Lsi11G001310 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi11G001310
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionUracil-DNA glycosylase
Locationchr11 : 1461674 .. 1466863 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGACGAAGGCGCAGATCCCGTACGATCAAATTTCAAACCAGCGTGGAGAGAGGGAAGCTTTTAGCGGGATTCTTCATTGCTTCCCGCCATTGAAGCACAGCCCACTCCACTGCCCCAACCAGAACCGAAAATGGCTTCTTCCTCGGCTTCACTCTCATCCAAAACCAGAACCCTAATCGACATCTTCCAGCCAGCGCTTTCCAAACGCTTAAAAACCTCTCAGACATTGAAAACCCTCGCAACCACGGACGAGAAATGCGATTCAGAGCTCACATTGGCTTCCTCCTCCATGGACATGTCTGCCGCTCAGAAATCCCGCATGGAAACCAACAAATGGATGGCCAAATCGAAGCGCAATCTCAAAATGTGCTCAGATAGGGTTTCCAAATGGGAGAACGGATGTGTGAAGTTGGAGGAGCTTTTGGTGGACGAGACATGGTTCGAAGCCCTTCCCGGAGAGTTCGAGAAGCCCTATGCTCTTAATCTTTGCAAATTCGTAGAGACAGAGATTTGCAGCAGTGGTGTCCCTATTTATCCTCCTCCCTCTTTGATCTTTAATGCTCTGAATTCTACCCCTTTCGATAGGGTCAAAGTTGTTATTCTCGGTCAAGATCCTTATCATGGGCCTGGTCAAGCTATGGGTCTTTCATTTTCTGTTCCTGAGGGAGTTAAAATCCCATCTAGTCTTCTCAACATATTCAAGGAACTGAGGGAAGATCTTGGTTGTTCCATCCCGTCCCATGGAAATCTCGAGAAATGGGCTGTTCAGGTGCTGTTGTCTCTCATCTCTTTAGTCTGTGGACTCTGAAATGCATGTTTAAATGTAGCTACTTCTAATATATTGGTCTTGTGAATATTTTGGTGCTACAAAAATGCTACCTACCATTGGTTAAAGTACATGGGATGAATGTCTTGCTTATTTATCATTAGATGAAAGATATGTGAATGAATATAGGTTTGGGACAGATGGACTATATAATGGATATGGTTTATCACAAGGTTGTGATTTTGGTGATTACTAAGGGAATGAGGAAATGTTTTCTGATATTGGAGGCCTTTTCTTAAATTAAAAACATTACTTCTGAAGACAACTAATAAGCTAAGCTAGTTTCAATTTATTTATTTGTTATTATTATGTTTTTAAAAGAACTTGTTAGCAAGTTGAAAATTTGGGTTGTTACTAGGTGACTTCTCTAAGTTGAGAGGTCGTGTCATACAATTAGTACATATTGGCAAAGCATTCTAAATGAGGTTTGTTATGACACCACTCTTATGAATTTGGGCTTGTATGGCTTCGTAGGATAAGTGATCCATTCGGGAGATTTTTCGGTATGGAAAAATGCATTGCATTATTAGTTATTTTCTGAAAAAAAATTCTTTTGTTAGAATGTATTCGAAGTTCTATCTAGTGTATTTTTCACTATTCTGTTCTCTAAAATGATTTTTATGTACATTGTAGGGAGTCTTGTTGCTTAATGCTGTTCTCACAGGTAATAATCTTCTTTCCACCTAAAGTTTCATACCTTCTTTTTTCAGTCTACTTCTTTTATTTAAATCAGTAAAGTAAAGGTGAAAGTATTATGCAAAATGCTTTGTTTTGCTCTTTTTGATTGTTTTTAAATTTCATCATGAGTAACTCTTGACCATCTAAAAAACTTTGCAATAGCTAAATAACATTTTAAGTTTCCAGCTCCAAAGAATAATAGCAAACTTCAATGCCATCAGCAACATCTATGACATTGAACCAAAAACTCATCAAACATCACCAAACCGTCACAAAGAGAAACAACGCTGATAGATAGCCATTTCGTCAAAACCAAAGGCTAACTCTTGAAACCGAGATTTGCAACAACTAAAACAAATTTACTATCTTACTAAACTTGCTCTTAAACTGCACACCTTTTCCTAGGATAAGAAACAGCAAAAAGCTGCGAAATGGGTGATCTTCAACAAAAAACTTTATAAAGTTATAACATAACATTATTGCAACGGAAGCCCTTGGGGAACAAGCTAGATAATGCTCCAGTCAACAAAATAGCCATCATAGAAAACCAACAACATCAGACCCAAAGAAAGCCAAAGCGCCCTTTCCAAGATTGCCATAACCAAACTAAAAAGAACTGAAAACAAACGGAAACCCAGACTTTGGATAGACACTCAGTGGAATCAACTCCTCGAGAAGAAACACTATAAAGGGTGAAGTTTGTTGTTCTATCCCATGTGTATCAAACATTAATAGGAGTTCTTCTATTTTTAGGTAAAGATACATGCTTCCTGCATTTAGTTATTGATTAATATTTTGGTTTTACCTGTTTAGTTCGAAAGCATCAAGCCAACTCCCATGCTAAGAAAGGATGGGAACAATTCACTGATGCTGTCATCAAGACAATATCACAAAAGAAGGAAGGGATTATCTTTCTACTTTGGGGGAACTCTGCTCAGGCAAAATTGAGGTACTTCTATCGTATGATTTTCGTTCTAACAGGGAGGTTCATGACTCCGGAAAAAACCAATATTACGGAATCGATATTAAAATATAAATAGCAACCTTTTGATTTTAATTTTTGGAAAAAAATAAAAGCAGTAACCTTTGATTAAAATTTTTGCATCTAGTATGCACTTTTATTAACTTCCATATTTATCAACCCACTATCATCATACTCCATCCAAACATTCCAAACCACTCAAACCAATGTTTTAAAAGATCAAAATATAAGAAGAACCACGCTCACTCAACTAATTTCGTAGTCAGAGAACCTAAAAAACTTTGCAAAGATTAGATGCTCTCTAAATACCATTAGCAAACTCCAGCCAAATTAGACAAACAAAATACAAAGTTGGACACGAAGAATACGAGGGTAGAGATAAAGTAGACGACAGTGATGAAGAAGCCGAAGATAGTGATATTAAAGAAATTGAATAAGACGACACTTATTATAGAGAAGAGCTCTAACAAATGCGTTGATTTCTTTCCAACCAAAGCTCAGAAAGGATGGCCTTGACAACATTCACCCAAGGTAAATAAGCTTGAGAATGCCACTTAGTCCCACATAAAAGCTAGAGCACACTTCCCCTTGAAGACTTTGAGAAAACCCAACTGAGGTTGAAGATATCAAATAATAAAAACCAGCATTGCTGAGAGTATAAACAGCCAAAGAAGATGTGGCCGAGATATTCCCTTGCTTTAAAACAAAGAAAACAAACTGAAGGTAGAAGACAATGCGAAGGATTCTTCCTTCGTAAGATATCAGGCTTTCAGCCGAGTTGAAACTTCCATTAAGCATAATTCAAACAAGAATAATTATTTTCTTTGGACTCTTTGTCTTCTATAATGCTCTAATAAGTCATTATCCATCAAAGAAGAAGAAGCCAAGTGATGTGATAAAGACTTAACTGTGAAGGAACCTGATGATTCAAGAGACCAAATTCAAGAATTGCTGCAAATTCACTCATCATTTCTTCCAAAGTGTTATGCATTGTCAGCAATATTCTCCTAGACTCTGAATTCTCAAATATTGAATTGCTTTAGTACCAATCTGATAGGATCGATGTAGTAGAAATTAATAAAAACTATTATCAAATTAGAAAGGAAAAATGCATGATCGAAGTACTTTAGTACCACTCAACTTTTACCACTCAACTTTGACGAACTTATATTAAAAATTATAATGAATATGCCATGGGGATATTCCTTACATTTCTCAATTACATTTCATATTAATAGCTCACATATTAAACCTCATCCAAATGTAAAGCTGACACCTACAGTTGAGGGGATGTGTTGTAAATATTTTACCTCAGTAGTCATTTGTTACCTATCTTGTTATGCAGACTCTAAGCTTATAAGTAAACTTTTCCTGTGAAGTACAGGAAACTCTTTGGGAAAACATGGTTTTGCTGAGTTTTAAAATCAACGTAGTGCAATTTTCATTTACTTTAGCTAGCTTAGGTTCTATTGTCTTGATGAAACTAACAAGAGCATCATAGAAAGGGTTTTATTCCTCTTCTTTGCTGGGCAATAGCATCCTACTTCACTTAGAATCTGATGTATCAACAGGTTAATTGATGAGAAAAAGCATCATATTCTCAAAGCAGCGCATCCTTCTGGTTTGTCTGCTAACAGAGGCTTCTTTGGTTGCAGGTTAGTTTCATCGTTTAATTCCATCAGAAATTTTTTAAACATTCCTTTTGTTCAAATATTAACTGGGATTTGTTCTAATCTTTTTACTTTATCCCCTTCAGTCTAGTTCTCACAATATCAACATTTTATTGCCAGTATGCGACTTGGTGTTATTTTGTGTATTATTGGTGTTCTGCCTTCTCTAAATCAAAGTTCTTAAGTTGGAGCACTTTTTTGCTCCAATTTGTATCTGGTCGGAGCGTGAATTTGGTATGACTTTTCTGAATGGTAAAAAACATTTTTAGCCAATCCCAAACAAACATTTTTGAAATTTTTTATATAGTTTTGATCTTTGAAAAGAATTCTCAAAATACTTAAAAAGCACCTTTTGGAACATAGTTGTTTATAAATAAAATGTCACTTAAAAGTATTTGTTCTCAAGAATTATACCAAACTCCCACTTATCTTTCCTTAAGTCTTGTGGTAGACAATCTCGAACATAGAAGTTTATAATTGCAAGAGAGAAAATGTTTATGTATTTCACTAATATGAAGCTATGTAGATGGGTGCCTTTTCATCTCTCTTGGAATGGTTAGTTTGTTTACTGCCAACTTTGGATTTTCGACAGGCATTTTTCTCGAACAAACGTGCTTCTCAAGGAACTAGGTACTGCCACCATAGACTGGCAACTTTGATCCAAGCACCTGCTTGAACCATTGAAGTTCATTTTGTGGATACAGTTCCAAATCCCTGGTCATGCTAGGATCTGGTCGACGACTTTCATTGAGTTTTCACTTGTCGATTCACTGTTGTTGAAATGTCGAGATGCCCATCCTTTACCATTTTTGTATTATAGTGTTTATTCATGCTGTTCGATATTTATCCCAAAGCAGCAACAGCACCCTTCTTTTTGATGAAACAGGGGTAAAGTTAGAAGAAAGTTGATCTATAATCTGGTAGCTAGTGGTAAATTTGAGTTTAGAAACAAGTTTTATCAGACGTAAATTTATTCCATCTTCTTCCTCAGAATAAATCTTGATTTAGGAGCTTTAAAATTTGTGATCTAGG

