CmaCh20G000790 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh20G000790 vs. Swiss-Prot
Match: MNMA_BORBZ (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) GN=mnmA PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.7e-100
Identity = 168/365 (46.03%), Postives = 248/365 (67.95%), Query Frame = 1

Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 140 CDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 200 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W++   P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIND-TPTN 300

Query: 380 FTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGS 439
           F   + K+RHG   YSC L +        ++  + L++ DQG++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKL-----ITNNLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

BLAST of CmaCh20G000790 vs. Swiss-Prot
Match: MNMA_BORAP (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) GN=mnmA PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.7e-100
Identity = 169/365 (46.30%), Postives = 247/365 (67.67%), Query Frame = 1

Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 140 CDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 200 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        DK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N     + +R F V  + W++G  P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWING-TPSD 300

Query: 380 FTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGS 439
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKL-----IAKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

BLAST of CmaCh20G000790 vs. Swiss-Prot
Match: MNMA_BORBU (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) GN=mnmA PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.7e-100
Identity = 168/365 (46.03%), Postives = 248/365 (67.95%), Query Frame = 1

Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 140 CDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 200 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W++   P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIND-TPTN 300

Query: 380 FTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGS 439
           F   + K+RHG   YSC L +        ++  + L++ DQG++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKL-----ITNNLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

BLAST of CmaCh20G000790 vs. Swiss-Prot
Match: MNMA_BORBP (tRNA-specific 2-thiouridylase MnmA OS=Borreliella bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) GN=mnmA PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.2e-99
Identity = 166/365 (45.48%), Postives = 248/365 (67.95%), Query Frame = 1

Query: 80  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 140 CDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 200 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQ 379
           F+T+GQR+G+KL  GPW+V+EKD+  N++++S N   L + +R F V  + W++G+ P  
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGM-PSD 300

Query: 380 FTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGS 439
           F   + K+RHG   YSC L +        ++  + L++ D G++ GQFA FY+   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRL-----ITNNLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 440 GVILE 444
             I +
Sbjct: 361 AKIFK 352

BLAST of CmaCh20G000790 vs. Swiss-Prot
Match: MNMA_BORHD (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) GN=mnmA PE=3 SV=2)

HSP 1 Score: 356.7 bits (914), Expect = 4.5e-97
Identity = 169/366 (46.17%), Postives = 246/366 (67.21%), Query Frame = 1

Query: 80  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 139
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 140 CDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 200 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 259
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 319
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 320 FYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQ 379
           F+TIGQR+G+KL  GPW+V EKDI+NN++++S +   L + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 380 FTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGS 439
              L  K+RHG     C + M ++     D+  V L E D G++ GQF  FYQ   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 440 GVILES 445
             IL++
Sbjct: 361 AQILQT 352

BLAST of CmaCh20G000790 vs. TrEMBL
Match: A0A0A0KX16_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 1.0e-281
Identity = 477/537 (88.83%), Postives = 497/537 (92.55%), Query Frame = 1

Query: 1   MLRLRVKP-VWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASESSSA 60
           MLRLRV P VWC++PISHFTSL L PSILRP+LRRH PRLF+TRFSASSAVH  S SSS+
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCD+VDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQ 420
           RR FRVGSLKWLSG  PDQFT LQCKVRHGPSFYSC LTME  ED  EDVAVV LSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 NATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 537
           +  KE + KDDT+IH  L+N++ TVVEQ  EV+ DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

BLAST of CmaCh20G000790 vs. TrEMBL
Match: F6GU47_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g02080 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 1.0e-225
Identity = 392/532 (73.68%), Postives = 433/532 (81.39%), Query Frame = 1

Query: 19  TSLTLRPSILRPVLRRHRPRLFITRFSAS-----SAVHKASESSSAKG----------LS 78
           TSL+LR   LR  +    P+ F   F  +     +    A+ S++A G          L 
Sbjct: 8   TSLSLRNPNLRFCISLFSPKPFSLCFLPNLRPSITTAAVAATSAAASGTTTITTPPSNLF 67

Query: 79  DGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 138
           + DL+P+LSCS+P   LK+AVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW
Sbjct: 68  NADLQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 127

