CSPI02G08660 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G08660
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
Descriptionnon-specific phospholipase C4
LocationChr2 : 8206393 .. 8208937 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACAAGCAAAAAGAACAAAGCATAATTGGAGCCTGAAGTGAACCAGCAAATAGTAAATTAAACAACTATAAATTGGAACAAAATGGCAACAAAACCCTAAGAACAAAAGAGTTTTTCTCCAAATAACATCACCCGATTAGATTTGAAAGGAATCAAATACATAGACCAACCAGAAAACAAAAACTGAAACGAACTCAACCTTGTGAGAACATAGTGTCTTCAAACGAATAAGCAAATAGACGAATGTAAACTCTAGAAAGGCTTTGGTACCATGCACTTTATACTTTTGGAGAATAATAATCTTATTATATATTTTAATTAGGCTAAAGATACATATGTATAGGCGACTAAGAAACCCTACTAATCTAGGGAATATAAAATTACAATAAAGGACAACTATACATGATGATGATGATGATATAAATACAGATTATAGCAATTATATTTCCTTCCATTGTGTATTAATTTGATCTAACGTAATAACATATTATTTTTTAAAACATAGGAATTTAAGAAAACTGAAATACATAAAGAACTTCCATATCTTCGACTTGGATTTCAAGAGAGATTGTCGAGAAGGAAAGCTACCAAACTACGTAGTGATAGAACAGAGATACTTTGATTTAGCATCACTACCTGGAAATGATGATCATCCTTCCCACGACGTTTCAGAAGGCCAAAAACTCATCAAAGAGGTCTATGAAGCTCTGCGATCCAGTCCACAGTGGAACGAAATCCTCTTCTTGATCACTTACGACGAGCATGGCGGTTTCTTCGATCACGTCCCTCCCCCCTCTGCTGGAGTTCCCAATCCTGACGGCCGACTCGGCCCTCCGCCATACAATTTCAATTTTGATCGCCTTGGAGTTCGAGTTCCGACCATCTTTGTCTCTCCTTGGATTGAACCCGGAACAGGTTTTTTCTTCTTCTTTTAAATCTGTGAATTGTTTGTTTGTTTCAGAATTTCTGGTGTTAGAAAATTTCAATCGAAGCTATTAGTACAGTTACACATAAAAAATGTAAATTTGAGAAGGATGGTGTATGTAAAATACAGTGGTGCACAGACCGAGGGGGCCGGATCCGACGTCGGAATTTGAGCATTCGTCAATTCCTGCAACGGTGAAGAAAATTTTTGGTCTGAAACAGTTCTTGACGAAGCGTGACCAATGGGCCGGCACCTTTGACATTGTTCTTAATCGCCACACCCCTAGAACTGATTGTCCAGGTTTGTTTTTATGCTCCTTCTCCACAATATTATATGTCCCATATTTTTTTTGGTTAAGCTGTAAACTTCCTCCTATCCTTTTGAATAAGTTCAAATTTAATTTTATAGTTTATAATTAGAATTTAATTCTTTTTTATACAAAATATATATTTTTTTTGTAAGTATTGAACTATGAGAGGTTTTATCGAAGTATACACAACGACTCTTTGTAAAATTTTAGTTTTGAAACGGTAGGGATTCAATGTTTGTTTGTTTTTTAGAAGGTATTTAACAAATTGATGTATAAAGAAAAATACCACAGATATACATGAAAACATATGAACACACTAAAATAACTATACCCTTACTACCTATACCTATATTTCAATATATTATAGCAACTAAAATTAACATTAAGTTAAAACAATTAGCAAAATTTTGAAAGATGTTATTTTTTATATAGTATTACAAACACTTAGGAAGAGATTTGGGATGGTGTGAGTTGGTTATTAATTAAGCATGAAATTATTGTAGTCTTTATATTATAATAGTTTATGTGTCACAAATGTGTAGCTTAAAATAGTAAATTGATATTTAGGTCACTTATGTATTCATGGTATAGCTAATTATGAGTTAAAATAATAGTGTTCATTCTATCTAATAAGGGTTTAAAATAATAGTCTCTCAGTACAACTAATTTTGAGTTTAATAATTGTAACCTTTAGTTAGAATAATAATAGGTGCCAAACACCGAGAGTGGATTATAACATCTCATTTCACCCAACTTATAAAATTAGACCCCAAACACCCTCTTACATATTATATATCAATCCGCCTCTCACCACCTATCAAATTATTCCTAAAGTCTATGCAAAATGTCAGATATGTATCACTACTACGTTAATCTTAGCTTTTGTTGATGCATCACGCATGGAATATAAAAGATTATTTGTATTTGAAATCAACTTTGTGAATGATCTATAACAGTCACACTAAACAATCCTGTGAAATTACGGGACGTCGAGGCGAATGAGACGAGGCAGATCAGCGAGTTCCAGGAAGAGTTGGTACAGTTAGCAGCAGTACTAAGAGGAGATGGGAAGAAAGAAATCTATCCCAAAAAGTTAGTAGAGAAAATGTCTGTTGTGGAGGCAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCTTCAATGAGTGTGAAAAAGCTAAAGAAAATGGAGCTGATGAATCTCAAGTTGTTGTTTGTGGAAAGAACAACCAAATTCTTCAACCTTCTGATTCCAAGCCGAAATCCTTAGCTCGTAAGTTCTTTGCCTGCTTCGCTTGCCATGGTTGA

