Csa6G483410 (gene) Cucumber (Chinese Long) v2

NameCsa6G483410
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCysteine desulfurase; contains IPR015424 (Pyridoxal phosphate-dependent transferase)
LocationChr6 : 22246570 .. 22248412 (-)
   



The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCACGATGCAACTACGTACATCAAGAGATGCTTAGGAGGTGGAGAAGAAGAAGCAATTCTATTCTGTGGACAAGGCACAACTTCCGCCATTAAAAGACTTCAAGAAGTGATGGGCATTGCAGTGCCATCCATTCTAAGAGAGAGGGTAATAGAAACCCTAAAGGAAGAAGAAAGATGGGTTGTTTTTGTTGGACCTTATGAGCATCACTCCAACCTCCTCTCGTGGCGGCAGAGCTTAGCCGAGGTAGTTGAAATTGGAATGGATGAGAATGGGCTTTTAGACTTCGAAATGCTCAGATCCCAACTTGAAGCATGTAAGAAAGCTGGAAATAGACCAATTTTGGGTTCTTTTTCAGCTTGTAGTAATGTCACAGGAATTTATTCAGACACAAAAGCTATTGCTACACTAATTCATCAATATGGAGGCCATGTTTGCTTTGATTTTGCTGCAAGGTACATGAAATTTTACCTATTTTCTAGAGTTAAAGCATTCACTAAATATATGATAATTGAATCTATTTGTCTATGGTGTGTAGTGGTCCTTATGTGCAAATTGATATGAAGTCGGGGGAAATTGATGGCTATGATGCCATTTTTTTAAGTACGCACAAGTTTCTTGGAGGGCCTGGATCACCTGGAATCCTTCTGATGAACAAAAGTCTCTACAAGTTAAAATCATCTCCTCCATCAACTTGTGGGGGTGGTACTGTGACTTATGTCAATGGCTTCACTGAAAAGGTAAATGGAAAGCTTACCAAATTGAGTTTTGTTCGAATGGAAGCCAATAGATTGAAGCTGAAATTTTGATATATTTTGACGTTTGTTCCTTTGCTCGTGGAAAACTTTCTCCAGGACACACTGTATTACGAAAACATAGAAGAGAGGGAAAATGGTGGAACGCCACAGATTATAGGAATAATAAGAGCAGCTTTGGCTTTTTGGGTAAAAGAATATATCGGCTATCAAGAGATAGAGAAGCAAGAGCATCGGTATATAGAAAGGGCTTTGAAGAGACTTCTCCCAAATAGAAGCATCAGTATTTTAGGGAGCACGTCTACAAAGCGACAGGCGATATTATCTTTTATCATTTACTCCTCGACCAATAATTCATTGCCCAACTGTATCACAGATATGTTAGGTAATCCTAACAGCAGAGAAAAGGTTAAAAAGCTTTACATGTGGGAAGAAACGGGGAGTATGAGAGCCAAACCTCTTCACGGTCCTTTTGTAGCAGCGCTCCTGAGCGATTTGTTTGGAGTTCAGGCTCGAGGGGGATGCAGTTGTGCTGGTCCTTATGGTCACAAGCTGCTCAACATTGATGAAGCATGCTCGCAAGCCTACAGAACTGCCATTGCTAAGGTATTAATCAGTTTGATTTTTCAACCATGAAATAGTGAGATTCGTTCCATCATCTTTCAACTTTATCTTTAATAATCTAATAGGGCTATGAGGGAATAAAACCTGGATGGACAAGAGTCAGCTTTCCGTACTACATGTCAAATGAGGAGTTCGAATTCATTTTAAAAGCTTTAGAATTCATAGCTGATTATGGACAAAGATTCCTTCCTTTGTATGCCTTCAATTTGAGAAATGGTAGCTGGACACTGAAGGAAAAGGAGCTCACCGACCTACTCGGGAAGGAGAATTACAGTGCCAGTCACATCTTTGCATTCAAAAACCAGTGTACCAATGCAGAAGCTAAGTTAGCAGCCATAGTATACAAACATAAATCATATTTAGAATCTGCTAAAAGAATCGCCAACCTTCTCCCCAAGTTCCCTCCTGAGAGAGTGCTTCACGAGGATATTGAATCCTCTCTATTAACATTCAGAATCTAG

