MELO3C006817 (gene) Melon (DHL92) v3.5.1

BLAST of MELO3C006817 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 8.7e-178
Identity = 314/511 (61.45%), Postives = 390/511 (76.32%), Query Frame = 1

Query: 17  LHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTK-SPPKDMTDFKKMVIQFAM 76
           L  F LS ++F       +  WC KT  P+ C+YF    T  +  K  TDF K+ +Q A+
Sbjct: 11  LVLFSLSHTSFGYSPEE-VKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLAL 70

Query: 77  ERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRTEFDIQTWLS 136
           ERA +A+S T  +GSKCRN +EKAAW DC +LY+ T+L+LNQT +SS   T+ D QTWLS
Sbjct: 71  ERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLS 130

Query: 137 TALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGKVTYKKGFP 196
           TALTNLETCR    +L V +Y+ PL+ S+NVTKLISN+L++N     V   + +YK GFP
Sbjct: 131 TALTNLETCRASLEDLGVPEYVLPLL-SNNVTKLISNTLSLNK----VPYNEPSYKDGFP 190

Query: 197 SWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRE 256
           +W+  GDR+LLQ+ + + ++VVAQDGSGN  T+  A+  AA R    R+VI +K G Y E
Sbjct: 191 TWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAVA-AASRAGGSRYVIYIKAGTYNE 250

Query: 257 NLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAG 316
           N+E+  K+KNIM +GDG+  T ITG++SVGGG+TTF SATVAV G+ FIAR IT +NTAG
Sbjct: 251 NIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAG 310

Query: 317 PENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQ 376
           P NHQAVALRSG+DLSVFYRC+FEGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVLQ
Sbjct: 311 PNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQ 370

Query: 377 NCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWK 436
           NC I+ARKP N + N +TAQGRTDPNQ+TGI IHN RV A  DLKPV+S+VKT+LGRPWK
Sbjct: 371 NCNIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWK 430

Query: 437 EYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVIS 496
           +YSRTV++KT++DSL++P GW+EW GDFALNTLYY EY N GPGS  + RVKW+GYHV++
Sbjct: 431 QYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLT 490

Query: 497 NSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           + ++ S+FTV NFIAG SWLP T VPFT GL
Sbjct: 491 SPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of MELO3C006817 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 595.9 bits (1535), Expect = 4.3e-169
Identity = 295/512 (57.62%), Postives = 381/512 (74.41%), Query Frame = 1

Query: 16  FLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP-KDMTDFKKMVIQFA 75
           F+   +L +S  S      +  WC +T  P+ C+YF  H + + P K  ++F K+ ++  
Sbjct: 11  FVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLV 70

Query: 76  MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRTEFDIQTWL 135
           ++RA+ A++H   +G KCR+ +EKAAW DC+KLY  T+ ++N+T+D + K ++ D QTWL
Sbjct: 71  LDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWL 130

Query: 136 STALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGKVTYKKGF 195
           STALTNL+TCR GF EL V D + PL MS+NV+ L+ N+LAIN       N     K GF
Sbjct: 131 STALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKVPF---NYTPPEKDGF 190

Query: 196 PSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 255
           PSW+  GDR+LLQS++ K + VVA+DGSGN+ T+  A++ A+    SGRFVI VK+GVY 
Sbjct: 191 PSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASG---SGRFVIYVKQGVYS 250

Query: 256 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTA 315
           ENLEI  + KN+ML GDG+  T ITG++SVGGG+TTFNSATVA  G+GFIARGITF+NTA
Sbjct: 251 ENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTA 310

Query: 316 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 375
           G  N QAVALRSG+DLSVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VL
Sbjct: 311 GASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVL 370

Query: 376 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 435
           QNC I+AR+P   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + KTYLGRPW
Sbjct: 371 QNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPW 430

Query: 436 KEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 495
           ++YSRTVFMKT +DSL+DP GWLEWDG+FAL TL+Y E+ N GPG+  S RV W G+ V+
Sbjct: 431 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 490

Query: 496 SNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            +++EAS+FTV  F+AG SW+P++ VPFT GL
Sbjct: 491 GSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of MELO3C006817 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 5.5e-164
Identity = 291/528 (55.11%), Postives = 372/528 (70.45%), Query Frame = 1

Query: 15  LFLHFFLL---SSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDFKKMV 74
           LFLH  LL        +  A      WC KT YP+ CK YF NH     P  +++F+ M+
Sbjct: 11  LFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVML 70

Query: 75  IQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKR----- 134
           ++ AM+RA+SA +     G  C + K++A  ADC+ LY +TI+QLN+TL   + +     
Sbjct: 71  VEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAK 130

Query: 135 --TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAV 194
             T+FD QTWLSTALTN ETCR G ++LNV D+I P++ +  ++ LISN LA+N A    
Sbjct: 131 SCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTA 190

Query: 195 GN-GKVTY-KKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRK-T 254
           GN G  T  +KGFP+WLS  D+RLL++   + +LVVA+DGSG++ TV AA++ A +RK T
Sbjct: 191 GNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAIDVAGRRKVT 250

Query: 255 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 314
           SGRFVI VKRG+Y+EN+ +     +IML+GDGMR T ITG RSV GG TT+NSAT  + G
Sbjct: 251 SGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEG 310

Query: 315 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 374
             FIA+GITF+NTAGP   QAVALRS +DLS+FY+C+ EGYQDTL VHSQRQFY+ECYIY
Sbjct: 311 LHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIY 370

Query: 375 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 434
           GTVDFIFGNAA V QNC+I  R+P+ GQ N +TAQGR DP QNTGISIHNSR++   DLK
Sbjct: 371 GTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLK 430

