MELO3C002888 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C002888
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Transcription initiation factor IIF subunit beta, putative
Location	chr9 : 7013238 .. 7014469 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCCACATGATCAGAATCTTGAGAGGTATGGGAAACTCTGTCGTGAAAGAACCCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTCTCCTTTCTAACTTCTTATGCAGCGTACTTGGGTGTTTGGTATCTTTCTTTATTGCTGTCACGTTCTCTTGGTTAACATTTCCAAATATTCTATATAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCTATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGTAATTATACTACCTTCATTTCTTCTTTGATAACATTATCAAGTTAAATTTTGATTTCGCCTGTGCTGTATTACATTCCCTCGTAGCCAGTCAGAAGTTTGTCCAAATGCTTTCAAGAATTTCTTTTAAGATGCTAATTTTGGTACAGGAGAAGAAGAAAGTGGTGAGCAAGGGATCGGAAACAAAGCGGTTGAGAAAGGAGCGTGGAGAATTGGAGAAGATTATTTTTAAGCTTTTCGAAAGACAGCCATATTGGACGTCAAAACAATTGATTCAAGAGACTGACCAACCTGAAGTAAGATTTATCTTTGACCTCTTTTTCTTTCTTTTATATTATTTTATTATTTTATTTTTATGAAACAAAGAAAGTGACTTATCACAAAAGGAATACAGTCTATAGAATCGAAGGGTTGGAAGGGTGAAAAACTCCTTCCCTCAGATTAAACTTTGGGCTAAGCTTAGTTACAAAATTTTTCTTGTTTTCATCTCTCTTAGGGTAGAGTTCGATTGTACACCTATCGAATACTAAGCTTGCATGTATAGTGTACCATGTAGTTACTAAACTTATGACTAGAGTCTTGCATTGTTTATAGGACCTGAACATAATTCTGGAATATAAAATAAATTGTACATTATATTCACTCCGTATTTTTCATTTAGAAAAAGGTCACACATCTCTCAAACATAAGCAATATGTATCCTTGGGAAAAATGCAAATGGACTGCTCACCTGAAACACTGCAGTTCTAGTTGATCTCTTGAATCTGCTTCTTGTTAGGCTACCACTAGGTTTGATTAAAGCCTGAAACATCCATTATTTCTTTCTTTCTTTTTTCTTTTTTGATTTGCAGCAATATATGAAGGAAATACTTAAAGACCTTTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCAAGTGAGGAAACAAAACCACGATAG

mRNA sequence

ATGAAGCCACATGATCAGAATCTTGAGAGGTATGGGAAACTCTGTCGTGAAAGAACCCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCTATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAAGTGGTGAGCAAGGGATCGGAAACAAAGCGGTTGAGAAAGGAGCGTGGAGAATTGGAGAAGATTATTTTTAAGCTTTTCGAAAGACAGCCATATTGGACGTCAAAACAATTGATTCAAGAGACTGACCAACCTGAACAATATATGAAGGAAATACTTAAAGACCTTTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCAAGTGAGGAAACAAAACCACGATAG

Coding sequence (CDS)

ATGAAGCCACATGATCAGAATCTTGAGAGGTATGGGAAACTCTGTCGTGAAAGAACCCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCTATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAAGTGGTGAGCAAGGGATCGGAAACAAAGCGGTTGAGAAAGGAGCGTGGAGAATTGGAGAAGATTATTTTTAAGCTTTTCGAAAGACAGCCATATTGGACGTCAAAACAATTGATTCAAGAGACTGACCAACCTGAACAATATATGAAGGAAATACTTAAAGACCTTTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCAAGTGAGGAAACAAAACCACGATAG

Protein sequence

MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKPEYKESSEETKPR*

Homology

[Close]

BLAST of MELO3C002888 vs. Swiss-Prot
Match: T2FB_XENLA (General transcription factor IIF subunit 2 OS=Xenopus laevis GN=gtf2f2 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.8e-14
Identity = 42/132 (31.82%), Postives = 70/132 (53.03%), Query Frame = 1


