CmaCh04G003340 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh04G003340 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.1e-166
Identity = 293/488 (60.04%), Postives = 371/488 (76.02%), Query Frame = 1

Query: 64  WCTKTTYPETCKYFYGNANNPPP-KDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHK 123
           WC KT  P+ C+YF     +    K  ++F K++++ A++ A + +  T  +GS+CRN +
Sbjct: 31  WCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGSKCRNER 90

Query: 124 ERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDN 183
           E+ AW DC +LY+ T+ +L +T  SS   T+ D QTWLSTALTN+ETCR    +L V + 
Sbjct: 91  EKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLEDLGVPEY 150

Query: 184 VLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVA 243
           VLPL+ S NVTKLISN+L++N       + +YK GFP+W+  GDRKLLQ++    ++VVA
Sbjct: 151 VLPLL-SNNVTKLISNTLSLNKVP--YNEPSYKDGFPTWVKPGDRKLLQTTPR-ANIVVA 210

Query: 244 QDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFI 303
           QDGSG+ KT+  A+  AA R    R+VI +K G Y EN+E+  K+KNIM VGDG+  T I
Sbjct: 211 QDGSGNVKTIQEAVA-AASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTII 270

Query: 304 TSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAF 363
           T S+SVGGG+TTF SATVAV G+ FIA+ IT RNTAGP NHQAVALRSG+D SVFYRC+F
Sbjct: 271 TGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSF 330

Query: 364 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRT 423
           EGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVLQNC I+ARKP N + N +TAQGRT
Sbjct: 331 EGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPN-RTNTLTAQGRT 390

Query: 424 DPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLE 483
           DPNQ+TGI IHN RV A  DLK V+ +VKTFLGRPWK+YSRTV+IKT+LDSL++PAGW+E
Sbjct: 391 DPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWME 450

Query: 484 WNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGT 543
           W+GDFALNTLYY EY N GP SST  RV+W+GYHV+++ ++ S+F+V NFI+G SWLP T
Sbjct: 451 WSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 510

Query: 544 EVPFIPGI 551
            VPF  G+
Sbjct: 511 NVPFTSGL 510

BLAST of CmaCh04G003340 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 566.6 bits (1459), Expect = 2.9e-160
Identity = 285/513 (55.56%), Postives = 377/513 (73.49%), Query Frame = 1

Query: 39  VLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYG-NANNPPPKDMSEFKKMAI 98
           ++ F+   +  +S  S +    +  WC++T  P+ C+YF   N+NN P K  SEF K+++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 99  KFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQ 158
           K  +D A+  + H   +G +CR+ +E+ AW DC+KLY  T+ ++  T++ + K ++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 159 TWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQG 218
           TWLSTALTN++TCR GF EL V D VLPL+ S NV+ L+ N+LA+N    +      K G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPLM-SNNVSNLLCNTLAINKVP-FNYTPPEKDG 187

Query: 219 FPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVY 278
           FPSW+  GDRKLLQSS+   + VVA+DGSG+ KT+  A++ A+     GRFVI VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASG---SGRFVIYVKQGVY 247

Query: 279 EENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNT 338
            ENLEI  + KN+ML GDG+  T IT S+SVGGG+TTFNSATVA  G+GFIA+GITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 339 AGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 398
           AG  N QAVALRSG+D SVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA V
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 399 LQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRP 458
           LQNC I+AR+P   + N ITAQGR+DPNQNTGI IHNSRV A  DL+ V G+ KT+LGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 459 WKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHV 518
           W++YSRTVF+KT LDSL+DP GWLEW+G+FAL TL+Y E++N GP +ST  RV W G+ V
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

Query: 519 ISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           + +++EAS+F+V  F++G SW+P + VPF  G+
Sbjct: 488 LGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

BLAST of CmaCh04G003340 vs. Swiss-Prot
Match: PME59_ARATH (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana GN=PME59 PE=2 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 5.9e-153
Identity = 279/533 (52.35%), Postives = 364/533 (68.29%), Query Frame = 1

Query: 40  LFFLHFFL-------PSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANN-PPPKDMSE 99
           + FLH  L       P ++   +++++    WC KT YP+ CK ++ N N    P  +SE
Sbjct: 10  ILFLHLILLVLLCVHPLTTVADRNSTD----WCDKTPYPDPCKCYFKNHNGFQQPTQLSE 69

Query: 100 FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE----- 159
           F+ M ++ AMD A+S        G  C + K++   ADC+ LY +TI QL RTL      
Sbjct: 70  FRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPK 129

Query: 160 --SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNN 219
             ++   T+FD QTWLSTALTN ETCR G ++LNV D + P++++  ++ LISN LAVN 
Sbjct: 130 AGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNG 189

Query: 220 ASTWQGKE----TYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAA 279
           A    G +      ++GFP+WLS  D++LL++  +  +LVVA+DGSG   TV AA++ A 
Sbjct: 190 ALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQAAIDVAG 249

Query: 280 KRN-TDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSAT 339
           +R  T GRFVI VKRG+Y+EN+ +     +IMLVGDGMR T IT  RSV GG TT+NSAT
Sbjct: 250 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 309

Query: 340 VAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYK 399
             + G  FIA+GITFRNTAGP   QAVALRS +D S+FY+C+ EGYQDTL VHSQRQFY+
Sbjct: 310 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 369

Query: 400 ECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMA 459
           ECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ N ITAQGR DP QNTGISIHNSR++ 
Sbjct: 370 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 429

Query: 460 TDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEY 519
             DLK V GTVKT++GRPW ++SRTV ++TYLD++V P GW  W     F L+TL+Y EY
Sbjct: 430 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 489

Query: 520 KNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           KN GP SST  RV WKG+HV+  +++AS F+V  FI+G +WLP T +PF  G+
Sbjct: 490 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of CmaCh04G003340 vs. Swiss-Prot
Match: PME60_ARATH (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana GN=PME60 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-151
Identity = 281/523 (53.73%), Postives = 357/523 (68.26%), Query Frame = 1

Query: 37  LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCK-YFYGNANNPPPKDMSEFKKM 96
           L +L  L   L   +  +   S  I  WC KT YP  CK YF  ++    P  +SEF+ +
Sbjct: 14  LHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVL 73

