MELO3C008050 (gene) Melon (DHL92) v3.5.1

NameMELO3C008050
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionPutative cysteine desulfurase
Locationchr8 : 7209944 .. 7213434 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAGTTGAAGGTGGAGAATCCCATGTGGAAAAAACCCCAAAGTTTCGCCGGAGAACCTTACACCAGTGACAGAGTGTATGCAGAAGCCGACGTAGAAGATCATGCAGAAAGCGGCACCGCCGAAAGCTATGGAGTTTGCGGCCGGTCAAGATCATTTCAGACGGTCGAGATAGACGGTGTTCCATTTTCCGACAAGGTGGAGGAAAGGCTGGCTTGGTTACGTTCTCAGATTATTGGTGGAGAAGCAGAGATTGATTCTCCATTTGGAGAAAGAAGATTGTGTTATGCTGATCATACAGCTTCCGGTCGTTCTCTTCGTTATATTGAAGATTTCATCCTCAGAAAAGTTCTTCCATTTTACGGTTAATTTCTCCCTCCCTTTCTCTCTACTACCGTTTAAGTTATATTGAAAATTTAGAACTTAGAAGGGTTGTTGGCTACTTTTACTATATAGACCATTTATCGTACGTTACCCATTATAAGAACTGGATGAAAGAACATTCTTTTAAATGTATAGAATAAAGAACGTTGACATGTGCAAATGATACTTCATAACAAACTCAGTATATATATGGCTAATGCAAAATTATTAGAATAGTATCATATGTAATTACGTGTGATGTACAATCAAATATTTTTACTATATAGTTAAAAATAATTTTATGATATATTCTAATAAAAAAAAAAAAAAAACTATGATATGATATGAAACCATTTTTTAAGTGAATTTTAATTTAAAAATTTTTGTTTGCAACTCAACAATAATTGATATAGTATCAGTTTTCTCGCAACCAAAGAAGGGTTGAAACCCTTCTACTTAAAACGAATGAACAATTAAAAAACAAACAAAGAGTTGCTACGTTTTAAAACATTAAATTACAGTATAAAATAAACAATGCTTTTGAATATAGCAAATTTGGAATGTAGCAACAAATATTTTCTGACAATTTTTTTTTTATTTTAAACAATTATGCTAAAAGTAACAACAAAACTTATTTTCTTTAAAAGTAAAAAACAAAACAAAATTATCGCCAACAAAACAATTAACAACGATCAATCTGACCATAATAACCTAATCAATGAATAAAGCATCGATTACCCTTTCAAAAACCTATCAAAAGTCGATAGATTGATCCTAACCCTAAACTTATCTTACTTGCAGGCAACACTCATACATGCGACAGCTACGTAGGACACCATACAACAAAGATGGTGAATGATGCAACTACGTACATCAAGAAATGCTTAGGAGGTGGAGAAGAAGAAGCAATTCTATTCTGTGGACAAGGCACAACTTCCGCCGTTAAAAGACTGCAAGAAGTGATGGGCATTGCAGTGCCATCCATCCTAAGAGAGAGGGTAATAGAAACCCTAAAGGAAGAAGAAAGGTGGGTTGTTTTTGTTGGACCTTATGAGCATCACTCCAACCTCCTCTCGTGGCGGCAGAGCTTAGCTGAGGTAGTAGAAATTGGAATGGATGAGAATGGACTTTTAGACTTTGAAATGCTCAGATCCCAACTTGAAGCATATAAGAAAGTTGGAAATAGACCAATTTTGGGTTCTTTTTCAGCTTGTAGTAATGTCACAGGAATTTATTCAGATACAAAAGCTATTGCTACACTAATCCATCAATATGGAGGCCATGTTTGCTTTGATTTTGCTGCAAGGTACATGAAATTTTGCCTATTTGCTAGAGTTAAAGCATTCACTAAGGATGATAATTTAATCTATTTGTCTATGGTGTAGTGGTCCTTATGTGCAAATTGATATGCAGTCGGGGGAAATTGATGGCTATGATGCGATTTTTCTAAGTACGCACAAGTTTCTTGGAGGGCCTGGATCACCTGGAATCCTTCTGATGAACAAAAGTCTCTACAAGTTAAAATCATCTCCTCCATCAACTTGTGGGGGTGGTACTGTGACTTATGTCAATGGCTTCACTGAAAAGGTAAATGGAAAGATAACCAAATTTTGAGTTTTGTTCGAACCAAATTTTGAGTTTTGTTCGAATGGAATCCAGTAGATTGAAACTGAAATTTTGGTATATTTTGATGTTTGTTCCTTTGCTCACGGAAACTTTCTCCAGGACACACTGTATAATGAAAACATAGAAGAGAGGGAAACTGGTGGAACGCCACAGATTATAGGAATAATAAGAGCAGCTTTGGCTTTTTGGATAAAAGAATATATCGGCTATCAAGAGATAGAGAAGCAAGAGCATCAGTATATAGAAAGGGCTTTGAAGAGACTTCTCCCAAATAAAAGCATCGGTATTTTAGGGAGCACGTCTTCAAAGCGACAGGCGATATTATCTTTTATCATTTACTCTTCGACTAATAATACATCGCCTAACTGTGTCACAGATATGTTATGTAATCCTAACAGCAGAGAAAAGGTTAAAAATATTTACATGTGGGAAGAAACGGGGTGTATGAGAGCCAAACCTCTTCATGGTCCTTTTGTAGCAGCACTCCTGAGCGATTTGTTCGGAGTTCAGGCTCGAGGGGGATGCAGTTGTGCCGGCCCTTATGGTCACAAGCTGCTCAACATTGATGAAGCATGCTCGCAAGCCTACAGAACTGCCATTGCTAAGGTATTAATCAGTCCGATTTTTCAATTTTGTATACCATGAAATAGTGCCATCATCTTTGAATTTTTATCTTTAACAATCTAATAGGGCTATGAGGGAATAAAACCTGGATGGACAAGAGTAAGCTTTCCGTACTACATGCCAAATGAGGAGTTCGAATTCATTTTAAAAGCTTTAGAATTCATAGCTGATTATGGACAAAGATTCCTTCCTTTGTATGCCTTCAATTTGAGAACTGGTAGCTGGACACTGAAGGAAAAGGAGCTCGCCGACCTACTCGGGAAGGAGAATTACAGTGCCAATCACATCTTTGCATTCAAAAACCAGTGTACCAATGCAGAAGCTAGGTTAGCAGCTATAGTATACAAACATAAATCATATTTAGAATCAGCTAAAAAAATTGCCAACCTTCTCCCCAAGTTCCCTCCTGAGAGAGAACTTCACGAGGATATTGAATCCTCTCTATTAAAATTCAGAATTTAGAAAAATACTACATTATAGTTGCATTGTAATCTTGGTTAGCATGATAGTTGAATAACGATAACCAACAAATAAACAAAATGCACTAATGAATTCTTTTTACAATATTTATTTGCCAATTTTCCAGTTATCAGAATCCCCTTCTTCCGATGAAAGGAAAAAAGCATTTCTCATTGAATAATAAGATGTGGTGTGTACAAAACTTTGTGAACGAAACACAAATTCAGAAGACCCGATGAACAAATGATACAATTTAATAATAATACAAACAAATACCCCTCTCGTTGATGCTAACTCAGGCCGAAAGGCAAATCTAGCAACTCACTTATAATCTGCAGAAGCCACTTAACTTCACCTACTTCTCACGGCCTAAAAACAAAATTATTGCTTCATCTTTATTGCCACGAAGTCTCCAGCAAA