mRNA sequence

ATGTCACAAGCAAAAAGAACAAAGCATAATTGGAGCCTGAAGAATTTAAGAAAACTGAAATACATAAAGAACTTCCATATCTTCGACTTGGATTTCAAGAGAGATTGTCGAGAAGGAAAGCTACCAAACTACGTAGTGATAGAACAGAGATACTTTGATTTAGCATCACTACCTGGAAATGATGATCATCCTTCCCACGACGTTTCAGAAGGCCAAAAACTCATCAAAGAGGTCTATGAAGCTCTGCGATCCAGTCCACAGTGGAACGAAATCCTCTTCTTGATCACTTACGACGAGCATGGCGGTTTCTTCGATCACGTCCCTCCCCCCTCTGCTGGAGTTCCCAATCCTGACGGCCGACTCGGCCCTCCGCCATACAATTTCAATTTTGATCGCCTTGGAGTTCGAGTTCCGACCATCTTTGTCTCTCCTTGGATTGAACCCGGAACAGTGGTGCACAGACCGAGGGGGCCGGATCCGACGTCGGAATTTGAGCATTCGTCAATTCCTGCAACGGTGAAGAAAATTTTTGGTCTGAAACAGTTCTTGACGAAGCGTGACCAATGGGCCGGCACCTTTGACATTGTTCTTAATCGCCACACCCCTAGAACTGATTGTCCAGTCACACTAAACAATCCTGTGAAATTACGGGACGTCGAGGCGAATGAGACGAGGCAGATCAGCGAGTTCCAGGAAGAGTTGGTACAGTTAGCAGCAGTACTAAGAGGAGATGGGAAGAAAGAAATCTATCCCAAAAAGTTAGTAGAGAAAATGTCTGTTGTGGAGGCAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCTTCAATGAGTGTGAAAAAGCTAAAGAAAATGGAGCTGATGAATCTCAAGTTGTTGTTTGTGGAAAGAACAACCAAATTCTTCAACCTTCTGATTCCAAGCCGAAATCCTTAGCTCGTAAGTTCTTTGCCTGCTTCGCTTGCCATGGTTGA

Coding sequence (CDS)

ATGTCACAAGCAAAAAGAACAAAGCATAATTGGAGCCTGAAGAATTTAAGAAAACTGAAATACATAAAGAACTTCCATATCTTCGACTTGGATTTCAAGAGAGATTGTCGAGAAGGAAAGCTACCAAACTACGTAGTGATAGAACAGAGATACTTTGATTTAGCATCACTACCTGGAAATGATGATCATCCTTCCCACGACGTTTCAGAAGGCCAAAAACTCATCAAAGAGGTCTATGAAGCTCTGCGATCCAGTCCACAGTGGAACGAAATCCTCTTCTTGATCACTTACGACGAGCATGGCGGTTTCTTCGATCACGTCCCTCCCCCCTCTGCTGGAGTTCCCAATCCTGACGGCCGACTCGGCCCTCCGCCATACAATTTCAATTTTGATCGCCTTGGAGTTCGAGTTCCGACCATCTTTGTCTCTCCTTGGATTGAACCCGGAACAGTGGTGCACAGACCGAGGGGGCCGGATCCGACGTCGGAATTTGAGCATTCGTCAATTCCTGCAACGGTGAAGAAAATTTTTGGTCTGAAACAGTTCTTGACGAAGCGTGACCAATGGGCCGGCACCTTTGACATTGTTCTTAATCGCCACACCCCTAGAACTGATTGTCCAGTCACACTAAACAATCCTGTGAAATTACGGGACGTCGAGGCGAATGAGACGAGGCAGATCAGCGAGTTCCAGGAAGAGTTGGTACAGTTAGCAGCAGTACTAAGAGGAGATGGGAAGAAAGAAATCTATCCCAAAAAGTTAGTAGAGAAAATGTCTGTTGTGGAGGCAGCTTCATACTGTGAAAATGCACTGAAAAGTTTCTTCAATGAGTGTGAAAAAGCTAAAGAAAATGGAGCTGATGAATCTCAAGTTGTTGTTTGTGGAAAGAACAACCAAATTCTTCAACCTTCTGATTCCAAGCCGAAATCCTTAGCTCGTAAGTTCTTTGCCTGCTTCGCTTGCCATGGTTGA
Homology
[Close]
BLAST of CSPI02G08660 vs. Swiss-Prot
Match: NPC4_ARATH (Non-specific phospholipase C4 OS=Arabidopsis thaliana GN=NPC4 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.4e-114
Identity = 197/323 (60.99%), Postives = 239/323 (73.99%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+ +FH + + FK+DC+EGKLPNYVV+EQR+FDL S P NDDHPSHDVSEGQK
Sbjct: 215 RNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQK 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 275 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP P S++EHSSIPATVK IF LK FL+KRD WAGTF
Sbjct: 335 GVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTF 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R +PR DCP TL+ P+KLR   A E  Q+SEFQE+LV +AA L+GD K E    K
Sbjct: 395 ESVITRDSPRQDCPETLSTPIKLRGTMAKENAQLSEFQEDLVIMAAGLKGDYKNEELIHK 454

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC--GKNNQILQPSDS----- 313
           L ++  V +A+ Y  NA + F  E  KA++ G DE+ +V C    ++ ++ P  S     
Sbjct: 455 LCKETCVADASKYVTNAFEKFLEESRKARDRGCDENDIVYCVDDDDDHVVIPPQSHSEAS 514

