MELO3C004787.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C004787.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
Descriptioncytoplasmic tRNA 2-thiolation protein 1
Locationchr12 : 10400114 .. 10404538 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACCTTTCCCCGAAGGGTGTCCAACAGCTGCTTTTGGAAGTTGGCAATAGATCCTCGACCACCCTACTTCAGTTGAAGAACTTCTATCTCTTCTCGACGTTAGCCCTTCTGTTTTCCTTCTCTACGTTTTTCAGCAGCTTGTTTTTGTTTAGGGCTTTTCCGACGGACTGTGTTTTACTGAATTAGCTTTTCAGCCTGGTAGAATGCTGTCTTTGAATTTTCTTTTGCTCTACGTTATACTCTGATATTTTGATATATCTGAAGCTTTAGATTTCAAGTAGCACAACTTAAATTAGAACAATATTTGGTTTACATTTATTCAAGTTTACTGGAATTATTCTTTTGGGTAGGGTTACATATGTCTGAAAACTGTTAAGGAGGCAATTGATTGGGATTAAGTGAATGTTTGAAAACTGGTAAGGAGTGGATTGCTTCCCGGAATGTTGATAATGTGTCTTCGTGTAATGGGGGTTCGTGCTTTTTAGATCAGTCAGGCAGTACAATATCACTTAACATTTGTTACTGTGACTTTTAAATCTGTCTTTCACCTTCATTCCTTAAACTCCACATTGAATGTATAGTGAGGTATTTCCCCACCTCATTGCTTTTCTTATCCTGCTAATGTAGTTAGTTATTCTCCCTCTTCTCTTCCCTCATTCTGTTGAATTAAAATATAATGGAATACTAGCAGCCAAATGGGCCTTCGTGGTCTTTGGTATTTGTTTATTTGTTTGTTTGTCTTTATCTATATGGGATCTTAATTTTTGTGGTTATGACAACTGACAAGCGCTTTGCATGTTTCTTTGACTTCACAATAATTTATAATCGGCTTGATTGCAGAAAATTGAGCGTTTGCTGGCAAAAGTTAAACAATCCCCTTCAAAATCAATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGGTAATCAGAAATTTGCTCGATTGAATGCCAATTTCCCACTTGTCGAATCTACTTCTAAAGAGTTGAATGTATGCTGCTGTTCAGGAGGTATTTCATCTTATAGTATCATCCTTTGAAAATCTCTCTGACAAGTCAAGTCGATCATATGTAAAATGCGCATCCATTCTTGAAACTGTTCCGAGGGTCAGATTATATGTGGCCATGCTGGATTTGGAATGTGATGCATTGATATGTTCCAACATTTCCTTAAGACAGTAAGGTATTATACTAGATTTTGTTTTGAAATAAAATTCCTTTTTAAATTTTTACTTATAAGACTATTTGTGGTAGCTATTAATTTTCCAACAATTCAAGTTCTTTTGTGAACAAATATAATTTGTTTCTCCATGAAATACTACTAGACTTCTGTTTTGAATCAGAACTTGACTTACTCCAGTATGACCATTATATTGGGTGTTTTGAATGAGATGTAGCAGTGGTATTTGAGTACATTTGAATGATTGTGTTAGACCCCCCAATATTACACACATATAAGAGAAGGGGCAGAAACGGGAATTAACATGGCTTATGCCAATTCGGGGAGCAAAGAGAGAGAAAGCATCTCCTGAGGGCCCACTGGGGAGTTGGTATTTATATAAAATGTCTTTGGGGATTGCTGTCATTAGGTTTTATGTTGTCTTTTTAGGTGAAGGCCGCCATAGCCTTCATTTGGGAGAAGTTTCCAGCCTTCTCTAAGGAGCTGGGTGTTAGTATTGTTTTTACTATTATTTTCTGGATTATAGCTCTGTTTTGTGGAACATTTTGAGTGTTATGATCAATATATTATAACAGAGTGCTGCCTCTGTTTTGTCTTGTTGGGACTTTTCTGCTTTTCAGGTTAAGAATCTTAACAAATTATCAAGAAAAACTCGGTTAAAAATATAATGCAGTGAATTAGTTGACTGTATGCATTGTAATAAGTTTGGCTTTGAACTATTGTCTGTTAAATAGGTTTTATTGGTTGAATGTGAATCCTTGTTACACTACAAGAAATTCTGGATCTCCTGACGTTGGTTTACACCGTCGGGAGATAAGGCATCTTCTGACGCAACAATTAATGAGTTGGGAGATGCAATATCCCCTGATGCCTTCATGCACGGCCTCGGAGATAGAAGAGAACTCCCGACGCCGTTAGTAATGCATCGGAGAACTGCTGACACTTTAATTAAAACGTTGACAGTTTTGGGATAACTCTCGACACTGTGTCGGGCTTTGTCGGAAGTTGGTCTTTAAAAACCCTCCTAGATTTGTATTTCACAGATCAGTGAAGAAGGGGAAGGGGAAATGAGAGACTCAGCTGAAAACCACCACCGTCGCTCGTGGCATCGCCCCGCCGCCGTTCTCGTCGTTTGCCCCGTCTCTTCTGGTGCCGCTGCTGTCCTCCTGTGTCTTTCTTGCTGCTGCTGCCCCTTTCTCTTCCGATATGTAACTTATTTAAGTTAGATTTAAATTTTGCCCTAAAATCCTAATTTGTTCAAAAACAATTGATTTCTAAAGGATTATGAGAGATGGTTTTTCAAAATCTATTGATTTCATAAGAGAGTAAAAAAGTGGTTATTAGCCATAATCTATTGATAGCCTTCTTCACTGTTTTTTGTTAATTCTCCCTTTGAGTTTTATTGGCCGTCTCTTATCCAAAGAATATTCTTCTCTATTAAGGCTTGTGGAAGCTTCCCTATCATTGTTTATCTCCTTCATTTTGTTCTGTTTGATTGGTGGTTATTGAAGGGACTCAATATCTTTTCTCTGAATGCTGATTTTTCAAGGTTTCAATGTATTTTCAAGACAAGCGTTGGCCTTAGCTTATTTGATTTGAAGTTTCCAGAGATAAAGCTTCTTTTTGTTGGTGTCTCTCTCCATTCTATTTGCAGAATTTTGTGACTTATGCTTAACCAGGATTGTATCTATTCTAGTTTAGGTATCTTGTATTGATCTATGTATTACTATAAGCATACTTGGCAAGTTCTCTTTTTGGTTCTTTGCGTATTAAGCATGGTTTCATTGTATTGATGGAAAATTTTCTCCTGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGGTATTTTAATAGTAAGACTTTTTTTCTGTCAATTACTTTTGTCCCTCATTTGGGCTGTATTAGATTGAAGTGATGCTTTGAATTTGTAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAGCTCTAGAGCAAGTCTTGCATCATGCAGCACTCAATTTGTTTATTGGTTGATTCTAGTTGTAAACCTTAACGATAGATGTTCACGTATGATTACTTCAAGAAGCTGGACTACTTCTCCACCGAGTGTAAGTTTTTTTTTCTTTTAGTTGTCTTTTTTGTTTTATTTTATTTTATTTTCCACTCTTCTGCATCTTACTAATTGATAAGAAGTACAAATTCATTCTGTGCATTTTACATGGCTAGGCATAATTAGCTATAAAATTTGATTGATTGAATGTCAAGTTGTGATCGGTCAATAATTAACTATGAAATTTGTGTCCTAAGATTTCTTGTTTTTCATGTGGTTTCTTGTCATACCCTATTTAACTTTTTGGTTTGATTTTGTTTACGTGAAGAGTCTTTCTTTGTTTTGAGAATTTGTTGAAGAAGTTTAATATTTGTTATGTGGAGATTTGAAGATGTTGATTTTGTTTATGTGGAGATGTGTACGTATATATACTTGTTATGTGGAGATTTGTAGAGTTGATTTTGTTTATGTGGAGATTTGCAAAATTTTATAATTTGAACACTTTGGATCTTTTGGTTAAGATTTAGACGATAATTTTGTTCTAATTTTTTTTGGATTAAATTTCAGTGTTTTAGAATTGTCTCTAACATTTGCTTATTCTTTCTCTAATTAAGTAACTAATAAATATTTTTTTCCTTCTCTCACAGAATTCAAGAACTTTCCAAGTTTCAACAAATTAGAGACTTTGTTGAAGTTGTTTGAATGAATCTATCACTTTACATGGTTAGTTGTTGCTTTATTCTTTCTATATAAGTTGCTTTATTATATATATGATGTAATGAAGAAGTGAGTTCAAAATTTTAGGTTACAAAAATTTAAAAAATAAAATAAAAGTAATAATGGAATGTATTTTTTCTTAAAAAAATGATCGAACACCTGATGCACAAATATTAGGCGTCGGGAGTTTATAATTCCCGACACGTTG

