MELO3C025263 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C025263 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.5e-26
Identity = 55/116 (47.41%), Postives = 71/116 (61.21%), Query Frame = 1

Query: 327 GDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDF 386
           GDY    G E L++Q+AE   +R G PPA+ S ++ LP + I+K +L  +   CA+C D 
Sbjct: 171 GDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDD 230

Query: 387 LLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTA 443
              G E  Q+PC HLYH  C+LPWL   NSCP+CR+ELPTDD DYE   R +  T+
Sbjct: 231 FEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTS 286

BLAST of MELO3C025263 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.9e-26
Identity = 53/115 (46.09%), Postives = 73/115 (63.48%), Query Frame = 1

Query: 324 GDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 383
           G+ GDY    G E L++Q+AE   +R G PPA+ S +  LP + ++K+ LK +   CA+C
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 384 KDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSS 439
            D    G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD DYE   + S
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGS 274

BLAST of MELO3C025263 vs. Swiss-Prot
Match: CIP8_ARATH (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana GN=CIP8 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.1e-24
Identity = 70/210 (33.33%), Postives = 102/210 (48.57%), Query Frame = 1

Query: 242 DEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPGH 301
           +EED+    T  + E E      R +   +   S + R    +W E L   E + S    
Sbjct: 131 EEEDEEENLTVNDEEDEEDDLRRRNRFPLTTTQSRTGRNRILDWAEILMGIE-DNSIEFR 190

Query: 302 IRRNRRFYSITNYAQSELLSYVGDSGDYLD-RQGFEDLLEQIAETTSS----RRGAPPAA 361
           +  +R               Y G+  DY+D   G+E LL+ +AE        RRGAPPAA
Sbjct: 191 MESDR---------------YAGNPADYIDDAAGYEALLQNLAEGDGGGGGGRRGAPPAA 250

Query: 362 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 421
            S ++ L    +S    +   + CA+CKD +++G    +LPC H YH  CI+PWL  RNS
Sbjct: 251 KSAIEALETFEVSSSEGEMVMV-CAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLGTRNS 310

Query: 422 CPLCRYELPTDDRDYEEVKRSSINTAATHA 447
           CP+CR++L TDD +YEE ++   +T +  A
Sbjct: 311 CPVCRFQLETDDAEYEEERKKRTSTVSDSA 323

BLAST of MELO3C025263 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.7e-22
Identity = 64/173 (36.99%), Postives = 89/173 (51.45%), Query Frame = 1

Query: 324 GDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 383
           G++GDY    G E+L EQ++  T+ RRG PPA  S +  LP + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTT-RRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 384 KDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAA 443
           KD   +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP+         R    T  
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNR----TTP 254

Query: 444 THALQPWVVGLENPSGLNGVGANSEFESSQEEDYRNIDSAVISSNNSGRGGRR 497
           T   +       + S  NG    + F S     +R+  S+  SS+   RGG R
Sbjct: 255 TRNYRSSSSSSSSNSRENGNERRNPFSSFW--PFRSSGSS--SSSTQNRGGTR 298

BLAST of MELO3C025263 vs. Swiss-Prot
Match: RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 8.4e-22
Identity = 59/155 (38.06%), Postives = 88/155 (56.77%), Query Frame = 1

Query: 280 GNSSNWTEQLSSPEYEGSTPGHIRRNRRFYSI--TNYAQSELLSYVGDSGDYLDRQGFED 339
           G      E++ + E +G+T     R RR Y     + + S L        + L   GFE 
Sbjct: 114 GGGGGAGERVENEEGDGAT-----RERRAYEFYYDDGSGSGLRPLPDSVSEILMGSGFER 173

Query: 340 LLEQIAETTSS-----RRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVE 399
           LLEQ+++  +S     R G PPA+ S +++LPR+ IS  H K ++ +CA+C +    G+E
Sbjct: 174 LLEQLSQIEASGNGIGRSGNPPASKSAIESLPRVEISDCHTKAEA-NCAVCTEVFEAGIE 233

Query: 400 VNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTD 428
             ++PC H++H  CI+PWLS RNSCP+CR+ELP+D
Sbjct: 234 GREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSD 262

BLAST of MELO3C025263 vs. TrEMBL
Match: A0A0A0L2Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G337330 PE=4 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 6.0e-309
Identity = 524/560 (93.57%), Postives = 539/560 (96.25%), Query Frame = 1

Query: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60
           MDIDLQRILEEFPQS VHVIMN+NQRVDEHSPIQLPTCAFCQRVL  DSDA SEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR MRSSSESPENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA+DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDVVSNRTYGGDSDASLD-GHSFLDTDIFILPGEGSDTDTDIDPMRAGIGN 240
           GPYRHSYGDSDVVSNRTYGG+SD SLD GHSFLDTD+FILPGEGSDTDTDIDPMRAG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240

Query: 241 WDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQE+GSSEARPQLQN +VSSASRRGNSSNW EQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAET-TSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFE+LLEQIAET TSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHP+CILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480
           CPLCRYELPTDDRDYEEVK+SSIN AA H LQP VVGLENPSGLNGVGAN EFE+SQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480

Query: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540
            RNIDSAVISSNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GGQLTDGRQ
Sbjct: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540

Query: 541 LDARSAESSNQRENRTRRWW 557
           LDA SAESSNQRENR+RRWW
Sbjct: 541 LDAHSAESSNQRENRSRRWW 560

BLAST of MELO3C025263 vs. TrEMBL
Match: A5BWM7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040928 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 7.9e-144
Identity = 284/572 (49.65%), Postives = 372/572 (65.03%), Query Frame = 1

