CSPI02G08660.1 (mRNA) Wild cucumber (PI 183967)

Overview
NameCSPI02G08660.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
Descriptionnon-specific phospholipase C4
LocationChr2 : 8206393 .. 8208937 (+)
Sequence length972
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACAAGCAAAAAGAACAAAGCATAATTGGAGCCTGAAGTGAACCAGCAAATAGTAAATTAAACAACTATAAATTGGAACAAAATGGCAACAAAACCCTAAGAACAAAAGAGTTTTTCTCCAAATAACATCACCCGATTAGATTTGAAAGGAATCAAATACATAGACCAACCAGAAAACAAAAACTGAAACGAACTCAACCTTGTGAGAACATAGTGTCTTCAAACGAATAAGCAAATAGACGAATGTAAACTCTAGAAAGGCTTTGGTACCATGCACTTTATACTTTTGGAGAATAATAATCTTATTATATATTTTAATTAGGCTAAAGATACATATGTATAGGCGACTAAGAAACCCTACTAATCTAGGGAATATAAAATTACAATAAAGGACAACTATACATGATGATGATGATGATATAAATACAGATTATAGCAATTATATTTCCTTCCATTGTGTATTAATTTGATCTAACGTAATAACATATTATTTTTTAAAACATAGGAATTTAAGAAAACTGAAATACATAAAGAACTTCCATATCTTCGACTTGGATTTCAAGAGAGATTGTCGAGAAGGAAAGCTACCAAACTACGTAGTGATAGAACAGAGATACTTTGATTTAGCATCACTACCTGGAAATGATGATCATCCTTCCCACGACGTTTCAGAAGGCCAAAAACTCATCAAAGAGGTCTATGAAGCTCTGCGATCCAGTCCACAGTGGAACGAAATCCTCTTCTTGATCACTTACGACGAGCATGGCGGTTTCTTCGATCACGTCCCTCCCCCCTCTGCTGGAGTTCCCAATCCTGACGGCCGACTCGGCCCTCCGCCATACAATTTCAATTTTGATCGCCTTGGAGTTCGAGTTCCGACCATCTTTGTCTCTCCTTGGATTGAACCCGGAACAGGTTTTTTCTTCTTCTTTTAAATCTGTGAATTGTTTGTTTGTTTCAGAATTTCTGGTGTTAGAAAATTTCAATCGAAGCTATTAGTACAGTTACACATAAAAAATGTAAATTTGAGAAGGATGGTGTATGTAAAATACAGTGGTGCACAGACCGAGGGGGCCGGATCCGACGTCGGAATTTGAGCATTCGTCAATTCCTGCAACGGTGAAGAAAATTTTTGGTCTGAAACAGTTCTTGACGAAGCGTGACCAATGGGCCGGCACCTTTGACATTGTTCTTAATCGCCACACCCCTAGAACTGATTGTCCAGGTTTGTTTTTATGCTCCTTCTCCACAATATTATATGTCCCATATTTTTTTTGGTTAAGCTGTAAACTTCCTCCTATCCTTTTGAATAAGTTCAAATTTAATTTTATAGTTTATAATTAGAATTTAATTCTTTTTTATACAAAATATATATTTTTTTTGTAAGTATTGAACTATGAGAGGTTTTATCGAAGTATACACAACGACTCTTTGTAAAATTTTAGTTTTGAAACGGTAGGGATTCAATGTTTGTTTGTTTTTTAGAAGGTATTTAACAAATTGATGTATAAAGAAAAATACCACAGATATACATGAAAACATATGAACACACTAAAATAACTATACCCTTACTACCTATACCTATATTTCAATATATTATAGCAACTAAAATTAACATTAAGTTAAAACAATTAGCAAAATTTTGAAAGATGTTATTTTTTATATAGTATTACAAACACTTAGGAAGAGATTTGGGATGGTGTGAGTTGGTTATTAATTAAGCATGAAATTATTGTAGTCTTTATATTATAATAGTTTATGTGTCACAAATGTGTAGCTTAAAATAGTAAATTGATATTTAGGTCACTTATGTATTCATGGTATAGCTAATTATGAGTTAAAATAATAGTGTTCATTCTATCTAATAAGGGTTTAAAATAATAGTCTCTCAGTACAACTAATTTTGAGTTTAATAATTGTAACCTTTAGTTAGAATAATAATAGGTGCCAAACACCGAGAGTGGATTATAACATCTCATTTCACCCAACTTATAAAATTAGACCCCAAACACCCTCTTACATATTATATATCAATCCGCCTCTCACCACCTATCAAATTATTCCTAAAGTCTATGCAAAATGTCAGATATGTATCACTACTACGTTAATCTTAGCTTTTGTTGATGCATCACGCATGGAATATAAAAGATTATTTGTATTTGAAATCAACTTTGTGAATGATCTATAACAGTCACACTAAACAATCCTGTGAAATTACGGGACGTCGAGGCGAATGAGACGAGGCAGATCAGCGAGTTCCAGGAAGAGTTGGTACAGTTAGCAGCAGTACTAAGAGGAGATGGGAAGAAAGAAATCTATCCCAAAAAGTTAGTAGAGAAAATGTCTGTTGTGGAGGCAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCTTCAATGAGTGTGAAAAAGCTAAAGAAAATGGAGCTGATGAATCTCAAGTTGTTGTTTGTGGAAAGAACAACCAAATTCTTCAACCTTCTGATTCCAAGCCGAAATCCTTAGCTCGTAAGTTCTTTGCCTGCTTCGCTTGCCATGGTTGA

