Cucsa.251840 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.251840 vs. Swiss-Prot
Match: TOP3B_ARATH (DNA topoisomerase 3-beta OS=Arabidopsis thaliana GN=At2g32000 PE=2 SV=1)

HSP 1 Score: 1395.2 bits (3610), Expect = 0.0e+00
Identity = 664/862 (77.03%), Postives = 751/862 (87.12%), Query Frame = 1

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GSTEVHEFDG F G  A+Y+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  QAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENICF 125
              +Q+W + DP DLF A +IK ESNPKAHIC+HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF    +++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYILQT 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI + 
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRMLL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL   V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTPPD 485
           + SI GE F+CTG RVT KGFT+IMPW AV+EKKLP FLKGE+IEV RV+LY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIE QITLVAKGQADHS VVQ V+QQFR+K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPT 725
           L+IYS+PGPEGKSFPLCPYCYNSPPFEGI+TL+GASK   +   +  G GMPC +CPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT ++C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF+R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRSSGKPMDPKMSFRDF 868
           GRGGR  S  K +DPKMSFRDF
Sbjct: 847 GRGGRRGS--KSVDPKMSFRDF 865

BLAST of Cucsa.251840 vs. Swiss-Prot
Match: TOP3B_ORYSJ (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica GN=TOP3B PE=2 SV=1)

HSP 1 Score: 1330.9 bits (3443), Expect = 0.0e+00
Identity = 635/868 (73.16%), Postives = 735/868 (84.68%), Query Frame = 1

Query: 2   VAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVF 61
           +APTVLMVAEKPSIALSIASALS G+MSTRKGST+VHEFDG F G HA++KVTSVIGHV 
Sbjct: 1   MAPTVLMVAEKPSIALSIASALSGGRMSTRKGSTDVHEFDGMFQGSHAFFKVTSVIGHVL 60

Query: 62  SIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGE 121
           S+DF   +Q+W+ TDP+DLF A V+++E NPKAHI +HL++EARGC  LVLWLDCDREGE
Sbjct: 61  SVDFPPAYQNWEGTDPMDLFVAPVLRSECNPKAHIRRHLAQEARGCTYLVLWLDCDREGE 120

Query: 122 NICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDL 181
           NIC+EVI+CTG   +   ++++RA+FSSVTEKDI+ A++NLV P+++EALAVDARQEIDL
Sbjct: 121 NICYEVIDCTGIPKSEVGRRIFRAKFSSVTEKDIMDAMNNLVLPSKDEALAVDARQEIDL 180

Query: 182 KVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPY 241
           KVGVAFTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+ QITTFKPEKFWS+  Y
Sbjct: 181 KVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQITTFKPEKFWSLKTY 240

Query: 242 ILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLL 301
           +++ G E+ L+W+R KLFD DVT MFQK V  DGI +VT+IS K+E K RP GLNTVNLL
Sbjct: 241 VIKDGNEIQLEWDRKKLFDFDVTVMFQKMVASDGILKVTDISVKEECKARPPGLNTVNLL 300

Query: 302 KVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYV 361
           KVASSALG GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF+  L  L  NP W + V
Sbjct: 301 KVASSALGIGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFRSALAALAHNPLWSNDV 360

Query: 362 RMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYI 421
           R LL  G+ KP+ G D GDHPPITPM LATE+ LG DAWRLYQY+CQHFIG++S DC+Y 
Sbjct: 361 RTLLDTGFVKPKQGHDAGDHPPITPMRLATEEALGTDAWRLYQYICQHFIGTVSPDCRYT 420

Query: 422 RTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNT 481
           RT I+F+  GE F+C G+RVT KGFTSIMPWLAV+E  +P + KG+ + + +VD+Y+G+T
Sbjct: 421 RTSIEFTSGGETFHCVGNRVTSKGFTSIMPWLAVSENNIPAYKKGDAVSIHKVDIYEGST 480

Query: 482 TPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGY 541
           TPPDYLSESELISLMEK+GIGTDASIPVH+NNI ERNYVQV +GRRLVPT LG TLIRGY
Sbjct: 481 TPPDYLSESELISLMEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGY 540

Query: 542 QCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFE 601
           QCID DLCLPDIR FIE QITL+AKG+ADH  VVQ VLQQF +KYSYFVKKI++MDALFE
Sbjct: 541 QCIDADLCLPDIRRFIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFE 600

Query: 602 AQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPL 661
           AQFSPLADSGR LSKCGKC RYMK+IS+QP RLYC  CEEVYYLPQ G+IKLYKE+ CPL
Sbjct: 601 AQFSPLADSGRLLSKCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPL 660

Query: 662 DNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMC 721
           D FELL++S+ GP+ KSFPLCP+CYNSPPFEGI+ L+GA K +  +G   KG GMPCF+C
Sbjct: 661 DGFELLLFSMVGPDAKSFPLCPFCYNSPPFEGIDKLFGALKLD-DTGKVGKGAGMPCFLC 720

Query: 722 PHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDK 781
            HPTC+ S+ITQGVCACPEC+GTL+LDPVSAP W+L CN+CNC+V LP  AH+ISTT  K
Sbjct: 721 LHPTCKQSMITQGVCACPECTGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKK 780

Query: 782 CADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGR 841
           C  C+STIIEVDFNKKTTPL+DGATL  GCILCDE LHSL+EMKHGKSF   RG RGRGR
Sbjct: 781 CPTCESTIIEVDFNKKTTPLKDGATLHEGCILCDELLHSLIEMKHGKSFFMRRG-RGRGR 840

Query: 842 GRGRGRG-GRG-RSSGKPMDPKMSFRDF 868
           GRGRGRG  RG R S +  DPKMSFRDF
Sbjct: 841 GRGRGRGSSRGRRGSSRHDDPKMSFRDF 866

BLAST of Cucsa.251840 vs. Swiss-Prot
Match: TOP3B_MOUSE (DNA topoisomerase 3-beta-1 OS=Mus musculus GN=Top3b PE=1 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 4.5e-264
Identity = 462/869 (53.16%), Postives = 586/869 (67.43%), Query Frame = 1

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKG---STEVHEFDGRFLGHHAYYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G MS+ KG   +  VH++ G F G   ++K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVM 62

Query: 65  SIDFQAGFQDWKSTDPLDLF-QASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 124
           ++DF   +  W   DP +LF QA   K E+NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEG 122

Query: 125 ENICFEVIECT----GFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDAR 184
           ENICFEV++          NG  K V+RARFSS+T+ DI  A+  L EP+ NEAL+VDAR
Sbjct: 123 ENICFEVLDAVLPVMNNAHNG-EKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDAR 182