mRNA sequence

TGGAGTTGAAGGTGGAGAATCCCATGTGGAAAAAACCCCAAAGTTTCGCCGGAGAACCTTACACCAGTGACAGAGTGTATGCAGAAGCCGACGTAGAAGATCATGCAGAAAGCGGCACCGCCGAAAGCTATGGAGTTTGCGGCCGGTCAAGATCATTTCAGACGGTCGAGATAGACGGTGTTCCATTTTCCGACAAGGTGGAGGAAAGGCTGGCTTGGTTACGTTCTCAGATTATTGGTGGAGAAGCAGAGATTGATTCTCCATTTGGAGAAAGAAGATTGTGTTATGCTGATCATACAGCTTCCGGTCGTTCTCTTCGTTATATTGAAGATTTCATCCTCAGAAAAGTTCTTCCATTTTACGGCAACACTCATACATGCGACAGCTACGTAGGACACCATACAACAAAGATGGTGAATGATGCAACTACGTACATCAAGAAATGCTTAGGAGGTGGAGAAGAAGAAGCAATTCTATTCTGTGGACAAGGCACAACTTCCGCCGTTAAAAGACTGCAAGAAGTGATGGGCATTGCAGTGCCATCCATCCTAAGAGAGAGGGTAATAGAAACCCTAAAGGAAGAAGAAAGGTGGGTTGTTTTTGTTGGACCTTATGAGCATCACTCCAACCTCCTCTCGTGGCGGCAGAGCTTAGCTGAGGTAGTAGAAATTGGAATGGATGAGAATGGACTTTTAGACTTTGAAATGCTCAGATCCCAACTTGAAGCATATAAGAAAGTTGGAAATAGACCAATTTTGGGTTCTTTTTCAGCTTGTAGTAATGTCACAGGAATTTATTCAGATACAAAAGCTATTGCTACACTAATCCATCAATATGGAGGCCATGTTTGCTTTGATTTTGCTGCAAGTGGTCCTTATGTGCAAATTGATATGCAGTCGGGGGAAATTGATGGCTATGATGCGATTTTTCTAAGTACGCACAAGTTTCTTGGAGGGCCTGGATCACCTGGAATCCTTCTGATGAACAAAAGTCTCTACAAGTTAAAATCATCTCCTCCATCAACTTGTGGGGGTGGTACTGTGACTTATGTCAATGGCTTCACTGAAAAGGACACACTGTATAATGAAAACATAGAAGAGAGGGAAACTGGTGGAACGCCACAGATTATAGGAATAATAAGAGCAGCTTTGGCTTTTTGGATAAAAGAATATATCGGCTATCAAGAGATAGAGAAGCAAGAGCATCAGTATATAGAAAGGGCTTTGAAGAGACTTCTCCCAAATAAAAGCATCGGTATTTTAGGGAGCACGTCTTCAAAGCGACAGGCGATATTATCTTTTATCATTTACTCTTCGACTAATAATACATCGCCTAACTGTGTCACAGATATGTTATGTAATCCTAACAGCAGAGAAAAGGTTAAAAATATTTACATGTGGGAAGAAACGGGGTGTATGAGAGCCAAACCTCTTCATGGTCCTTTTGTAGCAGCACTCCTGAGCGATTTGTTCGGAGTTCAGGCTCGAGGGGGATGCAGTTGTGCCGGCCCTTATGGTCACAAGCTGCTCAACATTGATGAAGCATGCTCGCAAGCCTACAGAACTGCCATTGCTAAGGGCTATGAGGGAATAAAACCTGGATGGACAAGAGTAAGCTTTCCGTACTACATGCCAAATGAGGAGTTCGAATTCATTTTAAAAGCTTTAGAATTCATAGCTGATTATGGACAAAGATTCCTTCCTTTGTATGCCTTCAATTTGAGAACTGGTAGCTGGACACTGAAGGAAAAGGAGCTCGCCGACCTACTCGGGAAGGAGAATTACAGTGCCAATCACATCTTTGCATTCAAAAACCAGTGTACCAATGCAGAAGCTAGGTTAGCAGCTATAGTATACAAACATAAATCATATTTAGAATCAGCTAAAAAAATTGCCAACCTTCTCCCCAAGTTCCCTCCTGAGAGAGAACTTCACGAGGATATTGAATCCTCTCTATTAAAATTCAGAATTTAGAAAAATACTACATTATAGTTGCATTGTAATCTTGGTTAGCATGATAGTTGAATAACGATAACCAACAAATAAACAAAATGCACTAATGAATTCTTTTTACAATATTTATTTGCCAATTTTCCAGTTATCAGAATCCCCTTCTTCCGATGAAAGGAAAAAAGCATTTCTCATTGAATAATAAGATGTGGTGTGTACAAAACTTTGTGAACGAAACACAAATTCAGAAGACCCGATGAACAAATGATACAATTTAATAATAATACAAACAAATACCCCTCTCGTTGATGCTAACTCAGGCCGAAAGGCAAATCTAGCAACTCACTTATAATCTGCAGAAGCCACTTAACTTCACCTACTTCTCACGGCCTAAAAACAAAATTATTGCTTCATCTTTATTGCCACGAAGTCTCCAGCAAA

Coding sequence (CDS)

ATGTGGAAAAAACCCCAAAGTTTCGCCGGAGAACCTTACACCAGTGACAGAGTGTATGCAGAAGCCGACGTAGAAGATCATGCAGAAAGCGGCACCGCCGAAAGCTATGGAGTTTGCGGCCGGTCAAGATCATTTCAGACGGTCGAGATAGACGGTGTTCCATTTTCCGACAAGGTGGAGGAAAGGCTGGCTTGGTTACGTTCTCAGATTATTGGTGGAGAAGCAGAGATTGATTCTCCATTTGGAGAAAGAAGATTGTGTTATGCTGATCATACAGCTTCCGGTCGTTCTCTTCGTTATATTGAAGATTTCATCCTCAGAAAAGTTCTTCCATTTTACGGCAACACTCATACATGCGACAGCTACGTAGGACACCATACAACAAAGATGGTGAATGATGCAACTACGTACATCAAGAAATGCTTAGGAGGTGGAGAAGAAGAAGCAATTCTATTCTGTGGACAAGGCACAACTTCCGCCGTTAAAAGACTGCAAGAAGTGATGGGCATTGCAGTGCCATCCATCCTAAGAGAGAGGGTAATAGAAACCCTAAAGGAAGAAGAAAGGTGGGTTGTTTTTGTTGGACCTTATGAGCATCACTCCAACCTCCTCTCGTGGCGGCAGAGCTTAGCTGAGGTAGTAGAAATTGGAATGGATGAGAATGGACTTTTAGACTTTGAAATGCTCAGATCCCAACTTGAAGCATATAAGAAAGTTGGAAATAGACCAATTTTGGGTTCTTTTTCAGCTTGTAGTAATGTCACAGGAATTTATTCAGATACAAAAGCTATTGCTACACTAATCCATCAATATGGAGGCCATGTTTGCTTTGATTTTGCTGCAAGTGGTCCTTATGTGCAAATTGATATGCAGTCGGGGGAAATTGATGGCTATGATGCGATTTTTCTAAGTACGCACAAGTTTCTTGGAGGGCCTGGATCACCTGGAATCCTTCTGATGAACAAAAGTCTCTACAAGTTAAAATCATCTCCTCCATCAACTTGTGGGGGTGGTACTGTGACTTATGTCAATGGCTTCACTGAAAAGGACACACTGTATAATGAAAACATAGAAGAGAGGGAAACTGGTGGAACGCCACAGATTATAGGAATAATAAGAGCAGCTTTGGCTTTTTGGATAAAAGAATATATCGGCTATCAAGAGATAGAGAAGCAAGAGCATCAGTATATAGAAAGGGCTTTGAAGAGACTTCTCCCAAATAAAAGCATCGGTATTTTAGGGAGCACGTCTTCAAAGCGACAGGCGATATTATCTTTTATCATTTACTCTTCGACTAATAATACATCGCCTAACTGTGTCACAGATATGTTATGTAATCCTAACAGCAGAGAAAAGGTTAAAAATATTTACATGTGGGAAGAAACGGGGTGTATGAGAGCCAAACCTCTTCATGGTCCTTTTGTAGCAGCACTCCTGAGCGATTTGTTCGGAGTTCAGGCTCGAGGGGGATGCAGTTGTGCCGGCCCTTATGGTCACAAGCTGCTCAACATTGATGAAGCATGCTCGCAAGCCTACAGAACTGCCATTGCTAAGGGCTATGAGGGAATAAAACCTGGATGGACAAGAGTAAGCTTTCCGTACTACATGCCAAATGAGGAGTTCGAATTCATTTTAAAAGCTTTAGAATTCATAGCTGATTATGGACAAAGATTCCTTCCTTTGTATGCCTTCAATTTGAGAACTGGTAGCTGGACACTGAAGGAAAAGGAGCTCGCCGACCTACTCGGGAAGGAGAATTACAGTGCCAATCACATCTTTGCATTCAAAAACCAGTGTACCAATGCAGAAGCTAGGTTAGCAGCTATAGTATACAAACATAAATCATATTTAGAATCAGCTAAAAAAATTGCCAACCTTCTCCCCAAGTTCCCTCCTGAGAGAGAACTTCACGAGGATATTGAATCCTCTCTATTAAAATTCAGAATTTAG