mRNA sequence

ATGTCACAAGCAAAAAGAACAAAGCATAATTGGAGCCTGAAGAATTTAAGAAAACTGAAATACATAAAGAACTTCCATATCTTCGACTTGGATTTCAAGAGAGATTGTCGAGAAGGAAAGCTACCAAACTACGTAGTGATAGAACAGAGATACTTTGATTTAGCATCACTACCTGGAAATGATGATCATCCTTCCCACGACGTTTCAGAAGGCCAAAAACTCATCAAAGAGGTCTATGAAGCTCTGCGATCCAGTCCACAGTGGAACGAAATCCTCTTCTTGATCACTTACGACGAGCATGGCGGTTTCTTCGATCACGTCCCTCCCCCCTCTGCTGGAGTTCCCAATCCTGACGGCCGACTCGGCCCTCCGCCATACAATTTCAATTTTGATCGCCTTGGAGTTCGAGTTCCGACCATCTTTGTCTCTCCTTGGATTGAACCCGGAACAGTGGTGCACAGACCGAGGGGGCCGGATCCGACGTCGGAATTTGAGCATTCGTCAATTCCTGCAACGGTGAAGAAAATTTTTGGTCTGAAACAGTTCTTGACGAAGCGTGACCAATGGGCCGGCACCTTTGACATTGTTCTTAATCGCCACACCCCTAGAACTGATTGTCCAGTCACACTAAACAATCCTGTGAAATTACGGGACGTCGAGGCGAATGAGACGAGGCAGATCAGCGAGTTCCAGGAAGAGTTGGTACAGTTAGCAGCAGTACTAAGAGGAGATGGGAAGAAAGAAATCTATCCCAAAAAGTTAGTAGAGAAAATGTCTGTTGTGGAGGCAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCTTCAATGAGTGTGAAAAAGCTAAAGAAAATGGAGCTGATGAATCTCAAGTTGTTGTTTGTGGAAAGAACAACCAAATTCTTCAACCTTCTGATTCCAAGCCGAAATCCTTAGCTCGTAAGTTCTTTGCCTGCTTCGCTTGCCATGGTTGA

Coding sequence (CDS)

ATGTCACAAGCAAAAAGAACAAAGCATAATTGGAGCCTGAAGAATTTAAGAAAACTGAAATACATAAAGAACTTCCATATCTTCGACTTGGATTTCAAGAGAGATTGTCGAGAAGGAAAGCTACCAAACTACGTAGTGATAGAACAGAGATACTTTGATTTAGCATCACTACCTGGAAATGATGATCATCCTTCCCACGACGTTTCAGAAGGCCAAAAACTCATCAAAGAGGTCTATGAAGCTCTGCGATCCAGTCCACAGTGGAACGAAATCCTCTTCTTGATCACTTACGACGAGCATGGCGGTTTCTTCGATCACGTCCCTCCCCCCTCTGCTGGAGTTCCCAATCCTGACGGCCGACTCGGCCCTCCGCCATACAATTTCAATTTTGATCGCCTTGGAGTTCGAGTTCCGACCATCTTTGTCTCTCCTTGGATTGAACCCGGAACAGTGGTGCACAGACCGAGGGGGCCGGATCCGACGTCGGAATTTGAGCATTCGTCAATTCCTGCAACGGTGAAGAAAATTTTTGGTCTGAAACAGTTCTTGACGAAGCGTGACCAATGGGCCGGCACCTTTGACATTGTTCTTAATCGCCACACCCCTAGAACTGATTGTCCAGTCACACTAAACAATCCTGTGAAATTACGGGACGTCGAGGCGAATGAGACGAGGCAGATCAGCGAGTTCCAGGAAGAGTTGGTACAGTTAGCAGCAGTACTAAGAGGAGATGGGAAGAAAGAAATCTATCCCAAAAAGTTAGTAGAGAAAATGTCTGTTGTGGAGGCAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCTTCAATGAGTGTGAAAAAGCTAAAGAAAATGGAGCTGATGAATCTCAAGTTGTTGTTTGTGGAAAGAACAACCAAATTCTTCAACCTTCTGATTCCAAGCCGAAATCCTTAGCTCGTAAGTTCTTTGCCTGCTTCGCTTGCCATGGTTGA
Homology
[Close]
BLAST of CSPI02G08660.1 vs. Swiss-Prot
Match: NPC4_ARATH (Non-specific phospholipase C4 OS=Arabidopsis thaliana GN=NPC4 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.4e-114
Identity = 197/323 (60.99%), Postives = 239/323 (73.99%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+ +FH + + FK+DC+EGKLPNYVV+EQR+FDL S P NDDHPSHDVSEGQK
Sbjct: 215 RNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQK 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 275 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP P S++EHSSIPATVK IF LK FL+KRD WAGTF
Sbjct: 335 GVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTF 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R +PR DCP TL+ P+KLR   A E  Q+SEFQE+LV +AA L+GD K E    K
Sbjct: 395 ESVITRDSPRQDCPETLSTPIKLRGTMAKENAQLSEFQEDLVIMAAGLKGDYKNEELIHK 454