Query: 185 QEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFW 244
           QE+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE +W
Sbjct: 183 QELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYW 242

Query: 245 SVCPYILQTGYE-LNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGL 304
            +   +     E L LDW+R ++FD ++  MF      +  A V   S K++ K RP  L
Sbjct: 243 VLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLAL 302

Query: 305 NTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNP 364
           NTV +L+VASSALG GPQ AMQ+AERLYTQG+ISYPRTE+T YP +FD KG+L     +P
Sbjct: 303 NTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHP 362

Query: 365 EWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLS 424
            W D V+ LL++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +HFI ++S
Sbjct: 363 YWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVS 422

Query: 425 SDCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVARV 484
            DCKY+++ I F I  E F C G  V   GFT IMPW +V  E+ LP   KG+   V  V
Sbjct: 423 HDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEV 482

Query: 485 DLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLG 544
            + +  T+PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLG
Sbjct: 483 KMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLG 542

Query: 545 ITLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKID 604
           I L+ GY  ID +L LP IRS +E Q+ L+A+G+AD+  V+   L  F++K+ YFV  I 
Sbjct: 543 IVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIA 602

Query: 605 HMDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLY 664
            MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ GTIKLY
Sbjct: 603 GMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLY 662

Query: 665 KELTCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGM 724
           KEL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 KGM
Sbjct: 663 KELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KKGM 722

Query: 725 GMPCFMCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAH 784
           G  C  C HPTC+HSL   G+  C EC +G LVLDP S P WK+ CN CN +    + AH
Sbjct: 723 G--CNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAH 782

Query: 785 RISTTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKS-FIR 844
           R+  + D C  C++ +++VDFNK  +PL    T   GCI CD     L+E+KH  S    
Sbjct: 783 RVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPM 842

Query: 845 NRGSRGRGRGRGRGRGGRGRSSGKPMDPK 862
           +RG  GR +GRGRGRG R      P  PK
Sbjct: 843 HRGGPGRRQGRGRGRGRRPPGKPNPRRPK 851

BLAST of Cucsa.251840 vs. Swiss-Prot
Match: TOP3B_HUMAN (DNA topoisomerase 3-beta-1 OS=Homo sapiens GN=TOP3B PE=1 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 2.2e-263
Identity = 456/868 (52.53%), Postives = 587/868 (67.63%), Query Frame = 1

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKG---STEVHEFDGRFLGHHAYYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G +S+ KG   +  VHE+ G F G    +K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVM 62

Query: 65  SIDFQAGFQDWKSTDPLDLF-QASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 124
           ++DF   +  W   DP +LF QA   K E+NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEG 122

Query: 125 ENICFEVIECT---GFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQ 184
           ENICFEV++         +G  K V+RARFSS+T+ DI  A+  L EP+ NEAL+VDARQ
Sbjct: 123 ENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQ 182

Query: 185 EIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWS 244
           E+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE +W 
Sbjct: 183 ELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWV 242

Query: 245 VCPYI-LQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLN 304
           +   +       L LDW+R ++FD ++  MF      +  A+V   S K++ K RP  LN
Sbjct: 243 LQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALN 302

Query: 305 TVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPE 364
           TV +L+VASS+LG GPQ AMQ AERLYTQG+ISYPRTE+T YP +FD KG+L     +P 
Sbjct: 303 TVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPY 362

Query: 365 WGDYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSS 424
           W D V+ LL++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +HFI ++S 
Sbjct: 363 WADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSH 422

Query: 425 DCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVARVD 484
           DCKY+++ I F I  E+F C+G  V   GFT +MPW +V  E+ LP   +G+   V  V 
Sbjct: 423 DCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVK 482

Query: 485 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 544
           + +  T PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLGI
Sbjct: 483 MLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGI 542

Query: 545 TLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDH 604
            L+ GY  ID +L LP IRS +E Q+ L+A+G+AD+  V+   L  F++K+ YFV  I  
Sbjct: 543 VLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAG 602

Query: 605 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYK 664
           MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ GTIKLYK
Sbjct: 603 MDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYK 662

Query: 665 ELTCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMG 724
           EL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 KGMG
Sbjct: 663 ELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KKGMG 722

Query: 725 MPCFMCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHR 784
             C  C HP+C+HSL   G+  C EC SG LVLDP S P WK+ CNKCN +    + AHR
Sbjct: 723 --CNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHR 782

Query: 785 ISTTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKS-FIRN 844
           +  + D C+ C++ +++VDFNK  +PL    T  +GC+ CD     L+E+KH  S    +
Sbjct: 783 VRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMH 842

Query: 845 RGSRGRGRGRGRGRGGRGRSSGKPMDPK 862
           RG  GR +GRGRGR  R      P  PK
Sbjct: 843 RGGPGRRQGRGRGRARRPPGKPNPRRPK 851

BLAST of Cucsa.251840 vs. Swiss-Prot
Match: TOP3B_DROME (DNA topoisomerase 3-beta OS=Drosophila melanogaster GN=Top3beta PE=2 SV=2)

HSP 1 Score: 817.4 bits (2110), Expect = 1.5e-235
Identity = 414/855 (48.42%), Postives = 558/855 (65.26%), Query Frame = 1

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGS---TEVHEFDGRFLGHHA-YYKVTSVIGHV 64
           +VLMVAEKPS+A S+A  LS+G+ + ++G+      HE+ G F    + ++++TSV GHV
Sbjct: 3   SVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHV 62

Query: 65  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 124
            S+DF   +  W   DP+ LF  +  K E+NPK ++ + L+ EARGCD LVLWLDCD+EG
Sbjct: 63  MSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEG 122

Query: 125 ENICFEVIECTGFGGNG--TRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQE 184
           ENICFEV++      N   + +  YRA FS++TEKDI KA++ L  PN NEA +VDARQE
Sbjct: 123 ENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQE 182

Query: 185 IDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSV 244
           +DL++G AFTRFQT FFQ +YG+LDS LISYGPCQTPTLGFCV+RH  I TFKPE FW +
Sbjct: 183 LDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL 242

Query: 245 CPYILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTV 304
              +L    E+ L+W RG++F  D+  M   RV E   A V  ++ K+  K +P  LNTV
Sbjct: 243 --QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTV 302

Query: 305 NLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWG 364
            L+++ SS LG GP  AMQ+AERLYTQG+ISYPRTE+  YP++FD    LH L  + ++G
Sbjct: 303 ELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFG 362

Query: 365 DYVRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDC 424
           +  R +L D    PR G D GDHPPITPM L       RD WR+Y+++C+HF+G++S D 
Sbjct: 363 EEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDL 422