mRNA sequence

ATGGTGCACGATGCAACTACGTACATCAAGAGATGCTTAGGAGGTGGAGAAGAAGAAGCAATTCTATTCTGTGGACAAGGCACAACTTCCGCCATTAAAAGACTTCAAGAAGTGATGGGCATTGCAGTGCCATCCATTCTAAGAGAGAGGGTAATAGAAACCCTAAAGGAAGAAGAAAGATGGGTTGTTTTTGTTGGACCTTATGAGCATCACTCCAACCTCCTCTCGTGGCGGCAGAGCTTAGCCGAGGTAGTTGAAATTGGAATGGATGAGAATGGGCTTTTAGACTTCGAAATGCTCAGATCCCAACTTGAAGCATGTAAGAAAGCTGGAAATAGACCAATTTTGGGTTCTTTTTCAGCTTGTAGTAATGTCACAGGAATTTATTCAGACACAAAAGCTATTGCTACACTAATTCATCAATATGGAGGCCATGTTTGCTTTGATTTTGCTGCAAGTGGTCCTTATGTGCAAATTGATATGAAGTCGGGGGAAATTGATGGCTATGATGCCATTTTTTTAAGTACGCACAAGTTTCTTGGAGGGCCTGGATCACCTGGAATCCTTCTGATGAACAAAAGTCTCTACAAGTTAAAATCATCTCCTCCATCAACTTGTGGGGGTGGTACTGTGACTTATGTCAATGGCTTCACTGAAAAGGACACACTGTATTACGAAAACATAGAAGAGAGGGAAAATGGTGGAACGCCACAGATTATAGGAATAATAAGAGCAGCTTTGGCTTTTTGGGTAAAAGAATATATCGGCTATCAAGAGATAGAGAAGCAAGAGCATCGGTATATAGAAAGGGCTTTGAAGAGACTTCTCCCAAATAGAAGCATCAGTATTTTAGGGAGCACGTCTACAAAGCGACAGGCGATATTATCTTTTATCATTTACTCCTCGACCAATAATTCATTGCCCAACTGTATCACAGATATGTTAGGTAATCCTAACAGCAGAGAAAAGGTTAAAAAGCTTTACATGTGGGAAGAAACGGGGAGTATGAGAGCCAAACCTCTTCACGGTCCTTTTGTAGCAGCGCTCCTGAGCGATTTGTTTGGAGTTCAGGCTCGAGGGGGATGCAGTTGTGCTGGTCCTTATGGTCACAAGCTGCTCAACATTGATGAAGCATGCTCGCAAGCCTACAGAACTGCCATTGCTAAGGGCTATGAGGGAATAAAACCTGGATGGACAAGAGTCAGCTTTCCGTACTACATGTCAAATGAGGAGTTCGAATTCATTTTAAAAGCTTTAGAATTCATAGCTGATTATGGACAAAGATTCCTTCCTTTGTATGCCTTCAATTTGAGAAATGGTAGCTGGACACTGAAGGAAAAGGAGCTCACCGACCTACTCGGGAAGGAGAATTACAGTGCCAGTCACATCTTTGCATTCAAAAACCAGTGTACCAATGCAGAAGCTAAGTTAGCAGCCATAGTATACAAACATAAATCATATTTAGAATCTGCTAAAAGAATCGCCAACCTTCTCCCCAAGTTCCCTCCTGAGAGAGTGCTTCACGAGGATATTGAATCCTCTCTATTAACATTCAGAATCTAG

Coding sequence (CDS)

ATGGTGCACGATGCAACTACGTACATCAAGAGATGCTTAGGAGGTGGAGAAGAAGAAGCAATTCTATTCTGTGGACAAGGCACAACTTCCGCCATTAAAAGACTTCAAGAAGTGATGGGCATTGCAGTGCCATCCATTCTAAGAGAGAGGGTAATAGAAACCCTAAAGGAAGAAGAAAGATGGGTTGTTTTTGTTGGACCTTATGAGCATCACTCCAACCTCCTCTCGTGGCGGCAGAGCTTAGCCGAGGTAGTTGAAATTGGAATGGATGAGAATGGGCTTTTAGACTTCGAAATGCTCAGATCCCAACTTGAAGCATGTAAGAAAGCTGGAAATAGACCAATTTTGGGTTCTTTTTCAGCTTGTAGTAATGTCACAGGAATTTATTCAGACACAAAAGCTATTGCTACACTAATTCATCAATATGGAGGCCATGTTTGCTTTGATTTTGCTGCAAGTGGTCCTTATGTGCAAATTGATATGAAGTCGGGGGAAATTGATGGCTATGATGCCATTTTTTTAAGTACGCACAAGTTTCTTGGAGGGCCTGGATCACCTGGAATCCTTCTGATGAACAAAAGTCTCTACAAGTTAAAATCATCTCCTCCATCAACTTGTGGGGGTGGTACTGTGACTTATGTCAATGGCTTCACTGAAAAGGACACACTGTATTACGAAAACATAGAAGAGAGGGAAAATGGTGGAACGCCACAGATTATAGGAATAATAAGAGCAGCTTTGGCTTTTTGGGTAAAAGAATATATCGGCTATCAAGAGATAGAGAAGCAAGAGCATCGGTATATAGAAAGGGCTTTGAAGAGACTTCTCCCAAATAGAAGCATCAGTATTTTAGGGAGCACGTCTACAAAGCGACAGGCGATATTATCTTTTATCATTTACTCCTCGACCAATAATTCATTGCCCAACTGTATCACAGATATGTTAGGTAATCCTAACAGCAGAGAAAAGGTTAAAAAGCTTTACATGTGGGAAGAAACGGGGAGTATGAGAGCCAAACCTCTTCACGGTCCTTTTGTAGCAGCGCTCCTGAGCGATTTGTTTGGAGTTCAGGCTCGAGGGGGATGCAGTTGTGCTGGTCCTTATGGTCACAAGCTGCTCAACATTGATGAAGCATGCTCGCAAGCCTACAGAACTGCCATTGCTAAGGGCTATGAGGGAATAAAACCTGGATGGACAAGAGTCAGCTTTCCGTACTACATGTCAAATGAGGAGTTCGAATTCATTTTAAAAGCTTTAGAATTCATAGCTGATTATGGACAAAGATTCCTTCCTTTGTATGCCTTCAATTTGAGAAATGGTAGCTGGACACTGAAGGAAAAGGAGCTCACCGACCTACTCGGGAAGGAGAATTACAGTGCCAGTCACATCTTTGCATTCAAAAACCAGTGTACCAATGCAGAAGCTAAGTTAGCAGCCATAGTATACAAACATAAATCATATTTAGAATCTGCTAAAAGAATCGCCAACCTTCTCCCCAAGTTCCCTCCTGAGAGAGTGCTTCACGAGGATATTGAATCCTCTCTATTAACATTCAGAATCTAG

Protein sequence

MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQIIGIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIYSSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALEFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAIVYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI*
BLAST of Csa6G483410 vs. Swiss-Prot
Match: CSD_BACHD (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=csd PE=3 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 1.0e-16
Identity = 82/295 (27.80%), Postives = 130/295 (44.07%), Query Frame = 1

Query: 9   IKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEERWVVFVGPY 68
           I+R +     E I+F  +GTT+AI  +    G A            L E +   + + P 
Sbjct: 74  IRRFIHASSTEEIIFT-RGTTTAINLVAASYGRA-----------NLGEGDE--IVITPM 133

