MELO3C006788 (gene) Melon (DHL92) v3.5.1

Overview
NameMELO3C006788
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionDNA topoisomerase
Locationchr6 : 5970755 .. 5973059 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGCATGGAATAGGCACGGATGCATCAATACCCGTACACATTAACAACATAGGCGAGCGCAACTATGTTCAGGTTAGTTATGTCCTGCTGGCTGATATGATAGTCTTTGGCATGGAAAATTGCACGCTGTTTGGTTATTTAGTTACATTGTTATGTTGCAGGTTCAAGCTGGGAGGAGGTTGGTTCCAACTAACTTGGGCATCACCTTAATTAGAGGATATCAATGTATTGATCCAGATCTCTGTTTACCAGATATTCGAAGTTTCATCGAGCATCAGATCACTCTTGTTGCGAAAGGTCAAGCAGATCATTCTAGTGTTGTGGAGAGTGTATTGCAACAGTTCAAGCAAAAGTATAGCTACTTCGTTAAGAAGGTTATTTTCCCCTGTGGCTTACTTACTAGTGGACATATCGTTATCAATGAAGCATTAGAGACTTTTCGTTTTTTTCTTTTTCTTTTTTGTTAAATAAATTTATTATTAGTTAAATTCAAACTTTTTCTTATTTACTTGTAACTAGTCATGTTCTTTATTCACTTATTGGTTATTTTGGTGCAAAATCATTCCAGACATCCAATAAGGCTTTCCTCTTTTAGAATTTTAATGCATAGCATCTGGAACCAGGCTTTTTAATTTCTCGATTGGTAAAATTGCTTCTTATCTTGTTCAGATTGATCGTATGGATGCTCTATTTGAAGCACAATTTTCTCCCCTTGCCGATTCAGGACGTGCTTTGAGCAAATGTGGAAAGTGCCTACGGTATATGAAGTTTATTTCTTCCCAACCACAGCGTCTGTACTGTGGTATATGTGAGGAGGTTTATTATCTTCCTCAAAAGGGAACAATAAAGGTAAATATCCCACAAAATAGTTCCAATTCCTTCCATTGATTGCATTGACTCTTCAGCTTTCTTTGTTTCACGTTCTGTATATTGTCGGTGGGATATTTTCTTAATGTACGCTCACTTTTTGAAAGAATGTGGTAACTGCTTCCAGTCTGAGTTACCTTGTCGACTATTTAGTAATTCTTATGTATTGCTGCTTTTACGTGCAATATTTGGAATATAAGCATTTGCAGAAATTGTTGCATGCCCTCAGCTTCATTTTGTTCCATTTCTTCTTTCTGATTGCACGATTTATGAAGGATTCGCTTTCTTTTTAAGTAGTAGCCATGATTACTAATTTGTTTAAATGCTGCTTTTATGGTTGATGGCTGAAGTGCCCCTAAACAACTTGTTACACTGTGGTTGATGACTTATGAATGTTTCTAAGATTGCTTGGTTAAATATAAGAGCTCTCTGATTCTACAACGAAGTAACTCATCGTTCTTTTTCAGCTGTACAAGGAGCTTACATGCCCTCTGGACAACTTCGAGCTCTTGATTCTTTCCTTACCTGGCCCAGAAGGCAAGTCATTTCCTCTTTGCCCATATTGCTACAATAGTCCTCCATTTGAGGGTATCGACACATTATATGGTGCTTCTAAAACCAACCCAAGTTCCGGAACATCAAGCAAGGGAATGGGCATGCCATGCTTCCTGTGTCCTCACCCAACATGCCGACATTCTTTAATAACCCAAGGAGTCTGCGCTTGCCCAGAATGTAGCGGGACGCTTGTCCTAGATCCTGTAAGTGCACCCAACTGGAAACTCAACTGCAACAAATGCAATTGTCTTGTCTTCCTTCCTCAAGGTGCTCATAGGATTTCCACCACAAGTGACAAGTGTGCTGATTGTGACTCTACAATTATAGAAGTTGACTTCAACAAGAAAACGACACCATTGGAGGATGGAGCTACATTGTTTGTTGGATGCATTCTTTGCGATGAGTTTCTCCATTCACTTCTGGAGATGAAGCATGGAAATTCATTTATCAGAAACAGATTTTCGAGAGGAAGAGGAAGAGGAAGGGGAAGAGGACGGGGAAGGGGAGGAAGAGGACGTAATTCAGGGAAACCAATGGACCCAAAAATGAGTTTCCGAGACTTCTGAAGAAATGCAGACTAGCATTTAGAAATTTTAGAGGAAGTGCATGAAATTTTTTGGAAGGAAACGCGAAGAGATGAGCCACCTACACCTTTCCTTATACTTAATTTGGCCTTTAGCTTGGATGGTGTTAGCTCTTGTGTTCCTTAGGTTGGGAAGCTGCTGACCAACATCTTTGTTTTCTTCTTGTTTTTCCTTATGTAGTCAAAATTTTGAACACTAGCGTGTTTTTATTACCCTCTAATATAATGAAGAAATGTGGAATGATACCTGCCATTCAATCTTTTATGAAATATCCTGAATTTGTAGATTATATG