mRNA sequence

AAAGACGAAGGCGCAGATCCCGTACGATCAAATTTCAAACCAGCGTGGAGAGAGGGAAGCTTTTAGCGGGATTCTTCATTGCTTCCCGCCATTGAAGCACAGCCCACTCCACTGCCCCAACCAGAACCGAAAATGGCTTCTTCCTCGGCTTCACTCTCATCCAAAACCAGAACCCTAATCGACATCTTCCAGCCAGCGCTTTCCAAACGCTTAAAAACCTCTCAGACATTGAAAACCCTCGCAACCACGGACGAGAAATGCGATTCAGAGCTCACATTGGCTTCCTCCTCCATGGACATGTCTGCCGCTCAGAAATCCCGCATGGAAACCAACAAATGGATGGCCAAATCGAAGCGCAATCTCAAAATGTGCTCAGATAGGGTTTCCAAATGGGAGAACGGATGTGTGAAGTTGGAGGAGCTTTTGGTGGACGAGACATGGTTCGAAGCCCTTCCCGGAGAGTTCGAGAAGCCCTATGCTCTTAATCTTTGCAAATTCGTAGAGACAGAGATTTGCAGCAGTGGTGTCCCTATTTATCCTCCTCCCTCTTTGATCTTTAATGCTCTGAATTCTACCCCTTTCGATAGGGTCAAAGTTGTTATTCTCGGTCAAGATCCTTATCATGGGCCTGGTCAAGCTATGGGTCTTTCATTTTCTGTTCCTGAGGGAGTTAAAATCCCATCTAGTCTTCTCAACATATTCAAGGAACTGAGGGAAGATCTTGGTTGTTCCATCCCGTCCCATGGAAATCTCGAGAAATGGGCTGTTCAGGGAGTCTTGTTGCTTAATGCTGTTCTCACAGTTCGAAAGCATCAAGCCAACTCCCATGCTAAGAAAGGATGGGAACAATTCACTGATGCTGTCATCAAGACAATATCACAAAAGAAGGAAGGGATTATCTTTCTACTTTGGGGGAACTCTGCTCAGGCAAAATTGAGGTTAATTGATGAGAAAAAGCATCATATTCTCAAAGCAGCGCATCCTTCTGGTTTGTCTGCTAACAGAGGCTTCTTTGGTTGCAGGCATTTTTCTCGAACAAACGTGCTTCTCAAGGAACTAGGTACTGCCACCATAGACTGGCAACTTTGATCCAAGCACCTGCTTGAACCATTGAAGTTCATTTTGTGGATACAGTTCCAAATCCCTGGTCATGCTAGGATCTGGTCGACGACTTTCATTGAGTTTTCACTTGTCGATTCACTGTTGTTGAAATGTCGAGATGCCCATCCTTTACCATTTTTGTATTATAGTGTTTATTCATGCTGTTCGATATTTATCCCAAAGCAGCAACAGCACCCTTCTTTTTGATGAAACAGGGGTAAAGTTAGAAGAAAGTTGATCTATAATCTGGTAGCTAGTGGTAAATTTGAGTTTAGAAACAAGTTTTATCAGACGTAAATTTATTCCATCTTCTTCCTCAGAATAAATCTTGATTTAGGAGCTTTAAAATTTGTGATCTAGG

Coding sequence (CDS)

ATGGCTTCTTCCTCGGCTTCACTCTCATCCAAAACCAGAACCCTAATCGACATCTTCCAGCCAGCGCTTTCCAAACGCTTAAAAACCTCTCAGACATTGAAAACCCTCGCAACCACGGACGAGAAATGCGATTCAGAGCTCACATTGGCTTCCTCCTCCATGGACATGTCTGCCGCTCAGAAATCCCGCATGGAAACCAACAAATGGATGGCCAAATCGAAGCGCAATCTCAAAATGTGCTCAGATAGGGTTTCCAAATGGGAGAACGGATGTGTGAAGTTGGAGGAGCTTTTGGTGGACGAGACATGGTTCGAAGCCCTTCCCGGAGAGTTCGAGAAGCCCTATGCTCTTAATCTTTGCAAATTCGTAGAGACAGAGATTTGCAGCAGTGGTGTCCCTATTTATCCTCCTCCCTCTTTGATCTTTAATGCTCTGAATTCTACCCCTTTCGATAGGGTCAAAGTTGTTATTCTCGGTCAAGATCCTTATCATGGGCCTGGTCAAGCTATGGGTCTTTCATTTTCTGTTCCTGAGGGAGTTAAAATCCCATCTAGTCTTCTCAACATATTCAAGGAACTGAGGGAAGATCTTGGTTGTTCCATCCCGTCCCATGGAAATCTCGAGAAATGGGCTGTTCAGGGAGTCTTGTTGCTTAATGCTGTTCTCACAGTTCGAAAGCATCAAGCCAACTCCCATGCTAAGAAAGGATGGGAACAATTCACTGATGCTGTCATCAAGACAATATCACAAAAGAAGGAAGGGATTATCTTTCTACTTTGGGGGAACTCTGCTCAGGCAAAATTGAGGTTAATTGATGAGAAAAAGCATCATATTCTCAAAGCAGCGCATCCTTCTGGTTTGTCTGCTAACAGAGGCTTCTTTGGTTGCAGGCATTTTTCTCGAACAAACGTGCTTCTCAAGGAACTAGGTACTGCCACCATAGACTGGCAACTTTGA