Query: 97  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-----SSTK 156
            ++ AMD A+S           C + K++   ADC+ LY +T+ QL RTL+     +  +
Sbjct: 74  LVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRR 133

Query: 157 STEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQG 216
            T+FD QTWLSTALTN ETCR G ++LNV D   P++++  ++ LISN LAVN A    G
Sbjct: 134 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 193

Query: 217 KETY----KQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRN-TD 276
           K        +GFP+W+S  +R+LLQ  S+  +LVVA+DGSG  KTV AA++ A +R  T 
Sbjct: 194 KNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTS 253

Query: 277 GRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGE 336
           GRFVI VKRG+Y+ENL +     NIMLVGDGMR+T IT  RSV GG TT++SAT  + G 
Sbjct: 254 GRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGL 313

Query: 337 GFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 396
            FIA+GI F+NTAGP   QAVALRS +D S+FYRC+ EGYQDTL VHSQRQFY+ECYIYG
Sbjct: 314 HFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYG 373

Query: 397 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQ 456
           TVDFIFGNAAVV QNC+I  R P+ GQ N ITAQGRTD  QNTGISIHNS ++   DLK 
Sbjct: 374 TVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKP 433

Query: 457 VEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPR 516
           V  +VKT++GRPW  YSRTV +KTY+DS+V P GW  W     + L+TL+Y EYKNIGP 
Sbjct: 434 VVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPA 493

Query: 517 SSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPF 547
           SST  RV+WKG+HV+S +++AS FSV  FI+G +WLPG+ +PF
Sbjct: 494 SSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

BLAST of CmaCh04G003340 vs. Swiss-Prot
Match: PME33_ARATH (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana GN=PME33 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.9e-151
Identity = 274/514 (53.31%), Postives = 355/514 (69.07%), Query Frame = 1

Query: 58  SNGIHWWCTKTTYPETCKYFY-----GNANNPPPKDMSEFKKMAIKFAMDGALSVERHTK 117
           S  + WWC KT + ETC Y++      N N  PP+  SEF +M +K A+D A+     T 
Sbjct: 81  SGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTV 140

Query: 118 RVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-------SSTKSTEFDIQTWLSTALT 177
           + G  C N++ + AW+DC+ L+QNT+ QL RTL+       S  K T+FD QTWLSTA T
Sbjct: 141 KFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQT 200

Query: 178 NVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKET----YKQGFPSW 237
           N+ETCR+G  +LNV D V+P+I++KN++ LI N LAVN     Q   T    +K+ FPSW
Sbjct: 201 NIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSW 260

Query: 238 LSGGDRKLLQSSSMMVD---LVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYE 297
           +S  +R+LL S+S+      LVVAQD SG  +++ AA+  AA+R    RFVI VK+GVY 
Sbjct: 261 VSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYR 320

Query: 298 ENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTA 357
           EN+++GN   NIMLVGDG R T ITS RSV  G TT+NSAT    G+ F+A+ +TF NTA
Sbjct: 321 ENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTA 380

Query: 358 GPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 417
           GP   QAVA+RS +D SVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIFGNAAVV 
Sbjct: 381 GPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVF 440

Query: 418 QNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPW 477
           QNCMI  R+P++GQ N ITAQGR DP QNTGI+IH+SR++A  DLK V    KT+LGRPW
Sbjct: 441 QNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPW 500

Query: 478 KEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYH 537
           + YSR   +KTY+D+ + P GW  W    +FALNT++YGEYKN GP SST  RV+WKG+H
Sbjct: 501 QAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFH 560

Query: 538 VISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            I+++  AS F+V + I+G SWLP T VPF  G+
Sbjct: 561 AITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of CmaCh04G003340 vs. TrEMBL
Match: A0A0A0L9G4_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G175720 PE=4 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 1.3e-255
Identity = 450/549 (81.97%), Postives = 483/549 (87.98%), Query Frame = 1

Query: 7   LLP--YITKPLRATLALINTYNELGLSMERSILGVLFFLHFFL-PSSSAFSKHASNGIHW 66
           LLP  YI K  R+ LA  N     GLSM  S  GV  FLHFFL  SSSAFSK ASNGI W
Sbjct: 25  LLPLYYIIKHKRSILASSNI-EMAGLSMGTSF-GVFIFLHFFLLSSSSAFSKPASNGIDW 84

Query: 67  WCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKE 126
           WC KTTYPETCKYF+ +    PPKDM++FKKM I+FAM+ ALS E HTK VGS+CRN KE
Sbjct: 85  WCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKE 144

Query: 127 RVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNV 186
           + AWADCLKLYQNTI QL +TL+SSTKSTEFDIQTWLSTALTN+ETCRTGFAELNV D +
Sbjct: 145 KAAWADCLKLYQNTILQLNQTLDSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYI 204

Query: 187 LPLITSKNVTKLISNSLAVNNASTW--QGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVV 246
           LPLI S NVT+LISNSLA+NNAS     GKETYK+GFPSWLSGGDR+LLQSSS  VDLVV
Sbjct: 205 LPLIMSDNVTELISNSLAINNASAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVV 264

Query: 247 AQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTF 306
           AQDGSG++ TV AALEEAAKR T GRFVIQVKRGVY ENLEIG+KMKNIML+GDGMRFTF
Sbjct: 265 AQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTF 324

Query: 307 ITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCA 366
           IT +RSVGGGSTTFNSATVAVTGEGFIA+GITFRNTAGPENHQAVALRSGAD SVFYRCA
Sbjct: 325 ITGNRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCA 384

Query: 367 FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGR 426
           FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNA+TAQGR
Sbjct: 385 FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGR 444

Query: 427 TDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWL 486
           TDPNQNTGISIHNSRVMATDDLK VE TVKT+LGRPWKEYSRTVF+KTY+DSLVDPAGWL
Sbjct: 445 TDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWL 504

Query: 487 EWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPG 546
           EW+GDFALNTLYYGEY NIGP S   QRV+WKGYHVI+N TEASEF+VQNFI+GQSWLP 
Sbjct: 505 EWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPD 564

Query: 547 TEVPFIPGI 551
           TEVPF PG+
Sbjct: 565 TEVPFTPGL 571

BLAST of CmaCh04G003340 vs. TrEMBL
Match: A0A067FQH6_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g010103mg PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-209
Identity = 369/524 (70.42%), Postives = 436/524 (83.21%), Query Frame = 1

