CSPI01G34240 (gene) Wild cucumber (PI 183967)

NameCSPI01G34240
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionSubtilisin-like protease
LocationChr1 : 29123072 .. 29125161 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAGAGAATTTAAAGGGAAAGCAGGGATTGGAGAAGGAAGAATTGCTTCATTTGGGAACCAAGCACCCACAGTTAGCAGATTTTCATCTAGAGGGCCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCAGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTAAAGGGTCAGCTTTTCATGAACTTATTGTTCTTTCAGTTTCATCAATTTTTCCCTTTTACATTTGTCATTCAATCTATATGAATTTTAAGCAATTCTTGTTTTCGTCTCTTTAGGTTTTAAAACCCCAGTGCTAAAAAAAATATATTTGTTTTTAGTTTTCTCTGTATGAAATTTATATGTACTGTTTTTCTCTTAAATTTTATATTATGGTTCTTACATTTATAAAACAAACAATTGGGTTCTCAGTAAACTTTTACAATTACATGACCCTACTCTTTCTTAATTTCTTATGATCTTTTGCATCTCTCTCAAGTAATATCTAGTTGAACTTCCTAGCCAAATTCTATAAACAAGAAAAATTTCTTAAAAAGCTAATCTTTCTAGTTTTCAGAACTTTAATTTGAAACCTGGTTTTTGAAAACAACATATAGATAACAAAATTACAAAAAGCTTAGAGGTGGAAGAAGTATCTACCGGCATGCTTTTAATTCCTAAAAGTTAAAAATTGAAAACAAAGTTATCAAATAAATTTTTTTTTCTAGTCTTTTAAATTTTGTTTTGACTTTTACAAATATCAATGAAAAGTGAATAACAAAACATAAACAAAGAAACATAGTGAAACTAGAGTTTATTAGTTTAATTTTTTAAAAAAAAAAAAAATTAGTAAAGACGATCTAGATAAATTTCACCTTTATAAGTCCCAATCAACTCAAGAAATTAGAAAGTCATATTTATGTCATATCAAAAGGGCACAAGAGAAGTTGATTGAAACATGAGTGTTGAATTATATATATACAGGATACCATTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAATAGCAGCACTTATCAAGCAAAAATATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTCGATTTCGGCGCTGGGCTCGTTTCTCCGTCCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGGTAAATTCTAATGATACATAACTCTCTACCTTTTCATTATTTGGACAACTGTGTGTTTGTTGAATATCTCAAAGCTACTTAATAACGCTTTGAAATGTTTGTGGTAGTAAAAAAGAACTCACCAATTAATGTTCCCTTGAAGTCTAACCGTGCTTGTGTTAGGAATACCACTTAACATGAAAGCTTAGAGGAATACAAAGAAACATCAAGATAGTAATATATTACAATATTAAAGAAAAAACTAAACTAATATGGCTGATATCTAAGAATTGTAATTTAACAACTCTAAACTAGAAATACAAATATTGCAAATTTCTTTTGCCAACAGATTCAGTCTAAGCCTATTCAAACATCAAGAGAACTAACCATATCTTCTTGAAACAGAATATGAAGACAATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTCGTCAAAAGCGCCACCGGAGGACAATGTAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGGTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAATCCGCCGGTGTTCACTGTAGCTGCCGAAGAAGTCCAAAACTTGGAAATACAGATCATTGCCACACATAAAACGGATCATTTTACCTTTGGGGAGATTATTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATCCTGA

mRNA sequence

ATGGTTAGAGAATTTAAAGGGAAAGCAGGGATTGGAGAAGGAAGAATTGCTTCATTTGGGAACCAAGCACCCACAGTTAGCAGATTTTCATCTAGAGGGCCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCAGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTAAAGGGATACCATTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAATAGCAGCACTTATCAAGCAAAAATATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTCGATTTCGGCGCTGGGCTCGTTTCTCCGTCCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGAATATGAAGACAATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTCGTCAAAAGCGCCACCGGAGGACAATGTAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGGTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAATCCGCCGGTGTTCACTGTAGCTGCCGAAGAAGTCCAAAACTTGGAAATACAGATCATTGCCACACATAAAACGGATCATTTTACCTTTGGGGAGATTATTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATCCTGA

Coding sequence (CDS)