Query: 69  EHHSNLLSWRQSL----AEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFSACSN 128
           EHHSN++ W+Q      A +  + + ++G +  E +   +    K      + +    SN
Sbjct: 134 EHHSNIIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTK------IVAIMHVSN 193

Query: 129 VTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFLGGPG 188
           V G  +  K IA + H++G  +  D A S P+++ID++     G D    S HK + GP 
Sbjct: 194 VLGTINPVKEIAEIAHRHGAIMLVDGAQSAPHMKIDVQEL---GCDFFAFSGHK-MAGPT 253

Query: 189 SPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQIIGIIR 248
             G+L   K+   L+   P   GG  + +V  +       ++ +  +  GGTP I G I 
Sbjct: 254 GIGVLYGKKA--HLEKMEPVEFGGEMIDFVGLYDST----WKELPWKFEGGTPIIAGAIG 313

Query: 249 AALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFII 300
              A    E IG  EIEK EH   + AL RL     +++ G    KR  +++F I
Sbjct: 314 LGAAIDFLEDIGLDEIEKHEHELAQYALDRLSELEGMTVYG--PQKRAGLVTFNI 336

BLAST of Csa6G483410 vs. Swiss-Prot
Match: CSD_PYRAB (Probable cysteine desulfurase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=csd PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-13
Identity = 65/214 (30.37%), Postives = 96/214 (44.86%), Query Frame = 1

Query: 67  PYEHHSNLLSWRQSLAEVVEIGM-----DENGLLDFEMLRSQLEACKKAGNRPILGSFSA 126
           PYEHHSNLL W Q LA+   + +     D+ G LD      +++  K       L +   
Sbjct: 111 PYEHHSNLLPW-QRLAKKKGLKLEFIEGDDEGNLDLADAEKKIKGAK-------LVAVQH 170

Query: 127 CSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFLG 186
            SN  G+  + + +  ++ + G     D A S  ++++D+K  + D    +  S HK   
Sbjct: 171 VSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGHMEVDVKKLKAD---FLAFSGHKGPM 230

Query: 187 GPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQIIG 246
           GP   G+L +NK  + +   P    GGGT+  V     K T       ER   GTP I G
Sbjct: 231 GPTGIGVLYINKEFFDVFEPP--LIGGGTIEDVELCCYKLT----EPPERFEAGTPNIGG 290

Query: 247 IIRAALAFWVKEYIGYQEIEKQEHRYIERALKRL 276
            I  A      E IG  +IEKQE + ++R  + L
Sbjct: 291 AIGLAAGIKYIEKIGIDKIEKQERKLVKRTTEGL 307

BLAST of Csa6G483410 vs. Swiss-Prot
Match: CSD_CHLPN (Probable cysteine desulfurase OS=Chlamydia pneumoniae GN=csd PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.5e-12
Identity = 69/243 (28.40%), Postives = 106/243 (43.62%), Query Frame = 1

Query: 62  VVFVGPYEHHSNLLSW----RQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILG 121
           VV V   EHH+N+LSW    R+  + V +I + ++GL+D + L   L    +  + P + 
Sbjct: 112 VVLVSEAEHHANVLSWEIACRRRGSLVKKIRVHDSGLIDLDDLEKLLNEGAQFVSIPHV- 171

Query: 122 SFSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTH 181
                SNVTG     + +A L+H+Y  ++  D A   P++ ID++  ++D Y     S+H
Sbjct: 172 -----SNVTGCVQPLQQVAELVHRYDAYLAVDGAQGAPHLPIDVQLWDVDFY---VFSSH 231

Query: 182 KFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTP 241
           K + GP   G+L   K L  L   PP   GG  V       +     Y     +   GTP
Sbjct: 232 K-IYGPTGIGVLYGKKDL--LDQLPPVEGGGDMV----AIYDHQNPEYLPAPMKFEAGTP 291

Query: 242 QIIGIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILG-STSTKRQAILS 300
            I G++    A    + +  + I  +E        K LL    + ILG S    R A++ 
Sbjct: 292 NIAGVLGLGAALDYLDGLSAKFIYDKEIALTTYLHKELLEIPGVEILGPSIEEPRGALIG 338

BLAST of Csa6G483410 vs. Swiss-Prot
Match: CSD_STRCO (Probable cysteine desulfurase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) GN=csd PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 7.4e-12
Identity = 62/242 (25.62%), Postives = 108/242 (44.63%), Query Frame = 1

Query: 63  VFVGPYEHHSNLLSWR----QSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGS 122
           + +   EHHSN++ W+    ++ A++   G+ ++G LD   +   +    K      + S
Sbjct: 126 IVITEMEHHSNIVPWQLLAQRTGAKLRWFGLTDDGRLDLSNIDEVITEKTK------VVS 185

Query: 123 FSACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHK 182
           F   SN+ G  +  +AI     + G  VC D + + P++ +D+++ + D    +  + HK
Sbjct: 186 FVLVSNILGTQNPVEAIVRRAQEVGALVCIDASQAAPHMPLDVQALQAD---FVAFTGHK 245

Query: 183 FLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQ 242
             G P   G+L   + L  L+  PP   GG  +  V+  +      Y     +   GTP 
Sbjct: 246 MCG-PTGIGVLWGRQEL--LEDLPPFLGGGEMIETVSMHSST----YAPAPHKFEAGTPP 305

Query: 243 IIGIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILG-STSTKRQAILSF 300
           +   +    A      IG  +I   EH   E A+KRLL    + I+G +T+ +R A +SF
Sbjct: 306 VAQAVGLGAAIDYLNSIGMDKILAHEHALTEYAVKRLLEVPDLRIIGPTTAEERGAAISF 351

