MELO3C014598.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C014598.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
DescriptiontRNA-specific 2-thiouridylase MnmA
Locationchr05 : 1053376 .. 1058149 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTGGATAATATGTTTTTCGCCTAACGGGTTCGTCTTCCGTCCGGAGATGCGACGGCGGTGGATTGAATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTCTGTTATGTTATATGGTTCTTGTGTTTTCTTGCAAATTATGGTAGCTGATATAGTGAATGCTGGTGCAATTGTGAGCAATCAAATGGGCATTTTCAGCTCGAAGGAAATATAGCCTTTTCGTGTGAACGGACTTTAATGGGTTCTGTTTTCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCTTATAAATCCATCTAAACTTGCAAAGGTTAATTTTTCCAACGAATTTTCAAATGAAATAAAGGATTTGTTGGGAAAATTAAACATTATTCAAGATACCCACAAGTAATGTCTTTCTTCTGTAGTTTGTGCTCAAGAGAGATCTTTGAGCTGAAAATGTTATGTAAATTGGTTTTCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTAAGTGCATTTACAACTACAATTTTATCCTATAACTTTGTTGGATGTGTTAACGATATATTAATACATCCAATCAATTTACTTGTAGCTGTTCCAGCTGACCATCTTTCTCATTCATTCTTTTCTAGTAGCACAAACTGGTATTCGGTTCTCATTTATGTCTACTGAATACTGTCTATTTTATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGTATGTTCATTAGATGGACATTGGTTATATCGTCAAGCTTTCTATTAATGTTTATGATTTCTTTTGTATTAATCTAATATATGGCAAGCCCTGCTTTTTCCAGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTATCTGGGAACGATTGCACAGACATTTCTTTTAATCTAACCTGTCCATTAGGGAGTATCGGTTATTCCTTGCTTAACTTTAATATTTTGTGGTAGGGAAAGGGATCATAGTGAGATTTGGTCTTTCATTAGATTTCATGTGTCTCTTTGAGCTTTGATTTCAAAGTCCTTTTTTAATTATTCAATAGGTGGTATCTTTCTCAGTTGGTCCCCCTTTCTTTAGTTAGGGTGTTTTCTGTGGGTTGTTTTTTTTGTATGCCCTTGTATTCTTGTGATTTCTCATGAAAAAATTGTAGTGGATTGTTTTTGTAACCCTTTAGGTAGGACACTATTTTGCTATTTTCATGACTTTTTTCTATATTCTACACTACACATGGCAGTCAGTAACGCCTATAGTAATTTAACTTGAAGTTTCATGTATAAAGTCAGTGGAATAGATATGACTAATATTGCATTATACGGCCACTATTTGTTGCAATCCTTCAGGAAACAACATCATTAATAAGCTTTATGATTTATATATCGCTTACCTAGTTCAAGGTTTATCATCAAACACATTTATCCCTTCCTTGTTCCTTCTTGCTCTAAAATAAAGATTTCTTATTTTACTTGATTATTATTGTTCTTAGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGTTTGTTCAAGCTAATTTTATAGTATTTATTATGATGTATTTTTTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGATGTTCAAGTTCAACTATTGTAAATTTGTACAATTTGGTACTGGCCTTCTAAGCTTTGTACAGACATTAACACCTAGTGGCTAACATTGGTTGCATTCAGTTGAGAAATTTATGTATTTAATAAAAGCTAAAAACTGTATTACAGACTTTCGCAAGCCAAATGATGTAGATAACATTTTAGAGATTTGTGGATGTTATATTGCAGTTTGAACCCATTTAGTTTTTACTATTCTCGTGTGCAAAACTTATATAAGTGCCTCAATTTATTAGTTCATATCATGCCAACTCCAATCCCAACTAGCTATATTCTTTCAACTTATGCTCTATCTTTTCTGAAGGAAAAAGGAATACTTGTGTGCTTGTTATCTTGTTAATATGTTTTTCTAGGGTTGTGACTTCAGCAATATTTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTACTTTGGATGTGGTGATTCATACATTTTTTTTTTTGTTTGTTTGTCTTTTTTCACGGGAGGCTAATGACTTTCTCAAAATTCATTGGTCAATGCTGATCTATTTCTAATATTTTGGTGTTGCTACTTTATATTTTAGAATATTCTTTACTACTTTTTAGAACAATTTATAGTAACATAGGCTGGCTTAATGGATTTAGTACAAGATTTGCCAACTCTTGTTGAAATTTTCTGATGGCTATACTTTTCCTGAGCAAGTGTTTTATTTCCTTATTCTTCATGACAGCAACAATTTTCATTCATCCTGTTTCTGAAGATGGGTCTCCTGTTTAAGGTATCTTGTGGTAGAATTCATGTAGAACCTGTTTCTTTGATGTGTAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTCTCAGTTTCTTTTCTTTAAGAATAAATTTGATGATCTCTAGCTGTTGTATGGCCATTTTACCAAAACTTCAAGAACATCTTCAACTCTATTTGGTGCAGAGATTTGAGCATTCCGATGTGCCAGTTTAGTTTTTAGTGACCGATTACATTGGGATTATTTCCAATCTGATGCTTCACAAGCTCACATTCTTTACTTACGTCTCTTGCTGAGCTAACAATGACGATTTCAGCTTTTTTGCCTTCGTCTAATGCAGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTATAGTTTCAGCTTCCATCTCCCATATTTCTTGTTAGTTTCACTACTTCAAGTTGGCCTGGCCATAACGATTTACCATAAAAGGAAAATTTCTTAACTTTTTTACAAACCTTTCCAGAGAACCCATCAGTTTATTCCTGCAAGTTTGTGTTGGAAGTTTGACAAGCATGCTTGGCAATATTGATAATCCGGAAATGTTGACTATTACATAGAAAAGTAATTTATCTTCCATGATTATTTAATAGAACAAGCATCTCGATATCATCGATTTTTCTTTCAAACAAAGGATTTAAGTTTAGAATTCAATTTTATCTGTTAAGGGACTTTTTTTGGGGGCCTTTCTCTTTGAGAGGGGAAAATGTATTAAGATTAAGTAGATCTAGAAATGTTATGGGGAGGCTTTGTCTGCTATATCCCGCCCATTTGCTTTGAGATTGTGTAATTGGACTCGAGTTCTCATCTGTCATTTCCAGTTCATTGTTTCATAATTTTATTCTATTTCTAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAGGAGAAGTATCTTTTGAGTGGACTATCACATAATTGCATGTTTGTAATGGAGCATATATCATGGATAAAATAGTTAAGATGTAAGAACTTTTAAAATTAGAGGTCTAAATTGAAATCACGAGGT