Query: 4   DLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDICGD 63
           D + + +E  QS V    NS+  ++ HS +    CA CQ++L  D + T E EPI +CGD
Sbjct: 81  DSRSVPQELSQSTVPNSSNSDSLLN-HSDLPA-ACALCQKILLPDDEGTGELEPISMCGD 140

Query: 64  CKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISSH 123
           CKFL LED+ +P +DS+ R  PR RRT  SSSES ENLFSQ F+HMINLVRQ QS++S H
Sbjct: 141 CKFLFLEDLRSPAQDSHWRRPPRVRRTRYSSSESIENLFSQHFSHMINLVRQNQSTVSGH 200

Query: 124 GDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGPY 183
            D+  D       +Q  SS TTP+ SRRWR+V SD+E+DG D+ DS++GESESN S+G Y
Sbjct: 201 EDQLVDVDPGARLLQRPSSRTTPSGSRRWRRVPSDTESDGFDNLDSLYGESESNISYGGY 260

Query: 184 RHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIGNWD 243
           R   G+SD +S   YGGDSDAS+DGHSFLDT++++ P +GS  ++DTDIDPM A +  W+
Sbjct: 261 RLFNGESDAISFSAYGGDSDASVDGHSFLDTEMYVQPDDGSNLNSDTDIDPMHARLSQWN 320

Query: 244 SSDEEDDHGEFTEAETEQE--------IGSSEARPQLQNSNVSSASRRGNSSNWTEQLSS 303
           S D+++D  E  E + E E        + S+EA    +N   SS++      N   ++ S
Sbjct: 321 SDDQDEDDDEEXEEDDEWEEADPEENVVESAEAEVHPRNFFPSSSNGNNVLVNMRRRIHS 380

Query: 304 PEYEGSTPGHIRRNRRFY---SITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSS 363
           PE+ G+ P  +R  R+ +     +N  +SE+L Y G+S DYLD +GFE+ LE +AET SS
Sbjct: 381 PEFLGAIPWRLREGRQTFISNFFSNLDESEVLPYFGNSEDYLDARGFEEFLEHLAETDSS 440

Query: 364 RRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCIL 423
           RRGAPPAAVSFV +LPR+VI++EH K D + CAICKD L VG EVNQLPC HLYHP CIL
Sbjct: 441 RRGAPPAAVSFVNSLPRVVINEEHEKRDGLVCAICKDVLSVGTEVNQLPCFHLYHPYCIL 500

Query: 424 PWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSE 483
           PWL+ARNSCPLCRYELPTDD+DYEE K +    A+ H +    +  ++ S     GA ++
Sbjct: 501 PWLTARNSCPLCRYELPTDDKDYEEGKLN----ASIHEIPQQDLSDDSSSDTTD-GAEAD 560

Query: 484 FESSQEEDYRNIDSAVISSNNSGR-GGRRRWLFV-AAPIVGLVGIALMLWFGNPLCDQRV 543
                  + R +   V   N S R GGR RW F+ AAPI GLVG+AL+LW   P+ D+R 
Sbjct: 561 DTCEISPECRELADEVPVRNGSDREGGRGRWFFLAAAPIAGLVGMALVLWLRIPVTDRRG 620

Query: 544 PGGQLTDGRQLDARSAESS--NQRENRTRRWW 557
             G+     Q    S+ SS  NQRENR+RRWW
Sbjct: 621 AIGRSNIQGQTQLHSSGSSLPNQRENRSRRWW 645

BLAST of MELO3C025263 vs. TrEMBL
Match: F6HMS0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0056g00320 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 7.9e-144
Identity = 284/572 (49.65%), Postives = 372/572 (65.03%), Query Frame = 1

Query: 4   DLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDICGD 63
           D + + +E  QS V    NS+  ++ HS +    CA CQ++L  D + T E EPI +CGD
Sbjct: 23  DSRSVPQELSQSTVPNSSNSDSLLN-HSDLPA-ACALCQKILLPDDEGTGELEPISMCGD 82

Query: 64  CKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISSH 123
           CKFL LED+ +P +DS+ R  PR RRT  SSSES ENLFSQ F+HMINLVRQ QS++S H
Sbjct: 83  CKFLFLEDLRSPAQDSHWRRPPRVRRTRYSSSESIENLFSQHFSHMINLVRQNQSTVSGH 142

Query: 124 GDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGPY 183
            D+  D       +Q  SS TTP+ SRRWR+V SD+E+DG D+ DS++GESESN S+G Y
Sbjct: 143 EDQLVDVDPGARLLQRPSSRTTPSGSRRWRRVPSDTESDGFDNLDSLYGESESNISYGGY 202

Query: 184 RHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIGNWD 243
           R   G+SD +S   YGGDSDAS+DGHSFLDT++++ P +GS  ++DTDIDPM A +  W+
Sbjct: 203 RLFNGESDAISFSAYGGDSDASVDGHSFLDTEMYVQPDDGSNLNSDTDIDPMHARLSQWN 262

Query: 244 SSDEEDDHGEFTEAETEQE--------IGSSEARPQLQNSNVSSASRRGNSSNWTEQLSS 303
           S D+++D  E  E + E E        + S+EA    +N   SS++      N   ++ S
Sbjct: 263 SDDQDEDDDEEEEEDDEWEEADPEENVVESAEAEVHPRNFFPSSSNGNNVLVNMRRRIHS 322

Query: 304 PEYEGSTPGHIRRNRRFY---SITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSS 363
           PE+ G+ P  +R  R+ +     +N  +SE+L Y G+S DYLD +GFE+ LE +AET SS
Sbjct: 323 PEFLGAIPWRLREGRQTFISNFFSNLDESEVLPYFGNSEDYLDARGFEEFLEHLAETDSS 382