Query: 435 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW-DGD-FALNTLYYGEYNNIGP 494
           PV  TVKTY+GRPW ++SRTV ++TY+D++V P GW  W +G  F L+TL+Y EY N GP
Sbjct: 431 PVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGP 490

Query: 495 GSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            S    RV WKG+HV+  +++AS FTV  FIAG +WLP T +PFT GL
Sbjct: 491 ASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of MELO3C006817 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.3e-161
Identity = 288/530 (54.34%), Postives = 365/530 (68.87%), Query Frame = 1

Query: 14  FLFLHFFLL------SSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDF 73
           FL LH  L+        +  +   S  ID WC KT YP  CK YF  H     P  +++F
Sbjct: 11  FLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70

Query: 74  KKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRT 133
           + ++++ AM+RA+SA          C + K++A  ADC+ LY +T++QLN+TL   + +T
Sbjct: 71  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130

Query: 134 -----EFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASA 193
                +FD QTWLSTALTN ETCR G ++LNV D+  P++ +  ++ LISN LA+N A  
Sbjct: 131 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALL 190

Query: 194 AVGNGKVTY--KKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRK 253
             G    T    KGFP+W+S  +RRLLQ  S + +LVVA+DGSG++ TV AA++ A +RK
Sbjct: 191 TAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRK 250

Query: 254 -TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAV 313
            TSGRFVI VKRG+Y+ENL +     NIML+GDGMR+T ITG RSV GG TT++SAT  +
Sbjct: 251 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

Query: 314 TGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 373
            G  FIA+GI FQNTAGP   QAVALRS +DLS+FYRC+ EGYQDTL VHSQRQFY+ECY
Sbjct: 311 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 370

Query: 374 IYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 433
           IYGTVDFIFGNAAVV QNC+I  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   D
Sbjct: 371 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 430

Query: 434 LKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNI 493
           LKPV  +VKTY+GRPW  YSRTV +KTYIDS+V P GW  W     + L+TL+Y EY NI
Sbjct: 431 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 490

Query: 494 GPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           GP S    RV+WKG+HV+S +++AS F+V  FIAG +WLP + +PFT  L
Sbjct: 491 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of MELO3C006817 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 9.4e-156
Identity = 276/525 (52.57%), Postives = 360/525 (68.57%), Query Frame = 1

Query: 26  AFSKPASNG-----IDWWCKKTTYPETCKYFFNHGTKS-----PPKDMTDFKKMVIQFAM 85
           A   P +NG     + WWC KT + ETC Y+F   +++     PP+  ++F +M+++ A+
Sbjct: 70  AAPSPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVAL 129

Query: 86  ERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL-------DSSTKRTEF 145
           ++A+   S T   G  C N + KAAW+DC+ L+QNT+ QLN+TL        S  K T+F
Sbjct: 130 DQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDF 189

Query: 146 DIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGKV 205
           D QTWLSTA TN+ETCR+G  +LNV D++ P+I + N++ LI N LA+N       +   
Sbjct: 190 DAQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTT 249

Query: 206 T--YKKGFPSWLSGGDRRLLQSAS---TKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGR 265
           T  +K+ FPSW+S  +RRLL SAS   +   LVVAQD SG++ ++ AA+  AA+R+   R
Sbjct: 250 TANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSR 309

Query: 266 FVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF 325
           FVI VK+GVYREN+++G+   NIML+GDG R T IT  RSV  G TT+NSAT    G+ F
Sbjct: 310 FVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRF 369

Query: 326 IARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTV 385
           +A+ +TF NTAGP   QAVA+RS +DLSVFYR    G+QDTLY+HSQRQF++ECYI GT+
Sbjct: 370 VAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTI 429

Query: 386 DFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVE 445
           DFIFGNAAVV QNCMI  R+P++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV 
Sbjct: 430 DFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVI 489

Query: 446 STVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNIGPGSP 505
              KTYLGRPW+ YSR   MKTYID+ + P GW  W    +FALNT++YGEY N GPGS 
Sbjct: 490 RAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSS 549

Query: 506 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
              RV+WKG+H I+++  AS FTV + IAG SWLP T VPF  GL
Sbjct: 550 TRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of MELO3C006817 vs. TrEMBL
Match: A0A0A0L9G4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G175720 PE=4 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 2.0e-298
Identity = 510/527 (96.77%), Postives = 516/527 (97.91%), Query Frame = 1

Query: 1   MAGLSMETSLVGVFLFLHFFLL-SSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSP 60
           MAGLSM TS  GVF+FLHFFLL SSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSP
Sbjct: 46  MAGLSMGTSF-GVFIFLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSP 105

Query: 61  PKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL 120
           PKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL
Sbjct: 106 PKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL 165

Query: 121 DSSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNA 180
           DSSTK TEFDIQTWLSTALTNLETCRTGFAELNV DYI PLIMSDNVT+LISNSLAINNA
Sbjct: 166 DSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNA 225

Query: 181 SAAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRK 240
           SA VGNGK TYKKGFPSWLSGGDRRLLQS+STKVDLVVAQDGSGNYTTVGAALEEAAKRK
Sbjct: 226 SAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRK 285

Query: 241 TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVT 300
           TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVT
Sbjct: 286 TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVT 345

Query: 301 GEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYI 360
           GEGFIARGITF+NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYI
Sbjct: 346 GEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYI 405

Query: 361 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
           YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 406 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 465

Query: 421 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPG 480
           KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDP GWLEWDGDFALNTLYYGEYNNIGPG
Sbjct: 466 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPG 525

Query: 481 SPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           SPISQRVKWKGYHVI+N TEASEFTVQNFIAGQSWLP+TEVPFTPGL
Sbjct: 526 SPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