mRNA sequence

AAATTGGATAATATGTTTTTCGCCTAACGGGTTCGTCTTCCGTCCGGAGATGCGACGGCGGTGGATTGAATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAGGAGAAGTATCTTTTGAGTGGACTATCACATAATTGCATGTTTGTAATGGAGCATATATCATGGATAAAATAGTTAAGATGTAAGAACTTTTAAAATTAGAGGTCTAAATTGAAATCACGAGGT

Coding sequence (CDS)

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAG

Protein sequence

MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSLPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Homology
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BLAST of MELO3C014598.2 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0 

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of MELO3C014598.2 vs. NCBI nr
Match: XP_004142116.1 (PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_4G291390 [Cucumis sativus])

HSP 1 Score: 1054.3 bits (2725), Expect = 1.4e-304
Identity = 514/539 (95.36%), Postives = 525/539 (97.40%), Query Frame = 0 

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]
BLAST of MELO3C014598.2 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 969.5 bits (2505), Expect = 4.6e-279
Identity = 472/538 (87.73%), Postives = 498/538 (92.57%), Query Frame = 0 

Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVAVVHLSEDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]
BLAST of MELO3C014598.2 vs. NCBI nr
Match: XP_023513286.1 (uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 966.8 bits (2498), Expect = 3.0e-278
Identity = 471/538 (87.55%), Postives = 496/538 (92.19%), Query Frame = 0 

Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRV P VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+S  
Sbjct: 1   MLRLRVKP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESYS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181
           FENFWSECPWDDDLKYA+AVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVAVVHLSEDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]
BLAST of MELO3C014598.2 vs. NCBI nr
Match: XP_022985745.1 (uncharacterized protein LOC111483706 [Cucurbita maxima])

HSP 1 Score: 958.7 bits (2477), Expect = 8.1e-276
Identity = 469/538 (87.17%), Postives = 494/538 (91.82%), Query Frame = 0 

Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRV P VWC++PISHFTSL L PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 1   MLRLRVKP-VWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAV-HKASESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181
           FENFWSECPWDDDLKYAKAVCD++DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR FRVGSLKWLSG+ PD+FTGLQCKVRHGP FYSC LTME ++D  EDVAVV LSEDD
Sbjct: 361 RRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]
BLAST of MELO3C014598.2 vs. TAIR10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases)

HSP 1 Score: 716.1 bits (1847), Expect = 1.7e-206
Identity = 352/477 (73.79%), Postives = 394/477 (82.60%), Query Frame = 0 

Query: 28  SILRPLLRPHNP-------------RLFFTRFSASSAVQHNTSQSSLP-KPLSDAD---- 87
           ++ +P L P  P             R+F + FS+SS+ Q  + QSS P  PL  ++    
Sbjct: 4   AVAKPFLSPSPPLFRLPLSSISISFRVFPSFFSSSSSSQLPSPQSSNPITPLISSNRNYS 63

Query: 88  ---FL--PFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN 147
              F+   +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FEN
Sbjct: 64  RHAFIVDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFEN 123

Query: 148 FWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNT 207
           FW++CPW+DDLKYAK VC+Q+DVPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNT
Sbjct: 124 FWNQCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNT 183

Query: 208 RIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQ 267
           RIKFGAFMDAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ
Sbjct: 184 RIKFGAFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQ 243

Query: 268 NQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGV 327
            QLKRLLFPLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+
Sbjct: 244 TQLKRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGI 303

Query: 328 ILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRR 387
           ILEAESGDFLG HRGFWFYT+GQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR
Sbjct: 304 ILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRR 363

Query: 388 VFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGL 447
           +FRVGSL+W SG P  +   L+CKVRHGPGFYSC+  ME    G  DVAVVHL EDDQGL
Sbjct: 364 IFRVGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGL 423

Query: 448 AAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 482
           AAGQFAAFY+G +CIGSGVILESWDDQ FPVC KA++ A +EDK+KLGKP+KI   P
Sbjct: 424 AAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMP 476

[Close]
BLAST of MELO3C014598.2 vs. Swiss-Prot
Match: sp|Q660I8|MNMA_BORBP (tRNA-specific 2-thiouridylase MnmA OS=Borreliella bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.8e-100
Identity = 168/365 (46.03%), Postives = 250/365 (68.49%), Query Frame = 0 

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++GMP D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of MELO3C014598.2 vs. Swiss-Prot
Match: sp|O51625|MNMA_BORBU (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 9.9e-100
Identity = 168/365 (46.03%), Postives = 250/365 (68.49%), Query Frame = 0 

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of MELO3C014598.2 vs. Swiss-Prot
Match: sp|B7J2N7|MNMA_BORBZ (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 9.9e-100
Identity = 168/365 (46.03%), Postives = 250/365 (68.49%), Query Frame = 0 

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 443 GVILE 447
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of MELO3C014598.2 vs. Swiss-Prot
Match: sp|B2S125|MNMA_BORHD (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 352.4 bits (903), Expect = 8.7e-96
Identity = 166/366 (45.36%), Postives = 244/366 (66.67%), Query Frame = 0 