Query: 30  LSMERSI-LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFY--GNANNPPP 89
           ++M+ S+ L  +  + FF P   A S  ASNG+ +WC+KT +PE CKYF    + +   P
Sbjct: 1   MAMKLSVFLLFISLISFFAP---ALSSVASNGVDYWCSKTPHPEPCKYFMQQNSKHFAVP 60

Query: 90  KDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE 149
           K  SEF++MA+  A+D AL+ + H K +GS+CRNHKE+VAWADCLKLYQ+TI QL  TL+
Sbjct: 61  KQKSEFRRMAMSLALDRALTAQNHNKWLGSKCRNHKEKVAWADCLKLYQDTINQLNHTLD 120

Query: 150 SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNAS 209
           S+TK T+FD QTWLSTALTN+ETCR GF EL V D VLPL+ S NVTKLISN+LA+ NAS
Sbjct: 121 SNTKCTDFDAQTWLSTALTNLETCRAGFVELGVPDYVLPLM-SNNVTKLISNTLALRNAS 180

Query: 210 TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDG 269
           T    ETYK GFPSW+  GDRKLLQ+S +  +LVVAQDGSG+++T+ AAL+ AAKR   G
Sbjct: 181 TVP--ETYKGGFPSWVKPGDRKLLQTSPVRPNLVVAQDGSGNYRTIKAALDAAAKRTGSG 240

Query: 270 RFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEG 329
           RFVI VKRGVY+ENLEIGNKMKNIMLVGDG+R+T IT SRSVGGG TTFNSATVAVTG+G
Sbjct: 241 RFVIHVKRGVYKENLEIGNKMKNIMLVGDGLRYTIITGSRSVGGGFTTFNSATVAVTGDG 300

Query: 330 FIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 389
           FIA+GITFRNTAGP+NHQAVALR+G+D SVFY+C FEGYQDTLYVHSQRQFYKECYIYGT
Sbjct: 301 FIARGITFRNTAGPQNHQAVALRAGSDLSVFYQCGFEGYQDTLYVHSQRQFYKECYIYGT 360

Query: 390 VDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQV 449
           VDFIFGNAAVVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DL  V
Sbjct: 361 VDFIFGNAAVVLQNCMIYARRPMDKQKNVVTAQGRTDPNQNTGISIHNSRVMAAPDLVPV 420

Query: 450 EGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSST 509
               KTFLGRPWKEYSRTV+++TYLDSLVDPAGWLEW+G+FALNTL+YGEYKNIGP +ST
Sbjct: 421 LSKFKTFLGRPWKEYSRTVYMQTYLDSLVDPAGWLEWSGNFALNTLFYGEYKNIGPAAST 480

Query: 510 DQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
             RV+W+GY VI+++TEAS+F+V +FI+G SWLP T VPF  G+
Sbjct: 481 SGRVKWRGYRVITSATEASKFTVGSFITGNSWLPATGVPFRSGL 518

BLAST of CmaCh04G003340 vs. TrEMBL
Match: V4S7X2_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10004742mg PE=4 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.4e-209
Identity = 369/524 (70.42%), Postives = 436/524 (83.21%), Query Frame = 1

Query: 30  LSMERSI-LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFY--GNANNPPP 89
           ++M+ S+ L  +  + FF P   A S  ASNG+ +WC+KT +PE CKYF    + +   P
Sbjct: 1   MAMKLSVFLLFISLISFFAP---AVSSVASNGVDYWCSKTPHPEPCKYFMQQNSKHFAVP 60

Query: 90  KDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE 149
           K  SEF++MA+  A+D AL+ + H K +GS+CRNHKE+VAWADCLKLYQ+TI QL  TL+
Sbjct: 61  KQESEFRRMAMSLALDRALTAQNHNKWLGSKCRNHKEKVAWADCLKLYQDTINQLNHTLD 120

Query: 150 SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNAS 209
           S+TK T+FD QTWLSTALTN+ETCR GF EL V D VLPL+ S NVTKLISN+LA+ NAS
Sbjct: 121 SNTKCTDFDAQTWLSTALTNLETCRAGFVELGVPDYVLPLM-SNNVTKLISNTLALRNAS 180

Query: 210 TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDG 269
           T    ETYK GFPSW+  GDRKLLQ+S +  +LVVAQDGSG+++T+ AAL+ AAKR   G
Sbjct: 181 TVP--ETYKGGFPSWVKPGDRKLLQTSPVRPNLVVAQDGSGNYRTIKAALDAAAKRTGSG 240

Query: 270 RFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEG 329
           RFVI VKRGVY+ENLEIGNKMKNIMLVGDG+R+T IT SRSVGGG TTFNSATVAVTG+G
Sbjct: 241 RFVIHVKRGVYKENLEIGNKMKNIMLVGDGLRYTIITGSRSVGGGFTTFNSATVAVTGDG 300

Query: 330 FIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 389
           FIA+GITFRNTAGP+NHQAVALR+G+D SVFY+C FEGYQDTLYVHSQRQFYKECYIYGT
Sbjct: 301 FIARGITFRNTAGPQNHQAVALRAGSDLSVFYQCGFEGYQDTLYVHSQRQFYKECYIYGT 360

Query: 390 VDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQV 449
           VDFIFGNAAVVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DL  V
Sbjct: 361 VDFIFGNAAVVLQNCMIYARRPMDKQKNVVTAQGRTDPNQNTGISIHNSRVMAAPDLVPV 420

Query: 450 EGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSST 509
               KTFLGRPWKEYSRTV+++TYLDSLVDPAGWLEW+G+FALNTL+YGEYKNIGP +ST
Sbjct: 421 LSKFKTFLGRPWKEYSRTVYMQTYLDSLVDPAGWLEWSGNFALNTLFYGEYKNIGPAAST 480

Query: 510 DQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
             RV+W+GY VI+++TEAS+F+V +FI+G SWLP T VPF  G+
Sbjct: 481 SGRVKWRGYRVITSATEASKFTVGSFIAGNSWLPATGVPFRSGL 518