Query: 139 SECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRI 198
           SECPW+DDLKYAKAVCD+VDVPLEVVHLT+EYW+NVVSYIIEEYRCGRTPNPDVLCNTRI
Sbjct: 128 SECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 187

Query: 199 KFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQ 258
           KFGAFMDAIS+ EFDYVASGHYANV+H   DQMDKPSVLELSKDMVKDQTYFLSHLSQ Q
Sbjct: 188 KFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQ 247

Query: 259 LKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVIL 318
           LKRL+FPLGCIPKDEVR LA K +LPN+DRKDSQGICFLGKIKFSEFVARHIGE EG+IL
Sbjct: 248 LKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIIL 307

Query: 319 EAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSF 378
           EAE+GD LGKHRGFWFYTIGQRQGL+LPGGPWYVVEKD++NNVVFVSRNYFSLDKRRR F
Sbjct: 308 EAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLF 367

Query: 379 RVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAA 438
           RVGSLKW+S   P Q   LQCKVRHGP FY+C  TME  ED  EDVAVV LSEDDQGLAA
Sbjct: 368 RVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAA 427

Query: 439 GQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENATK 498
           GQF AFYQGR CIGSGVILESWDDQGFPVCAKALEIA+MEDKSKLGKP+KIKV+PE   K
Sbjct: 428 GQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPK 487

Query: 499 ECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQV 536
             +  D  K+   L N Q  V ++    +  EA +  PM WL  +R+K LQ+
Sbjct: 488 GSDQIDGIKLDRELANSQAAVTDEISRSSEGEARSRFPMNWLQKIREKWLQM 539

BLAST of CmaCh20G000790 vs. TrEMBL
Match: W9RR02_9ROSA (tRNA-specific 2-thiouridylase mnmA OS=Morus notabilis GN=L484_015286 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 1.9e-219
Identity = 386/540 (71.48%), Postives = 437/540 (80.93%), Query Frame = 1

Query: 2   LRL-RVKPVWCTLPI--SHFTSLTLRPSILRPVLRRHRP--RLFITRFSASSAVHKASES 61
           LRL RV P+  +  I  S F SL L+P    P  + H P  +  + R  + SA    S S
Sbjct: 49  LRLTRVMPIPSSSLIIQSRFISLPLKPRTRLPP-KPHLPTSKSLVFRLFSVSAASAVSSS 108

Query: 62  SSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 121
             +K   DGD+EP+LSCSMP   L+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ
Sbjct: 109 HHSKSF-DGDIEPYLSCSMPHKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 168

Query: 122 EDFENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 181
           EDFENFWSECPW++DLKYAKAVC +++VPLEVVHLT+EYW+NVVSYII+EYRCGRTPNPD
Sbjct: 169 EDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPNPD 228

Query: 182 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 241
           VLCNTRIKFG+FMDAIS+MEFDYVASGHYANV+H   ++    SVLELS+DMVKDQTYFL
Sbjct: 229 VLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTYFL 288

Query: 242 SHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIG 301
           SHLSQ QLKRL+FPLGCI K+EVRKLA KF+LPNKDRKDSQGICFLGKIKFSEFVARHIG
Sbjct: 289 SHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARHIG 348

Query: 302 EDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSL 361
           E EG+ILEAE+GDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKD++NNVVF SRNYFS 
Sbjct: 349 EKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYFSF 408

Query: 362 DKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSE 421
           DKRRR FRVGSLKWL+GL PDQ   LQCKVRHGP FY+C  TME  E+S EDVAVV LSE
Sbjct: 409 DKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQLSE 468

Query: 422 DDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKV 481
           DDQGLA GQFAAFYQG+ C+GSG+ILESWDDQGFPVC KALEIA+MEDKS LGKP+KIKV
Sbjct: 469 DDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKMEDKSLLGKPVKIKV 528

Query: 482 KPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 537
           KPE+ T E   K  T++    ++      +Q  +  S       PMKWL  LR+K  ++F
Sbjct: 529 KPESPTIEPGQKSSTELDRGQRSSHIAAAKQGRQALS------FPMKWLQRLREKWFRIF 580

