MELO3C021725 (gene) Melon (DHL92) v3.5.1

NameMELO3C021725
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionSubtilisin-like protease
Locationchr12 : 16129806 .. 16134209 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGGTATGTCACCACGTAACATTAATATCCATTCGTTAATTTAAAATAATTATGAAGCAGAAAATCATATCTAATTACTTTCATCTTCCTCAGGACCCAGTTTAGGGAGTGAGTTTTTCCTACATAAGTTGGTATTGGCTAAGGATGCAAAGATGCAAAATGAGACACAATATGACGACATACCAAGTTACATTGAAGAATGCCAAAATCCTGAAGCTTTTGACCCAAATATTGTCCAAAACAGCATAGTCCTTTGCTCTTTCTCACAAGGATTCCTCAATGGAACCTCCTCTCTTGCAGCCATTATTCACACTGCAAAAAAACTCAAATTCATGGGTTTTGTTCTCATTGCAAATCCAAACTATGGTGATTTCATTGCAGAGCCTATCCCTTTCAGAGTTCCCGGTATTCTCGTCCCCAGCGTATCAGATACTCAGGTATATCTTTACAACATATAAACAAATAAAAATAGCTATTCGAATCAAAATCTTCATTTTTGAATTTTAAGTTTTTATTCATCAAAATGGCCAAACGAAAAAAAAAGAAAAAAAAGAAAAGAAAGAAAGAGAGCTCCATGAAATCAAGTTTATATACTTACCCCTGTAAGTTTATTTATTTTGTTTTCGACTTTTATCCAATATTTCCATAAACAAGAACTAAAGAAAAATTAGTTTGTTAAAACTTAATTGCTTTTGTTATTAGAATGTGATTAAAATTCAAAGGTGAAAACCTTGCACCAACACTAGCATGCGTCCATCCATCTCTGACTTGTGCCATACATAATTATTGGACACTCCAATACACTTGTTGAATACATATCAAACATTTGTTACGTTAGTGCAATATGTATGTTAGACATGCATAAACATTTGTTAAGCAGACTAGAGAGACTAGAAAGACACATGTATCATATAGTAATAAAAAATTTTGAGTGTAAAATACATCAAATTAAGTTTTTGAAGCATAACTAGATTTTTAAATATGACATGCTACCATGTTTGTATCAGGTTATCCTTCTTTCTTGTATTTGTATTTGTGCTTCTCAGGTGAAAATCATAGTTACGTATAAGGGATTTTATGAAAAACAACAAAAAAAATTAAAAACAAAATTATTATCAAAGGGACCAAAAATTTTAAATGAACAGGACCTGACAATCTTGGATTGCAAGCCAAAAATATAGGTAATACTGAAGTACTATGAAGAAAATACATGCAAGGATGGAAGAGGAATGGTTAGAGAGTTTAAAGGGAAAGCAGGCATTGGAGAAGGAAGAATTGCTTCATTTGGAAAGGAAGCACCCACAGTAAGCAGATTTTCATCAAGAGGACCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCTGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTGAAGGGTCAGCTTTTCATGAACTTATACTGTTCTTTTAGTTTCATCTATTTTTCCCCTTTTACAATTGTCATTCAGTCTATATGAATTTTAAGCAATTCTTGTTTTGGTCTCCTTAGGTTTTAAAACCCCAGTGCTCAAAAACAAAATTTGTTTTTAGTTTTCTCTTTAATTATGAAATTTATATATACTGTTTTTCTCTTCAATTTTATATTTGGTTCTTACATTTATGATACAAACACTCAGTTATCGGTAAACTTTTATGACGTTTTATGACGTTTTAAACTCTCTTAATTTAGTTCTTACAATTAAACTCTACTTTCTCTTAATTTTTTATGATGTTTTCCATCTCTCTTGAGTAAGAGTTGAACTTCTTATCAAATTCTATAGACAAGAACAAGTTCTTGAAAACTAATCCTTCTGGTTTTCAAGACTTTGAAACCTAGTTTTCGAAAACAACAATAAAAGATAGATAACAAAACAAAAAGTTTAGAGGTGGAAGAAGTGTTTATCTGCATGCTTTTAACTTTTAAAAGTTAAAAATTGAAAACCAAGTTGTCAAATAGATTTTTTTGAATTAGTTTTTAAAATTTGTTTTGACTTTTAAAAATATCAATGGAAAGTAAATTACAATCAAAAAATAAAAAAAAAAACATATAGATGATCGAACTAGTATTTATTATTAGGTTAATTTTAAAAAACAGAAAAAAAGAAAAAGTACAACAAAAGAATTAGTAAATATGGCCTACGTCCTTCTAAATTTCATTAAATTTCACCTTTACAAGTCCCAATCATCTCAAGATCTTGAAGTTAGATAAGGGACTAAGAAGTTCAATTTTAGAAGTAAAAGGACAAAAACAAGAAGTATATGAAAGCTCTATAACCAGATAATATTTATTTTCTTGAAGTTGCAAAACATAATCAAAATCATAGTTATGTCGTATCAAAAGGGCACAAGAGAAGTTGATTGAAACATGAGTGTTGAATTACATATATATATATATATATATAGGATACCGTTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAGTAGCAGCACTTATCAAGCAAAAGTATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTTGATTTCGGCGCTGGGCTCGTTTCTCCGACCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGGTAAATTCTGATATACATACTCTACCTTTTCATTATTTGGGCAACTGTGTTTGTTGAATATCGCAAAACTGCTACTTAATCACGCTTTGAAATGTTTGTGGTAGAGTAAAAAAAAAAAACTAACCAACTAATGTTTTCTTAAAGTCTAACCGTGCTTGTGTTAGGATACCACTTAACATGAAAGCTTAGAGGAATACAAAGAAACATGAACATAGTAAAATATTACAATATCAAGAGATGTTACAACGTATACAGTCTTTCGAGAGGGATATCTCTCTTCCAAGACCTTATAATGGAAGCTTCCCACACAACTAATTCACACCCTCCTATGGTCCTTAACTCTCCTTATTTATAATCATGTCACTCTCACAGCTAATTATAACTCTATGCCCTTTCTAACATCATATTAATATTATCCTAGGCCTATAACTATACTTAATTTTTTAAGAGAGGCATTTTGCTGATGGATGAAATAAGGAAAAACCCCAAATACACATATAGGCGATTACTAGAAGAAAGCTCTCCACAAGTGGAAAGTAAAGGAAAGTAAATCCTTTGATTTGCACCAAACAAACATAGTAGATAGAATTAAAAACATAGAGATGAAATCCCTGGAAAGTCGTCCATTACGTTTCTCCACATAAAGTCAAAAAATTTAAAAAAAAAAAAAAAAAAAATTAAACGCTCGCAAAATGGCCAACCGAAACATTCTTCTATGGACGTCTGACTAGTACTCTCAAAACTTGTTAAGTAAAGTGTTATCCTCAGAAAACAAAATGTGTATCAACACATTCTCTTAAAACAAACAAATAAAAAAGCAGGTCATCTTTTATGTAATTGTATCTATTTAGTTTTCAACATTAACTACCCAGTTAATTAATTATAATAATGAACACATTCTCTCTTAAAACAAACAAACAAATCAAAAGCAGATCCTTTTTCAATTCAGTTCCATGTTGTTCCACATCAACTTTTCCCCTTATTTAACAAACATTACAAGAAAAAAAAAAAACTAAACAAATATGTCCAATAAAAGTTTCAAAACATCAAAAGTAACAGACAACATAAACTAAGAATTATAAGAGAAACCAATATTGCAAATTTGTTTTGCCAACAGATTCAGTCTAAGCCAATTCAAAAATCAAGAGAACTAACCATATCTTCTTGAAACAGAATATGAAGACTATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTAGTCAAAAGCGCCACCGGAGGACAATGCAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCGGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGCTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAAACCGCCGTGGTTCACTGTAGCGGCTGAAGAAGTCCAAAACTTGGAAATACAAATCATTGCCACACACAAAACGGATCATTTTACCTTTGGGGAGAATTTTTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATTCTGAGGGTCAGGATTGGTTTGTTCCCTTTGAAAGTTTGTTTCTATACGTCCTTCATTTATCCCTCTCTCCATGTTTTCTTTTGA

