Lsi11G006510 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G006510
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	chr11 : 6865538 .. 6865792 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATGACTTGATAGTATGTGAAAATGCATATAAGATAGTGAAGGAAGCATTTGAGGAAGGGATTAAGTTATTCTCACAAGGAGATTACAAAGGAATGTTGAATTCTGAGAGGATTACACCAAGAGCACAAGCAAGTTGTGATGAGATTTTCAGTACACCACCAGCTAAACAAAGCCCATTGTTGGAAAGGAATAGGGAGATGAGGATTTTGATTGCTATGGCTATAGTTTCTGGTTCAAGTATTCCATGA

mRNA sequence

ATGAAGAATGACTTGATAGTATGTGAAAATGCATATAAGATAGTGAAGGAAGCATTTGAGGAAGGGATTAAGTTATTCTCACAAGGAGATTACAAAGGAATGTTGAATTCTGAGAGGATTACACCAAGAGCACAAGCAAGTTGTGATGAGATTTTCAGTACACCACCAGCTAAACAAAGCCCATTGTTGGAAAGGAATAGGGAGATGAGGATTTTGATTGCTATGGCTATAGTTTCTGGTTCAAGTATTCCATGA

Coding sequence (CDS)

ATGAAGAATGACTTGATAGTATGTGAAAATGCATATAAGATAGTGAAGGAAGCATTTGAGGAAGGGATTAAGTTATTCTCACAAGGAGATTACAAAGGAATGTTGAATTCTGAGAGGATTACACCAAGAGCACAAGCAAGTTGTGATGAGATTTTCAGTACACCACCAGCTAAACAAAGCCCATTGTTGGAAAGGAATAGGGAGATGAGGATTTTGATTGCTATGGCTATAGTTTCTGGTTCAAGTATTCCATGA

Protein sequence

MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFSTPPAKQSPLLERNREMRILIAMAIVSGSSIP

Homology

[Close]

BLAST of Lsi11G006510 vs. TrEMBL
Match: A0A0A0LGJ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G034680 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.6e-19
Identity = 56/80 (70.00%), Postives = 61/80 (76.25%), Query Frame = 1


Query: 5   LIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIF-STPPAKQSPLL 64
           L+VCEN Y  V EAF EGI+ FSQ  YK MLN ER  P AQASC  IF +TPP KQ+PL 
Sbjct: 104 LLVCENGYYAVNEAFVEGIRWFSQKYYKEMLNVERRAPSAQASCTSIFTTTPPPKQNPLF 163

Query: 65  ERNREMRILIAMAIVSGSSI 84
           + NREMRILIAMAIVSGSSI
Sbjct: 164 QINREMRILIAMAIVSGSSI 183

[Close]

BLAST of Lsi11G006510 vs. TrEMBL
Match: A0A0A0LM47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G034690 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 3.5e-19
Identity = 55/83 (66.27%), Postives = 61/83 (73.49%), Query Frame = 1


Query: 2   KNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIF-STPPAKQS 61
           K  L+VCEN Y  V EAF EGI+ FSQ  YK MLN ER  P AQASC  IF +TPP KQ+
Sbjct: 74  KKALLVCENGYYAVNEAFVEGIRWFSQKYYKEMLNVERRAPSAQASCTSIFTTTPPPKQN 133

Query: 62  PLLERNREMRILIAMAIVSGSSI 84
           PL + NR+MRILIAMAI SGSSI
Sbjct: 134 PLFQINRDMRILIAMAIASGSSI 156

[Close]

BLAST of Lsi11G006510 vs. TrEMBL
Match: A0A0D2ST66_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G298200 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.9e-17
Identity = 46/82 (56.10%), Postives = 58/82 (70.73%), Query Frame = 1


Query: 2   KNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFSTPPAKQSP 61
           KN L  CENAY++V   F E    F +GDY  ML+SER +PRAQ SC  IFSTPP   +P
Sbjct: 118 KNALTTCENAYRLVTSNFNEATMHFFEGDYGSMLDSERRSPRAQESCITIFSTPPTPPNP 177