BLAST of MELO3C006817 vs. TrEMBL
Match: A0A061G787_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_014942 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 3.1e-222
Identity = 383/522 (73.37%), Postives = 441/522 (84.48%), Query Frame = 1

Query: 6   METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKS-PPKDMT 65
           M T L    +F+ F L+  +  S   +NGID+WC +T  P+ CKYF     KS  PK  +
Sbjct: 1   MATKLALFVIFMSFSLVPPT-LSSVVTNGIDYWCDRTPNPQPCKYFMRQKGKSYVPKQKS 60

Query: 66  DFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTK 125
           +F+KM +Q A++RAL+A+SH KG+G KCRN KEKAAWADCLKLY+ TILQLNQTLDS TK
Sbjct: 61  EFRKMAVQIALDRALNAQSHNKGLGKKCRNDKEKAAWADCLKLYEETILQLNQTLDS-TK 120

Query: 126 RTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVG 185
            T+FD+QTWLSTALTNLETCR GF EL V DYI PL MS+NV+KLISN+LA+NN ++ V 
Sbjct: 121 CTDFDVQTWLSTALTNLETCRAGFVELGVSDYILPL-MSNNVSKLISNTLALNNNASFVP 180

Query: 186 NGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRF 245
               TYK GFP WL  GDR+LLQS+S   +LVVAQDGSGN+ T+  AL+ AAKR  SGRF
Sbjct: 181 Q---TYKHGFPRWLKAGDRKLLQSSSPTPNLVVAQDGSGNHRTIKEALDAAAKRSGSGRF 240

Query: 246 VIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFI 305
           VI VKRGVY+ENLEIG+K+KNIML+GDG+R+T ITG+RSVGGGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKRGVYKENLEIGNKLKNIMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 306 ARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVD 365
           ARGITF+NTAGP+NHQAVALRSG+DLSVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVD 360

Query: 366 FIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVES 425
           FIFGNAAVVLQNCMIYAR+PM+ QKN VTAQGRTDPNQNTGISIHNSRVMA +DL PV S
Sbjct: 361 FIFGNAAVVLQNCMIYARRPMDKQKNIVTAQGRTDPNQNTGISIHNSRVMAANDLTPVLS 420

Query: 426 TVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQ 485
           + KT+LGRPWKEYSRTVFM+TY+DSLVDP GW EWDGDFALNTLYYGEY NIGP +P S+
Sbjct: 421 SFKTFLGRPWKEYSRTVFMQTYLDSLVDPAGWFEWDGDFALNTLYYGEYKNIGPAAPTSR 480

Query: 486 RVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           RV WKGY VI+++TEAS+FTV NFIAG+SWLP+T +PFT GL
Sbjct: 481 RVTWKGYRVITSATEASKFTVTNFIAGRSWLPDTGIPFTSGL 516

BLAST of MELO3C006817 vs. TrEMBL
Match: A0A068VJK2_COFCA (Pectinesterase OS=Coffea canephora GN=GSCOC_T00009515001 PE=4 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 1.1e-219
Identity = 380/519 (73.22%), Postives = 438/519 (84.39%), Query Frame = 1

Query: 14  FLFLHFFLLSS---SAFSK-PASNGIDWWCKKTTYPETCKYFF-NHGTKS-PPKDMTDFK 73
           +LF  F  LSS     FSK P+S  I+WWC +T YPE C YFF NHGT    PK+  D K
Sbjct: 7   WLFFTFISLSSFFSPCFSKNPSSGDINWWCNQTPYPEQCNYFFLNHGTNHFMPKEKADVK 66

Query: 74  KMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRTE 133
           KM +Q  +ERAL A++HT+ + SKCRN +EKAAWADCLKLY+NTILQLN+TLDS+TK T+
Sbjct: 67  KMAMQITLERALKAKTHTENLESKCRNEREKAAWADCLKLYENTILQLNRTLDSNTKCTD 126

Query: 134 FDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGK 193
           FD QTWLSTALTNLETCR+GF EL V D++ PL MS+NV+KLI N+LA+NN +  V    
Sbjct: 127 FDAQTWLSTALTNLETCRSGFQELGVSDFVLPL-MSNNVSKLICNTLALNN-NINVSVET 186

Query: 194 VTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQ 253
            TYK GFP+W++ GDR+LLQS+  + +L+VAQDGSGNY T+ AAL+ AAKR  SGRFVIQ
Sbjct: 187 QTYKDGFPTWVTPGDRKLLQSSLVRPNLIVAQDGSGNYRTIKAALDAAAKRSGSGRFVIQ 246

Query: 254 VKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARG 313
           VKRG YRENLEIG+KMKNIML+G GMR T ITG+RSVGGGSTTF+SATVAVTG+GFIA+G
Sbjct: 247 VKRGTYRENLEIGNKMKNIMLLGAGMRDTIITGSRSVGGGSTTFDSATVAVTGDGFIAQG 306

Query: 314 ITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 373
           ITF+NTAGP+NHQAVALRSG+DLSVF+RC FEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 307 ITFRNTAGPQNHQAVALRSGSDLSVFHRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 366

Query: 374 GNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVK 433
           GNAAVVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DL+PV S+ K
Sbjct: 367 GNAAVVLQNCMIYARRPMDKQKNTITAQGRTDPNQNTGISIHNSRVMAASDLRPVLSSFK 426

Query: 434 TYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVK 493
           TYLGRPWK+YSRTVF++TY+DSLVDP GWLEWDG+FA NTLYYGEY N GPGS  S+RVK
Sbjct: 427 TYLGRPWKQYSRTVFIQTYLDSLVDPAGWLEWDGNFAFNTLYYGEYKNSGPGSSTSRRVK 486