Query: 314 ----KPK---SLARKFFACFACH 323
               +PK   S   K F+CF  H
Sbjct: 515 NAAAQPKTQTSFFNKLFSCFIRH 537

[Close]
BLAST of CSPI02G08660 vs. Swiss-Prot
Match: NPC3_ARATH (Non-specific phospholipase C3 OS=Arabidopsis thaliana GN=NPC3 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 7.1e-114
Identity = 191/311 (61.41%), Postives = 233/311 (74.92%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +N+RKLKY+ NFH + L FKR C+EGKLPNYVVIE RYF + S P NDDHP +DV EGQ 
Sbjct: 215 RNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQN 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KE+YEALR+SPQWNEILF++ YDEHGG++DHVP P  GVPNPDG +GP PYNF FDRL
Sbjct: 275 LVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP + +SPWIEPGTV+H P GP+PTS+FEHSSIPAT+KKIF LK FLTKRD+WAGT 
Sbjct: 335 GVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTL 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVE---ANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           D V+NR +PRTDCPVTL    + RD++     E   +++FQ EL+Q AAVL+GD  K+IY
Sbjct: 395 DAVINRTSPRTDCPVTLPELPRARDIDIGTQEEDEDLTDFQIELIQAAAVLKGDHIKDIY 454

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKS 313
           P KL +KM V++AA Y E A   F  E +KAKE G DE ++V   K +       S PKS
Sbjct: 455 PFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRDEHEIVDLSKGS----TRHSTPKS 514

Query: 314 LARKFFACFAC 322
             +K F+C  C
Sbjct: 515 FVQKLFSCLIC 521

[Close]
BLAST of CSPI02G08660 vs. Swiss-Prot
Match: NPC5_ARATH (Non-specific phospholipase C5 OS=Arabidopsis thaliana GN=NPC5 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-109
Identity = 188/297 (63.30%), Postives = 228/297 (76.77%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+  FH + L FK+DC+EG LPNYVV+EQR++DL   P NDDHPSHDVSEGQK
Sbjct: 214 RNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVVVEQRWYDLLLNPANDDHPSHDVSEGQK 273

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 274 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPLDGVPNPDGILGPPPYNFEFNRL 333

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H   GP   S++EHSSIPATVKKIF LK FLTKRD WAGTF
Sbjct: 334 GVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEHSSIPATVKKIFKLKDFLTKRDSWAGTF 393

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R++PR DCP TL+NPVK+R   A E  ++S+FQEELV +AA L+GD K E    K
Sbjct: 394 ESVITRNSPRQDCPETLSNPVKMRGTVAKENAELSDFQEELVIVAAGLKGDYKNEELLYK 453

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC---GKNNQILQPSDSK 308
           L +K  V +A+ Y   A   F  E +KA+E G DE+ +V C     ++ +++P  S+
Sbjct: 454 LCKKTCVSDASKYVTKAFDKFVEESKKARERGGDENDIVFCVDDDDDHNVVKPPPSQ 510

[Close]
BLAST of CSPI02G08660 vs. Swiss-Prot
Match: NPC2_ARATH (Non-specific phospholipase C2 OS=Arabidopsis thaliana GN=NPC2 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.5e-92
Identity = 168/282 (59.57%), Postives = 207/282 (73.40%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           ++LRKLKY+  FH +   FK   ++GKLP Y VIEQRY D    P +DDHPSHDV +GQK
Sbjct: 222 QSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQGQK 281

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYE LR+SPQWNE L +ITYDEHGG+FDHVP P   VP+PDG +GP P+ F F+RL
Sbjct: 282 FIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFNRL 341

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRG-PDPTSEFEHSSIPATVKKIFGLKQ-FLTKRDQWAG 193
           G+RVPTI VSPWIE GTVVH P G P P+SE+EHSSIPATVKK+F L   FLTKRD+WAG
Sbjct: 342 GIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEWAG 401

Query: 194 TFDIVLN-RHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           TF+ +L  R  PRTDCP TL  PVK+R  EANE   ++EFQ+ELVQLAAVL+GD     +
Sbjct: 402 TFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLTTF 461

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           PK++ + M+V+E   Y E+A+K F      A   GA++ ++V
Sbjct: 462 PKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELV 503

[Close]
BLAST of CSPI02G08660 vs. Swiss-Prot
Match: NPC6_ARATH (Non-specific phospholipase C6 OS=Arabidopsis thaliana GN=NPC6 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.4e-92
Identity = 167/281 (59.43%), Postives = 203/281 (72.24%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLR+LKYI N H +DL FK+D  +GKLP+  VIE RYFDL  LP NDDHPSHDV+ GQK
Sbjct: 228 RNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQK 287

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNE L +ITYDEHGGF+DHV  P  G+PNPDG  GP P  F FDRL
Sbjct: 288 LVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRL 347

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGL-KQFLTKRDQWAGT 193
           GVRVPTI VSPWI+ GTVV   +GP  +SE+EHSSIPAT+KK+F L   FLT RD WA T
Sbjct: 348 GVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAAT 407

Query: 194 F-DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYP 253
           F D+V +  TPRTDCP+TL     +R  E  E   +SEFQ E+VQLAAVL GD     +P
Sbjct: 408 FEDVVSHLTTPRTDCPMTLPEVAPMRATEPKEDAALSEFQGEVVQLAAVLNGDHFLSSFP 467

Query: 254 KKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           +++ +KM+V +A  Y + A   F    ++A + GAD+S +V
Sbjct: 468 EEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADKSAIV 508

[Close]
BLAST of CSPI02G08660 vs. TrEMBL
Match: A0A0A0LN71_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G119380 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 2.3e-180
Identity = 305/309 (98.71%), Postives = 307/309 (99.35%), Query Frame = 1 

Query: 15  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 74
           NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL
Sbjct: 61  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 120

Query: 75  IKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 134
           IKEVYEALRS PQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG
Sbjct: 121 IKEVYEALRSCPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 180

Query: 135 VRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 194
           +RVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD
Sbjct: 181 LRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 240

Query: 195 IVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKKL 254
           IVLNRHTPRTDCPVTLNNPVKLRDVEANE RQISEFQEELVQLAAVLRGDGKKEIYPKKL
Sbjct: 241 IVLNRHTPRTDCPVTLNNPVKLRDVEANEMRQISEFQEELVQLAAVLRGDGKKEIYPKKL 300