Protein sequence

MWKKPQSFAGEPYTSDRVYAEADVEDHAESGTAESYGVCGRSRSFQTVEIDGVPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKRQAILSFIIYSSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCTNAEARLAAIVYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI*
BLAST of MELO3C008050 vs. Swiss-Prot
Match: CSD_BACHD (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=csd PE=3 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 6.0e-19
Identity = 95/360 (26.39%), Postives = 155/360 (43.06%), Query Frame = 1

Query: 77  IDSPFGERRLCYADHTASGRS----LRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVN 136
           +D       L Y D  A+ +     +  ++D+  R    +  N H     +G   T    
Sbjct: 13  LDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRR----YNSNVHRGVHTLGTLATDGYE 72

Query: 137 DATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEERWVV 196
            A   I++ +     E I+F  +GTT+A+  +    G A            L E +   +
Sbjct: 73  GAREKIRRFIHASSTEEIIFT-RGTTTAINLVAASYGRA-----------NLGEGDE--I 132

Query: 197 FVGPYEHHSNLLSWRQSL----AEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSF 256
            + P EHHSN++ W+Q      A +  + + ++G +  E      +  K +  +  + + 
Sbjct: 133 VITPMEHHSNIIPWQQVAKATGATLTYLPLQKDGTIKIE------DVEKTISEKTKIVAI 192

Query: 257 SACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKF 316
              SNV G  +  K IA + H++G  +  D A S P+++ID+Q     G D    S HK 
Sbjct: 193 MHVSNVLGTINPVKEIAEIAHRHGAIMLVDGAQSAPHMKIDVQEL---GCDFFAFSGHK- 252

Query: 317 LGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQI 376
           + GP   G+L   K+   L+   P   GG  + +V  +   D+ + E +  +  GGTP I
Sbjct: 253 MAGPTGIGVLYGKKA--HLEKMEPVEFGGEMIDFVGLY---DSTWKE-LPWKFEGGTPII 312

Query: 377 IGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKRQAILSFII 429
            G I    A    E IG  EIEK EH+  + AL RL   + + + G    KR  +++F I
Sbjct: 313 AGAIGLGAAIDFLEDIGLDEIEKHEHELAQYALDRLSELEGMTVYG--PQKRAGLVTFNI 336

BLAST of MELO3C008050 vs. Swiss-Prot
Match: CSD_STRCO (Probable cysteine desulfurase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) GN=csd PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.6e-14
Identity = 77/349 (22.06%), Postives = 145/349 (41.55%), Query Frame = 1

Query: 85  RLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGG 144
           +L Y D+ A+ +  R + D +      +  N H     +    T +   A   + + +  
Sbjct: 29  KLVYLDNAATSQKPRQVLDALSEYYERYNANVHRGVHVLAEEATALYEGARDKVAEFINA 88

Query: 145 GEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLL 204
              + ++F  +  + ++  +  ++G A          +  + +    + +   EHHSN++
Sbjct: 89  PSRDEVIFT-KNASESLNLVANMLGWAD---------DPYRVDHETEIVITEMEHHSNIV 148

Query: 205 SWR----QSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSD 264
            W+    ++ A++   G+ ++G LD   +   +    KV       SF   SN+ G  + 
Sbjct: 149 PWQLLAQRTGAKLRWFGLTDDGRLDLSNIDEVITEKTKVV------SFVLVSNILGTQNP 208

Query: 265 TKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLM 324
            +AI     + G  VC D + + P++ +D+Q+ + D    +  + HK  G P   G+L  
Sbjct: 209 VEAIVRRAQEVGALVCIDASQAAPHMPLDVQALQAD---FVAFTGHKMCG-PTGIGVLWG 268

Query: 325 NKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWI 384
            + L  L+  PP   GG  +  V+  +          E     GTP +   +    A   
Sbjct: 269 RQEL--LEDLPPFLGGGEMIETVSMHSSTYAPAPHKFE----AGTPPVAQAVGLGAAIDY 328

Query: 385 KEYIGYQEIEKQEHQYIERALKRLLPNKSIGILG-STSSKRQAILSFII 429
              IG  +I   EH   E A+KRLL    + I+G +T+ +R A +SF +
Sbjct: 329 LNSIGMDKILAHEHALTEYAVKRLLEVPDLRIIGPTTAEERGAAISFTL 351

BLAST of MELO3C008050 vs. Swiss-Prot
Match: CSD_CHLPN (Probable cysteine desulfurase OS=Chlamydia pneumoniae GN=csd PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.7e-13
Identity = 89/365 (24.38%), Postives = 149/365 (40.82%), Query Frame = 1

Query: 70  IIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCDSYVGHHTTK 129
           I   +A+ + PF      Y D  A+ +  + + D +       Y   +        + T+
Sbjct: 10  IFAAKAKENEPF-----IYLDSAATTQKPQQVIDAVANFYTSSYATVNRAIYSSSRNVTE 69

Query: 130 MVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQ-EVMGIAVPSILRERVIETLKEEE 189
                   ++K +    +  I+F  +GTT+ +  L   V  + +P              +
Sbjct: 70  AYAAVREKVRKWVSAASDSEIVFT-RGTTAGLNLLAISVNDLWIP--------------K 129

Query: 190 RWVVFVGPYEHHSNLLSW----RQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPI 249
             VV V   EHH+N+LSW    R+  + V +I + ++GL+D + L   L    +  + P 
Sbjct: 130 GGVVLVSEAEHHANVLSWEIACRRRGSLVKKIRVHDSGLIDLDDLEKLLNEGAQFVSIPH 189

Query: 250 LGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLS 309
           +      SNVTG     + +A L+H+Y  ++  D A   P++ ID+Q  ++D Y     S
Sbjct: 190 V------SNVTGCVQPLQQVAELVHRYDAYLAVDGAQGAPHLPIDVQLWDVDFY---VFS 249

Query: 310 THKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGG 369
           +HK + GP   G+L   K L  L   PP   GGG +  +      + L       +   G
Sbjct: 250 SHK-IYGPTGIGVLYGKKDL--LDQLPPVE-GGGDMVAIYDHQNPEYL---PAPMKFEAG 309

Query: 370 TPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILG-STSSKRQAI 429
           TP I G++    A    + +  + I  +E        K LL    + ILG S    R A+
Sbjct: 310 TPNIAGVLGLGAALDYLDGLSAKFIYDKEIALTTYLHKELLEIPGVEILGPSIEEPRGAL 338