mRNA sequence

ATGGAGAAGCATGGAATAGGCACGGATGCATCAATACCCGTACACATTAACAACATAGGCGAGCGCAACTATGTTCAGGTTCAAGCTGGGAGGAGGTTGGTTCCAACTAACTTGGGCATCACCTTAATTAGAGGATATCAATGTATTGATCCAGATCTCTGTTTACCAGATATTCGAAGTTTCATCGAGCATCAGATCACTCTTGTTGCGAAAGGTCAAGCAGATCATTCTAGTGTTGTGGAGAGTGTATTGCAACAGTTCAAGCAAAAGTATAGCTACTTCGTTAAGAAGATTGATCGTATGGATGCTCTATTTGAAGCACAATTTTCTCCCCTTGCCGATTCAGGACGTGCTTTGAGCAAATGTGGAAAGTGCCTACGGTATATGAAGTTTATTTCTTCCCAACCACAGCGTCTGTACTGTGGTATATGTGAGGAGGTTTATTATCTTCCTCAAAAGGGAACAATAAAGCTGTACAAGGAGCTTACATGCCCTCTGGACAACTTCGAGCTCTTGATTCTTTCCTTACCTGGCCCAGAAGGCAAGTCATTTCCTCTTTGCCCATATTGCTACAATAGTCCTCCATTTGAGGGTATCGACACATTATATGGTGCTTCTAAAACCAACCCAAGTTCCGGAACATCAAGCAAGGGAATGGGCATGCCATGCTTCCTGTGTCCTCACCCAACATGCCGACATTCTTTAATAACCCAAGGAGTCTGCGCTTGCCCAGAATGTAGCGGGACGCTTGTCCTAGATCCTGTAAGTGCACCCAACTGGAAACTCAACTGCAACAAATGCAATTGTCTTGTCTTCCTTCCTCAAGGTGCTCATAGGATTTCCACCACAAGTGACAAGTGTGCTGATTGTGACTCTACAATTATAGAAGTTGACTTCAACAAGAAAACGACACCATTGGAGGATGGAGCTACATTGTTTGTTGGATGCATTCTTTGCGATGAGTTTCTCCATTCACTTCTGGAGATGAAGCATGGAAATTCATTTATCAGAAACAGATTTTCGAGAGGAAGAGGAAGAGGAAGGGGAAGAGGACGGGGAAGGGGAGGAAGAGGACGTAATTCAGGGAAACCAATGGACCCAAAAATGAGTTTCCGAGACTTCTGAAGAAATGCAGACTAGCATTTAGAAATTTTAGAGGAAGTGCATGAAATTTTTTGGAAGGAAACGCGAAGAGATGAGCCACCTACACCTTTCCTTATACTTAATTTGGCCTTTAGCTTGGATGGTGTTAGCTCTTGTGTTCCTTAGGTTGGGAAGCTGCTGACCAACATCTTTGTTTTCTTCTTGTTTTTCCTTATGTAGTCAAAATTTTGAACACTAGCGTGTTTTTATTACCCTCTAATATAATGAAGAAATGTGGAATGATACCTGCCATTCAATCTTTTATGAAATATCCTGAATTTGTAGATTATATG

Coding sequence (CDS)

ATGGAGAAGCATGGAATAGGCACGGATGCATCAATACCCGTACACATTAACAACATAGGCGAGCGCAACTATGTTCAGGTTCAAGCTGGGAGGAGGTTGGTTCCAACTAACTTGGGCATCACCTTAATTAGAGGATATCAATGTATTGATCCAGATCTCTGTTTACCAGATATTCGAAGTTTCATCGAGCATCAGATCACTCTTGTTGCGAAAGGTCAAGCAGATCATTCTAGTGTTGTGGAGAGTGTATTGCAACAGTTCAAGCAAAAGTATAGCTACTTCGTTAAGAAGATTGATCGTATGGATGCTCTATTTGAAGCACAATTTTCTCCCCTTGCCGATTCAGGACGTGCTTTGAGCAAATGTGGAAAGTGCCTACGGTATATGAAGTTTATTTCTTCCCAACCACAGCGTCTGTACTGTGGTATATGTGAGGAGGTTTATTATCTTCCTCAAAAGGGAACAATAAAGCTGTACAAGGAGCTTACATGCCCTCTGGACAACTTCGAGCTCTTGATTCTTTCCTTACCTGGCCCAGAAGGCAAGTCATTTCCTCTTTGCCCATATTGCTACAATAGTCCTCCATTTGAGGGTATCGACACATTATATGGTGCTTCTAAAACCAACCCAAGTTCCGGAACATCAAGCAAGGGAATGGGCATGCCATGCTTCCTGTGTCCTCACCCAACATGCCGACATTCTTTAATAACCCAAGGAGTCTGCGCTTGCCCAGAATGTAGCGGGACGCTTGTCCTAGATCCTGTAAGTGCACCCAACTGGAAACTCAACTGCAACAAATGCAATTGTCTTGTCTTCCTTCCTCAAGGTGCTCATAGGATTTCCACCACAAGTGACAAGTGTGCTGATTGTGACTCTACAATTATAGAAGTTGACTTCAACAAGAAAACGACACCATTGGAGGATGGAGCTACATTGTTTGTTGGATGCATTCTTTGCGATGAGTTTCTCCATTCACTTCTGGAGATGAAGCATGGAAATTCATTTATCAGAAACAGATTTTCGAGAGGAAGAGGAAGAGGAAGGGGAAGAGGACGGGGAAGGGGAGGAAGAGGACGTAATTCAGGGAAACCAATGGACCCAAAAATGAGTTTCCGAGACTTCTGA

