MELO3C026739 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C026739
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Phospholipid N-methyltransferase
Location	chr4 : 24988032 .. 24988397 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAGAGCTACTCCTCCTCTTCTTCCTCCTGGAAATCATTTTCCACAGAAAGTGGAGCGAAAATCCGGCGAGAAAATTATCAGAATGGGCATTTTGGCCGTAATTGGAGTATTGTTGCCATTTCCGTACTACTACGTCTTATGGAATCGTCCACAGTTGTGGGTGAACTTGTGCGGCAAATCGCGCGAACCCTGCAAGTTGATGGCCCAGGTCTCGGTTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCATTCATGTCTCCTCCTTCTCATGGCCCCCGCCTCTATACTTCTGGCCTCTCATGGCTTTCGGACAGTTCTTGAACTTCAGGAATCTCTCTCTCTCTCTCTCTCTGATGTGCTGA

mRNA sequence

ATGAGAAGAGCTACTCCTCCTCTTCTTCCTCCTGGAAATCATTTTCCACAGAAAGTGGAGCGAAAATCCGGCGAGAAAATTATCAGAATGGGCATTTTGGCCGTAATTGGAGTATTGTTGCCATTTCCGTACTACTACGTCTTATGGAATCGTCCACAGTTGTGGGTGAACTTGTGCGGCAAATCGCGCGAACCCTGCAAGTTGATGGCCCAGGTCTCGGTTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCATTCATGTCTCCTCCTTCTCATGGCCCCCGCCTCTATACTTCTGGCCTCTCATGGCTTTCGGACAGTTCTTGAACTTCAGGAATCTCTCTCTCTCTCTCTCTCTGATGTGCTGA

Coding sequence (CDS)

ATGAGAAGAGCTACTCCTCCTCTTCTTCCTCCTGGAAATCATTTTCCACAGAAAGTGGAGCGAAAATCCGGCGAGAAAATTATCAGAATGGGCATTTTGGCCGTAATTGGAGTATTGTTGCCATTTCCGTACTACTACGTCTTATGGAATCGTCCACAGTTGTGGGTGAACTTGTGCGGCAAATCGCGCGAACCCTGCAAGTTGATGGCCCAGGTCTCGGTTTTTCTCAAGTTGCTTCAGTTCCTCTCCATCATTCATGTCTCCTCCTTCTCATGGCCCCCGCCTCTATACTTCTGGCCTCTCATGGCTTTCGGACAGTTCTTGAACTTCAGGAATCTCTCTCTCTCTCTCTCTCTGATGTGCTGA

Protein sequence

MRRATPPLLPPGNHFPQKVERKSGEKIIRMGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRNLSLSLSLMC*

Homology

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BLAST of MELO3C026739 vs. Swiss-Prot
Match: PLMT_ARATH (Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana GN=PLMT PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.2e-28
Identity = 56/82 (68.29%), Postives = 63/82 (76.83%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MG+LA IGVLLPFP+Y+ LW   Q WVNLCG+ R+P  +MA+VS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
            SWPPPLYFWPLMAFGQFLNFR
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFR 82

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BLAST of MELO3C026739 vs. TrEMBL
Match: A0A0A0LCJ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710860 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 2.1e-41
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGI AVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPLMAFGQFLNFR
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. TrEMBL
Match: A0A0D2R7K2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G136500 PE=4 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 5.8e-31
Identity = 63/82 (76.83%), Postives = 71/82 (86.59%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGILA IGVLLPFP+YY+LW  PQ WVNLCGK R+P K+MA VS F+KL+QF+S+  VSS
Sbjct: 1   MGILAGIGVLLPFPFYYLLWTYPQSWVNLCGKGRDPSKVMALVSHFIKLIQFVSLFSVSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPL AFGQFLNFR
Sbjct: 61  FSWPPPLYFWPLFAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. TrEMBL
Match: B9RGL2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1441570 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 4.9e-30
Identity = 61/82 (74.39%), Postives = 67/82 (81.71%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MG+LA +GVLLPFPYYY LW  PQ WVNLCGK  +PCK+MA VS FLKLLQF+S+  VSS
Sbjct: 1   MGVLAAVGVLLPFPYYYWLWTNPQSWVNLCGKGSDPCKVMAYVSHFLKLLQFISLFSVSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSW PPLYFWPL  FGQFLN R
Sbjct: 61  FSWLPPLYFWPLFGFGQFLNVR 82

[Close]