BLAST of CmaCh20G000790 vs. TrEMBL
Match: A0A067FDJ5_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g042717mg PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 6.7e-217
Identity = 368/467 (78.80%), Postives = 410/467 (87.79%), Query Frame = 1

Query: 67  LEP-FLSCSMPRYP-LKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 126
           +EP +LSCSMP    LKVAVLLSGGVDSSVALRLLH+AGHSCTAFYLKIWFQEDFENFW+
Sbjct: 4   IEPRYLSCSMPHQKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFENFWT 63

Query: 127 ECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIK 186
           ECPW++D+KYAKAVCD+VDVPLEVVHLT+EYW+NVVSYIIEEYRCGRTPNPDVLCNTRIK
Sbjct: 64  ECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 123

Query: 187 FGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQL 246
           FGAFMDAIS+MEFDYVASGHYA V+H SADQ  KPSVLELSKD VKDQTYFLSHLSQ QL
Sbjct: 124 FGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLSQAQL 183

Query: 247 KRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILE 306
           KRL+FPLGCI K+EVRK A +F+LPNKDRKDSQGICFLGKIKFSEFVARHIGE EGVILE
Sbjct: 184 KRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVILE 243

Query: 307 AESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFR 366
           AE+GDFLGKH GFWFYTIGQRQGL+LPGGPWYVVEKD++NNVVFVSRNY+S DK+RR FR
Sbjct: 244 AETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLFR 303

Query: 367 VGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAG 426
           VGSLKWLSGL P++ + LQCKVRHGP FY+C L +E  ED  E++AVV LSEDDQGLAAG
Sbjct: 304 VGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQGLAAG 363

Query: 427 QFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENATKE 486
           QFAAFYQG RCIGSGVILESWDDQGFPVC KALEIA+MEDKSKLGKPIKIKVKPE   +E
Sbjct: 364 QFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPE-TPEE 423

Query: 487 CETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKK 532
             +    +    L N Q ++ E++   + +EA++  PM WL  LR+K
Sbjct: 424 FNSNKGVQSSRNLVNAQNSIAEKSNTTSQEEAISRFPMNWLQKLREK 469

BLAST of CmaCh20G000790 vs. TrEMBL
Match: M5WCR5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004048mg PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 8.2e-215
Identity = 369/492 (75.00%), Postives = 417/492 (84.76%), Query Frame = 1

Query: 45  SASSAVHKASESSSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGH 104
           S+SSAV +     ++    +GDL P+LSCSMP   LKVAVLLSGGVDSSVALRLLHAAGH
Sbjct: 49  SSSSAVPQTPHPPNS---INGDLGPYLSCSMPHQSLKVAVLLSGGVDSSVALRLLHAAGH 108

Query: 105 SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYII 164
           SCTAFYLKIWFQEDFENFWSECPW++DLKYAKAVC++VDVPLEVVHLT EYW+NVVSYII
Sbjct: 109 SCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCNQVDVPLEVVHLTNEYWKNVVSYII 168

Query: 165 EEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLEL 224
           EEYRCGRTPNPDVLCNTRIKFGAFMDAIS M+FDYVASGHYANV+HPSA +MD+ SVLEL
Sbjct: 169 EEYRCGRTPNPDVLCNTRIKFGAFMDAISCMKFDYVASGHYANVVHPSAGEMDEDSVLEL 228

Query: 225 SKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGK 284
           S+DM    TYFLSHLSQ QLK+L+FPLGC+ KDEVRKLA KF+L NK RKDSQGICFLGK
Sbjct: 229 SQDM----TYFLSHLSQAQLKQLIFPLGCLSKDEVRKLATKFDLLNKQRKDSQGICFLGK 288

Query: 285 IKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRN 344
           +KFSEF+ARHIGE EG+ILEAE+GDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+N
Sbjct: 289 LKFSEFIARHIGEKEGIILEAETGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKN 348

Query: 345 NVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDED 404
           NVVFVSRNYFS+DKRRR FRVGSLKWL+G   +Q + LQCKVRHGP F +C L ME    
Sbjct: 349 NVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPQNQISQLQCKVRHGPGFRNCSLIMEPGAY 408