Query: 9   ERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKR 68
           + Y ++ R++  +S    RQ Q ++     + +P+       +    +KK   K  + KR
Sbjct: 137 DNYMQMKRKQIEESSKPKRQSQQLEKAVTSNYKPVSNHQ---YNIEYEKK---KKDDGKR 196

Query: 69  LRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYEL 128
            R ++ ++  ++F  FE+  Y+  K L+  T QP  Y+KEIL+D+ +YN KG H+ T+EL
Sbjct: 197 ARVDKHQVLDMLFSAFEKHQYYNIKDLVDITKQPVTYLKEILRDIGIYNMKGTHKNTWEL 256

Query: 129 KPEYKESSEETK 141
           KPEY+    E K
Sbjct: 257 KPEYRHYQGEDK 262

[Close]

BLAST of MELO3C002888 vs. Swiss-Prot
Match: T2FB_MOUSE (General transcription factor IIF subunit 2 OS=Mus musculus GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.1e-13
Identity = 45/132 (34.09%), Postives = 67/132 (50.76%), Query Frame = 1


Query: 9   ERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKR 68
           E Y KL R +  +S    R  Q +D V   + +P+      +     KKK      + KR
Sbjct: 122 ENYMKLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQY-NIEYERKKK-----EDGKR 181

Query: 69  LRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYEL 128
            R ++  +  ++F  FE+  Y+  K L+  T QP  Y+KEILK++ + N KG+H+ T+EL
Sbjct: 182 ARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTWEL 241

Query: 129 KPEYKESSEETK 141
           KPEY+    E K
Sbjct: 242 KPEYRHYQTEEK 247

[Close]

BLAST of MELO3C002888 vs. Swiss-Prot
Match: T2FB_RAT (General transcription factor IIF subunit 2 OS=Rattus norvegicus GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 7.0e-13
Identity = 45/132 (34.09%), Postives = 66/132 (50.00%), Query Frame = 1


Query: 9   ERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKR 68
           E Y KL R +  +S    R  Q  D V   + +P+      +     KKK      + KR
Sbjct: 122 ENYMKLKRLQIEESSKPVRLSQQADKVVTTNYKPVANHQY-NIEYERKKK-----EDGKR 181

Query: 69  LRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYEL 128
            R ++  +  ++F  FE+  Y+  K L+  T QP  Y+KEILK++ + N KG+H+ T+EL
Sbjct: 182 ARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTWEL 241

Query: 129 KPEYKESSEETK 141
           KPEY+    E K
Sbjct: 242 KPEYRHYQTEEK 247

[Close]

BLAST of MELO3C002888 vs. Swiss-Prot
Match: T2FB_HUMAN (General transcription factor IIF subunit 2 OS=Homo sapiens GN=GTF2F2 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 7.0e-13
Identity = 45/132 (34.09%), Postives = 67/132 (50.76%), Query Frame = 1


Query: 9   ERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKR 68
           E Y +L R +  +S    R  Q +D V   + +P+      +     KKK      + KR
Sbjct: 122 ENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQY-NIEYERKKK-----EDGKR 181

Query: 69  LRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYEL 128
            R ++  +  ++F  FE+  Y+  K L+  T QP  Y+KEILK++ V N KG+H+ T+EL
Sbjct: 182 ARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWEL 241

Query: 129 KPEYKESSEETK 141
           KPEY+    E K
Sbjct: 242 KPEYRHYQGEEK 247

[Close]

BLAST of MELO3C002888 vs. Swiss-Prot
Match: T2FB_DROME (General transcription factor IIF subunit 2 OS=Drosophila melanogaster GN=TfIIFbeta PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 9.1e-13
Identity = 44/130 (33.85%), Postives = 68/130 (52.31%), Query Frame = 1


Query: 11  YGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLR 70
           Y KL  E   K+    R++Q ID +   + +P+      +    E+KK     +E K+ R
Sbjct: 142 YMKLKLESIRKASEPQRRVQPIDKIVQ-NFKPVKD-HAHNIEYRERKK-----AEGKKAR 201

Query: 71  KERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKGVHQGTYELKP 130
            ++  +  ++F  FE+  Y+  K L++ T+QP  Y+KEILKD+C YN K  H+  +ELK 
Sbjct: 202 DDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDVCDYNMKNPHKNMWELKK 261

Query: 131 EYKESSEETK 141
           EY+    E K
Sbjct: 262 EYRHYKTEEK 264

[Close]

BLAST of MELO3C002888 vs. TrEMBL
Match: A0A0A0KQP0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G492360 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 4.0e-76
Identity = 140/142 (98.59%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV
Sbjct: 49  MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 108

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG
Sbjct: 109 SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 168

Query: 121 VHQGTYELKPEYKESSEETKPR 143
           VHQGTYELKPEY+ESSE+TKPR
Sbjct: 169 VHQGTYELKPEYRESSEDTKPR 190

[Close]

BLAST of MELO3C002888 vs. TrEMBL
Match: F6HNI2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g03980 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.1e-52
Identity = 98/142 (69.01%), Postives = 123/142 (86.62%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPH++N++ YGKLCRERT K MTKSRQIQVID+  G HMRPMPGM V++   ++KKK+ 
Sbjct: 117 MKPHNENIQNYGKLCRERTSKYMTKSRQIQVIDNDNGSHMRPMPGM-VIASAVSDKKKMP 176

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           +KGS+ KR R++RGE+E+I+FKLFERQP WT +QLIQETDQPEQ++K++LKDLCVYNNKG
Sbjct: 177 TKGSDMKRTRRDRGEMEEILFKLFERQPNWTLRQLIQETDQPEQFLKDLLKDLCVYNNKG 236

Query: 121 VHQGTYELKPEYKESSEETKPR 143
            +QGTYELKPEYK S EE+ P+
Sbjct: 237 TNQGTYELKPEYKRSGEESTPK 257

[Close]

BLAST of MELO3C002888 vs. TrEMBL
Match: A0A061FUV7_THECC (JHL18I08.10 protein isoform 1 OS=Theobroma cacao GN=TCM_043099 PE=4 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.0e-51
Identity = 99/141 (70.21%), Postives = 120/141 (85.11%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           M+PHD+N+E YGKLCRERT+K MTKSRQIQVID+  G HMRPMPGM + +    EKKK  
Sbjct: 123 MRPHDENIENYGKLCRERTNKYMTKSRQIQVIDNDNGTHMRPMPGMMIATV-FNEKKKTP 182

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           +K SETKR R++RGE+E I+FKLFERQP WT +QLIQETDQPEQ++K+ILKDLCVYNNKG
Sbjct: 183 TKTSETKRTRRDRGEMEDIMFKLFERQPNWTLRQLIQETDQPEQFLKDILKDLCVYNNKG 242

Query: 121 VHQGTYELKPEYKESSEETKP 142
            +QG+YELKPEYK++S +T P
Sbjct: 243 TNQGSYELKPEYKKASNDTNP 262

[Close]

BLAST of MELO3C002888 vs. TrEMBL
Match: A0A061FMN6_THECC (Transcription initiation factor IIF isoform 2 OS=Theobroma cacao GN=TCM_043099 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 5.0e-50
Identity = 99/142 (69.72%), Postives = 120/142 (84.51%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQ-VIDHVTGGHMRPMPGMDVLSFGAAEKKKV 60
           M+PHD+N+E YGKLCRERT+K MTKSRQIQ VID+  G HMRPMPGM + +    EKKK 
Sbjct: 123 MRPHDENIENYGKLCRERTNKYMTKSRQIQQVIDNDNGTHMRPMPGMMIATV-FNEKKKT 182

Query: 61  VSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNK 120
            +K SETKR R++RGE+E I+FKLFERQP WT +QLIQETDQPEQ++K+ILKDLCVYNNK
Sbjct: 183 PTKTSETKRTRRDRGEMEDIMFKLFERQPNWTLRQLIQETDQPEQFLKDILKDLCVYNNK 242

Query: 121 GVHQGTYELKPEYKESSEETKP 142
           G +QG+YELKPEYK++S +T P
Sbjct: 243 GTNQGSYELKPEYKKASNDTNP 263

[Close]

