Lsi11G017130 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G017130
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Protein binding / ubiquitin-protein ligase/ zinc ion binding
Location	chr11 : 25248980 .. 25251157 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGACGTTCTACTAATTTTCCTCCAGGAATTTGGCAGGCCAGAAGGGAATATGATCGCCAGAGATATAACAGAAATGATTCAAACCATACGAACTCTACATCGTCGCATCCTCATTCATCTCAGGTCAGCGGGTTAACAATTCTTTTGTTTTGCAACAAACTGTATTCCAATCCCACGGCCACCATCAAAGCTTATGTTGTTATTCTACGCCATGATTTTCAGGCCATAAAACAGAACTTGTAGATCTAGATCACATTTAAATTTCCTGTTGATAGTTCTCCTAGTTTTCATATATTAGTACTTGAGAAGTTAAGGAGATGATAACAATCGTATTAGCTCATGCTCGTTCATATGTTGTCAGAATTCTTATAGAGAGATAGATCCAGTTCCATGGCGACCTGCTGGGGAGACAATGATTGCAAGAATATTGGACATCGGGAGGTAAATAACAAAACAAAAGAGCTTAATAGTTTTATAAGAGCTGTTTGGTGTTTTGGATTGAATTATAGGCAAACCAAAGAAGTTGGTATTGCAGATTCACTTCGTAAAATGATCTGAAATTAAGAGAATTTAGAGGCCAGGCAAAGTTTGCGGTACATTGCCTGAAAATAACATGAACTAGAACTAGTATCTAACTCAGGAAGATATAACTTGACAGAGAAGTCAGAAGTCCATGACTTTCACTAGTGGAAGAATTTGGGGAAACAAATCATCAAGTGTCAGAAATGGGGTTGAGCTCCAGTTCCTTCTTAGTTGGAGATTATTTTACACACGGGAGTTCAACAACATTCAACATCACACAATGCATTAAGCAGAAATTTGTTCAACAATAATCCACATTCAACATCACACAATGCATTAAGCAGAAATTTGTTCAACAATAATCCATATTACAATTTGCAGGGAAATAGAAATATGACATTCTCACTTGATTGAATTGCTTATGATATATAGCTATAACTTTTGAGAAGAATATATAGCAATAACTCTCAATTTTTGTCATGACTCATGAGCAGCATCCAAATTTGCAATAACAAACAAAATCCCCTTTGACAATATCAGTTGGTTTTAGAGGGAATCTACTTACATTGTCCTTATATCCTGCAAGTAAAGTCCATATCCTCCATACTTTGTTGATAATTCATTCACCTTTTGAGAAATTTCGAGCCCTCCAGAAGAACCACCTGCAATAGTCAGAATCATCACACACTCCAGACTGAGACAGCGTGAGGCCAGTCCAATACCTGCGGAGCTGTACTCGCCAACTCCAAGATCCGAAGGGAACTCGACGCTAACATCTGAAGAGCAACATATGGCACTGAATAAGCTCAAGAAAAAAGTGTATGATCCTCCGAGTAACACAAGTAGGAGAATAAGCTTCTATTACAGAGAGAATAACAGAGGAAATTACAATGAGAGGAACAACAGCAACAGTGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAGGTGATGACAACGCCCTGCAACCATATGTTCCACGAAGAATGCATAGTTCCATGGGTGAAGAGCAATGGCCAATGTCCGGTCTGCAGATTTACCTTGTTCGAGCGAACAGCTCGAAGAGAAGCTTCGTCCAACAACACTGTATCAACTGCGCCGCCTAGTAGCCTAGTCGACGACGAGCTCATGTACTTGATACGAGCCATGGAAGATGCTCTGATGCTGCGAAATCTGGCCGGATTTTGGGGAGCATGAAGCGCCATCTCGATTTTTCACCTGAAATCAACATGCAAAATGCTCTAAATTTGAAGGAATGATGAAATCCGATCTCTAAAAATTAATGTTGTGCTTTGAACATGAAATAATTCGACGAGAGATCTCATTGTGCCAATAAAAGCATCCTCTCGCCGATGACATATTCGATCGATTGAGTTTCTTCATGATCTTGCGCTATAATTCTTCATCCCCATTGCTGAATCAAATGTGATGTTCTTGAAATTCTTGGCGCCAAACTTATATATTTCTGTAGATGACCTGCAATTTTTTTTTTTTTTTTTAAAAAAACGAAATTGAACCGTTTCTTCACATCAAAGCTTTCTAATATCTTACTAGTGGAATTTTTGTACGATGACTCCTTTTGAGTTTGAACGCCTATTTTTCGCTTTTTACTTATGTTATTATTGAAT