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC--GKNNQILQPSDS----- 313
           L ++  V +A+ Y  NA + F  E  KA++ G DE+ +V C    ++ ++ P  S     
Sbjct: 455 LCKETCVADASKYVTNAFEKFLEESRKARDRGCDENDIVYCVDDDDDHVVIPPQSHSEAS 514

Query: 314 ----KPK---SLARKFFACFACH 323
               +PK   S   K F+CF  H
Sbjct: 515 NAAAQPKTQTSFFNKLFSCFIRH 537

[Close]
BLAST of CSPI02G08660.1 vs. Swiss-Prot
Match: NPC3_ARATH (Non-specific phospholipase C3 OS=Arabidopsis thaliana GN=NPC3 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 7.1e-114
Identity = 191/311 (61.41%), Postives = 233/311 (74.92%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +N+RKLKY+ NFH + L FKR C+EGKLPNYVVIE RYF + S P NDDHP +DV EGQ 
Sbjct: 215 RNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQN 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KE+YEALR+SPQWNEILF++ YDEHGG++DHVP P  GVPNPDG +GP PYNF FDRL
Sbjct: 275 LVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP + +SPWIEPGTV+H P GP+PTS+FEHSSIPAT+KKIF LK FLTKRD+WAGT 
Sbjct: 335 GVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTL 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVE---ANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           D V+NR +PRTDCPVTL    + RD++     E   +++FQ EL+Q AAVL+GD  K+IY
Sbjct: 395 DAVINRTSPRTDCPVTLPELPRARDIDIGTQEEDEDLTDFQIELIQAAAVLKGDHIKDIY 454

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKS 313
           P KL +KM V++AA Y E A   F  E +KAKE G DE ++V   K +       S PKS
Sbjct: 455 PFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRDEHEIVDLSKGS----TRHSTPKS 514

Query: 314 LARKFFACFAC 322
             +K F+C  C
Sbjct: 515 FVQKLFSCLIC 521

[Close]
BLAST of CSPI02G08660.1 vs. Swiss-Prot
Match: NPC5_ARATH (Non-specific phospholipase C5 OS=Arabidopsis thaliana GN=NPC5 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-109
Identity = 188/297 (63.30%), Postives = 228/297 (76.77%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+  FH + L FK+DC+EG LPNYVV+EQR++DL   P NDDHPSHDVSEGQK
Sbjct: 214 RNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVVVEQRWYDLLLNPANDDHPSHDVSEGQK 273

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 274 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPLDGVPNPDGILGPPPYNFEFNRL 333

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H   GP   S++EHSSIPATVKKIF LK FLTKRD WAGTF
Sbjct: 334 GVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEHSSIPATVKKIFKLKDFLTKRDSWAGTF 393

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R++PR DCP TL+NPVK+R   A E  ++S+FQEELV +AA L+GD K E    K
Sbjct: 394 ESVITRNSPRQDCPETLSNPVKMRGTVAKENAELSDFQEELVIVAAGLKGDYKNEELLYK 453

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC---GKNNQILQPSDSK 308
           L +K  V +A+ Y   A   F  E +KA+E G DE+ +V C     ++ +++P  S+
Sbjct: 454 LCKKTCVSDASKYVTKAFDKFVEESKKARERGGDENDIVFCVDDDDDHNVVKPPPSQ 510

[Close]
BLAST of CSPI02G08660.1 vs. Swiss-Prot
Match: NPC2_ARATH (Non-specific phospholipase C2 OS=Arabidopsis thaliana GN=NPC2 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.5e-92
Identity = 168/282 (59.57%), Postives = 207/282 (73.40%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           ++LRKLKY+  FH +   FK   ++GKLP Y VIEQRY D    P +DDHPSHDV +GQK
Sbjct: 222 QSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQGQK 281

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYE LR+SPQWNE L +ITYDEHGG+FDHVP P   VP+PDG +GP P+ F F+RL
Sbjct: 282 FIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFNRL 341

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRG-PDPTSEFEHSSIPATVKKIFGLKQ-FLTKRDQWAG 193
           G+RVPTI VSPWIE GTVVH P G P P+SE+EHSSIPATVKK+F L   FLTKRD+WAG
Sbjct: 342 GIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEWAG 401

Query: 194 TFDIVLN-RHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           TF+ +L  R  PRTDCP TL  PVK+R  EANE   ++EFQ+ELVQLAAVL+GD     +
Sbjct: 402 TFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLTTF 461

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           PK++ + M+V+E   Y E+A+K F      A   GA++ ++V
Sbjct: 462 PKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELV 503

[Close]
BLAST of CSPI02G08660.1 vs. Swiss-Prot
Match: NPC6_ARATH (Non-specific phospholipase C6 OS=Arabidopsis thaliana GN=NPC6 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.4e-92
Identity = 167/281 (59.43%), Postives = 203/281 (72.24%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLR+LKYI N H +DL FK+D  +GKLP+  VIE RYFDL  LP NDDHPSHDV+ GQK
Sbjct: 228 RNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQK 287

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNE L +ITYDEHGGF+DHV  P  G+PNPDG  GP P  F FDRL
Sbjct: 288 LVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRL 347

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGL-KQFLTKRDQWAGT 193
           GVRVPTI VSPWI+ GTVV   +GP  +SE+EHSSIPAT+KK+F L   FLT RD WA T
Sbjct: 348 GVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAAT 407