BLAST of MELO3C002888 vs. TrEMBL
Match: A0A0D2UXJ1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G240900 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 6.5e-50
Identity = 96/141 (68.09%), Postives = 119/141 (84.40%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           M+PHD+N+E YGKLCRERT KSM KSRQIQVID+  G HMRPMPGM +++    EKKK  
Sbjct: 123 MRPHDENIENYGKLCRERTTKSMNKSRQIQVIDNDNGTHMRPMPGM-IIAAVFNEKKKAP 182

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           +K S+TKR R++RGE+E I+FKLFERQ  WT +QLIQETDQPEQ++K+ILKDLC+YNNKG
Sbjct: 183 AKTSDTKRTRRDRGEMEDIMFKLFERQSNWTLRQLIQETDQPEQFLKDILKDLCIYNNKG 242

Query: 121 VHQGTYELKPEYKESSEETKP 142
            +QGTYELKPEYK++++ T P
Sbjct: 243 TNQGTYELKPEYKKATDGTNP 262

[Close]

BLAST of MELO3C002888 vs. TAIR10
Match: AT3G52270.1 (AT3G52270.1 Transcription initiation factor IIF, beta subunit)

HSP 1 Score: 189.9 bits (481), Expect = 1.1e-48
Identity = 86/138 (62.32%), Postives = 114/138 (82.61%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           M+PH++N+E YG+LCRERT+K M K+RQIQVID+  G HMRPMPGM ++   A EKKK+ 
Sbjct: 133 MRPHNENIESYGRLCRERTNKYMGKNRQIQVIDNARGMHMRPMPGM-IIPTAAPEKKKLT 192

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           ++ SE KR R++R E+E+++F LFERQ  WT + LIQETDQPEQ++K++LKDLC+YNNKG
Sbjct: 193 NRTSEMKRTRRDRREMEEVMFNLFERQSNWTLRLLIQETDQPEQFLKDLLKDLCIYNNKG 252

Query: 121 VHQGTYELKPEYKESSEE 139
            +QGTYELKPEYK++++E
Sbjct: 253 SNQGTYELKPEYKKATQE 269

[Close]

BLAST of MELO3C002888 vs. TAIR10
Match: AT1G75510.1 (AT1G75510.1 Transcription initiation factor IIF, beta subunit)

HSP 1 Score: 178.3 bits (451), Expect = 3.3e-45
Identity = 87/140 (62.14%), Postives = 109/140 (77.86%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGM-DVLSFGAAEKKKV 60
           MKP+ + +E Y +LCRERT K+M K+RQIQVID+  G HMRPMPGM  ++S  + EK+K 
Sbjct: 117 MKPYGETIEEYARLCRERTSKAMVKNRQIQVIDNDRGVHMRPMPGMLGLVSSNSKEKRKP 176

Query: 61  VS-KGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNN 120
              K +E KR R++RGELE I+FKLFE QP WT KQL+QETDQP Q++KEIL +LCVYN 
Sbjct: 177 PPVKQTEVKRTRRDRGELEAIMFKLFEGQPNWTLKQLVQETDQPAQFLKEILNELCVYNK 236

Query: 121 KGVHQGTYELKPEYKESSEE 139
           +G +QGTYELKPEYK+S+E+
Sbjct: 237 RGSNQGTYELKPEYKKSAED 256

[Close]

BLAST of MELO3C002888 vs. NCBI nr
Match: gi|659131396|ref|XP_008465664.1| (PREDICTED: general transcription factor IIF subunit 2 isoform X1 [Cucumis melo])

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-76
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV
Sbjct: 142 MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 201

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG
Sbjct: 202 SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 261

Query: 121 VHQGTYELKPEYKESSEETKPR 143
           VHQGTYELKPEYKESSEETKPR
Sbjct: 262 VHQGTYELKPEYKESSEETKPR 283

[Close]

BLAST of MELO3C002888 vs. NCBI nr
Match: gi|659131398|ref|XP_008465665.1| (PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo])

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-76
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV
Sbjct: 136 MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 195

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG
Sbjct: 196 SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 255