Query: 425 KYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVN-EKKLPQFLKGEKIEVARVDLY 484
           KY  T  + S+  E F+CT S +   GFT +M W A   ++  P F++G ++ +  V L 
Sbjct: 423 KYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLI 482

Query: 485 QGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITL 544
           +  T PPDYL+ESELI+LME+HGIGTDASIPVHINNI +RNYV ++ GR+L+PT LGI L
Sbjct: 483 ESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVL 542

Query: 545 IRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMD 604
           + GYQ IDP+L LP +R+ +E  +TL+A+G A+   V++  ++ F+ K+ YFVK ID MD
Sbjct: 543 VHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMD 602

Query: 605 ALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKEL 664
           ALFE  FSPLA+SG+A S+CGKC RYMK+I ++P RL+C  C+E Y LP  G +K+Y+E 
Sbjct: 603 ALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALP-IGNVKVYREF 662

Query: 665 TCPLDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMP 724
            CPLD+F+LL +S  G +G+S+P CPYCYN PPF  +  L G                  
Sbjct: 663 KCPLDDFDLLAFS-TGVKGRSYPFCPYCYNHPPFSDMPHLGG------------------ 722

Query: 725 CFMCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRIS 784
           C  C +  C HSL T G+ +C EC +G LVLD   AP WKL CN+C+ ++   KGA +I+
Sbjct: 723 CNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKIT 782

Query: 785 TTRDKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS 844
               KC +C +  + V +    +  +DG+    GCI C      L+E KH     R   S
Sbjct: 783 VEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVE-KHRAVASRPVRS 833

Query: 845 RGRGRGRGRGRGGRG 852
            G  RG   GRGG G
Sbjct: 843 GGGFRGGKAGRGGGG 833

BLAST of Cucsa.251840 vs. TrEMBL
Match: A0A0A0L9C7_CUCSA (DNA topoisomerase OS=Cucumis sativus GN=Csa_3G172970 PE=3 SV=1)

HSP 1 Score: 1795.0 bits (4648), Expect = 0.0e+00
Identity = 867/867 (100.00%), Postives = 867/867 (100.00%), Query Frame = 1

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGGRGRSSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 867

BLAST of Cucsa.251840 vs. TrEMBL
Match: A0A0D2T8F3_GOSRA (DNA topoisomerase OS=Gossypium raimondii GN=B456_011G150100 PE=3 SV=1)

HSP 1 Score: 1477.6 bits (3824), Expect = 0.0e+00
Identity = 701/869 (80.67%), Postives = 785/869 (90.33%), Query Frame = 1

Query: 4   PTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSI 63
           P V+MVAEKPSIALSIAS LS GQMSTRKGSTEVHEF+G+FLG HA+YKVTSVIGHVFS+
Sbjct: 5   PKVIMVAEKPSIALSIASVLSRGQMSTRKGSTEVHEFNGKFLGFHAHYKVTSVIGHVFSV 64

Query: 64  DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI 123
           DF A +QDW +T+PLDLFQASV+KTE+NP+AHI +HLS+EARGC  LVLWLDCDREGENI
Sbjct: 65  DFPASYQDWNATNPLDLFQASVVKTEANPRAHISKHLSQEARGCGYLVLWLDCDREGENI 124

Query: 124 CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV 183
           CFEVIECT F  N  +K+VYRARFSSVTEKDILKA+DNLVEPNR+EALAVDARQEIDLKV
Sbjct: 125 CFEVIECTRFPMNEAKKRVYRARFSSVTEKDILKAMDNLVEPNRHEALAVDARQEIDLKV 184

Query: 184 GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL 243
           GVAFTRFQT++FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+LQI TFKPEKFW++ PYI+
Sbjct: 185 GVAFTRFQTSYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYLQINTFKPEKFWALRPYII 244

Query: 244 QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV 303
           Q GYEL L+W+R KLFD DV  MFQK V EDGI EV ++SEKQE K RPSGLNTVNLLKV
Sbjct: 245 QNGYELQLEWKRQKLFDIDVAMMFQKLVTEDGILEVIDVSEKQETKGRPSGLNTVNLLKV 304

Query: 304 ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM 363
           ASSALG+GPQ AMQ+AERLYTQGFISYPRTESTAYPSSFDFKGTL  L  NP W D V+ 
Sbjct: 305 ASSALGYGPQTAMQIAERLYTQGFISYPRTESTAYPSSFDFKGTLKVLENNPSWSDCVQR 364

Query: 364 LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT 423
           LL++GY KPR+GTD GDHPPITPM  ATED+LG DAWRLYQY+CQHF+G++S DCKY+RT
Sbjct: 365 LLAEGYHKPRTGTDAGDHPPITPMRPATEDVLGNDAWRLYQYICQHFMGTVSPDCKYVRT 424

Query: 424 KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP 483
           K++FSI GE F+C G RVTV+GFT IMPWLA+NEK LPQF KGEKIEV+RV+LY+G T+P
Sbjct: 425 KVEFSIGGEFFHCIGQRVTVQGFTYIMPWLAINEKNLPQFTKGEKIEVSRVELYEGETSP 484

Query: 484 PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC 543
           PDYLSESELISLMEK+GIGTDASIPVHINNI ERNYVQVQAGRRLVPT LGITLIRGYQC
Sbjct: 485 PDYLSESELISLMEKNGIGTDASIPVHINNICERNYVQVQAGRRLVPTTLGITLIRGYQC 544

Query: 544 IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ 603
           IDPDLCLPDIRSFIEHQITLVAKGQA+H  VVQ VLQQF+QK+ YF+++I++MDALFEAQ
Sbjct: 545 IDPDLCLPDIRSFIEHQITLVAKGQANHFRVVQHVLQQFKQKFCYFLQQIENMDALFEAQ 604

Query: 604 FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN 663
           FSPLADSGRALSKCGKCLRYMK+IS+QPQR++CG CEEVYYLPQKGTIKLYKELTCPLDN
Sbjct: 605 FSPLADSGRALSKCGKCLRYMKYISAQPQRMFCGTCEEVYYLPQKGTIKLYKELTCPLDN 664

Query: 664 FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH 723
           FELLI+S+PGPEGKSFPLCPYCYNSPPFEGI+TL+G++K   S+G   KG GMPCF+C H
Sbjct: 665 FELLIFSMPGPEGKSFPLCPYCYNSPPFEGIDTLFGSAKIG-STGKLGKGAGMPCFLCLH 724

Query: 724 PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA 783
           PTCRHS++ QGVCACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TTRD+CA
Sbjct: 725 PTCRHSVVAQGVCACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRDRCA 784

Query: 784 DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR 843
           +CDSTIIEVDFNKKTTPLEDGATL  GCILCDE LHSL+E+KHGKSF R  G+RGRGRGR
Sbjct: 785 ECDSTIIEVDFNKKTTPLEDGATLHTGCILCDELLHSLVEVKHGKSFGRGFGARGRGRGR 844