Query: 364 RRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCIL 423
           RRGAPPAAVSFV +LPR+VI++EH K D + CAICKD L VG EVNQLPC HLYHP CIL
Sbjct: 383 RRGAPPAAVSFVNSLPRVVINEEHEKRDGLVCAICKDVLSVGTEVNQLPCFHLYHPYCIL 442

Query: 424 PWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSE 483
           PWL+ARNSCPLCRYELPTDD+DYEE K +    A+ H +    +  ++ S     GA ++
Sbjct: 443 PWLTARNSCPLCRYELPTDDKDYEEGKLN----ASIHEIPQQDLSDDSSSDTTD-GAEAD 502

Query: 484 FESSQEEDYRNIDSAVISSNNSGR-GGRRRWLFV-AAPIVGLVGIALMLWFGNPLCDQRV 543
                  + R +   V   N S R GGR RW F+ AAPI GLVG+AL+LW   P+ D+R 
Sbjct: 503 DTCEISPECRELADEVPVRNGSDREGGRGRWFFLAAAPIAGLVGMALVLWLRIPVTDRRG 562

Query: 544 PGGQLTDGRQLDARSAESS--NQRENRTRRWW 557
             G+     Q    S+ SS  NQRENR+RRWW
Sbjct: 563 AIGRSNIQGQTQLHSSGSSLPNQRENRSRRWW 587

BLAST of MELO3C025263 vs. TrEMBL
Match: B9T0P5_RICCO (Zinc finger protein, putative OS=Ricinus communis GN=RCOM_0126460 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 9.0e-140
Identity = 283/582 (48.63%), Postives = 366/582 (62.89%), Query Frame = 1

Query: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVLDSDATSEPEPIDICG 60
           MD   +R  ++ P S     ++    V+  + +    C  C+R    D  ++ E I ICG
Sbjct: 1   MDTYSERTSQDVPLSTSRGHVDGGLVVE--NSVHPAACVLCRRTFSPD-NNDLEAISICG 60

Query: 61  DCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISS 120
           DCKFLLLED+     DS  R   RG RT  +S ES +NLFSQ+  HMINLVRQ QS++  
Sbjct: 61  DCKFLLLEDLGDSTHDSLRRRRRRGGRTRYNSFESLDNLFSQQLLHMINLVRQNQSTVPG 120

Query: 121 HGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGP 180
           H  +  DG  +V  + H+SS TTP  SRRWR+V SD+E++G D+FDS +GE+E+  S   
Sbjct: 121 HEIQSVDGDASVRLLHHTSSLTTPTGSRRWRRVLSDTESEGFDNFDSPYGENETTPSASW 180

Query: 181 YRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIGNW 240
           YRH  GDSD +S   YGGDSD S+DGHSFLDTD+F  P EGS  D+DTDIDPM AG+  W
Sbjct: 181 YRH--GDSDAISFSAYGGDSDVSVDGHSFLDTDMFFQPEEGSNLDSDTDIDPMHAGLNQW 240

Query: 241 DSSDEED-------DHGEFTEAETEQEIGS-SEARPQLQNSNVSSASRRGNSSNWTEQLS 300
           +S DEE+       D GE+ EA+ E++      A PQLQN   SS +   +S N  ++  
Sbjct: 241 NSDDEEEEEEELEEDDGEWEEADIEEDTNELGGAGPQLQNLLFSSPNESNHSINRRQRFD 300

Query: 301 SPEYEGSTPGHIRRNRRFYS---ITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTS 360
           SPE+E      IR  R+ Y+     N  + EL  YVG+S DYLD +GFE+LLE +AET S
Sbjct: 301 SPEFESLIRWRIRPGRQTYNRDIFANLEEPELPQYVGNSRDYLDARGFEELLEHLAETDS 360

Query: 361 SRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCI 420
           SRRGAPPAAVSFV +LP +++++EH KHD ++CAICKD L +G EVNQLPC HLYHP+CI
Sbjct: 361 SRRGAPPAAVSFVNSLPLVIVNEEHEKHDGLACAICKDVLSIGTEVNQLPCFHLYHPSCI 420

Query: 421 LPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPS-GLNGVGAN 480
           LPWLSARNSCPLCR+ELPTDD+DYEE KRS+ N    H +Q   V  ++ S   +G  A+
Sbjct: 421 LPWLSARNSCPLCRFELPTDDKDYEEGKRSNSNRMGIHEIQQQDVSEDSSSDDSDGAEAH 480

Query: 481 SEFESSQ----EEDYRNIDSAVISSNNSGRGGRRRWLFV-AAPIVGLVGIALMLWFGNPL 540
              E  Q    + +  ++D AV +S   G G  RRW F+ AAPIVGLVGI L+ W G+P 
Sbjct: 481 EAHEFDQGGIDQREVLDVDPAVNTSGREGSG--RRWFFLAAAPIVGLVGIVLVYWLGSPQ 540

Query: 541 -------CDQRVPGGQLTDGRQLDARSAESSNQRENRTRRWW 557
                  CD  VP   L    Q+ A  + + +QR NR+RRWW
Sbjct: 541 RRGPTHHCD--VPQRGL---HQIRAPGSSAPSQRGNRSRRWW 570

BLAST of MELO3C025263 vs. TrEMBL
Match: A0A067JQM1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00621 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 4.5e-139
Identity = 279/577 (48.35%), Postives = 371/577 (64.30%), Query Frame = 1

Query: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVLDSDATSEPEPIDICG 60
           MD D +R  E+ P S VH  ++S   V +HS +   TC  C+R+   D T + E I ICG
Sbjct: 1   MDRDWERTSEDVPLSVVHGHVDSGL-VADHS-VHPATCVMCRRIFAPD-TDDLENISICG 60