Query: 62  LLERNREMRILIAMAIVSGSSI 84
           + +RNR+MRILIAMA+V+G  +
Sbjct: 178 VADRNRQMRILIAMAVVTGMEL 199

[Close]

BLAST of Lsi11G006510 vs. TrEMBL
Match: B9N451_POPTR (Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa GN=POPTR_0005s00890g PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.6e-17
Identity = 44/83 (53.01%), Postives = 58/83 (69.88%), Query Frame = 1


Query: 1   MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFSTPPAKQS 60
           ++N L+ CENA+ IVK +F   ++ F++ DY  M   ER+ PRAQASC+  F+ PP+  +
Sbjct: 96  LRNVLVACENAFGIVKSSFGAALQSFNRKDYDDMFKLERVAPRAQASCETSFTAPPSPPN 155

Query: 61  PLLERNREMRILIAMAIVSGSSI 84
           PL ERNREMRILI MAI SG  I
Sbjct: 156 PLAERNREMRILITMAIASGKEI 178

[Close]

BLAST of Lsi11G006510 vs. TrEMBL
Match: B9RED7_RICCO (Enzyme inhibitor, putative OS=Ricinus communis GN=RCOM_1620790 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.1e-16
Identity = 43/81 (53.09%), Postives = 59/81 (72.84%), Query Frame = 1


Query: 1   MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFSTPPAKQS 60
           ++N L VCEN Y IV ++F++  K F+Q DY+ M+ SERITPRAQ S    FS P    +
Sbjct: 96  LENALNVCENGYSIVVQSFQDASKYFNQKDYRSMIQSERITPRAQGSSSTSFSIPSFPVN 155

Query: 61  PLLERNREMRILIAMAIVSGS 82
           PL++RNR+MRI+ AMA+V+GS
Sbjct: 156 PLVDRNRQMRIITAMALVTGS 176

[Close]

BLAST of Lsi11G006510 vs. TAIR10
Match: AT1G09360.1 (AT1G09360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 49.3 bits (116), Expect = 1.4e-06
Identity = 32/79 (40.51%), Postives = 42/79 (53.16%), Query Frame = 1


Query: 5   LIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFSTPPAKQSPLLE 64
           L  C   YK    +F E   LFS+G+Y+ M  +   T +    C   F  PP K+ PLLE
Sbjct: 92  LKTCHAVYKRETNSFLEIRFLFSKGEYELMDEAILSTAKILEECRGDFLIPPYKE-PLLE 151

Query: 65  RNREMRILIAMAIVSGSSI 84
           + R MRILI M+ VSG  +
Sbjct: 152 KKRVMRILITMSAVSGHMV 169

[Close]

BLAST of Lsi11G006510 vs. NCBI nr
Match: gi|659071554|ref|XP_008460590.1| (PREDICTED: uncharacterized protein LOC103499372 [Cucumis melo])

HSP 1 Score: 128.6 bits (322), Expect = 5.0e-27
Identity = 63/84 (75.00%), Postives = 76/84 (90.48%), Query Frame = 1


Query: 1   MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFSTPPAKQS 60
           +KNDLIVCENA++IVK+AF E ++LFSQ DY+GML++ER TPRAQASCD IFSTPPA+ S
Sbjct: 73  LKNDLIVCENAFRIVKQAFGEALRLFSQRDYRGMLSAERPTPRAQASCDVIFSTPPARHS 132

Query: 61  PLL-ERNREMRILIAMAIVSGSSI 84
           P++ ERNREMRILIAMAI SGS+I
Sbjct: 133 PIMDERNREMRILIAMAITSGSNI 156

[Close]

BLAST of Lsi11G006510 vs. NCBI nr
Match: gi|659071542|ref|XP_008460509.1| (PREDICTED: uncharacterized protein LOC103499305 [Cucumis melo])

HSP 1 Score: 125.6 bits (314), Expect = 4.2e-26
Identity = 65/84 (77.38%), Postives = 72/84 (85.71%), Query Frame = 1