Query: 494 WKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           W+GYHVI+N TEAS FTV NFIAG+SWLP T VPF  GL
Sbjct: 487 WRGYHVITNPTEASRFTVANFIAGRSWLPATNVPFAAGL 523

BLAST of MELO3C006817 vs. TrEMBL
Match: W9SEQ5_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_000799 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 1.6e-218
Identity = 381/525 (72.57%), Postives = 435/525 (82.86%), Query Frame = 1

Query: 8   TSLVGVF-LFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFF----NHGTKSPPKD 67
           T  +G+F LFL F  L S   S   SNGIDWWC +T YPETCK  +    NH     PK 
Sbjct: 2   TKNLGLFVLFLSFSSLLSLTLSSVHSNGIDWWCNRTPYPETCKQSWRDNDNHQKLFIPKL 61

Query: 68  MTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSS 127
             DFKK+ +Q ++++AL A+SH K +GSKCRN KEKAAW+DCLKLY++ IL LN+TLD S
Sbjct: 62  KADFKKLELQISLKQALKADSHNKWLGSKCRNDKEKAAWSDCLKLYEDAILLLNKTLDPS 121

Query: 128 TKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAA 187
           TK T+FD QTWLSTALTNL+TCR GF EL V D++WPL MS+NV+KLISN+LA NNASA 
Sbjct: 122 TKCTDFDAQTWLSTALTNLDTCRAGFVELGVSDFVWPL-MSNNVSKLISNTLATNNASAP 181

Query: 188 VGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSG 247
                  YK GFP WLS GDR+LLQS+S   +LVVA+DGSG++ T+ AAL+ AAKR  SG
Sbjct: 182 PVTN--AYKDGFPIWLSPGDRKLLQSSSQSPNLVVAKDGSGDHETIKAALDAAAKRSGSG 241

Query: 248 RFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGS-TTFNSATVAVTGE 307
           RF+I VKRGVY ENLEIG+K+KNIML+GDG+R+T ITG+RSVGGG  TTFNSATVAVTGE
Sbjct: 242 RFIIHVKRGVYNENLEIGNKLKNIMLVGDGLRYTIITGSRSVGGGGYTTFNSATVAVTGE 301

Query: 308 GFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 367
           GFIA+GITF+NTAGPENHQAVALRSGADLSVFYRC FEGYQDTLYVHSQRQFYKECYIYG
Sbjct: 302 GFIAQGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYG 361

Query: 368 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKP 427
           TVDFIFGNAA VLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DLKP
Sbjct: 362 TVDFIFGNAAAVLQNCMIYARRPMSKQKNTITAQGRTDPNQNTGISIHNSRVMAAADLKP 421

Query: 428 VESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSP 487
           V S+ KTYLGRPWK+YSRTVFM+TY+DSLVDP GWLEWDGDFALNTLYYGEY N+GP + 
Sbjct: 422 VLSSFKTYLGRPWKQYSRTVFMQTYLDSLVDPAGWLEWDGDFALNTLYYGEYRNLGPAAS 481

Query: 488 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            S+RVKW GY VI++S+EAS+FTV NFIAGQSWLP T V FT GL
Sbjct: 482 TSRRVKWSGYRVITSSSEASQFTVANFIAGQSWLPATGVRFTAGL 523

BLAST of MELO3C006817 vs. TrEMBL
Match: F6HAP0_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_16s0022g00700 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-216
Identity = 377/515 (73.20%), Postives = 431/515 (83.69%), Query Frame = 1

Query: 20  FLLSSSAFSKPA----SNGIDWWCKKTTYPETCKYFFNHGTK--SPPKDMTDFKKMVIQF 79
           F+LS S FS       S  ++WWC KT YP  CKYF +HG    + PK  ++F+KM +Q 
Sbjct: 11  FMLSLSFFSSTILASDSGDVNWWCDKTPYPAPCKYFMSHGGHKYNAPKKKSEFQKMAMQV 70

Query: 80  AMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRTEFDIQTW 139
           AMERAL+A+SH K +GSKCRN +EKAAWADCLK YQ+TI QLNQTLD +TK T+FD QTW
Sbjct: 71  AMERALTAQSHNKWLGSKCRNEREKAAWADCLKQYQDTIQQLNQTLDPATKCTDFDQQTW 130

Query: 140 LSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGKVTYKKG 199
           LSTALTNL+TCR GF EL V D++ PL MS+NV+KLISNSLA+ N        K TYK+G
Sbjct: 131 LSTALTNLDTCRAGFVELGVSDFVLPL-MSNNVSKLISNSLAMKNDIPE----KHTYKEG 190

Query: 200 FPSWLSGGDRRLLQSAST--KVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRG 259
           FPSW+  GDRRLLQS ST  K +LVVAQDGSGNY T+ AA+E AAKR  SGR+VI VK+G
Sbjct: 191 FPSWVKAGDRRLLQSTSTAAKANLVVAQDGSGNYKTIKAAIEAAAKRSGSGRYVIHVKKG 250

Query: 260 VYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQ 319
           VY+EN+EIG+KMKNIML+GDG+R T ITG+RSVGGG TTFNSATVAVTGEGFIARGITF+
Sbjct: 251 VYKENIEIGNKMKNIMLVGDGLRNTIITGSRSVGGGFTTFNSATVAVTGEGFIARGITFR 310

Query: 320 NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 379
           NTAGP+NHQAVALRSG+DLSVFY+C+FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA
Sbjct: 311 NTAGPQNHQAVALRSGSDLSVFYQCSFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 370