BLAST of CmaCh04G003340 vs. TrEMBL
Match: A0A068VJK2_COFCA (Pectinesterase OS=Coffea canephora GN=GSCOC_T00009515001 PE=4 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 1.5e-208
Identity = 365/520 (70.19%), Postives = 432/520 (83.08%), Query Frame = 1

Query: 37  LGVLFFLHFFLPS--SSAFSKHASNG-IHWWCTKTTYPETCKYFYGN--ANNPPPKDMSE 96
           +G LFF    L S  S  FSK+ S+G I+WWC +T YPE C YF+ N   N+  PK+ ++
Sbjct: 5   IGWLFFTFISLSSFFSPCFSKNPSSGDINWWCNQTPYPEQCNYFFLNHGTNHFMPKEKAD 64

Query: 97  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 156
            KKMA++  ++ AL  + HT+ + S+CRN +E+ AWADCLKLY+NTI QL RTL+S+TK 
Sbjct: 65  VKKMAMQITLERALKAKTHTENLESKCRNEREKAAWADCLKLYENTILQLNRTLDSNTKC 124

Query: 157 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAV-NNASTWQG 216
           T+FD QTWLSTALTN+ETCR+GF EL V D VLPL+ S NV+KLI N+LA+ NN +    
Sbjct: 125 TDFDAQTWLSTALTNLETCRSGFQELGVSDFVLPLM-SNNVSKLICNTLALNNNINVSVE 184

Query: 217 KETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVI 276
            +TYK GFP+W++ GDRKLLQSS +  +L+VAQDGSG+++T+ AAL+ AAKR+  GRFVI
Sbjct: 185 TQTYKDGFPTWVTPGDRKLLQSSLVRPNLIVAQDGSGNYRTIKAALDAAAKRSGSGRFVI 244

Query: 277 QVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQ 336
           QVKRG Y ENLEIGNKMKNIML+G GMR T IT SRSVGGGSTTF+SATVAVTG+GFIAQ
Sbjct: 245 QVKRGTYRENLEIGNKMKNIMLLGAGMRDTIITGSRSVGGGSTTFDSATVAVTGDGFIAQ 304

Query: 337 GITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFI 396
           GITFRNTAGP+NHQAVALRSG+D SVF+RC FEGYQDTLYVHSQRQFYKECYIYGTVDFI
Sbjct: 305 GITFRNTAGPQNHQAVALRSGSDLSVFHRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFI 364

Query: 397 FGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTV 456
           FGNAAVVLQNCMIYAR+PM+ QKN ITAQGRTDPNQNTGISIHNSRVMA  DL+ V  + 
Sbjct: 365 FGNAAVVLQNCMIYARRPMDKQKNTITAQGRTDPNQNTGISIHNSRVMAASDLRPVLSSF 424

Query: 457 KTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRV 516
           KT+LGRPWK+YSRTVFI+TYLDSLVDPAGWLEW+G+FA NTLYYGEYKN GP SST +RV
Sbjct: 425 KTYLGRPWKQYSRTVFIQTYLDSLVDPAGWLEWDGNFAFNTLYYGEYKNSGPGSSTSRRV 484

Query: 517 QWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           +W+GYHVI+N TEAS F+V NFI+G+SWLP T VPF  G+
Sbjct: 485 KWRGYHVITNPTEASRFTVANFIAGRSWLPATNVPFAAGL 523

BLAST of CmaCh04G003340 vs. TrEMBL
Match: A0A061G787_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_014942 PE=4 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.7e-207
Identity = 362/515 (70.29%), Postives = 425/515 (82.52%), Query Frame = 1

Query: 37  LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNP-PPKDMSEFKKM 96
           L V+F     +P +   S   +NGI +WC +T  P+ CKYF         PK  SEF+KM
Sbjct: 7   LFVIFMSFSLVPPT--LSSVVTNGIDYWCDRTPNPQPCKYFMRQKGKSYVPKQKSEFRKM 66

Query: 97  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFD 156
           A++ A+D AL+ + H K +G +CRN KE+ AWADCLKLY+ TI QL +TL+S TK T+FD
Sbjct: 67  AVQIALDRALNAQSHNKGLGKKCRNDKEKAAWADCLKLYEETILQLNQTLDS-TKCTDFD 126

Query: 157 IQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKETYK 216
           +QTWLSTALTN+ETCR GF EL V D +LPL+ S NV+KLISN+LA+NN +++   +TYK
Sbjct: 127 VQTWLSTALTNLETCRAGFVELGVSDYILPLM-SNNVSKLISNTLALNNNASFV-PQTYK 186

Query: 217 QGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRG 276
            GFP WL  GDRKLLQSSS   +LVVAQDGSG+H+T+  AL+ AAKR+  GRFVI VKRG
Sbjct: 187 HGFPRWLKAGDRKLLQSSSPTPNLVVAQDGSGNHRTIKEALDAAAKRSGSGRFVIHVKRG 246

Query: 277 VYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFR 336
           VY+ENLEIGNK+KNIMLVGDG+R+T IT SRSVGGGSTTFNSATVAVTGEGFIA+GITFR
Sbjct: 247 VYKENLEIGNKLKNIMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFR 306

Query: 337 NTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 396
           NTAGP+NHQAVALRSG+D SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA
Sbjct: 307 NTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 366

Query: 397 VVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLG 456
           VVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA +DL  V  + KTFLG
Sbjct: 367 VVLQNCMIYARRPMDKQKNIVTAQGRTDPNQNTGISIHNSRVMAANDLTPVLSSFKTFLG 426

Query: 457 RPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGY 516
           RPWKEYSRTVF++TYLDSLVDPAGW EW+GDFALNTLYYGEYKNIGP + T +RV WKGY
Sbjct: 427 RPWKEYSRTVFMQTYLDSLVDPAGWFEWDGDFALNTLYYGEYKNIGPAAPTSRRVTWKGY 486

Query: 517 HVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            VI+++TEAS+F+V NFI+G+SWLP T +PF  G+
Sbjct: 487 RVITSATEASKFTVTNFIAGRSWLPDTGIPFTSGL 516

BLAST of CmaCh04G003340 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 566.6 bits (1459), Expect = 1.7e-161
Identity = 285/513 (55.56%), Postives = 377/513 (73.49%), Query Frame = 1