BLAST of MELO3C008050 vs. Swiss-Prot
Match: CSD_PYRAB (Probable cysteine desulfurase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=csd PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 8.3e-13
Identity = 64/214 (29.91%), Postives = 97/214 (45.33%), Query Frame = 1

Query: 196 PYEHHSNLLSWRQSLAEVVEIGM-----DENGLLDFEMLRSQLEAYKKVGNRPILGSFSA 255
           PYEHHSNLL W Q LA+   + +     D+ G LD      +++  K V  + +      
Sbjct: 111 PYEHHSNLLPW-QRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAKLVAVQHV------ 170

Query: 256 CSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLG 315
            SN  G+  + + +  ++ + G     D A S  ++++D++  + D    +  S HK   
Sbjct: 171 -SNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKAD---FLAFSGHKGPM 230

Query: 316 GPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQIIG 375
           GP   G+L +NK  + +   P    GGGT+  V     K T   E  E     GTP I G
Sbjct: 231 GPTGIGVLYINKEFFDVFEPP--LIGGGTIEDVELCCYKLTEPPERFE----AGTPNIGG 290

Query: 376 IIRAALAFWIKEYIGYQEIEKQEHQYIERALKRL 405
            I  A      E IG  +IEKQE + ++R  + L
Sbjct: 291 AIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGL 307

BLAST of MELO3C008050 vs. Swiss-Prot
Match: CSD_CHLMU (Probable cysteine desulfurase OS=Chlamydia muridarum (strain MoPn / Nigg) GN=csd PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 6.6e-10
Identity = 71/228 (31.14%), Postives = 106/228 (46.49%), Query Frame = 1

Query: 192 VFVGPYEHHSNLLSW----RQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGS 251
           V V   EHH+N+LSW    R+S A V ++ +++ G++D     S LE   K G +  L S
Sbjct: 109 VVVSEAEHHANILSWEIACRRSGATVKKVRVNDEGIIDL----SHLEKLLKQGVQ--LVS 168

Query: 252 FSACSNVTGIYSDTKAIATLIHQYGGHVCFDFA---ASGPYVQIDMQSGEIDGYDAIFLS 311
            +  SNV+G     + +A L+H+YG  +  D A    SGP   +++   ++D Y     S
Sbjct: 169 LAHVSNVSGAVLPVQEVAFLVHRYGALLAIDGAQGVGSGP---LNLSGWDVDFY---AFS 228

Query: 312 THKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGG 371
            HK L  P   G+L   + L  L+S PP   GGG +  V  +  + + Y E    R   G
Sbjct: 229 GHK-LYAPTGIGVLYGKREL--LESLPPVE-GGGDMVVV--YDSESSRYQEP-PLRFEAG 288

Query: 372 TPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGI 413
           TP I G++    A    +Y+        +H     AL R L N+ + I
Sbjct: 289 TPHIAGVLGLGAAI---DYLQALPFSVSDHL---TALTRFLYNRLLTI 311

BLAST of MELO3C008050 vs. TrEMBL
Match: A0A0A0KF70_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483410 PE=4 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 1.2e-289
Identity = 492/520 (94.62%), Postives = 506/520 (97.31%), Query Frame = 1

Query: 130 MVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEER 189
           MV+DATTYIK+CLGGGEEEAILFCGQGTTSA+KRLQEVMGIAVPSILRERVIETLKEEER
Sbjct: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60

Query: 190 WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFS 249
           WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEA KK GNRPILGSFS
Sbjct: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120

Query: 250 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFL 309
           ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDM+SGEIDGYDAIFLSTHKFL
Sbjct: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180

Query: 310 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQII 369
           GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLY ENIEERE GGTPQII
Sbjct: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240

Query: 370 GIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKRQAILSFIIY 429
           GIIRAALAFW+KEYIGYQEIEKQEH+YIERALKRLLPN+SI ILGSTS+KRQAILSFIIY
Sbjct: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300

Query: 430 SSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARG 489
           SSTNN+ PNC+TDML NPNSREKVK +YMWEETG MRAKPLHGPFVAALLSDLFGVQARG
Sbjct: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360

Query: 490 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALE 549
           GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYM NEEFEFILKALE
Sbjct: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420

Query: 550 FIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCTNAEARLAAI 609
           FIADYGQRFLPLYAFNLR GSWTLKEKEL DLLGKENYSA+HIFAFKNQCTNAEA+LAAI
Sbjct: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480

Query: 610 VYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 650
           VYKHKSYLESAK+IANLLPKFPPER LHEDIESSLL FRI
Sbjct: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 520

BLAST of MELO3C008050 vs. TrEMBL
Match: W9R1J9_9ROSA (Putative cysteine desulfurase OS=Morus notabilis GN=L484_017507 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 7.1e-245
Identity = 426/625 (68.16%), Postives = 512/625 (81.92%), Query Frame = 1

Query: 35  SYGVCGRSRSFQTVEIDGVPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADHTAS 94
           S G+C RS SF+++E+D VP S + + RL+WLRSQIIGG  E DSPFG+RRL YADHTAS
Sbjct: 68  SNGLCNRSDSFRSLEMD-VPKSCEDQVRLSWLRSQIIGGNVEYDSPFGKRRLTYADHTAS 127

Query: 95  GRSLRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCG 154
           GRSL YIE+FI+  VLPFYGNTHTCDS+VGH TTKMV++A  YIKKCLGGG+++A+ FCG
Sbjct: 128 GRSLLYIENFIIDNVLPFYGNTHTCDSHVGHRTTKMVHEAADYIKKCLGGGQDDALFFCG 187

Query: 155 QGTTSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVV 214
            GTT+A+KRLQEVMGIAVPSILRER ++ L  EE+WVVFVGP+EHHSNLLSWRQSLAEVV
Sbjct: 188 SGTTAAIKRLQEVMGIAVPSILRERAMKILSNEEKWVVFVGPFEHHSNLLSWRQSLAEVV 247

Query: 215 EIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGH 274
           EIG+DENGLLD E LR +LE+YK   +RPILGSFSA SNVTGI SDTKAIA L+HQYGG 
Sbjct: 248 EIGLDENGLLDMEALRLKLESYK-YAHRPILGSFSASSNVTGINSDTKAIAQLLHQYGGF 307

Query: 275 VCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPST 334
            CFDFAA GPY++IDM+SG++DGYDA+FLS HKFLGGPG+PGI+LM+K+LY+L+SS PST
Sbjct: 308 ACFDFAACGPYLEIDMRSGKMDGYDAVFLSPHKFLGGPGTPGIVLMSKALYRLRSSAPST 367

Query: 335 CGGGTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEH 394
           CGGGTV YVNGF+EKDTLY E+IEERE  GTPQII  IRAALAFW+KEY+GY+ IE+QE+
Sbjct: 368 CGGGTVAYVNGFSEKDTLYYEDIEERENAGTPQIIQTIRAALAFWVKEYVGYEVIERQEN 427

Query: 395 QYIERALKRLLPNKSIGILGSTSSKRQAILSFIIYSSTNNTS---PNCVTDMLCNPNSRE 454
            YI++A+KRLLPNKSI +LG+TS+KRQAILSF+IYS++N+ S    NC  D     N  E
Sbjct: 428 IYIQKAIKRLLPNKSIQVLGNTSAKRQAILSFLIYSTSNSYSRDLKNCCDDNYRTNNENE 487

Query: 455 KVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACSQA 514
           + + +YMW ETG  R KPLHGPFVAALL+DLFG+QARGGC+CAGPYGH LL +DE  S A
Sbjct: 488 E-EGLYMWRETGNSRDKPLHGPFVAALLNDLFGIQARGGCACAGPYGHALLKVDEPHSHA 547