Query: 380 VVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLG 439
           VVLQNCMIYAR+PM+ QKN VTAQGRTDPNQNTGISIHNSRVMA  DLKPV S+ KTYLG
Sbjct: 371 VVLQNCMIYARRPMDKQKNVVTAQGRTDPNQNTGISIHNSRVMAATDLKPVLSSFKTYLG 430

Query: 440 RPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGY 499
           RPWKEYSRTV++ TY+D+LVD  GWLEWDG+FALNTLYYGEY N GPGS  S RVKW+GY
Sbjct: 431 RPWKEYSRTVYLGTYLDTLVDSAGWLEWDGNFALNTLYYGEYKNFGPGSSTSGRVKWRGY 490

Query: 500 HVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            VI+++TEAS+F+V NFIAGQSWLP T VPF  GL
Sbjct: 491 RVITSATEASKFSVANFIAGQSWLPATGVPFRSGL 520

BLAST of MELO3C006817 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 595.9 bits (1535), Expect = 2.4e-170
Identity = 295/512 (57.62%), Postives = 381/512 (74.41%), Query Frame = 1

Query: 16  FLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP-KDMTDFKKMVIQFA 75
           F+   +L +S  S      +  WC +T  P+ C+YF  H + + P K  ++F K+ ++  
Sbjct: 11  FVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLV 70

Query: 76  MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRTEFDIQTWL 135
           ++RA+ A++H   +G KCR+ +EKAAW DC+KLY  T+ ++N+T+D + K ++ D QTWL
Sbjct: 71  LDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWL 130

Query: 136 STALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGKVTYKKGF 195
           STALTNL+TCR GF EL V D + PL MS+NV+ L+ N+LAIN       N     K GF
Sbjct: 131 STALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKVPF---NYTPPEKDGF 190

Query: 196 PSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 255
           PSW+  GDR+LLQS++ K + VVA+DGSGN+ T+  A++ A+    SGRFVI VK+GVY 
Sbjct: 191 PSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASG---SGRFVIYVKQGVYS 250

Query: 256 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTA 315
           ENLEI  + KN+ML GDG+  T ITG++SVGGG+TTFNSATVA  G+GFIARGITF+NTA
Sbjct: 251 ENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTA 310

Query: 316 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 375
           G  N QAVALRSG+DLSVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VL
Sbjct: 311 GASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVL 370

Query: 376 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 435
           QNC I+AR+P   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + KTYLGRPW
Sbjct: 371 QNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPW 430

Query: 436 KEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 495
           ++YSRTVFMKT +DSL+DP GWLEWDG+FAL TL+Y E+ N GPG+  S RV W G+ V+
Sbjct: 431 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 490

Query: 496 SNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            +++EAS+FTV  F+AG SW+P++ VPFT GL
Sbjct: 491 GSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of MELO3C006817 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 578.9 bits (1491), Expect = 3.1e-165
Identity = 291/528 (55.11%), Postives = 372/528 (70.45%), Query Frame = 1

Query: 15  LFLHFFLL---SSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDFKKMV 74
           LFLH  LL        +  A      WC KT YP+ CK YF NH     P  +++F+ M+
Sbjct: 11  LFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVML 70

Query: 75  IQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKR----- 134
           ++ AM+RA+SA +     G  C + K++A  ADC+ LY +TI+QLN+TL   + +     
Sbjct: 71  VEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAK 130

Query: 135 --TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAV 194
             T+FD QTWLSTALTN ETCR G ++LNV D+I P++ +  ++ LISN LA+N A    
Sbjct: 131 SCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTA 190

Query: 195 GN-GKVTY-KKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRK-T 254
           GN G  T  +KGFP+WLS  D+RLL++   + +LVVA+DGSG++ TV AA++ A +RK T
Sbjct: 191 GNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAAIDVAGRRKVT 250

Query: 255 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 314
           SGRFVI VKRG+Y+EN+ +     +IML+GDGMR T ITG RSV GG TT+NSAT  + G
Sbjct: 251 SGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEG 310

Query: 315 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 374
             FIA+GITF+NTAGP   QAVALRS +DLS+FY+C+ EGYQDTL VHSQRQFY+ECYIY
Sbjct: 311 LHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIY 370

Query: 375 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 434
           GTVDFIFGNAA V QNC+I  R+P+ GQ N +TAQGR DP QNTGISIHNSR++   DLK
Sbjct: 371 GTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLK 430

Query: 435 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW-DGD-FALNTLYYGEYNNIGP 494
           PV  TVKTY+GRPW ++SRTV ++TY+D++V P GW  W +G  F L+TL+Y EY N GP
Sbjct: 431 PVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGP 490

Query: 495 GSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            S    RV WKG+HV+  +++AS FTV  FIAG +WLP T +PFT GL
Sbjct: 491 ASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of MELO3C006817 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 569.7 bits (1467), Expect = 1.9e-162
Identity = 288/530 (54.34%), Postives = 365/530 (68.87%), Query Frame = 1

Query: 14  FLFLHFFLL------SSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDF 73
           FL LH  L+        +  +   S  ID WC KT YP  CK YF  H     P  +++F
Sbjct: 11  FLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70

Query: 74  KKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRT 133
           + ++++ AM+RA+SA          C + K++A  ADC+ LY +T++QLN+TL   + +T
Sbjct: 71  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130

Query: 134 -----EFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASA 193
                +FD QTWLSTALTN ETCR G ++LNV D+  P++ +  ++ LISN LA+N A  
Sbjct: 131 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALL 190

Query: 194 AVGNGKVTY--KKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRK 253
             G    T    KGFP+W+S  +RRLLQ  S + +LVVA+DGSG++ TV AA++ A +RK
Sbjct: 191 TAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRK 250