ATGGTTAGAGAATTTAAAGGGAAAGCAGGGATTGGAGAAGGAAGAATTGCTTCATTTGGGAACCAAGCACCCACAGTTAGCAGATTTTCATCTAGAGGGCCAGATTACATAAACATAAATAGGAGTTTAGCTGATGTTCTAAAGCCAGATATTCTTGCACCTGGTCACCAAATTTGGGCTGCTTGGAGCCCTCTCAGTGCCTCAGAACCACTATTAAAGGGATACCATTTTGCACTGATGTCTGGAACAAGTATGGCAGCACCTCATATTGTGGGAATAGCAGCACTTATCAAGCAAAAATATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCTATGTCCACAACTGCTACAAAGTATGATATGAATGGAGACCTTATTCAGGCTGAGGGATTCAACCTTCATGCTTTATATCCTTCTACTCCCTTCGATTTCGGCGCTGGGCTCGTTTCTCCGTCCAACGCTTTGGACCCCGGACTCGTCTTCCCAACAGAATATGAAGACAATATAAACTTCTTGTGCTCTTTACCTGGGGTTGATCCTGCCGTCGTCAAAAGCGCCACCGGAGGACAATGTAATGCCTCTATCTCACACTCACACCCTGCAGATCTGAACCTTCCTTCAATCACAATATCATCACTGGTTGGGCATCAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGAAAAGTGGAGACATATGTATGGTCAGTGATCCCACCAAACGGGACAACAGTCAACATCAATCCGCCGGTGTTCACTGTAGCTGCCGAAGAAGTCCAAAACTTGGAAATACAGATCATTGCCACACATAAAACGGATCATTTTACCTTTGGGGAGATTATTTTGACAGGAAGTTTAAACCATATTGCAAGAATCCCTTTATCAATTTTGGTTGTTTCTGCATCCTGA
BLAST of CSPI01G34240 vs. Swiss-Prot
Match: SBT24_ARATH (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-94
Identity = 173/297 (58.25%), Postives = 218/297 (73.40%), Query Frame = 1

Query: 4   EFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWS 63
           +F  +A IGEGR + F  +AP VSRFSSRGP +I+  RS  DVLKPDILAPGHQIW AWS
Sbjct: 538 QFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWGAWS 597

Query: 64  PLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMN 123
             SA +P+L G  FA++SGTSMA PHI GI ALIKQ  PSWTP+MIASA+STTA +YD N
Sbjct: 598 LPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEYDSN 657

Query: 124 GDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDPAV 183
           G++I AE + L  L+PS  FD GAG V+P+ ALDPGLV P  +ED I+FLCSLP + PA 
Sbjct: 658 GEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNISPAT 717

Query: 184 VKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPPNG 243
           ++ ATG  C  ++  SHPA+LN PS+TIS+L    VV+R  ++V  K ETY+ SV+PPNG
Sbjct: 718 IRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLPPNG 777

Query: 244 TTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 301
           TTV + P  FTV  ++ Q+L+I+   T   + FTFGE++LTGSLNHI RIPLS+  +
Sbjct: 778 TTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI 832

BLAST of CSPI01G34240 vs. Swiss-Prot
Match: SBT25_ARATH (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-65
Identity = 131/300 (43.67%), Postives = 180/300 (60.00%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V+ FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ IWAA
Sbjct: 514 VKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAA 573

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           W P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 574 WCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 633

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G L+QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED + FLC+ PG
Sbjct: 634 RAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPG 693

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +    +++ T   CN  +   HP++ N PSI +S LVG Q V R+V NV    ETY  + 
Sbjct: 694 ISAHEIRNYTNTACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETYTITA 753

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 299
                  + +NPP  T+     +   + +     +  ++FGE+ L GS  H  RIP+  L
Sbjct: 754 RMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFGEVKLKGSRGHKVRIPVVAL 811

BLAST of CSPI01G34240 vs. Swiss-Prot
Match: SBT23_ARATH (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.8e-63
Identity = 137/302 (45.36%), Postives = 189/302 (62.58%), Query Frame = 1

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G  A+F N+AP V  +S+RGPD  + + + ADVLKP+++APG+ IW AWS 
Sbjct: 540 FGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSS 599

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            S      +G  FA+MSGTSMAAPH+ G+AALIKQ YP +TPS I+SA+STTA   D  G
Sbjct: 600 ASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKG 659

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
             I A+    +   +LY +TP D G+G V+ + ALDPGLVF T +ED I+FLC + G D 
Sbjct: 660 SPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGINGSD- 719