Query: 61  DCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISS 120
           DCKFLLLED+      S  R   RGRR   SSSES ENLFSQ+ +HMIN+VRQ  S+ S 
Sbjct: 61  DCKFLLLEDVGDSTHGSLQRRPHRGRRNRYSSSESIENLFSQQLSHMINVVRQNLSTASG 120

Query: 121 HGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGP 180
           H ++  DG ++V  + H+SS TT   SRRWRQV SD+E++G D+ DS++GE E+  S   
Sbjct: 121 HDNQSVDGDSSVRLLAHTSSRTTSAGSRRWRQVLSDTESEGFDNLDSVYGEIETTPSVSW 180

Query: 181 YRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIGNW 240
           +R  +GDSD +S   YGGDSD S+DGHSFLDT++FI P +GS  ++DTDIDPM AG+ +W
Sbjct: 181 HRAYHGDSDAISFSAYGGDSDVSIDGHSFLDTEMFIQPDDGSNFESDTDIDPMHAGLNHW 240

Query: 241 DSSDEED--------DHGEFTEAETEQE-IGSSEARPQLQNSNVSSASRRGNSSNWTEQL 300
           +  DEE+        D GE+ EA+ E++ I    A  QLQN  + S +    S+NW  Q 
Sbjct: 241 NLDDEEEEEEEEEEEDDGEWEEADIEEDTIQPVVAGSQLQNYLIPSPNEGNGSNNWHRQF 300

Query: 301 SSPEYEGSTPGHIRRNRRFYSITNYAQ---SELLSYVGDSGDYLDRQGFEDLLEQIAETT 360
            SP +E       R++R+  +   +A    SE+  YVG+SGDYLD +GFE+LL+ +AET 
Sbjct: 301 DSP-FEALINWRRRQSRQASNRDIFAHLEASEVPRYVGNSGDYLDARGFEELLQHLAETD 360

Query: 361 SSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTC 420
           +SRRGAPPAA+SFV +LP +VI++EH+ HD ++CAICKD L +G EVNQLPCLH+YHP+C
Sbjct: 361 NSRRGAPPAAMSFVNSLPLVVINEEHMTHDGLTCAICKDILSIGTEVNQLPCLHVYHPSC 420

Query: 421 ILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGAN 480
           ILPWLS RNSCPLCR+ELPTDD+DYEE K+S+ N    H +Q      E+ S  + V   
Sbjct: 421 ILPWLSTRNSCPLCRFELPTDDKDYEEGKQSNSNRIGIHEVQQQDDASEDSS--SDVSDE 480

Query: 481 SEFESSQEEDYRNIDSAVISSNNSGR-GGRRRWLFV---AAPIVGLVGIALMLWFGNPLC 540
           +E +   E + R +     + ++SGR G RRRW F+   AAPIV LVGI L+ W GNP  
Sbjct: 481 AEADEVGETEQRQVLDPHPAVSSSGREGSRRRWFFLAAAAAPIVSLVGIVLVFWLGNPQG 540

Query: 541 DQRVPGGQLTDGR---QLDARSAESSNQRENRTRRWW 557
             R     +   R   Q+   S+ + NQR NR+RRWW
Sbjct: 541 RGRPIRHCILPERGINQIQVPSSSAPNQRGNRSRRWW 571

BLAST of MELO3C025263 vs. TAIR10
Match: AT5G01980.1 (AT5G01980.1 RING/U-box superfamily protein)

HSP 1 Score: 345.1 bits (884), Expect = 7.9e-95
Identity = 227/533 (42.59%), Postives = 296/533 (55.53%), Query Frame = 1

Query: 38  CAFCQRVLDSDATSEPEPIDICGDCKFLLLEDIETPVRDSYHRMTPRGRR-------TMR 97
           C  C RV+ S A+ + E   +C DCKFLLLED  TP      R T R RR       T  
Sbjct: 24  CNLCGRVVQS-ASDDLEIFSLCVDCKFLLLEDFGTPAPPLARRQTIRRRRMRRRRRTTRH 83

Query: 98  SSSESPE--NLFSQEFTHMINLVRQRQSSISSHGDRYADGSNAVDFMQHSSSHTTPNQSR 157
            SSES    +L SQ+FTH+I++ RQ  S++ +  D   D          +SSHTTP+ S 
Sbjct: 84  DSSESVGVGDLSSQQFTHLISIARQSLSTVQASEDLRDD--------LRTSSHTTPSGST 143

Query: 158 RWRQVYSDSENDGLDSFDSMFGESESNFSFGPYRHSYGDSDVVSNRTYGGDSDASLDGHS 217
           RWR ++S+SE+D  D+F    GE+ESN SF  YR  + D+D +S   YGG+SDAS D H+
Sbjct: 144 RWR-LFSESESDDFDNF----GETESNASFSLYRFPHADNDTISFSAYGGESDASTDRHA 203

Query: 218 FLDTDIFILPGEGSDTD--TDIDPMRAGIGNWDSSDEEDDHGEFTEAETEQEIGSSEARP 277
               DIF+ P + SD D  TDIDPMRAG+  W+S DEED        E E+  G S    
Sbjct: 204 ----DIFVQPDDRSDIDFDTDIDPMRAGLNQWNS-DEED-------REWEEGAGPSGVAG 263

Query: 278 QLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPGHIRRNRRFYS---ITNYAQSELLSYV 337
               + ++S S   +S     +  SPE E S    I   R   S    T     +   Y 
Sbjct: 264 TRYRNYLASPSESYSSMT---RFDSPELERSFRQRIIERRHSLSRNIFTGLEDLDFSPYA 323