Query: 515 YRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTGSW 574
           YR+AI KG+ G+KPGWTR+SFPYYM N+EFEFIL ALEFIA YGQRFL LY F+LRTG W
Sbjct: 548 YRSAIKKGFLGVKPGWTRISFPYYMANDEFEFILAALEFIATYGQRFLTLYDFSLRTGGW 607

Query: 575 TLKEKELADLLGKENYSANHIFAFK------NQCTNAEARLAAI-VYKHKSYLESAKKIA 634
           T ++K L  L+ K+N S N++ A K       Q  NAE +   I V K   +LE+AK IA
Sbjct: 608 TARKKALLCLV-KKNNSNNNVKAMKTENEVFKQSNNAETKHNGIMVNKFAMFLETAKDIA 667

Query: 635 NLLPKFPPERELHEDIESSLLKFRI 650
           +LLPKFPP+R L E+I+ + L FR+
Sbjct: 668 HLLPKFPPQRRLQEEIDLNFLHFRV 688

BLAST of MELO3C008050 vs. TrEMBL
Match: A0A061DJH6_THECC (Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM_001454 PE=4 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 1.9e-242
Identity = 421/630 (66.83%), Postives = 508/630 (80.63%), Query Frame = 1

Query: 38  VCGRSRSFQTVEIDGVPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRS 97
           +C  S SF+T+E+ GVP  D  +++L+WLRSQIIGG+A+ D+PFG R+L YADHTASGR 
Sbjct: 44  LCHGSESFRTLEM-GVPKVDSTDKKLSWLRSQIIGGDAQFDTPFGTRKLTYADHTASGRC 103

Query: 98  LRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGT 157
           L YIE+FI+  VLPFYGNTHTCDSYVGH TTKMV++A+ Y+K+CLGGG+++AILFCG G+
Sbjct: 104 LHYIENFIVTNVLPFYGNTHTCDSYVGHRTTKMVHEASNYVKRCLGGGQDDAILFCGSGS 163

Query: 158 TSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIG 217
           T+A+KRLQEVMGIAVPSILR+R++  L  E+RWVVFVGPYEHHSNLLSWRQSLAEVVEIG
Sbjct: 164 TAAIKRLQEVMGIAVPSILRDRLLNCLINEKRWVVFVGPYEHHSNLLSWRQSLAEVVEIG 223

Query: 218 MDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCF 277
           +DENGL++ E LR QLE YK   NRP+LGSFSACSNVTGI SDT+ IA L+H YGG VCF
Sbjct: 224 LDENGLINIEALRRQLELYKHA-NRPLLGSFSACSNVTGIQSDTREIAKLVHGYGGFVCF 283

Query: 278 DFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGG 337
           DFAASGPYV+IDM+SGE DGYDAIFLS HKFLGGPGSPGILLMNK+LY+L +SPPSTCGG
Sbjct: 284 DFAASGPYVEIDMRSGEFDGYDAIFLSPHKFLGGPGSPGILLMNKALYQLGASPPSTCGG 343

Query: 338 GTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYI 397
           GTV YVNGF EKDTLY +++EERE  GTPQII IIRAAL FW+KEYIGY+ IEKQE  YI
Sbjct: 344 GTVNYVNGFNEKDTLYVDDVEERENAGTPQIIQIIRAALTFWVKEYIGYEVIEKQEKSYI 403

Query: 398 ERALKRLLPNKSIGILGSTS--SKRQAILSFIIYSSTN------NTSPNCVTDMLCNPNS 457
           ++AL+RLLPN++IGILG+TS  ++RQAILSF+++S+TN      N   N V ++    + 
Sbjct: 404 QQALERLLPNQNIGILGNTSTETERQAILSFLVFSTTNSCPGPVNKEGNHVDEL----DG 463

Query: 458 REKVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACS 517
           R K + ++MW ETG  R KPLHGPFV  LL+DLFG+QARGGC+CAGPYGH LL+IDE  S
Sbjct: 464 RMK-RGLFMWGETGNRRDKPLHGPFVVTLLNDLFGIQARGGCACAGPYGHSLLHIDEPRS 523

Query: 518 QAYRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTG 577
            A+R AI KGY G KPGW RVSFPYYM NEEFEFIL A+EFIA YGQRFL LY FNLRTG
Sbjct: 524 LAFREAIEKGYAGAKPGWARVSFPYYMSNEEFEFILAAIEFIAIYGQRFLSLYHFNLRTG 583

Query: 578 SWTLKEKELADLLGKENYSANHIF----AF------KNQCTNAEARLAAIVYKHKSYLES 637
           SWT K+K L DL+GKE    +H+     AF      ++Q +  +    +++  + SYL+S
Sbjct: 584 SWTFKKKALKDLVGKEMSQGSHVLPLASAFPVTSVDRDQPSQYDDADVSVIRTYASYLKS 643

Query: 638 AKKIANLLPKFPPERELHEDIESSLLKFRI 650
           AK+IA LLPKFP +R LHEDI+  LL +R+
Sbjct: 644 AKRIAALLPKFPSQRRLHEDIDLDLLPYRL 666

BLAST of MELO3C008050 vs. TrEMBL
Match: M5X0L3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003222mg PE=4 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 8.4e-238
Identity = 410/597 (68.68%), Postives = 480/597 (80.40%), Query Frame = 1

Query: 53  VPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPF 112
           +P ++  E RL+WLRSQ+IG  AE DSPFG+RRL YADHTASGRSL YIE+FIL  VLPF
Sbjct: 1   MPRTESEEIRLSWLRSQVIGAAAEFDSPFGKRRLTYADHTASGRSLLYIENFILNNVLPF 60

Query: 113 YGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAV 172
           YGNTHTCDSYVG+ T+KMV++A  YIKKCLGGG EEA+ FCGQGTTSA+KRLQEVMGIAV
Sbjct: 61  YGNTHTCDSYVGNRTSKMVHEAAKYIKKCLGGGPEEALFFCGQGTTSAIKRLQEVMGIAV 120

Query: 173 PSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQ 232
           PSILR+RVI  L  EERW+VFVGPYEHHSNLLSWRQSLAEVVEIG++++GLLD E LR Q
Sbjct: 121 PSILRDRVINNLNTEERWLVFVGPYEHHSNLLSWRQSLAEVVEIGVNDDGLLDLEALRLQ 180

Query: 233 LEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQS 292
           LE YK V NRPILGSFSACSNVTGIYSDT+AIA LIH+YGG  CFDFAASGPYV+I+M+ 
Sbjct: 181 LEKYKYV-NRPILGSFSACSNVTGIYSDTRAIARLIHRYGGFACFDFAASGPYVEIEMRC 240

Query: 293 GEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTL 352
           GE+DGYD +FLS HKFLGGPGSPGIL+M+K+LY+L+S PPSTCGGGTV YVNGF+++DTL
Sbjct: 241 GEMDGYDGVFLSPHKFLGGPGSPGILIMSKALYQLRSLPPSTCGGGTVAYVNGFSDEDTL 300

Query: 353 YNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGI 412
           Y E++EERE GGTPQII  IRAALAFW+KEYIGYQ IEK E  Y++RAL+RLLPN ++ +
Sbjct: 301 YWEDVEERENGGTPQIIQTIRAALAFWVKEYIGYQVIEKHEDFYVKRALERLLPNNNVWV 360

Query: 413 LGSTSSKRQAILSFIIYSSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHG 472
           LG+TS+KRQAI SF+IYS+TN  S                 + +YMW ETG  + KPLHG
Sbjct: 361 LGNTSAKRQAIFSFLIYSTTNRPS---TPGTKIEGGGEGPKEGLYMWRETGNSKGKPLHG 420

Query: 473 PFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSF 532
            FVA+LL+DLFG+Q+RGGC+CAGPYGH LL ID+  S  YR+AI KGY G+KPGWTR+SF
Sbjct: 421 AFVASLLNDLFGIQSRGGCACAGPYGHTLLKIDDTLSHHYRSAIQKGYLGMKPGWTRISF 480