Query: 185 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 244
            VV + TG +C A+ +     DLNLPSIT+S+L G Q  QR ++N+ G  ETY     PP
Sbjct: 720 TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETYNVGWSPP 779

Query: 245 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 304
            G ++ ++P  F++A  E Q L + +  T  +   +FG I L G+  HI  IP++++   
Sbjct: 780 YGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTVIAKI 839

BLAST of CSPI01G34240 vs. Swiss-Prot
Match: SBT26_ARATH (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.4e-63
Identity = 130/297 (43.77%), Postives = 178/297 (59.93%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V++FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  IW+A
Sbjct: 515 VKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSA 574

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WS     E    G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 575 WSANGTDEANYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 634

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G  +QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED I FLC+ PG
Sbjct: 635 RAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPG 694

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +D   +K+ T   CN  +   HP++ N PSI IS LV  Q V RRV NV  + ETY  + 
Sbjct: 695 IDAHEIKNFTNTPCNFKM--VHPSNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITS 754

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPL 296
                  + ++PP  TV A   +   + +     T  ++FG++ L GS  H   +P+
Sbjct: 755 RMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVTGAYSFGQVTLKGSRGHKVTLPV 809

BLAST of CSPI01G34240 vs. Swiss-Prot
Match: SBT22_ARATH (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana GN=SBT2.2 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-62
Identity = 135/305 (44.26%), Postives = 193/305 (63.28%), Query Frame = 1

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G+ A+F N+AP +  +S+RGPD  +   + AD+LKP+++APG+ IW AWS 
Sbjct: 555 FGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSS 614

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            +      +G  FA+MSGTSMAAPH+ G+AAL+KQK+  ++PS IASA+STT+  +D  G
Sbjct: 615 AATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKG 674

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
           + I A+    +    + P+TPFD G G V+ + ALDPGL+F T +ED ++FLC + G  P
Sbjct: 675 EAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAP 734

Query: 185 AVVKSATGGQC---NASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 244
            VV + TG  C   NA+IS S   DLNLPSIT+S L   + VQR + N+ G  ETY  S+
Sbjct: 735 -VVFNYTGTNCLRNNATISGS---DLNLPSITVSKLNNTRTVQRLMTNIAGN-ETYTVSL 794

Query: 245 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 304
           I P    +N++P  F++A+ E + L + + A   +   +FG I L G+  HI RIP+S+ 
Sbjct: 795 ITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVT 854

BLAST of CSPI01G34240 vs. TrEMBL
Match: A0A0A0LYA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701260 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 3.2e-171
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD
Sbjct: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP
Sbjct: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV
Sbjct: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300

Query: 301 SAS 304
           SAS
Sbjct: 301 SAS 303

BLAST of CSPI01G34240 vs. TrEMBL
Match: B9RXF5_RICCO (Subtilase, putative OS=Ricinus communis GN=RCOM_0903320 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 4.5e-109
Identity = 198/302 (65.56%), Postives = 247/302 (81.79%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V +F  +  IGEGR+A+F  +AP VSRFSSRGPD+++INR  ADVLKPDILAPGHQIWAA
Sbjct: 517 VSKFCARGAIGEGRVAAFEGRAPIVSRFSSRGPDFLDINRIPADVLKPDILAPGHQIWAA 576

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WSPLSA +P+L G +FAL+SGTSMA PHIVGIAALIKQ +PSWTPSMIASA+STTA  YD
Sbjct: 577 WSPLSALDPILTGDNFALLSGTSMATPHIVGIAALIKQFHPSWTPSMIASALSTTAGNYD 636

Query: 122 MNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 181
             G+LI AEGF++++LYPST FD GAG V+P+ A+DPGLVFP+E+++ I+FLCSLPG+DP
Sbjct: 637 NYGELILAEGFDINSLYPSTHFDLGAGFVNPTRAMDPGLVFPSEFQNYISFLCSLPGIDP 696

Query: 182 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 241
           A+VK+ TG  CN S+  S PA+LNLPS+TIS+L G Q V+R VKNVG K ETY+ SVI P
Sbjct: 697 AIVKATTGEPCNQSL--SSPANLNLPSVTISALRGSQTVERNVKNVGIKPETYLSSVIAP 756