Protein sequence

MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF*
Homology
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BLAST of MELO3C006788 vs. Swiss-Prot
Match: TOP3B_ARATH (DNA topoisomerase 3-beta OS=Arabidopsis thaliana GN=At2g32000 PE=2 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 1.2e-178
Identity = 293/374 (78.34%), Postives = 327/374 (87.43%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 497 MEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCIDPDLCLPDIRS 556

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIE QITLVAKGQADHS VV+ V+QQF++K+SYFV++I+ MDALFEAQFSPLADSGRALS
Sbjct: 557 FIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFSPLADSGRALS 616

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYKELTCPLDNFEL+I S+PGPE
Sbjct: 617 KCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFELVIYSVPGPE 676

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGIDTL+GASKT  +   +  G GMPC LCPHPTC+HS+  QGV
Sbjct: 677 GKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPTCQHSVRNQGV 736

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TTS++C +CDS IIE+DFN
Sbjct: 737 CACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPECDSAIIEIDFN 796

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLE+GATL  GC+LCDE L SL+E+KHG SF+R     GRGRGRGRGRGRG  GR 
Sbjct: 797 KKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVRR---GGRGRGRGRGRGRG--GRR 856

Query: 361 SGKPMDPKMSFRDF 375
             K +DPKMSFRDF
Sbjct: 857 GSKSVDPKMSFRDF 865

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BLAST of MELO3C006788 vs. Swiss-Prot
Match: TOP3B_ORYSJ (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica GN=TOP3B PE=2 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.1e-169
Identity = 282/374 (75.40%), Postives = 311/374 (83.16%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEK+GIGTDASIPVH+NNI ERNYVQV +GRRLVPT LG TLIRGYQCID DLCLPDIR 
Sbjct: 495 MEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGYQCIDADLCLPDIRR 554

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIE QITL+AKG+ADH  VV+ VLQQF +KYSYFVKKI+ MDALFEAQFSPLADSGR LS
Sbjct: 555 FIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFEAQFSPLADSGRLLS 614

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKC RYMK+IS+QP RLYC  CEEVYYLPQ G+IKLYKE+ CPLD FELL+ S+ GP+
Sbjct: 615 KCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPLDGFELLLFSMVGPD 674

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
            KSFPLCP+CYNSPPFEGID L+GA K +  +G   KG GMPCFLC HPTC+ S+ITQGV
Sbjct: 675 AKSFPLCPFCYNSPPFEGIDKLFGALKLD-DTGKVGKGAGMPCFLCLHPTCKQSMITQGV 734

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPEC+GTL+LDPVSAP W+L CN+CNC+V LP  AH+ISTT  KC  C+STIIEVDFN
Sbjct: 735 CACPECTGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKKCPTCESTIIEVDFN 794

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPL+DGATL  GCILCDE LHSL+EMKHG SF   R  RGRGRGRGRGRG     R 
Sbjct: 795 KKTTPLKDGATLHEGCILCDELLHSLIEMKHGKSFFMRR-GRGRGRGRGRGRGSSRGRRG 854

Query: 361 SGKPMDPKMSFRDF 375
           S +  DPKMSFRDF
Sbjct: 855 SSRHDDPKMSFRDF 866

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BLAST of MELO3C006788 vs. Swiss-Prot
Match: TOP3B_MOUSE (DNA topoisomerase 3-beta-1 OS=Mus musculus GN=Top3b PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.4e-105
Identity = 193/372 (51.88%), Postives = 241/372 (64.78%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLGI L+ GY  ID +L LP IRS
Sbjct: 503 MEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRS 562

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
            +E Q+ L+A+G+AD+  V+   L  FK+K+ YFV  I  MD L E  FSPLA +G+ LS
Sbjct: 563 AVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLS 622

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           +CGKC R+MK+I ++P RL+C  C+E Y LPQ GTIKLYKEL CPLD+FEL++ S  G  
Sbjct: 623 RCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWS-SGSR 682

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKS+PLCPYCYN PPF  +                 KGMG  C  C HPTC+HSL   G+
Sbjct: 683 GKSYPLCPYCYNHPPFRDM----------------KKGMG--CNECTHPTCQHSLSMLGI 742

Query: 241 CACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDF 300
             C EC +G LVLDP S P WK+ CN CN +    + AHR+  ++D C  C++ +++VDF
Sbjct: 743 GQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVSADTCNTCEAALLDVDF 802

Query: 301 NKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNS-FIRNRFSRGRGRGRGRGRGRGGRG 360
           NK  +PL    T   GCI CD     L+E+KH  S    +R   GR +GRGRGRGR   G
Sbjct: 803 NKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRGRRPPG 855

Query: 361 RNSGKPMDPKMS 371
           + + +    KMS
Sbjct: 863 KPNPRRPKDKMS 855

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BLAST of MELO3C006788 vs. Swiss-Prot
Match: TOP3B_HUMAN (DNA topoisomerase 3-beta-1 OS=Homo sapiens GN=TOP3B PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.4e-105
Identity = 192/372 (51.61%), Postives = 243/372 (65.32%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLGI L+ GY  ID +L LP IRS
Sbjct: 503 MEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRS 562