mRNA sequence

GAACCTTTCCCCGAAGGGTGTCCAACAGCTGCTTTTGGAAGTTGGCAATAGATCCTCGACCACCCTACTTCAGTTGAAGAACTTCTATCTCTTCTCGACAAAATTGAGCGTTTGCTGGCAAAAGTTAAACAATCCCCTTCAAAATCAATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAGCTCTAGAGCAAGTCTTGCATCATGCAGCACTCAATTTGTTTATTGGTTGATTCTAGTTGTAAACCTTAACGATAGATGTTCACGTATGATTACTTCAAGAAGCTGGACTACTTCTCCACCGAGTAATTCAAGAACTTTCCAAGTTTCAACAAATTAGAGACTTTGTTGAAGTTGTTTGAATGAATCTATCACTTTACATGGTTAGTTGTTGCTTTATTCTTTCTATATAAGTTGCTTTATTATATATATGATGTAATGAAGAAGTGAGTTCAAAATTTTAGGTTACAAAAATTTAAAAAATAAAATAAAAGTAATAATGGAATGTATTTTTTCTTAAAAAAATGATCGAACACCTGATGCACAAATATTAGGCGTCGGGAGTTTATAATTCCCGACACGTTG

Coding sequence (CDS)

ATGCTAATTGCGCTCACTCCATCACAGAATGCCTTGGTTTCTGATCGACTTCTTAGGCATTCAAATATTAATGTCAAAGTTGCAGTTGCAGCATGCGTTAGTGAAATAACTCGGATCATTGCACCTAATGCCCCTTACAGTGATGGAAATATGAAGAAGGCTCTTGATCGAGGTGCTGCATTGTTGAAAGTAGTCAAGCTTGCCACAAGGCACAATGCAATTGATATGGCTGAAACAGTTCTATTAGATATATTACGAGGTGATATTGCCAGATTGAGTAGATGTACTGCAATAAGTACCGATGAAGATGGACCAATTCCAAGATGCAAACCTTTCAAGTACACATATGAAAAGAAGATAGTAGTATATTTTTATATTTTAGTCTCATAG

Protein sequence

MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFYILVS
Homology
[Close]
BLAST of MELO3C004787.2 vs. NCBI nr
Match: XP_010063105.1 (PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Eucalyptus grandis] >KCW70297.1 hypothetical protein EUGRSUZ_F03540 [Eucalyptus grandis])

HSP 1 Score: 127.5 bits (319), Expect = 3.3e-26
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 139 MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIA 198

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 199 RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 233

[Close]
BLAST of MELO3C004787.2 vs. NCBI nr
Match: XP_011649473.1 (PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Cucumis sativus] >KGN62283.1 hypothetical protein Csa_2G348210 [Cucumis sativus])

HSP 1 Score: 126.7 bits (317), Expect = 5.7e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787.2 vs. NCBI nr
Match: XP_022144278.1 (cytoplasmic tRNA 2-thiolation protein 1 [Momordica charantia])