Query: 533 PYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHI 592
           PYY+ N+EFEFIL ALEFIA YGQRFLPLY FNLR+GSWT K+K   DLL       N  
Sbjct: 481 PYYISNDEFEFILTALEFIAIYGQRFLPLYHFNLRSGSWTFKKKAHQDLLDNAIQGMNIG 540

Query: 593 FAFKNQCTNAEARLAAIVYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 650
                   NAE        K  +YLE+AK+IANLLPKFPP+R L EDI+ +LL FR+
Sbjct: 541 HDQSKDGNNAEKH--DQFAKFSAYLETAKRIANLLPKFPPQRRLPEDIDINLLPFRV 591

BLAST of MELO3C008050 vs. TrEMBL
Match: B9RU30_RICCO (Cysteine desulfurylase, putative OS=Ricinus communis GN=RCOM_1461070 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 8.1e-233
Identity = 410/619 (66.24%), Postives = 495/619 (79.97%), Query Frame = 1

Query: 32  TAESYGVCGRSRSFQTVEIDGVPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADH 91
           +A S  +C RS SF+T++I GVP S   E RLAWLRSQIIG + E +SPFG+RRL YADH
Sbjct: 39  SALSSDLCNRSESFRTLDI-GVPKSISSERRLAWLRSQIIGDDVEFESPFGKRRLTYADH 98

Query: 92  TASGRSLRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAIL 151
           TASGRSL YIE+FI+  VLPFYGNTHTCDSYVGH TTKMV++AT YIK+ LGGGEE+AI+
Sbjct: 99  TASGRSLHYIENFIINNVLPFYGNTHTCDSYVGHRTTKMVHEATQYIKRSLGGGEEDAII 158

Query: 152 FCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLA 211
           FCG GTT+A+KRLQEVMGIAVPSI+R+R+I+ L  EERWVVFVGPYEHHSNLLSWRQSLA
Sbjct: 159 FCGSGTTAAIKRLQEVMGIAVPSIMRDRIIKCLSNEERWVVFVGPYEHHSNLLSWRQSLA 218

Query: 212 EVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQY 271
           EVVEIG++++GL+D E L   L++Y+K  NRPILGSFSA SNVTGI+S T+ +A L+H+Y
Sbjct: 219 EVVEIGLNDSGLIDIEALEKLLDSYRKA-NRPILGSFSASSNVTGIFSKTRGLAQLLHRY 278

Query: 272 GGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSP 331
           GG VCFDFAASGPYV+IDM+ GE+DGYDAIFLS HKFLGGPGSPGILLM+K+LY+LK SP
Sbjct: 279 GGFVCFDFAASGPYVEIDMRYGEVDGYDAIFLSPHKFLGGPGSPGILLMSKALYQLKYSP 338

Query: 332 PSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEK 391
           PSTCGGGTV YVNGF +KDTLY ++IEERE GGTPQII  IRAALAFW+KEYIGY  IE+
Sbjct: 339 PSTCGGGTVCYVNGFNDKDTLYLDDIEERENGGTPQIIQTIRAALAFWVKEYIGYNVIEE 398

Query: 392 QEHQYIERALKRLLPNKSIGILGSTSSKRQAILSFIIYSSTNNTSPNCVTDMLCNPNSRE 451
           QE  YI +AL+R+  NK+I ILG+TS++RQAILSF+IYS+TN++S           +S++
Sbjct: 399 QEKNYISKALERVKSNKNIWILGNTSAERQAILSFLIYSTTNSSS----------SSSKD 458

Query: 452 KVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACSQA 511
           +  +     +TG  R KPLHG FVA+LL+DLFG+Q+RGGC+CAGPYGH LL+I EA S A
Sbjct: 459 ESSSS---SKTGNKRDKPLHGAFVASLLNDLFGIQSRGGCACAGPYGHVLLDISEANSLA 518

Query: 512 YRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTGSW 571
            R+AI +GY G KPGWTR+SFPYYM N+EFEFI+ A+EF+A YGQRFLPLY FN +TG+W
Sbjct: 519 LRSAIHQGYMGAKPGWTRISFPYYMSNQEFEFIMAAVEFVAIYGQRFLPLYGFNFKTGTW 578

Query: 572 TLKEKELADLL-GKENYSANHIFAFKNQCTNAEARLAAIVYKHKSYLESAKKIANLLPKF 631
             K+K   DLL GKEN         K       A    I+ K+ SYLE AK IANLLPKF
Sbjct: 579 YFKKKAFKDLLVGKEN---------KCNTFPMAAVGGEIINKYASYLEIAKHIANLLPKF 633

Query: 632 PPERELHEDIESSLLKFRI 650
           P  R+L ED++ +LL FR+
Sbjct: 639 PSSRKLPEDLDYNLLYFRV 633

BLAST of MELO3C008050 vs. NCBI nr
Match: gi|659080916|ref|XP_008441048.1| (PREDICTED: uncharacterized protein LOC103485275 [Cucumis melo])

HSP 1 Score: 1323.9 bits (3425), Expect = 0.0e+00
Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 1

Query: 1   MWKKPQSFAGEPYTSDRVYAEADVEDHAESGTAESYGVCGRSRSFQTVEIDGVPFSDKVE 60
           MWKKPQSFAGEPYTSDRVYAEADVEDHAESGTAESYGVCGRSRSFQTVEIDGVPFSDKVE
Sbjct: 35  MWKKPQSFAGEPYTSDRVYAEADVEDHAESGTAESYGVCGRSRSFQTVEIDGVPFSDKVE 94

Query: 61  ERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCD 120
           ERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCD
Sbjct: 95  ERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCD 154

Query: 121 SYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERV 180
           SYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERV
Sbjct: 155 SYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERV 214

Query: 181 IETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVG 240
           IETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVG
Sbjct: 215 IETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVG 274

Query: 241 NRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDA 300
           NRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDA
Sbjct: 275 NRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDA 334

Query: 301 IFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEER 360
           IFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEER
Sbjct: 335 IFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEER 394

Query: 361 ETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKR 420
           ETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKR
Sbjct: 395 ETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKR 454

Query: 421 QAILSFIIYSSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLS 480
           QAILSFIIYSSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLS
Sbjct: 455 QAILSFIIYSSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLS 514

Query: 481 DLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEE 540
           DLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEE
Sbjct: 515 DLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEE 574

Query: 541 FEFILKALEFIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCT 600
           FEFILKALEFIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCT
Sbjct: 575 FEFILKALEFIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCT 634

Query: 601 NAEARLAAIVYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 650
           NAEARLAAIVYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI
Sbjct: 635 NAEARLAAIVYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 683

BLAST of MELO3C008050 vs. NCBI nr
Match: gi|778717763|ref|XP_004148652.2| (PREDICTED: uncharacterized protein LOC101223213 [Cucumis sativus])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 604/649 (93.07%), Postives = 624/649 (96.15%), Query Frame = 1

Query: 1   MWKKPQSFAGEPYTSDRVYAEADVEDHAESGTAESYGVCGRSRSFQTVEIDGVPFSDKVE 60
           MW KPQSFAGE Y+SDRVYAEA     AES T ESYGVC RSRSF+TVE+DG+PFSD+VE
Sbjct: 35  MWVKPQSFAGEAYSSDRVYAEA-----AESATPESYGVCERSRSFRTVEMDGIPFSDRVE 94

Query: 61  ERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCD 120
           ER+ WLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCD
Sbjct: 95  ERMGWLRSQIIGGEAEIDSPFGERRLCYADHTASGRSLRYIEDFILRKVLPFYGNTHTCD 154