mRNA sequence

ATGAGCAGACGTTCTACTAATTTTCCTCCAGGAATTTGGCAGGCCAGAAGGGAATATGATCGCCAGAGATATAACAGAAATGATTCAAACCATACGAACTCTACATCGTCGCATCCTCATTCATCTCAGGTCAGCGGGTTAACAATTCTTTTGTTTTGCAACAAACTGTATTCCAATCCCACGGCCACCATCAAAGCTTATGTTGTTATTCTACGCCATGATTTTCAGGCCATAAAACAGAACTTAGAGATAGATCCAGTTCCATGGCGACCTGCTGGGGAGACAATGATTGCAAGAATATTGGACATCGGGAGGCAAACCAAAGAAGTTGGTATTGCAGATTCACTTCAAGAACCACCTGCAATAGTCAGAATCATCACACACTCCAGACTGAGACAGCGTGAGGCCAGTCCAATACCTGCGGAGCTGTACTCGCCAACTCCAAGATCCGAAGGGAACTCGACGCTAACATCTGAAGAGCAACATATGGCACTGAATAAGCTCAAGAAAAAAGTGTATGATCCTCCGAGTAACACAAGTAGGAGAATAAGCTTCTATTACAGAGAGAATAACAGAGGAAATTACAATGAGAGGAACAACAGCAACAGTGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAGGTGATGACAACGCCCTGCAACCATATGTTCCACGAAGAATGCATAGTTCCATGGGTGAAGAGCAATGGCCAATGTCCGGTCTGCAGATTTACCTTGTTCGAGCGAACAGCTCGAAGAGAAGCTTCGTCCAACAACACTGTATCAACTGCGCCGCCTAGTAGCCTAGTCGACGACGAGCTCATGTACTTGATACGAGCCATGGAAGATGCTCTGATGCTGCGAAATCTGGCCGGATTTTGGGGAGCATGAAGCGCCATCTCGATTTTTCACCTGAAATCAACATGCAAAATGCTCTAAATTTGAAGGAATGATGAAATCCGATCTCTAAAAATTAATGTTGTGCTTTGAACATGAAATAATTCGACGAGAGATCTCATTGTGCCAATAAAAGCATCCTCTCGCCGATGACATATTCGATCGATTGAGTTTCTTCATGATCTTGCGCTATAATTCTTCATCCCCATTGCTGAATCAAATGTGATGTTCTTGAAATTCTTGGCGCCAAACTTATATATTTCTGTAGATGACCTGCAATTTTTTTTTTTTTTTTTAAAAAAACGAAATTGAACCGTTTCTTCACATCAAAGCTTTCTAATATCTTACTAGTGGAATTTTTGTACGATGACTCCTTTTGAGTTTGAACGCCTATTTTTCGCTTTTTACTTATGTTATTATTGAAT

Coding sequence (CDS)

ATGAGCAGACGTTCTACTAATTTTCCTCCAGGAATTTGGCAGGCCAGAAGGGAATATGATCGCCAGAGATATAACAGAAATGATTCAAACCATACGAACTCTACATCGTCGCATCCTCATTCATCTCAGGTCAGCGGGTTAACAATTCTTTTGTTTTGCAACAAACTGTATTCCAATCCCACGGCCACCATCAAAGCTTATGTTGTTATTCTACGCCATGATTTTCAGGCCATAAAACAGAACTTAGAGATAGATCCAGTTCCATGGCGACCTGCTGGGGAGACAATGATTGCAAGAATATTGGACATCGGGAGGCAAACCAAAGAAGTTGGTATTGCAGATTCACTTCAAGAACCACCTGCAATAGTCAGAATCATCACACACTCCAGACTGAGACAGCGTGAGGCCAGTCCAATACCTGCGGAGCTGTACTCGCCAACTCCAAGATCCGAAGGGAACTCGACGCTAACATCTGAAGAGCAACATATGGCACTGAATAAGCTCAAGAAAAAAGTGTATGATCCTCCGAGTAACACAAGTAGGAGAATAAGCTTCTATTACAGAGAGAATAACAGAGGAAATTACAATGAGAGGAACAACAGCAACAGTGATGAAGAAGGCAAGAGATGCGCCGTTTGCCTTGAAGATTTCCAGCCAAAAGAGGAGGTGATGACAACGCCCTGCAACCATATGTTCCACGAAGAATGCATAGTTCCATGGGTGAAGAGCAATGGCCAATGTCCGGTCTGCAGATTTACCTTGTTCGAGCGAACAGCTCGAAGAGAAGCTTCGTCCAACAACACTGTATCAACTGCGCCGCCTAGTAGCCTAGTCGACGACGAGCTCATGTACTTGATACGAGCCATGGAAGATGCTCTGATGCTGCGAAATCTGGCCGGATTTTGGGGAGCATGA

Protein sequence

MSRRSTNFPPGIWQARREYDRQRYNRNDSNHTNSTSSHPHSSQVSGLTILLFCNKLYSNPTATIKAYVVILRHDFQAIKQNLEIDPVPWRPAGETMIARILDIGRQTKEVGIADSLQEPPAIVRIITHSRLRQREASPIPAELYSPTPRSEGNSTLTSEEQHMALNKLKKKVYDPPSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASSNNTVSTAPPSSLVDDELMYLIRAMEDALMLRNLAGFWGA