Query: 39  VLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYG-NANNPPPKDMSEFKKMAI 98
           ++ F+   +  +S  S +    +  WC++T  P+ C+YF   N+NN P K  SEF K+++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 99  KFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQ 158
           K  +D A+  + H   +G +CR+ +E+ AW DC+KLY  T+ ++  T++ + K ++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 159 TWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQG 218
           TWLSTALTN++TCR GF EL V D VLPL+ S NV+ L+ N+LA+N    +      K G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPLM-SNNVSNLLCNTLAINKVP-FNYTPPEKDG 187

Query: 219 FPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVY 278
           FPSW+  GDRKLLQSS+   + VVA+DGSG+ KT+  A++ A+     GRFVI VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASG---SGRFVIYVKQGVY 247

Query: 279 EENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNT 338
            ENLEI  + KN+ML GDG+  T IT S+SVGGG+TTFNSATVA  G+GFIA+GITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 339 AGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 398
           AG  N QAVALRSG+D SVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA V
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 399 LQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRP 458
           LQNC I+AR+P   + N ITAQGR+DPNQNTGI IHNSRV A  DL+ V G+ KT+LGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 459 WKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHV 518
           W++YSRTVF+KT LDSL+DP GWLEW+G+FAL TL+Y E++N GP +ST  RV W G+ V
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

Query: 519 ISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           + +++EAS+F+V  F++G SW+P + VPF  G+
Sbjct: 488 LGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

BLAST of CmaCh04G003340 vs. TAIR10
Match: AT5G51490.1 (AT5G51490.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 542.3 bits (1396), Expect = 3.3e-154
Identity = 279/533 (52.35%), Postives = 364/533 (68.29%), Query Frame = 1

Query: 40  LFFLHFFL-------PSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANN-PPPKDMSE 99
           + FLH  L       P ++   +++++    WC KT YP+ CK ++ N N    P  +SE
Sbjct: 10  ILFLHLILLVLLCVHPLTTVADRNSTD----WCDKTPYPDPCKCYFKNHNGFQQPTQLSE 69

Query: 100 FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE----- 159
           F+ M ++ AMD A+S        G  C + K++   ADC+ LY +TI QL RTL      
Sbjct: 70  FRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPK 129

Query: 160 --SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNN 219
             ++   T+FD QTWLSTALTN ETCR G ++LNV D + P++++  ++ LISN LAVN 
Sbjct: 130 AGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNG 189

Query: 220 ASTWQGKE----TYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAA 279
           A    G +      ++GFP+WLS  D++LL++  +  +LVVA+DGSG   TV AA++ A 
Sbjct: 190 ALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA--VRANLVVAKDGSGHFNTVQAAIDVAG 249

Query: 280 KRN-TDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSAT 339
           +R  T GRFVI VKRG+Y+EN+ +     +IMLVGDGMR T IT  RSV GG TT+NSAT
Sbjct: 250 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 309

Query: 340 VAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYK 399
             + G  FIA+GITFRNTAGP   QAVALRS +D S+FY+C+ EGYQDTL VHSQRQFY+
Sbjct: 310 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 369

Query: 400 ECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMA 459
           ECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ N ITAQGR DP QNTGISIHNSR++ 
Sbjct: 370 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 429

Query: 460 TDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEY 519
             DLK V GTVKT++GRPW ++SRTV ++TYLD++V P GW  W     F L+TL+Y EY
Sbjct: 430 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 489

Query: 520 KNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           KN GP SST  RV WKG+HV+  +++AS F+V  FI+G +WLP T +PF  G+
Sbjct: 490 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of CmaCh04G003340 vs. TAIR10
Match: AT5G51500.1 (AT5G51500.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 537.7 bits (1384), Expect = 8.2e-153
Identity = 281/523 (53.73%), Postives = 357/523 (68.26%), Query Frame = 1

Query: 37  LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCK-YFYGNANNPPPKDMSEFKKM 96
           L +L  L   L   +  +   S  I  WC KT YP  CK YF  ++    P  +SEF+ +
Sbjct: 14  LHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVL 73

Query: 97  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-----SSTK 156
            ++ AMD A+S           C + K++   ADC+ LY +T+ QL RTL+     +  +
Sbjct: 74  LVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRR 133

Query: 157 STEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQG 216
            T+FD QTWLSTALTN ETCR G ++LNV D   P++++  ++ LISN LAVN A    G
Sbjct: 134 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 193

Query: 217 KETY----KQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRN-TD 276
           K        +GFP+W+S  +R+LLQ  S+  +LVVA+DGSG  KTV AA++ A +R  T 
Sbjct: 194 KNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTS 253

Query: 277 GRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGE 336
           GRFVI VKRG+Y+ENL +     NIMLVGDGMR+T IT  RSV GG TT++SAT  + G 
Sbjct: 254 GRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGL 313

Query: 337 GFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 396
            FIA+GI F+NTAGP   QAVALRS +D S+FYRC+ EGYQDTL VHSQRQFY+ECYIYG
Sbjct: 314 HFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYG 373

Query: 397 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQ 456
           TVDFIFGNAAVV QNC+I  R P+ GQ N ITAQGRTD  QNTGISIHNS ++   DLK 
Sbjct: 374 TVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKP 433

Query: 457 VEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPR 516
           V  +VKT++GRPW  YSRTV +KTY+DS+V P GW  W     + L+TL+Y EYKNIGP 
Sbjct: 434 VVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPA 493

Query: 517 SSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPF 547
           SST  RV+WKG+HV+S +++AS FSV  FI+G +WLPG+ +PF
Sbjct: 494 SSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

BLAST of CmaCh04G003340 vs. TAIR10
Match: AT3G47400.1 (AT3G47400.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-152
Identity = 274/514 (53.31%), Postives = 355/514 (69.07%), Query Frame = 1

Query: 58  SNGIHWWCTKTTYPETCKYFY-----GNANNPPPKDMSEFKKMAIKFAMDGALSVERHTK 117
           S  + WWC KT + ETC Y++      N N  PP+  SEF +M +K A+D A+     T 
Sbjct: 81  SGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTV 140

Query: 118 RVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-------SSTKSTEFDIQTWLSTALT 177
           + G  C N++ + AW+DC+ L+QNT+ QL RTL+       S  K T+FD QTWLSTA T
Sbjct: 141 KFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQT 200