mRNA sequence

ATGTCTGGACCCAGTTTAGGGAGTGAGTTTTTCCTACATAAGTTGGTATTGGCTAAGGATGCAAAGATGCAAAATGAGACACAATATGACGACATACCAAGTTACATTGAAGAATGCCAAAATCCTGAAGCTTTTGACCCAAATATTGTCCAAAACAGCATAGTCCTTTGCTCTTTCTCACAAGGATTCCTCAATGGAACCTCCTCTCTTGCAGCCATTATTCACACTGCAAAAAAACTCAAATTCATGGGTTTTGTTCTCATTGCAAATCCAAACTATGGTGATTTCATTGCAGAGCCTATCCCTTTCAGAGTTCCCGGTATTCTCGTCCCCAGCGTATCAGATACTCAGGTAATACTGAAGTACTATGAAGAAAATACATGCAAGGATGGAAGAGGAATGGTTAGAGAGTTTAAAGGGAAAGCAGGCATTGGAGAAGGAAGAATTGCTTCATTTGGAAAGGAAGCACCCACAGTAAGCAGATTTTCATCAAGAGGACCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCTGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTGAAGGGATACCGTTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAGTAGCAGCACTTATCAAGCAAAAGTATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTTGATTTCGGCGCTGGGCTCGTTTCTCCGACCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGAATATGAAGACTATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTAGTCAAAAGCGCCACCGGAGGACAATGCAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCGGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGCTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAAACCGCCGTGGTTCACTGTAGCGGCTGAAGAAGTCCAAAACTTGGAAATACAAATCATTGCCACACACAAAACGGATCATTTTACCTTTGGGGAGAATTTTTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATTCTGAGGGTCAGGATTGGTTTGTTCCCTTTGAAAGTTTGTTTCTATACGTCCTTCATTTATCCCTCTCTCCATGTTTTCTTTTGA

Coding sequence (CDS)

ATGTCTGGACCCAGTTTAGGGAGTGAGTTTTTCCTACATAAGTTGGTATTGGCTAAGGATGCAAAGATGCAAAATGAGACACAATATGACGACATACCAAGTTACATTGAAGAATGCCAAAATCCTGAAGCTTTTGACCCAAATATTGTCCAAAACAGCATAGTCCTTTGCTCTTTCTCACAAGGATTCCTCAATGGAACCTCCTCTCTTGCAGCCATTATTCACACTGCAAAAAAACTCAAATTCATGGGTTTTGTTCTCATTGCAAATCCAAACTATGGTGATTTCATTGCAGAGCCTATCCCTTTCAGAGTTCCCGGTATTCTCGTCCCCAGCGTATCAGATACTCAGGTAATACTGAAGTACTATGAAGAAAATACATGCAAGGATGGAAGAGGAATGGTTAGAGAGTTTAAAGGGAAAGCAGGCATTGGAGAAGGAAGAATTGCTTCATTTGGAAAGGAAGCACCCACAGTAAGCAGATTTTCATCAAGAGGACCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCTGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTGAAGGGATACCGTTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAGTAGCAGCACTTATCAAGCAAAAGTATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTTGATTTCGGCGCTGGGCTCGTTTCTCCGACCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGAATATGAAGACTATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTAGTCAAAAGCGCCACCGGAGGACAATGCAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCGGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGCTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAAACCGCCGTGGTTCACTGTAGCGGCTGAAGAAGTCCAAAACTTGGAAATACAAATCATTGCCACACACAAAACGGATCATTTTACCTTTGGGGAGAATTTTTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATTCTGAGGGTCAGGATTGGTTTGTTCCCTTTGAAAGTTTGTTTCTATACGTCCTTCATTTATCCCTCTCTCCATGTTTTCTTTTGA

Protein sequence

MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGENFFDRKFKPYCKNPFINFGCFCILRVRIGLFPLKVCFYTSFIYPSLHVFF*
BLAST of MELO3C021725 vs. Swiss-Prot
Match: SBT24_ARATH (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.3e-132
Identity = 235/413 (56.90%), Postives = 295/413 (71.43%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGP+LG+    H+LVLAKDA   N +    +   IEECQ PE FDP  V  SIV+C+FS
Sbjct: 402 LSGPTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSIVICTFS 461

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GF N  S++ AI  TA+ L FMGF+LIANP +GD++AEP+ F  PGIL+P+VS  Q+IL
Sbjct: 462 DGFYNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIIL 521

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYEE T +D RG+  +F  +A IGEGR + F  +AP VSRFSSRGP +I+  RS  DVL
Sbjct: 522 RYYEEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVL 581

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIW AWS  SA +P+L G  FA++SGTSMA PHI G+ ALIKQ  PSWTP+
Sbjct: 582 KPDILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPA 641

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASA+STTA +YD NG++I AE + L  L+PS  FD GAG V+P  ALDPGLV P  +E
Sbjct: 642 MIASAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFE 701

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYI+FLCSLP + PA ++ ATG  C  ++  SHPA+LN PS+TIS+L    VVRR  ++V
Sbjct: 702 DYISFLCSLPNISPATIRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDV 761