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
            +E Q+ L+A+G+AD+  V+   L  FK+K+ YFV  I  MD L E  FSPLA +G+ LS
Sbjct: 563 AVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLS 622

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           +CGKC R+MK+I ++P RL+C  C+E Y LPQ GTIKLYKEL CPLD+FEL++ S  G  
Sbjct: 623 RCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWS-SGSR 682

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKS+PLCPYCYN PPF  +                 KGMG  C  C HP+C+HSL   G+
Sbjct: 683 GKSYPLCPYCYNHPPFRDM----------------KKGMG--CNECTHPSCQHSLSMLGI 742

Query: 241 CACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDF 300
             C EC SG LVLDP S P WK+ CNKCN +    + AHR+  ++D C+ C++ +++VDF
Sbjct: 743 GQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDF 802

Query: 301 NKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNS-FIRNRFSRGRGRGRGRGRGRGGRG 360
           NK  +PL    T  +GC+ CD     L+E+KH  S    +R   GR +GRGRGR R   G
Sbjct: 803 NKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPG 855

Query: 361 RNSGKPMDPKMS 371
           + + +    KMS
Sbjct: 863 KPNPRRPKDKMS 855

[Close]
BLAST of MELO3C006788 vs. Swiss-Prot
Match: TOP3B_DROME (DNA topoisomerase 3-beta OS=Drosophila melanogaster GN=Top3beta PE=2 SV=2)

HSP 1 Score: 329.7 bits (844), Expect = 4.1e-89
Identity = 169/359 (47.08%), Postives = 222/359 (61.84%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           ME+HGIGTDASIPVHINNI +RNYV ++ GR+L+PT LGI L+ GYQ IDP+L LP +R+
Sbjct: 498 MEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRT 557

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
            +E  +TL+A+G A+   V+   ++ FK K+ YFVK ID MDALFE  FSPLA+SG+A S
Sbjct: 558 EVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHS 617

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           +CGKC RYMK+I ++P RL+C  C+E Y LP  G +K+Y+E  CPLD+F+LL  S  G +
Sbjct: 618 RCGKCRRYMKYIQTKPARLHCSHCDETYALP-IGNVKVYREFKCPLDDFDLLAFS-TGVK 677

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           G+S+P CPYCYN PPF  +  L G                  C  C +  C HSL T G+
Sbjct: 678 GRSYPFCPYCYNHPPFSDMPHLGG------------------CNTCTNANCPHSLNTLGI 737

Query: 241 CACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDF 300
            +C EC +G LVLD   AP WKL CN+C+ ++   +GA +I+    KC +C +  + V +
Sbjct: 738 SSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVY 797

Query: 301 NKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRG 359
               +  +DG+    GCI C      L+E KH     R   S G  RG   GRG GG G
Sbjct: 798 KSDKSKFKDGSEEKSGCIFCSADFSHLVE-KHRAVASRPVRSGGGFRGGKAGRGGGGMG 835

[Close]
BLAST of MELO3C006788 vs. TrEMBL
Match: A0A0A0L9C7_CUCSA (DNA topoisomerase OS=Cucumis sativus GN=Csa_3G172970 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.2e-215
Identity = 360/374 (96.26%), Postives = 366/374 (97.86%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS
Sbjct: 496 MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 555

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALFEAQFSPLADSGRALS
Sbjct: 556 FIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFSPLADSGRALS 615

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLI SLPGPE
Sbjct: 616 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLIYSLPGPE 675

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+CPHPTCRHSLITQGV
Sbjct: 676 GKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPTCRHSLITQGV 735

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT DKCADCDSTIIEVDFN
Sbjct: 736 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADCDSTIIEVDFN 795

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S  RGRGRGRGRGRGGRGR+
Sbjct: 796 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS--RGRGRGRGRGRGGRGRS 855

Query: 361 SGKPMDPKMSFRDF 375
           SGKPMDPKMSFRDF
Sbjct: 856 SGKPMDPKMSFRDF 867

[Close]
BLAST of MELO3C006788 vs. TrEMBL
Match: A0A0D2T8F3_GOSRA (DNA topoisomerase OS=Gossypium raimondii GN=B456_011G150100 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 1.5e-186
Identity = 311/377 (82.49%), Postives = 341/377 (90.45%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEK+GIGTDASIPVHINNI ERNYVQVQAGRRLVPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 497 MEKNGIGTDASIPVHINNICERNYVQVQAGRRLVPTTLGITLIRGYQCIDPDLCLPDIRS 556

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQA+H  VV+ VLQQFKQK+ YF+++I+ MDALFEAQFSPLADSGRALS
Sbjct: 557 FIEHQITLVAKGQANHFRVVQHVLQQFKQKFCYFLQQIENMDALFEAQFSPLADSGRALS 616

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK+IS+QPQR++CG CEEVYYLPQKGTIKLYKELTCPLDNFELLI S+PGPE
Sbjct: 617 KCGKCLRYMKYISAQPQRMFCGTCEEVYYLPQKGTIKLYKELTCPLDNFELLIFSMPGPE 676

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGIDTL+G++K   S+G   KG GMPCFLC HPTCRHS++ QGV
Sbjct: 677 GKSFPLCPYCYNSPPFEGIDTLFGSAKIG-STGKLGKGAGMPCFLCLHPTCRHSVVAQGV 736