BLAST of Csa6G483410 vs. Swiss-Prot
Match: CSD_CHLMU (Probable cysteine desulfurase OS=Chlamydia muridarum (strain MoPn / Nigg) GN=csd PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-11
Identity = 77/250 (30.80%), Postives = 112/250 (44.80%), Query Frame = 1

Query: 63  VFVGPYEHHSNLLSW----RQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGS 122
           V V   EHH+N+LSW    R+S A V ++ +++ G++D     S LE   K G +  L S
Sbjct: 109 VVVSEAEHHANILSWEIACRRSGATVKKVRVNDEGIIDL----SHLEKLLKQGVQ--LVS 168

Query: 123 FSACSNVTGIYSDTKAIATLIHQYGGHVCFDFA---ASGPYVQIDMKSGEIDGYDAIFLS 182
            +  SNV+G     + +A L+H+YG  +  D A    SGP   +++   ++D Y     S
Sbjct: 169 LAHVSNVSGAVLPVQEVAFLVHRYGALLAIDGAQGVGSGP---LNLSGWDVDFY---AFS 228

Query: 183 THKFLGGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGG 242
            HK L  P   G+L   + L  L+S PP   GG  V       + ++  Y+    R   G
Sbjct: 229 GHK-LYAPTGIGVLYGKREL--LESLPPVEGGGDMVVVY----DSESSRYQEPPLRFEAG 288

Query: 243 TPQIIGIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNR-----SISILG-STST 300
           TP I G++    A    +Y+        +H     AL R L NR      I I+G    T
Sbjct: 289 TPHIAGVLGLGAAI---DYLQALPFSVSDHL---TALTRFLYNRLLTIPDIQIVGPQQGT 333

BLAST of Csa6G483410 vs. TrEMBL
Match: A0A0A0KF70_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483410 PE=4 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 1.1e-304
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER
Sbjct: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS
Sbjct: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL
Sbjct: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII
Sbjct: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
           GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY
Sbjct: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300

Query: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360
           SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG
Sbjct: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360

Query: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420
           GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE
Sbjct: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420

Query: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480
           FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI
Sbjct: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480

Query: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
           VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI
Sbjct: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 520

BLAST of Csa6G483410 vs. TrEMBL
Match: W9R1J9_9ROSA (Putative cysteine desulfurase OS=Morus notabilis GN=L484_017507 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 3.0e-206
Identity = 362/530 (68.30%), Postives = 435/530 (82.08%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVH+A  YIK+CLGGG+++A+ FCG GTT+AIKRLQEVMGIAVPSILRER ++ L  EE+
Sbjct: 162 MVHEAADYIKKCLGGGQDDALFFCGSGTTAAIKRLQEVMGIAVPSILRERAMKILSNEEK 221

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGP+EHHSNLLSWRQSLAEVVEIG+DENGLLD E LR +LE+ K A +RPILGSFS
Sbjct: 222 WVVFVGPFEHHSNLLSWRQSLAEVVEIGLDENGLLDMEALRLKLESYKYA-HRPILGSFS 281

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           A SNVTGI SDTKAIA L+HQYGG  CFDFAA GPY++IDM+SG++DGYDA+FLS HKFL
Sbjct: 282 ASSNVTGINSDTKAIAQLLHQYGGFACFDFAACGPYLEIDMRSGKMDGYDAVFLSPHKFL 341

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPG+PGI+LM+K+LY+L+SS PSTCGGGTV YVNGF+EKDTLYYE+IEEREN GTPQII
Sbjct: 342 GGPGTPGIVLMSKALYRLRSSAPSTCGGGTVAYVNGFSEKDTLYYEDIEERENAGTPQII 401

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
             IRAALAFWVKEY+GY+ IE+QE+ YI++A+KRLLPN+SI +LG+TS KRQAILSF+IY
Sbjct: 402 QTIRAALAFWVKEYVGYEVIERQENIYIQKAIKRLLPNKSIQVLGNTSAKRQAILSFLIY 461

Query: 301 SSTNN---SLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQ 360
           S++N+    L NC  D     N  E+ + LYMW ETG+ R KPLHGPFVAALL+DLFG+Q
Sbjct: 462 STSNSYSRDLKNCCDDNYRTNNENEE-EGLYMWRETGNSRDKPLHGPFVAALLNDLFGIQ 521

Query: 361 ARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILK 420
           ARGGC+CAGPYGH LL +DE  S AYR+AI KG+ G+KPGWTR+SFPYYM+N+EFEFIL 
Sbjct: 522 ARGGCACAGPYGHALLKVDEPHSHAYRSAIKKGFLGVKPGWTRISFPYYMANDEFEFILA 581

Query: 421 ALEFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFK------NQCT 480
           ALEFIA YGQRFL LY F+LR G WT ++K L  L+ K+N S +++ A K       Q  
Sbjct: 582 ALEFIATYGQRFLTLYDFSLRTGGWTARKKALLCLV-KKNNSNNNVKAMKTENEVFKQSN 641

Query: 481 NAEAKLAAI-VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
           NAE K   I V K   +LE+AK IA+LLPKFPP+R L E+I+ + L FR+
Sbjct: 642 NAETKHNGIMVNKFAMFLETAKDIAHLLPKFPPQRRLQEEIDLNFLHFRV 688

BLAST of Csa6G483410 vs. TrEMBL
Match: A0A061DJH6_THECC (Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM_001454 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.2e-205
Identity = 366/535 (68.41%), Postives = 433/535 (80.93%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVH+A+ Y+KRCLGGG+++AILFCG G+T+AIKRLQEVMGIAVPSILR+R++  L  E+R
Sbjct: 135 MVHEASNYVKRCLGGGQDDAILFCGSGSTAAIKRLQEVMGIAVPSILRDRLLNCLINEKR 194