Query: 178 NVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKET----YKQGFPSW 237
           N+ETCR+G  +LNV D V+P+I++KN++ LI N LAVN     Q   T    +K+ FPSW
Sbjct: 201 NIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSW 260

Query: 238 LSGGDRKLLQSSSMMVD---LVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYE 297
           +S  +R+LL S+S+      LVVAQD SG  +++ AA+  AA+R    RFVI VK+GVY 
Sbjct: 261 VSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYR 320

Query: 298 ENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTA 357
           EN+++GN   NIMLVGDG R T ITS RSV  G TT+NSAT    G+ F+A+ +TF NTA
Sbjct: 321 ENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTA 380

Query: 358 GPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 417
           GP   QAVA+RS +D SVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIFGNAAVV 
Sbjct: 381 GPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVF 440

Query: 418 QNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPW 477
           QNCMI  R+P++GQ N ITAQGR DP QNTGI+IH+SR++A  DLK V    KT+LGRPW
Sbjct: 441 QNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPW 500

Query: 478 KEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYH 537
           + YSR   +KTY+D+ + P GW  W    +FALNT++YGEYKN GP SST  RV+WKG+H
Sbjct: 501 QAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFH 560

Query: 538 VISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            I+++  AS F+V + I+G SWLP T VPF  G+
Sbjct: 561 AITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of CmaCh04G003340 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-120
Identity = 237/484 (48.97%), Postives = 313/484 (64.67%), Query Frame = 1

Query: 65  CTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKER 124
           C +T +P  CK       + P +  ++F +++++  +DGA+  +  T  +  +  + +  
Sbjct: 8   CDETPHPGECKTLL--IKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGSKQ-- 67

Query: 125 VAWADCLKLYQNTIFQLKRT-LESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNV 184
            AW +C+ LY+ TI +L  + L      +  D+Q WLSTALTN++TC+   +EL V  + 
Sbjct: 68  -AWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVSSHS 127

Query: 185 LPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQ 244
           L  IT       + N+LA+N      GKE         +S    K L S    VD+VVAQ
Sbjct: 128 LESITID-----VINTLAINKRMEQNGKEF-------GISKITMKTL-SIGEKVDVVVAQ 187

Query: 245 DGSGDHKTVAAALEEAAKR-NTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFI 304
           DGSGD+KT+  A+  A +R     R+VI VK+GVYEE + +G K  NIM+ GDG+  T I
Sbjct: 188 DGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIGKTII 247

Query: 305 TSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAF 364
           T  +S G G +T+ SAT    G+GF+ + IT RNTAGPENHQAVALRS +D SVFYRC+ 
Sbjct: 248 TGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSDMSVFYRCSI 307

Query: 365 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRT 424
           EGYQDTLYVHS RQF++EC IYGTVDFIFGNAA VLQNC I+AR P NG  N ITAQ R 
Sbjct: 308 EGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNG-VNTITAQSRF 367

Query: 425 DPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLE 484
           +PNQ TGI IHNS V     ++   G VKT+LGRPW+ Y+RTV I TYLD+L++P GW++
Sbjct: 368 NPNQTTGIVIHNSVVKGAPGVQL--GGVKTYLGRPWRSYARTVVIGTYLDTLIEPNGWID 427

Query: 485 WNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGT 544
           W+   AL+TLYYGEY+N GP S T+ RV W G+HVIS+  EA EF++  FI   SWLP T
Sbjct: 428 WDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSASWLPPT 470

Query: 545 EVPF 547
           +VPF
Sbjct: 488 KVPF 470

BLAST of CmaCh04G003340 vs. NCBI nr
Match: gi|659077230|ref|XP_008439095.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 901.0 bits (2327), Expect = 1.0e-258
Identity = 449/548 (81.93%), Postives = 485/548 (88.50%), Query Frame = 1

Query: 7   LLP--YITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWW 66
           LLP  YI KP R+ LA  N     GLSME S++GV  FLHFFL SSSAFSK ASNGI WW
Sbjct: 25  LLPLYYIIKPKRSILASSNQ-EMAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWW 84

Query: 67  CTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKER 126
           C KTTYPETCKYF+ +    PPKDM++FKKM I+FAM+ ALS E HTK VGS+CRN KE+
Sbjct: 85  CKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEK 144

Query: 127 VAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVL 186
            AWADCLKLYQNTI QL +TL+SSTK TEFDIQTWLSTALTN+ETCRTGFAELNV+D + 
Sbjct: 145 AAWADCLKLYQNTILQLNQTLDSSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIW 204

Query: 187 PLITSKNVTKLISNSLAVNNAST--WQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVA 246
           PLI S NVTKLISNSLA+NNAS     GK TYK+GFPSWLSGGDR+LLQS+S  VDLVVA
Sbjct: 205 PLIMSDNVTKLISNSLAINNASAAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVA 264

Query: 247 QDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFI 306
           QDGSG++ TV AALEEAAKR T GRFVIQVKRGVY ENLEIG+KMKNIML+GDGMRFTFI
Sbjct: 265 QDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFI 324

Query: 307 TSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAF 366
           T +RSVGGGSTTFNSATVAVTGEGFIA+GITF+NTAGPENHQAVALRSGAD SVFYRCAF
Sbjct: 325 TGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAF 384

Query: 367 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRT 426
           EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNA+TAQGRT
Sbjct: 385 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRT 444

Query: 427 DPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLE 486
           DPNQNTGISIHNSRVMATDDLK VE TVKT+LGRPWKEYSRTVF+KTY+DSLVDP GWLE
Sbjct: 445 DPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLE 504

Query: 487 WNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGT 546
           W+GDFALNTLYYGEY NIGP S   QRV+WKGYHVISNSTEASEF+VQNFI+GQSWLP T
Sbjct: 505 WDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNT 564

Query: 547 EVPFIPGI 551
           EVPF PG+
Sbjct: 565 EVPFTPGL 571

BLAST of CmaCh04G003340 vs. NCBI nr
Match: gi|778679320|ref|XP_004148139.2| (PREDICTED: pectinesterase 2 [Cucumis sativus])

HSP 1 Score: 890.2 bits (2299), Expect = 1.8e-255
Identity = 450/549 (81.97%), Postives = 483/549 (87.98%), Query Frame = 1