Query: 844 GRGRG-----GRGRSSGKPMDPKMSFRDF 868
           G GRG     GRGR++GK +DP+MSFRDF
Sbjct: 845 GTGRGRGGYRGRGRTAGKKVDPRMSFRDF 872

BLAST of Cucsa.251840 vs. TrEMBL
Match: A0A061EL32_THECC (DNA topoisomerase OS=Theobroma cacao GN=TCM_020175 PE=3 SV=1)

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 694/865 (80.23%), Postives = 777/865 (89.83%), Query Frame = 1

Query: 4   PTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSI 63
           P VLMVAEKPSIALSIAS LSHG+MSTR+GSTEVHEF+G FLG +A+YKVTSVIGHVFS+
Sbjct: 5   PKVLMVAEKPSIALSIASVLSHGKMSTRRGSTEVHEFNGTFLGFNAHYKVTSVIGHVFSV 64

Query: 64  DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI 123
           DF A +QDW  T+PLDLFQASVIKTESNPKAHI +HLS+EARGC  LVLWLDCDREGENI
Sbjct: 65  DFPASYQDWNVTNPLDLFQASVIKTESNPKAHISRHLSQEARGCGYLVLWLDCDREGENI 124

Query: 124 CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV 183
           CFEVIECTGF  N  +K+VYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKV
Sbjct: 125 CFEVIECTGFHMNEAKKRVYRARFSSVTEKDISKAMDNLVEPNRHEALAVDARQEIDLKV 184

Query: 184 GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL 243
           GVAFTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+LQI TFKPEKFW++ PYI+
Sbjct: 185 GVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYLQINTFKPEKFWALRPYII 244

Query: 244 QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV 303
           Q GY+L L+W+R KLFD DV  MFQK V EDGI EV ++SEKQE+K RPSGLNTVNLLKV
Sbjct: 245 QNGYDLQLEWKRQKLFDIDVAIMFQKLVTEDGILEVIDVSEKQESKGRPSGLNTVNLLKV 304

Query: 304 ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM 363
           ASSALGFGPQ AMQLAERLYTQGFISYPRTESTAYP+SFDFKGTL+ +  N  WGDYV+ 
Sbjct: 305 ASSALGFGPQTAMQLAERLYTQGFISYPRTESTAYPASFDFKGTLNVIANNSLWGDYVQR 364

Query: 364 LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT 423
           LL++GY KPRSGTD GDHPPITPM  ATED+LG+DAW LYQYVCQHFIG++S DCKY+R 
Sbjct: 365 LLAEGYHKPRSGTDAGDHPPITPMHSATEDILGKDAWSLYQYVCQHFIGTVSPDCKYVRR 424

Query: 424 KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP 483
           K++F + GE F+C G  VT +GFT IMPWLA+NEK LPQF KGEKIEV++V+LY+G T+P
Sbjct: 425 KVEFLVGGEYFHCIGQHVTAQGFTYIMPWLAINEKNLPQFTKGEKIEVSKVELYEGETSP 484

Query: 484 PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC 543
           PDYLSESELISLME++ IGTDASIPVHINNI ERNYVQVQ GR+LVPT LGITLIRGYQC
Sbjct: 485 PDYLSESELISLMERNRIGTDASIPVHINNICERNYVQVQGGRKLVPTALGITLIRGYQC 544

Query: 544 IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ 603
           IDPDLCLPDIRSFIEHQITLVAKGQA+HS VVQ VLQQF+QK+SYFV++I++MDALFEAQ
Sbjct: 545 IDPDLCLPDIRSFIEHQITLVAKGQANHSHVVQHVLQQFKQKFSYFVQQIENMDALFEAQ 604

Query: 604 FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN 663
           FSPLADSGRALSKCGKCLRYMK+IS+QP RL+CG CEEVY+LPQKGTIKLYKELTCPLDN
Sbjct: 605 FSPLADSGRALSKCGKCLRYMKYISTQPPRLFCGTCEEVYHLPQKGTIKLYKELTCPLDN 664

Query: 664 FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH 723
           FELLI+S+PGPEGKSFPLCPYCYNSPPFEG++TL+GA++   SSG   KG GMPCF+CPH
Sbjct: 665 FELLIFSMPGPEGKSFPLCPYCYNSPPFEGMDTLFGATRIG-SSGKLGKGAGMPCFLCPH 724

Query: 724 PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA 783
           PTCRHS++ QGVCACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TTR++CA
Sbjct: 725 PTCRHSVVAQGVCACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRERCA 784

Query: 784 DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR 843
           +CDSTIIEVDFNKKTTPLEDGATL  GCILCDE LHS +E+KHGKSF R  G RGRGRG 
Sbjct: 785 ECDSTIIEVDFNKKTTPLEDGATLHTGCILCDELLHSFVEVKHGKSFSRGLGGRGRGRGT 844

Query: 844 GR-GRGGRGRSSGKPMDPKMSFRDF 868
            R G  GRGR++GK +DPKMSFRDF
Sbjct: 845 RRGGYRGRGRAAGKQVDPKMSFRDF 868

BLAST of Cucsa.251840 vs. TrEMBL
Match: B9RRL8_RICCO (DNA topoisomerase OS=Ricinus communis GN=RCOM_1646110 PE=3 SV=1)

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 691/867 (79.70%), Postives = 770/867 (88.81%), Query Frame = 1