Query: 255 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLARK 314
           VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSLARK
Sbjct: 301 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSLARK 360

Query: 315 FFACFACHG 324
           FFACFACHG
Sbjct: 361 FFACFACHG 369

[Close]
BLAST of CSPI02G08660 vs. TrEMBL
Match: A0A061F088_THECC (Non-specific phospholipase C4 OS=Theobroma cacao GN=TCM_025686 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.4e-124
Identity = 212/306 (69.28%), Postives = 244/306 (79.74%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+KNFH FDL FK  C EGKLPNYVV+EQRYFDL S+P NDDHPSHDVSEGQK
Sbjct: 212 RNLRKLKYLKNFHQFDLHFKEHCEEGKLPNYVVVEQRYFDLLSVPANDDHPSHDVSEGQK 271

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALR+SPQWNE+L +I YDEHGGF+DHVP P+ GVP+PD  +GP PY F FDRL
Sbjct: 272 FVKEVYEALRASPQWNEMLLVIAYDEHGGFYDHVPTPTIGVPSPDDIIGPEPYYFKFDRL 331

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP PTSEFEHSSIPATVK IF LK+FLTKRD WAGTF
Sbjct: 332 GVRVPTFFISPWIEPGTVIHGPSGPYPTSEFEHSSIPATVKTIFNLKEFLTKRDAWAGTF 391

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+NR  PRTDCPVTL  PVK+RD  A ET ++SEFQ+ELVQ+AAVL GD KK+IYP K
Sbjct: 392 ECVINRENPRTDCPVTLTEPVKMRDAAAKETAKLSEFQQELVQMAAVLSGDHKKKIYPHK 451

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVE M+V EA  Y + A K F +EC+KAKE G DES++V  G+      P     KS+ R
Sbjct: 452 LVENMTVAEAVKYVDGAFKKFCHECQKAKERGIDESEIVDLGE-----LPQRLNSKSVVR 511

Query: 314 KFFACF 320
           K F+CF
Sbjct: 512 KMFSCF 512

[Close]
BLAST of CSPI02G08660 vs. TrEMBL
Match: M5X2R5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004364mg PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.8e-122
Identity = 211/308 (68.51%), Postives = 247/308 (80.19%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYIKNFH FDL FK+ C EGKLPNYVV+EQR+FD+ S P NDDHPSHDVSEGQK
Sbjct: 210 RNLRKLKYIKNFHQFDLQFKKHCEEGKLPNYVVVEQRFFDVLSFPANDDHPSHDVSEGQK 269

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYEALR+SPQWNE+LF+I YDEHGGF+DHVP P   VP+PD   GP PYNF FDRL
Sbjct: 270 FIKEVYEALRASPQWNEMLFVIIYDEHGGFYDHVPTPVTDVPSPDDIAGPEPYNFKFDRL 329

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP I +SPWIE GTV+H P GP P+SEFEHSSIPATVKKIF LK+FLTKRD WAGTF
Sbjct: 330 GVRVPAILISPWIERGTVLHGPSGPYPSSEFEHSSIPATVKKIFNLKEFLTKRDAWAGTF 389

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + VL+R TPRTDCPVTL  PVKLR+  + +  ++S+FQEE+VQLAA + GD +K+IYP K
Sbjct: 390 EGVLSRSTPRTDCPVTLPEPVKLRETTSKDGAKMSDFQEEVVQLAAAINGDHRKDIYPNK 449

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVEKMSV +A SY E + K F +ECEKA+E+GADE ++VVC     +  PS   PKS A+
Sbjct: 450 LVEKMSVSKAVSYVEASFKKFSDECEKARESGADEYEIVVCA--TSLGSPS---PKSFAK 509

Query: 314 KFFACFAC 322
           K F+C  C
Sbjct: 510 KMFSCLVC 512

[Close]
BLAST of CSPI02G08660 vs. TrEMBL
Match: A0A0K9QM76_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_169900 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.4e-121
Identity = 208/309 (67.31%), Postives = 250/309 (80.91%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+  FH FDL+FK+ C+EGKLPNYVVIEQRYFDL  +PGNDDHPSHDVS GQK
Sbjct: 210 RNLRKLKYVSRFHQFDLEFKKHCKEGKLPNYVVIEQRYFDLLVVPGNDDHPSHDVSIGQK 269

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVP+PDG +GP P++F FDRL
Sbjct: 270 FIKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPVEGVPSPDGIVGPQPFSFGFDRL 329

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPTIF+SPWI+PGTV+H P GP PTSE+EHSSIPATVKKIF LK FLTKRD WAGTF
Sbjct: 330 GVRVPTIFISPWIQPGTVLHGPSGPYPTSEYEHSSIPATVKKIFKLKDFLTKRDAWAGTF 389

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           ++V+NR TPRTDCPVTL  PVKLR   A E +QIS+FQ ELVQ+AA L+GD KK+ YP +
Sbjct: 390 EVVVNRETPRTDCPVTLPEPVKLRSSLAQENKQISDFQAELVQMAACLKGDHKKKTYPYE 449

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVEKM+V EA  Y ++A K F +EC+++++ G DES +V+      +  P +S+ +S   
Sbjct: 450 LVEKMTVKEAVEYVQDAWKKFLHECDESRKRGEDESTIVI------VTSPKNSR-RSFKG 509

Query: 314 KFFACFACH 323
           K  +C AC+
Sbjct: 510 KLLSCLACN 511

[Close]
BLAST of CSPI02G08660 vs. TrEMBL
Match: V4TBG2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031213mg PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.5e-121
Identity = 212/310 (68.39%), Postives = 246/310 (79.35%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+K+FH FDL FK+DC EGKLPNYVV+EQRYFDL S+P NDDHPSHDVSEGQK
Sbjct: 224 RNLRKLKYLKHFHQFDLHFKKDCEEGKLPNYVVVEQRYFDLLSVPANDDHPSHDVSEGQK 283