Query: 1   MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFS-TPPAKQ 60
           +KN LIVCENAYK+VK+AF EGI+ F Q DYKGMLN+ERI PRAQASC  IFS TPP KQ
Sbjct: 73  LKNALIVCENAYKVVKDAFGEGIQSFVQRDYKGMLNAERIAPRAQASCTSIFSTTPPPKQ 132

Query: 61  SPLLERNREMRILIAMAIVSGSSI 84
           +PL + NREMRILIAMAIVSGSSI
Sbjct: 133 NPLSQINREMRILIAMAIVSGSSI 156

[Close]

BLAST of Lsi11G006510 vs. NCBI nr
Match: gi|659071552|ref|XP_008460578.1| (PREDICTED: uncharacterized protein LOC103499366 [Cucumis melo])

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-25
Identity = 63/84 (75.00%), Postives = 71/84 (84.52%), Query Frame = 1


Query: 1   MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFS-TPPAKQ 60
           +KN LIVCENAYK+VK+AF EGI  F+Q DY+GMLN+ERI PR QASC  IFS TPP KQ
Sbjct: 59  LKNALIVCENAYKVVKDAFGEGIHSFAQRDYRGMLNAERIAPRVQASCTSIFSTTPPPKQ 118

Query: 61  SPLLERNREMRILIAMAIVSGSSI 84
           +PL + NREMRILIAMAIVSGSSI
Sbjct: 119 NPLSQINREMRILIAMAIVSGSSI 142

[Close]

BLAST of Lsi11G006510 vs. NCBI nr
Match: gi|659071548|ref|XP_008460537.1| (PREDICTED: uncharacterized protein LOC103499331 [Cucumis melo])

HSP 1 Score: 114.4 bits (285), Expect = 9.7e-23
Identity = 58/83 (69.88%), Postives = 69/83 (83.13%), Query Frame = 1


Query: 2   KNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFST-PPAKQS 61
           +N LIVCEN Y +V E F EGI+LFS+ +YKGMLN+E+I PRAQASC  IFST PP +Q+
Sbjct: 74  RNRLIVCENGYYVVNETFLEGIRLFSERNYKGMLNTEKIAPRAQASCISIFSTIPPPEQN 133

Query: 62  PLLERNREMRILIAMAIVSGSSI 84
           PL++ NR MRILIAMAIVSGSSI
Sbjct: 134 PLVDINRRMRILIAMAIVSGSSI 156

[Close]

BLAST of Lsi11G006510 vs. NCBI nr
Match: gi|659071550|ref|XP_008460566.1| (PREDICTED: uncharacterized protein LOC103499358 [Cucumis melo])

HSP 1 Score: 111.3 bits (277), Expect = 8.2e-22
Identity = 55/76 (72.37%), Postives = 65/76 (85.53%), Query Frame = 1


Query: 1   MKNDLIVCENAYKIVKEAFEEGIKLFSQGDYKGMLNSERITPRAQASCDEIFS-TPPAKQ 60
           +KN LIVCENAYK+VK++F EGI+ F+Q DY+GMLN+ERI PRAQASC  IFS TPP KQ
Sbjct: 73  LKNALIVCENAYKVVKDSFGEGIQSFAQRDYRGMLNAERIAPRAQASCTSIFSTTPPPKQ 132

Query: 61  SPLLERNREMRILIAM 76
           +PL + NREMRILIAM
Sbjct: 133 NPLSQINREMRILIAM 148

The following BLAST results are available for this feature:

BLAST of Lsi11G006510 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi11G006510 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGJ6_CUCSA	1.6e-19	70.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G034680 PE=4 SV=1	[more]
A0A0A0LM47_CUCSA	3.5e-19	66.27	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G034690 PE=4 SV=1	[more]
A0A0D2ST66_GOSRA	1.9e-17	56.10	Uncharacterized protein OS=Gossypium raimondii GN=B456_007G298200 PE=4 SV=1	[more]
B9N451_POPTR	5.6e-17	53.01	Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa ...	[more]
B9RED7_RICCO	6.1e-16	53.09	Enzyme inhibitor, putative OS=Ricinus communis GN=RCOM_1620790 PE=4 SV=1	[more]