Homology

[Close]

BLAST of Lsi11G017130 vs. Swiss-Prot
Match: RN115_HUMAN (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens GN=RNF115 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 4.8e-11
Identity = 28/59 (47.46%), Postives = 37/59 (62.71%), Query Frame = 1


Query: 207 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASS 266
           G  C VC ED+  +EEV   PCNH FH  CIVPW++ +  CPVCR +L    + R++ S
Sbjct: 225 GLECPVCKEDYTVEEEVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQSQS 283

[Close]

BLAST of Lsi11G017130 vs. Swiss-Prot
Match: RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 6.2e-11
Identity = 30/63 (47.62%), Postives = 37/63 (58.73%), Query Frame = 1


Query: 210 CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASSNNTV 269
           CAVC E F+ + E    PC H+FH++CIVPW+     CPVCRF L     RR  S+NN  
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNNEE 258

Query: 270 STA 273
             A
Sbjct: 259 DNA 259

[Close]

BLAST of Lsi11G017130 vs. Swiss-Prot
Match: YKW3_SCHPO (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAP32A8.03c PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.1e-11
Identity = 28/73 (38.36%), Postives = 46/73 (63.01%), Query Frame = 1


Query: 204 DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREA 263
           DEEG+ C +C+E F+  ++V+  PC H FHE CI PW++ NG C +CR  +   + +R  
Sbjct: 391 DEEGE-CTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPVDPNSQQRNN 450

Query: 264 SSNNTVSTAPPSS 277
           +S ++ +   PS+
Sbjct: 451 TSTDSANGHNPSN 462

[Close]

BLAST of Lsi11G017130 vs. Swiss-Prot
Match: RN115_MOUSE (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-10
Identity = 31/69 (44.93%), Postives = 41/69 (59.42%), Query Frame = 1


Query: 207 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTL-----FERTARR 266
           G  C VC ED+  +E+V   PCNH FH  CIVPW++ +  CPVCR +L       +T   
Sbjct: 226 GLECPVCKEDYTVEEKVRQLPCNHFFHSSCIVPWLELHDTCPVCRKSLNGEDSTRQTQSS 285

Query: 267 EASSNNTVS 271
           EAS++N  S
Sbjct: 286 EASASNRFS 294

[Close]

BLAST of Lsi11G017130 vs. Swiss-Prot
Match: RN126_MOUSE (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.4e-10
Identity = 32/79 (40.51%), Postives = 47/79 (59.49%), Query Frame = 1


Query: 207 GKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLF-ERTARRE--- 266
           G  C VC ED+   E V   PCNH+FH+ CIVPW++ +  CPVCR +L  + TA      
Sbjct: 228 GLECPVCKEDYALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCRKSLTGQNTATNPPGL 287

Query: 267 -----ASSNNTVSTAPPSS 277
                +SS+++ S++ PS+
Sbjct: 288 TGVGFSSSSSSSSSSSPSN 306

[Close]

BLAST of Lsi11G017130 vs. TrEMBL
Match: A0A0A0K1V1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G049220 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 9.7e-112
Identity = 220/307 (71.66%), Postives = 239/307 (77.85%), Query Frame = 1


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNHTNSTSSHPHSSQVSGLTILLFCNKLYSNP 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN+SN T   +S P  +Q                 
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRT---NSTPSHTQ----------------- 60

Query: 61  TATIKAYVVILRHDFQAIKQNLEIDPVPWRPAGETMIARILDIGRQTKEVGIADSLQEPP 120
                         FQ      +IDPVPWRPAGET+I RILDIG +          +EPP
Sbjct: 61  -------------SFQ--NSYRQIDPVPWRPAGETVITRILDIGSRP---------EEPP 120

Query: 121 AIVRIITHSRLRQREASPIPAELYSPTPRSEGNSTLTSEEQHMALNKLKKKVYDPPSNTS 180
           AIVRIITHSR RQ EASPIPAELYSPTPRSEGNSTLTSEEQ+MALNKLKKKVYDPPSNTS
Sbjct: 121 AIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTS 180

Query: 181 RRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPW 240
           RRISFYYRENNR N NER+NSN DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHE+CIVPW
Sbjct: 181 RRISFYYRENNRVNSNERHNSNGDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPW 240

Query: 241 VKSNGQCPVCRFTLFERTARREASS---NNTVSTAPPSSLVDDELMYLIRAMEDALMLRN 300
           VKS+GQCPVCRFTLFERT RR ++S   NN+VS APPSSLVD+EL+YLIRAME+ALMLRN
Sbjct: 241 VKSHGQCPVCRFTLFERTERRGSASSNFNNSVSIAPPSSLVDEELVYLIRAMEEALMLRN 263

Query: 301 LAGFWGA 305
           +A FWGA
Sbjct: 301 VAEFWGA 263

[Close]