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TT D+CA+CDSTIIEVDFN
Sbjct: 737 CACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRDRCAECDSTIIEVDFN 796

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRG---GR 360
           KKTTPLEDGATL  GCILCDE LHSL+E+KHG SF R   +RGRGRGRG GRGRG   GR
Sbjct: 797 KKTTPLEDGATLHTGCILCDELLHSLVEVKHGKSFGRGFGARGRGRGRGTGRGRGGYRGR 856

Query: 361 GRNSGKPMDPKMSFRDF 375
           GR +GK +DP+MSFRDF
Sbjct: 857 GRTAGKKVDPRMSFRDF 872

[Close]
BLAST of MELO3C006788 vs. TrEMBL
Match: W9R2T8_9ROSA (DNA topoisomerase OS=Morus notabilis GN=L484_019920 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 1.6e-185
Identity = 310/374 (82.89%), Postives = 336/374 (89.84%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEK+GIGTDASIPVH+NNI ERNYV+VQAGR+LVPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 270 MEKYGIGTDASIPVHVNNICERNYVKVQAGRKLVPTPLGITLIRGYQCIDPDLCLPDIRS 329

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIE Q+TLVAKGQADH  V++ VLQQFKQK+SYFVK+ID+MDALFEAQFSP+A+SGR LS
Sbjct: 330 FIEQQLTLVAKGQADHICVLQHVLQQFKQKFSYFVKQIDKMDALFEAQFSPVANSGRVLS 389

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK+ISSQP RLYC  CEE+YYLPQKGTIKLYKELTCPLDNFELLI S PGPE
Sbjct: 390 KCGKCLRYMKYISSQPSRLYCNTCEEIYYLPQKGTIKLYKELTCPLDNFELLIFSRPGPE 449

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYN+PPFEGIDTL+G +KT  S     +G+GMPC LCPHPTC+HS+ITQGV
Sbjct: 450 GKSFPLCPYCYNTPPFEGIDTLFGGAKTG-SLNKLGEGVGMPCSLCPHPTCQHSMITQGV 509

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAP W+LNCN CNCLVFLPQGAHRISTT D+C DCDSTIIEVDFN
Sbjct: 510 CACPECSGTLVLDPVSAPKWRLNCNTCNCLVFLPQGAHRISTTRDQCPDCDSTIIEVDFN 569

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLEDGATL+ GCILCDEFLHSLLEMKHG SF R   SRGRGRG GR  G  GRGR 
Sbjct: 570 KKTTPLEDGATLYTGCILCDEFLHSLLEMKHGKSFFRRLSSRGRGRGTGRD-GPRGRGRG 629

Query: 361 SGKPMDPKMSFRDF 375
           +GK +DPKMSFRDF
Sbjct: 630 TGKWVDPKMSFRDF 641

[Close]
BLAST of MELO3C006788 vs. TrEMBL
Match: A0A061EL32_THECC (DNA topoisomerase OS=Theobroma cacao GN=TCM_020175 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 2.2e-182
Identity = 305/374 (81.55%), Postives = 335/374 (89.57%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           ME++ IGTDASIPVHINNI ERNYVQVQ GR+LVPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 497 MERNRIGTDASIPVHINNICERNYVQVQGGRKLVPTALGITLIRGYQCIDPDLCLPDIRS 556

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQA+HS VV+ VLQQFKQK+SYFV++I+ MDALFEAQFSPLADSGRALS
Sbjct: 557 FIEHQITLVAKGQANHSHVVQHVLQQFKQKFSYFVQQIENMDALFEAQFSPLADSGRALS 616

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK+IS+QP RL+CG CEEVY+LPQKGTIKLYKELTCPLDNFELLI S+PGPE
Sbjct: 617 KCGKCLRYMKYISTQPPRLFCGTCEEVYHLPQKGTIKLYKELTCPLDNFELLIFSMPGPE 676

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEG+DTL+GA++   SSG   KG GMPCFLCPHPTCRHS++ QGV
Sbjct: 677 GKSFPLCPYCYNSPPFEGMDTLFGATRIG-SSGKLGKGAGMPCFLCPHPTCRHSVVAQGV 736

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TT ++CA+CDSTIIEVDFN
Sbjct: 737 CACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRERCAECDSTIIEVDFN 796

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLEDGATL  GCILCDE LHS +E+KHG SF R    RGRGRG  RG G  GRGR 
Sbjct: 797 KKTTPLEDGATLHTGCILCDELLHSFVEVKHGKSFSRGLGGRGRGRGTRRG-GYRGRGRA 856

Query: 361 SGKPMDPKMSFRDF 375
           +GK +DPKMSFRDF
Sbjct: 857 AGKQVDPKMSFRDF 868

[Close]
BLAST of MELO3C006788 vs. TrEMBL
Match: A0A0K9RGT7_SPIOL (DNA topoisomerase OS=Spinacia oleracea GN=SOVF_068130 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 4.9e-182
Identity = 301/374 (80.48%), Postives = 328/374 (87.70%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNI ERNYVQVQ+GRRLVPT LGITL+RGYQC DPDLCLPDIRS
Sbjct: 495 MEKHGIGTDASIPVHINNICERNYVQVQSGRRLVPTALGITLVRGYQCTDPDLCLPDIRS 554