Query: 254 -TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAV 313
            TSGRFVI VKRG+Y+ENL +     NIML+GDGMR+T ITG RSV GG TT++SAT  +
Sbjct: 251 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

Query: 314 TGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 373
            G  FIA+GI FQNTAGP   QAVALRS +DLS+FYRC+ EGYQDTL VHSQRQFY+ECY
Sbjct: 311 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 370

Query: 374 IYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 433
           IYGTVDFIFGNAAVV QNC+I  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   D
Sbjct: 371 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 430

Query: 434 LKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNI 493
           LKPV  +VKTY+GRPW  YSRTV +KTYIDS+V P GW  W     + L+TL+Y EY NI
Sbjct: 431 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 490

Query: 494 GPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           GP S    RV+WKG+HV+S +++AS F+V  FIAG +WLP + +PFT  L
Sbjct: 491 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of MELO3C006817 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 551.6 bits (1420), Expect = 5.3e-157
Identity = 276/525 (52.57%), Postives = 360/525 (68.57%), Query Frame = 1

Query: 26  AFSKPASNG-----IDWWCKKTTYPETCKYFFNHGTKS-----PPKDMTDFKKMVIQFAM 85
           A   P +NG     + WWC KT + ETC Y+F   +++     PP+  ++F +M+++ A+
Sbjct: 70  AAPSPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVAL 129

Query: 86  ERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL-------DSSTKRTEF 145
           ++A+   S T   G  C N + KAAW+DC+ L+QNT+ QLN+TL        S  K T+F
Sbjct: 130 DQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDF 189

Query: 146 DIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGKV 205
           D QTWLSTA TN+ETCR+G  +LNV D++ P+I + N++ LI N LA+N       +   
Sbjct: 190 DAQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTT 249

Query: 206 T--YKKGFPSWLSGGDRRLLQSAS---TKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGR 265
           T  +K+ FPSW+S  +RRLL SAS   +   LVVAQD SG++ ++ AA+  AA+R+   R
Sbjct: 250 TANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSR 309

Query: 266 FVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF 325
           FVI VK+GVYREN+++G+   NIML+GDG R T IT  RSV  G TT+NSAT    G+ F
Sbjct: 310 FVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRF 369

Query: 326 IARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTV 385
           +A+ +TF NTAGP   QAVA+RS +DLSVFYR    G+QDTLY+HSQRQF++ECYI GT+
Sbjct: 370 VAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTI 429

Query: 386 DFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVE 445
           DFIFGNAAVV QNCMI  R+P++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV 
Sbjct: 430 DFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVI 489

Query: 446 STVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNIGPGSP 505
              KTYLGRPW+ YSR   MKTYID+ + P GW  W    +FALNT++YGEY N GPGS 
Sbjct: 490 RAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSS 549

Query: 506 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
              RV+WKG+H I+++  AS FTV + IAG SWLP T VPF  GL
Sbjct: 550 TRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of MELO3C006817 vs. TAIR10
Match: AT1G23200.1 (AT1G23200.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 458.4 bits (1178), Expect = 6.1e-129
Identity = 252/528 (47.73%), Postives = 327/528 (61.93%), Query Frame = 1

Query: 39  CKKTTYPETCKYFFNHGTKSPPKDMTD---FKKMVIQFAMERALSAESHTKGVGSKCRNG 98
           CK+T YP  C +  ++       D TD   F  +V+   M++A+  + H      K  + 
Sbjct: 38  CKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAV--QLHRLVSSLKQHHS 97

Query: 99  KEKAAWA---DCLKLYQNTILQLNQTLDSSTKRTE-FDIQTWLSTALTNLETCRTGFAEL 158
             K A +   DCL+LY++TI QLN +  S  + +   D QT LS A+ N +TCR GF + 
Sbjct: 98  LHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDF 157

Query: 159 NVLDYI---WPLIMSDNVTKLISNSLAINNASA----------AVGNGKVTYKKG----- 218
            +       +P+    N+TK ISNSLA+  A+A          + G  K + ++      
Sbjct: 158 KLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGG 217

Query: 219 ------------FPSWLSGGDRRLLQSAST--KVDLVVAQDGSGNYTTVGAALEEAAKR- 278
                       FPSW    DR+LL+ + T  K DLVVA+DGSG+YT++  A+  AAK  
Sbjct: 218 SHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLP 277

Query: 279 KTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAV 338
           + + R VI VK GVYREN+ I   +KN+M+IGDG+  T +TGNR+V  G+TTF SAT AV
Sbjct: 278 RRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAV 337

Query: 339 TGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 398
           +G GFIA+GITF+NTAGPE HQAVALRS +D SVFY C+F+GYQDTLY+HS RQF + C 
Sbjct: 338 SGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCN 397

Query: 399 IYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 458
           IYGTVDFIFG+A  +LQNC IYARKPM+GQKN +TAQ R +P++ TG  I +S V    +
Sbjct: 398 IYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE 457

Query: 459 LKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGP 518
                    TYLGRPW+ +SRTVFMK  + +LV P GWL W G FAL+TLYYGEY N G 
Sbjct: 458 ---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGA 517

Query: 519 GSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           G+ +S RVKW GYHVI   TEA +FTV+NF+ G  W+  T VP   GL
Sbjct: 518 GASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

BLAST of MELO3C006817 vs. NCBI nr
Match: gi|659077230|ref|XP_008439095.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1073.2 bits (2774), Expect = 1.6e-310
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 1

Query: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60
           MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP
Sbjct: 46  MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105

Query: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120
           KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD
Sbjct: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165

Query: 121 SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180
           SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS
Sbjct: 166 SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 225