Query: 242 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 301
           NGTTVN++P  F +A +  QN++I+   TH  + F+FG+I+LTGSL+HI RIPLSIL V+
Sbjct: 757 NGTTVNLSPTWFIIAPQGTQNIDIEFHVTHARNEFSFGQIVLTGSLDHIVRIPLSILPVT 816

Query: 302 AS 304
            S
Sbjct: 817 VS 816

BLAST of CSPI01G34240 vs. TrEMBL
Match: A0A067L7P5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02112 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.8e-108
Identity = 200/302 (66.23%), Postives = 237/302 (78.48%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V EF  +A IGEGR+ASF  +AP VSRFSSRGPD+I+INR+ ADVLKPDILAPGHQIWAA
Sbjct: 525 VTEFHARAAIGEGRVASFEGRAPIVSRFSSRGPDFIDINRTPADVLKPDILAPGHQIWAA 584

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WSPLSA EP+L GY FAL+SGTSMA PHI GIAA IKQ  P WTPSMI+SAMSTTATKYD
Sbjct: 585 WSPLSALEPMLTGYKFALLSGTSMATPHIGGIAAFIKQMNPFWTPSMISSAMSTTATKYD 644

Query: 122 MNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 181
             G  I AEGF++++ YPST FD GAGLV+P+ ++DPGLVF +E+ED I+FLCSLP ++ 
Sbjct: 645 NYGQPILAEGFDINSFYPSTHFDLGAGLVNPTRSVDPGLVFTSEFEDYISFLCSLPNINR 704

Query: 182 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 241
             VK ATG  CN     SHPA+LNLPS+TIS+L G Q VQR VKNVG K ETY+ SV+ P
Sbjct: 705 TTVKVATGESCNQLF--SHPANLNLPSVTISALKGSQTVQRSVKNVGSKPETYLGSVMSP 764

Query: 242 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 301
           NGTTV ++P  FT+A +  Q++EIQ + T   D F+FGEI+ TGSLNHI RIPLS+  VS
Sbjct: 765 NGTTVTLSPTWFTIAPQGTQHIEIQFLVTKAGDEFSFGEIVFTGSLNHIVRIPLSVFPVS 824

Query: 302 AS 304
            S
Sbjct: 825 IS 824

BLAST of CSPI01G34240 vs. TrEMBL
Match: A0A061GNV8_THECC (PA-domain containing subtilase family protein OS=Theobroma cacao GN=TCM_030299 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.2e-107
Identity = 197/300 (65.67%), Postives = 241/300 (80.33%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           VREF  +A IG+GR+ASF  QAP VSRFSSRGPD+I+IN+  ADVLKPDILAPGH+IWAA
Sbjct: 523 VREFNARAAIGDGRVASFCGQAPIVSRFSSRGPDFIDINKDPADVLKPDILAPGHEIWAA 582

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WSP+SA +P+L GY+FAL+SGTSMAAPH+ GIAALIKQKYPSWTPSMIASA+STTA+K+D
Sbjct: 583 WSPMSALDPILSGYNFALLSGTSMAAPHVAGIAALIKQKYPSWTPSMIASAISTTASKFD 642

Query: 122 MNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 181
            NG+LI+AEG ++ +LY ST FD GAG VSPS A+DPGLV  +E+ED I+FLCS+P +D 
Sbjct: 643 NNGELIRAEGSDVGSLYSSTYFDSGAGFVSPSRAMDPGLVLSSEFEDYISFLCSIPNIDR 702

Query: 182 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 241
             +++ATG  C+ S+    PA+LNLPS+TIS+L     V+R  KNV  K ETYV   IPP
Sbjct: 703 FAIRAATGVWCSRSL--GLPANLNLPSVTISALERSVTVRRSFKNVATKPETYVSLAIPP 762

Query: 242 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 301
           NGTT+ +NPP F +A EE Q+L+I+I A   T+ F+FGEIILTGSLNHI RIPLSI  VS
Sbjct: 763 NGTTITLNPPWFAIAPEETQDLDIEINAIQSTNQFSFGEIILTGSLNHIVRIPLSIRPVS 820

BLAST of CSPI01G34240 vs. TrEMBL
Match: W9RY25_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 8.0e-106
Identity = 192/309 (62.14%), Postives = 245/309 (79.29%), Query Frame = 1

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           +VR F+ KA IGEGR+ASF +QAPTVSRFSSRGPD+I+  R+  DVLKPDILAPG ++WA
Sbjct: 520 LVRRFRAKAAIGEGRVASFKSQAPTVSRFSSRGPDFIDAKRNPTDVLKPDILAPGQRVWA 579