Query: 194 F-DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYP 253
           F D+V +  TPRTDCP+TL     +R  E  E   +SEFQ E+VQLAAVL GD     +P
Sbjct: 408 FEDVVSHLTTPRTDCPMTLPEVAPMRATEPKEDAALSEFQGEVVQLAAVLNGDHFLSSFP 467

Query: 254 KKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           +++ +KM+V +A  Y + A   F    ++A + GAD+S +V
Sbjct: 468 EEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADKSAIV 508

[Close]
BLAST of CSPI02G08660.1 vs. TrEMBL
Match: A0A0A0LN71_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G119380 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 2.3e-180
Identity = 305/309 (98.71%), Postives = 307/309 (99.35%), Query Frame = 1 

Query: 15  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 74
           NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL
Sbjct: 61  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 120

Query: 75  IKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 134
           IKEVYEALRS PQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG
Sbjct: 121 IKEVYEALRSCPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 180

Query: 135 VRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 194
           +RVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD
Sbjct: 181 LRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 240

Query: 195 IVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKKL 254
           IVLNRHTPRTDCPVTLNNPVKLRDVEANE RQISEFQEELVQLAAVLRGDGKKEIYPKKL
Sbjct: 241 IVLNRHTPRTDCPVTLNNPVKLRDVEANEMRQISEFQEELVQLAAVLRGDGKKEIYPKKL 300

Query: 255 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLARK 314
           VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSLARK
Sbjct: 301 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSLARK 360

Query: 315 FFACFACHG 324
           FFACFACHG
Sbjct: 361 FFACFACHG 369

[Close]
BLAST of CSPI02G08660.1 vs. TrEMBL
Match: A0A061F088_THECC (Non-specific phospholipase C4 OS=Theobroma cacao GN=TCM_025686 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.4e-124
Identity = 212/306 (69.28%), Postives = 244/306 (79.74%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+KNFH FDL FK  C EGKLPNYVV+EQRYFDL S+P NDDHPSHDVSEGQK
Sbjct: 212 RNLRKLKYLKNFHQFDLHFKEHCEEGKLPNYVVVEQRYFDLLSVPANDDHPSHDVSEGQK 271

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALR+SPQWNE+L +I YDEHGGF+DHVP P+ GVP+PD  +GP PY F FDRL
Sbjct: 272 FVKEVYEALRASPQWNEMLLVIAYDEHGGFYDHVPTPTIGVPSPDDIIGPEPYYFKFDRL 331

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP PTSEFEHSSIPATVK IF LK+FLTKRD WAGTF
Sbjct: 332 GVRVPTFFISPWIEPGTVIHGPSGPYPTSEFEHSSIPATVKTIFNLKEFLTKRDAWAGTF 391

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+NR  PRTDCPVTL  PVK+RD  A ET ++SEFQ+ELVQ+AAVL GD KK+IYP K
Sbjct: 392 ECVINRENPRTDCPVTLTEPVKMRDAAAKETAKLSEFQQELVQMAAVLSGDHKKKIYPHK 451

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVE M+V EA  Y + A K F +EC+KAKE G DES++V  G+      P     KS+ R
Sbjct: 452 LVENMTVAEAVKYVDGAFKKFCHECQKAKERGIDESEIVDLGE-----LPQRLNSKSVVR 511

Query: 314 KFFACF 320
           K F+CF
Sbjct: 512 KMFSCF 512

[Close]
BLAST of CSPI02G08660.1 vs. TrEMBL
Match: M5X2R5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004364mg PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.8e-122
Identity = 211/308 (68.51%), Postives = 247/308 (80.19%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYIKNFH FDL FK+ C EGKLPNYVV+EQR+FD+ S P NDDHPSHDVSEGQK
Sbjct: 210 RNLRKLKYIKNFHQFDLQFKKHCEEGKLPNYVVVEQRFFDVLSFPANDDHPSHDVSEGQK 269

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYEALR+SPQWNE+LF+I YDEHGGF+DHVP P   VP+PD   GP PYNF FDRL
Sbjct: 270 FIKEVYEALRASPQWNEMLFVIIYDEHGGFYDHVPTPVTDVPSPDDIAGPEPYNFKFDRL 329

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP I +SPWIE GTV+H P GP P+SEFEHSSIPATVKKIF LK+FLTKRD WAGTF
Sbjct: 330 GVRVPAILISPWIERGTVLHGPSGPYPSSEFEHSSIPATVKKIFNLKEFLTKRDAWAGTF 389

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + VL+R TPRTDCPVTL  PVKLR+  + +  ++S+FQEE+VQLAA + GD +K+IYP K
Sbjct: 390 EGVLSRSTPRTDCPVTLPEPVKLRETTSKDGAKMSDFQEEVVQLAAAINGDHRKDIYPNK 449

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVEKMSV +A SY E + K F +ECEKA+E+GADE ++VVC     +  PS   PKS A+
Sbjct: 450 LVEKMSVSKAVSYVEASFKKFSDECEKARESGADEYEIVVCA--TSLGSPS---PKSFAK 509

Query: 314 KFFACFAC 322
           K F+C  C
Sbjct: 510 KMFSCLVC 512

[Close]
BLAST of CSPI02G08660.1 vs. TrEMBL
Match: A0A0K9QM76_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_169900 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.4e-121
Identity = 208/309 (67.31%), Postives = 250/309 (80.91%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+  FH FDL+FK+ C+EGKLPNYVVIEQRYFDL  +PGNDDHPSHDVS GQK
Sbjct: 210 RNLRKLKYVSRFHQFDLEFKKHCKEGKLPNYVVIEQRYFDLLVVPGNDDHPSHDVSIGQK 269