Protein sequence

MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQKSRMETNKWMAKSKRNLKMCSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL
BLAST of Lsi11G001310 vs. Swiss-Prot
Match: UNG_ARATH (Uracil-DNA glycosylase, mitochondrial OS=Arabidopsis thaliana GN=UNG PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 6.1e-110
Identity = 210/330 (63.64%), Postives = 246/330 (74.55%), Query Frame = 1

Query: 9   SSKTRTLIDIFQPALSKRLKTSQT---------------LKTLATTDEKCDSELTLASSS 68
           SS  +TL+D FQPA  KRLK S +               L ++A +  +     ++A  S
Sbjct: 3   SSTPKTLMDFFQPA--KRLKASPSSSSFPAVSVAGGSRDLGSVANSPPRVTVTTSVADDS 62

Query: 69  MDMSAAQKSRMETNKWMAKSKRNLKMCSDRVSKW--ENGC-VKLEELLVDETWFEALPGE 128
             ++  Q +R E NK++AKSKRNL +CS+RV+K   E  C V L ELLV+E+W +ALPGE
Sbjct: 63  SGLTPEQIARAEFNKFVAKSKRNLAVCSERVTKAKSEGNCYVPLSELLVEESWLKALPGE 122

Query: 129 FEKPYALNLCKFVETEIC--SSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQ 188
           F KPYA +L  F+E EI   S    IYPP  LIFNALN+TPFDRVK VI+GQDPYHGPGQ
Sbjct: 123 FHKPYAKSLSDFLEREIITDSKSPLIYPPQHLIFNALNTTPFDRVKTVIIGQDPYHGPGQ 182

Query: 189 AMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQ 248
           AMGLSFSVPEG K+PSSLLNIFKEL +D+GCSIP HGNL+KWAVQGVLLLNAVLTVR  Q
Sbjct: 183 AMGLSFSVPEGEKLPSSLLNIFKELHKDVGCSIPRHGNLQKWAVQGVLLLNAVLTVRSKQ 242

Query: 249 ANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLS 308
            NSHAKKGWEQFTDAVI++ISQ+KEG++FLLWG  AQ K +LID  KHHIL AAHPSGLS
Sbjct: 243 PNSHAKKGWEQFTDAVIQSISQQKEGVVFLLWGRYAQEKSKLIDATKHHILTAAHPSGLS 302

Query: 309 ANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           ANRGFF CRHFSR N LL+E+G   IDWQL
Sbjct: 303 ANRGFFDCRHFSRANQLLEEMGIPPIDWQL 330

BLAST of Lsi11G001310 vs. Swiss-Prot
Match: UNG_AZOVD (Uracil-DNA glycosylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=ung PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.3e-72
Identity = 130/224 (58.04%), Postives = 163/224 (72.77%), Query Frame = 1

Query: 95  EELLVDETWFEALPGEFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + ++ +W EAL  EFEKPY   L  F+  E  ++G  IYPP SLIFNAL+STP D+VK
Sbjct: 6   DRVRLEASWKEALHDEFEKPYMQELSDFLRREK-AAGKEIYPPGSLIFNALDSTPLDQVK 65

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQG 214
           VVI+GQDPYHGPGQA GL FSV  GV +P SL NIFKEL+ DL   IP HG+L++WA QG
Sbjct: 66  VVIIGQDPYHGPGQAHGLCFSVQPGVPVPPSLQNIFKELKRDLNIDIPKHGHLQRWAEQG 125

Query: 215 VLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEK 274
           VLLLN  LTV +  A SHA  GW++FTD VI+ +SQ++E ++F+LWG+ AQ+K RLID  
Sbjct: 126 VLLLNTSLTVERGNAGSHAGMGWQRFTDRVIEVVSQRREHVVFMLWGSHAQSKRRLIDSS 185