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALRSSPQWNEILF+ITYDEHGGF+DHV  P  GVP+PD  +GP PYNF FDRL
Sbjct: 284 FVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVQTPVTGVPSPDDIVGPEPYNFKFDRL 343

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRV TIF+SPWI+ GT++H P GP PTSEFEHSSIPATVKKIF LK+FLTKRD WAGTF
Sbjct: 344 GVRVLTIFISPWIQRGTLLHGPSGPAPTSEFEHSSIPATVKKIFNLKEFLTKRDAWAGTF 403

Query: 194 DIVLNRHTPRTDCPVTLNNP-VKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPK 253
           + VLNR +PRTDCPVTL  P +KLRD EA E  ++++FQ+ELVQ+AA L GD KK+IYP 
Sbjct: 404 ESVLNRTSPRTDCPVTLPEPTLKLRDGEAKEDAKLTDFQQELVQMAATLNGDYKKDIYPH 463

Query: 254 KLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLA 313
           KLVE+M+V E A Y E+A K F  +CEKA+  G DESQ+VV             K KS  
Sbjct: 464 KLVEEMTVAEGAKYVEDAFKKFRGQCEKAEAEGVDESQIVVVPTPT----TKQRKSKSFG 523

Query: 314 RKFFACFACH 323
           RK  +C AC+
Sbjct: 524 RKLLSCLACN 529

[Close]
BLAST of CSPI02G08660 vs. TAIR10
Match: AT3G03530.1 (AT3G03530.1 non-specific phospholipase C4)

HSP 1 Score: 412.1 bits (1058), Expect = 3.1e-115
Identity = 197/323 (60.99%), Postives = 239/323 (73.99%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+ +FH + + FK+DC+EGKLPNYVV+EQR+FDL S P NDDHPSHDVSEGQK
Sbjct: 215 RNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQK 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 275 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP P S++EHSSIPATVK IF LK FL+KRD WAGTF
Sbjct: 335 GVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTF 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R +PR DCP TL+ P+KLR   A E  Q+SEFQE+LV +AA L+GD K E    K
Sbjct: 395 ESVITRDSPRQDCPETLSTPIKLRGTMAKENAQLSEFQEDLVIMAAGLKGDYKNEELIHK 454

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC--GKNNQILQPSDS----- 313
           L ++  V +A+ Y  NA + F  E  KA++ G DE+ +V C    ++ ++ P  S     
Sbjct: 455 LCKETCVADASKYVTNAFEKFLEESRKARDRGCDENDIVYCVDDDDDHVVIPPQSHSEAS 514

Query: 314 ----KPK---SLARKFFACFACH 323
               +PK   S   K F+CF  H
Sbjct: 515 NAAAQPKTQTSFFNKLFSCFIRH 537

[Close]
BLAST of CSPI02G08660 vs. TAIR10
Match: AT3G03520.1 (AT3G03520.1 non-specific phospholipase C3)

HSP 1 Score: 411.8 bits (1057), Expect = 4.0e-115
Identity = 191/311 (61.41%), Postives = 233/311 (74.92%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +N+RKLKY+ NFH + L FKR C+EGKLPNYVVIE RYF + S P NDDHP +DV EGQ 
Sbjct: 215 RNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQN 274

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KE+YEALR+SPQWNEILF++ YDEHGG++DHVP P  GVPNPDG +GP PYNF FDRL
Sbjct: 275 LVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRL 334

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP + +SPWIEPGTV+H P GP+PTS+FEHSSIPAT+KKIF LK FLTKRD+WAGT 
Sbjct: 335 GVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTL 394

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVE---ANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           D V+NR +PRTDCPVTL    + RD++     E   +++FQ EL+Q AAVL+GD  K+IY
Sbjct: 395 DAVINRTSPRTDCPVTLPELPRARDIDIGTQEEDEDLTDFQIELIQAAAVLKGDHIKDIY 454

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKS 313
           P KL +KM V++AA Y E A   F  E +KAKE G DE ++V   K +       S PKS
Sbjct: 455 PFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRDEHEIVDLSKGS----TRHSTPKS 514

Query: 314 LARKFFACFAC 322
             +K F+C  C
Sbjct: 515 FVQKLFSCLIC 521

[Close]
BLAST of CSPI02G08660 vs. TAIR10
Match: AT3G03540.1 (AT3G03540.1 non-specific phospholipase C5)

HSP 1 Score: 397.9 bits (1021), Expect = 6.0e-111
Identity = 188/297 (63.30%), Postives = 228/297 (76.77%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+  FH + L FK+DC+EG LPNYVV+EQR++DL   P NDDHPSHDVSEGQK
Sbjct: 214 RNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVVVEQRWYDLLLNPANDDHPSHDVSEGQK 273

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVP P  GVPNPDG LGPPPYNF F+RL
Sbjct: 274 LVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPLDGVPNPDGILGPPPYNFEFNRL 333

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H   GP   S++EHSSIPATVKKIF LK FLTKRD WAGTF
Sbjct: 334 GVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEHSSIPATVKKIFKLKDFLTKRDSWAGTF 393

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+ R++PR DCP TL+NPVK+R   A E  ++S+FQEELV +AA L+GD K E    K
Sbjct: 394 ESVITRNSPRQDCPETLSNPVKMRGTVAKENAELSDFQEELVIVAAGLKGDYKNEELLYK 453

