTQYDIEEVQSHCHDLFEQQEILSLYQRFCQLDRNAKGFISADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS+++K++ +FKVYD+D NGKVSF D++EVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASLRQKVQLIFKVYDSDCNGKVSFKDIMEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSG FMSD+QR+QVLSQ+L+E+GYT +  LT +DF+KI  +S  +M+VE+PVD
Sbjct: 121 RDLSGSFMSDEQREQVLSQVLKESGYTSDSFLTLEDFIKIFGSSRPEMDVEIPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. TAIR10
Match: AT3G51920.1 (AT3G51920.1 calmodulin 9)

HSP 1 Score: 55.5 bits (132), Expect = 4.0e-08
Identity = 31/132 (23.48%), Postives = 69/132 (52.27%), Query Frame = 1


Query: 24  FTQQEIVSLYQRFCQLDRNSKGYILADEFLSV-PEFAMNPLSQRLLKMVD--------GL 83
           FT ++I   Y+ FC +D++S G+I  ++   V      NP +++L +M+         G+
Sbjct: 5   FTDEQIQEFYEAFCLIDKDSDGFITKEKLTKVMKSMGKNPKAEQLQQMMSDVDIFGNGGI 64

Query: 84  NFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVLQDLSGPFMSDDQRK 143
            F DF+  ++  +++ S   ++  +F+V+D D +G +S  ++ E ++D+     +++   
Sbjct: 65  TFDDFLYIMAQNTSQESASDELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEH 124

Query: 144 QVLSQLLEEAGY 147
            V    L+  G+
Sbjct: 125 MVREADLDGDGF 136

[Close]

BLAST of CSPI04G01930.2 vs. TAIR10
Match: AT5G47100.1 (AT5G47100.1 calcineurin B-like protein 9)

HSP 1 Score: 49.7 bits (117), Expect = 2.2e-06
Identity = 39/176 (22.16%), Postives = 83/176 (47.16%), Query Frame = 1


Query: 16  VQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM---------NPLSQR 75
           V+   +  F+  E+ +LY+ F  +  +    ++ D  ++  EF +         N  + R
Sbjct: 19  VKLASETAFSVSEVEALYELFKSISSS----VVDDGLINKEEFQLALFKNRKKENLFANR 78

Query: 76  LLKMVDG-----LNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVLQ 135
           +  + D      ++F DFV  L+ F   AS++ K +F F++YD D  G +   ++ ++L 
Sbjct: 79  IFDLFDVKRKGVIDFGDFVRSLNVFHPNASLEEKTDFTFRLYDMDCTGFIERQEVKQMLI 138

Query: 136 DL---SGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVK-ILANSDLKMEVEVP 174
            L   S   ++DD  + +L Q  E+A   ++  + + ++   ++ N  L   + +P
Sbjct: 139 ALLCESEMKLADDTIEMILDQTFEDADVDRDGKIDKTEWSNFVIKNPSLLKIMTLP 190

[Close]

BLAST of CSPI04G01930.2 vs. TAIR10
Match: AT4G16350.1 (AT4G16350.1 calcineurin B-like protein 6)

HSP 1 Score: 48.9 bits (115), Expect = 3.7e-06
Identity = 42/134 (31.34%), Postives = 68/134 (50.75%), Query Frame = 1


Query: 23  LFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAMNP----LSQRLLKMVDG----- 82
           +FT  EI +LY+ F  + +N  G I  ++F  V  F MN      + R+  + D      
Sbjct: 39  VFTVNEIEALYELFKSISKN--GLIDKEQFQLVL-FKMNTTRSLFADRVFDLFDTKNTGI 98

Query: 83  LNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIE-VLQDL--SGPFMSD 142
           L+F+ F   LS F   A  + KIEF FK+YD +  G +   ++ + V++ L  SG  +SD
Sbjct: 99  LDFEAFARSLSVFHPNAKFEDKIEFSFKLYDLNQQGYIKRQEVKQMVVRTLAESGMNLSD 158

Query: 143 DQRKQVLSQLLEEA 145
              + ++ +  EEA
Sbjct: 159 HVIESIIDKTFEEA 169

[Close]