Query: 338 GDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 397
            + GDYLD +GF++LLEQ+AE+ +SRRGAPPA+VS V+NLPR++I++EH+    + CAIC
Sbjct: 324 ANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVIIAEEHVMKGLV-CAIC 383

Query: 398 KDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAA 457
           K+   +  E  QLPCLHLYH  CI+PWLSARNSCPLCRYELPTDD+DYEE KR+ ++   
Sbjct: 384 KELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEEGKRNVLD--- 443

Query: 458 THALQPWVVGLENPSGLNGVGANSEFESSQEEDYRNIDSAVISSNNSGRGGRRRWLFV-A 517
                   V  ++ S  +G       ES +EE     +    S +   R  R RWLF+ A
Sbjct: 444 --------VSEDSSSSDDGT------ESGEEEYVERGE----SESGVSRVSRGRWLFLAA 489

Query: 518 APIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQLDARSAESSNQRENRTRRW 556
           AP+V LVG+ L +W  +P              R +    A S +QRENRTRRW
Sbjct: 504 APVVSLVGVVLAMWLSSP------------HRRGI----AISHSQRENRTRRW 489

BLAST of MELO3C025263 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-27
Identity = 53/115 (46.09%), Postives = 73/115 (63.48%), Query Frame = 1

Query: 324 GDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAIC 383
           G+ GDY    G E L++Q+AE   +R G PPA+ S +  LP + ++K+ LK +   CA+C
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 384 KDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSS 439
            D    G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD DYE   + S
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGS 274

BLAST of MELO3C025263 vs. TAIR10
Match: AT5G08139.1 (AT5G08139.1 RING/U-box superfamily protein)

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-26
Identity = 89/296 (30.07%), Postives = 135/296 (45.61%), Query Frame = 1

Query: 160 DGLDSFDSMFGESESNFSFGPYRHSYGDSDVVSNRTYGGDSDA-----SLDGHSFLDTDI 219
           D  D  D   G ++    F     S  D  +++  +   D D       L G    + D+
Sbjct: 78  DEPDDDDFFVGRTDFGLEFRDIASSGNDIRLITVESGSDDDDGVENERELWGIDLNEEDV 137

Query: 220 FILPGEGSDTDTDIDPMRAGIGN-WDSSDEED-----DHGEFTEA--------ETEQEIG 279
           ++      D + + D +   I   WDS   ED     D  ++ E         + E+EI 
Sbjct: 138 YV----NDDDEYEDDDVSVTIPLCWDSLQLEDREVTADEFDWEEVGGGGGGGVDDEREIR 197

Query: 280 SSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPGHIRRNRRFYSITNYAQSELL 339
           S  A+  + + ++ SAS   +      +    E  G+    +  N       +    EL 
Sbjct: 198 SGFAQIDMNDESLISASPIISLEGLVTR-ERAEGSGNLGWEVLLNHTLEINFDVDNREL- 257

Query: 340 SYVG-DSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSIS 399
            Y+G D  DY+  Q ++ L EQ A+   S  G PP + SF+ NLP +++  E+     + 
Sbjct: 258 -YIGGDHDDYV--QDYDMLFEQFADAEVSVIGLPPTSKSFLNNLPVVLLEGENDDDGGLV 317

Query: 400 CAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVK 436
           CA+CKD + +G +  QLPC H YH  CI+PWL  RN+CP+CRYELPTDD +YE+ K
Sbjct: 318 CAVCKDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDDAEYEQRK 364

BLAST of MELO3C025263 vs. TAIR10
Match: AT5G64920.1 (AT5G64920.1 COP1-interacting protein 8)

HSP 1 Score: 116.3 bits (290), Expect = 6.0e-26
Identity = 70/210 (33.33%), Postives = 102/210 (48.57%), Query Frame = 1

Query: 242 DEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPGH 301
           +EED+    T  + E E      R +   +   S + R    +W E L   E + S    
Sbjct: 131 EEEDEEENLTVNDEEDEEDDLRRRNRFPLTTTQSRTGRNRILDWAEILMGIE-DNSIEFR 190

Query: 302 IRRNRRFYSITNYAQSELLSYVGDSGDYLD-RQGFEDLLEQIAETTSS----RRGAPPAA 361
           +  +R               Y G+  DY+D   G+E LL+ +AE        RRGAPPAA
Sbjct: 191 MESDR---------------YAGNPADYIDDAAGYEALLQNLAEGDGGGGGGRRGAPPAA 250

Query: 362 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 421
            S ++ L    +S    +   + CA+CKD +++G    +LPC H YH  CI+PWL  RNS
Sbjct: 251 KSAIEALETFEVSSSEGEMVMV-CAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLGTRNS 310

Query: 422 CPLCRYELPTDDRDYEEVKRSSINTAATHA 447
           CP+CR++L TDD +YEE ++   +T +  A
Sbjct: 311 CPVCRFQLETDDAEYEEERKKRTSTVSDSA 323

BLAST of MELO3C025263 vs. TAIR10
Match: AT1G55530.1 (AT1G55530.1 RING/U-box superfamily protein)

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-25
Identity = 78/269 (29.00%), Postives = 121/269 (44.98%), Query Frame = 1

Query: 229 DPMRAGIGNWDSSDEEDDHGEFTEAETEQEIGSSEARPQLQ--------NSNVSSASRRG 288
           DP+R    N      ED+  E  + E  ++ G ++   QLQ        +S       +G
Sbjct: 70  DPVRRR-RNQSVESVEDNQNE-VQTENNEDDGENDLDWQLQEILRRRRRHSAAVLQLLQG 129

Query: 289 NSSNWTEQLSSPEYEGSTPGH---IRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFED 348
             +  + +  S    G+ PG    I  + +  ++ N A  + +   G  GDY    GFE 
Sbjct: 130 IRAGLSVESESTGNGGNNPGRVILINTSNQTITVQNSADMDSVP-AGSLGDYFIGPGFEM 189