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVP+PDG +GP P++F FDRL
Sbjct: 270 FIKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPVEGVPSPDGIVGPQPFSFGFDRL 329

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPTIF+SPWI+PGTV+H P GP PTSE+EHSSIPATVKKIF LK FLTKRD WAGTF
Sbjct: 330 GVRVPTIFISPWIQPGTVLHGPSGPYPTSEYEHSSIPATVKKIFKLKDFLTKRDAWAGTF 389

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           ++V+NR TPRTDCPVTL  PVKLR   A E +QIS+FQ ELVQ+AA L+GD KK+ YP +
Sbjct: 390 EVVVNRETPRTDCPVTLPEPVKLRSSLAQENKQISDFQAELVQMAACLKGDHKKKTYPYE 449

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVEKM+V EA  Y ++A K F +EC+++++ G DES +V+      +  P +S+ +S   
Sbjct: 450 LVEKMTVKEAVEYVQDAWKKFLHECDESRKRGEDESTIVI------VTSPKNSR-RSFKG 509

Query: 314 KFFACFACH 323
           K  +C AC+
Sbjct: 510 KLLSCLACN 511

[Close]
BLAST of CSPI02G08660.1 vs. TrEMBL
Match: V4TBG2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031213mg PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-121
Identity = 212/310 (68.39%), Postives = 246/310 (79.35%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+K+FH FDL FK+DC EGKLPNYVV+EQRYFDL S+P NDDHPSHDVSEGQK
Sbjct: 224 RNLRKLKYLKHFHQFDLHFKKDCEEGKLPNYVVVEQRYFDLLSVPANDDHPSHDVSEGQK 283

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALRSSPQWNEILF+ITYDEHGGF+DHV  P  GVP+PD  +GP PYNF FDRL
Sbjct: 284 FVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVQTPVTGVPSPDDIVGPEPYNFKFDRL 343

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRV TIF+SPWI+ GT++H P GP PTSEFEHSSIPATVKKIF LK+FLTKRD WAGTF
Sbjct: 344 GVRVLTIFISPWIQRGTLLHGPSGPAPTSEFEHSSIPATVKKIFNLKEFLTKRDAWAGTF 403

Query: 194 DIVLNRHTPRTDCPVTLNNP-VKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPK 253
           + VLNR +PRTDCPVTL  P +KLRD EA E  ++++FQ+ELVQ+AA L GD KK+IYP 
Sbjct: 404 ESVLNRTSPRTDCPVTLPEPTLKLRDGEAKEDAKLTDFQQELVQMAATLNGDYKKDIYPH 463

Query: 254 KLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLA 313
           KLVE+M+V E A Y E+A K F  +CEKA+  G DESQ+VV             K KS  
Sbjct: 464 KLVEEMTVAEGAKYVEDAFKKFRGQCEKAEAEGVDESQIVVVPTPT----TKQRKSKSFG 523

Query: 314 RKFFACFACH 323
           RK  +C AC+
Sbjct: 524 RKLLSCLACN 529

[Close]
BLAST of CSPI02G08660.1 vs. TAIR10
Match: AT3G03530.1 (AT3G03530.1 non-specific phospholipase C4)

HSP 1 Score: 412.1 bits (1058), Expect = 3.1e-115
Identity = 197/323 (60.99%), Postives = 239/323 (73.99%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+ +FH + + FK+DC+EGKLPNYVV+EQR+FDL S P NDDHPSHDVSEGQK
Sbjct: 215 RNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQK 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 275 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP P S++EHSSIPATVK IF LK FL+KRD WAGTF
Sbjct: 335 GVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTF 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R +PR DCP TL+ P+KLR   A E  Q+SEFQE+LV +AA L+GD K E    K
Sbjct: 395 ESVITRDSPRQDCPETLSTPIKLRGTMAKENAQLSEFQEDLVIMAAGLKGDYKNEELIHK 454

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC--GKNNQILQPSDS----- 313
           L ++  V +A+ Y  NA + F  E  KA++ G DE+ +V C    ++ ++ P  S     
Sbjct: 455 LCKETCVADASKYVTNAFEKFLEESRKARDRGCDENDIVYCVDDDDDHVVIPPQSHSEAS 514

Query: 314 ----KPK---SLARKFFACFACH 323
               +PK   S   K F+CF  H
Sbjct: 515 NAAAQPKTQTSFFNKLFSCFIRH 537

[Close]
BLAST of CSPI02G08660.1 vs. TAIR10
Match: AT3G03520.1 (AT3G03520.1 non-specific phospholipase C3)

HSP 1 Score: 411.8 bits (1057), Expect = 4.0e-115
Identity = 191/311 (61.41%), Postives = 233/311 (74.92%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +N+RKLKY+ NFH + L FKR C+EGKLPNYVVIE RYF + S P NDDHP +DV EGQ 
Sbjct: 215 RNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQN 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KE+YEALR+SPQWNEILF++ YDEHGG++DHVP P  GVPNPDG +GP PYNF FDRL
Sbjct: 275 LVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP + +SPWIEPGTV+H P GP+PTS+FEHSSIPAT+KKIF LK FLTKRD+WAGT 
Sbjct: 335 GVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTL 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVE---ANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           D V+NR +PRTDCPVTL    + RD++     E   +++FQ EL+Q AAVL+GD  K+IY
Sbjct: 395 DAVINRTSPRTDCPVTLPELPRARDIDIGTQEEDEDLTDFQIELIQAAAVLKGDHIKDIY 454