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           KH +L +AHPS LSA+RGF G  HFSR N  L++ G   IDW L
Sbjct: 186 KHLVLCSAHPSPLSAHRGFIGNGHFSRANQFLEQHGLTPIDWHL 228

BLAST of Lsi11G001310 vs. Swiss-Prot
Match: UNG_PSEPK (Uracil-DNA glycosylase OS=Pseudomonas putida (strain KT2440) GN=ung PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.6e-72
Identity = 129/224 (57.59%), Postives = 160/224 (71.43%), Query Frame = 1

Query: 95  EELLVDETWFEALPGEFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + ++ +W  AL GEF++PY   L +F+  E  ++G  IYPP  LIFNALNSTP D+VK
Sbjct: 5   DRIKLEPSWKAALRGEFDQPYMHQLREFLRGEY-AAGKEIYPPGPLIFNALNSTPLDQVK 64

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQG 214
           VVILGQDPYHGPGQA GL FSV  GV  P SL+NI+KEL+ DL   IPSHG L+ WA QG
Sbjct: 65  VVILGQDPYHGPGQAHGLCFSVQPGVATPPSLVNIYKELQRDLNIPIPSHGYLQSWAEQG 124

Query: 215 VLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEK 274
           VLLLN  +TV +  A SHAKKGWE FTD +I+ +S++   ++FLLWG  AQ+K +LID  
Sbjct: 125 VLLLNTTMTVERANAASHAKKGWELFTDRIIQVVSEQCPNVVFLLWGAHAQSKQKLIDGT 184

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           KH +LK+ HPS LSA RGF GC HFSR N  L++ G   IDW L
Sbjct: 185 KHLVLKSVHPSPLSAYRGFIGCGHFSRANSFLEQRGLGPIDWAL 227

BLAST of Lsi11G001310 vs. Swiss-Prot
Match: UNG_PSEP1 (Uracil-DNA glycosylase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ung PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-71
Identity = 129/224 (57.59%), Postives = 160/224 (71.43%), Query Frame = 1

Query: 95  EELLVDETWFEALPGEFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + ++ +W  AL GEF++PY   L +F+  E  ++G  IYPP  LIFNALNSTP  +VK
Sbjct: 5   DRIKLEPSWKAALRGEFDQPYMHQLREFLRGEY-AAGKEIYPPGPLIFNALNSTPLGQVK 64

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQG 214
           VVILGQDPYHGPGQA GL FSV  GV  P SL+NI+KEL+ DL   IPSHG L+ WA QG
Sbjct: 65  VVILGQDPYHGPGQAHGLCFSVQPGVATPPSLVNIYKELQRDLNIPIPSHGYLQSWAEQG 124

Query: 215 VLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEK 274
           VLLLN  +TV +  A SHAKKGWE FTD +I+ +S++   ++FLLWG  AQ+K +LID  
Sbjct: 125 VLLLNTTMTVERANAASHAKKGWELFTDRIIQVVSEQCPNVVFLLWGAHAQSKQKLIDGT 184

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           KH +LK+ HPS LSA RGF GC HFSRTN  L++ G   IDW L
Sbjct: 185 KHLVLKSVHPSPLSAYRGFIGCGHFSRTNSFLEQRGLGPIDWAL 227

BLAST of Lsi11G001310 vs. Swiss-Prot
Match: UNG_PSEPG (Uracil-DNA glycosylase OS=Pseudomonas putida (strain GB-1) GN=ung PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.5e-71
Identity = 130/224 (58.04%), Postives = 161/224 (71.88%), Query Frame = 1

Query: 95  EELLVDETWFEALPGEFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + ++ +W  AL GEF++PY   L +F+  E  ++G  IYPP  LIFNALNSTP D+VK
Sbjct: 5   DRIKLEPSWKAALRGEFDQPYMHQLREFLRGEY-AAGKEIYPPGPLIFNALNSTPLDQVK 64

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQG 214
           VVILGQDPYHGPGQA GL FSV  GV  P SL+NI+KEL+ DL   I SHG L+ WA QG
Sbjct: 65  VVILGQDPYHGPGQAHGLCFSVQPGVATPPSLVNIYKELQRDLNIPIASHGYLQSWAEQG 124