Query: 349 LLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLP 408
           LL+++AE   +R G PPA    V+ L  + I       +++ C++C D   +G E   +P
Sbjct: 190 LLQRLAENDPNRYGTPPAKKEAVEALATVKIE------ETLQCSVCLDDFEIGTEAKLMP 249

Query: 409 CLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENP 468
           C H +H  C+LPWL   +SCP+CRY+LP D+   + V  +S N  ++ A      G EN 
Sbjct: 250 CTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSVTTTSDNNGSSSASATTSHGAENS 309

Query: 469 SGLNGVGANSEFESSQEEDYRNIDSAVIS 487
            G        E E  +EE+  N D +  S
Sbjct: 310 DGNRRQEEEEEEEEEEEEENENNDGSGFS 329

BLAST of MELO3C025263 vs. NCBI nr
Match: gi|659126662|ref|XP_008463301.1| (PREDICTED: uncharacterized protein LOC103501491 [Cucumis melo])

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 1

Query: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVLDSDATSEPEPIDICG 60
           MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVLDSDATSEPEPIDICG
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVLDSDATSEPEPIDICG 60

Query: 61  DCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISS 120
           DCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISS
Sbjct: 61  DCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISS 120

Query: 121 HGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGP 180
           HGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGP
Sbjct: 121 HGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGP 180

Query: 181 YRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGSDTDTDIDPMRAGIGNWDS 240
           YRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGSDTDTDIDPMRAGIGNWDS
Sbjct: 181 YRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGSDTDTDIDPMRAGIGNWDS 240

Query: 241 SDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPG 300
           SDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPG
Sbjct: 241 SDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGSTPG 300

Query: 301 HIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFV 360
           HIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFV
Sbjct: 301 HIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSSRRGAPPAAVSFV 360

Query: 361 KNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLC 420
           KNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLC
Sbjct: 361 KNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNSCPLC 420

Query: 421 RYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEEDYRNI 480
           RYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEEDYRNI
Sbjct: 421 RYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEEDYRNI 480

Query: 481 DSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQLDAR 540
           DSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQLDAR
Sbjct: 481 DSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQLDAR 540

Query: 541 SAESSNQRENRTRRWW 557
           SAESSNQRENRTRRWW
Sbjct: 541 SAESSNQRENRTRRWW 556

BLAST of MELO3C025263 vs. NCBI nr
Match: gi|449449944|ref|XP_004142724.1| (PREDICTED: E3 ubiquitin-protein ligase Praja-2 [Cucumis sativus])

HSP 1 Score: 1067.4 bits (2759), Expect = 8.6e-309
Identity = 524/560 (93.57%), Postives = 539/560 (96.25%), Query Frame = 1

Query: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60
           MDIDLQRILEEFPQS VHVIMN+NQRVDEHSPIQLPTCAFCQRVL  DSDA SEPEPIDI
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120
           CGDCKFLLLEDIETPVRDSYHRMTPRGRR MRSSSESPENLFSQEFTHMIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180
           SSHGDRYADGSNA+DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDVVSNRTYGGDSDASLD-GHSFLDTDIFILPGEGSDTDTDIDPMRAGIGN 240
           GPYRHSYGDSDVVSNRTYGG+SD SLD GHSFLDTD+FILPGEGSDTDTDIDPMRAG+G+
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGSDTDTDIDPMRAGVGH 240

Query: 241 WDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYEGS 300
           WDSSDEEDDHGEFTEAETEQE+GSSEARPQLQN +VSSASRRGNSSNW EQLSSPEYEGS
Sbjct: 241 WDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYEGS 300

Query: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAET-TSSRRGAPPAA 360
           TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFE+LLEQIAET TSSRRGAPPAA
Sbjct: 301 TPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLDRQGFEELLEQIAETTTSSRRGAPPAA 360

Query: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCILPWLSARNS 420
           VSFVKNLPRLVISKEHLKHDSISCAICKDFL +GVEVNQLPCLHLYHP+CILPWLSARNS
Sbjct: 361 VSFVKNLPRLVISKEHLKHDSISCAICKDFLHLGVEVNQLPCLHLYHPSCILPWLSARNS 420

Query: 421 CPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSEFESSQEED 480
           CPLCRYELPTDDRDYEEVK+SSIN AA H LQP VVGLENPSGLNGVGAN EFE+SQEED
Sbjct: 421 CPLCRYELPTDDRDYEEVKQSSINAAAVHGLQPRVVGLENPSGLNGVGANPEFETSQEED 480

Query: 481 YRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVPGGQLTDGRQ 540
            RNIDSAVISSNNSGRG RRRWLFVAAPIVGLVGIALMLWFGNPLCDQRV GGQLTDGRQ
Sbjct: 481 QRNIDSAVISSNNSGRGARRRWLFVAAPIVGLVGIALMLWFGNPLCDQRVSGGQLTDGRQ 540

Query: 541 LDARSAESSNQRENRTRRWW 557
           LDA SAESSNQRENR+RRWW
Sbjct: 541 LDAHSAESSNQRENRSRRWW 560

BLAST of MELO3C025263 vs. NCBI nr
Match: gi|1009159440|ref|XP_015897813.1| (PREDICTED: E3 ubiquitin-protein ligase Praja-2 [Ziziphus jujuba])

HSP 1 Score: 521.5 bits (1342), Expect = 1.7e-144
Identity = 293/586 (50.00%), Postives = 371/586 (63.31%), Query Frame = 1