Query: 121 SYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERV 180
           SYVGHHTTKMV+DATTYIK+CLGGGEEEAILFCGQGTTSA+KRLQEVMGIAVPSILRERV
Sbjct: 155 SYVGHHTTKMVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERV 214

Query: 181 IETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVG 240
           IETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEA KK G
Sbjct: 215 IETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAG 274

Query: 241 NRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDA 300
           NRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDM+SGEIDGYDA
Sbjct: 275 NRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDA 334

Query: 301 IFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEER 360
           IFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLY ENIEER
Sbjct: 335 IFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEER 394

Query: 361 ETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKR 420
           E GGTPQIIGIIRAALAFW+KEYIGYQEIEKQEH+YIERALKRLLPN+SI ILGSTS+KR
Sbjct: 395 ENGGTPQIIGIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKR 454

Query: 421 QAILSFIIYSSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLS 480
           QAILSFIIYSSTNN+ PNC+TDML NPNSREKVK +YMWEETG MRAKPLHGPFVAALLS
Sbjct: 455 QAILSFIIYSSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLS 514

Query: 481 DLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEE 540
           DLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYM NEE
Sbjct: 515 DLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEE 574

Query: 541 FEFILKALEFIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCT 600
           FEFILKALEFIADYGQRFLPLYAFNLR GSWTLKEKEL DLLGKENYSA+HIFAFKNQCT
Sbjct: 575 FEFILKALEFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCT 634

Query: 601 NAEARLAAIVYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 650
           NAEA+LAAIVYKHKSYLESAK+IANLLPKFPPER LHEDIESSLL FRI
Sbjct: 635 NAEAKLAAIVYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 678

BLAST of MELO3C008050 vs. NCBI nr
Match: gi|700193156|gb|KGN48360.1| (hypothetical protein Csa_6G483410 [Cucumis sativus])

HSP 1 Score: 1003.4 bits (2593), Expect = 1.8e-289
Identity = 492/520 (94.62%), Postives = 506/520 (97.31%), Query Frame = 1

Query: 130 MVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEER 189
           MV+DATTYIK+CLGGGEEEAILFCGQGTTSA+KRLQEVMGIAVPSILRERVIETLKEEER
Sbjct: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60

Query: 190 WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFS 249
           WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEA KK GNRPILGSFS
Sbjct: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120

Query: 250 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFL 309
           ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDM+SGEIDGYDAIFLSTHKFL
Sbjct: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180

Query: 310 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQII 369
           GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLY ENIEERE GGTPQII
Sbjct: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240

Query: 370 GIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKRQAILSFIIY 429
           GIIRAALAFW+KEYIGYQEIEKQEH+YIERALKRLLPN+SI ILGSTS+KRQAILSFIIY
Sbjct: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300

Query: 430 SSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARG 489
           SSTNN+ PNC+TDML NPNSREKVK +YMWEETG MRAKPLHGPFVAALLSDLFGVQARG
Sbjct: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360

Query: 490 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALE 549
           GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYM NEEFEFILKALE
Sbjct: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420

Query: 550 FIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCTNAEARLAAI 609
           FIADYGQRFLPLYAFNLR GSWTLKEKEL DLLGKENYSA+HIFAFKNQCTNAEA+LAAI
Sbjct: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480

Query: 610 VYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 650
           VYKHKSYLESAK+IANLLPKFPPER LHEDIESSLL FRI
Sbjct: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 520

BLAST of MELO3C008050 vs. NCBI nr
Match: gi|703088096|ref|XP_010093446.1| (putative cysteine desulfurase [Morus notabilis])

HSP 1 Score: 854.7 bits (2207), Expect = 1.0e-244
Identity = 426/625 (68.16%), Postives = 512/625 (81.92%), Query Frame = 1

Query: 35  SYGVCGRSRSFQTVEIDGVPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADHTAS 94
           S G+C RS SF+++E+D VP S + + RL+WLRSQIIGG  E DSPFG+RRL YADHTAS
Sbjct: 68  SNGLCNRSDSFRSLEMD-VPKSCEDQVRLSWLRSQIIGGNVEYDSPFGKRRLTYADHTAS 127

Query: 95  GRSLRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCG 154
           GRSL YIE+FI+  VLPFYGNTHTCDS+VGH TTKMV++A  YIKKCLGGG+++A+ FCG
Sbjct: 128 GRSLLYIENFIIDNVLPFYGNTHTCDSHVGHRTTKMVHEAADYIKKCLGGGQDDALFFCG 187

Query: 155 QGTTSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVV 214
            GTT+A+KRLQEVMGIAVPSILRER ++ L  EE+WVVFVGP+EHHSNLLSWRQSLAEVV
Sbjct: 188 SGTTAAIKRLQEVMGIAVPSILRERAMKILSNEEKWVVFVGPFEHHSNLLSWRQSLAEVV 247

Query: 215 EIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGH 274
           EIG+DENGLLD E LR +LE+YK   +RPILGSFSA SNVTGI SDTKAIA L+HQYGG 
Sbjct: 248 EIGLDENGLLDMEALRLKLESYK-YAHRPILGSFSASSNVTGINSDTKAIAQLLHQYGGF 307

Query: 275 VCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPST 334
            CFDFAA GPY++IDM+SG++DGYDA+FLS HKFLGGPG+PGI+LM+K+LY+L+SS PST
Sbjct: 308 ACFDFAACGPYLEIDMRSGKMDGYDAVFLSPHKFLGGPGTPGIVLMSKALYRLRSSAPST 367

Query: 335 CGGGTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEH 394
           CGGGTV YVNGF+EKDTLY E+IEERE  GTPQII  IRAALAFW+KEY+GY+ IE+QE+
Sbjct: 368 CGGGTVAYVNGFSEKDTLYYEDIEERENAGTPQIIQTIRAALAFWVKEYVGYEVIERQEN 427

Query: 395 QYIERALKRLLPNKSIGILGSTSSKRQAILSFIIYSSTNNTS---PNCVTDMLCNPNSRE 454
            YI++A+KRLLPNKSI +LG+TS+KRQAILSF+IYS++N+ S    NC  D     N  E
Sbjct: 428 IYIQKAIKRLLPNKSIQVLGNTSAKRQAILSFLIYSTSNSYSRDLKNCCDDNYRTNNENE 487

Query: 455 KVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACSQA 514
           + + +YMW ETG  R KPLHGPFVAALL+DLFG+QARGGC+CAGPYGH LL +DE  S A
Sbjct: 488 E-EGLYMWRETGNSRDKPLHGPFVAALLNDLFGIQARGGCACAGPYGHALLKVDEPHSHA 547

Query: 515 YRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTGSW 574
           YR+AI KG+ G+KPGWTR+SFPYYM N+EFEFIL ALEFIA YGQRFL LY F+LRTG W
Sbjct: 548 YRSAIKKGFLGVKPGWTRISFPYYMANDEFEFILAALEFIATYGQRFLTLYDFSLRTGGW 607

Query: 575 TLKEKELADLLGKENYSANHIFAFK------NQCTNAEARLAAI-VYKHKSYLESAKKIA 634
           T ++K L  L+ K+N S N++ A K       Q  NAE +   I V K   +LE+AK IA
Sbjct: 608 TARKKALLCLV-KKNNSNNNVKAMKTENEVFKQSNNAETKHNGIMVNKFAMFLETAKDIA 667

Query: 635 NLLPKFPPERELHEDIESSLLKFRI 650
           +LLPKFPP+R L E+I+ + L FR+
Sbjct: 668 HLLPKFPPQRRLQEEIDLNFLHFRV 688

BLAST of MELO3C008050 vs. NCBI nr
Match: gi|590708692|ref|XP_007048349.1| (Chloroplastic NIFS-like cysteine desulfurase isoform 1 [Theobroma cacao])