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC---GKNNQILQPSDSK 308
           L +K  V +A+ Y   A   F  E +KA+E G DE+ +V C     ++ +++P  S+
Sbjct: 454 LCKKTCVSDASKYVTKAFDKFVEESKKARERGGDENDIVFCVDDDDDHNVVKPPPSQ 510

[Close]
BLAST of CSPI02G08660 vs. TAIR10
Match: AT2G26870.1 (AT2G26870.1 non-specific phospholipase C2)

HSP 1 Score: 340.9 bits (873), Expect = 8.7e-94
Identity = 168/282 (59.57%), Postives = 207/282 (73.40%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           ++LRKLKY+  FH +   FK   ++GKLP Y VIEQRY D    P +DDHPSHDV +GQK
Sbjct: 222 QSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLLEPASDDHPSHDVYQGQK 281

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYE LR+SPQWNE L +ITYDEHGG+FDHVP P   VP+PDG +GP P+ F F+RL
Sbjct: 282 FIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPSPDGIVGPDPFLFQFNRL 341

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRG-PDPTSEFEHSSIPATVKKIFGLKQ-FLTKRDQWAG 193
           G+RVPTI VSPWIE GTVVH P G P P+SE+EHSSIPATVKK+F L   FLTKRD+WAG
Sbjct: 342 GIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKKLFNLSSPFLTKRDEWAG 401

Query: 194 TFDIVLN-RHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIY 253
           TF+ +L  R  PRTDCP TL  PVK+R  EANE   ++EFQ+ELVQLAAVL+GD     +
Sbjct: 402 TFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQELVQLAAVLKGDNMLTTF 461

Query: 254 PKKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           PK++ + M+V+E   Y E+A+K F      A   GA++ ++V
Sbjct: 462 PKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELV 503

[Close]
BLAST of CSPI02G08660 vs. TAIR10
Match: AT3G48610.1 (AT3G48610.1 non-specific phospholipase C6)

HSP 1 Score: 339.7 bits (870), Expect = 1.9e-93
Identity = 167/281 (59.43%), Postives = 203/281 (72.24%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLR+LKYI N H +DL FK+D  +GKLP+  VIE RYFDL  LP NDDHPSHDV+ GQK
Sbjct: 228 RNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRYFDLKGLPANDDHPSHDVANGQK 287

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           L+KEVYEALRSSPQWNE L +ITYDEHGGF+DHV  P  G+PNPDG  GP P  F FDRL
Sbjct: 288 LVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPYVGIPNPDGNTGPAPGFFKFDRL 347

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGL-KQFLTKRDQWAGT 193
           GVRVPTI VSPWI+ GTVV   +GP  +SE+EHSSIPAT+KK+F L   FLT RD WA T
Sbjct: 348 GVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPATIKKLFNLSSNFLTHRDAWAAT 407

Query: 194 F-DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYP 253
           F D+V +  TPRTDCP+TL     +R  E  E   +SEFQ E+VQLAAVL GD     +P
Sbjct: 408 FEDVVSHLTTPRTDCPMTLPEVAPMRATEPKEDAALSEFQGEVVQLAAVLNGDHFLSSFP 467

Query: 254 KKLVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVV 293
           +++ +KM+V +A  Y + A   F    ++A + GAD+S +V
Sbjct: 468 EEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADKSAIV 508

[Close]
BLAST of CSPI02G08660 vs. NCBI nr
Match: gi|449442455|ref|XP_004138997.1| (PREDICTED: non-specific phospholipase C3-like [Cucumis sativus])

HSP 1 Score: 640.2 bits (1650), Expect = 2.0e-180
Identity = 305/310 (98.39%), Postives = 308/310 (99.35%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK
Sbjct: 220 RNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 279

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
           LIKEVYEALRS PQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL
Sbjct: 280 LIKEVYEALRSCPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 339

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           G+RVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF
Sbjct: 340 GLRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 399

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           DIVLNRHTPRTDCPVTLNNPVKLRDVEANE RQISEFQEELVQLAAVLRGDGKKEIYPKK
Sbjct: 400 DIVLNRHTPRTDCPVTLNNPVKLRDVEANEMRQISEFQEELVQLAAVLRGDGKKEIYPKK 459

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSLAR
Sbjct: 460 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSLAR 519

Query: 314 KFFACFACHG 324
           KFFACFACHG
Sbjct: 520 KFFACFACHG 529

[Close]
BLAST of CSPI02G08660 vs. NCBI nr
Match: gi|700206297|gb|KGN61416.1| (hypothetical protein Csa_2G119380 [Cucumis sativus])

HSP 1 Score: 639.4 bits (1648), Expect = 3.3e-180
Identity = 305/309 (98.71%), Postives = 307/309 (99.35%), Query Frame = 1 

Query: 15  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 74
           NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL
Sbjct: 61  NLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQKL 120

Query: 75  IKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 134
           IKEVYEALRS PQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG
Sbjct: 121 IKEVYEALRSCPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRLG 180

Query: 135 VRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 194
           +RVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD
Sbjct: 181 LRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTFD 240

Query: 195 IVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKKL 254
           IVLNRHTPRTDCPVTLNNPVKLRDVEANE RQISEFQEELVQLAAVLRGDGKKEIYPKKL
Sbjct: 241 IVLNRHTPRTDCPVTLNNPVKLRDVEANEMRQISEFQEELVQLAAVLRGDGKKEIYPKKL 300

Query: 255 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLARK 314
           VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSLARK
Sbjct: 301 VEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSLARK 360

Query: 315 FFACFACHG 324
           FFACFACHG
Sbjct: 361 FFACFACHG 369

[Close]
BLAST of CSPI02G08660 vs. NCBI nr
Match: gi|659114666|ref|XP_008457171.1| (PREDICTED: non-specific phospholipase C3-like [Cucumis melo])