Query: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDI 60
           MD + QRI +EFP S     ++ +Q          P C+ CQ++L  D++   + E I I
Sbjct: 1   MDGESQRISQEFPHSTAQSGLSGSQP---------PRCSLCQKILSPDTEVAGDLESIGI 60

Query: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTM-RSSSESPENLFSQEFTHMINLVRQRQSS 120
           CGDCKFLLLED  TP  +S+HR  P  RRT   SSSES ENLFSQ+F+ MINL RQ QSS
Sbjct: 61  CGDCKFLLLEDFGTPTHESHHRRPPSRRRTRYNSSSESIENLFSQQFSQMINLARQTQSS 120

Query: 121 ISSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFS 180
           +S   D   DG       Q +SS   P  S RWR+V SD+E+DG D+ DS++ ESESN S
Sbjct: 121 VSGLEDSPGDGDATTRLPQQTSSRAAPRGSTRWRRVLSDTESDGFDNADSLYAESESNVS 180

Query: 181 FGPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGSDTD--TDIDPMRAGI 240
           FG YR  + +SD VS  TYGGDSDAS+D H FLD+++F  P EGSD D  TDIDPM AG+
Sbjct: 181 FGRYRVFHAESDAVSFSTYGGDSDASVDLHGFLDSEMFTQPNEGSDFDSETDIDPMHAGL 240

Query: 241 GNWDSSDEEDDHG---EFTEAETEQEIG-------------SSEARPQLQNSNVSSASRR 300
             W+S + E++ G   E  E E E+E G             S+EAR + QN   SS S  
Sbjct: 241 NQWNSDELEEEGGGGEEGEEEEGEEEDGEWEEVDGEENTVDSTEARARFQNFLFSSQSES 300

Query: 301 GNSSNWTEQLSSPEYEGSTPGHIRRNRRFYS---ITNYAQSELLSYVGDSGDYLDRQGFE 360
             +  W+ Q  SPE EG    + R N + Y+    +N  QSE L Y+G+SGDYLD +GFE
Sbjct: 301 NYAVGWSRQTHSPESEGMI--NWRANSQLYAYDIFSNLEQSEFLPYIGNSGDYLDARGFE 360

Query: 361 DLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQL 420
           DLL+ +AE  +SRRGAPPA+VS V +LPR+VI+KEH KHD ++CAICKD L +G EVNQL
Sbjct: 361 DLLQHLAEADNSRRGAPPASVSCVNSLPRVVINKEHKKHDDLACAICKDVLSIGTEVNQL 420

Query: 421 PCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLEN 480
           PC HLYHP+CILPWLSARNSCPLCRYELPTDDR+YEE K++ IN    H +Q   VG ++
Sbjct: 421 PCKHLYHPSCILPWLSARNSCPLCRYELPTDDREYEEGKQNIINRGQMHDVQQ-DVGEDS 480

Query: 481 PSGLNGVG---ANSEF--ESSQEEDYRNIDSAVISSNNSGRGGRRRWLFV-AAPIVGLVG 540
            S ++ +       EF  + S++ +  +I+ A+ SS      G  RW F+ AAPIVGLVG
Sbjct: 481 SSDVSVIAEEDGEHEFSRDISEQREVLDIEPAINSSVRESSTG--RWFFLAAAPIVGLVG 540

Query: 541 IALMLWFGNPLCDQRVPGGQLTDGRQLDARSAESSNQRENRTRRWW 557
           I L+ W GNPL + R        G+Q    S  S NQRENR+RRWW
Sbjct: 541 IVLVFWLGNPLGESRGAARLPEQGQQQILVSGSSLNQRENRSRRWW 572

BLAST of MELO3C025263 vs. NCBI nr
Match: gi|147816583|emb|CAN75069.1| (hypothetical protein VITISV_040928 [Vitis vinifera])

HSP 1 Score: 518.8 bits (1335), Expect = 1.1e-143
Identity = 284/572 (49.65%), Postives = 372/572 (65.03%), Query Frame = 1

Query: 4   DLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDICGD 63
           D + + +E  QS V    NS+  ++ HS +    CA CQ++L  D + T E EPI +CGD
Sbjct: 81  DSRSVPQELSQSTVPNSSNSDSLLN-HSDLPA-ACALCQKILLPDDEGTGELEPISMCGD 140

Query: 64  CKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISSH 123
           CKFL LED+ +P +DS+ R  PR RRT  SSSES ENLFSQ F+HMINLVRQ QS++S H
Sbjct: 141 CKFLFLEDLRSPAQDSHWRRPPRVRRTRYSSSESIENLFSQHFSHMINLVRQNQSTVSGH 200

Query: 124 GDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGPY 183
            D+  D       +Q  SS TTP+ SRRWR+V SD+E+DG D+ DS++GESESN S+G Y
Sbjct: 201 EDQLVDVDPGARLLQRPSSRTTPSGSRRWRRVPSDTESDGFDNLDSLYGESESNISYGGY 260

Query: 184 RHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIGNWD 243
           R   G+SD +S   YGGDSDAS+DGHSFLDT++++ P +GS  ++DTDIDPM A +  W+
Sbjct: 261 RLFNGESDAISFSAYGGDSDASVDGHSFLDTEMYVQPDDGSNLNSDTDIDPMHARLSQWN 320

Query: 244 SSDEEDDHGEFTEAETEQE--------IGSSEARPQLQNSNVSSASRRGNSSNWTEQLSS 303
           S D+++D  E  E + E E        + S+EA    +N   SS++      N   ++ S
Sbjct: 321 SDDQDEDDDEEXEEDDEWEEADPEENVVESAEAEVHPRNFFPSSSNGNNVLVNMRRRIHS 380