HSP 1 Score: 126.7 bits (317), Expect = 5.7e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787.2 vs. NCBI nr
Match: XP_022951723.1 (cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita moschata])

HSP 1 Score: 126.7 bits (317), Expect = 5.7e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787.2 vs. NCBI nr
Match: XP_023002400.1 (cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita maxima])

HSP 1 Score: 126.7 bits (317), Expect = 5.7e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787.2 vs. TAIR10
Match: AT1G76170.1 (2-thiocytidine tRNA biosynthesis protein, TtcA)

HSP 1 Score: 122.5 bits (306), Expect = 1.9e-28
Identity = 63/95 (66.32%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETV+L+ILRGDIA
Sbjct: 114 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLVTGHNADDIAETVVLNILRGDIA 173

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 174 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 208

[Close]
BLAST of MELO3C004787.2 vs. TAIR10
Match: AT2G44270.2 (repressor of lrx1)

HSP 1 Score: 108.6 bits (270), Expect = 2.9e-24
Identity = 56/86 (65.12%), Postives = 67/86 (77.91%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 146 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 205

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTY 117
           RLSRCT+I+T EDGPIPRCKPF +TY
Sbjct: 206 RLSRCTSITTGEDGPIPRCKPFNFTY 231

[Close]
BLAST of MELO3C004787.2 vs. TAIR10
Match: AT4G31880.1 (LOCATED IN: cytosol, chloroplast)

HSP 1 Score: 67.0 bits (162), Expect = 9.6e-12
Identity = 31/50 (62.00%), Postives = 39/50 (78.00%), Query Frame = 0 

Query: 4   ALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK 54
           ALTP    LV  +L +HS+++VKVAVAAC+SEITRI AP+APY D  MK+
Sbjct: 54  ALTPLMKGLVGGKLFKHSDVDVKVAVAACISEITRITAPDAPYDDDQMKE 103

[Close]
BLAST of MELO3C004787.2 vs. TAIR10
Match: AT1G80810.2 (Tudor/PWWP/MBT superfamily protein)

HSP 1 Score: 56.2 bits (134), Expect = 1.7e-08
Identity = 29/53 (54.72%), Postives = 38/53 (71.70%), Query Frame = 0 

Query: 1  MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKK 54
          M  AL PS+NALVS  LL H + +V+V+V +C++EI RI AP  PYSD  MK+
Sbjct: 1  MQSALIPSRNALVSVDLLSHPDSDVRVSVVSCLTEIVRITAPETPYSDDLMKE 53

[Close]
BLAST of MELO3C004787.2 vs. TAIR10
Match: AT5G47690.3 (binding)

HSP 1 Score: 54.3 bits (129), Expect = 6.5e-08
Identity = 26/52 (50.00%), Postives = 37/52 (71.15%), Query Frame = 0 

Query: 1  MLIALTPSQNALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMK 53
          +L ++ P  +A++   +L H + +VK+ VA+CVSEITRI AP APYSD  MK
Sbjct: 48 VLKSIQPFLDAVIKPEILNHQDKDVKLLVASCVSEITRITAPEAPYSDNIMK 99

[Close]
BLAST of MELO3C004787.2 vs. Swiss-Prot
Match: sp|O64862|CTU1_ARATH (Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-27
Identity = 64/95 (67.37%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KL T HNA D+AETVLL+ILRGDIA
Sbjct: 136 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIA 195

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 196 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 230

[Close]
BLAST of MELO3C004787.2 vs. Swiss-Prot
Match: sp|Q6Z6G6|CTU1_ORYSJ (Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCS6 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.0e-26
Identity = 59/74 (79.73%), Postives = 67/74 (90.54%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGAALLKV K+ T HNA D+AETVLL+ILRGDIARLSRCT I+T EDGPIPRCKP
Sbjct: 163 RQALDRGAALLKVDKIVTGHNADDIAETVLLNILRGDIARLSRCTFITTGEDGPIPRCKP 222