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSP+SAS+P+L+G  FAL+SGTSMA PHI GIAALIKQ  PSWTPSMI+SA+ TTATKY
Sbjct: 580 AWSPISASDPILEGLSFALVSGTSMATPHIAGIAALIKQYNPSWTPSMISSAIDTTATKY 639

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTE--------YEDNINF 180
           D +G +I AEGF++ +LYPSTPF+ G+GLV+PS A+DPGLVF +E        Y+D + F
Sbjct: 640 DNHGQVIMAEGFSIGSLYPSTPFELGSGLVTPSCAIDPGLVFSSESWGVDEAGYKDYVAF 699

Query: 181 LCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVE 240
           LCSLPG++PA +K A G  CN S+  +HPA+LNLPSITIS+L+G   VQR V+NV  + E
Sbjct: 700 LCSLPGMNPATIKIAIGESCNYSL--THPANLNLPSITISALIGSLSVQRTVRNVRNEPE 759

Query: 241 TYVWSVIPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIAR 300
           TY+ +V+PPNGTTV++NPP FT+A +  Q L++Q+  T   + F+FGEIILTGSL HI +
Sbjct: 760 TYLCAVLPPNGTTVSLNPPWFTIAPQGTQELDVQLNVTKAMNDFSFGEIILTGSLGHIVK 819

Query: 301 IPLSILVVS 302
           IPLS+  +S
Sbjct: 820 IPLSVFPIS 826

BLAST of CSPI01G34240 vs. TAIR10
Match: AT1G62340.1 (AT1G62340.1 PA-domain containing subtilase family protein)

HSP 1 Score: 347.8 bits (891), Expect = 6.7e-96
Identity = 173/297 (58.25%), Postives = 218/297 (73.40%), Query Frame = 1

Query: 4   EFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWS 63
           +F  +A IGEGR + F  +AP VSRFSSRGP +I+  RS  DVLKPDILAPGHQIW AWS
Sbjct: 538 QFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWGAWS 597

Query: 64  PLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMN 123
             SA +P+L G  FA++SGTSMA PHI GI ALIKQ  PSWTP+MIASA+STTA +YD N
Sbjct: 598 LPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEYDSN 657

Query: 124 GDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDPAV 183
           G++I AE + L  L+PS  FD GAG V+P+ ALDPGLV P  +ED I+FLCSLP + PA 
Sbjct: 658 GEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNISPAT 717

Query: 184 VKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPPNG 243
           ++ ATG  C  ++  SHPA+LN PS+TIS+L    VV+R  ++V  K ETY+ SV+PPNG
Sbjct: 718 IRDATGVLCTTTL--SHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLPPNG 777

Query: 244 TTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 301
           TTV + P  FTV  ++ Q+L+I+   T   + FTFGE++LTGSLNHI RIPLS+  +
Sbjct: 778 TTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI 832

BLAST of CSPI01G34240 vs. TAIR10
Match: AT2G19170.1 (AT2G19170.1 subtilisin-like serine protease 3)

HSP 1 Score: 250.8 bits (639), Expect = 1.1e-66
Identity = 131/300 (43.67%), Postives = 180/300 (60.00%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V+ FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG+ IWAA
Sbjct: 514 VKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAA 573

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           W P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 574 WCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 633

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G L+QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED + FLC+ PG
Sbjct: 634 RAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPG 693

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +    +++ T   CN  +   HP++ N PSI +S LVG Q V R+V NV    ETY  + 
Sbjct: 694 ISAHEIRNYTNTACNYDM--KHPSNFNAPSIAVSHLVGTQTVTRKVTNVAEVEETYTITA 753

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 299
                  + +NPP  T+     +   + +     +  ++FGE+ L GS  H  RIP+  L
Sbjct: 754 RMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFGEVKLKGSRGHKVRIPVVAL 811

BLAST of CSPI01G34240 vs. TAIR10
Match: AT5G44530.1 (AT5G44530.1 Subtilase family protein)

HSP 1 Score: 244.2 bits (622), Expect = 1.0e-64
Identity = 137/302 (45.36%), Postives = 189/302 (62.58%), Query Frame = 1

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G  A+F N+AP V  +S+RGPD  + + + ADVLKP+++APG+ IW AWS 
Sbjct: 540 FGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSS 599