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQADHS VV+ VL+QFKQKY YFVK+I+ MDALFEAQFSPL++SGRALS
Sbjct: 555 FIEHQITLVAKGQADHSLVVKHVLEQFKQKYVYFVKQIEHMDALFEAQFSPLSESGRALS 614

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKC RYMK+IS QPQRLYCG CE+VYYLPQKG+IKLYKE+ CPLDNFELL+ S+PGPE
Sbjct: 615 KCGKCARYMKYISQQPQRLYCGSCEDVYYLPQKGSIKLYKEIVCPLDNFELLLFSMPGPE 674

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYN+PPFEG+D L+GA KT P+     KG GMPCFLCPHPTC HSLI QGV
Sbjct: 675 GKSFPLCPYCYNNPPFEGVDKLFGAPKTGPAK--LGKGAGMPCFLCPHPTCPHSLIAQGV 734

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAP W+L+CN CNCLVFLPQGAHRISTT ++C DC+STI+EVDFN
Sbjct: 735 CACPECSGTLVLDPVSAPKWRLDCNMCNCLVFLPQGAHRISTTKERCPDCESTIVEVDFN 794

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           K  TPL DG TL+ GCILCDE LHSL+EMKHG S  R    RGRGRGRGRGRGRGGR   
Sbjct: 795 KNNTPLADGTTLYAGCILCDELLHSLIEMKHGKSSFRRFGGRGRGRGRGRGRGRGGR--- 854

Query: 361 SGKPMDPKMSFRDF 375
             K +DPKMSFRDF
Sbjct: 855 --KNVDPKMSFRDF 861

[Close]
BLAST of MELO3C006788 vs. TAIR10
Match: AT2G32000.1 (AT2G32000.1 DNA topoisomerase, type IA, core)

HSP 1 Score: 627.1 bits (1616), Expect = 7.0e-180
Identity = 293/374 (78.34%), Postives = 327/374 (87.43%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 497 MEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCIDPDLCLPDIRS 556

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIE QITLVAKGQADHS VV+ V+QQF++K+SYFV++I+ MDALFEAQFSPLADSGRALS
Sbjct: 557 FIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFSPLADSGRALS 616

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYKELTCPLDNFEL+I S+PGPE
Sbjct: 617 KCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFELVIYSVPGPE 676

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGIDTL+GASKT  +   +  G GMPC LCPHPTC+HS+  QGV
Sbjct: 677 GKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPTCQHSVRNQGV 736

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TTS++C +CDS IIE+DFN
Sbjct: 737 CACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPECDSAIIEIDFN 796

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLE+GATL  GC+LCDE L SL+E+KHG SF+R     GRGRGRGRGRGRG  GR 
Sbjct: 797 KKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVRR---GGRGRGRGRGRGRG--GRR 856

Query: 361 SGKPMDPKMSFRDF 375
             K +DPKMSFRDF
Sbjct: 857 GSKSVDPKMSFRDF 865

[Close]
BLAST of MELO3C006788 vs. TAIR10
Match: AT5G63920.1 (AT5G63920.1 topoisomerase 3alpha)

HSP 1 Score: 72.0 bits (175), Expect = 8.7e-13
Identity = 45/133 (33.83%), Postives = 67/133 (50.38%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           M+K GIGTDA++  HI  + +R Y    A  R  PTNLG  L+ GY  +  +L  P++R+
Sbjct: 513 MDKAGIGTDATMHDHIKKLLDRGYATKDANTRFSPTNLGEALVMGYDDMGYELWKPNLRA 572

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKY-SYFVKK---IDRMDALFE----AQFSPL 120
            +EH +  V+ G+   + V+E+ LQQ K  +    VKK   ++ M   FE       S  
Sbjct: 573 LMEHDMNEVSVGRKTKAEVLETCLQQMKACFLDARVKKSKLLEAMTIFFERSNNTDESES 632

Query: 121 ADSGRALSKCGKC 126
             +G  + +C  C
Sbjct: 633 QTAGEVVRRCNLC 645

[Close]
BLAST of MELO3C006788 vs. NCBI nr
Match: gi|659077152|ref|XP_008439059.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Cucumis melo])

HSP 1 Score: 788.1 bits (2034), Expect = 6.7e-225
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS
Sbjct: 496 MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 555

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS
Sbjct: 556 FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 615

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE
Sbjct: 616 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 675

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV
Sbjct: 676 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 735

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN
Sbjct: 736 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 795

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN
Sbjct: 796 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 855

Query: 361 SGKPMDPKMSFRDF 375
           SGKPMDPKMSFRDF
Sbjct: 856 SGKPMDPKMSFRDF 869

[Close]
BLAST of MELO3C006788 vs. NCBI nr
Match: gi|449459882|ref|XP_004147675.1| (PREDICTED: DNA topoisomerase 3-beta isoform X1 [Cucumis sativus])

HSP 1 Score: 756.9 bits (1953), Expect = 1.7e-215
Identity = 360/374 (96.26%), Postives = 366/374 (97.86%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS
Sbjct: 496 MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 555

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALFEAQFSPLADSGRALS
Sbjct: 556 FIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFSPLADSGRALS 615

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLI SLPGPE
Sbjct: 616 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLIYSLPGPE 675

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+CPHPTCRHSLITQGV
Sbjct: 676 GKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPTCRHSLITQGV 735

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT DKCADCDSTIIEVDFN
Sbjct: 736 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADCDSTIIEVDFN 795