Query: 181 AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240
           AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT
Sbjct: 226 AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285

Query: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300
           SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345

Query: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 360
           EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 346 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 405

Query: 361 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 420
           GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK
Sbjct: 406 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 465

Query: 421 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 480
           PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS
Sbjct: 466 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 525

Query: 481 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL
Sbjct: 526 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 571

BLAST of MELO3C006817 vs. NCBI nr
Match: gi|778679320|ref|XP_004148139.2| (PREDICTED: pectinesterase 2 [Cucumis sativus])

HSP 1 Score: 1032.3 bits (2668), Expect = 2.9e-298
Identity = 510/527 (96.77%), Postives = 516/527 (97.91%), Query Frame = 1

Query: 1   MAGLSMETSLVGVFLFLHFFLL-SSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSP 60
           MAGLSM TS  GVF+FLHFFLL SSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSP
Sbjct: 46  MAGLSMGTSF-GVFIFLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSP 105

Query: 61  PKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL 120
           PKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL
Sbjct: 106 PKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL 165

Query: 121 DSSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNA 180
           DSSTK TEFDIQTWLSTALTNLETCRTGFAELNV DYI PLIMSDNVT+LISNSLAINNA
Sbjct: 166 DSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNA 225

Query: 181 SAAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRK 240
           SA VGNGK TYKKGFPSWLSGGDRRLLQS+STKVDLVVAQDGSGNYTTVGAALEEAAKRK
Sbjct: 226 SAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRK 285

Query: 241 TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVT 300
           TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVT
Sbjct: 286 TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVT 345

Query: 301 GEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYI 360
           GEGFIARGITF+NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYI
Sbjct: 346 GEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYI 405

Query: 361 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 420
           YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL
Sbjct: 406 YGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDL 465

Query: 421 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPG 480
           KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDP GWLEWDGDFALNTLYYGEYNNIGPG
Sbjct: 466 KPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPG 525

Query: 481 SPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           SPISQRVKWKGYHVI+N TEASEFTVQNFIAGQSWLP+TEVPFTPGL
Sbjct: 526 SPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

BLAST of MELO3C006817 vs. NCBI nr
Match: gi|590671731|ref|XP_007038413.1| (Pectinesterase [Theobroma cacao])

HSP 1 Score: 779.2 bits (2011), Expect = 4.4e-222
Identity = 383/522 (73.37%), Postives = 441/522 (84.48%), Query Frame = 1

Query: 6   METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKS-PPKDMT 65
           M T L    +F+ F L+  +  S   +NGID+WC +T  P+ CKYF     KS  PK  +
Sbjct: 1   MATKLALFVIFMSFSLVPPT-LSSVVTNGIDYWCDRTPNPQPCKYFMRQKGKSYVPKQKS 60

Query: 66  DFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTK 125
           +F+KM +Q A++RAL+A+SH KG+G KCRN KEKAAWADCLKLY+ TILQLNQTLDS TK
Sbjct: 61  EFRKMAVQIALDRALNAQSHNKGLGKKCRNDKEKAAWADCLKLYEETILQLNQTLDS-TK 120

Query: 126 RTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVG 185
            T+FD+QTWLSTALTNLETCR GF EL V DYI PL MS+NV+KLISN+LA+NN ++ V 
Sbjct: 121 CTDFDVQTWLSTALTNLETCRAGFVELGVSDYILPL-MSNNVSKLISNTLALNNNASFVP 180

Query: 186 NGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRF 245
               TYK GFP WL  GDR+LLQS+S   +LVVAQDGSGN+ T+  AL+ AAKR  SGRF
Sbjct: 181 Q---TYKHGFPRWLKAGDRKLLQSSSPTPNLVVAQDGSGNHRTIKEALDAAAKRSGSGRF 240

Query: 246 VIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFI 305
           VI VKRGVY+ENLEIG+K+KNIML+GDG+R+T ITG+RSVGGGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKRGVYKENLEIGNKLKNIMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 306 ARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVD 365
           ARGITF+NTAGP+NHQAVALRSG+DLSVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVD 360

Query: 366 FIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVES 425
           FIFGNAAVVLQNCMIYAR+PM+ QKN VTAQGRTDPNQNTGISIHNSRVMA +DL PV S
Sbjct: 361 FIFGNAAVVLQNCMIYARRPMDKQKNIVTAQGRTDPNQNTGISIHNSRVMAANDLTPVLS 420

Query: 426 TVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQ 485
           + KT+LGRPWKEYSRTVFM+TY+DSLVDP GW EWDGDFALNTLYYGEY NIGP +P S+
Sbjct: 421 SFKTFLGRPWKEYSRTVFMQTYLDSLVDPAGWFEWDGDFALNTLYYGEYKNIGPAAPTSR 480

Query: 486 RVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           RV WKGY VI+++TEAS+FTV NFIAG+SWLP+T +PFT GL
Sbjct: 481 RVTWKGYRVITSATEASKFTVTNFIAGRSWLPDTGIPFTSGL 516

BLAST of MELO3C006817 vs. NCBI nr
Match: gi|661873590|emb|CDP20891.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 770.8 bits (1989), Expect = 1.6e-219
Identity = 380/519 (73.22%), Postives = 438/519 (84.39%), Query Frame = 1

Query: 14  FLFLHFFLLSS---SAFSK-PASNGIDWWCKKTTYPETCKYFF-NHGTKS-PPKDMTDFK 73
           +LF  F  LSS     FSK P+S  I+WWC +T YPE C YFF NHGT    PK+  D K
Sbjct: 7   WLFFTFISLSSFFSPCFSKNPSSGDINWWCNQTPYPEQCNYFFLNHGTNHFMPKEKADVK 66