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIG+DENGL++ E LR QLE  K A NRP+LGSFS
Sbjct: 195 WVVFVGPYEHHSNLLSWRQSLAEVVEIGLDENGLINIEALRRQLELYKHA-NRPLLGSFS 254

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGI SDT+ IA L+H YGG VCFDFAASGPYV+IDM+SGE DGYDAIFLS HKFL
Sbjct: 255 ACSNVTGIQSDTREIAKLVHGYGGFVCFDFAASGPYVEIDMRSGEFDGYDAIFLSPHKFL 314

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGILLMNK+LY+L +SPPSTCGGGTV YVNGF EKDTLY +++EEREN GTPQII
Sbjct: 315 GGPGSPGILLMNKALYQLGASPPSTCGGGTVNYVNGFNEKDTLYVDDVEERENAGTPQII 374

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTS--TKRQAILSFI 300
            IIRAAL FWVKEYIGY+ IEKQE  YI++AL+RLLPN++I ILG+TS  T+RQAILSF+
Sbjct: 375 QIIRAALTFWVKEYIGYEVIEKQEKSYIQQALERLLPNQNIGILGNTSTETERQAILSFL 434

Query: 301 IYSSTNNSLPNCIT---DMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFG 360
           ++S+T NS P  +    + +   + R K + L+MW ETG+ R KPLHGPFV  LL+DLFG
Sbjct: 435 VFSTT-NSCPGPVNKEGNHVDELDGRMK-RGLFMWGETGNRRDKPLHGPFVVTLLNDLFG 494

Query: 361 VQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFI 420
           +QARGGC+CAGPYGH LL+IDE  S A+R AI KGY G KPGW RVSFPYYMSNEEFEFI
Sbjct: 495 IQARGGCACAGPYGHSLLHIDEPRSLAFREAIEKGYAGAKPGWARVSFPYYMSNEEFEFI 554

Query: 421 LKALEFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIF----AF----- 480
           L A+EFIA YGQRFL LY FNLR GSWT K+K L DL+GKE    SH+     AF     
Sbjct: 555 LAAIEFIAIYGQRFLSLYHFNLRTGSWTFKKKALKDLVGKEMSQGSHVLPLASAFPVTSV 614

Query: 481 -KNQCTNAEAKLAAIVYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
            ++Q +  +    +++  + SYL+SAKRIA LLPKFP +R LHEDI+  LL +R+
Sbjct: 615 DRDQPSQYDDADVSVIRTYASYLKSAKRIAALLPKFPSQRRLHEDIDLDLLPYRL 666

BLAST of Csa6G483410 vs. TrEMBL
Match: M5X0L3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003222mg PE=4 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 1.1e-203
Identity = 359/521 (68.91%), Postives = 421/521 (80.81%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVH+A  YIK+CLGGG EEA+ FCGQGTTSAIKRLQEVMGIAVPSILR+RVI  L  EER
Sbjct: 78  MVHEAAKYIKKCLGGGPEEALFFCGQGTTSAIKRLQEVMGIAVPSILRDRVINNLNTEER 137

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           W+VFVGPYEHHSNLLSWRQSLAEVVEIG++++GLLD E LR QLE  K   NRPILGSFS
Sbjct: 138 WLVFVGPYEHHSNLLSWRQSLAEVVEIGVNDDGLLDLEALRLQLEKYKYV-NRPILGSFS 197

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGIYSDT+AIA LIH+YGG  CFDFAASGPYV+I+M+ GE+DGYD +FLS HKFL
Sbjct: 198 ACSNVTGIYSDTRAIARLIHRYGGFACFDFAASGPYVEIEMRCGEMDGYDGVFLSPHKFL 257

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGIL+M+K+LY+L+S PPSTCGGGTV YVNGF+++DTLY+E++EERENGGTPQII
Sbjct: 258 GGPGSPGILIMSKALYQLRSLPPSTCGGGTVAYVNGFSDEDTLYWEDVEERENGGTPQII 317

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
             IRAALAFWVKEYIGYQ IEK E  Y++RAL+RLLPN ++ +LG+TS KRQAI SF+IY
Sbjct: 318 QTIRAALAFWVKEYIGYQVIEKHEDFYVKRALERLLPNNNVWVLGNTSAKRQAIFSFLIY 377

Query: 301 SSTNN-SLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQAR 360
           S+TN  S P   T + G     +  + LYMW ETG+ + KPLHG FVA+LL+DLFG+Q+R
Sbjct: 378 STTNRPSTPG--TKIEGGGEGPK--EGLYMWRETGNSKGKPLHGAFVASLLNDLFGIQSR 437

Query: 361 GGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKAL 420
           GGC+CAGPYGH LL ID+  S  YR+AI KGY G+KPGWTR+SFPYY+SN+EFEFIL AL
Sbjct: 438 GGCACAGPYGHTLLKIDDTLSHHYRSAIQKGYLGMKPGWTRISFPYYISNDEFEFILTAL 497

Query: 421 EFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAA 480
           EFIA YGQRFLPLY FNLR+GSWT K+K   DLL  +N                 A+   
Sbjct: 498 EFIAIYGQRFLPLYHFNLRSGSWTFKKKAHQDLL--DNAIQGMNIGHDQSKDGNNAEKHD 557

Query: 481 IVYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
              K  +YLE+AKRIANLLPKFPP+R L EDI+ +LL FR+
Sbjct: 558 QFAKFSAYLETAKRIANLLPKFPPQRRLPEDIDINLLPFRV 591

BLAST of Csa6G483410 vs. TrEMBL
Match: F6I0F5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0044g01150 PE=4 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 1.2e-199
Identity = 350/533 (65.67%), Postives = 424/533 (79.55%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVH+AT Y+K+CLGG +++A++FCG GTT+AIKRLQEVMGI  PSI+RERV++TL+ EER
Sbjct: 133 MVHEATKYVKKCLGGKQDDALVFCGSGTTAAIKRLQEVMGITSPSIMRERVLKTLRSEER 192