Query: 83  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
              L  K+RHG     C + M  N     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 443 GVILES 448
             IL++
Sbjct: 361 AQILQT 352

[Close]
BLAST of MELO3C014598.2 vs. Swiss-Prot
Match: sp|A1R0A7|MNMA_BORT9 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia turicatae (strain 91E135) OX=314724 GN=mnmA PE=3 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 3.3e-95
Identity = 165/366 (45.08%), Postives = 245/366 (66.94%), Query Frame = 0 

Query: 83  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +++AVLLSGGVDSSVAL ++   G+ +   +YLKIW +++  ++  ECPW +D+ Y +AV
Sbjct: 1   MQIAVLLSGGVDSSVALYIMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWKEDINYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 202
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CKKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNDHYILKQAKDKIKDQSYFLSHLSRKQISKLHFPLGDLLKTE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAQKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V EKDI+NN++++S +     + ++ F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSINYLKQGKQQFLVHKTNWIN--KPLN 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 442
              L  K+RHG     C + M  N     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NGDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 443 GVILES 448
             IL++
Sbjct: 361 AKILKT 352

[Close]
BLAST of MELO3C014598.2 vs. TrEMBL
Match: tr|A0A1S3BNL8|A0A1S3BNL8_CUCME (tRNA-specific 2-thiouridylase MnmA OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0 

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of MELO3C014598.2 vs. TrEMBL
Match: tr|A0A0A0KX16|A0A0A0KX16_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 9.3e-305
Identity = 514/539 (95.36%), Postives = 525/539 (97.40%), Query Frame = 0 

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
           LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 540
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]
BLAST of MELO3C014598.2 vs. TrEMBL
Match: tr|F6GU47|F6GU47_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_06s0004g02080 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 8.6e-226
Identity = 376/474 (79.32%), Postives = 417/474 (87.97%), Query Frame = 0 

Query: 65  LSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN 124
           L +AD  P+LSCS+P   LK+AVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN
Sbjct: 66  LFNADLQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN 125

Query: 125 FWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNT 184
           FWSECPW+DDLKYAKAVCDQ+DVPLEVVHLT+EYW+NVVSYIIEEYRCGRTPNPDVLCNT
Sbjct: 126 FWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNT 185

Query: 185 RIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQ 244
           RIKFGAFMDAIS+ EFDYVASGHYANV+H   DQMDKPSVLELSKDMVKDQTYFLSHLSQ
Sbjct: 186 RIKFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQ 245

Query: 245 NQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGV 304
            QLKRL+FPLGCIPKDEVR LA K +LPN+DRKDSQGICFLGKIKFSEFVARHIGE EG+
Sbjct: 246 IQLKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGI 305

Query: 305 ILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRR 364
           ILEAE+GD LGKHRGFWFYT+GQRQGLRLPGGPWYVVEKD+KNNVVFVSRNYFS DKRRR
Sbjct: 306 ILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRR 365

Query: 365 VFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGL 424
           +FRVGSLKW+S  PP +   LQCKVRHGPGFY+C+ TME  +DGHEDVAVV LSEDDQGL
Sbjct: 366 LFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGL 425

Query: 425 AAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPDSG 484
           AAGQF AFY+GRSCIGSGVILESWDDQGFPVC KA+E A+MEDKSKLGKP+KIKV+P++ 
Sbjct: 426 AAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETP 485

Query: 485 MNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQI 539
              S   D  K+  +  N +A V +++ + S  EA +  PM WL  +R+K LQ+
Sbjct: 486 PKGSDQIDGIKLDRELANSQAAVTDEISRSSEGEARSRFPMNWLQKIREKWLQM 539

[Close]
BLAST of MELO3C014598.2 vs. TrEMBL
Match: tr|W9RR02|W9RR02_9ROSA (tRNA-specific 2-thiouridylase mnmA OS=Morus notabilis OX=981085 GN=L484_015286 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.9e-220
Identity = 390/542 (71.96%), Postives = 441/542 (81.37%), Query Frame = 0 