HSP 1 Score: 594.3 bits (1531), Expect = 1.2e-166
Identity = 285/298 (95.64%), Postives = 291/298 (97.65%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYI NFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK
Sbjct: 212 RNLRKLKYIMNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 271

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            IKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPD RLGPPPYNFNFDRL
Sbjct: 272 FIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDARLGPPPYNFNFDRL 331

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPTIFVSPWIEPGTVVHRP GPDPTSEFEHSSI ATVKKIFGLK+FLTKRDQWAGTF
Sbjct: 332 GVRVPTIFVSPWIEPGTVVHRPTGPDPTSEFEHSSIAATVKKIFGLKEFLTKRDQWAGTF 391

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           DIVLNRHTPRTDCPVTLNNPVKLR+VEAN+ RQISEFQEELVQLAAVL+GD KKEIYPKK
Sbjct: 392 DIVLNRHTPRTDCPVTLNNPVKLRNVEANDRRQISEFQEELVQLAAVLKGDVKKEIYPKK 451

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSL 312
           LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPS+SKPKSL
Sbjct: 452 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSNSKPKSL 509

[Close]
BLAST of CSPI02G08660 vs. NCBI nr
Match: gi|720054739|ref|XP_010273152.1| (PREDICTED: non-specific phospholipase C4-like isoform X2 [Nelumbo nucifera])

HSP 1 Score: 456.1 bits (1172), Expect = 5.3e-125
Identity = 218/315 (69.21%), Postives = 255/315 (80.95%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKYIKNFH FD++FK  C+EGKLPNYVV+EQRYFDL  LPGNDDHPSHDVSEGQK
Sbjct: 210 RNLRKLKYIKNFHQFDMNFKSHCKEGKLPNYVVVEQRYFDLKLLPGNDDHPSHDVSEGQK 269

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALR+SPQWNEILF+ITYDEHGGF+DHVP P+ GVP+PD    P P+ F FDRL
Sbjct: 270 FVKEVYEALRASPQWNEILFIITYDEHGGFYDHVPTPT-GVPSPDDIASPGPFYFKFDRL 329

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVP IF+SPWIE GTV+HRP GP P+SEFEHSSIPATVKKIF LK+FLTKRD WAGTF
Sbjct: 330 GVRVPAIFISPWIERGTVLHRPSGPYPSSEFEHSSIPATVKKIFNLKEFLTKRDAWAGTF 389

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + VL R +PRTDCPVTL  P KLR  +ANE  ++SEFQ ELVQLAA L+GD +K+IYP K
Sbjct: 390 ETVLTRESPRTDCPVTLPQPRKLRSGDANEGAKLSEFQVELVQLAAALKGDHRKDIYPNK 449

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVC-----GKNN-QILQPSDSK 313
           LVEKM+V +A SYC++A K F   CEKAK+NGADES+++V       KN+  +++    K
Sbjct: 450 LVEKMTVKDAVSYCKDAFKKFLEGCEKAKKNGADESEIIVLPSLPPSKNSTPVVEQPPPK 509

Query: 314 PKSLARKFFACFACH 323
            KS   K FACFAC+
Sbjct: 510 SKSFVHKMFACFACN 523

[Close]
BLAST of CSPI02G08660 vs. NCBI nr
Match: gi|590639930|ref|XP_007029811.1| (Non-specific phospholipase C4 [Theobroma cacao])

HSP 1 Score: 454.1 bits (1167), Expect = 2.0e-124
Identity = 212/306 (69.28%), Postives = 244/306 (79.74%), Query Frame = 1 

Query: 14  KNLRKLKYIKNFHIFDLDFKRDCREGKLPNYVVIEQRYFDLASLPGNDDHPSHDVSEGQK 73
           +NLRKLKY+KNFH FDL FK  C EGKLPNYVV+EQRYFDL S+P NDDHPSHDVSEGQK
Sbjct: 212 RNLRKLKYLKNFHQFDLHFKEHCEEGKLPNYVVVEQRYFDLLSVPANDDHPSHDVSEGQK 271

Query: 74  LIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPPPSAGVPNPDGRLGPPPYNFNFDRL 133
            +KEVYEALR+SPQWNE+L +I YDEHGGF+DHVP P+ GVP+PD  +GP PY F FDRL
Sbjct: 272 FVKEVYEALRASPQWNEMLLVIAYDEHGGFYDHVPTPTIGVPSPDDIIGPEPYYFKFDRL 331

Query: 134 GVRVPTIFVSPWIEPGTVVHRPRGPDPTSEFEHSSIPATVKKIFGLKQFLTKRDQWAGTF 193
           GVRVPT F+SPWIEPGTV+H P GP PTSEFEHSSIPATVK IF LK+FLTKRD WAGTF
Sbjct: 332 GVRVPTFFISPWIEPGTVIHGPSGPYPTSEFEHSSIPATVKTIFNLKEFLTKRDAWAGTF 391

Query: 194 DIVLNRHTPRTDCPVTLNNPVKLRDVEANETRQISEFQEELVQLAAVLRGDGKKEIYPKK 253
           + V+NR  PRTDCPVTL  PVK+RD  A ET ++SEFQ+ELVQ+AAVL GD KK+IYP K
Sbjct: 392 ECVINRENPRTDCPVTLTEPVKMRDAAAKETAKLSEFQQELVQMAAVLSGDHKKKIYPHK 451

Query: 254 LVEKMSVVEAASYCENALKSFFNECEKAKENGADESQVVVCGKNNQILQPSDSKPKSLAR 313
           LVE M+V EA  Y + A K F +EC+KAKE G DES++V  G+      P     KS+ R
Sbjct: 452 LVENMTVAEAVKYVDGAFKKFCHECQKAKERGIDESEIVDLGE-----LPQRLNSKSVVR 511