Query: 112 FKYTYEKKIVVYFY 126
           FKYTYEK+IV+Y Y
Sbjct: 223 FKYTYEKEIVMYAY 236

[Close]
BLAST of MELO3C004787.2 vs. Swiss-Prot
Match: sp|Q05AW7|CTU1_XENLA (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 9.8e-22
Identity = 49/74 (66.22%), Postives = 61/74 (82.43%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGA +L + K+ T HNA D+AETVL++ LRGDIARL RCTAI+T  +G IPRCKP
Sbjct: 148 RQALDRGAMMLGINKICTGHNADDIAETVLMNFLRGDIARLRRCTAITTGSEGAIPRCKP 207

Query: 112 FKYTYEKKIVVYFY 126
            KYTYEK+IV+Y Y
Sbjct: 208 LKYTYEKEIVLYAY 221

[Close]
BLAST of MELO3C004787.2 vs. Swiss-Prot
Match: sp|Q803X1|CTU1_DANRE (Cytoplasmic tRNA 2-thiolation protein 1 OS=Danio rerio OX=7955 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.9e-21
Identity = 51/76 (67.11%), Postives = 62/76 (81.58%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAIST--DEDGPIPRC 111
           ++ALDRGA +L V K+ T HNA D+AETVL+++LRGDIARL RCTAIST  + DG IPRC
Sbjct: 148 RQALDRGAMMLNVDKICTGHNADDVAETVLMNVLRGDIARLRRCTAISTSSEGDGAIPRC 207

Query: 112 KPFKYTYEKKIVVYFY 126
           KP KY YEK+IV+Y Y
Sbjct: 208 KPLKYAYEKEIVLYAY 223

[Close]
BLAST of MELO3C004787.2 vs. Swiss-Prot
Match: sp|Q5FW05|CTU1_XENTR (Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 6.4e-21
Identity = 48/74 (64.86%), Postives = 60/74 (81.08%), Query Frame = 0 

Query: 52  KKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKP 111
           ++ALDRGA +L V K+ T HNA D+AETVL++ LRGDIARL RCT+I+T  +G IPRCKP
Sbjct: 148 RQALDRGAMMLGVNKICTGHNADDIAETVLMNFLRGDIARLRRCTSITTGSEGAIPRCKP 207

Query: 112 FKYTYEKKIVVYFY 126
            KY YEK+IV+Y Y
Sbjct: 208 LKYAYEKEIVLYAY 221

[Close]
BLAST of MELO3C004787.2 vs. TrEMBL
Match: tr|A0A059BW08|A0A059BW08_EUCGR (Cytoplasmic tRNA 2-thiolation protein 1 OS=Eucalyptus grandis OX=71139 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.2e-26
Identity = 67/95 (70.53%), Postives = 77/95 (81.05%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI +II    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 139 MDEIVKIIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIA 198

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 199 RLSRCTAITTGEDGPIPRCKPFKYTYEKEIVMYAY 233

[Close]
BLAST of MELO3C004787.2 vs. TrEMBL
Match: tr|A0A0A0LKF0|A0A0A0LKF0_CUCSA (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-26
Identity = 67/95 (70.53%), Postives = 76/95 (80.00%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 138 MDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDMAETVLLNILRGDIA 197

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCTAI T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 198 RLSRCTAIITGEDGPIPRCKPFKYTYEKEIVMYAY 232

[Close]
BLAST of MELO3C004787.2 vs. TrEMBL
Match: tr|A0A2R6PZE9|A0A2R6PZE9_ACTCH (Cytoplasmic tRNA 2-thiolation protein 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=NCS6 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 8.3e-26
Identity = 65/95 (68.42%), Postives = 77/95 (81.05%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGAALLKV KLAT HNA D+AETVLL+ILRGDIA
Sbjct: 137 MDEIVKLIGLKNNCTFCGVFRRQALDRGAALLKVDKLATGHNADDIAETVLLNILRGDIA 196

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           RLSRCT+I+T EDGPIPRCKPFKYTYEK+IV+Y Y
Sbjct: 197 RLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAY 231