Query: 3   LRL-RVNPAVWCSVPI-SHFTSLPLSPSILRPLLRPHNP---RLFFTRFSASSAVQHNTS 62
           LRL RV P    S+ I S F SLPL P    P  +PH P    L F  FS S+A   ++S
Sbjct: 49  LRLTRVMPIPSSSLIIQSRFISLPLKPRTRLP-PKPHLPTSKSLVFRLFSVSAASAVSSS 108

Query: 63  QSSLPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIW 122
             S      D D  P+LSCSMP   L+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIW
Sbjct: 109 HHS---KSFDGDIEPYLSCSMPHKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIW 168

Query: 123 FQEDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPN 182
           FQEDFENFWSECPW++DLKYAKAVC QI+VPLEVVHLT+EYW+NVVSYII+EYRCGRTPN
Sbjct: 169 FQEDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPN 228

Query: 183 PDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTY 242
           PDVLCNTRIKFG+FMDAIS+MEFDYVASGHYANV+H   ++    SVLELS+DMVKDQTY
Sbjct: 229 PDVLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTY 288

Query: 243 FLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARH 302
           FLSHLSQ QLKRL+FPLGCI K+EVRKLA KF+LPNKDRKDSQGICFLGKIKFSEFVARH
Sbjct: 289 FLSHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARH 348

Query: 303 IGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYF 362
           IGE EG+ILEAE+GDFLG HRGFWFYT+GQRQGLRLPGGPWYVVEKD+KNNVVF SRNYF
Sbjct: 349 IGEKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYF 408

Query: 363 SFDKRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHL 422
           SFDKRRR+FRVGSLKWL+G+PPD+   LQCKVRHGPGFY+C+ TME  ++  EDVAVV L
Sbjct: 409 SFDKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQL 468

Query: 423 SEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKI 482
           SEDDQGLA GQFAAFY+G+SC+GSG+ILESWDDQGFPVC+KA+E A+MEDKS LGKP+KI
Sbjct: 469 SEDDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKMEDKSLLGKPVKI 528

Query: 483 KVKPDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQ 540
           KVKP+S   E   K  T++    R+      +Q R+        + PMKWL  LR+K  +
Sbjct: 529 KVKPESPTIEPGQKSSTELDRGQRSSHIAAAKQGRQA------LSFPMKWLQRLREKWFR 580

[Close]
BLAST of MELO3C014598.2 vs. TrEMBL
Match: tr|A0A2H5P4Z9|A0A2H5P4Z9_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_102420 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 1.9e-220
Identity = 386/531 (72.69%), Postives = 436/531 (82.11%), Query Frame = 0 

Query: 5   LRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSLPKP 64
           LRVNP       +    SL LS    R L   HNP+L F R   S +V    +Q   P+ 
Sbjct: 2   LRVNPMSMTLSSVKPLLSLFLSRK-PRTLFHSHNPKLIFLRRPISCSVSTAATQIE-PR- 61

Query: 65  LSDADFLPFLSCSMPTYP-LKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFE 124
                   +LSCSMP    LKVAVLLSGGVDSSVALRLLH+AGHSCTAFYLKIWFQEDFE
Sbjct: 62  --------YLSCSMPHQKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFE 121

Query: 125 NFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCN 184
           NFW+ECPW++D+KYAKAVCDQ+DVPLEVVHLT+EYW+NVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 122 NFWTECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCN 181

Query: 185 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLS 244
           TRIKFGAFMDAIS+MEFDYVASGHYA V+H SADQ  KPSVLELSKD VKDQTYFLSHLS
Sbjct: 182 TRIKFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLS 241

Query: 245 QNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEG 304
           Q QLKRL+FPLGCI K+EVRK A +F+LPNKDRKDSQGICFLGKIKFSEFVARHIGE EG
Sbjct: 242 QAQLKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEG 301

Query: 305 VILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR 364
           VILEAE+GDFLGKH GFWFYT+GQRQGLRLPGGPWYVVEKD+KNNVVFVSRNY+SFDK+R
Sbjct: 302 VILEAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKR 361