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIG+DENGL+D + L+ QLE+ K   NRP+LGSFS
Sbjct: 193 WVVFVGPYEHHSNLLSWRQSLAEVVEIGLDENGLIDMKALKQQLESYKYT-NRPLLGSFS 252

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGIY++T+A+A ++HQ+G  VCFDFAASGPYV+IDM+SGEIDGYDAIFLS HKFL
Sbjct: 253 ACSNVTGIYTNTRALAKILHQHGAFVCFDFAASGPYVEIDMRSGEIDGYDAIFLSPHKFL 312

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPG+PGILLM+K LY L SSPPSTCGGGTV +VNGF EKDTLY + IEERE+ GTP I+
Sbjct: 313 GGPGTPGILLMSKVLYNLASSPPSTCGGGTVAFVNGFNEKDTLYVDEIEEREDAGTPPIV 372

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
             I+ AL FWVKEYIGY  IE+ EH YIE AL+RLLPN +I +LG+TS KRQAILSF++Y
Sbjct: 373 QKIKTALTFWVKEYIGYNVIEQMEHMYIEAALERLLPNPNIKVLGNTSAKRQAILSFLVY 432

Query: 301 SSTNNSLPNCITDMLGNPNSREKVK-KLYMWEETGSMRAKPLHGPFVAALLSDLFGVQAR 360
           S+TN+S         G  +SR  ++ + YMW ETG+ R KPLHGPFVA LL+DLFG+QAR
Sbjct: 433 STTNSSSD-------GMKDSRGDIEGRFYMWRETGNKRGKPLHGPFVAKLLNDLFGIQAR 492

Query: 361 GGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKAL 420
           GGC+CAGPYGH LLN+DE  S A+R+AI  G  G+KPGWTR+SFPYYMS EEFEFIL AL
Sbjct: 493 GGCACAGPYGHSLLNVDETRSLAFRSAIHNGNNGVKPGWTRISFPYYMSIEEFEFILAAL 552

Query: 421 EFIADYGQRFLPLYAFNLRNGSWTLKEKELTD-LLGKENYS-----------ASHIFAFK 480
           EFIA YGQRFLPLY FN + G+WT K+K L D L GKEN              + I + +
Sbjct: 553 EFIATYGQRFLPLYNFNWKTGNWTFKKKALKDALAGKENNGNFGGLSLARGMQASIMSNQ 612

Query: 481 NQCTNAEA-KLAAIVYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFR 520
           ++  +  A +   I+ K+ SYLE+AKRIA+LL KFPP R + EDI+ +L TFR
Sbjct: 613 SEAQHNNADENDEIINKYTSYLETAKRIASLLEKFPPHRRVPEDIDLNLCTFR 657

BLAST of Csa6G483410 vs. NCBI nr
Match: gi|778717763|ref|XP_004148652.2| (PREDICTED: uncharacterized protein LOC101223213 [Cucumis sativus])

HSP 1 Score: 1053.1 bits (2722), Expect = 1.6e-304
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER
Sbjct: 159 MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 218

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS
Sbjct: 219 WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 278

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL
Sbjct: 279 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 338

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII
Sbjct: 339 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 398

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
           GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY
Sbjct: 399 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 458

Query: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360
           SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG
Sbjct: 459 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 518

Query: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420
           GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE
Sbjct: 519 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 578

Query: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480
           FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI
Sbjct: 579 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 638

Query: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
           VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI
Sbjct: 639 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 678

BLAST of Csa6G483410 vs. NCBI nr
Match: gi|700193156|gb|KGN48360.1| (hypothetical protein Csa_6G483410 [Cucumis sativus])

HSP 1 Score: 1053.1 bits (2722), Expect = 1.6e-304
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER
Sbjct: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS
Sbjct: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL
Sbjct: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII
Sbjct: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
           GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY
Sbjct: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300

Query: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360
           SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG
Sbjct: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360

Query: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420
           GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE
Sbjct: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420

Query: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480
           FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI
Sbjct: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480

Query: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
           VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI
Sbjct: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 520

BLAST of Csa6G483410 vs. NCBI nr
Match: gi|659080916|ref|XP_008441048.1| (PREDICTED: uncharacterized protein LOC103485275 [Cucumis melo])

HSP 1 Score: 1003.4 bits (2593), Expect = 1.4e-289
Identity = 492/520 (94.62%), Postives = 506/520 (97.31%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MV+DATTYIK+CLGGGEEEAILFCGQGTTSA+KRLQEVMGIAVPSILRERVIETLKEEER
Sbjct: 164 MVNDATTYIKKCLGGGEEEAILFCGQGTTSAVKRLQEVMGIAVPSILRERVIETLKEEER 223

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEA KK GNRPILGSFS
Sbjct: 224 WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEAYKKVGNRPILGSFS 283

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDM+SGEIDGYDAIFLSTHKFL
Sbjct: 284 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMQSGEIDGYDAIFLSTHKFL 343

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLY ENIEERE GGTPQII
Sbjct: 344 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYNENIEERETGGTPQII 403

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
           GIIRAALAFW+KEYIGYQEIEKQEH+YIERALKRLLPN+SI ILGSTS+KRQAILSFIIY
Sbjct: 404 GIIRAALAFWIKEYIGYQEIEKQEHQYIERALKRLLPNKSIGILGSTSSKRQAILSFIIY 463

Query: 301 SSTNNSLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQARG 360
           SSTNN+ PNC+TDML NPNSREKVK +YMWEETG MRAKPLHGPFVAALLSDLFGVQARG
Sbjct: 464 SSTNNTSPNCVTDMLCNPNSREKVKNIYMWEETGCMRAKPLHGPFVAALLSDLFGVQARG 523

Query: 361 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILKALE 420
           GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYM NEEFEFILKALE
Sbjct: 524 GCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMPNEEFEFILKALE 583