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             K ETY+ SV+PPNGTTV + P WFTV  ++ Q+L+I+   T   + FTFGE
Sbjct: 762 SNKTETYLGSVLPPNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGE 812

BLAST of MELO3C021725 vs. Swiss-Prot
Match: SBT25_ARATH (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.9e-83
Identity = 165/406 (40.64%), Postives = 241/406 (59.36%), Query Frame = 1

Query: 12  LHKLVLAKDAKMQNE-TQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSL 71
           L+ LV A D  + +  ++Y+  PS   +CQ PE F+  +V+ +I+LC +S  F+ GT+S+
Sbjct: 395 LYTLVSANDVLLDSSVSKYN--PS---DCQRPEVFNKKLVEGNILLCGYSFNFVVGTASI 454

Query: 72  AAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKD 131
             ++ TAK L   GFVL+          +P+P  +PGIL+  VS +  ++ YY  +T +D
Sbjct: 455 KKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDVSKSMDLIDYYNASTSRD 514

Query: 132 GRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQ 191
             G V+ FK +  IG+G      K AP V+ FS+RGP+  + +   AD+LKPDILAPG+ 
Sbjct: 515 WTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYL 574

Query: 192 IWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTA 251
           IWAAW P    EP   G  FAL+SGTSMAAPHI G+AAL+KQK+P W+P+ I SA+ TT+
Sbjct: 575 IWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTS 634

Query: 252 TKYDMNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLC 311
           T  D  G L+QA+ ++      L  +TPFD+G+G V+P+ ALDPGL+F   YEDY+ FLC
Sbjct: 635 TVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLC 694

Query: 312 SLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETY 371
           + PG+    +++ T   CN  +   HP++ N PSI +S LVG Q V R+V NV    ETY
Sbjct: 695 TTPGISAHEIRNYTNTACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETY 754

Query: 372 VCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             +        + + PP  T+     +   + +     +  ++FGE
Sbjct: 755 TITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFGE 793

BLAST of MELO3C021725 vs. Swiss-Prot
Match: SBT26_ARATH (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.3e-80
Identity = 158/379 (41.69%), Postives = 223/379 (58.84%), Query Frame = 1

Query: 38  ECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFI 97
           +CQ PE  +  +V+ +I+LC +S  F+ G++S+  +  TAK L   GFVL+         
Sbjct: 418 DCQKPEVLNKKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTK 477

Query: 98  AEPIPFRVPGILVPSVSDTQVILKYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAP 157
            +P+P  +PGIL+  VS +  ++ YY   T +D  G V++FK +  IG+G      K AP
Sbjct: 478 FDPVPSCIPGILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAP 537

Query: 158 TVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTS 217
            V+ FS+RGP+  + +   AD+LKPDILAPG  IW+AWS     E    G  FAL+SGTS
Sbjct: 538 EVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTS 597

Query: 218 MAAPHIVGVAALIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFN---LHALYPST 277
           MAAPHI G+AAL+KQK+P W+P+ I SA+ TT+T  D  G  +QA+ ++      L  +T
Sbjct: 598 MAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKAT 657

Query: 278 PFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVDPAVVKSATGGQCNASISHSHP 337
           PFD+G+G V+P+ ALDPGL+F   YEDYI FLC+ PG+D   +K+ T   CN  +   HP
Sbjct: 658 PFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKM--VHP 717

Query: 338 ADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQ 397
           ++ N PSI IS LV  Q V RRV NV  + ETY  +        + + PP  TV A   +
Sbjct: 718 SNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASR 777

Query: 398 NLEIQIIATHKTDHFTFGE 414
              + +     T  ++FG+
Sbjct: 778 TFSVTLTVRSVTGAYSFGQ 794

BLAST of MELO3C021725 vs. Swiss-Prot
Match: SBT23_ARATH (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 6.2e-79
Identity = 169/405 (41.73%), Postives = 239/405 (59.01%), Query Frame = 1

Query: 12  LHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLA 71
           ++K++ A  A + N T  D    Y+ ECQ+ E FD + V   +++CS+S  F+ G S++ 
Sbjct: 415 MYKMISAFHA-LNNSTSVDK-DMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIK 474

Query: 72  AIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDG 131
             +  AK L   G +   +P    F   P P  +PGI++PSV D++ +LKYY  +  +D 
Sbjct: 475 QALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDV 534

Query: 132 RGM-VREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQ 191
               +  F   A I  G  A+F   AP V  +S+RGPD  + + + ADVLKP+++APG+ 
Sbjct: 535 TTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNS 594

Query: 192 IWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTA 251
           IW AWS  S      +G +FA+MSGTSMAAPH+ GVAALIKQ YP +TPS I+SA+STTA
Sbjct: 595 IWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTA 654

Query: 252 TKYDMNGDLIQAEGFNLH---ALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLC 311
              D  G  I A+    +   +LY +TP D G+G V+ T ALDPGLVF T +EDYI+FLC
Sbjct: 655 LLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLC 714

Query: 312 SLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETY 371
            + G D  VV + TG +C A+ +     DLNLPSIT+S+L G Q  +R ++N+ G  ETY
Sbjct: 715 GINGSD-TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETY 774

Query: 372 VCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFG 413
                PP G ++ + P  F++A  E Q L + +  T  +   +FG
Sbjct: 775 NVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFG 815

BLAST of MELO3C021725 vs. Swiss-Prot
Match: SBT22_ARATH (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana GN=SBT2.2 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 8.1e-79
Identity = 165/408 (40.44%), Postives = 249/408 (61.03%), Query Frame = 1

Query: 13  HKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAA 72
           + ++ A DA ++N++   D   Y+ ECQ+  +FD ++++ ++++CS+S  F+ G S++  
Sbjct: 430 YTMISALDA-LKNKSSVVDKDMYVGECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQ 489

Query: 73  IIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDG- 132
            +  AK L   G V   +P    F   P P  +PGI++PS  D++V+LKYY  +  +DG 
Sbjct: 490 ALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGT 549

Query: 133 -RGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQ 192
            + +VR F   A I  G+ A+F   AP +  +S+RGPD  +   + AD+LKP+++APG+ 
Sbjct: 550 TKEIVR-FGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNS 609

Query: 193 IWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTA 252
           IW AWS  +      +G  FA+MSGTSMAAPH+ GVAAL+KQK+  ++PS IASA+STT+
Sbjct: 610 IWGAWSSAATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTS 669

Query: 253 TKYDMNGDLIQAEGFNLH---ALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLC 312
             +D  G+ I A+    +    + P+TPFD G G V+ T ALDPGL+F T +EDY++FLC
Sbjct: 670 VLFDNKGEAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLC 729