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S  RGRGRGRGRGRGGRGR+
Sbjct: 796 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS--RGRGRGRGRGRGGRGRS 855

Query: 361 SGKPMDPKMSFRDF 375
           SGKPMDPKMSFRDF
Sbjct: 856 SGKPMDPKMSFRDF 867

[Close]
BLAST of MELO3C006788 vs. NCBI nr
Match: gi|778679257|ref|XP_011651110.1| (PREDICTED: DNA topoisomerase 3-beta isoform X2 [Cucumis sativus])

HSP 1 Score: 756.9 bits (1953), Expect = 1.7e-215
Identity = 360/374 (96.26%), Postives = 366/374 (97.86%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS
Sbjct: 436 MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 495

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALFEAQFSPLADSGRALS
Sbjct: 496 FIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFSPLADSGRALS 555

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLI SLPGPE
Sbjct: 556 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLIYSLPGPE 615

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+CPHPTCRHSLITQGV
Sbjct: 616 GKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPTCRHSLITQGV 675

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT DKCADCDSTIIEVDFN
Sbjct: 676 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADCDSTIIEVDFN 735

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRGGRGRN 360
           KKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S  RGRGRGRGRGRGGRGR+
Sbjct: 736 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS--RGRGRGRGRGRGGRGRS 795

Query: 361 SGKPMDPKMSFRDF 375
           SGKPMDPKMSFRDF
Sbjct: 796 SGKPMDPKMSFRDF 807

[Close]
BLAST of MELO3C006788 vs. NCBI nr
Match: gi|823245872|ref|XP_012455603.1| (PREDICTED: DNA topoisomerase 3-beta isoform X1 [Gossypium raimondii])

HSP 1 Score: 660.2 bits (1702), Expect = 2.1e-186
Identity = 311/377 (82.49%), Postives = 341/377 (90.45%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEK+GIGTDASIPVHINNI ERNYVQVQAGRRLVPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 497 MEKNGIGTDASIPVHINNICERNYVQVQAGRRLVPTTLGITLIRGYQCIDPDLCLPDIRS 556

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQA+H  VV+ VLQQFKQK+ YF+++I+ MDALFEAQFSPLADSGRALS
Sbjct: 557 FIEHQITLVAKGQANHFRVVQHVLQQFKQKFCYFLQQIENMDALFEAQFSPLADSGRALS 616

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK+IS+QPQR++CG CEEVYYLPQKGTIKLYKELTCPLDNFELLI S+PGPE
Sbjct: 617 KCGKCLRYMKYISAQPQRMFCGTCEEVYYLPQKGTIKLYKELTCPLDNFELLIFSMPGPE 676

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGIDTL+G++K   S+G   KG GMPCFLC HPTCRHS++ QGV
Sbjct: 677 GKSFPLCPYCYNSPPFEGIDTLFGSAKIG-STGKLGKGAGMPCFLCLHPTCRHSVVAQGV 736

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TT D+CA+CDSTIIEVDFN
Sbjct: 737 CACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRDRCAECDSTIIEVDFN 796

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRG---GR 360
           KKTTPLEDGATL  GCILCDE LHSL+E+KHG SF R   +RGRGRGRG GRGRG   GR
Sbjct: 797 KKTTPLEDGATLHTGCILCDELLHSLVEVKHGKSFGRGFGARGRGRGRGTGRGRGGYRGR 856

Query: 361 GRNSGKPMDPKMSFRDF 375
           GR +GK +DP+MSFRDF
Sbjct: 857 GRTAGKKVDPRMSFRDF 872

[Close]
BLAST of MELO3C006788 vs. NCBI nr
Match: gi|823245874|ref|XP_012455604.1| (PREDICTED: DNA topoisomerase 3-beta isoform X2 [Gossypium raimondii])

HSP 1 Score: 660.2 bits (1702), Expect = 2.1e-186
Identity = 311/377 (82.49%), Postives = 341/377 (90.45%), Query Frame = 1 

Query: 1   MEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCIDPDLCLPDIRS 60
           MEK+GIGTDASIPVHINNI ERNYVQVQAGRRLVPT LGITLIRGYQCIDPDLCLPDIRS
Sbjct: 454 MEKNGIGTDASIPVHINNICERNYVQVQAGRRLVPTTLGITLIRGYQCIDPDLCLPDIRS 513

Query: 61  FIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALFEAQFSPLADSGRALS 120
           FIEHQITLVAKGQA+H  VV+ VLQQFKQK+ YF+++I+ MDALFEAQFSPLADSGRALS
Sbjct: 514 FIEHQITLVAKGQANHFRVVQHVLQQFKQKFCYFLQQIENMDALFEAQFSPLADSGRALS 573

Query: 121 KCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFELLILSLPGPE 180
           KCGKCLRYMK+IS+QPQR++CG CEEVYYLPQKGTIKLYKELTCPLDNFELLI S+PGPE
Sbjct: 574 KCGKCLRYMKYISAQPQRMFCGTCEEVYYLPQKGTIKLYKELTCPLDNFELLIFSMPGPE 633

Query: 181 GKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFLCPHPTCRHSLITQGV 240
           GKSFPLCPYCYNSPPFEGIDTL+G++K   S+G   KG GMPCFLC HPTCRHS++ QGV
Sbjct: 634 GKSFPLCPYCYNSPPFEGIDTLFGSAKIG-STGKLGKGAGMPCFLCLHPTCRHSVVAQGV 693