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKS 313
           P KL +KM V++AA Y E A   F  E +KAKE G DE ++V   K +       S PKS
Sbjct: 455 PFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRDEHEIVDLSKGS----TRHSTPKS 514

Query: 314 LARKFFACFAC 322
             +K F+C  C
Sbjct: 515 FVQKLFSCLIC 521

[Close]
BLAST of CSPI02G08660.1 vs. TAIR10
Match: AT3G03540.1 (AT3G03540.1 non-specific phospholipase C5)

HSP 1 Score: 397.9 bits (1021), Expect = 6.0e-111
Identity = 188/297 (63.30%), Postives = 228/297 (76.77%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+  FH + L FK+DC+EG LPNYVV+EQR++DL   P NDDHPSHDVSEGQK
Sbjct: 214 RNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVVVEQRWYDLLLNPANDDHPSHDVSEGQK 273

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 274 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPLDGVPNPDGILGPPPYNFEFNRL 333

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H   GP   S++EHSSIPATVKKIF LK FLTKRD WAGTF
Sbjct: 334 GVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEHSSIPATVKKIFKLKDFLTKRDSWAGTF 393

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R++PR DCP TL+NPVK+R   A E  ++S+FQEELV +AA L+GD K E    K
Sbjct: 394 ESVITRNSPRQDCPETLSNPVKMRGTVAKENAELSDFQEELVIVAAGLKGDYKNEELLYK 453

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC---GKNNQILQPSDSK 308
           L +K  V +A+ Y   A   F  E +KA+E G DE+ +V C     ++ +++P  S+
Sbjct: 454 LCKKTCVSDASKYVTKAFDKFVEESKKARERGGDENDIVFCVDDDDDHNVVKPPPSQ 510

[Close]
BLAST of CSPI02G08660.1 vs. TAIR10
Match: AT2G26870.1 (AT2G26870.1 non-specific phospholipase C2)

HSP 1 Score: 340.9 bits (873), Expect = 8.7e-94
Identity = 168/282 (59.57%), Postives = 207/282 (73.40%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           ++LRKLKY+  FH +   FK   ++GKLP Y VIEQRY D    P +DDHPSHDV +GQK
Sbjct: 222 QSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQGQK 281

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYE LR+SPQWNE L +ITYDEHGG+FDHVP P   VP+PDG +GP P+ F F+RL
Sbjct: 282 FIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFNRL 341

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRG-PDPTSEFEHSSIPATVKKIFGLKQ-FLTKRDQWAG 193
           G+RVPTI VSPWIE GTVVH P G P P+SE+EHSSIPATVKK+F L   FLTKRD+WAG
Sbjct: 342 GIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEWAG 401

Query: 194 TFDIVLN-RHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           TF+ +L  R  PRTDCP TL  PVK+R  EANE   ++EFQ+ELVQLAAVL+GD     +
Sbjct: 402 TFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLTTF 461

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           PK++ + M+V+E   Y E+A+K F      A   GA++ ++V
Sbjct: 462 PKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELV 503

[Close]
BLAST of CSPI02G08660.1 vs. TAIR10
Match: AT3G48610.1 (AT3G48610.1 non-specific phospholipase C6)

HSP 1 Score: 339.7 bits (870), Expect = 1.9e-93
Identity = 167/281 (59.43%), Postives = 203/281 (72.24%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLR+LKYI N H +DL FK+D  +GKLP+  VIE RYFDL  LP NDDHPSHDV+ GQK
Sbjct: 228 RNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQK 287

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNE L +ITYDEHGGF+DHV  P  G+PNPDG  GP P  F FDRL
Sbjct: 288 LVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRL 347

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGL-KQFLTKRDQWAGT 193
           GVRVPTI VSPWI+ GTVV   +GP  +SE+EHSSIPAT+KK+F L   FLT RD WA T
Sbjct: 348 GVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAAT 407

Query: 194 F-DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYP 253
           F D+V +  TPRTDCP+TL     +R  E  E   +SEFQ E+VQLAAVL GD     +P
Sbjct: 408 FEDVVSHLTTPRTDCPMTLPEVAPMRATEPKEDAALSEFQGEVVQLAAVLNGDHFLSSFP 467

Query: 254 KKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           +++ +KM+V +A  Y + A   F    ++A + GAD+S +V
Sbjct: 468 EEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADKSAIV 508

[Close]
BLAST of CSPI02G08660.1 vs. NCBI nr
Match: gi|449442455|ref|XP_004138997.1| (PREDICTED: non-specific phospholipase C3-like [Cucumis sativus])

HSP 1 Score: 640.2 bits (1650), Expect = 2.0e-180
Identity = 305/310 (98.39%), Postives = 308/310 (99.35%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK
Sbjct: 220 RNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 279

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           LIKEVYEALRS PQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL
Sbjct: 280 LIKEVYEALRSCPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 339

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           G+RVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF
Sbjct: 340 GLRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 399