Query: 313 SLPGVDPAVVKSATGGQC---NASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKV 372
            + G  P VV + TG  C   NA+IS S   DLNLPSIT+S L   + V+R + N+ G  
Sbjct: 730 GINGSAP-VVFNYTGTNCLRNNATISGS---DLNLPSITVSKLNNTRTVQRLMTNIAGN- 789

Query: 373 ETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFG 413
           ETY  S+I P    +N+ P  F++A+ E + L + + A   +   +FG
Sbjct: 790 ETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFG 830

BLAST of MELO3C021725 vs. TrEMBL
Match: B9RXF5_RICCO (Subtilase, putative OS=Ricinus communis GN=RCOM_0903320 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 3.1e-149
Identity = 258/413 (62.47%), Postives = 324/413 (78.45%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGP+ G   F +KLV A+DA   N T +   P YIEECQ+PE+ DP +V+  IV+C+FS
Sbjct: 384 LSGPTFGYGLFKYKLVFAQDAVKANGT-FPRTPQYIEECQHPESLDPKLVRRRIVICTFS 443

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GF NGTSS+ AII T++ L+F GF L+ANP+YGDFIAEPIPF VPGI++P V+D ++I 
Sbjct: 444 AGFYNGTSSITAIIDTSRTLRFTGFALVANPSYGDFIAEPIPFAVPGIMIPKVADAEIIS 503

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           KYYE+   +D RG V +F  +  IGEGR+A+F   AP VSRFSSRGPD+++INR  ADVL
Sbjct: 504 KYYEQEILRDERGFVSKFCARGAIGEGRVAAFEGRAPIVSRFSSRGPDFLDINRIPADVL 563

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSPLSA +P+L G  FAL+SGTSMA PHIVG+AALIKQ +PSWTPS
Sbjct: 564 KPDILAPGHQIWAAWSPLSALDPILTGDNFALLSGTSMATPHIVGIAALIKQFHPSWTPS 623

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASA+STTA  YD  G+LI AEGF++++LYPST FD GAG V+PT A+DPGLVFP+E++
Sbjct: 624 MIASALSTTAGNYDNYGELILAEGFDINSLYPSTHFDLGAGFVNPTRAMDPGLVFPSEFQ 683

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           +YI+FLCSLPG+DPA+VK+ TG  CN S+  S PA+LNLPS+TIS+L G Q V R VKNV
Sbjct: 684 NYISFLCSLPGIDPAIVKATTGEPCNQSL--SSPANLNLPSVTISALRGSQTVERNVKNV 743

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
           G K ETY+ SVI PNGTTVN+ P WF +A +  QN++I+   TH  + F+FG+
Sbjct: 744 GIKPETYLSSVIAPNGTTVNLSPTWFIIAPQGTQNIDIEFHVTHARNEFSFGQ 793

BLAST of MELO3C021725 vs. TrEMBL
Match: W9RY25_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.0e-149
Identity = 258/421 (61.28%), Postives = 333/421 (79.10%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGPSLG+  FLHKLVLA+DA   N + + + PS IEECQ+P+A DP +V+ SIV+C+FS
Sbjct: 388 LSGPSLGNGLFLHKLVLARDAARTNGS-FPETPSLIEECQSPQALDPRVVRGSIVICTFS 447

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
           +GF NGTSS+ AII TAK L F GFV +ANP+YGDFIAEPIPF +PG+L+PSV+D QVIL
Sbjct: 448 EGFSNGTSSVTAIISTAKALGFAGFVFVANPSYGDFIAEPIPFDIPGLLIPSVADVQVIL 507

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
            YYE +T +    +VR F+ KA IGEGR+ASF  +APTVSRFSSRGPD+I+  R+  DVL
Sbjct: 508 NYYERHTHRGESKLVRRFRAKAAIGEGRVASFKSQAPTVSRFSSRGPDFIDAKRNPTDVL 567

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPG ++WAAWSP+SAS+P+L+G  FAL+SGTSMA PHI G+AALIKQ  PSWTPS
Sbjct: 568 KPDILAPGQRVWAAWSPISASDPILEGLSFALVSGTSMATPHIAGIAALIKQYNPSWTPS 627

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTE-- 300
           MI+SA+ TTATKYD +G +I AEGF++ +LYPSTPF+ G+GLV+P+ A+DPGLVF +E  
Sbjct: 628 MISSAIDTTATKYDNHGQVIMAEGFSIGSLYPSTPFELGSGLVTPSCAIDPGLVFSSESW 687

Query: 301 ------YEDYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQV 360
                 Y+DY+ FLCSLPG++PA +K A G  CN S+  +HPA+LNLPSITIS+L+G   
Sbjct: 688 GVDEAGYKDYVAFLCSLPGMNPATIKIAIGESCNYSL--THPANLNLPSITISALIGSLS 747

Query: 361 VRRRVKNVGGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFG 414
           V+R V+NV  + ETY+C+V+PPNGTTV++ PPWFT+A +  Q L++Q+  T   + F+FG
Sbjct: 748 VQRTVRNVRNEPETYLCAVLPPNGTTVSLNPPWFTIAPQGTQELDVQLNVTKAMNDFSFG 805

BLAST of MELO3C021725 vs. TrEMBL
Match: A0A067L7P5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02112 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.7e-147
Identity = 266/448 (59.38%), Postives = 331/448 (73.88%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +S P+ G   FL+KLVLA+DA   N T +   P YIEECQ PE+ DP++V++SIV+C+FS
Sbjct: 392 LSAPTFGDGRFLYKLVLAQDAVKANGT-FPRTPQYIEECQYPESLDPSVVRDSIVICTFS 451

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GF NGTS++ AII T + L FMGF L+ANP YGDFIAEPIPF V GI++ +V+D Q+IL
Sbjct: 452 TGFYNGTSTINAIIDTTRTLGFMGFALVANPIYGDFIAEPIPFAVSGIMISNVADAQIIL 511

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYE+   +D RG V EF  +A IGEGR+ASF   AP VSRFSSRGPD+I+INR+ ADVL
Sbjct: 512 QYYEQQIKRDERGFVTEFHARAAIGEGRVASFEGRAPIVSRFSSRGPDFIDINRTPADVL 571

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSPLSA EP+L GY+FAL+SGTSMA PHI G+AA IKQ  P WTPS
Sbjct: 572 KPDILAPGHQIWAAWSPLSALEPMLTGYKFALLSGTSMATPHIGGIAAFIKQMNPFWTPS 631