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           M  P VLMVAEKPSIALSIASALSHGQMS++KG TEVHEFDG FLG  A+YKVTSVIGH+
Sbjct: 1   MAPPVVLMVAEKPSIALSIASALSHGQMSSKKGGTEVHEFDGMFLGFQAHYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FS+DF   +QDW + DP+DLFQA ++K ESNPK HI +HLS+EARGC  LVLWLDCDREG
Sbjct: 61  FSVDFPQKYQDWTANDPMDLFQAPIVKAESNPKVHIRRHLSQEARGCGHLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGF  N +R++VYRARFSSV EKDILKA+DNLV+PNR+EALAVDARQEID
Sbjct: 121 ENICFEVIECTGFHLNDSRRRVYRARFSSVAEKDILKAMDNLVQPNRDEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQT+FFQGKYGNLDSR+ISYGPCQTPTLGFCVQR+LQI TFKPEKFWS+ P
Sbjct: 181 LKVGVAFTRFQTSFFQGKYGNLDSRVISYGPCQTPTLGFCVQRYLQIATFKPEKFWSLRP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           +++  GY+L L+WER +LFD DV  MFQK+V++DGI EV +IS+KQE K RPSGLNTVNL
Sbjct: 241 HLIIDGYQLQLEWERHRLFDLDVALMFQKQVLQDGILEVIDISQKQETKGRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFK  L TL+ N  WGD 
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKSILSTLLNNSIWGDS 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR LL +GY KPR GTD GDHPPITP+ LATEDMLG+DAWRLYQYVCQHFIGS+S DCKY
Sbjct: 361 VRRLLVEGYHKPRLGTDAGDHPPITPIRLATEDMLGKDAWRLYQYVCQHFIGSVSPDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           +RTK++FS+ GE F+C G  V V+GFTSIMPWLAV EK LPQ +KGEKIEV R++L +G 
Sbjct: 421 VRTKVEFSVGGEFFHCIGQHVIVEGFTSIMPWLAVTEKNLPQLVKGEKIEVLRMELLEGK 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYL+ESELISLMEK+GIGTDASIPVHINNI ERNYVQVQAGRRL PT LGITLIRG
Sbjct: 481 TTPPDYLTESELISLMEKNGIGTDASIPVHINNICERNYVQVQAGRRLAPTILGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHS VVQ VLQQF+QK++YFVK+ID+MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSRVVQHVLQQFKQKFTYFVKQIDNMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFS L DSGRALSKCGKCLRYMK+IS+QP RLYCG CEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSQLVDSGRALSKCGKCLRYMKYISTQPPRLYCGTCEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI+S+ GPEGK+FPLCPYCYN+PPFEG++TL+GASK N + G   KG GMPCF+
Sbjct: 661 LDNFELLIFSMAGPEGKTFPLCPYCYNNPPFEGVDTLFGASK-NGNFGKLGKGAGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLI QGVCACPECSGTLVLDPVSAP W+L CN CNCLV LP+GAHRI+TTR+
Sbjct: 721 CPHPTCRHSLIAQGVCACPECSGTLVLDPVSAPKWRLYCNMCNCLVLLPQGAHRIATTRE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           +C +CDSTIIEVDFNK TTPLEDGATL +GCILCD+ LHSL+E+KHGKSF +  G+  RG
Sbjct: 781 RCPECDSTIIEVDFNKNTTPLEDGATLHIGCILCDDLLHSLVEVKHGKSFFKRLGAGRRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RG GRGR GRGRSSG+  DPKMSFRDF
Sbjct: 841 RGAGRGR-GRGRSSGRRGDPKMSFRDF 865

BLAST of Cucsa.251840 vs. TrEMBL
Match: A0A067KC20_JATCU (DNA topoisomerase OS=Jatropha curcas GN=JCGZ_13118 PE=3 SV=1)

HSP 1 Score: 1441.0 bits (3729), Expect = 0.0e+00
Identity = 683/867 (78.78%), Postives = 766/867 (88.35%), Query Frame = 1

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           M  P VLMVAEKPSIALSIAS LS G+MST+K STEVHEFDG+FLG HA+YKVTSVIGH+
Sbjct: 1   MAPPVVLMVAEKPSIALSIASVLSCGRMSTKKVSTEVHEFDGKFLGFHAHYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FS+DF   +QDW   DP+DLFQA ++K+E+NPKAHI +HLS+EARGC  LVLWLDCDREG
Sbjct: 61  FSVDFPPKYQDWTVIDPMDLFQAQIVKSEANPKAHIRRHLSQEARGCGHLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGF  N  R++VYRARFSSV EKDILKA+ NL++PNR+EALAVDARQEID
Sbjct: 121 ENICFEVIECTGFHINDARRRVYRARFSSVAEKDILKAMGNLIQPNRDEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQT+FFQGKYGNLDSR+ISYGPCQTPTLGFCVQR+LQI TF+PEKFW++ P
Sbjct: 181 LKVGVAFTRFQTSFFQGKYGNLDSRVISYGPCQTPTLGFCVQRYLQIATFRPEKFWALQP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           +I+  GYEL L+WER +LFD D+  MFQK VI+DGI EV ++S+KQE+K RPSGLNTVNL
Sbjct: 241 HIIIDGYELQLEWERHRLFDLDIAMMFQKLVIQDGILEVIDVSQKQESKGRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTL  L  N  WG Y
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLGMLANNSMWGSY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           V+ L+ +GY KPR GTD GDHPPITP+ LATEDMLG+DAW +Y YVCQHF+GS+S DCKY
Sbjct: 361 VQRLIVEGYHKPRLGTDAGDHPPITPIRLATEDMLGKDAWSIYHYVCQHFLGSVSPDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           +RTK++FS+AGE F+C G  VTV+GFTSIMPWL V+EK LPQF KGEK+EV++VDL++G 
Sbjct: 421 VRTKVEFSVAGEFFHCIGQHVTVEGFTSIMPWLVVSEKNLPQFKKGEKVEVSKVDLFEGK 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T PPDYLSESELISLME++GIGTDASIP HINNI ERNYVQVQAGRRLVPT LGITLIRG
Sbjct: 481 TVPPDYLSESELISLMERNGIGTDASIPTHINNICERNYVQVQAGRRLVPTTLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITL+AKGQADHS VVQ VLQQF+QKYSYFVK+ID+MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITLIAKGQADHSRVVQHVLQQFKQKYSYFVKRIDNMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFS L DSGRALSKCGKCLRYMK+IS+QPQRLYCG CEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSQLVDSGRALSKCGKCLRYMKYISTQPQRLYCGTCEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI+S+ GPEGK+FPLCPYCYN+PPFEGI TL+  SK N + G   KG GMPCF+
Sbjct: 661 LDNFELLIFSMAGPEGKTFPLCPYCYNNPPFEGIETLFSTSK-NGNFGKLGKGAGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLI QGVCACPECSGTLVLDPVSAP W+L CN CNCLV LP GAHRI TTR+
Sbjct: 721 CPHPTCRHSLIAQGVCACPECSGTLVLDPVSAPKWRLYCNMCNCLVLLPLGAHRIGTTRE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           +C +CDS I+EVDFNK TTPLEDGATL +GCILCD+ LHSL+E+KHGKSF +  G+RGRG
Sbjct: 781 RCPECDSMILEVDFNKNTTPLEDGATLHIGCILCDDLLHSLVEVKHGKSFFKRMGARGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRG  R GRGRSSG+  DPKMSFRDF
Sbjct: 841 RGRGSYR-GRGRSSGRRGDPKMSFRDF 865

BLAST of Cucsa.251840 vs. TAIR10
Match: AT2G32000.1 (AT2G32000.1 DNA topoisomerase, type IA, core)

HSP 1 Score: 1395.2 bits (3610), Expect = 0.0e+00
Identity = 664/862 (77.03%), Postives = 751/862 (87.12%), Query Frame = 1