Query: 74  KMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKRTE 133
           KM +Q  +ERAL A++HT+ + SKCRN +EKAAWADCLKLY+NTILQLN+TLDS+TK T+
Sbjct: 67  KMAMQITLERALKAKTHTENLESKCRNEREKAAWADCLKLYENTILQLNRTLDSNTKCTD 126

Query: 134 FDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAAVGNGK 193
           FD QTWLSTALTNLETCR+GF EL V D++ PL MS+NV+KLI N+LA+NN +  V    
Sbjct: 127 FDAQTWLSTALTNLETCRSGFQELGVSDFVLPL-MSNNVSKLICNTLALNN-NINVSVET 186

Query: 194 VTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQ 253
            TYK GFP+W++ GDR+LLQS+  + +L+VAQDGSGNY T+ AAL+ AAKR  SGRFVIQ
Sbjct: 187 QTYKDGFPTWVTPGDRKLLQSSLVRPNLIVAQDGSGNYRTIKAALDAAAKRSGSGRFVIQ 246

Query: 254 VKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARG 313
           VKRG YRENLEIG+KMKNIML+G GMR T ITG+RSVGGGSTTF+SATVAVTG+GFIA+G
Sbjct: 247 VKRGTYRENLEIGNKMKNIMLLGAGMRDTIITGSRSVGGGSTTFDSATVAVTGDGFIAQG 306

Query: 314 ITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 373
           ITF+NTAGP+NHQAVALRSG+DLSVF+RC FEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 307 ITFRNTAGPQNHQAVALRSGSDLSVFHRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 366

Query: 374 GNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVK 433
           GNAAVVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DL+PV S+ K
Sbjct: 367 GNAAVVLQNCMIYARRPMDKQKNTITAQGRTDPNQNTGISIHNSRVMAASDLRPVLSSFK 426

Query: 434 TYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVK 493
           TYLGRPWK+YSRTVF++TY+DSLVDP GWLEWDG+FA NTLYYGEY N GPGS  S+RVK
Sbjct: 427 TYLGRPWKQYSRTVFIQTYLDSLVDPAGWLEWDGNFAFNTLYYGEYKNSGPGSSTSRRVK 486

Query: 494 WKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
           W+GYHVI+N TEAS FTV NFIAG+SWLP T VPF  GL
Sbjct: 487 WRGYHVITNPTEASRFTVANFIAGRSWLPATNVPFAAGL 523

BLAST of MELO3C006817 vs. NCBI nr
Match: gi|703116295|ref|XP_010101099.1| (putative pectinesterase/pectinesterase inhibitor 17 [Morus notabilis])

HSP 1 Score: 766.9 bits (1979), Expect = 2.3e-218
Identity = 381/525 (72.57%), Postives = 435/525 (82.86%), Query Frame = 1

Query: 8   TSLVGVF-LFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFF----NHGTKSPPKD 67
           T  +G+F LFL F  L S   S   SNGIDWWC +T YPETCK  +    NH     PK 
Sbjct: 2   TKNLGLFVLFLSFSSLLSLTLSSVHSNGIDWWCNRTPYPETCKQSWRDNDNHQKLFIPKL 61

Query: 68  MTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSS 127
             DFKK+ +Q ++++AL A+SH K +GSKCRN KEKAAW+DCLKLY++ IL LN+TLD S
Sbjct: 62  KADFKKLELQISLKQALKADSHNKWLGSKCRNDKEKAAWSDCLKLYEDAILLLNKTLDPS 121

Query: 128 TKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAA 187
           TK T+FD QTWLSTALTNL+TCR GF EL V D++WPL MS+NV+KLISN+LA NNASA 
Sbjct: 122 TKCTDFDAQTWLSTALTNLDTCRAGFVELGVSDFVWPL-MSNNVSKLISNTLATNNASAP 181

Query: 188 VGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSG 247
                  YK GFP WLS GDR+LLQS+S   +LVVA+DGSG++ T+ AAL+ AAKR  SG
Sbjct: 182 PVTN--AYKDGFPIWLSPGDRKLLQSSSQSPNLVVAKDGSGDHETIKAALDAAAKRSGSG 241

Query: 248 RFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGS-TTFNSATVAVTGE 307
           RF+I VKRGVY ENLEIG+K+KNIML+GDG+R+T ITG+RSVGGG  TTFNSATVAVTGE
Sbjct: 242 RFIIHVKRGVYNENLEIGNKLKNIMLVGDGLRYTIITGSRSVGGGGYTTFNSATVAVTGE 301

Query: 308 GFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 367
           GFIA+GITF+NTAGPENHQAVALRSGADLSVFYRC FEGYQDTLYVHSQRQFYKECYIYG
Sbjct: 302 GFIAQGITFRNTAGPENHQAVALRSGADLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYG 361

Query: 368 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKP 427
           TVDFIFGNAA VLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DLKP
Sbjct: 362 TVDFIFGNAAAVLQNCMIYARRPMSKQKNTITAQGRTDPNQNTGISIHNSRVMAAADLKP 421

Query: 428 VESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSP 487
           V S+ KTYLGRPWK+YSRTVFM+TY+DSLVDP GWLEWDGDFALNTLYYGEY N+GP + 
Sbjct: 422 VLSSFKTYLGRPWKQYSRTVFMQTYLDSLVDPAGWLEWDGDFALNTLYYGEYRNLGPAAS 481

Query: 488 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 527
            S+RVKW GY VI++S+EAS+FTV NFIAGQSWLP T V FT GL
Sbjct: 482 TSRRVKWSGYRVITSSSEASQFTVANFIAGQSWLPATGVRFTAGL 523