Query: 7   LLP--YITKPLRATLALINTYNELGLSMERSILGVLFFLHFFL-PSSSAFSKHASNGIHW 66
           LLP  YI K  R+ LA  N     GLSM  S  GV  FLHFFL  SSSAFSK ASNGI W
Sbjct: 25  LLPLYYIIKHKRSILASSNI-EMAGLSMGTSF-GVFIFLHFFLLSSSSAFSKPASNGIDW 84

Query: 67  WCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKE 126
           WC KTTYPETCKYF+ +    PPKDM++FKKM I+FAM+ ALS E HTK VGS+CRN KE
Sbjct: 85  WCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKE 144

Query: 127 RVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNV 186
           + AWADCLKLYQNTI QL +TL+SSTKSTEFDIQTWLSTALTN+ETCRTGFAELNV D +
Sbjct: 145 KAAWADCLKLYQNTILQLNQTLDSSTKSTEFDIQTWLSTALTNLETCRTGFAELNVSDYI 204

Query: 187 LPLITSKNVTKLISNSLAVNNASTW--QGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVV 246
           LPLI S NVT+LISNSLA+NNAS     GKETYK+GFPSWLSGGDR+LLQSSS  VDLVV
Sbjct: 205 LPLIMSDNVTELISNSLAINNASAGVGNGKETYKKGFPSWLSGGDRRLLQSSSTKVDLVV 264

Query: 247 AQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTF 306
           AQDGSG++ TV AALEEAAKR T GRFVIQVKRGVY ENLEIG+KMKNIML+GDGMRFTF
Sbjct: 265 AQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTF 324

Query: 307 ITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCA 366
           IT +RSVGGGSTTFNSATVAVTGEGFIA+GITFRNTAGPENHQAVALRSGAD SVFYRCA
Sbjct: 325 ITGNRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADLSVFYRCA 384

Query: 367 FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGR 426
           FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNA+TAQGR
Sbjct: 385 FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGR 444

Query: 427 TDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWL 486
           TDPNQNTGISIHNSRVMATDDLK VE TVKT+LGRPWKEYSRTVF+KTY+DSLVDPAGWL
Sbjct: 445 TDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWL 504

Query: 487 EWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPG 546
           EW+GDFALNTLYYGEY NIGP S   QRV+WKGYHVI+N TEASEF+VQNFI+GQSWLP 
Sbjct: 505 EWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVITNLTEASEFTVQNFIAGQSWLPD 564

Query: 547 TEVPFIPGI 551
           TEVPF PG+
Sbjct: 565 TEVPFTPGL 571

BLAST of CmaCh04G003340 vs. NCBI nr
Match: gi|641846563|gb|KDO65446.1| (hypothetical protein CISIN_1g010103mg [Citrus sinensis])

HSP 1 Score: 737.3 bits (1902), Expect = 2.0e-209
Identity = 369/524 (70.42%), Postives = 436/524 (83.21%), Query Frame = 1

Query: 30  LSMERSI-LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFY--GNANNPPP 89
           ++M+ S+ L  +  + FF P   A S  ASNG+ +WC+KT +PE CKYF    + +   P
Sbjct: 1   MAMKLSVFLLFISLISFFAP---ALSSVASNGVDYWCSKTPHPEPCKYFMQQNSKHFAVP 60

Query: 90  KDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE 149
           K  SEF++MA+  A+D AL+ + H K +GS+CRNHKE+VAWADCLKLYQ+TI QL  TL+
Sbjct: 61  KQKSEFRRMAMSLALDRALTAQNHNKWLGSKCRNHKEKVAWADCLKLYQDTINQLNHTLD 120

Query: 150 SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNAS 209
           S+TK T+FD QTWLSTALTN+ETCR GF EL V D VLPL+ S NVTKLISN+LA+ NAS
Sbjct: 121 SNTKCTDFDAQTWLSTALTNLETCRAGFVELGVPDYVLPLM-SNNVTKLISNTLALRNAS 180

Query: 210 TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDG 269
           T    ETYK GFPSW+  GDRKLLQ+S +  +LVVAQDGSG+++T+ AAL+ AAKR   G
Sbjct: 181 TVP--ETYKGGFPSWVKPGDRKLLQTSPVRPNLVVAQDGSGNYRTIKAALDAAAKRTGSG 240

Query: 270 RFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEG 329
           RFVI VKRGVY+ENLEIGNKMKNIMLVGDG+R+T IT SRSVGGG TTFNSATVAVTG+G
Sbjct: 241 RFVIHVKRGVYKENLEIGNKMKNIMLVGDGLRYTIITGSRSVGGGFTTFNSATVAVTGDG 300

Query: 330 FIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 389
           FIA+GITFRNTAGP+NHQAVALR+G+D SVFY+C FEGYQDTLYVHSQRQFYKECYIYGT
Sbjct: 301 FIARGITFRNTAGPQNHQAVALRAGSDLSVFYQCGFEGYQDTLYVHSQRQFYKECYIYGT 360

Query: 390 VDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQV 449
           VDFIFGNAAVVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DL  V
Sbjct: 361 VDFIFGNAAVVLQNCMIYARRPMDKQKNVVTAQGRTDPNQNTGISIHNSRVMAAPDLVPV 420

Query: 450 EGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSST 509
               KTFLGRPWKEYSRTV+++TYLDSLVDPAGWLEW+G+FALNTL+YGEYKNIGP +ST
Sbjct: 421 LSKFKTFLGRPWKEYSRTVYMQTYLDSLVDPAGWLEWSGNFALNTLFYGEYKNIGPAAST 480

Query: 510 DQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
             RV+W+GY VI+++TEAS+F+V +FI+G SWLP T VPF  G+
Sbjct: 481 SGRVKWRGYRVITSATEASKFTVGSFITGNSWLPATGVPFRSGL 518

BLAST of CmaCh04G003340 vs. NCBI nr
Match: gi|567858024|ref|XP_006421695.1| (hypothetical protein CICLE_v10004742mg [Citrus clementina])

HSP 1 Score: 736.5 bits (1900), Expect = 3.4e-209
Identity = 369/524 (70.42%), Postives = 436/524 (83.21%), Query Frame = 1