BLAST of Lsi11G017130 vs. TrEMBL
Match: A0A061ECA4_THECC (RING/U-box superfamily protein, putative OS=Theobroma cacao GN=TCM_008926 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.1e-37
Identity = 80/153 (52.29%), Postives = 105/153 (68.63%), Query Frame = 1


Query: 147 TPRSEGNSTLTSEEQHMALNKLKKKVYDP-PSNTSRRISFYYRENNRGNYNERNNSNSDE 206
           +P  + +S    +EQ   L+KLKK++Y+P P   +RR+S YYR+ NR N         DE
Sbjct: 122 SPAQQEDSRSARDEQSNVLSKLKKEIYNPIPKQMTRRLSMYYRDQNRSNLYNEKEREKDE 181

Query: 207 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREAS- 266
           +GKRCA+CLEDF+P+E+VM TPC+HMFHEECIVPWVKS+GQCPVCRF L ER  +  +S 
Sbjct: 182 DGKRCAICLEDFEPREQVMLTPCDHMFHEECIVPWVKSHGQCPVCRFVLSERIRQNASSI 241

Query: 267 SNNTVSTAPPSSLVDDELMYLIRAMEDALMLRN 298
           SNN  S    + L   EL+ +IRAME+A +  N
Sbjct: 242 SNNNASNVAATDLFAGELISIIRAMEEAFLWGN 274

[Close]

BLAST of Lsi11G017130 vs. TrEMBL
Match: V4U2F9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016257mg PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.3e-35
Identity = 87/194 (44.85%), Postives = 115/194 (59.28%), Query Frame = 1


Query: 112 IADSLQEPPAIVRIITHSRLRQREASPIPAELYSPTP-----RSEGNSTLTSEEQHMALN 171
           +A  L +  + VRI     L  RE  P P ++   +P               +EQ+  L+
Sbjct: 55  LAGVLGQSQSSVRIPILGNLMAREVRPRPRQIIQGSPPRFLVNRPNILPAQEDEQNAVLS 114

Query: 172 KLKKKVYDP-PSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMT 231
           KLKK+VY+P P N ++R+S YYR+     Y E      +E+  RCAVCLEDF+PKE+VM 
Sbjct: 115 KLKKEVYNPAPMNLAKRLSLYYRDQATNVYKEM-QKQKEEDAMRCAVCLEDFEPKEKVML 174

Query: 232 TPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASSNNTVSTAPPSSLVDDELMYL 291
           TPCNHMFHE+CIVPWVKSN QCPVCRF+L E      AS NN  + A    +   EL+ +
Sbjct: 175 TPCNHMFHEDCIVPWVKSNAQCPVCRFSLGENKRESRASDNNIANAAVSDEVFAMELVSV 234

Query: 292 IRAMEDALMLRNLA 300
           IRAME++L+  N A
Sbjct: 235 IRAMEESLLWNNAA 247

[Close]

BLAST of Lsi11G017130 vs. TrEMBL
Match: V4UCS4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016257mg PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.3e-35
Identity = 87/194 (44.85%), Postives = 115/194 (59.28%), Query Frame = 1


Query: 112 IADSLQEPPAIVRIITHSRLRQREASPIPAELYSPTP-----RSEGNSTLTSEEQHMALN 171
           +A  L +  + VRI     L  RE  P P ++   +P               +EQ+  L+
Sbjct: 77  LAGVLGQSQSSVRIPILGNLMAREVRPRPRQIIQGSPPRFLVNRPNILPAQEDEQNAVLS 136

Query: 172 KLKKKVYDP-PSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMT 231
           KLKK+VY+P P N ++R+S YYR+     Y E      +E+  RCAVCLEDF+PKE+VM 
Sbjct: 137 KLKKEVYNPAPMNLAKRLSLYYRDQATNVYKEM-QKQKEEDAMRCAVCLEDFEPKEKVML 196

Query: 232 TPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASSNNTVSTAPPSSLVDDELMYL 291
           TPCNHMFHE+CIVPWVKSN QCPVCRF+L E      AS NN  + A    +   EL+ +
Sbjct: 197 TPCNHMFHEDCIVPWVKSNAQCPVCRFSLGENKRESRASDNNIANAAVSDEVFAMELVSV 256

Query: 292 IRAMEDALMLRNLA 300
           IRAME++L+  N A
Sbjct: 257 IRAMEESLLWNNAA 269

[Close]

BLAST of Lsi11G017130 vs. TrEMBL
Match: A0A0K9REK9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_080190 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.6e-34
Identity = 91/220 (41.36%), Postives = 129/220 (58.64%), Query Frame = 1


Query: 86  PVPWRPAGETMIARILDIGRQTKEVGIADSLQE--PPAIVRIITHSRLRQREASPIPAEL 145
           P  +RP  ET + R    G  +   G  D +    P +  RII   R    E++ +P E 
Sbjct: 33  PAGYRPFEETPVIR--PFGLTSNNTGHGDGMNPRFPQSSQRIIP--RYTSNESTHVPTE- 92