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           DIVLNRHTPRTDCPVTLNNPVKLRDVEANE RQISEFQEELVQLAAVLRGDGKKEIYPKK
Sbjct: 400 DIVLNRHTPRTDCPVTLNNPVKLRDVEANEMRQISEFQEELVQLAAVLRGDGKKEIYPKK 459

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSLAR
Sbjct: 460 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSLAR 519

Query: 314 KFFACFACHG 324
           KFFACFACHG
Sbjct: 520 KFFACFACHG 529

[Close]
BLAST of CSPI02G08660.1 vs. NCBI nr
Match: gi|700206297|gb|KGN61416.1| (hypothetical protein Csa_2G119380 [Cucumis sativus])

HSP 1 Score: 639.4 bits (1648), Expect = 3.3e-180
Identity = 305/309 (98.71%), Postives = 307/309 (99.35%), Query Frame = 1 

Query: 15  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 74
           NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL
Sbjct: 61  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 120

Query: 75  IKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 134
           IKEVYEALRS PQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG
Sbjct: 121 IKEVYEALRSCPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 180

Query: 135 VRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 194
           +RVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD
Sbjct: 181 LRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 240

Query: 195 IVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKKL 254
           IVLNRHTPRTDCPVTLNNPVKLRDVEANE RQISEFQEELVQLAAVLRGDGKKEIYPKKL
Sbjct: 241 IVLNRHTPRTDCPVTLNNPVKLRDVEANEMRQISEFQEELVQLAAVLRGDGKKEIYPKKL 300

Query: 255 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLARK 314
           VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSLARK
Sbjct: 301 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSLARK 360

Query: 315 FFACFACHG 324
           FFACFACHG
Sbjct: 361 FFACFACHG 369

[Close]
BLAST of CSPI02G08660.1 vs. NCBI nr
Match: gi|659114666|ref|XP_008457171.1| (PREDICTED: non-specific phospholipase C3-like [Cucumis melo])

HSP 1 Score: 594.3 bits (1531), Expect = 1.2e-166
Identity = 285/298 (95.64%), Postives = 291/298 (97.65%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYI NFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK
Sbjct: 212 RNLRKLKYIMNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 271

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPD RLGPPPYNFNFDRL
Sbjct: 272 FIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDARLGPPPYNFNFDRL 331

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPTIFVSPWIEPGTVVHRP GPDPTSEFEHSSI ATVKKIFGLK+FLTKRDQWAGTF
Sbjct: 332 GVRVPTIFVSPWIEPGTVVHRPTGPDPTSEFEHSSIAATVKKIFGLKEFLTKRDQWAGTF 391

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           DIVLNRHTPRTDCPVTLNNPVKLR+VEAN+ RQISEFQEELVQLAAVL+GD KKEIYPKK
Sbjct: 392 DIVLNRHTPRTDCPVTLNNPVKLRNVEANDRRQISEFQEELVQLAAVLKGDVKKEIYPKK 451

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSL 312
           LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSL
Sbjct: 452 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSL 509

[Close]
BLAST of CSPI02G08660.1 vs. NCBI nr
Match: gi|720054739|ref|XP_010273152.1| (PREDICTED: non-specific phospholipase C4-like isoform X2 [Nelumbo nucifera])

HSP 1 Score: 456.1 bits (1172), Expect = 5.3e-125
Identity = 218/315 (69.21%), Postives = 255/315 (80.95%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYIKNFH FD++FK  C+EGKLPNYVV+EQRYFDL  LPGNDDHPSHDVSEGQK
Sbjct: 210 RNLRKLKYIKNFHQFDMNFKSHCKEGKLPNYVVVEQRYFDLKLLPGNDDHPSHDVSEGQK 269

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALR+SPQWNEILF+ITYDEHGGF+DHVP P+ GVP+PD    P P+ F FDRL
Sbjct: 270 FVKEVYEALRASPQWNEILFIITYDEHGGFYDHVPTPT-GVPSPDDIASPGPFYFKFDRL 329

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP IF+SPWIE GTV+HRP GP P+SEFEHSSIPATVKKIF LK+FLTKRD WAGTF
Sbjct: 330 GVRVPAIFISPWIERGTVLHRPSGPYPSSEFEHSSIPATVKKIFNLKEFLTKRDAWAGTF 389

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + VL R +PRTDCPVTL  P KLR  +ANE  ++SEFQ ELVQLAA L+GD +K+IYP K
Sbjct: 390 ETVLTRESPRTDCPVTLPQPRKLRSGDANEGAKLSEFQVELVQLAAALKGDHRKDIYPNK 449

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC-----GKNN-QILQPSDSK 313
           LVEKM+V +A SYC++A K F   CEKAK+NGADES+++V       KN+  +++    K
Sbjct: 450 LVEKMTVKDAVSYCKDAFKKFLEGCEKAKKNGADESEIIVLPSLPPSKNSTPVVEQPPPK 509

Query: 314 PKSLARKFFACFACH 323
            KS   K FACFAC+
Sbjct: 510 SKSFVHKMFACFACN 523

[Close]
BLAST of CSPI02G08660.1 vs. NCBI nr
Match: gi|590639930|ref|XP_007029811.1| (Non-specific phospholipase C4 [Theobroma cacao])