HSP 1 Score: 846.7 bits (2186), Expect = 2.8e-242
Identity = 421/630 (66.83%), Postives = 508/630 (80.63%), Query Frame = 1

Query: 38  VCGRSRSFQTVEIDGVPFSDKVEERLAWLRSQIIGGEAEIDSPFGERRLCYADHTASGRS 97
           +C  S SF+T+E+ GVP  D  +++L+WLRSQIIGG+A+ D+PFG R+L YADHTASGR 
Sbjct: 44  LCHGSESFRTLEM-GVPKVDSTDKKLSWLRSQIIGGDAQFDTPFGTRKLTYADHTASGRC 103

Query: 98  LRYIEDFILRKVLPFYGNTHTCDSYVGHHTTKMVNDATTYIKKCLGGGEEEAILFCGQGT 157
           L YIE+FI+  VLPFYGNTHTCDSYVGH TTKMV++A+ Y+K+CLGGG+++AILFCG G+
Sbjct: 104 LHYIENFIVTNVLPFYGNTHTCDSYVGHRTTKMVHEASNYVKRCLGGGQDDAILFCGSGS 163

Query: 158 TSAVKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIG 217
           T+A+KRLQEVMGIAVPSILR+R++  L  E+RWVVFVGPYEHHSNLLSWRQSLAEVVEIG
Sbjct: 164 TAAIKRLQEVMGIAVPSILRDRLLNCLINEKRWVVFVGPYEHHSNLLSWRQSLAEVVEIG 223

Query: 218 MDENGLLDFEMLRSQLEAYKKVGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCF 277
           +DENGL++ E LR QLE YK   NRP+LGSFSACSNVTGI SDT+ IA L+H YGG VCF
Sbjct: 224 LDENGLINIEALRRQLELYKHA-NRPLLGSFSACSNVTGIQSDTREIAKLVHGYGGFVCF 283

Query: 278 DFAASGPYVQIDMQSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGG 337
           DFAASGPYV+IDM+SGE DGYDAIFLS HKFLGGPGSPGILLMNK+LY+L +SPPSTCGG
Sbjct: 284 DFAASGPYVEIDMRSGEFDGYDAIFLSPHKFLGGPGSPGILLMNKALYQLGASPPSTCGG 343

Query: 338 GTVTYVNGFTEKDTLYNENIEERETGGTPQIIGIIRAALAFWIKEYIGYQEIEKQEHQYI 397
           GTV YVNGF EKDTLY +++EERE  GTPQII IIRAAL FW+KEYIGY+ IEKQE  YI
Sbjct: 344 GTVNYVNGFNEKDTLYVDDVEERENAGTPQIIQIIRAALTFWVKEYIGYEVIEKQEKSYI 403

Query: 398 ERALKRLLPNKSIGILGSTS--SKRQAILSFIIYSSTN------NTSPNCVTDMLCNPNS 457
           ++AL+RLLPN++IGILG+TS  ++RQAILSF+++S+TN      N   N V ++    + 
Sbjct: 404 QQALERLLPNQNIGILGNTSTETERQAILSFLVFSTTNSCPGPVNKEGNHVDEL----DG 463

Query: 458 REKVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACS 517
           R K + ++MW ETG  R KPLHGPFV  LL+DLFG+QARGGC+CAGPYGH LL+IDE  S
Sbjct: 464 RMK-RGLFMWGETGNRRDKPLHGPFVVTLLNDLFGIQARGGCACAGPYGHSLLHIDEPRS 523

Query: 518 QAYRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALEFIADYGQRFLPLYAFNLRTG 577
            A+R AI KGY G KPGW RVSFPYYM NEEFEFIL A+EFIA YGQRFL LY FNLRTG
Sbjct: 524 LAFREAIEKGYAGAKPGWARVSFPYYMSNEEFEFILAAIEFIAIYGQRFLSLYHFNLRTG 583

Query: 578 SWTLKEKELADLLGKENYSANHIF----AF------KNQCTNAEARLAAIVYKHKSYLES 637
           SWT K+K L DL+GKE    +H+     AF      ++Q +  +    +++  + SYL+S
Sbjct: 584 SWTFKKKALKDLVGKEMSQGSHVLPLASAFPVTSVDRDQPSQYDDADVSVIRTYASYLKS 643

Query: 638 AKKIANLLPKFPPERELHEDIESSLLKFRI 650
           AK+IA LLPKFP +R LHEDI+  LL +R+
Sbjct: 644 AKRIAALLPKFPSQRRLHEDIDLDLLPYRL 666

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CSD_BACHD6.0e-1926.39Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM ... [more]
CSD_STRCO2.6e-1422.06Probable cysteine desulfurase OS=Streptomyces coelicolor (strain ATCC BAA-471 / ... [more]
CSD_CHLPN3.7e-1324.38Probable cysteine desulfurase OS=Chlamydia pneumoniae GN=csd PE=3 SV=1[more]
CSD_PYRAB8.3e-1329.91Probable cysteine desulfurase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=csd P... [more]
CSD_CHLMU6.6e-1031.14Probable cysteine desulfurase OS=Chlamydia muridarum (strain MoPn / Nigg) GN=csd... [more]
Match NameE-valueIdentityDescription
A0A0A0KF70_CUCSA1.2e-28994.62Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483410 PE=4 SV=1[more]
W9R1J9_9ROSA7.1e-24568.16Putative cysteine desulfurase OS=Morus notabilis GN=L484_017507 PE=4 SV=1[more]
A0A061DJH6_THECC1.9e-24266.83Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM... [more]
M5X0L3_PRUPE8.4e-23868.68Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003222mg PE=4 SV=1[more]
B9RU30_RICCO8.1e-23366.24Cysteine desulfurylase, putative OS=Ricinus communis GN=RCOM_1461070 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
gi|659080916|ref|XP_008441048.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC103485275 [Cucumis melo][more]
gi|778717763|ref|XP_004148652.2|0.0e+0093.07PREDICTED: uncharacterized protein LOC101223213 [Cucumis sativus][more]
gi|700193156|gb|KGN48360.1|1.8e-28994.62hypothetical protein Csa_6G483410 [Cucumis sativus][more]
gi|703088096|ref|XP_010093446.1|1.0e-24468.16putative cysteine desulfurase [Morus notabilis][more]
gi|590708692|ref|XP_007048349.1|2.8e-24266.83Chloroplastic NIFS-like cysteine desulfurase isoform 1 [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000192Aminotrans_V_dom
IPR015421PyrdxlP-dep_Trfase_major
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015424PyrdxlP-dep_Trfase
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0031071 cysteine desulfurase activity
molecular_function GO:0008483 transaminase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU62696melon EST collection version 4.0transcribed_cluster
MU64347melon EST collection version 4.0transcribed_cluster
MU67424melon EST collection version 4.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C008050T1MELO3C008050T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU62696MU62696transcribed_cluster
MU67424MU67424transcribed_cluster
MU64347MU64347transcribed_cluster


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 112..428
score: 3.3
IPR015421Pyridoxal phosphate-dependent transferase, major region, subdomain 1GENE3DG3DSA:3.40.640.10coord: 100..366
score: 8.4
IPR015422Pyridoxal phosphate-dependent transferase, major region, subdomain 2GENE3DG3DSA:3.90.1150.10coord: 484..556
score: 2.9E-16coord: 367..447
score: 2.9
IPR015424Pyridoxal phosphate-dependent transferaseunknownSSF53383PLP-dependent transferasescoord: 80..430
score: 5.59E-62coord: 470..549
score: 5.59
NoneNo IPR availablePANTHERPTHR11601CYSTEINE DESULFURYLASEcoord: 41..427
score: 4.3E-291coord: 467..587
score: 4.3E
NoneNo IPR availablePANTHERPTHR11601:SF24SUBFAMILY NOT NAMEDcoord: 41..427
score: 4.3E-291coord: 467..587
score: 4.3E