Query: 146 YSPTPRSEGNSTLTSEEQHMALNKLKKKVYDP-PSNTSRRISFYYRENNRGNYNERNNSN 205
                 ++ +S LT EEQ + L KLKK++Y+P P   +RR+S YYR+ NR   ++    +
Sbjct: 93  ------TDDDSKLTKEEQVIVLKKLKKEIYNPMPKILARRVSLYYRDVNRDITSKSMEKH 152

Query: 206 SDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTAR-- 265
            DE+GK CAVCLEDF+ +EEVM TPC+HMFHEEC+VPWVK++G CPVCR T +ER  R  
Sbjct: 153 KDEDGKSCAVCLEDFEAREEVMVTPCSHMFHEECVVPWVKNHGDCPVCRATFYERRERLI 212

Query: 266 --REASSNNTVSTAPPSSLVDDELMYLIRAMEDALMLRNL 299
             +  + NN     P  +L  DEL+ L +++++A   R L
Sbjct: 213 RGQPFNDNNRGVRVP--ALFSDELLSLFQSLDEAFPWRFL 239

[Close]

BLAST of Lsi11G017130 vs. TAIR10
Match: AT3G58720.2 (AT3G58720.2 RING/U-box superfamily protein)

HSP 1 Score: 133.7 bits (335), Expect = 2.0e-31
Identity = 77/192 (40.10%), Postives = 113/192 (58.85%), Query Frame = 1


Query: 119 PPAIVR----IITHSRLRQREASPIPAELYSPTPRSEGNSTLTSE--EQHMALNKLKKKV 178
           PP+  R    I   + LR++    +   +++ + R E +S  +S+  E+   L  L K+ 
Sbjct: 71  PPSHTRHQIPIRNSNNLRRQHLEALRQAVHNQSTRPESSSRSSSQTNEEDEILKHLTKET 130

Query: 179 YDP-PSNTS-RRISFYYRENNRGNYNERNNSN--SDEEGKRCAVCLEDFQPKEEVMTTPC 238
           Y+P P +T  R +S YYR  N G+ + RN  N   +E+ KRC+VCLEDF+PKE VM TPC
Sbjct: 131 YNPVPKSTLLRSLSLYYRNKNPGSESSRNTQNLSGEEDDKRCSVCLEDFEPKETVMLTPC 190

Query: 239 NHMFHEECIVPWVKSNGQCPVCRFTLFERTARRE---ASSNNTVSTAPPSSLVDDELMYL 298
            HMFHEECIVPW+K+ GQCPVCRF + + T +     A+ +N       + L   EL+ +
Sbjct: 191 KHMFHEECIVPWLKTKGQCPVCRFVILKPTKQDSSPPANGSNLTGDMTTNDLFTLELLSV 250

[Close]

BLAST of Lsi11G017130 vs. TAIR10
Match: AT5G59550.1 (AT5G59550.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 69.7 bits (169), Expect = 3.5e-12
Identity = 30/63 (47.62%), Postives = 37/63 (58.73%), Query Frame = 1


Query: 210 CAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASSNNTV 269
           CAVC E F+ + E    PC H+FH++CIVPW+     CPVCRF L     RR  S+NN  
Sbjct: 199 CAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEPNRR--SNNNEE 258

Query: 270 STA 273
             A
Sbjct: 259 DNA 259

[Close]

BLAST of Lsi11G017130 vs. TAIR10
Match: AT2G44330.1 (AT2G44330.1 RING/U-box superfamily protein)

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-11
Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = 1


Query: 199 NNSNSDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTL 255
           ++++SD+    CA+C EDF   E     PCNH++H +CI+PW+ S+  CP+CR  L
Sbjct: 85  SSASSDDSALPCAICREDFVVGESARRLPCNHLYHNDCIIPWLTSHNSCPLCRVEL 140

[Close]

BLAST of Lsi11G017130 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-11
Identity = 22/49 (44.90%), Postives = 36/49 (73.47%), Query Frame = 1


Query: 206 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTL 255
           E  +CAVC+++F+   +V   PC H+FH++C++PW++ +  CPVCRF L
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of Lsi11G017130 vs. TAIR10
Match: AT1G55530.1 (AT1G55530.1 RING/U-box superfamily protein)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-11
Identity = 29/72 (40.28%), Postives = 44/72 (61.11%), Query Frame = 1


Query: 205 EEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREA- 264
           EE  +C+VCL+DF+   E    PC H FH +C++PW++ +  CPVCR+ L    A+ ++ 
Sbjct: 218 EETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSV 277

Query: 265 ---SSNNTVSTA 273
              S NN  S+A
Sbjct: 278 TTTSDNNGSSSA 289

[Close]

BLAST of Lsi11G017130 vs. NCBI nr
Match: gi|659110554|ref|XP_008455285.1| (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo])