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GSTEVHEFDG F G  A+Y+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  QAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENICF 125
              +Q+W + DP DLF A +IK ESNPKAHIC+HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF    +++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYILQT 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI + 
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRMLL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL   V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTPPD 485
           + SI GE F+CTG RVT KGFT+IMPW AV+EKKLP FLKGE+IEV RV+LY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIE QITLVAKGQADHS VVQ V+QQFR+K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+CG CEEVYYLPQKGT+KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPHPT 725
           L+IYS+PGPEGKSFPLCPYCYNSPPFEGI+TL+GASK   +   +  G GMPC +CPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT ++C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF+R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRSSGKPMDPKMSFRDF 868
           GRGGR  S  K +DPKMSFRDF
Sbjct: 847 GRGGRRGS--KSVDPKMSFRDF 865

BLAST of Cucsa.251840 vs. TAIR10
Match: AT5G63920.1 (AT5G63920.1 topoisomerase 3alpha)

HSP 1 Score: 387.9 bits (995), Expect = 1.7e-107
Identity = 236/640 (36.88%), Postives = 349/640 (54.53%), Query Frame = 1

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGST---EVHEFDGRFLGHHAYYKVTSVIGHVF 64
           TVL VAEKPS+A S+A  LS G   TR+G +   ++ EFD    G      +TSVIGH+ 
Sbjct: 10  TVLNVAEKPSVAKSVAGILSRGTFRTREGRSRYNKIFEFDYAINGQPCRMLMTSVIGHLM 69

Query: 65  SIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGE 124
            ++F   ++ W S DP DL+QA V+K     K  I + L  EAR  D LVLWLDCDREGE
Sbjct: 70  ELEFADRYRKWHSCDPADLYQAPVMKHVPEDKKDIKKTLEEEARKSDWLVLWLDCDREGE 129

Query: 125 NICFEVIE-CTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 184
           NI FEV++ C     N     + RA FS++ ++DI +A+ NL +PN+  A AVDARQEID
Sbjct: 130 NIAFEVVDVCRAVKHN---LFIRRAHFSALIDRDIHEAVQNLRDPNQLFAEAVDARQEID 189

Query: 185 LKVGVAFTRFQTTFFQGKY-----GNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 244
           L++G +FTRFQT   + ++     G   SR+ISYGPCQ PTLGF V+R+ +I   +PE+F
Sbjct: 190 LRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEF 249

Query: 245 WSV-CPYILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQEN-KVRPS 304
           W++ C +  + G     +W RG LFD     +  +  +E+  A V  +   +E  K  P 
Sbjct: 250 WTINCSHQSEEGLA-TFNWMRGHLFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPY 309

Query: 305 GLNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVK 364
            LNT+ L K AS       +  M++AE LY  GFISYPRTE+ ++ S  D +  +    +
Sbjct: 310 PLNTIELEKRASRYFRLSSEHTMKVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTR 369

Query: 365 NPEWGDYVRMLLSDGYSKPRS----GTDVGDHPPITPMCLAT-EDMLGRDAWRLYQYVCQ 424
           +P WG Y + LL       R+    G D   HPPI P   ++ E    RD   +Y+ V +
Sbjct: 370 HPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVR 429

Query: 425 HFIGSLSSDCKYIRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEK 484
           H++  +S       T ++  IAGE F+ +G  +  K +  +  + +     +P + KG++
Sbjct: 430 HYLACVSQPAVAAETTVEIDIAGERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQ 489

Query: 485 IEVARVDLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL 544
                + L    T PP  L E++L+S M+K GIGTDA++  HI  + +R Y    A  R 
Sbjct: 490 FIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRF 549

Query: 545 VPTNLGITLIRGYQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKY-S 604
            PTNLG  L+ GY  +  +L  P++R+ +EH +  V+ G+   + V+++ LQQ +  +  
Sbjct: 550 SPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLD 609

Query: 605 YFVKK---IDHMDALFE----AQFSPLADSGRALSKCGKC 621
             VKK   ++ M   FE       S    +G  + +C  C
Sbjct: 610 ARVKKSKLLEAMTIFFERSNNTDESESQTAGEVVRRCNLC 645

BLAST of Cucsa.251840 vs. TAIR10
Match: AT4G31210.1 (AT4G31210.1 DNA topoisomerase, type IA, core)

HSP 1 Score: 70.9 bits (172), Expect = 4.5e-12
Identity = 111/523 (21.22%), Postives = 200/523 (38.24%), Query Frame = 1

Query: 51  YKVTSVIGHVFSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCL 110
           Y+V    GH+  +  ++G     S  P D F + V +  S+   HI + +     G + L
Sbjct: 496 YEVLPSYGHIRDLATRSG-----SVRPDDDF-SMVWEVPSSAWTHI-KSIKVALNGAENL 555

Query: 111 VLWLDCDREGENICFEVIECTGF-GGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNE 170
           +L  D DREGE I + +IE     G       V R  F  +TE  I  AL +  E + + 
Sbjct: 556 ILASDPDREGEAIAWHIIEMLQQQGALHESMTVARVVFHEITESAIKSALQSPREIDGDL 615

Query: 171 ALAVDARQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITT 230
             A  AR+ +D  +G   +        G          S G  Q+  L     R  +I  
Sbjct: 616 VHAYLARRALDYLIGFNISPLLWRKLPG--------CPSAGRVQSAALALVCDRESEIDG 675

Query: 231 FKPEKFWSVCPYILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQEN- 290
           FKP+++W+V       G ++    +    F   +T++  KR+ +  I+      + ++  
Sbjct: 676 FKPQEYWTV-------GIKVK-GKDNSATFSAHLTSLNSKRLNQLSISSEANAQDIEQRI 735

Query: 291 ----------------KVRPSGLNTVNLLKVASSALGFGPQMAMQLAERLY--------- 350
                           K  P+   T  L + A++ L F     M+LA++LY         
Sbjct: 736 KSEGFLVKGTKTSTTRKNPPTPYITSTLQQDAANKLHFSTAHTMKLAQKLYEGVQLSDGK 795

Query: 351 TQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRMLLSDGYSKPRSGTDVGDHPP 410
           + G I+Y RT+        +    + +LV      ++        + K ++  +   H  
Sbjct: 796 SAGLITYMRTDGLHIAD--EAIKDIQSLVAERYGKNFTSDSPRKYFKKVKNAQEA--HEA 855

Query: 411 ITPMCL-----ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKIQFSIAGE--IFNC 470
           I P  +         +L  D+ +LY  +    +          + ++    A E  IF  
Sbjct: 856 IRPTDIRRLPSTIASLLDADSLKLYTLIWSRAVACQMEPASIAQIQLDIGNASESIIFRS 915