Query: 304 PEYEGSTPGHIRRNRRFY---SITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSS 363
           PE+ G+ P  +R  R+ +     +N  +SE+L Y G+S DYLD +GFE+ LE +AET SS
Sbjct: 381 PEFLGAIPWRLREGRQTFISNFFSNLDESEVLPYFGNSEDYLDARGFEEFLEHLAETDSS 440

Query: 364 RRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCIL 423
           RRGAPPAAVSFV +LPR+VI++EH K D + CAICKD L VG EVNQLPC HLYHP CIL
Sbjct: 441 RRGAPPAAVSFVNSLPRVVINEEHEKRDGLVCAICKDVLSVGTEVNQLPCFHLYHPYCIL 500

Query: 424 PWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSE 483
           PWL+ARNSCPLCRYELPTDD+DYEE K +    A+ H +    +  ++ S     GA ++
Sbjct: 501 PWLTARNSCPLCRYELPTDDKDYEEGKLN----ASIHEIPQQDLSDDSSSDTTD-GAEAD 560

Query: 484 FESSQEEDYRNIDSAVISSNNSGR-GGRRRWLFV-AAPIVGLVGIALMLWFGNPLCDQRV 543
                  + R +   V   N S R GGR RW F+ AAPI GLVG+AL+LW   P+ D+R 
Sbjct: 561 DTCEISPECRELADEVPVRNGSDREGGRGRWFFLAAAPIAGLVGMALVLWLRIPVTDRRG 620

Query: 544 PGGQLTDGRQLDARSAESS--NQRENRTRRWW 557
             G+     Q    S+ SS  NQRENR+RRWW
Sbjct: 621 AIGRSNIQGQTQLHSSGSSLPNQRENRSRRWW 645

BLAST of MELO3C025263 vs. NCBI nr
Match: gi|731398020|ref|XP_010653103.1| (PREDICTED: E3 ubiquitin-protein ligase Praja-2 [Vitis vinifera])

HSP 1 Score: 518.8 bits (1335), Expect = 1.1e-143
Identity = 284/572 (49.65%), Postives = 372/572 (65.03%), Query Frame = 1

Query: 4   DLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVL--DSDATSEPEPIDICGD 63
           D + + +E  QS V    NS+  ++ HS +    CA CQ++L  D + T E EPI +CGD
Sbjct: 3   DSRSVPQELSQSTVPNSSNSDSLLN-HSDLPA-ACALCQKILLPDDEGTGELEPISMCGD 62

Query: 64  CKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSISSH 123
           CKFL LED+ +P +DS+ R  PR RRT  SSSES ENLFSQ F+HMINLVRQ QS++S H
Sbjct: 63  CKFLFLEDLRSPAQDSHWRRPPRVRRTRYSSSESIENLFSQHFSHMINLVRQNQSTVSGH 122

Query: 124 GDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSFGPY 183
            D+  D       +Q  SS TTP+ SRRWR+V SD+E+DG D+ DS++GESESN S+G Y
Sbjct: 123 EDQLVDVDPGARLLQRPSSRTTPSGSRRWRRVPSDTESDGFDNLDSLYGESESNISYGGY 182

Query: 184 RHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGS--DTDTDIDPMRAGIGNWD 243
           R   G+SD +S   YGGDSDAS+DGHSFLDT++++ P +GS  ++DTDIDPM A +  W+
Sbjct: 183 RLFNGESDAISFSAYGGDSDASVDGHSFLDTEMYVQPDDGSNLNSDTDIDPMHARLSQWN 242

Query: 244 SSDEEDDHGEFTEAETEQE--------IGSSEARPQLQNSNVSSASRRGNSSNWTEQLSS 303
           S D+++D  E  E + E E        + S+EA    +N   SS++      N   ++ S
Sbjct: 243 SDDQDEDDDEEEEEDDEWEEADPEENVVESAEAEVHPRNFFPSSSNGNNVLVNMRRRIHS 302

Query: 304 PEYEGSTPGHIRRNRRFY---SITNYAQSELLSYVGDSGDYLDRQGFEDLLEQIAETTSS 363
           PE+ G+ P  +R  R+ +     +N  +SE+L Y G+S DYLD +GFE+ LE +AET SS
Sbjct: 303 PEFLGAIPWRLREGRQTFISNFFSNLDESEVLPYFGNSEDYLDARGFEEFLEHLAETDSS 362

Query: 364 RRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGVEVNQLPCLHLYHPTCIL 423
           RRGAPPAAVSFV +LPR+VI++EH K D + CAICKD L VG EVNQLPC HLYHP CIL
Sbjct: 363 RRGAPPAAVSFVNSLPRVVINEEHEKRDGLVCAICKDVLSVGTEVNQLPCFHLYHPYCIL 422

Query: 424 PWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWVVGLENPSGLNGVGANSE 483
           PWL+ARNSCPLCRYELPTDD+DYEE K +    A+ H +    +  ++ S     GA ++
Sbjct: 423 PWLTARNSCPLCRYELPTDDKDYEEGKLN----ASIHEIPQQDLSDDSSSDTTD-GAEAD 482

Query: 484 FESSQEEDYRNIDSAVISSNNSGR-GGRRRWLFV-AAPIVGLVGIALMLWFGNPLCDQRV 543
                  + R +   V   N S R GGR RW F+ AAPI GLVG+AL+LW   P+ D+R 
Sbjct: 483 DTCEISPECRELADEVPVRNGSDREGGRGRWFFLAAAPIAGLVGMALVLWLRIPVTDRRG 542

Query: 544 PGGQLTDGRQLDARSAESS--NQRENRTRRWW 557
             G+     Q    S+ SS  NQRENR+RRWW
Sbjct: 543 AIGRSNIQGQTQLHSSGSSLPNQRENRSRRWW 567