[Close]
BLAST of MELO3C004787.2 vs. TrEMBL
Match: tr|A0A151SFJ5|A0A151SFJ5_CAJCA (Cytoplasmic tRNA 2-thiolation protein 1 OS=Cajanus cajan OX=3821 GN=KK1_024469 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-25
Identity = 67/115 (58.26%), Postives = 83/115 (72.17%), Query Frame = 0 

Query: 11  ALVSDRLLRHSNINVKVAVAACVSEITRIIAPNAPYSDGNMKKALDRGAALLKVVKLATR 70
           A V  +L RH N  + + + +    IT        Y D +++ ALDRGAALLKV KL T 
Sbjct: 74  AYVLSKLNRHHNYGLHLFLLSVDEGIT-------GYRDDSLQTALDRGAALLKVDKLVTG 133

Query: 71  HNAIDMAETVLLDILRGDIARLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFY 126
           HNA D+AETVLL++LRGD+ARLSRCT+I+T EDG IPRCKPFKYTYEK+IV+Y Y
Sbjct: 134 HNADDIAETVLLNLLRGDVARLSRCTSITTGEDGAIPRCKPFKYTYEKEIVIYPY 181

[Close]
BLAST of MELO3C004787.2 vs. TrEMBL
Match: tr|A0A2I0AFW8|A0A2I0AFW8_9ASPA (Cytoplasmic tRNA 2-thiolation protein 1 OS=Apostasia shenzhenica OX=1088818 GN=NCS6 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-25
Identity = 66/97 (68.04%), Postives = 76/97 (78.35%), Query Frame = 0 

Query: 33  VSEITRIIA--PNAPYSDGNMKKALDRGAALLKVVKLATRHNAIDMAETVLLDILRGDIA 92
           + EI ++I    N  +     ++ALDRGA LLKV KLAT HNA DMAETVLL+ILRGDIA
Sbjct: 140 MDEIVKVIGLKNNCTFCGVFRRQALDRGATLLKVDKLATGHNADDMAETVLLNILRGDIA 199

Query: 93  RLSRCTAISTDEDGPIPRCKPFKYTYEKKIVVYFYIL 128
           RLSRCT I T EDGPIPRCKPFKYTYEK+IV+Y +IL
Sbjct: 200 RLSRCTYIVTGEDGPIPRCKPFKYTYEKEIVIYPFIL 236