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MI+SAMSTTATKYD  G  I AEGF++++ YPST FD GAGLV+PT ++DPGLVF +E+E
Sbjct: 632 MISSAMSTTATKYDNYGQPILAEGFDINSFYPSTHFDLGAGLVNPTRSVDPGLVFTSEFE 691

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYI+FLCSLP ++   VK ATG  CN     SHPA+LNLPS+TIS+L G Q V+R VKNV
Sbjct: 692 DYISFLCSLPNINRTTVKVATGESCNQLF--SHPANLNLPSVTISALKGSQTVQRSVKNV 751

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGENFFDRKF 420
           G K ETY+ SV+ PNGTTV + P WFT+A +  Q++EIQ + T   D F+FGE  F    
Sbjct: 752 GSKPETYLGSVMSPNGTTVTLSPTWFTIAPQGTQHIEIQFLVTKAGDEFSFGEIVFTGSL 811

Query: 421 KPYCKNPFINFGCFCILRVRIGLFPLKV 449
                          I+R+ + +FP+ +
Sbjct: 812 NH-------------IVRIPLSVFPVSI 823

BLAST of MELO3C021725 vs. TrEMBL
Match: A0A061GNV8_THECC (PA-domain containing subtilase family protein OS=Theobroma cacao GN=TCM_030299 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.0e-144
Identity = 253/413 (61.26%), Postives = 323/413 (78.21%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           ++ PSLG+   L+KLVLAKDA +  +  +   P Y+EECQ PEA DPNIV+ SIV+C+FS
Sbjct: 390 LTAPSLGNGSVLYKLVLAKDA-VNLKGAFPRTPPYVEECQYPEALDPNIVRGSIVICTFS 449

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GF N TS+L AII TA+ L FMGFVL+ANP+YGDFIA+PIP+ V G L+P V+D +++ 
Sbjct: 450 AGFSNETSTLTAIIDTARTLGFMGFVLVANPSYGDFIAQPIPYSVSGTLIPKVADAKIVS 509

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYE+ T +D  G VREF  +A IG+GR+ASF  +AP VSRFSSRGPD+I+IN+  ADVL
Sbjct: 510 QYYEQQTYRDAGGFVREFNARAAIGDGRVASFCGQAPIVSRFSSRGPDFIDINKDPADVL 569

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGH+IWAAWSP+SA +P+L GY FAL+SGTSMAAPH+ G+AALIKQKYPSWTPS
Sbjct: 570 KPDILAPGHEIWAAWSPMSALDPILSGYNFALLSGTSMAAPHVAGIAALIKQKYPSWTPS 629

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASA+STTA+K+D NG+LI+AEG ++ +LY ST FD GAG VSP+ A+DPGLV  +E+E
Sbjct: 630 MIASAISTTASKFDNNGELIRAEGSDVGSLYSSTYFDSGAGFVSPSRAMDPGLVLSSEFE 689

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYI+FLCS+P +D   +++ATG  C+ S+    PA+LNLPS+TIS+L     VRR  KNV
Sbjct: 690 DYISFLCSIPNIDRFAIRAATGVWCSRSL--GLPANLNLPSVTISALERSVTVRRSFKNV 749

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             K ETYV   IPPNGTT+ + PPWF +A EE Q+L+I+I A   T+ F+FGE
Sbjct: 750 ATKPETYVSLAIPPNGTTITLNPPWFAIAPEETQDLDIEINAIQSTNQFSFGE 799

BLAST of MELO3C021725 vs. TrEMBL
Match: A0A068U8B7_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017837001 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.0e-144
Identity = 255/413 (61.74%), Postives = 310/413 (75.06%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGPS G     H+L+LAKDA ++N   +  I  YIEECQ PEA DP +V  S+V+C+FS
Sbjct: 394 LSGPSSGQGLLQHRLILAKDAVIRNGN-FPRISQYIEECQYPEALDPAVVLGSVVICTFS 453

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GF NGTS+L AII TAK L F+GFVL+ANP YGDFIAEPIPF VPGI++P  SD  +I 
Sbjct: 454 DGFYNGTSNLTAIIKTAKVLGFVGFVLVANPRYGDFIAEPIPFSVPGIMIPRTSDALLIS 513

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYEE T +D RG+V  + G+A IGEGR A+    APTVSRFS+RGPDYI+  ++  DVL
Sbjct: 514 QYYEEQTSRDKRGLVISYSGRAAIGEGRTAACMGRAPTVSRFSARGPDYIDQRKNPTDVL 573

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSPLSAS+P+L G  FAL+SGTSMA PHI G+AALIKQ  P WTPS
Sbjct: 574 KPDILAPGHQIWAAWSPLSASDPMLAGQNFALISGTSMATPHISGIAALIKQNNPKWTPS 633

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASAMSTTATKYD  GD I A GF + +LY + PF FGAG V+P+ A+DPGLVF   YE
Sbjct: 634 MIASAMSTTATKYDNQGDQIMAHGFEIDSLYTAAPFGFGAGHVNPSRAMDPGLVFSAGYE 693

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYI+FLCSLP +DPA +++ATGG C +S     PADLNL S+TI++L G + V RRV NV
Sbjct: 694 DYISFLCSLPNLDPASIRTATGGPCTSSF--GSPADLNLASVTITALSGSRTVHRRVTNV 753

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             K ETYVC V+PP G  ++I PPWF VA + +Q+LEI++  T   D F+FGE
Sbjct: 754 ASKPETYVCGVLPPVGVMIDIHPPWFRVAPQGIQDLEIRLNVTQALDDFSFGE 803

BLAST of MELO3C021725 vs. TAIR10
Match: AT1G62340.1 (AT1G62340.1 PA-domain containing subtilase family protein)

HSP 1 Score: 474.6 bits (1220), Expect = 7.2e-134
Identity = 235/413 (56.90%), Postives = 295/413 (71.43%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGP+LG+    H+LVLAKDA   N +    +   IEECQ PE FDP  V  SIV+C+FS
Sbjct: 402 LSGPTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSIVICTFS 461

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GF N  S++ AI  TA+ L FMGF+LIANP +GD++AEP+ F  PGIL+P+VS  Q+IL
Sbjct: 462 DGFYNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIIL 521

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYEE T +D RG+  +F  +A IGEGR + F  +AP VSRFSSRGP +I+  RS  DVL
Sbjct: 522 RYYEEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVL 581

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIW AWS  SA +P+L G  FA++SGTSMA PHI G+ ALIKQ  PSWTP+
Sbjct: 582 KPDILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPA 641

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASA+STTA +YD NG++I AE + L  L+PS  FD GAG V+P  ALDPGLV P  +E
Sbjct: 642 MIASAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFE 701