Query: 405 SDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMED 464
             EDVAVV LSEDDQGLAAGQFAAFYQGR C+GSGVILESWDDQGFPVCA+ALEIA+MED
Sbjct: 409 GHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCARALEIARMED 468

Query: 465 KSKLGKPIKIKVKPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKW 524
           KS LGKP+KIKVKPEN   E +  D  ++   LK+   +  +Q  +V+  EA+T  P+ W
Sbjct: 469 KSLLGKPVKIKVKPENPITESDQVDGMELCRGLKHSGISAAKQRMQVSQGEAITRFPVNW 528

Query: 525 LGSLRKKLLQVF 537
           L   R+K L++F
Sbjct: 529 LQKFREKWLRIF 533

BLAST of CmaCh20G000790 vs. TAIR10
Match: AT1G51310.1 (AT1G51310.1 transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases)

HSP 1 Score: 706.1 bits (1821), Expect = 1.7e-203
Identity = 334/421 (79.33%), Postives = 369/421 (87.65%), Query Frame = 1

Query: 67  LEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSEC 126
           ++ +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++C
Sbjct: 69  VDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQC 128

Query: 127 PWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFG 186
           PW+DDLKYAK VC++VDVPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFG
Sbjct: 129 PWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFG 188

Query: 187 AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKR 246
           AFMDAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ QLKR
Sbjct: 189 AFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQLKR 248

Query: 247 LLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAE 306
           LLFPLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+ILEAE
Sbjct: 249 LLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILEAE 308

Query: 307 SGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKRRRSFRVG 366
           SGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKD +NNVVFVSRNY+S+DKRRR FRVG
Sbjct: 309 SGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVG 368

Query: 367 SLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQGLAAGQF 426
           SL+W SG    +   L+CKVRHGP FYSC   ME     D DVAVV L EDDQGLAAGQF
Sbjct: 369 SLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGLAAGQF 428

Query: 427 AAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPENATKECE 486
           AAFY+G  CIGSGVILESWDDQ FPVCAKAL++A +EDK+KLGKP+KI   P  ++ E +
Sbjct: 429 AAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMPTMSSVEAD 485

Query: 487 T 488
           T
Sbjct: 489 T 485

BLAST of CmaCh20G000790 vs. NCBI nr
Match: gi|449448726|ref|XP_004142116.1| (PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis sativus])

HSP 1 Score: 976.9 bits (2524), Expect = 1.5e-281
Identity = 477/537 (88.83%), Postives = 497/537 (92.55%), Query Frame = 1

Query: 1   MLRLRVKP-VWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASESSSA 60
           MLRLRV P VWC++PISHFTSL L PSILRP+LRRH PRLF+TRFSASSAVH  S SSS+
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCD+VDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDDQ 420
           RR FRVGSLKWLSG  PDQFT LQCKVRHGPSFYSC LTME  ED  EDVAVV LSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 NATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 537
           +  KE + KDDT+IH  L+N++ TVVEQ  EV+ DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

BLAST of CmaCh20G000790 vs. NCBI nr
Match: gi|659097615|ref|XP_008449720.1| (PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis melo])

HSP 1 Score: 961.8 bits (2485), Expect = 4.9e-277
Identity = 469/538 (87.17%), Postives = 494/538 (91.82%), Query Frame = 1

Query: 1   MLRLRVKP-VWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAV-HKASESSS 60
           MLRLRV P VWC++PISHFTSL L PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
            K LSD D  PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCD++DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDD 420
           RRR FRVGSLKWLSG+ PD+FTGLQCKVRHGP FYSC LTME ++D  EDVAVV LSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 537
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

BLAST of CmaCh20G000790 vs. NCBI nr
Match: gi|731392853|ref|XP_002282827.2| (PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Vitis vinifera])

HSP 1 Score: 791.2 bits (2042), Expect = 1.1e-225
Identity = 396/550 (72.00%), Postives = 438/550 (79.64%), Query Frame = 1

Query: 1   MLRLRVKPVWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSAS-----SAVHKASE 60
           MLR+   P          TSL+LR   LR  +    P+ F   F  +     +    A+ 
Sbjct: 2   MLRMAATP--------SLTSLSLRNPNLRFCISLFSPKPFSLCFLPNLRPSITTAAVAAT 61