Query: 471 TGSRVTVKGFTSIMP-----WLAVNE-----------KKLPQFLKGEKIEVARVDLYQGN 524
           + S+V   G+ ++        +  N+           K L     G+ +++  V+L Q +
Sbjct: 916 SCSKVEFLGYQAVYEDPEAKAIKNNDNDQSSEREETFKTLSSLKDGDLLQIGEVELKQHH 975

BLAST of Cucsa.251840 vs. NCBI nr
Match: gi|449459882|ref|XP_004147675.1| (PREDICTED: DNA topoisomerase 3-beta isoform X1 [Cucumis sativus])

HSP 1 Score: 1795.0 bits (4648), Expect = 0.0e+00
Identity = 867/867 (100.00%), Postives = 867/867 (100.00%), Query Frame = 1

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGGRGRSSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGGRGRSSGKPMDPKMSFRDF 867

BLAST of Cucsa.251840 vs. NCBI nr
Match: gi|659077152|ref|XP_008439059.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Cucumis melo])

HSP 1 Score: 1750.3 bits (4532), Expect = 0.0e+00
Identity = 847/869 (97.47%), Postives = 857/869 (98.62%), Query Frame = 1

Query: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120
           FSIDFQAGFQDWK+T+PLDLFQASVIKTESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVV+SVLQQF+QKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGASK NPSSGTSSKGMGMPCF+
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT D
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGS--RG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SFIRNR S  RG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRGGRGRSSGKPMDPKMSFRDF 868
           RGRGRGRGRGGRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of Cucsa.251840 vs. NCBI nr
Match: gi|778679257|ref|XP_011651110.1| (PREDICTED: DNA topoisomerase 3-beta isoform X2 [Cucumis sativus])

HSP 1 Score: 1672.5 bits (4330), Expect = 0.0e+00
Identity = 804/804 (100.00%), Postives = 804/804 (100.00%), Query Frame = 1

Query: 64  DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI 123
           DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI
Sbjct: 4   DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI 63

Query: 124 CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV 183
           CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV
Sbjct: 64  CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV 123

Query: 184 GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL 243
           GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL
Sbjct: 124 GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL 183

Query: 244 QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV 303
           QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV
Sbjct: 184 QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV 243

Query: 304 ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM 363
           ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM
Sbjct: 244 ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM 303

Query: 364 LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT 423
           LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT
Sbjct: 304 LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT 363

Query: 424 KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP 483
           KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP
Sbjct: 364 KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP 423

Query: 484 PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC 543
           PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC
Sbjct: 424 PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC 483

Query: 544 IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ 603
           IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ
Sbjct: 484 IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ 543

Query: 604 FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN 663
           FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN
Sbjct: 544 FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN 603

Query: 664 FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH 723
           FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH
Sbjct: 604 FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH 663

Query: 724 PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA 783
           PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA
Sbjct: 664 PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA 723

Query: 784 DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR 843
           DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR
Sbjct: 724 DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR 783

Query: 844 GRGRGGRGRSSGKPMDPKMSFRDF 868
           GRGRGGRGRSSGKPMDPKMSFRDF
Sbjct: 784 GRGRGGRGRSSGKPMDPKMSFRDF 807

BLAST of Cucsa.251840 vs. NCBI nr
Match: gi|823245872|ref|XP_012455603.1| (PREDICTED: DNA topoisomerase 3-beta isoform X1 [Gossypium raimondii])

HSP 1 Score: 1477.6 bits (3824), Expect = 0.0e+00
Identity = 701/869 (80.67%), Postives = 785/869 (90.33%), Query Frame = 1

Query: 4   PTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSI 63
           P V+MVAEKPSIALSIAS LS GQMSTRKGSTEVHEF+G+FLG HA+YKVTSVIGHVFS+
Sbjct: 5   PKVIMVAEKPSIALSIASVLSRGQMSTRKGSTEVHEFNGKFLGFHAHYKVTSVIGHVFSV 64

Query: 64  DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI 123
           DF A +QDW +T+PLDLFQASV+KTE+NP+AHI +HLS+EARGC  LVLWLDCDREGENI
Sbjct: 65  DFPASYQDWNATNPLDLFQASVVKTEANPRAHISKHLSQEARGCGYLVLWLDCDREGENI 124

Query: 124 CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV 183
           CFEVIECT F  N  +K+VYRARFSSVTEKDILKA+DNLVEPNR+EALAVDARQEIDLKV
Sbjct: 125 CFEVIECTRFPMNEAKKRVYRARFSSVTEKDILKAMDNLVEPNRHEALAVDARQEIDLKV 184

Query: 184 GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL 243
           GVAFTRFQT++FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+LQI TFKPEKFW++ PYI+
Sbjct: 185 GVAFTRFQTSYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYLQINTFKPEKFWALRPYII 244

Query: 244 QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV 303
           Q GYEL L+W+R KLFD DV  MFQK V EDGI EV ++SEKQE K RPSGLNTVNLLKV
Sbjct: 245 QNGYELQLEWKRQKLFDIDVAMMFQKLVTEDGILEVIDVSEKQETKGRPSGLNTVNLLKV 304

Query: 304 ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM 363
           ASSALG+GPQ AMQ+AERLYTQGFISYPRTESTAYPSSFDFKGTL  L  NP W D V+ 
Sbjct: 305 ASSALGYGPQTAMQIAERLYTQGFISYPRTESTAYPSSFDFKGTLKVLENNPSWSDCVQR 364

Query: 364 LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT 423
           LL++GY KPR+GTD GDHPPITPM  ATED+LG DAWRLYQY+CQHF+G++S DCKY+RT
Sbjct: 365 LLAEGYHKPRTGTDAGDHPPITPMRPATEDVLGNDAWRLYQYICQHFMGTVSPDCKYVRT 424

Query: 424 KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP 483
           K++FSI GE F+C G RVTV+GFT IMPWLA+NEK LPQF KGEKIEV+RV+LY+G T+P
Sbjct: 425 KVEFSIGGEFFHCIGQRVTVQGFTYIMPWLAINEKNLPQFTKGEKIEVSRVELYEGETSP 484

Query: 484 PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC 543
           PDYLSESELISLMEK+GIGTDASIPVHINNI ERNYVQVQAGRRLVPT LGITLIRGYQC
Sbjct: 485 PDYLSESELISLMEKNGIGTDASIPVHINNICERNYVQVQAGRRLVPTTLGITLIRGYQC 544

Query: 544 IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ 603
           IDPDLCLPDIRSFIEHQITLVAKGQA+H  VVQ VLQQF+QK+ YF+++I++MDALFEAQ
Sbjct: 545 IDPDLCLPDIRSFIEHQITLVAKGQANHFRVVQHVLQQFKQKFCYFLQQIENMDALFEAQ 604