Query: 241 CACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSDKCADCDSTIIEVDFN 300
           CACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TT D+CA+CDSTIIEVDFN
Sbjct: 694 CACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRDRCAECDSTIIEVDFN 753

Query: 301 KKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRGRGRGRGRGRG---GR 360
           KKTTPLEDGATL  GCILCDE LHSL+E+KHG SF R   +RGRGRGRG GRGRG   GR
Sbjct: 754 KKTTPLEDGATLHTGCILCDELLHSLVEVKHGKSFGRGFGARGRGRGRGTGRGRGGYRGR 813

Query: 361 GRNSGKPMDPKMSFRDF 375
           GR +GK +DP+MSFRDF
Sbjct: 814 GRTAGKKVDPRMSFRDF 829

The following BLAST results are available for this feature:
BLAST of MELO3C006788 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TOP3B_ARATH1.2e-17878.34DNA topoisomerase 3-beta OS=Arabidopsis thaliana GN=At2g32000 PE=2 SV=1[more]
TOP3B_ORYSJ1.1e-16975.40DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica GN=TOP3B PE=2 SV=1[more]
TOP3B_MOUSE2.4e-10551.88DNA topoisomerase 3-beta-1 OS=Mus musculus GN=Top3b PE=1 SV=1[more]
TOP3B_HUMAN2.4e-10551.61DNA topoisomerase 3-beta-1 OS=Homo sapiens GN=TOP3B PE=1 SV=1[more]
TOP3B_DROME4.1e-8947.08DNA topoisomerase 3-beta OS=Drosophila melanogaster GN=Top3beta PE=2 SV=2[more]
BLAST of MELO3C006788 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L9C7_CUCSA1.2e-21596.26DNA topoisomerase OS=Cucumis sativus GN=Csa_3G172970 PE=3 SV=1[more]
A0A0D2T8F3_GOSRA1.5e-18682.49DNA topoisomerase OS=Gossypium raimondii GN=B456_011G150100 PE=3 SV=1[more]
W9R2T8_9ROSA1.6e-18582.89DNA topoisomerase OS=Morus notabilis GN=L484_019920 PE=3 SV=1[more]
A0A061EL32_THECC2.2e-18281.55DNA topoisomerase OS=Theobroma cacao GN=TCM_020175 PE=3 SV=1[more]
A0A0K9RGT7_SPIOL4.9e-18280.48DNA topoisomerase OS=Spinacia oleracea GN=SOVF_068130 PE=3 SV=1[more]
BLAST of MELO3C006788 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G32000.17.0e-18078.34 DNA topoisomerase, type IA, core[more]
AT5G63920.18.7e-1333.83 topoisomerase 3alpha[more]
BLAST of MELO3C006788 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659077152|ref|XP_008439059.1|6.7e-225100.00PREDICTED: DNA topoisomerase 3-beta-1 [Cucumis melo][more]
gi|449459882|ref|XP_004147675.1|1.7e-21596.26PREDICTED: DNA topoisomerase 3-beta isoform X1 [Cucumis sativus][more]
gi|778679257|ref|XP_011651110.1|1.7e-21596.26PREDICTED: DNA topoisomerase 3-beta isoform X2 [Cucumis sativus][more]
gi|823245872|ref|XP_012455603.1|2.1e-18682.49PREDICTED: DNA topoisomerase 3-beta isoform X1 [Gossypium raimondii][more]
gi|823245874|ref|XP_012455604.1|2.1e-18682.49PREDICTED: DNA topoisomerase 3-beta isoform X2 [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000380Topo_IA
IPR013497Topo_IA_cen
IPR013824Topo_IA_cen_sub1
IPR023405Topo_IA_core_domain
Vocabulary: Molecular Function
TermDefinition
GO:0003677DNA binding
GO:0003917DNA topoisomerase type I activity
GO:0003916DNA topoisomerase activity
Vocabulary: Biological Process
TermDefinition
GO:0006265DNA topological change
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006265 DNA topological change
cellular_component GO:0005575 cellular_component
molecular_function GO:0003677 DNA binding
molecular_function GO:0003917 DNA topoisomerase type I activity
molecular_function GO:0003916 DNA topoisomerase activity
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
MU58498melon EST collection version 4.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C006788T1MELO3C006788T1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
MU58498MU58498transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000380DNA topoisomerase, type IAPANTHERPTHR11390PROKARYOTIC DNA TOPOISOMERASEcoord: 1..191
score: 6.8E-161coord: 220..331
score: 6.8E
IPR013497DNA topoisomerase, type IA, centralPFAMPF01131Topoisom_baccoord: 1..80
score: 2.1
IPR013824DNA topoisomerase, type IA, central region, subdomain 1GENE3DG3DSA:1.10.460.10coord: 241..247
score: 4.0E-25coord: 1..94
score: 4.0
IPR023405DNA topoisomerase, type IA, core domainunknownSSF56712Prokaryotic type I DNA topoisomerasecoord: 1..130
score: 2.75
NoneNo IPR availablePANTHERPTHR11390:SF20DNA TOPOISOMERASE 3-BETA-1coord: 1..191
score: 6.8E-161coord: 220..331
score: 6.8E