Query: 421 FIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFKNQCTNAEAKLAAI 480
           FIADYGQRFLPLYAFNLR GSWTLKEKEL DLLGKENYSA+HIFAFKNQCTNAEA+LAAI
Sbjct: 584 FIADYGQRFLPLYAFNLRTGSWTLKEKELADLLGKENYSANHIFAFKNQCTNAEARLAAI 643

Query: 481 VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
           VYKHKSYLESAK+IANLLPKFPPER LHEDIESSLL FRI
Sbjct: 644 VYKHKSYLESAKKIANLLPKFPPERELHEDIESSLLKFRI 683

BLAST of Csa6G483410 vs. NCBI nr
Match: gi|703088096|ref|XP_010093446.1| (putative cysteine desulfurase [Morus notabilis])

HSP 1 Score: 726.1 bits (1873), Expect = 4.4e-206
Identity = 362/530 (68.30%), Postives = 435/530 (82.08%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVH+A  YIK+CLGGG+++A+ FCG GTT+AIKRLQEVMGIAVPSILRER ++ L  EE+
Sbjct: 162 MVHEAADYIKKCLGGGQDDALFFCGSGTTAAIKRLQEVMGIAVPSILRERAMKILSNEEK 221

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGP+EHHSNLLSWRQSLAEVVEIG+DENGLLD E LR +LE+ K A +RPILGSFS
Sbjct: 222 WVVFVGPFEHHSNLLSWRQSLAEVVEIGLDENGLLDMEALRLKLESYKYA-HRPILGSFS 281

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           A SNVTGI SDTKAIA L+HQYGG  CFDFAA GPY++IDM+SG++DGYDA+FLS HKFL
Sbjct: 282 ASSNVTGINSDTKAIAQLLHQYGGFACFDFAACGPYLEIDMRSGKMDGYDAVFLSPHKFL 341

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPG+PGI+LM+K+LY+L+SS PSTCGGGTV YVNGF+EKDTLYYE+IEEREN GTPQII
Sbjct: 342 GGPGTPGIVLMSKALYRLRSSAPSTCGGGTVAYVNGFSEKDTLYYEDIEERENAGTPQII 401

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTSTKRQAILSFIIY 300
             IRAALAFWVKEY+GY+ IE+QE+ YI++A+KRLLPN+SI +LG+TS KRQAILSF+IY
Sbjct: 402 QTIRAALAFWVKEYVGYEVIERQENIYIQKAIKRLLPNKSIQVLGNTSAKRQAILSFLIY 461

Query: 301 SSTNN---SLPNCITDMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFGVQ 360
           S++N+    L NC  D     N  E+ + LYMW ETG+ R KPLHGPFVAALL+DLFG+Q
Sbjct: 462 STSNSYSRDLKNCCDDNYRTNNENEE-EGLYMWRETGNSRDKPLHGPFVAALLNDLFGIQ 521

Query: 361 ARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFILK 420
           ARGGC+CAGPYGH LL +DE  S AYR+AI KG+ G+KPGWTR+SFPYYM+N+EFEFIL 
Sbjct: 522 ARGGCACAGPYGHALLKVDEPHSHAYRSAIKKGFLGVKPGWTRISFPYYMANDEFEFILA 581

Query: 421 ALEFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIFAFK------NQCT 480
           ALEFIA YGQRFL LY F+LR G WT ++K L  L+ K+N S +++ A K       Q  
Sbjct: 582 ALEFIATYGQRFLTLYDFSLRTGGWTARKKALLCLV-KKNNSNNNVKAMKTENEVFKQSN 641

Query: 481 NAEAKLAAI-VYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
           NAE K   I V K   +LE+AK IA+LLPKFPP+R L E+I+ + L FR+
Sbjct: 642 NAETKHNGIMVNKFAMFLETAKDIAHLLPKFPPQRRLQEEIDLNFLHFRV 688

BLAST of Csa6G483410 vs. NCBI nr
Match: gi|590708692|ref|XP_007048349.1| (Chloroplastic NIFS-like cysteine desulfurase isoform 1 [Theobroma cacao])

HSP 1 Score: 724.2 bits (1868), Expect = 1.7e-205
Identity = 366/535 (68.41%), Postives = 433/535 (80.93%), Query Frame = 1

Query: 1   MVHDATTYIKRCLGGGEEEAILFCGQGTTSAIKRLQEVMGIAVPSILRERVIETLKEEER 60
           MVH+A+ Y+KRCLGGG+++AILFCG G+T+AIKRLQEVMGIAVPSILR+R++  L  E+R
Sbjct: 135 MVHEASNYVKRCLGGGQDDAILFCGSGSTAAIKRLQEVMGIAVPSILRDRLLNCLINEKR 194

Query: 61  WVVFVGPYEHHSNLLSWRQSLAEVVEIGMDENGLLDFEMLRSQLEACKKAGNRPILGSFS 120
           WVVFVGPYEHHSNLLSWRQSLAEVVEIG+DENGL++ E LR QLE  K A NRP+LGSFS
Sbjct: 195 WVVFVGPYEHHSNLLSWRQSLAEVVEIGLDENGLINIEALRRQLELYKHA-NRPLLGSFS 254

Query: 121 ACSNVTGIYSDTKAIATLIHQYGGHVCFDFAASGPYVQIDMKSGEIDGYDAIFLSTHKFL 180
           ACSNVTGI SDT+ IA L+H YGG VCFDFAASGPYV+IDM+SGE DGYDAIFLS HKFL
Sbjct: 255 ACSNVTGIQSDTREIAKLVHGYGGFVCFDFAASGPYVEIDMRSGEFDGYDAIFLSPHKFL 314