BLAST of CSPI04G01930.2 vs. NCBI nr
Match: gi|449468944|ref|XP_004152181.1| (PREDICTED: calcineurin subunit B-like [Cucumis sativus])

HSP 1 Score: 347.1 bits (889), Expect = 1.9e-92
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM
Sbjct: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD
Sbjct: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. NCBI nr
Match: gi|659108419|ref|XP_008454189.1| (PREDICTED: calcineurin subunit B-like [Cucumis melo])

HSP 1 Score: 343.6 bits (880), Expect = 2.1e-91
Identity = 173/175 (98.86%), Postives = 174/175 (99.43%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGNSSSMLTQYDIEEVQCHCD+LFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM
Sbjct: 1   MGNSSSMLTQYDIEEVQCHCDDLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKIL NSDLKMEVEVPVD
Sbjct: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILGNSDLKMEVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. NCBI nr
Match: gi|629105281|gb|KCW70750.1| (hypothetical protein EUGRSUZ_F03918 [Eucalyptus grandis])

HSP 1 Score: 294.3 bits (752), Expect = 1.4e-76
Identity = 145/175 (82.86%), Postives = 164/175 (93.71%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+NLF+QQEIVSLY+RFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 63  MGNASSMLTQYDIEEVQDHCNNLFSQQEIVSLYERFCQLDRNAKGFISADEFLSVPEFAM 122

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS Q+KIE +FKVYD+D NGKVSFND++EVL
Sbjct: 123 NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASKQQKIELIFKVYDSDCNGKVSFNDILEVL 182

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPFMSD+QR+QVL Q+L+EAGYT+E +L  DDFVK+  NSDLKMEVEVPVD
Sbjct: 183 RDLSGPFMSDEQREQVLVQVLKEAGYTRESYLLLDDFVKVFGNSDLKMEVEVPVD 237

[Close]

BLAST of CSPI04G01930.2 vs. NCBI nr
Match: gi|706715452|ref|NP_001289640.1| (calcineurin subunit B [Eucalyptus grandis])

HSP 1 Score: 294.3 bits (752), Expect = 1.4e-76
Identity = 145/175 (82.86%), Postives = 164/175 (93.71%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+NLF+QQEIVSLY+RFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 1   MGNASSMLTQYDIEEVQDHCNNLFSQQEIVSLYERFCQLDRNAKGFISADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS Q+KIE +FKVYD+D NGKVSFND++EVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASKQQKIELIFKVYDSDCNGKVSFNDILEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPFMSD+QR+QVL Q+L+EAGYT+E +L  DDFVK+  NSDLKMEVEVPVD
Sbjct: 121 RDLSGPFMSDEQREQVLVQVLKEAGYTRESYLLLDDFVKVFGNSDLKMEVEVPVD 175

[Close]

BLAST of CSPI04G01930.2 vs. NCBI nr
Match: gi|449436166|ref|XP_004135865.1| (PREDICTED: calcineurin subunit B [Cucumis sativus])

HSP 1 Score: 292.4 bits (747), Expect = 5.5e-76
Identity = 144/175 (82.29%), Postives = 162/175 (92.57%), Query Frame = 1


Query: 1   MGNSSSMLTQYDIEEVQCHCDNLFTQQEIVSLYQRFCQLDRNSKGYILADEFLSVPEFAM 60
           MGN+SSMLTQYDIEEVQ HC+ LF+QQEIVSLYQRFCQLDRN+KG+I ADEFLSVPEFAM
Sbjct: 1   MGNASSMLTQYDIEEVQEHCNKLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAM 60

Query: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASIQRKIEFMFKVYDADLNGKVSFNDMIEVL 120
           NPLSQRLLKMVDGLNFKDFVAFLSAFSAKAS+  KIEF+FKVYDAD NGKVSFN++IEVL
Sbjct: 61  NPLSQRLLKMVDGLNFKDFVAFLSAFSAKASVHHKIEFIFKVYDADCNGKVSFNEIIEVL 120

Query: 121 QDLSGPFMSDDQRKQVLSQLLEEAGYTKEVHLTQDDFVKILANSDLKMEVEVPVD 176
           +DLSGPF+SD+QR+QVL QLL+EAGYT+   LT DDFVK+L NS +KM+VEVPVD
Sbjct: 121 RDLSGPFISDEQREQVLCQLLQEAGYTRNSQLTLDDFVKVLGNSGIKMDVEVPVD 175