Query: 121 VHQGTYELKPEYKESSEETKPR 143
           VHQGTYELKPEYKESSEETKPR
Sbjct: 256 VHQGTYELKPEYKESSEETKPR 277

[Close]

BLAST of MELO3C002888 vs. NCBI nr
Match: gi|700196037|gb|KGN51214.1| (hypothetical protein Csa_5G492360 [Cucumis sativus])

HSP 1 Score: 292.0 bits (746), Expect = 5.8e-76
Identity = 140/142 (98.59%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV
Sbjct: 49  MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 108

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG
Sbjct: 109 SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 168

Query: 121 VHQGTYELKPEYKESSEETKPR 143
           VHQGTYELKPEY+ESSE+TKPR
Sbjct: 169 VHQGTYELKPEYRESSEDTKPR 190

[Close]

BLAST of MELO3C002888 vs. NCBI nr
Match: gi|449452114|ref|XP_004143805.1| (PREDICTED: general transcription factor IIF subunit 2 [Cucumis sativus])

HSP 1 Score: 292.0 bits (746), Expect = 5.8e-76
Identity = 140/142 (98.59%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV
Sbjct: 136 MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 195

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG
Sbjct: 196 SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 255

Query: 121 VHQGTYELKPEYKESSEETKPR 143
           VHQGTYELKPEY+ESSE+TKPR
Sbjct: 256 VHQGTYELKPEYRESSEDTKPR 277

[Close]

BLAST of MELO3C002888 vs. NCBI nr
Match: gi|296086049|emb|CBI31490.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-52
Identity = 98/142 (69.01%), Postives = 123/142 (86.62%), Query Frame = 1


Query: 1   MKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVV 60
           MKPH++N++ YGKLCRERT K MTKSRQIQVID+  G HMRPMPGM V++   ++KKK+ 
Sbjct: 160 MKPHNENIQNYGKLCRERTSKYMTKSRQIQVIDNDNGSHMRPMPGM-VIASAVSDKKKMP 219

Query: 61  SKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQPEQYMKEILKDLCVYNNKG 120
           +KGS+ KR R++RGE+E+I+FKLFERQP WT +QLIQETDQPEQ++K++LKDLCVYNNKG
Sbjct: 220 TKGSDMKRTRRDRGEMEEILFKLFERQPNWTLRQLIQETDQPEQFLKDLLKDLCVYNNKG 279

Query: 121 VHQGTYELKPEYKESSEETKPR 143
            +QGTYELKPEYK S EE+ P+
Sbjct: 280 TNQGTYELKPEYKRSGEESTPK 300

The following BLAST results are available for this feature:

BLAST of MELO3C002888 vs. Swiss-Prot


Match Name	E-value	Identity	Description
T2FB_XENLA	2.8e-14	31.82	General transcription factor IIF subunit 2 OS=Xenopus laevis GN=gtf2f2 PE=2 SV=1	[more]
T2FB_MOUSE	3.1e-13	34.09	General transcription factor IIF subunit 2 OS=Mus musculus GN=Gtf2f2 PE=1 SV=1	[more]
T2FB_RAT	7.0e-13	34.09	General transcription factor IIF subunit 2 OS=Rattus norvegicus GN=Gtf2f2 PE=1 S...	[more]
T2FB_HUMAN	7.0e-13	34.09	General transcription factor IIF subunit 2 OS=Homo sapiens GN=GTF2F2 PE=1 SV=2	[more]
T2FB_DROME	9.1e-13	33.85	General transcription factor IIF subunit 2 OS=Drosophila melanogaster GN=TfIIFbe...	[more]

BLAST of MELO3C002888 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQP0_CUCSA	4.0e-76	98.59	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G492360 PE=4 SV=1	[more]
F6HNI2_VITVI	1.1e-52	69.01	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g03980 PE=4 SV=...	[more]
A0A061FUV7_THECC	2.0e-51	70.21	JHL18I08.10 protein isoform 1 OS=Theobroma cacao GN=TCM_043099 PE=4 SV=1	[more]
A0A061FMN6_THECC	5.0e-50	69.72	Transcription initiation factor IIF isoform 2 OS=Theobroma cacao GN=TCM_043099 P...	[more]
A0A0D2UXJ1_GOSRA	6.5e-50	68.09	Uncharacterized protein OS=Gossypium raimondii GN=B456_011G240900 PE=4 SV=1	[more]