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            S      +G  FA+MSGTSMAAPH+ G+AALIKQ YP +TPS I+SA+STTA   D  G
Sbjct: 600 ASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKG 659

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
             I A+    +   +LY +TP D G+G V+ + ALDPGLVF T +ED I+FLC + G D 
Sbjct: 660 SPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFEDYISFLCGINGSD- 719

Query: 185 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 244
            VV + TG +C A+ +     DLNLPSIT+S+L G Q  QR ++N+ G  ETY     PP
Sbjct: 720 TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNIAGN-ETYNVGWSPP 779

Query: 245 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 304
            G ++ ++P  F++A  E Q L + +  T  +   +FG I L G+  HI  IP++++   
Sbjct: 780 YGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNTGHIVNIPVTVIAKI 839

BLAST of CSPI01G34240 vs. TAIR10
Match: AT4G30020.1 (AT4G30020.1 PA-domain containing subtilase family protein)

HSP 1 Score: 242.7 bits (618), Expect = 3.0e-64
Identity = 130/297 (43.77%), Postives = 178/297 (59.93%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V++FK +  IG+G        AP V+ FS+RGP+  + +   AD+LKPDILAPG  IW+A
Sbjct: 515 VKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSA 574

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WS     E    G  FAL+SGTSMAAPHI GIAAL+KQK+P W+P+ I SA+ TT+T  D
Sbjct: 575 WSANGTDEANYIGEGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVID 634

Query: 122 MNGDLIQAEGFN---LHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPG 181
             G  +QA+ ++      L  +TPFD+G+G V+PS ALDPGL+F   YED I FLC+ PG
Sbjct: 635 RAGRPLQAQQYSETETVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTTPG 694

Query: 182 VDPAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 241
           +D   +K+ T   CN  +   HP++ N PSI IS LV  Q V RRV NV  + ETY  + 
Sbjct: 695 IDAHEIKNFTNTPCNFKM--VHPSNFNTPSIAISHLVRTQTVTRRVTNVAEEEETYTITS 754

Query: 242 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPL 296
                  + ++PP  TV A   +   + +     T  ++FG++ L GS  H   +P+
Sbjct: 755 RMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSVTGAYSFGQVTLKGSRGHKVTLPV 809

BLAST of CSPI01G34240 vs. TAIR10
Match: AT4G20430.1 (AT4G20430.1 Subtilase family protein)

HSP 1 Score: 241.5 bits (615), Expect = 6.7e-64
Identity = 135/305 (44.26%), Postives = 193/305 (63.28%), Query Frame = 1

Query: 5   FKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAAWSP 64
           F   A I  G+ A+F N+AP +  +S+RGPD  +   + AD+LKP+++APG+ IW AWS 
Sbjct: 555 FGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSS 614

Query: 65  LSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNG 124
            +      +G  FA+MSGTSMAAPH+ G+AAL+KQK+  ++PS IASA+STT+  +D  G
Sbjct: 615 AATESTEFEGESFAMMSGTSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKG 674

Query: 125 DLIQAEGFNLH---ALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 184
           + I A+    +    + P+TPFD G G V+ + ALDPGL+F T +ED ++FLC + G  P
Sbjct: 675 EAIMAQRAYANPDQTISPATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAP 734

Query: 185 AVVKSATGGQC---NASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSV 244
            VV + TG  C   NA+IS S   DLNLPSIT+S L   + VQR + N+ G  ETY  S+
Sbjct: 735 -VVFNYTGTNCLRNNATISGS---DLNLPSITVSKLNNTRTVQRLMTNIAGN-ETYTVSL 794

Query: 245 IPPNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSIL 304
           I P    +N++P  F++A+ E + L + + A   +   +FG I L G+  HI RIP+S+ 
Sbjct: 795 ITPFDVLINVSPTQFSIASGETKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSVT 854

BLAST of CSPI01G34240 vs. NCBI nr
Match: gi|778665448|ref|XP_004145469.2| (PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 4.5e-171
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 463 MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 522

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 523 AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 582

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD
Sbjct: 583 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 642

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP
Sbjct: 643 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 702

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV
Sbjct: 703 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 762

Query: 301 SAS 304
           SAS
Sbjct: 763 SAS 765

BLAST of CSPI01G34240 vs. NCBI nr
Match: gi|700211754|gb|KGN66850.1| (hypothetical protein Csa_1G701260 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 4.5e-171
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD
Sbjct: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP
Sbjct: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV
Sbjct: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300