HSP 1 Score: 417.9 bits (1073), Expect = 1.5e-113
Identity = 226/307 (73.62%), Postives = 238/307 (77.52%), Query Frame = 1


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNHTNSTSSHPHSSQVSGLTILLFCNKLYSNP 60
           MSRRSTNFPPGIWQARREYDRQRYNRN+SN                        ++ S P
Sbjct: 1   MSRRSTNFPPGIWQARREYDRQRYNRNESN------------------------RVNSTP 60

Query: 61  TATIKAYVVILRHDFQAIKQNLEIDPVPWRPAGETMIARILDIGRQTKEVGIADSLQEPP 120
           + T           FQ      EIDPVPWRPAGETMI RILDIG            +EPP
Sbjct: 61  SHT---------QSFQ--NSYREIDPVPWRPAGETMITRILDIGSLP---------EEPP 120

Query: 121 AIVRIITHSRLRQREASPIPAELYSPTPRSEGNSTLTSEEQHMALNKLKKKVYDPPSNTS 180
           AIVRIITHSR RQ EASPIPAELYSPTPRSEGNSTLTSEEQ+MALNKLKKKVYDPPSNTS
Sbjct: 121 AIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTS 180

Query: 181 RRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPW 240
           RRI+FYYRENNR NYNER+N+NSDEEGK CAVCLEDFQPKEEVMTTPCNHMFHE+CIVPW
Sbjct: 181 RRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPW 240

Query: 241 VKSNGQCPVCRFTLFE-RTARREASS--NNTVSTAPPSSLVDDELMYLIRAMEDALMLRN 300
           VKSNGQCPVCRFTLFE RT RR ASS  NN VS APPSSLVDDELMYLIRAME+ALMLRN
Sbjct: 241 VKSNGQCPVCRFTLFERRTERRGASSNFNNNVSIAPPSSLVDDELMYLIRAMEEALMLRN 263

Query: 301 LAGFWGA 305
           +A FWGA
Sbjct: 301 VAEFWGA 263

[Close]

BLAST of Lsi11G017130 vs. NCBI nr
Match: gi|449438121|ref|XP_004136838.1| (PREDICTED: uncharacterized protein LOC101210416 [Cucumis sativus])

HSP 1 Score: 411.4 bits (1056), Expect = 1.4e-111
Identity = 220/307 (71.66%), Postives = 239/307 (77.85%), Query Frame = 1


Query: 1   MSRRSTNFPPGIWQARREYDRQRYNRNDSNHTNSTSSHPHSSQVSGLTILLFCNKLYSNP 60
           MSRRSTNFPPGIWQARRE+DRQRYNRN+SN T   +S P  +Q                 
Sbjct: 1   MSRRSTNFPPGIWQARREHDRQRYNRNESNRT---NSTPSHTQ----------------- 60

Query: 61  TATIKAYVVILRHDFQAIKQNLEIDPVPWRPAGETMIARILDIGRQTKEVGIADSLQEPP 120
                         FQ      +IDPVPWRPAGET+I RILDIG +          +EPP
Sbjct: 61  -------------SFQ--NSYRQIDPVPWRPAGETVITRILDIGSRP---------EEPP 120

Query: 121 AIVRIITHSRLRQREASPIPAELYSPTPRSEGNSTLTSEEQHMALNKLKKKVYDPPSNTS 180
           AIVRIITHSR RQ EASPIPAELYSPTPRSEGNSTLTSEEQ+MALNKLKKKVYDPPSNTS
Sbjct: 121 AIVRIITHSRPRQLEASPIPAELYSPTPRSEGNSTLTSEEQNMALNKLKKKVYDPPSNTS 180

Query: 181 RRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPW 240
           RRISFYYRENNR N NER+NSN DEEGKRCAVCLEDFQPKEEVMTTPCNHMFHE+CIVPW
Sbjct: 181 RRISFYYRENNRVNSNERHNSNGDEEGKRCAVCLEDFQPKEEVMTTPCNHMFHEQCIVPW 240

Query: 241 VKSNGQCPVCRFTLFERTARREASS---NNTVSTAPPSSLVDDELMYLIRAMEDALMLRN 300
           VKS+GQCPVCRFTLFERT RR ++S   NN+VS APPSSLVD+EL+YLIRAME+ALMLRN
Sbjct: 241 VKSHGQCPVCRFTLFERTERRGSASSNFNNSVSIAPPSSLVDEELVYLIRAMEEALMLRN 263

Query: 301 LAGFWGA 305
           +A FWGA
Sbjct: 301 VAEFWGA 263

[Close]

BLAST of Lsi11G017130 vs. NCBI nr
Match: gi|659110556|ref|XP_008455287.1| (PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X2 [Cucumis melo])

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-90
Identity = 177/208 (85.10%), Postives = 189/208 (90.87%), Query Frame = 1