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYI+FLCSLP + PA ++ ATG  C  ++  SHPA+LN PS+TIS+L    VVRR  ++V
Sbjct: 702 DYISFLCSLPNISPATIRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDV 761

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             K ETY+ SV+PPNGTTV + P WFTV  ++ Q+L+I+   T   + FTFGE
Sbjct: 762 SNKTETYLGSVLPPNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGE 812

BLAST of MELO3C021725 vs. TAIR10
Match: AT2G19170.1 (AT2G19170.1 subtilisin-like serine protease 3)

HSP 1 Score: 311.2 bits (796), Expect = 1.1e-84
Identity = 165/406 (40.64%), Postives = 241/406 (59.36%), Query Frame = 1

Query: 12  LHKLVLAKDAKMQNE-TQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSL 71
           L+ LV A D  + +  ++Y+  PS   +CQ PE F+  +V+ +I+LC +S  F+ GT+S+
Sbjct: 395 LYTLVSANDVLLDSSVSKYN--PS---DCQRPEVFNKKLVEGNILLCGYSFNFVVGTASI 454

Query: 72  AAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKD 131
             ++ TAK L   GFVL+          +P+P  +PGIL+  VS +  ++ YY  +T +D
Sbjct: 455 KKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDVSKSMDLIDYYNASTSRD 514

Query: 132 GRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQ 191
             G V+ FK +  IG+G      K AP V+ FS+RGP+  + +   AD+LKPDILAPG+ 
Sbjct: 515 WTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYL 574

Query: 192 IWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTA 251
           IWAAW P    EP   G  FAL+SGTSMAAPHI G+AAL+KQK+P W+P+ I SA+ TT+
Sbjct: 575 IWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTS 634

Query: 252 TKYDMNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLC 311
           T  D  G L+QA+ ++      L  +TPFD+G+G V+P+ ALDPGL+F   YEDY+ FLC
Sbjct: 635 TVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLC 694

Query: 312 SLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETY 371
           + PG+    +++ T   CN  +   HP++ N PSI +S LVG Q V R+V NV    ETY
Sbjct: 695 TTPGISAHEIRNYTNTACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETY 754

Query: 372 VCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             +        + + PP  T+     +   + +     +  ++FGE
Sbjct: 755 TITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFGE 793

BLAST of MELO3C021725 vs. TAIR10
Match: AT4G30020.1 (AT4G30020.1 PA-domain containing subtilase family protein)

HSP 1 Score: 300.1 bits (767), Expect = 2.4e-81
Identity = 158/379 (41.69%), Postives = 223/379 (58.84%), Query Frame = 1

Query: 38  ECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFI 97
           +CQ PE  +  +V+ +I+LC +S  F+ G++S+  +  TAK L   GFVL+         
Sbjct: 418 DCQKPEVLNKKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTK 477

Query: 98  AEPIPFRVPGILVPSVSDTQVILKYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAP 157
            +P+P  +PGIL+  VS +  ++ YY   T +D  G V++FK +  IG+G      K AP
Sbjct: 478 FDPVPSCIPGILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAP 537

Query: 158 TVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTS 217
            V+ FS+RGP+  + +   AD+LKPDILAPG  IW+AWS     E    G  FAL+SGTS
Sbjct: 538 EVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTS 597

Query: 218 MAAPHIVGVAALIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFN---LHALYPST 277
           MAAPHI G+AAL+KQK+P W+P+ I SA+ TT+T  D  G  +QA+ ++      L  +T
Sbjct: 598 MAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKAT 657

Query: 278 PFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVDPAVVKSATGGQCNASISHSHP 337
           PFD+G+G V+P+ ALDPGL+F   YEDYI FLC+ PG+D   +K+ T   CN  +   HP
Sbjct: 658 PFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKM--VHP 717

Query: 338 ADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQ 397
           ++ N PSI IS LV  Q V RRV NV  + ETY  +        + + PP  TV A   +
Sbjct: 718 SNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASR 777

Query: 398 NLEIQIIATHKTDHFTFGE 414
              + +     T  ++FG+
Sbjct: 778 TFSVTLTVRSVTGAYSFGQ 794

BLAST of MELO3C021725 vs. TAIR10
Match: AT5G44530.1 (AT5G44530.1 Subtilase family protein)

HSP 1 Score: 296.2 bits (757), Expect = 3.5e-80
Identity = 169/405 (41.73%), Postives = 239/405 (59.01%), Query Frame = 1

Query: 12  LHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLA 71
           ++K++ A  A + N T  D    Y+ ECQ+ E FD + V   +++CS+S  F+ G S++ 
Sbjct: 415 MYKMISAFHA-LNNSTSVDK-DMYVGECQDYENFDQDRVSGKLLICSYSARFVLGLSTIK 474

Query: 72  AIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDG 131
             +  AK L   G +   +P    F   P P  +PGI++PSV D++ +LKYY  +  +D 
Sbjct: 475 QALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYNSSIQRDV 534

Query: 132 RGM-VREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQ 191
               +  F   A I  G  A+F   AP V  +S+RGPD  + + + ADVLKP+++APG+ 
Sbjct: 535 TTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNS 594

Query: 192 IWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTA 251
           IW AWS  S      +G +FA+MSGTSMAAPH+ GVAALIKQ YP +TPS I+SA+STTA
Sbjct: 595 IWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTA 654

Query: 252 TKYDMNGDLIQAEGFNLH---ALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLC 311
              D  G  I A+    +   +LY +TP D G+G V+ T ALDPGLVF T +EDYI+FLC
Sbjct: 655 LLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLC 714

Query: 312 SLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETY 371
            + G D  VV + TG +C A+ +     DLNLPSIT+S+L G Q  +R ++N+ G  ETY
Sbjct: 715 GINGSD-TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETY 774

Query: 372 VCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFG 413
                PP G ++ + P  F++A  E Q L + +  T  +   +FG
Sbjct: 775 NVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFG 815

BLAST of MELO3C021725 vs. TAIR10
Match: AT4G20430.1 (AT4G20430.1 Subtilase family protein)

HSP 1 Score: 295.8 bits (756), Expect = 4.6e-80
Identity = 165/408 (40.44%), Postives = 249/408 (61.03%), Query Frame = 1

Query: 13  HKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAA 72
           + ++ A DA ++N++   D   Y+ ECQ+  +FD ++++ ++++CS+S  F+ G S++  
Sbjct: 430 YTMISALDA-LKNKSSVVDKDMYVGECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQ 489

Query: 73  IIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDG- 132
            +  AK L   G V   +P    F   P P  +PGI++PS  D++V+LKYY  +  +DG 
Sbjct: 490 ALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGT 549