The following BLAST results are available for this feature:

BLAST of CSPI04G01930.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CANB1_NAEGR	5.5e-28	38.42	Calcineurin subunit B OS=Naegleria gruberi GN=CNB1 PE=3 SV=1	[more]
CANB1_DICDI	1.4e-23	31.18	Calcineurin subunit B type 1 OS=Dictyostelium discoideum GN=cnbA PE=1 SV=1	[more]
CANB_NEUCR	2.0e-22	32.00	Calcineurin subunit B OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS...	[more]
CANB_CRYNB	3.2e-20	34.46	Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (str...	[more]
CANB_CRYNJ	3.2e-20	34.46	Calcineurin subunit B OS=Cryptococcus neoformans var. neoformans serotype D (str...	[more]

BLAST of CSPI04G01930.2 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTI1_CUCSA	1.3e-92	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006370 PE=4 SV=1	[more]
Q7FZ95_EUCGR	1.0e-76	82.86	Calcineurin-like protein OS=Eucalyptus grandis GN=EgCBL1 PE=2 SV=1	[more]
Q93VF2_EUCCA	1.0e-76	82.86	Calcineurin-like protein OS=Eucalyptus camaldulensis GN=EcCBL1 PE=2 SV=1	[more]
A0A059BYK8_EUCGR	1.0e-76	82.86	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03918 PE=4 SV=1	[more]
A0A0A0KBS0_CUCSA	3.8e-76	82.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G432270 PE=4 SV=1	[more]

BLAST of CSPI04G01930.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G18430.1	1.6e-73	75.43	Calcium-binding EF-hand family protein	[more]
AT3G51920.1	4.0e-08	23.48	calmodulin 9	[more]
AT5G47100.1	2.2e-06	22.16	calcineurin B-like protein 9	[more]
AT4G16350.1	3.7e-06	31.34	calcineurin B-like protein 6	[more]

BLAST of CSPI04G01930.2 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449468944\|ref\|XP_004152181.1\|	1.9e-92	100.00	PREDICTED: calcineurin subunit B-like [Cucumis sativus]	[more]
gi\|659108419\|ref\|XP_008454189.1\|	2.1e-91	98.86	PREDICTED: calcineurin subunit B-like [Cucumis melo]	[more]
gi\|629105281\|gb\|KCW70750.1\|	1.4e-76	82.86	hypothetical protein EUGRSUZ_F03918 [Eucalyptus grandis]	[more]
gi\|706715452\|ref\|NP_001289640.1\|	1.4e-76	82.86	calcineurin subunit B [Eucalyptus grandis]	[more]
gi\|449436166\|ref\|XP_004135865.1\|	5.5e-76	82.29	PREDICTED: calcineurin subunit B [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005773	vacuole
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G01930	CSPI04G01930	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G01930.2	CSPI04G01930.2-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G01930.2.utr3p1	CSPI04G01930.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G01930.2.cds8	CSPI04G01930.2.cds8	CDS
CSPI04G01930.2.cds7	CSPI04G01930.2.cds7	CDS
CSPI04G01930.2.cds6	CSPI04G01930.2.cds6	CDS
CSPI04G01930.2.cds5	CSPI04G01930.2.cds5	CDS
CSPI04G01930.2.cds4	CSPI04G01930.2.cds4	CDS
CSPI04G01930.2.cds3	CSPI04G01930.2.cds3	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G01930.2.utr5p3	CSPI04G01930.2.utr5p3	five_prime_UTR
CSPI04G01930.2.utr5p2	CSPI04G01930.2.utr5p2	five_prime_UTR
CSPI04G01930.2.utr5p1	CSPI04G01930.2.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 27..62 score: 8.516coord: 91..126 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 24..164 score: 5.3
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 22..169 score: 1.42
None	No IPR available	PANTHER	PTHR23056	CALCINEURIN B	coord: 1..165 score: 1.1E
None	No IPR available	PANTHER	PTHR23056:SF52	CALCINEURIN B SUBUNIT (PROTEIN PHOSPHATASE 2B REGULATORY SUBUNIT)-LIKE PROTEIN	coord: 1..165 score: 1.1E