The following BLAST results are available for this feature:
BLAST of MELO3C004787.2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_010063105.13.3e-2670.53PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Eucalyptus grandis] >KCW7029... [more]
XP_011649473.15.7e-2670.53PREDICTED: cytoplasmic tRNA 2-thiolation protein 1 [Cucumis sativus] >KGN62283.1... [more]
XP_022144278.15.7e-2670.53cytoplasmic tRNA 2-thiolation protein 1 [Momordica charantia][more]
XP_022951723.15.7e-2670.53cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita moschata][more]
XP_023002400.15.7e-2670.53cytoplasmic tRNA 2-thiolation protein 1 [Cucurbita maxima][more]
BLAST of MELO3C004787.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G76170.11.9e-2866.322-thiocytidine tRNA biosynthesis protein, TtcA[more]
AT2G44270.22.9e-2465.12repressor of lrx1[more]
AT4G31880.19.6e-1262.00LOCATED IN: cytosol, chloroplast[more]
AT1G80810.21.7e-0854.72Tudor/PWWP/MBT superfamily protein[more]
AT5G47690.36.5e-0850.00binding[more]
BLAST of MELO3C004787.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O64862|CTU1_ARATH1.2e-2767.37Cytoplasmic tRNA 2-thiolation protein 1 OS=Arabidopsis thaliana OX=3702 GN=NCS6 ... [more]
sp|Q6Z6G6|CTU1_ORYSJ1.0e-2679.73Cytoplasmic tRNA 2-thiolation protein 1 OS=Oryza sativa subsp. japonica OX=39947... [more]
sp|Q05AW7|CTU1_XENLA9.8e-2266.22Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus laevis OX=8355 GN=ctu1 PE=2 S... [more]
sp|Q803X1|CTU1_DANRE2.9e-2167.11Cytoplasmic tRNA 2-thiolation protein 1 OS=Danio rerio OX=7955 GN=ctu1 PE=2 SV=1[more]
sp|Q5FW05|CTU1_XENTR6.4e-2164.86Cytoplasmic tRNA 2-thiolation protein 1 OS=Xenopus tropicalis OX=8364 GN=ctu1 PE... [more]
BLAST of MELO3C004787.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A059BW08|A0A059BW08_EUCGR2.2e-2670.53Cytoplasmic tRNA 2-thiolation protein 1 OS=Eucalyptus grandis OX=71139 GN=NCS6 P... [more]
tr|A0A0A0LKF0|A0A0A0LKF0_CUCSA3.7e-2670.53Cytoplasmic tRNA 2-thiolation protein 1 OS=Cucumis sativus OX=3659 GN=NCS6 PE=3 ... [more]
tr|A0A2R6PZE9|A0A2R6PZE9_ACTCH8.3e-2668.42Cytoplasmic tRNA 2-thiolation protein 1 OS=Actinidia chinensis var. chinensis OX... [more]
tr|A0A151SFJ5|A0A151SFJ5_CAJCA1.1e-2558.26Cytoplasmic tRNA 2-thiolation protein 1 OS=Cajanus cajan OX=3821 GN=KK1_024469 P... [more]
tr|A0A2I0AFW8|A0A2I0AFW8_9ASPA1.4e-2568.04Cytoplasmic tRNA 2-thiolation protein 1 OS=Apostasia shenzhenica OX=1088818 GN=N... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0000049tRNA binding
Vocabulary: Biological Process
TermDefinition
GO:0034227tRNA thio-modification
GO:0002098tRNA wobble uridine modification
Vocabulary: INTERPRO
TermDefinition
IPR000541Ncs6/Tuc1/Ctu1
IPR014729Rossmann-like_a/b/a_fold
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032447 protein urmylation
biological_process GO:0008033 tRNA processing
biological_process GO:0034227 tRNA thio-modification
biological_process GO:0002098 tRNA wobble uridine modification
biological_process GO:0008150 biological_process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C004787.2.1MELO3C004787.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 15..126
e-value: 3.9E-11
score: 44.6
NoneNo IPR availablePANTHERPTHR11807ATPASES OF THE PP SUPERFAMILY-RELATEDcoord: 52..125
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 53..124
IPR000541Cytoplasmic tRNA 2-thiolation protein 1PANTHERPTHR11807:SF12CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1coord: 52..125

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
MELO3C004787.2CSPI01G14370Wild cucumber (PI 183967)cpimedB011
MELO3C004787.2Lsi03G000940Bottle gourd (USVL1VR-Ls)lsimedB255
MELO3C004787.2Bhi08G000076Wax gourdmedwgoB176
MELO3C004787.2CsaV3_1G014540Cucumber (Chinese Long) v3cucmedB009
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
MELO3C004787.2Cucumber (Gy14) v2cgybmedB009
MELO3C004787.2Cucumber (Gy14) v1cgymedB361
MELO3C004787.2Cucurbita maxima (Rimu)cmamedB161
MELO3C004787.2Cucurbita maxima (Rimu)cmamedB215
MELO3C004787.2Cucurbita moschata (Rifu)cmomedB151
MELO3C004787.2Cucurbita moschata (Rifu)cmomedB203
MELO3C004787.2Cucurbita pepo (Zucchini)cpemedB557
MELO3C004787.2Cucurbita pepo (Zucchini)cpemedB881
MELO3C004787.2Cucumber (Chinese Long) v2cumedB010
MELO3C004787.2Watermelon (Charleston Gray)medwcgB133
MELO3C004787.2Watermelon (97103) v1medwmB155
MELO3C004787.2Watermelon (97103) v2medwmbB132