Query: 30  LSMERSI-LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFY--GNANNPPP 89
           ++M+ S+ L  +  + FF P   A S  ASNG+ +WC+KT +PE CKYF    + +   P
Sbjct: 1   MAMKLSVFLLFISLISFFAP---AVSSVASNGVDYWCSKTPHPEPCKYFMQQNSKHFAVP 60

Query: 90  KDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE 149
           K  SEF++MA+  A+D AL+ + H K +GS+CRNHKE+VAWADCLKLYQ+TI QL  TL+
Sbjct: 61  KQESEFRRMAMSLALDRALTAQNHNKWLGSKCRNHKEKVAWADCLKLYQDTINQLNHTLD 120

Query: 150 SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNAS 209
           S+TK T+FD QTWLSTALTN+ETCR GF EL V D VLPL+ S NVTKLISN+LA+ NAS
Sbjct: 121 SNTKCTDFDAQTWLSTALTNLETCRAGFVELGVPDYVLPLM-SNNVTKLISNTLALRNAS 180

Query: 210 TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDG 269
           T    ETYK GFPSW+  GDRKLLQ+S +  +LVVAQDGSG+++T+ AAL+ AAKR   G
Sbjct: 181 TVP--ETYKGGFPSWVKPGDRKLLQTSPVRPNLVVAQDGSGNYRTIKAALDAAAKRTGSG 240

Query: 270 RFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEG 329
           RFVI VKRGVY+ENLEIGNKMKNIMLVGDG+R+T IT SRSVGGG TTFNSATVAVTG+G
Sbjct: 241 RFVIHVKRGVYKENLEIGNKMKNIMLVGDGLRYTIITGSRSVGGGFTTFNSATVAVTGDG 300

Query: 330 FIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 389
           FIA+GITFRNTAGP+NHQAVALR+G+D SVFY+C FEGYQDTLYVHSQRQFYKECYIYGT
Sbjct: 301 FIARGITFRNTAGPQNHQAVALRAGSDLSVFYQCGFEGYQDTLYVHSQRQFYKECYIYGT 360

Query: 390 VDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQV 449
           VDFIFGNAAVVLQNCMIYAR+PM+ QKN +TAQGRTDPNQNTGISIHNSRVMA  DL  V
Sbjct: 361 VDFIFGNAAVVLQNCMIYARRPMDKQKNVVTAQGRTDPNQNTGISIHNSRVMAAPDLVPV 420

Query: 450 EGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSST 509
               KTFLGRPWKEYSRTV+++TYLDSLVDPAGWLEW+G+FALNTL+YGEYKNIGP +ST
Sbjct: 421 LSKFKTFLGRPWKEYSRTVYMQTYLDSLVDPAGWLEWSGNFALNTLFYGEYKNIGPAAST 480

Query: 510 DQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
             RV+W+GY VI+++TEAS+F+V +FI+G SWLP T VPF  G+
Sbjct: 481 SGRVKWRGYRVITSATEASKFTVGSFIAGNSWLPATGVPFRSGL 518

BLAST of CmaCh04G003340 vs. NCBI nr
Match: gi|661873590|emb|CDP20891.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 733.8 bits (1893), Expect = 2.2e-208
Identity = 365/520 (70.19%), Postives = 432/520 (83.08%), Query Frame = 1

Query: 37  LGVLFFLHFFLPS--SSAFSKHASNG-IHWWCTKTTYPETCKYFYGN--ANNPPPKDMSE 96
           +G LFF    L S  S  FSK+ S+G I+WWC +T YPE C YF+ N   N+  PK+ ++
Sbjct: 5   IGWLFFTFISLSSFFSPCFSKNPSSGDINWWCNQTPYPEQCNYFFLNHGTNHFMPKEKAD 64

Query: 97  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 156
            KKMA++  ++ AL  + HT+ + S+CRN +E+ AWADCLKLY+NTI QL RTL+S+TK 
Sbjct: 65  VKKMAMQITLERALKAKTHTENLESKCRNEREKAAWADCLKLYENTILQLNRTLDSNTKC 124

Query: 157 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAV-NNASTWQG 216
           T+FD QTWLSTALTN+ETCR+GF EL V D VLPL+ S NV+KLI N+LA+ NN +    
Sbjct: 125 TDFDAQTWLSTALTNLETCRSGFQELGVSDFVLPLM-SNNVSKLICNTLALNNNINVSVE 184

Query: 217 KETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVI 276
            +TYK GFP+W++ GDRKLLQSS +  +L+VAQDGSG+++T+ AAL+ AAKR+  GRFVI
Sbjct: 185 TQTYKDGFPTWVTPGDRKLLQSSLVRPNLIVAQDGSGNYRTIKAALDAAAKRSGSGRFVI 244

Query: 277 QVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQ 336
           QVKRG Y ENLEIGNKMKNIML+G GMR T IT SRSVGGGSTTF+SATVAVTG+GFIAQ
Sbjct: 245 QVKRGTYRENLEIGNKMKNIMLLGAGMRDTIITGSRSVGGGSTTFDSATVAVTGDGFIAQ 304

Query: 337 GITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFI 396
           GITFRNTAGP+NHQAVALRSG+D SVF+RC FEGYQDTLYVHSQRQFYKECYIYGTVDFI
Sbjct: 305 GITFRNTAGPQNHQAVALRSGSDLSVFHRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFI 364

Query: 397 FGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTV 456
           FGNAAVVLQNCMIYAR+PM+ QKN ITAQGRTDPNQNTGISIHNSRVMA  DL+ V  + 
Sbjct: 365 FGNAAVVLQNCMIYARRPMDKQKNTITAQGRTDPNQNTGISIHNSRVMAASDLRPVLSSF 424

Query: 457 KTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRV 516
           KT+LGRPWK+YSRTVFI+TYLDSLVDPAGWLEW+G+FA NTLYYGEYKN GP SST +RV
Sbjct: 425 KTYLGRPWKQYSRTVFIQTYLDSLVDPAGWLEWDGNFAFNTLYYGEYKNSGPGSSTSRRV 484

Query: 517 QWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           +W+GYHVI+N TEAS F+V NFI+G+SWLP T VPF  G+
Sbjct: 485 KWRGYHVITNPTEASRFTVANFIAGRSWLPATNVPFAAGL 523