Query: 604 FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN 663
           FSPLADSGRALSKCGKCLRYMK+IS+QPQR++CG CEEVYYLPQKGTIKLYKELTCPLDN
Sbjct: 605 FSPLADSGRALSKCGKCLRYMKYISAQPQRMFCGTCEEVYYLPQKGTIKLYKELTCPLDN 664

Query: 664 FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH 723
           FELLI+S+PGPEGKSFPLCPYCYNSPPFEGI+TL+G++K   S+G   KG GMPCF+C H
Sbjct: 665 FELLIFSMPGPEGKSFPLCPYCYNSPPFEGIDTLFGSAKIG-STGKLGKGAGMPCFLCLH 724

Query: 724 PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA 783
           PTCRHS++ QGVCACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TTRD+CA
Sbjct: 725 PTCRHSVVAQGVCACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRDRCA 784

Query: 784 DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR 843
           +CDSTIIEVDFNKKTTPLEDGATL  GCILCDE LHSL+E+KHGKSF R  G+RGRGRGR
Sbjct: 785 ECDSTIIEVDFNKKTTPLEDGATLHTGCILCDELLHSLVEVKHGKSFGRGFGARGRGRGR 844

Query: 844 GRGRG-----GRGRSSGKPMDPKMSFRDF 868
           G GRG     GRGR++GK +DP+MSFRDF
Sbjct: 845 GTGRGRGGYRGRGRTAGKKVDPRMSFRDF 872

BLAST of Cucsa.251840 vs. NCBI nr
Match: gi|590656024|ref|XP_007034151.1| (DNA topoisomerase, type IA, core [Theobroma cacao])

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 694/865 (80.23%), Postives = 777/865 (89.83%), Query Frame = 1

Query: 4   PTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHVFSI 63
           P VLMVAEKPSIALSIAS LSHG+MSTR+GSTEVHEF+G FLG +A+YKVTSVIGHVFS+
Sbjct: 5   PKVLMVAEKPSIALSIASVLSHGKMSTRRGSTEVHEFNGTFLGFNAHYKVTSVIGHVFSV 64

Query: 64  DFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREGENI 123
           DF A +QDW  T+PLDLFQASVIKTESNPKAHI +HLS+EARGC  LVLWLDCDREGENI
Sbjct: 65  DFPASYQDWNVTNPLDLFQASVIKTESNPKAHISRHLSQEARGCGYLVLWLDCDREGENI 124

Query: 124 CFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKV 183
           CFEVIECTGF  N  +K+VYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKV
Sbjct: 125 CFEVIECTGFHMNEAKKRVYRARFSSVTEKDISKAMDNLVEPNRHEALAVDARQEIDLKV 184

Query: 184 GVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIL 243
           GVAFTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+LQI TFKPEKFW++ PYI+
Sbjct: 185 GVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYLQINTFKPEKFWALRPYII 244

Query: 244 QTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNLLKV 303
           Q GY+L L+W+R KLFD DV  MFQK V EDGI EV ++SEKQE+K RPSGLNTVNLLKV
Sbjct: 245 QNGYDLQLEWKRQKLFDIDVAIMFQKLVTEDGILEVIDVSEKQESKGRPSGLNTVNLLKV 304

Query: 304 ASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDYVRM 363
           ASSALGFGPQ AMQLAERLYTQGFISYPRTESTAYP+SFDFKGTL+ +  N  WGDYV+ 
Sbjct: 305 ASSALGFGPQTAMQLAERLYTQGFISYPRTESTAYPASFDFKGTLNVIANNSLWGDYVQR 364

Query: 364 LLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRT 423
           LL++GY KPRSGTD GDHPPITPM  ATED+LG+DAW LYQYVCQHFIG++S DCKY+R 
Sbjct: 365 LLAEGYHKPRSGTDAGDHPPITPMHSATEDILGKDAWSLYQYVCQHFIGTVSPDCKYVRR 424

Query: 424 KIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTP 483
           K++F + GE F+C G  VT +GFT IMPWLA+NEK LPQF KGEKIEV++V+LY+G T+P
Sbjct: 425 KVEFLVGGEYFHCIGQHVTAQGFTYIMPWLAINEKNLPQFTKGEKIEVSKVELYEGETSP 484

Query: 484 PDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQC 543
           PDYLSESELISLME++ IGTDASIPVHINNI ERNYVQVQ GR+LVPT LGITLIRGYQC
Sbjct: 485 PDYLSESELISLMERNRIGTDASIPVHINNICERNYVQVQGGRKLVPTALGITLIRGYQC 544

Query: 544 IDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALFEAQ 603
           IDPDLCLPDIRSFIEHQITLVAKGQA+HS VVQ VLQQF+QK+SYFV++I++MDALFEAQ
Sbjct: 545 IDPDLCLPDIRSFIEHQITLVAKGQANHSHVVQHVLQQFKQKFSYFVQQIENMDALFEAQ 604

Query: 604 FSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCPLDN 663
           FSPLADSGRALSKCGKCLRYMK+IS+QP RL+CG CEEVY+LPQKGTIKLYKELTCPLDN
Sbjct: 605 FSPLADSGRALSKCGKCLRYMKYISTQPPRLFCGTCEEVYHLPQKGTIKLYKELTCPLDN 664

Query: 664 FELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFMCPH 723
           FELLI+S+PGPEGKSFPLCPYCYNSPPFEG++TL+GA++   SSG   KG GMPCF+CPH
Sbjct: 665 FELLIFSMPGPEGKSFPLCPYCYNSPPFEGMDTLFGATRIG-SSGKLGKGAGMPCFLCPH 724

Query: 724 PTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRDKCA 783
           PTCRHS++ QGVCACPECSGTLVLDPVSAP W+L CNKCNCLV LP+GAHRI+TTR++CA
Sbjct: 725 PTCRHSVVAQGVCACPECSGTLVLDPVSAPKWRLYCNKCNCLVLLPEGAHRIATTRERCA 784

Query: 784 DCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRGRGR 843
           +CDSTIIEVDFNKKTTPLEDGATL  GCILCDE LHS +E+KHGKSF R  G RGRGRG 
Sbjct: 785 ECDSTIIEVDFNKKTTPLEDGATLHTGCILCDELLHSFVEVKHGKSFSRGLGGRGRGRGT 844

Query: 844 GR-GRGGRGRSSGKPMDPKMSFRDF 868
            R G  GRGR++GK +DPKMSFRDF
Sbjct: 845 RRGGYRGRGRAAGKQVDPKMSFRDF 868