Query: 181 GGPGSPGILLMNKSLYKLKSSPPSTCGGGTVTYVNGFTEKDTLYYENIEERENGGTPQII 240
           GGPGSPGILLMNK+LY+L +SPPSTCGGGTV YVNGF EKDTLY +++EEREN GTPQII
Sbjct: 315 GGPGSPGILLMNKALYQLGASPPSTCGGGTVNYVNGFNEKDTLYVDDVEERENAGTPQII 374

Query: 241 GIIRAALAFWVKEYIGYQEIEKQEHRYIERALKRLLPNRSISILGSTS--TKRQAILSFI 300
            IIRAAL FWVKEYIGY+ IEKQE  YI++AL+RLLPN++I ILG+TS  T+RQAILSF+
Sbjct: 375 QIIRAALTFWVKEYIGYEVIEKQEKSYIQQALERLLPNQNIGILGNTSTETERQAILSFL 434

Query: 301 IYSSTNNSLPNCIT---DMLGNPNSREKVKKLYMWEETGSMRAKPLHGPFVAALLSDLFG 360
           ++S+T NS P  +    + +   + R K + L+MW ETG+ R KPLHGPFV  LL+DLFG
Sbjct: 435 VFSTT-NSCPGPVNKEGNHVDELDGRMK-RGLFMWGETGNRRDKPLHGPFVVTLLNDLFG 494

Query: 361 VQARGGCSCAGPYGHKLLNIDEACSQAYRTAIAKGYEGIKPGWTRVSFPYYMSNEEFEFI 420
           +QARGGC+CAGPYGH LL+IDE  S A+R AI KGY G KPGW RVSFPYYMSNEEFEFI
Sbjct: 495 IQARGGCACAGPYGHSLLHIDEPRSLAFREAIEKGYAGAKPGWARVSFPYYMSNEEFEFI 554

Query: 421 LKALEFIADYGQRFLPLYAFNLRNGSWTLKEKELTDLLGKENYSASHIF----AF----- 480
           L A+EFIA YGQRFL LY FNLR GSWT K+K L DL+GKE    SH+     AF     
Sbjct: 555 LAAIEFIAIYGQRFLSLYHFNLRTGSWTFKKKALKDLVGKEMSQGSHVLPLASAFPVTSV 614

Query: 481 -KNQCTNAEAKLAAIVYKHKSYLESAKRIANLLPKFPPERVLHEDIESSLLTFRI 521
            ++Q +  +    +++  + SYL+SAKRIA LLPKFP +R LHEDI+  LL +R+
Sbjct: 615 DRDQPSQYDDADVSVIRTYASYLKSAKRIAALLPKFPSQRRLHEDIDLDLLPYRL 666

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CSD_BACHD1.0e-1627.80Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM ... [more]
CSD_PYRAB1.8e-1330.37Probable cysteine desulfurase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=csd P... [more]
CSD_CHLPN2.5e-1228.40Probable cysteine desulfurase OS=Chlamydia pneumoniae GN=csd PE=3 SV=1[more]
CSD_STRCO7.4e-1225.62Probable cysteine desulfurase OS=Streptomyces coelicolor (strain ATCC BAA-471 / ... [more]
CSD_CHLMU1.3e-1130.80Probable cysteine desulfurase OS=Chlamydia muridarum (strain MoPn / Nigg) GN=csd... [more]
Match NameE-valueIdentityDescription
A0A0A0KF70_CUCSA1.1e-304100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483410 PE=4 SV=1[more]
W9R1J9_9ROSA3.0e-20668.30Putative cysteine desulfurase OS=Morus notabilis GN=L484_017507 PE=4 SV=1[more]
A0A061DJH6_THECC1.2e-20568.41Chloroplastic NIFS-like cysteine desulfurase isoform 1 OS=Theobroma cacao GN=TCM... [more]
M5X0L3_PRUPE1.1e-20368.91Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003222mg PE=4 SV=1[more]
F6I0F5_VITVI1.2e-19965.67Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0044g01150 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
gi|778717763|ref|XP_004148652.2|1.6e-304100.00PREDICTED: uncharacterized protein LOC101223213 [Cucumis sativus][more]
gi|700193156|gb|KGN48360.1|1.6e-304100.00hypothetical protein Csa_6G483410 [Cucumis sativus][more]
gi|659080916|ref|XP_008441048.1|1.4e-28994.62PREDICTED: uncharacterized protein LOC103485275 [Cucumis melo][more]
gi|703088096|ref|XP_010093446.1|4.4e-20668.30putative cysteine desulfurase [Morus notabilis][more]
gi|590708692|ref|XP_007048349.1|1.7e-20568.41Chloroplastic NIFS-like cysteine desulfurase isoform 1 [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000192Aminotrans_V_dom
IPR015421PyrdxlP-dep_Trfase_major
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015424PyrdxlP-dep_Trfase
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0031071 cysteine desulfurase activity
molecular_function GO:0008483 transaminase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G483410.1Csa6G483410.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000192Aminotransferase class V domainPFAMPF00266Aminotran_5coord: 3..299
score: 6.5
IPR015421Pyridoxal phosphate-dependent transferase, major region, subdomain 1GENE3DG3DSA:3.40.640.10coord: 1..234
score: 2.3
IPR015422Pyridoxal phosphate-dependent transferase, major region, subdomain 2GENE3DG3DSA:3.90.1150.10coord: 355..427
score: 6.1E-18coord: 235..314
score: 6.1
IPR015424Pyridoxal phosphate-dependent transferaseunknownSSF53383PLP-dependent transferasescoord: 341..420
score: 5.22E-54coord: 2..301
score: 5.22
NoneNo IPR availablePANTHERPTHR11601CYSTEINE DESULFURYLASEcoord: 338..458
score: 2.3E-238coord: 1..298
score: 2.3E
NoneNo IPR availablePANTHERPTHR11601:SF24SUBFAMILY NOT NAMEDcoord: 1..298
score: 2.3E-238coord: 338..458
score: 2.3E