Query: 61  SSSAKG----------LSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHS 120
           S++A G          L + DL+P+LSCS+P   LK+AVLLSGGVDSSVALRLLHAAGHS
Sbjct: 62  SAAASGTTTITTPPSNLFNADLQPYLSCSLPNNRLKIAVLLSGGVDSSVALRLLHAAGHS 121

Query: 121 CTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIE 180
           CTAFYLKIWFQEDFENFWSECPW+DDLKYAKAVCD+VDVPLEVVHLT+EYW+NVVSYIIE
Sbjct: 122 CTAFYLKIWFQEDFENFWSECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIE 181

Query: 181 EYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELS 240
           EYRCGRTPNPDVLCNTRIKFGAFMDAIS+ EFDYVASGHYANV+H   DQMDKPSVLELS
Sbjct: 182 EYRCGRTPNPDVLCNTRIKFGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELS 241

Query: 241 KDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKI 300
           KDMVKDQTYFLSHLSQ QLKRL+FPLGCIPKDEVR LA K +LPN+DRKDSQGICFLGKI
Sbjct: 242 KDMVKDQTYFLSHLSQIQLKRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKI 301

Query: 301 KFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNN 360
           KFSEFVARHIGE EG+ILEAE+GD LGKHRGFWFYTIGQRQGL+LPGGPWYVVEKD++NN
Sbjct: 302 KFSEFVARHIGEKEGIILEAETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNN 361

Query: 361 VVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDS 420
           VVFVSRNYFSLDKRRR FRVGSLKW+S   P Q   LQCKVRHGP FY+C  TME  ED 
Sbjct: 362 VVFVSRNYFSLDKRRRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDG 421

Query: 421 DEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDK 480
            EDVAVV LSEDDQGLAAGQF AFYQGR CIGSGVILESWDDQGFPVCAKALEIA+MEDK
Sbjct: 422 HEDVAVVQLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDK 481

Query: 481 SKLGKPIKIKVKPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWL 536
           SKLGKP+KIKV+PE   K  +  D  K+   L N Q  V ++    +  EA +  PM WL
Sbjct: 482 SKLGKPVKIKVRPETPPKGSDQIDGIKLDRELANSQAAVTDEISRSSEGEARSRFPMNWL 541

BLAST of CmaCh20G000790 vs. NCBI nr
Match: gi|645265499|ref|XP_008238179.1| (PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1 [Prunus mume])

HSP 1 Score: 772.7 bits (1994), Expect = 4.2e-220
Identity = 375/492 (76.22%), Postives = 421/492 (85.57%), Query Frame = 1

Query: 45  SASSAVHKASESSSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGH 104
           S+SSAV +     ++    +GDL P+LSCSMP   LKVAVLLSGGVDSSVALRLLHAAGH
Sbjct: 49  SSSSAVPQTPHPPNS---INGDLGPYLSCSMPHQSLKVAVLLSGGVDSSVALRLLHAAGH 108

Query: 105 SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYII 164
           SCTAFYLKIWFQEDFENFWSECPW++DLKYAKAVC++VDVPLEVVHLT EYW+NVVSYII
Sbjct: 109 SCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCNQVDVPLEVVHLTNEYWKNVVSYII 168

Query: 165 EEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLEL 224
           EEYRCGRTPNPDVLCNTRIKFGAFMDAIS M+FDYVASGHYANV+HPSA +MDK SVLEL
Sbjct: 169 EEYRCGRTPNPDVLCNTRIKFGAFMDAISCMKFDYVASGHYANVVHPSAGEMDKDSVLEL 228

Query: 225 SKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGK 284
           S+DMVKDQTYFLSHLSQ QLK+L+FPLGC+ KDEVRKLA KF+L NK RKDSQGICFLGK
Sbjct: 229 SQDMVKDQTYFLSHLSQAQLKQLIFPLGCLSKDEVRKLATKFDLLNKQRKDSQGICFLGK 288

Query: 285 IKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRN 344
           IKFSEF+ARHIGE EG+ILEAE+GD LG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+N
Sbjct: 289 IKFSEFIARHIGEKEGIILEAETGDLLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKN 348

Query: 345 NVVFVSRNYFSLDKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDED 404
           NVVFVSRNYFS+DKRRR FRVGSLKWL+G  P+Q + LQCKVRHGP F +C L ME    
Sbjct: 349 NVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPPNQISQLQCKVRHGPGFRNCSLIMEPGAY 408

Query: 405 SDEDVAVVLLSEDDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMED 464
             EDVAVV LSEDDQGLAAGQFAAFYQGR C+GSGVILESWDDQGFPVCA+ALEIA+MED
Sbjct: 409 GHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCLGSGVILESWDDQGFPVCARALEIARMED 468

Query: 465 KSKLGKPIKIKVKPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKW 524
           KS LG+P+KIKVKPEN   E +  D  ++ G LK+   +  +Q  +V+  EA+T  P+ W
Sbjct: 469 KSLLGRPVKIKVKPENPITESDQVDGMELCGGLKHSGISAAKQRMQVSQGEAITRFPVNW 528

Query: 525 LGSLRKKLLQVF 537
           L   R+K L +F
Sbjct: 529 LQKFREKWLHIF 537

BLAST of CmaCh20G000790 vs. NCBI nr
Match: gi|703121484|ref|XP_010102337.1| (tRNA-specific 2-thiouridylase mnmA [Morus notabilis])

HSP 1 Score: 770.0 bits (1987), Expect = 2.7e-219
Identity = 386/540 (71.48%), Postives = 437/540 (80.93%), Query Frame = 1

Query: 2   LRL-RVKPVWCTLPI--SHFTSLTLRPSILRPVLRRHRP--RLFITRFSASSAVHKASES 61
           LRL RV P+  +  I  S F SL L+P    P  + H P  +  + R  + SA    S S
Sbjct: 49  LRLTRVMPIPSSSLIIQSRFISLPLKPRTRLPP-KPHLPTSKSLVFRLFSVSAASAVSSS 108

Query: 62  SSAKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 121
             +K   DGD+EP+LSCSMP   L+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ
Sbjct: 109 HHSKSF-DGDIEPYLSCSMPHKRLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQ 168

Query: 122 EDFENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPD 181
           EDFENFWSECPW++DLKYAKAVC +++VPLEVVHLT+EYW+NVVSYII+EYRCGRTPNPD
Sbjct: 169 EDFENFWSECPWEEDLKYAKAVCSQIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPNPD 228

Query: 182 VLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFL 241
           VLCNTRIKFG+FMDAIS+MEFDYVASGHYANV+H   ++    SVLELS+DMVKDQTYFL
Sbjct: 229 VLCNTRIKFGSFMDAISSMEFDYVASGHYANVVHSFTNERGDASVLELSQDMVKDQTYFL 288

Query: 242 SHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIG 301
           SHLSQ QLKRL+FPLGCI K+EVRKLA KF+LPNKDRKDSQGICFLGKIKFSEFVARHIG
Sbjct: 289 SHLSQAQLKRLVFPLGCISKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARHIG 348

Query: 302 EDEGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSL 361
           E EG+ILEAE+GDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKD++NNVVF SRNYFS 
Sbjct: 349 EKEGIILEAETGDFLGMHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYFSF 408

Query: 362 DKRRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSE 421
           DKRRR FRVGSLKWL+GL PDQ   LQCKVRHGP FY+C  TME  E+S EDVAVV LSE
Sbjct: 409 DKRRRIFRVGSLKWLNGLPPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQLSE 468

Query: 422 DDQGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKV 481
           DDQGLA GQFAAFYQG+ C+GSG+ILESWDDQGFPVC KALEIA+MEDKS LGKP+KIKV
Sbjct: 469 DDQGLAVGQFAAFYQGKSCLGSGIILESWDDQGFPVCEKALEIAKMEDKSLLGKPVKIKV 528

Query: 482 KPENATKECETKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 537
           KPE+ T E   K  T++    ++      +Q  +  S       PMKWL  LR+K  ++F
Sbjct: 529 KPESPTIEPGQKSSTELDRGQRSSHIAAAKQGRQALS------FPMKWLQRLREKWFRIF 580