BLAST of MELO3C002888 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G52270.1	1.1e-48	62.32	Transcription initiation factor IIF, beta subunit	[more]
AT1G75510.1	3.3e-45	62.14	Transcription initiation factor IIF, beta subunit	[more]

BLAST of MELO3C002888 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659131396\|ref\|XP_008465664.1\|	1.2e-76	100.00	PREDICTED: general transcription factor IIF subunit 2 isoform X1 [Cucumis melo]	[more]
gi\|659131398\|ref\|XP_008465665.1\|	1.2e-76	100.00	PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo]	[more]
gi\|700196037\|gb\|KGN51214.1\|	5.8e-76	98.59	hypothetical protein Csa_5G492360 [Cucumis sativus]	[more]
gi\|449452114\|ref\|XP_004143805.1\|	5.8e-76	98.59	PREDICTED: general transcription factor IIF subunit 2 [Cucumis sativus]	[more]
gi\|296086049\|emb\|CBI31490.3\|	1.5e-52	69.01	unnamed protein product [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003196	TFIIF_beta
IPR011991	Winged helix-turn-helix DNA-binding domain

Vocabulary: **Cellular Component**
Term	Definition
GO:0005674	transcription factor TFIIF complex

Vocabulary: **Biological Process**
Term	Definition
GO:0006366	transcription from RNA polymerase II promoter
GO:0006367	transcription initiation from RNA polymerase II promoter

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006446	regulation of translational initiation
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006366	transcription from RNA polymerase II promoter
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005674	transcription factor TFIIF complex
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C002888T1	MELO3C002888T1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003196	Transcription initiation factor IIF, beta subunit	PANTHER	PTHR10445	GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2	coord: 1..138 score: 6.0
IPR003196	Transcription initiation factor IIF, beta subunit	PFAM	PF02270	TFIIF_beta	coord: 7..133 score: 6.2
IPR011991	Winged helix-turn-helix DNA-binding domain	GENE3D	G3DSA:1.10.10.10		coord: 69..135 score: 3.8
IPR011991	Winged helix-turn-helix DNA-binding domain	unknown	SSF46785	"Winged helix" DNA-binding domain	coord: 69..134 score: 2.5
None	No IPR available	PANTHER	PTHR10445:SF2	TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT	coord: 1..138 score: 6.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C002888	Csa5G492360	Cucumber (Chinese Long) v2	cumeB361
MELO3C002888	Cla004738	Watermelon (97103) v1	mewmB003
MELO3C002888	ClCG09G020560	Watermelon (Charleston Gray)	mewcgB002
MELO3C002888	Cla97C09G181980	Watermelon (97103) v2	mewmbB038
MELO3C002888	Bhi06G000566	Wax gourd	mewgoB030
MELO3C002888	CSPI05G16780	Wild cucumber (PI 183967)	cpimeB350
MELO3C002888	Cucsa.275560	Cucumber (Gy14) v1	cgymeB460
MELO3C002888	CmaCh04G025910	Cucurbita maxima (Rimu)	cmameB634
MELO3C002888	CmoCh04G027130	Cucurbita moschata (Rifu)	cmomeB624
MELO3C002888	Lsi02G017100	Bottle gourd (USVL1VR-Ls)	lsimeB162
MELO3C002888	Cp4.1LG01g24180	Cucurbita pepo (Zucchini)	cpemeB358
MELO3C002888	CsGy5G016560	Cucumber (Gy14) v2	cgybmeB300
MELO3C002888	Carg04544	Silver-seed gourd	carmeB0682
MELO3C002888	CsaV3_5G025800	Cucumber (Chinese Long) v3	cucmeB359

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C002888	Cucurbita pepo (Zucchini)	cpemeB155
MELO3C002888	Silver-seed gourd	carmeB0260
MELO3C002888	Cucurbita maxima (Rimu)	cmameB251
MELO3C002888	Cucurbita moschata (Rifu)	cmomeB239