BLAST of MELO3C026739 vs. TrEMBL
Match: A0A103XYB1_CYNCS (Phosphatidylethanolamine N-methyltransferase/Methylene-fatty-acyl-phospholipid synthase OS=Cynara cardunculus var. scolymus GN=Ccrd_022720 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 4.9e-30
Identity = 60/82 (73.17%), Postives = 67/82 (81.71%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGILA  GV+ PFP+YY LWN PQ WVNLCGK R+PCK+MA VS FLKL+QFLS+  VS+
Sbjct: 1   MGILACFGVVTPFPFYYWLWNHPQTWVNLCGKGRDPCKIMAMVSHFLKLVQFLSLFSVST 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPL   GQFLN R
Sbjct: 61  FSWPPPLYFWPLFLLGQFLNLR 82

[Close]

BLAST of MELO3C026739 vs. TrEMBL
Match: A0A0B0MVI6_GOSAR (Putative methylene-fatty-acyl-phospholipid synthase OS=Gossypium arboreum GN=F383_32328 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 8.3e-30
Identity = 61/82 (74.39%), Postives = 70/82 (85.37%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGI+A IGVLLPFP+YY+LW  PQ WVNLCGK R+P K+MA VS F+KL+QF+S+   SS
Sbjct: 1   MGIVAGIGVLLPFPFYYLLWTYPQSWVNLCGKGRDPSKVMALVSHFIKLIQFVSLFSGSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPL AFGQFLNFR
Sbjct: 61  FSWPPPLYFWPLFAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. TAIR10
Match: AT1G80860.2 (AT1G80860.2 phospholipid N-methyltransferase)

HSP 1 Score: 126.3 bits (316), Expect = 1.3e-29
Identity = 56/82 (68.29%), Postives = 63/82 (76.83%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MG+LA IGVLLPFP+Y+ LW   Q WVNLCG+ R+P  +MA+VS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
            SWPPPLYFWPLMAFGQFLNFR
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. NCBI nr
Match: gi|659130401|ref|XP_008465151.1| (PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo])

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-59
Identity = 111/111 (100.00%), Postives = 111/111 (100.00%), Query Frame = 1


Query: 1   MRRATPPLLPPGNHFPQKVERKSGEKIIRMGILAVIGVLLPFPYYYVLWNRPQLWVNLCG 60
           MRRATPPLLPPGNHFPQKVERKSGEKIIRMGILAVIGVLLPFPYYYVLWNRPQLWVNLCG
Sbjct: 1   MRRATPPLLPPGNHFPQKVERKSGEKIIRMGILAVIGVLLPFPYYYVLWNRPQLWVNLCG 60

Query: 61  KSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFR 112
           KSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFR
Sbjct: 61  KSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFR 111

[Close]

BLAST of MELO3C026739 vs. NCBI nr
Match: gi|449439767|ref|XP_004137657.1| (PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus])

HSP 1 Score: 176.4 bits (446), Expect = 3.0e-41
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGI AVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPLMAFGQFLNFR
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. NCBI nr
Match: gi|700203526|gb|KGN58659.1| (hypothetical protein Csa_3G710860 [Cucumis sativus])

HSP 1 Score: 176.4 bits (446), Expect = 3.0e-41
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGI AVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPLMAFGQFLNFR
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. NCBI nr
Match: gi|823238474|ref|XP_012451890.1| (PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Gossypium raimondii])

HSP 1 Score: 141.7 bits (356), Expect = 8.3e-31
Identity = 63/82 (76.83%), Postives = 71/82 (86.59%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGILA IGVLLPFP+YY+LW  PQ WVNLCGK R+P K+MA VS F+KL+QF+S+  VSS
Sbjct: 1   MGILAGIGVLLPFPFYYLLWTYPQSWVNLCGKGRDPSKVMALVSHFIKLIQFVSLFSVSS 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPL AFGQFLNFR
Sbjct: 61  FSWPPPLYFWPLFAFGQFLNFR 82

[Close]

BLAST of MELO3C026739 vs. NCBI nr
Match: gi|976912568|gb|KVH99046.1| (Phosphatidylethanolamine N-methyltransferase/Methylene-fatty-acyl-phospholipid synthase [Cynara cardunculus var. scolymus])

HSP 1 Score: 138.7 bits (348), Expect = 7.0e-30
Identity = 60/82 (73.17%), Postives = 67/82 (81.71%), Query Frame = 1


Query: 30  MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 89
           MGILA  GV+ PFP+YY LWN PQ WVNLCGK R+PCK+MA VS FLKL+QFLS+  VS+
Sbjct: 1   MGILACFGVVTPFPFYYWLWNHPQTWVNLCGKGRDPCKIMAMVSHFLKLVQFLSLFSVST 60

Query: 90  FSWPPPLYFWPLMAFGQFLNFR 112
           FSWPPPLYFWPL   GQFLN R
Sbjct: 61  FSWPPPLYFWPLFLLGQFLNLR 82

The following BLAST results are available for this feature:

BLAST of MELO3C026739 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLMT_ARATH	2.2e-28	68.29	Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana GN...	[more]

BLAST of MELO3C026739 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCJ2_CUCSA	2.1e-41	98.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710860 PE=4 SV=1	[more]
A0A0D2R7K2_GOSRA	5.8e-31	76.83	Uncharacterized protein OS=Gossypium raimondii GN=B456_010G136500 PE=4 SV=1	[more]
B9RGL2_RICCO	4.9e-30	74.39	Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1441570 PE=4 SV=1	[more]
A0A103XYB1_CYNCS	4.9e-30	73.17	Phosphatidylethanolamine N-methyltransferase/Methylene-fatty-acyl-phospholipid s...	[more]
A0A0B0MVI6_GOSAR	8.3e-30	74.39	Putative methylene-fatty-acyl-phospholipid synthase OS=Gossypium arboreum GN=F38...	[more]

BLAST of MELO3C026739 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G80860.2	1.3e-29	68.29	phospholipid N-methyltransferase	[more]

BLAST of MELO3C026739 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659130401\|ref\|XP_008465151.1\|	1.1e-59	100.00	PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo]	[more]
gi\|449439767\|ref\|XP_004137657.1\|	3.0e-41	98.78	PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativu...	[more]
gi\|700203526\|gb\|KGN58659.1\|	3.0e-41	98.78	hypothetical protein Csa_3G710860 [Cucumis sativus]	[more]
gi\|823238474\|ref\|XP_012451890.1\|	8.3e-31	76.83	PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Gossypium raim...	[more]
gi\|976912568\|gb\|KVH99046.1\|	7.0e-30	73.17	Phosphatidylethanolamine N-methyltransferase/Methylene-fatty-acyl-phospholipid s...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR024960	PEMT/MFAP

Vocabulary: **Biological Process**
Term	Definition
GO:0006644	phospholipid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008170	N-methyltransferase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006544	glycine metabolic process
biological_process	GO:0006563	L-serine metabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0008654	phospholipid biosynthetic process
biological_process	GO:0006566	threonine metabolic process
biological_process	GO:0006644	phospholipid metabolic process
biological_process	GO:0006656	phosphatidylcholine biosynthetic process
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0080101	phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
molecular_function	GO:0000773	phosphatidyl-N-methylethanolamine N-methyltransferase activity
molecular_function	GO:0008170	N-methyltransferase activity
molecular_function	GO:0004608	phosphatidylethanolamine N-methyltransferase activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
MU55375	melon EST collection version 4.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C026739T1	MELO3C026739T1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
MU55375	MU55375	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024960	Phosphatidyl-N-methylethanolamine/N-methyltransferase	PANTHER	PTHR15458	FAMILY NOT NAMED	coord: 30..111 score: 2.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C026739	Csa3G710860	Cucumber (Chinese Long) v2	cumeB164
MELO3C026739	ClCG10G012930	Watermelon (Charleston Gray)	mewcgB315
MELO3C026739	Cla97C10G195790	Watermelon (97103) v2	mewmbB316
MELO3C026739	Bhi11G001251	Wax gourd	mewgoB374
MELO3C026739	CSPI03G30340	Wild cucumber (PI 183967)	cpimeB199
MELO3C026739	Cucsa.177240	Cucumber (Gy14) v1	cgymeB317
MELO3C026739	CmaCh01G001860	Cucurbita maxima (Rimu)	cmameB426
MELO3C026739	CmoCh01G001930	Cucurbita moschata (Rifu)	cmomeB416
MELO3C026739	Cp4.1LG02g15250	Cucurbita pepo (Zucchini)	cpemeB517
MELO3C026739	CsGy3G028140	Cucumber (Gy14) v2	cgybmeB172
MELO3C026739	Carg04194	Silver-seed gourd	carmeB0206
MELO3C026739	CsaV3_3G032790	Cucumber (Chinese Long) v3	cucmeB205

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C026739	Cucumber (Gy14) v1	cgymeB316
MELO3C026739	Cucurbita maxima (Rimu)	cmameB226
MELO3C026739	Cucurbita moschata (Rifu)	cmomeB210
MELO3C026739	Cucurbita moschata (Rifu)	cmomeB326
MELO3C026739	Watermelon (97103) v1	mewmB346
MELO3C026739	Cucurbita pepo (Zucchini)	cpemeB133
MELO3C026739	Cucurbita pepo (Zucchini)	cpemeB566
MELO3C026739	Bottle gourd (USVL1VR-Ls)	lsimeB260
MELO3C026739	Silver-seed gourd	carmeB0360
MELO3C026739	Silver-seed gourd	carmeB0811