Query: 314 KFFACF 320
           K F+CF
Sbjct: 512 KMFSCF 512

The following BLAST results are available for this feature:
BLAST of CSPI02G08660 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NPC4_ARATH5.4e-11460.99Non-specific phospholipase C4 OS=Arabidopsis thaliana GN=NPC4 PE=1 SV=1[more]
NPC3_ARATH7.1e-11461.41Non-specific phospholipase C3 OS=Arabidopsis thaliana GN=NPC3 PE=2 SV=1[more]
NPC5_ARATH1.1e-10963.30Non-specific phospholipase C5 OS=Arabidopsis thaliana GN=NPC5 PE=2 SV=1[more]
NPC2_ARATH1.5e-9259.57Non-specific phospholipase C2 OS=Arabidopsis thaliana GN=NPC2 PE=2 SV=1[more]
NPC6_ARATH3.4e-9259.43Non-specific phospholipase C6 OS=Arabidopsis thaliana GN=NPC6 PE=2 SV=1[more]
BLAST of CSPI02G08660 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LN71_CUCSA2.3e-18098.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G119380 PE=4 SV=1[more]
A0A061F088_THECC1.4e-12469.28Non-specific phospholipase C4 OS=Theobroma cacao GN=TCM_025686 PE=4 SV=1[more]
M5X2R5_PRUPE3.8e-12268.51Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004364mg PE=4 SV=1[more]
A0A0K9QM76_SPIOL1.4e-12167.31Uncharacterized protein OS=Spinacia oleracea GN=SOVF_169900 PE=4 SV=1[more]
V4TBG2_9ROSI2.5e-12168.39Uncharacterized protein OS=Citrus clementina GN=CICLE_v10031213mg PE=4 SV=1[more]
BLAST of CSPI02G08660 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G03530.13.1e-11560.99 non-specific phospholipase C4[more]
AT3G03520.14.0e-11561.41 non-specific phospholipase C3[more]
AT3G03540.16.0e-11163.30 non-specific phospholipase C5[more]
AT2G26870.18.7e-9459.57 non-specific phospholipase C2[more]
AT3G48610.11.9e-9359.43 non-specific phospholipase C6[more]
BLAST of CSPI02G08660 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442455|ref|XP_004138997.1|2.0e-18098.39PREDICTED: non-specific phospholipase C3-like [Cucumis sativus][more]
gi|700206297|gb|KGN61416.1|3.3e-18098.71hypothetical protein Csa_2G119380 [Cucumis sativus][more]
gi|659114666|ref|XP_008457171.1|1.2e-16695.64PREDICTED: non-specific phospholipase C3-like [Cucumis melo][more]
gi|720054739|ref|XP_010273152.1|5.3e-12569.21PREDICTED: non-specific phospholipase C4-like isoform X2 [Nelumbo nucifera][more]
gi|590639930|ref|XP_007029811.1|2.0e-12469.28Non-specific phospholipase C4 [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR007312Phosphoesterase
IPR017850Alkaline_phosphatase_core_sf
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G08660.1CSPI02G08660.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007312PhosphoesterasePFAMPF04185Phosphoesterasecoord: 13..180
score: 1.7
IPR017850Alkaline-phosphatase-like, core domainunknownSSF53649Alkaline phosphatase-likecoord: 74..181
score: 1.3
NoneNo IPR availablePANTHERPTHR31956FAMILY NOT NAMEDcoord: 15..310
score: 5.1E
NoneNo IPR availablePANTHERPTHR31956:SF1NON-SPECIFIC PHOSPHOLIPASE C3-RELATEDcoord: 15..310
score: 5.1E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G08660Csa2G119380Cucumber (Chinese Long) v2cpicuB065
CSPI02G08660MELO3C020373Melon (DHL92) v3.5.1cpimeB133
CSPI02G08660Cucsa.265920Cucumber (Gy14) v1cgycpiB403
CSPI02G08660CmaCh08G010090Cucurbita maxima (Rimu)cmacpiB901
CSPI02G08660CsGy2G008920Cucumber (Gy14) v2cgybcpiB056
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G08660Watermelon (97103) v2cpiwmbB150
CSPI02G08660Wax gourdcpiwgoB123
CSPI02G08660Wild cucumber (PI 183967)cpicpiB063
CSPI02G08660Cucurbita maxima (Rimu)cmacpiB355
CSPI02G08660Cucurbita moschata (Rifu)cmocpiB347
CSPI02G08660Cucurbita moschata (Rifu)cmocpiB813
CSPI02G08660Cucurbita moschata (Rifu)cmocpiB881
CSPI02G08660Cucumber (Chinese Long) v2cpicuB072
CSPI02G08660Melon (DHL92) v3.5.1cpimeB145
CSPI02G08660Watermelon (Charleston Gray)cpiwcgB157
CSPI02G08660Watermelon (97103) v1cpiwmB128
CSPI02G08660Watermelon (97103) v1cpiwmB175
CSPI02G08660Cucurbita pepo (Zucchini)cpecpiB127
CSPI02G08660Cucurbita pepo (Zucchini)cpecpiB305
CSPI02G08660Bottle gourd (USVL1VR-Ls)cpilsiB086
CSPI02G08660Bottle gourd (USVL1VR-Ls)cpilsiB128
CSPI02G08660Melon (DHL92) v3.6.1cpimedB126
CSPI02G08660Melon (DHL92) v3.6.1cpimedB139
CSPI02G08660Silver-seed gourdcarcpiB0260
CSPI02G08660Silver-seed gourdcarcpiB0996
CSPI02G08660Silver-seed gourdcarcpiB1110
CSPI02G08660Cucumber (Chinese Long) v3cpicucB080
CSPI02G08660Cucumber (Chinese Long) v3cpicucB093