Query: 365 RVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQG 424
           R+FRVGSLKWLSG+PP+  + LQCKVRHGPGFY+CNL +EH +DG E++AVV LSEDDQG
Sbjct: 362 RLFRVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQG 421

Query: 425 LAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPDS 484
           LAAGQFAAFY+G  CIGSGVILESWDDQGFPVC+KA+E A+MEDKSKLGKPIKIKVKP++
Sbjct: 422 LAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPET 481

Query: 485 GMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKK 535
              E  +    +      N + ++ E+    S +EA++  PM WL  LR+K
Sbjct: 482 -PEEFNSNKGVQSSRNLVNAQNSIAEKSNTTSQEEAISRFPMNWLQKLREK 520

The following BLAST results are available for this feature:
BLAST of MELO3C014598.2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008449720.10.0e+00100.00PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo][more]
XP_004142116.11.4e-30495.36PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis sativus] >KGN5... [more]
XP_022944568.14.6e-27987.73uncharacterized protein LOC111448983 [Cucurbita moschata][more]
XP_023513286.13.0e-27887.55uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo][more]
XP_022985745.18.1e-27687.17uncharacterized protein LOC111483706 [Cucurbita maxima][more]
BLAST of MELO3C014598.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G51310.11.7e-20673.79transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases[more]
BLAST of MELO3C014598.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q660I8|MNMA_BORBP5.8e-10046.03tRNA-specific 2-thiouridylase MnmA OS=Borreliella bavariensis (strain ATCC BAA-2... [more]
sp|O51625|MNMA_BORBU9.9e-10046.03tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ... [more]
sp|B7J2N7|MNMA_BORBZ9.9e-10046.03tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598... [more]
sp|B2S125|MNMA_BORHD8.7e-9645.36tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314... [more]
sp|A1R0A7|MNMA_BORT93.3e-9545.08tRNA-specific 2-thiouridylase MnmA OS=Borrelia turicatae (strain 91E135) OX=3147... [more]
BLAST of MELO3C014598.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3BNL8|A0A1S3BNL8_CUCME0.0e+00100.00tRNA-specific 2-thiouridylase MnmA OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 ... [more]
tr|A0A0A0KX16|A0A0A0KX16_CUCSA9.3e-30595.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1[more]
tr|F6GU47|F6GU47_VITVI8.6e-22679.32Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_06s0004g02080 PE=3 SV=... [more]
tr|W9RR02|W9RR02_9ROSA1.9e-22071.96tRNA-specific 2-thiouridylase mnmA OS=Morus notabilis OX=981085 GN=L484_015286 P... [more]
tr|A0A2H5P4Z9|A0A2H5P4Z9_CITUN1.9e-22072.69Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_102420 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016783sulfurtransferase activity
GO:0016740transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008033tRNA processing
Vocabulary: INTERPRO
TermDefinition
IPR023382Adenine_a_hdrlase_dom
IPR004506tRNA-specific_2-thiouridylase
IPR014729Rossmann-like_a/b/a_fold
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
cellular_component GO:0005575 cellular_component
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0016740 transferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C014598.2.1MELO3C014598.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 80..295