BLAST of Lsi11G006510 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G09360.1	1.4e-06	40.51	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Lsi11G006510 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659071554\|ref\|XP_008460590.1\|	5.0e-27	75.00	PREDICTED: uncharacterized protein LOC103499372 [Cucumis melo]	[more]
gi\|659071542\|ref\|XP_008460509.1\|	4.2e-26	77.38	PREDICTED: uncharacterized protein LOC103499305 [Cucumis melo]	[more]
gi\|659071552\|ref\|XP_008460578.1\|	2.1e-25	75.00	PREDICTED: uncharacterized protein LOC103499366 [Cucumis melo]	[more]
gi\|659071548\|ref\|XP_008460537.1\|	9.7e-23	69.88	PREDICTED: uncharacterized protein LOC103499331 [Cucumis melo]	[more]
gi\|659071550\|ref\|XP_008460566.1\|	8.2e-22	72.37	PREDICTED: uncharacterized protein LOC103499358 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0008150	biological_process
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G006510.1	Lsi11G006510.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 2..77 score: 3.
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 3..77 score: 5.
None	No IPR available	PANTHER	PTHR36710	FAMILY NOT NAMED	coord: 1..84 score: 7.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi11G006510	Csa2G034680	Cucumber (Chinese Long) v2	culsiB098
Lsi11G006510	CSPI02G04860	Wild cucumber (PI 183967)	cpilsiB096
Lsi11G006510	Cucsa.090480	Cucumber (Gy14) v1	cgylsiB100
Lsi11G006510	CmaCh05G010650	Cucurbita maxima (Rimu)	cmalsiB715
Lsi11G006510	CmoCh05G010750	Cucurbita moschata (Rifu)	cmolsiB706
Lsi11G006510	Cla019985	Watermelon (97103) v1	lsiwmB107
Lsi11G006510	MELO3C004166	Melon (DHL92) v3.5.1	lsimeB115
Lsi11G006510	ClCG02G014750	Watermelon (Charleston Gray)	lsiwcgB098
Lsi11G006510	MELO3C004156.2	Melon (DHL92) v3.6.1	lsimedB123
Lsi11G006510	Cla97C02G040540	Watermelon (97103) v2	lsiwmbB087
Lsi11G006510	Bhi06G001353	Wax gourd	lsiwgoB101
Lsi11G006510	Cp4.1LG11g08690	Cucurbita pepo (Zucchini)	cpelsiB075
Lsi11G006510	CsGy2G005000	Cucumber (Gy14) v2	cgyblsiB089
Lsi11G006510	Carg11993	Silver-seed gourd	carlsiB510
Lsi11G006510	Carg25590	Silver-seed gourd	carlsiB850
Lsi11G006510	CsaV3_2G007240	Cucumber (Chinese Long) v3	cuclsiB108

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G006510	Cucurbita moschata (Rifu)	cmolsiB034
Lsi11G006510	Cucurbita moschata (Rifu)	cmolsiB148
Lsi11G006510	Cucurbita moschata (Rifu)	cmolsiB331
Lsi11G006510	Cucurbita pepo (Zucchini)	cpelsiB659
Lsi11G006510	Melon (DHL92) v3.5.1	lsimeB105
Lsi11G006510	Watermelon (Charleston Gray)	lsiwcgB105
Lsi11G006510	Watermelon (Charleston Gray)	lsiwcgB111
Lsi11G006510	Watermelon (97103) v1	lsiwmB094
Lsi11G006510	Melon (DHL92) v3.6.1	lsimedB112
Lsi11G006510	Watermelon (97103) v2	lsiwmbB099
Lsi11G006510	Bottle gourd (USVL1VR-Ls)	lsilsiB002
Lsi11G006510	Cucurbita maxima (Rimu)	cmalsiB159