Query: 102 DIGRQ-TKEVGIADSL-QEPPAIVRIITHSRLRQREASPIPAELYSPTPRSEGNSTLTSE 161
           ++G+Q TK   + D L +EPPAIVRIITHSR RQ EASPIPAELYSPTPRSEGNSTLTSE
Sbjct: 35  NLGKQITKCQILGDCLPEEPPAIVRIITHSRPRQIEASPIPAELYSPTPRSEGNSTLTSE 94

Query: 162 EQHMALNKLKKKVYDPPSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQP 221
           EQ+MALNKLKKKVYDPPSNTSRRI+FYYRENNR NYNER+N+NSDEEGK CAVCLEDFQP
Sbjct: 95  EQNMALNKLKKKVYDPPSNTSRRINFYYRENNRVNYNERHNNNSDEEGKGCAVCLEDFQP 154

Query: 222 KEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFE-RTARREASS--NNTVSTAPPSS 281
           KEEVMTTPCNHMFHE+CIVPWVKSNGQCPVCRFTLFE RT RR ASS  NN VS APPSS
Sbjct: 155 KEEVMTTPCNHMFHEQCIVPWVKSNGQCPVCRFTLFERRTERRGASSNFNNNVSIAPPSS 214

Query: 282 LVDDELMYLIRAMEDALMLRNLAGFWGA 305
           LVDDELMYLIRAME+ALMLRN+A FWGA
Sbjct: 215 LVDDELMYLIRAMEEALMLRNVAEFWGA 242

[Close]

BLAST of Lsi11G017130 vs. NCBI nr
Match: gi|590692552|ref|XP_007044087.1| (RING/U-box superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 164.5 bits (415), Expect = 3.0e-37
Identity = 80/153 (52.29%), Postives = 105/153 (68.63%), Query Frame = 1


Query: 147 TPRSEGNSTLTSEEQHMALNKLKKKVYDP-PSNTSRRISFYYRENNRGNYNERNNSNSDE 206
           +P  + +S    +EQ   L+KLKK++Y+P P   +RR+S YYR+ NR N         DE
Sbjct: 122 SPAQQEDSRSARDEQSNVLSKLKKEIYNPIPKQMTRRLSMYYRDQNRSNLYNEKEREKDE 181

Query: 207 EGKRCAVCLEDFQPKEEVMTTPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREAS- 266
           +GKRCA+CLEDF+P+E+VM TPC+HMFHEECIVPWVKS+GQCPVCRF L ER  +  +S 
Sbjct: 182 DGKRCAICLEDFEPREQVMLTPCDHMFHEECIVPWVKSHGQCPVCRFVLSERIRQNASSI 241

Query: 267 SNNTVSTAPPSSLVDDELMYLIRAMEDALMLRN 298
           SNN  S    + L   EL+ +IRAME+A +  N
Sbjct: 242 SNNNASNVAATDLFAGELISIIRAMEEAFLWGN 274

[Close]

BLAST of Lsi11G017130 vs. NCBI nr
Match: gi|567909045|ref|XP_006446836.1| (hypothetical protein CICLE_v10016257mg [Citrus clementina])

HSP 1 Score: 157.1 bits (396), Expect = 4.7e-35
Identity = 87/194 (44.85%), Postives = 115/194 (59.28%), Query Frame = 1


Query: 112 IADSLQEPPAIVRIITHSRLRQREASPIPAELYSPTP-----RSEGNSTLTSEEQHMALN 171
           +A  L +  + VRI     L  RE  P P ++   +P               +EQ+  L+
Sbjct: 77  LAGVLGQSQSSVRIPILGNLMAREVRPRPRQIIQGSPPRFLVNRPNILPAQEDEQNAVLS 136

Query: 172 KLKKKVYDP-PSNTSRRISFYYRENNRGNYNERNNSNSDEEGKRCAVCLEDFQPKEEVMT 231
           KLKK+VY+P P N ++R+S YYR+     Y E      +E+  RCAVCLEDF+PKE+VM 
Sbjct: 137 KLKKEVYNPAPMNLAKRLSLYYRDQATNVYKEM-QKQKEEDAMRCAVCLEDFEPKEKVML 196

Query: 232 TPCNHMFHEECIVPWVKSNGQCPVCRFTLFERTARREASSNNTVSTAPPSSLVDDELMYL 291
           TPCNHMFHE+CIVPWVKSN QCPVCRF+L E      AS NN  + A    +   EL+ +
Sbjct: 197 TPCNHMFHEDCIVPWVKSNAQCPVCRFSLGENKRESRASDNNIANAAVSDEVFAMELVSV 256

Query: 292 IRAMEDALMLRNLA 300
           IRAME++L+  N A
Sbjct: 257 IRAMEESLLWNNAA 269

The following BLAST results are available for this feature:

BLAST of Lsi11G017130 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RN115_HUMAN	4.8e-11	47.46	E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens GN=RNF115 PE=1 SV=2	[more]
RDUF2_ARATH	6.2e-11	47.62	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1	[more]
YKW3_SCHPO	8.1e-11	38.36	Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra...	[more]
RN115_MOUSE	1.1e-10	44.93	E3 ubiquitin-protein ligase RNF115 OS=Mus musculus GN=Rnf115 PE=1 SV=1	[more]
RN126_MOUSE	2.4e-10	40.51	E3 ubiquitin-protein ligase RNF126 OS=Mus musculus GN=Rnf126 PE=1 SV=1	[more]

BLAST of Lsi11G017130 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K1V1_CUCSA	9.7e-112	71.66	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G049220 PE=4 SV=1	[more]
A0A061ECA4_THECC	2.1e-37	52.29	RING/U-box superfamily protein, putative OS=Theobroma cacao GN=TCM_008926 PE=4 S...	[more]
V4U2F9_9ROSI	3.3e-35	44.85	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016257mg PE=4 SV=1	[more]
V4UCS4_9ROSI	3.3e-35	44.85	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016257mg PE=4 SV=1	[more]
A0A0K9REK9_SPIOL	3.6e-34	41.36	Uncharacterized protein OS=Spinacia oleracea GN=SOVF_080190 PE=4 SV=1	[more]

BLAST of Lsi11G017130 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G58720.2	2.0e-31	40.10	RING/U-box superfamily protein	[more]
AT5G59550.1	3.5e-12	47.62	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G44330.1	1.3e-11	42.86	RING/U-box superfamily protein	[more]
AT3G19950.1	1.7e-11	44.90	RING/U-box superfamily protein	[more]
AT1G55530.1	1.7e-11	40.28	RING/U-box superfamily protein	[more]

BLAST of Lsi11G017130 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659110554\|ref\|XP_008455285.1\|	1.5e-113	73.62	PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X1 [Cucumis melo]	[more]
gi\|449438121\|ref\|XP_004136838.1\|	1.4e-111	71.66	PREDICTED: uncharacterized protein LOC101210416 [Cucumis sativus]	[more]
gi\|659110556\|ref\|XP_008455287.1\|	3.0e-90	85.10	PREDICTED: E3 ubiquitin-protein ligase RNF12 isoform X2 [Cucumis melo]	[more]
gi\|590692552\|ref\|XP_007044087.1\|	3.0e-37	52.29	RING/U-box superfamily protein, putative [Theobroma cacao]	[more]
gi\|567909045\|ref\|XP_006446836.1\|	4.7e-35	44.85	hypothetical protein CICLE_v10016257mg [Citrus clementina]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044267	cellular protein metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G017130.1	Lsi11G017130.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 209..251 score: 2.1
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 210..250 score: 1.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 210..251 score: 13
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 205..254 score: 1.6
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 205..296 score: 8.5
None	No IPR available	PANTHER	PTHR22763:SF3	E3 UBIQUITIN-PROTEIN LIGASE RNF115	coord: 205..296 score: 8.5
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 202..259 score: 1.46

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi11G017130	Csa7G049220	Cucumber (Chinese Long) v2	culsiB500
Lsi11G017130	CSPI07G04880	Wild cucumber (PI 183967)	cpilsiB512
Lsi11G017130	Cucsa.195670	Cucumber (Gy14) v1	cgylsiB332
Lsi11G017130	CmaCh11G019090	Cucurbita maxima (Rimu)	cmalsiB101
Lsi11G017130	CmaCh19G010010	Cucurbita maxima (Rimu)	cmalsiB473
Lsi11G017130	CmoCh11G019890	Cucurbita moschata (Rifu)	cmolsiB087
Lsi11G017130	Cla002238	Watermelon (97103) v1	lsiwmB095
Lsi11G017130	MELO3C018712	Melon (DHL92) v3.5.1	lsimeB092
Lsi11G017130	Cp4.1LG04g02740	Cucurbita pepo (Zucchini)	cpelsiB531
Lsi11G017130	CsGy7G004420	Cucumber (Gy14) v2	cgyblsiB469
Lsi11G017130	Carg19150	Silver-seed gourd	carlsiB660
Lsi11G017130	CsaV3_7G005800	Cucumber (Chinese Long) v3	cuclsiB534

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G017130	Watermelon (97103) v2	lsiwmbB100
Lsi11G017130	Wax gourd	lsiwgoB119
Lsi11G017130	Cucurbita maxima (Rimu)	cmalsiB474
Lsi11G017130	Cucurbita moschata (Rifu)	cmolsiB458
Lsi11G017130	Cucurbita moschata (Rifu)	cmolsiB459
Lsi11G017130	Cucurbita pepo (Zucchini)	cpelsiB200
Lsi11G017130	Cucurbita pepo (Zucchini)	cpelsiB201
Lsi11G017130	Watermelon (Charleston Gray)	lsiwcgB113
Lsi11G017130	Melon (DHL92) v3.6.1	lsimedB098
Lsi11G017130	Silver-seed gourd	carlsiB796