Query: 133 -RGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQ 192
            + +VR F   A I  G+ A+F   AP +  +S+RGPD  +   + AD+LKP+++APG+ 
Sbjct: 550 TKEIVR-FGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNS 609

Query: 193 IWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTA 252
           IW AWS  +      +G  FA+MSGTSMAAPH+ GVAAL+KQK+  ++PS IASA+STT+
Sbjct: 610 IWGAWSSAATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTS 669

Query: 253 TKYDMNGDLIQAEGFNLH---ALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLC 312
             +D  G+ I A+    +    + P+TPFD G G V+ T ALDPGL+F T +EDY++FLC
Sbjct: 670 VLFDNKGEAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLC 729

Query: 313 SLPGVDPAVVKSATGGQC---NASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKV 372
            + G  P VV + TG  C   NA+IS S   DLNLPSIT+S L   + V+R + N+ G  
Sbjct: 730 GINGSAP-VVFNYTGTNCLRNNATISGS---DLNLPSITVSKLNNTRTVQRLMTNIAGN- 789

Query: 373 ETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFG 413
           ETY  S+I P    +N+ P  F++A+ E + L + + A   +   +FG
Sbjct: 790 ETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFG 830

BLAST of MELO3C021725 vs. NCBI nr
Match: gi|659118464|ref|XP_008459133.1| (PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo])

HSP 1 Score: 838.2 bits (2164), Expect = 7.0e-240
Identity = 414/415 (99.76%), Postives = 414/415 (99.76%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS
Sbjct: 361 MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 420

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
           QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL
Sbjct: 421 QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 480

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL
Sbjct: 481 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 540

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS
Sbjct: 541 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 600

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE
Sbjct: 601 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 660

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV
Sbjct: 661 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 720

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGENF 416
           GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE F
Sbjct: 721 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIF 775

BLAST of MELO3C021725 vs. NCBI nr
Match: gi|778665448|ref|XP_004145469.2| (PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus])

HSP 1 Score: 798.5 bits (2061), Expect = 6.2e-228
Identity = 396/413 (95.88%), Postives = 403/413 (97.58%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           MSGPSLGSEFFLHKLVLAKDA  QNET Y+DIPSYIEECQ+PEAFDPNIVQNSIVLCSFS
Sbjct: 330 MSGPSLGSEFFLHKLVLAKDATKQNETNYNDIPSYIEECQHPEAFDPNIVQNSIVLCSFS 389

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
           QGFLNGTSSLAAIIHTAK+LKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL
Sbjct: 390 QGFLNGTSSLAAIIHTAKQLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 449

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           KYYEENTCKD RGMVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVL
Sbjct: 450 KYYEENTCKDERGMVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVL 509

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSPLSASEPLLKGY FALMSGTSMAAPHIVG+AALIKQKYPSWTPS
Sbjct: 510 KPDILAPGHQIWAAWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPS 569

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYE
Sbjct: 570 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYE 629

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           D INFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNV
Sbjct: 630 DNINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNV 689

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
           GGKVETYV SVIPPNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGE
Sbjct: 690 GGKVETYVWSVIPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGE 742

BLAST of MELO3C021725 vs. NCBI nr
Match: gi|1009108180|ref|XP_015883427.1| (PREDICTED: subtilisin-like protease SBT2.4 [Ziziphus jujuba])

HSP 1 Score: 560.1 bits (1442), Expect = 3.7e-156
Identity = 266/413 (64.41%), Postives = 334/413 (80.87%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGPS G   FL+KLVLA+DA  +N T +   P YIEECQ PEA DPN+V++SIV+C FS
Sbjct: 383 LSGPSFGGGLFLYKLVLARDAVKRNGT-FPRTPPYIEECQYPEALDPNVVKHSIVICIFS 442

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
            GFLNG+S+L AII TA+ L FMGFVL+ANPN+GDFIAEP+PF + GIL+P+V+  QVI 
Sbjct: 443 AGFLNGSSTLTAIIDTAETLGFMGFVLVANPNFGDFIAEPLPFDISGILIPTVAGAQVIS 502

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
            YYE+ T +DG+G+V  F  +A IGEGRIA F  + PTVSRFSSRGPD+I++ R+ ADVL
Sbjct: 503 NYYEQKTYRDGKGLVTRFTARAAIGEGRIACFKDQVPTVSRFSSRGPDFIDVKRNPADVL 562

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQ+WAAWSP+SA EP+L GY F L+SGTSMA PH+ G+AAL+KQ  PSWTPS
Sbjct: 563 KPDILAPGHQVWAAWSPISALEPILMGYNFGLLSGTSMATPHVAGIAALLKQYNPSWTPS 622

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASAM+TTA+KYD +GDLI+AEG ++ +LYPSTPFDFG+GL+SP +AL PGLVF +EYE
Sbjct: 623 MIASAMATTASKYDNDGDLIKAEGSSIGSLYPSTPFDFGSGLLSPKSALHPGLVFSSEYE 682

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DY+ FLCSLP ++PA++++ATG  CN S+   HPA+LN+PSITISSL+G Q+V+R VKNV
Sbjct: 683 DYVGFLCSLPDIEPAMIRNATGEWCNYSL--GHPANLNVPSITISSLIGSQLVQRTVKNV 742

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
             K ETY+CSV+PPNGT VN+ PPWFT+A +E Q+L +Q   T     F+FGE
Sbjct: 743 ESKAETYLCSVLPPNGTVVNLYPPWFTIAPQETQDLYMQFNVTEAIGEFSFGE 792

BLAST of MELO3C021725 vs. NCBI nr
Match: gi|657984334|ref|XP_008384255.1| (PREDICTED: subtilisin-like protease [Malus domestica])

HSP 1 Score: 540.4 bits (1391), Expect = 3.0e-150
Identity = 265/413 (64.16%), Postives = 320/413 (77.48%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGP+ G    LHKLVLAKDA   + T +   P Y+EECQ PEA D N+V  SIV+C+FS
Sbjct: 392 LSGPTFGYGLLLHKLVLAKDAVKADGT-FPRTPPYVEECQYPEALDHNVVLGSIVICTFS 451

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
           +GF NGTS+L AII+TAK L FMGF+ +ANP YGDFIAEPIPF VPGILVP+++++QVIL
Sbjct: 452 EGFYNGTSTLTAIINTAKGLGFMGFIFVANPAYGDFIAEPIPFVVPGILVPNLTNSQVIL 511

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYE+ T K+ +G    F  +A IGEGR+ASF  +AP VSRFSSRGPD++N +R+  DVL
Sbjct: 512 QYYEQETHKEEKGFATRFAAEAAIGEGRVASFMGQAPVVSRFSSRGPDFVNTSRTPCDVL 571