HSP 1 Score: 454.1 bits (1167), Expect = 2.0e-124
Identity = 212/306 (69.28%), Postives = 244/306 (79.74%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+KNFH FDL FK  C EGKLPNYVV+EQRYFDL S+P NDDHPSHDVSEGQK
Sbjct: 212 RNLRKLKYLKNFHQFDLHFKEHCEEGKLPNYVVVEQRYFDLLSVPANDDHPSHDVSEGQK 271

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALR+SPQWNE+L +I YDEHGGF+DHVP P+ GVP+PD  +GP PY F FDRL
Sbjct: 272 FVKEVYEALRASPQWNEMLLVIAYDEHGGFYDHVPTPTIGVPSPDDIIGPEPYYFKFDRL 331

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP PTSEFEHSSIPATVK IF LK+FLTKRD WAGTF
Sbjct: 332 GVRVPTFFISPWIEPGTVIHGPSGPYPTSEFEHSSIPATVKTIFNLKEFLTKRDAWAGTF 391

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+NR  PRTDCPVTL  PVK+RD  A ET ++SEFQ+ELVQ+AAVL GD KK+IYP K
Sbjct: 392 ECVINRENPRTDCPVTLTEPVKMRDAAAKETAKLSEFQQELVQMAAVLSGDHKKKIYPHK 451

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVE M+V EA  Y + A K F +EC+KAKE G DES++V  G+      P     KS+ R
Sbjct: 452 LVENMTVAEAVKYVDGAFKKFCHECQKAKERGIDESEIVDLGE-----LPQRLNSKSVVR 511

Query: 314 KFFACF 320
           K F+CF
Sbjct: 512 KMFSCF 512

The following BLAST results are available for this feature:
BLAST of CSPI02G08660.1 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NPC4_ARATH5.4e-11460.99Non-specific phospholipase C4 OS=Arabidopsis thaliana GN=NPC4 PE=1 SV=1[more]
NPC3_ARATH7.1e-11461.41Non-specific phospholipase C3 OS=Arabidopsis thaliana GN=NPC3 PE=2 SV=1[more]
NPC5_ARATH1.1e-10963.30Non-specific phospholipase C5 OS=Arabidopsis thaliana GN=NPC5 PE=2 SV=1[more]
NPC2_ARATH1.5e-9259.57Non-specific phospholipase C2 OS=Arabidopsis thaliana GN=NPC2 PE=2 SV=1[more]
NPC6_ARATH3.4e-9259.43Non-specific phospholipase C6 OS=Arabidopsis thaliana GN=NPC6 PE=2 SV=1[more]
BLAST of CSPI02G08660.1 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LN71_CUCSA2.3e-18098.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G119380 PE=4 SV=1[more]
A0A061F088_THECC1.4e-12469.28Non-specific phospholipase C4 OS=Theobroma cacao GN=TCM_025686 PE=4 SV=1[more]
M5X2R5_PRUPE3.8e-12268.51Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004364mg PE=4 SV=1[more]
A0A0K9QM76_SPIOL1.4e-12167.31Uncharacterized protein OS=Spinacia oleracea GN=SOVF_169900 PE=4 SV=1[more]
V4TBG2_9ROSI2.5e-12168.39Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031213mg PE=4 SV=1[more]
BLAST of CSPI02G08660.1 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G03530.13.1e-11560.99 non-specific phospholipase C4[more]
AT3G03520.14.0e-11561.41 non-specific phospholipase C3[more]
AT3G03540.16.0e-11163.30 non-specific phospholipase C5[more]
AT2G26870.18.7e-9459.57 non-specific phospholipase C2[more]
AT3G48610.11.9e-9359.43 non-specific phospholipase C6[more]
BLAST of CSPI02G08660.1 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442455|ref|XP_004138997.1|2.0e-18098.39PREDICTED: non-specific phospholipase C3-like [Cucumis sativus][more]
gi|700206297|gb|KGN61416.1|3.3e-18098.71hypothetical protein Csa_2G119380 [Cucumis sativus][more]
gi|659114666|ref|XP_008457171.1|1.2e-16695.64PREDICTED: non-specific phospholipase C3-like [Cucumis melo][more]
gi|720054739|ref|XP_010273152.1|5.3e-12569.21PREDICTED: non-specific phospholipase C4-like isoform X2 [Nelumbo nucifera][more]
gi|590639930|ref|XP_007029811.1|2.0e-12469.28Non-specific phospholipase C4 [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR007312Phosphoesterase
IPR017850Alkaline_phosphatase_core_sf
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI02G08660CSPI02G08660gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI02G08660.1CSPI02G08660.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI02G08660.1.cds1CSPI02G08660.1.cds1CDS
CSPI02G08660.1.cds2CSPI02G08660.1.cds2CDS
CSPI02G08660.1.cds3CSPI02G08660.1.cds3CDS
CSPI02G08660.1.cds4CSPI02G08660.1.cds4CDS


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007312PhosphoesterasePFAMPF04185Phosphoesterasecoord: 13..180
score: 1.7
IPR017850Alkaline-phosphatase-like, core domainunknownSSF53649Alkaline phosphatase-likecoord: 74..181
score: 1.3
NoneNo IPR availablePANTHERPTHR31956FAMILY NOT NAMEDcoord: 15..310
score: 5.1E
NoneNo IPR availablePANTHERPTHR31956:SF1NON-SPECIFIC PHOSPHOLIPASE C3-RELATEDcoord: 15..310
score: 5.1E