Query: 301 SAS 304
           SAS
Sbjct: 301 SAS 303

BLAST of CSPI01G34240 vs. NCBI nr
Match: gi|659118464|ref|XP_008459133.1| (PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo])

HSP 1 Score: 583.2 bits (1502), Expect = 2.7e-163
Identity = 291/302 (96.36%), Postives = 295/302 (97.68%), Query Frame = 1

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           MVREFKGKAGIGEGRIASFG +APTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA
Sbjct: 494 MVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 553

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSPLSASEPLLKGY FALMSGTSMAAPHIVG+AALIKQKYPSWTPSMIASAMSTTATKY
Sbjct: 554 AWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAALIKQKYPSWTPSMIASAMSTTATKY 613

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSP+NALDPGLVFPTEYED INFLCSLPGVD
Sbjct: 614 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNALDPGLVFPTEYEDYINFLCSLPGVD 673

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVV+RRVKNVGGKVETYV SVIP
Sbjct: 674 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVRRRVKNVGGKVETYVCSVIP 733

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGTTVNI PP FTVAAEEVQNLEIQIIATHKTDHFTFGEI LTGSLNHIARIPLSILVV
Sbjct: 734 PNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDHFTFGEIFLTGSLNHIARIPLSILVV 793

Query: 301 SA 303
           SA
Sbjct: 794 SA 795

BLAST of CSPI01G34240 vs. NCBI nr
Match: gi|1009108180|ref|XP_015883427.1| (PREDICTED: subtilisin-like protease SBT2.4 [Ziziphus jujuba])

HSP 1 Score: 416.0 bits (1068), Expect = 5.7e-113
Identity = 201/301 (66.78%), Postives = 247/301 (82.06%), Query Frame = 1

Query: 1   MVREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWA 60
           +V  F  +A IGEGRIA F +Q PTVSRFSSRGPD+I++ R+ ADVLKPDILAPGHQ+WA
Sbjct: 515 LVTRFTARAAIGEGRIACFKDQVPTVSRFSSRGPDFIDVKRNPADVLKPDILAPGHQVWA 574

Query: 61  AWSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKY 120
           AWSP+SA EP+L GY+F L+SGTSMA PH+ GIAAL+KQ  PSWTPSMIASAM+TTA+KY
Sbjct: 575 AWSPISALEPILMGYNFGLLSGTSMATPHVAGIAALLKQYNPSWTPSMIASAMATTASKY 634

Query: 121 DMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVD 180
           D +GDLI+AEG ++ +LYPSTPFDFG+GL+SP +AL PGLVF +EYED + FLCSLP ++
Sbjct: 635 DNDGDLIKAEGSSIGSLYPSTPFDFGSGLLSPKSALHPGLVFSSEYEDYVGFLCSLPDIE 694

Query: 181 PAVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIP 240
           PA++++ATG  CN S+   HPA+LN+PSITISSL+G Q+VQR VKNV  K ETY+ SV+P
Sbjct: 695 PAMIRNATGEWCNYSL--GHPANLNVPSITISSLIGSQLVQRTVKNVESKAETYLCSVLP 754

Query: 241 PNGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVV 300
           PNGT VN+ PP FT+A +E Q+L +Q   T     F+FGEIILTGSLNH+ RIPLSIL V
Sbjct: 755 PNGTVVNLYPPWFTIAPQETQDLYMQFNVTEAIGEFSFGEIILTGSLNHVVRIPLSILPV 813

Query: 301 S 302
           S
Sbjct: 815 S 813

BLAST of CSPI01G34240 vs. NCBI nr
Match: gi|255554773|ref|XP_002518424.1| (PREDICTED: subtilisin-like protease SBT2.4 isoform X2 [Ricinus communis])

HSP 1 Score: 402.5 bits (1033), Expect = 6.5e-109
Identity = 198/302 (65.56%), Postives = 247/302 (81.79%), Query Frame = 1

Query: 2   VREFKGKAGIGEGRIASFGNQAPTVSRFSSRGPDYININRSLADVLKPDILAPGHQIWAA 61
           V +F  +  IGEGR+A+F  +AP VSRFSSRGPD+++INR  ADVLKPDILAPGHQIWAA
Sbjct: 517 VSKFCARGAIGEGRVAAFEGRAPIVSRFSSRGPDFLDINRIPADVLKPDILAPGHQIWAA 576