e-value: 2.8E-80
score: 270.8
IPR004506tRNA-specific 2-thiouridylaseTIGRFAMTIGR00420TIGR00420coord: 84..444
e-value: 1.6E-108
score: 360.6
IPR004506tRNA-specific 2-thiouridylaseHAMAPMF_00144tRNA_thiouridyl_MnmAcoord: 83..445
score: 33.15
IPR004506tRNA-specific 2-thiouridylaseCDDcd01998tRNA_Me_transcoord: 84..444
e-value: 3.5045E-157
score: 454.673
NoneNo IPR availableGENE3DG3DSA:2.40.30.10coord: 363..448
e-value: 1.8E-10
score: 42.6
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 84..444
e-value: 1.8E-125
score: 418.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 479..509
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 35..470
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 84..329
IPR023382Adenine nucleotide alpha hydrolase-like domainsGENE3DG3DSA:2.30.30.280coord: 297..358
e-value: 6.3E-19
score: 69.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
MELO3C014598.2Csa4G291390Cucumber (Chinese Long) v2cumedB279
MELO3C014598.2CSPI04G13870Wild cucumber (PI 183967)cpimedB283
MELO3C014598.2CmaCh20G000790Cucurbita maxima (Rimu)cmamedB598
MELO3C014598.2CmoCh20G000830Cucurbita moschata (Rifu)cmomedB585
MELO3C014598.2Lsi10G010590Bottle gourd (USVL1VR-Ls)lsimedB074
MELO3C014598.2Cp4.1LG16g08850Cucurbita pepo (Zucchini)cpemedB324
MELO3C014598.2CsGy4G013450Cucumber (Gy14) v2cgybmedB247
MELO3C014598.2Cla008543Watermelon (97103) v1medwmB408
MELO3C014598.2ClCG02G023280Watermelon (Charleston Gray)medwcgB370
MELO3C014598.2Cla97C02G049370Watermelon (97103) v2medwmbB368
MELO3C014598.2Bhi10G000238Wax gourdmedwgoB485
MELO3C014598.2Carg06908Silver-seed gourdcarmedB0533
MELO3C014598.2CsaV3_4G024220Cucumber (Chinese Long) v3cucmedB286
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
MELO3C014598.2Silver-seed gourdcarmedB0478
MELO3C014598.2Silver-seed gourdcarmedB0917
MELO3C014598.2Cucumber (Chinese Long) v3cucmedB070
MELO3C014598.2Cucumber (Chinese Long) v3cucmedB563
MELO3C014598.2Watermelon (97103) v2medwmbB371
MELO3C014598.2Watermelon (97103) v2medwmbB404
MELO3C014598.2Wax gourdmedwgoB488
MELO3C014598.2Cucumber (Gy14) v2cgybmedB060
MELO3C014598.2Cucumber (Gy14) v2cgybmedB490
MELO3C014598.2Cucumber (Gy14) v1cgymedB062
MELO3C014598.2Cucurbita maxima (Rimu)cmamedB082
MELO3C014598.2Cucurbita maxima (Rimu)cmamedB144
MELO3C014598.2Cucurbita maxima (Rimu)cmamedB392
MELO3C014598.2Cucurbita maxima (Rimu)cmamedB654
MELO3C014598.2Cucurbita moschata (Rifu)cmomedB076
MELO3C014598.2Cucurbita moschata (Rifu)cmomedB131
MELO3C014598.2Cucurbita moschata (Rifu)cmomedB380
MELO3C014598.2Cucurbita moschata (Rifu)cmomedB642
MELO3C014598.2Cucurbita moschata (Rifu)cmomedB939
MELO3C014598.2Cucurbita pepo (Zucchini)cpemedB165
MELO3C014598.2Cucurbita pepo (Zucchini)cpemedB400
MELO3C014598.2Cucurbita pepo (Zucchini)cpemedB366
MELO3C014598.2Cucurbita pepo (Zucchini)cpemedB761
MELO3C014598.2Cucurbita pepo (Zucchini)cpemedB829
MELO3C014598.2Wild cucumber (PI 183967)cpimedB064
MELO3C014598.2Wild cucumber (PI 183967)cpimedB550
MELO3C014598.2Cucumber (Chinese Long) v2cumedB067
MELO3C014598.2Melon (DHL92) v3.6.1medmedB090
MELO3C014598.2Bottle gourd (USVL1VR-Ls)lsimedB230
MELO3C014598.2Bottle gourd (USVL1VR-Ls)lsimedB439
MELO3C014598.2Watermelon (Charleston Gray)medwcgB373
MELO3C014598.2Watermelon (97103) v1medwmB396
MELO3C014598.2Watermelon (97103) v1medwmB389