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSP+SASEP L GY FAL SGTSMA PH+ G+AAL+KQ  PSWTPS
Sbjct: 572 KPDILAPGHQIWAAWSPVSASEPALTGYNFALQSGTSMATPHVAGIAALVKQYNPSWTPS 631

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASA+STTATKYD NG+LI AEG  + + YPS+ FDFGAGLVSP+ ALDPGLV PT YE
Sbjct: 632 MIASAISTTATKYDKNGELIMAEGSGIGSTYPSSHFDFGAGLVSPSRALDPGLVLPTGYE 691

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYI+FLCSLP + PA V++ATG  CN ++   HPA+LNLPSIT+S+L G Q+ RR   NV
Sbjct: 692 DYISFLCSLPEISPAAVRNATGEPCNNTL--DHPANLNLPSITLSALRGSQIXRRIFNNV 751

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
           G K ETYVCS + PNGT V++ P WF +A E +Q L IQ+  T   D FTFGE
Sbjct: 752 GSKPETYVCSAVSPNGTIVDLCPTWFRIAPEGIQELLIQVKVTQAMDDFTFGE 801

BLAST of MELO3C021725 vs. NCBI nr
Match: gi|694351639|ref|XP_009357595.1| (PREDICTED: subtilisin-like protease [Pyrus x bretschneideri])

HSP 1 Score: 538.9 bits (1387), Expect = 8.8e-150
Identity = 266/413 (64.41%), Postives = 320/413 (77.48%), Query Frame = 1

Query: 1   MSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPNIVQNSIVLCSFS 60
           +SGP+ G    LHKLVLAKDA   + T +   P Y+EECQ PEA D N+V  SIV+C+FS
Sbjct: 370 LSGPTFGYGLLLHKLVLAKDAVKADGT-FPRTPPYVEECQYPEALDHNVVLGSIVICTFS 429

Query: 61  QGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGILVPSVSDTQVIL 120
           +GF NGTS+L AII+TAK L F+GF+L+A+P YGDFIAEPIPF VPGILVP+++++QVIL
Sbjct: 430 EGFYNGTSTLTAIINTAKGLGFVGFILVASPVYGDFIAEPIPFVVPGILVPNLTNSQVIL 489

Query: 121 KYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVL 180
           +YYE  T K+ +G    F  KA IGEGR+ASF  +AP VSRFSSRGPD++N +R+  DVL
Sbjct: 490 QYYERETHKEEKGFATRFAAKAAIGEGRVASFMGQAPVVSRFSSRGPDFVNTSRTPCDVL 549

Query: 181 KPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPS 240
           KPDILAPGHQIWAAWSP+SASEP L GY FAL SGTSMA PH+ G+AAL+KQ  PSWTPS
Sbjct: 550 KPDILAPGHQIWAAWSPVSASEPTLTGYSFALQSGTSMATPHVAGIAALVKQYNPSWTPS 609

Query: 241 MIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYE 300
           MIASA+STTATKYD NG+LI AEG  + + YPS+ FDFGAGLVSP+ ALDPGLV P+ YE
Sbjct: 610 MIASAISTTATKYDKNGELIMAEGSGIGSRYPSSHFDFGAGLVSPSRALDPGLVLPSGYE 669

Query: 301 DYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNV 360
           DYINFLCSLP + PA V++ATG  CN ++   HPA+LNLPSITIS+L G Q+VRR   NV
Sbjct: 670 DYINFLCSLPEISPAAVRNATGEPCNNTL--DHPANLNLPSITISALRGSQIVRRIFNNV 729

Query: 361 GGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGE 414
           G K ETYVCS + PNGT V++ P WF +A E +Q L IQ+  T     FTFGE
Sbjct: 730 GSKPETYVCSAVSPNGTIVDLCPTWFRIAPEGIQELHIQVKVTQAMYDFTFGE 779

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT24_ARATH1.3e-13256.90Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1[more]
SBT25_ARATH1.9e-8340.64Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1[more]
SBT26_ARATH4.3e-8041.69Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1[more]
SBT23_ARATH6.2e-7941.73Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1[more]
SBT22_ARATH8.1e-7940.44Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana GN=SBT2.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
B9RXF5_RICCO3.1e-14962.47Subtilase, putative OS=Ricinus communis GN=RCOM_0903320 PE=3 SV=1[more]
W9RY25_9ROSA4.0e-14961.28Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1[more]
A0A067L7P5_JATCU1.7e-14759.38Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02112 PE=4 SV=1[more]
A0A061GNV8_THECC1.0e-14461.26PA-domain containing subtilase family protein OS=Theobroma cacao GN=TCM_030299 P... [more]
A0A068U8B7_COFCA5.0e-14461.74Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00017837001 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G62340.17.2e-13456.90 PA-domain containing subtilase family protein[more]
AT2G19170.11.1e-8440.64 subtilisin-like serine protease 3[more]
AT4G30020.12.4e-8141.69 PA-domain containing subtilase family protein[more]
AT5G44530.13.5e-8041.73 Subtilase family protein[more]
AT4G20430.14.6e-8040.44 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|659118464|ref|XP_008459133.1|7.0e-24099.76PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo][more]
gi|778665448|ref|XP_004145469.2|6.2e-22895.88PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus][more]
gi|1009108180|ref|XP_015883427.1|3.7e-15664.41PREDICTED: subtilisin-like protease SBT2.4 [Ziziphus jujuba][more]
gi|657984334|ref|XP_008384255.1|3.0e-15064.16PREDICTED: subtilisin-like protease [Malus domestica][more]
gi|694351639|ref|XP_009357595.1|8.8e-15064.41PREDICTED: subtilisin-like protease [Pyrus x bretschneideri][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0042335 cuticle development
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C021725T1MELO3C021725T1mRNA


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 155..303
score: 5.5E-32coord: 56..114
score: 5.5
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 139..253
score: 4.0
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 159..293
score: 4.45
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 13..123
score: 2.0E-179coord: 140..413
score: 2.0E
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 215..225
scor
NoneNo IPR availablePANTHERPTHR10795:SF390SUBTILISIN-LIKE SERINE PROTEASEcoord: 13..123
score: 2.0E-179coord: 140..413
score: 2.0E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
MELO3C021725Csa1G701250Cucumber (Chinese Long) v2cumeB017
MELO3C021725CSPI01G34240Wild cucumber (PI 183967)cpimeB013
MELO3C021725Cucsa.332670Cucumber (Gy14) v1cgymeB543
MELO3C021725CmoCh18G005460Cucurbita moschata (Rifu)cmomeB357
The following gene(s) are paralogous to this gene:

None