Query: 62  WSPLSASEPLLKGYHFALMSGTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYD 121
           WSPLSA +P+L G +FAL+SGTSMA PHIVGIAALIKQ +PSWTPSMIASA+STTA  YD
Sbjct: 577 WSPLSALDPILTGDNFALLSGTSMATPHIVGIAALIKQFHPSWTPSMIASALSTTAGNYD 636

Query: 122 MNGDLIQAEGFNLHALYPSTPFDFGAGLVSPSNALDPGLVFPTEYEDNINFLCSLPGVDP 181
             G+LI AEGF++++LYPST FD GAG V+P+ A+DPGLVFP+E+++ I+FLCSLPG+DP
Sbjct: 637 NYGELILAEGFDINSLYPSTHFDLGAGFVNPTRAMDPGLVFPSEFQNYISFLCSLPGIDP 696

Query: 182 AVVKSATGGQCNASISHSHPADLNLPSITISSLVGHQVVQRRVKNVGGKVETYVWSVIPP 241
           A+VK+ TG  CN S+  S PA+LNLPS+TIS+L G Q V+R VKNVG K ETY+ SVI P
Sbjct: 697 AIVKATTGEPCNQSL--SSPANLNLPSVTISALRGSQTVERNVKNVGIKPETYLSSVIAP 756

Query: 242 NGTTVNINPPVFTVAAEEVQNLEIQIIATHKTDHFTFGEIILTGSLNHIARIPLSILVVS 301
           NGTTVN++P  F +A +  QN++I+   TH  + F+FG+I+LTGSL+HI RIPLSIL V+
Sbjct: 757 NGTTVNLSPTWFIIAPQGTQNIDIEFHVTHARNEFSFGQIVLTGSLDHIVRIPLSILPVT 816

Query: 302 AS 304
            S
Sbjct: 817 VS 816

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT24_ARATH1.2e-9458.25Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1[more]
SBT25_ARATH2.0e-6543.67Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1[more]
SBT23_ARATH1.8e-6345.36Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana GN=SBT2.3 PE=2 SV=1[more]
SBT26_ARATH5.4e-6343.77Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana GN=SBT2.6 PE=2 SV=1[more]
SBT22_ARATH1.2e-6244.26Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana GN=SBT2.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYA0_CUCSA3.2e-171100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701260 PE=4 SV=1[more]
B9RXF5_RICCO4.5e-10965.56Subtilase, putative OS=Ricinus communis GN=RCOM_0903320 PE=3 SV=1[more]
A0A067L7P5_JATCU3.8e-10866.23Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02112 PE=4 SV=1[more]
A0A061GNV8_THECC3.2e-10765.67PA-domain containing subtilase family protein OS=Theobroma cacao GN=TCM_030299 P... [more]
W9RY25_9ROSA8.0e-10662.14Subtilisin-like protease OS=Morus notabilis GN=L484_014157 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G62340.16.7e-9658.25 PA-domain containing subtilase family protein[more]
AT2G19170.11.1e-6643.67 subtilisin-like serine protease 3[more]
AT5G44530.11.0e-6445.36 Subtilase family protein[more]
AT4G30020.13.0e-6443.77 PA-domain containing subtilase family protein[more]
AT4G20430.16.7e-6444.26 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|778665448|ref|XP_004145469.2|4.5e-171100.00PREDICTED: subtilisin-like protease SBT2.4 [Cucumis sativus][more]
gi|700211754|gb|KGN66850.1|4.5e-171100.00hypothetical protein Csa_1G701260 [Cucumis sativus][more]
gi|659118464|ref|XP_008459133.1|2.7e-16396.36PREDICTED: LOW QUALITY PROTEIN: cucumisin, partial [Cucumis melo][more]
gi|1009108180|ref|XP_015883427.1|5.7e-11366.78PREDICTED: subtilisin-like protease SBT2.4 [Ziziphus jujuba][more]
gi|255554773|ref|XP_002518424.1|6.5e-10965.56PREDICTED: subtilisin-like protease SBT2.4 isoform X2 [Ricinus communis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042335 cuticle development
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G34240.1CSPI01G34240.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 22..170
score: 3.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 23..120
score: 4.6
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 22..160
score: 7.86
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 11..297
score: 3.3E
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 82..92
scor
NoneNo IPR availablePANTHERPTHR10795:SF390SUBTILISIN-LIKE SERINE PROTEASEcoord: 11..297
score: 3.3E