Query: 215 VLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEK 274
           VLLLN  +TV +  A SHAKKGWE FTD VI+ +S++   ++FLLWG  AQ+K +LID  
Sbjct: 125 VLLLNTTMTVERANAASHAKKGWEFFTDRVIQVVSEQCPNVVFLLWGAHAQSKQKLIDGT 184

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           KH +LK+ HPS LSA RGF GC HFSRTN  L++ G A I+W L
Sbjct: 185 KHLVLKSVHPSPLSAYRGFLGCGHFSRTNSFLEQRGMAPINWAL 227

BLAST of Lsi11G001310 vs. TrEMBL
Match: A0A0A0KMG3_CUCSA (Uracil-DNA glycosylase OS=Cucumis sativus GN=Csa_5G289610 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 7.4e-171
Identity = 298/318 (93.71%), Postives = 313/318 (98.43%), Query Frame = 1

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT D+KCDS+LTLASSS D+SA+Q
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSADISASQ 60

Query: 61  KSRMETNKWMAKSKRNLKMCSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLC 120
            SRMETNKW+A+SKRNLK CSDRVSKWENGCVKLEELLV+ETWFEALPGEF+KPYALNLC
Sbjct: 61  ISRMETNKWIARSKRNLKTCSDRVSKWENGCVKLEELLVEETWFEALPGEFQKPYALNLC 120

Query: 121 KFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQF 240
           KIPSSLLNIFKELR+DLGCSIPSHGNL KWAVQGVLLLNAVL+VRKHQANSHAK+GWEQF
Sbjct: 181 KIPSSLLNIFKELRDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNVLLKELGTATIDWQL 319
           RTN+LLKE+GTA+IDWQL
Sbjct: 301 RTNILLKEMGTASIDWQL 318

BLAST of Lsi11G001310 vs. TrEMBL
Match: W9RJ98_9ROSA (Uracil-DNA glycosylase OS=Morus notabilis GN=L484_009862 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.0e-124
Identity = 233/329 (70.82%), Postives = 268/329 (81.46%), Query Frame = 1

Query: 8   LSSKTRTLIDIFQPAL---SKRLKTSQTLKTLATTDEKCDSELTLASSSMD--------- 67
           ++SK +TL D F P     +KRLK     +TL++T+ KCD+   + + S           
Sbjct: 1   MASKAKTLTDFFPPLQQPSAKRLK-----QTLSSTNNKCDANGIIPNRSSSSSGIGDGGA 60

Query: 68  --MSAAQKSRMETNKWMAKSKRNLKMCSDRVS--KWENGC--VKLEELLVDETWFEALPG 127
             +SA QKSRME  K +AKS+RNLK+CS RVS  + E GC  VKLEELLV+E+W EALPG
Sbjct: 61  DGLSADQKSRMEFQKVLAKSRRNLKICSQRVSNSQSEGGCGYVKLEELLVEESWLEALPG 120

Query: 128 EFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQA 187
           EF+KPYA NL KF+E+E  + GV +YPP  LIFNALNSTPFDRVK VILGQDPYHG GQA
Sbjct: 121 EFQKPYAKNLSKFLESETSAVGVTVYPPSHLIFNALNSTPFDRVKAVILGQDPYHGLGQA 180

Query: 188 MGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQA 247
           MGLSFSVPEGVK+PSSL+NIFKEL++D+GCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQA
Sbjct: 181 MGLSFSVPEGVKVPSSLVNIFKELKQDVGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQA 240

Query: 248 NSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSA 307
           NSHAKKGWEQFTDAVIKTISQ+KEG++FLLWGNSAQ K RLIDE KHHILKAAHPSGLSA
Sbjct: 241 NSHAKKGWEQFTDAVIKTISQRKEGVVFLLWGNSAQEKRRLIDESKHHILKAAHPSGLSA 300

Query: 308 NRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           NRGFFGCRHFSRTN LL+++G  +IDWQL
Sbjct: 301 NRGFFGCRHFSRTNELLEKMGIPSIDWQL 324

BLAST of Lsi11G001310 vs. TrEMBL
Match: M5XNR7_PRUPE (Uracil-DNA glycosylase OS=Prunus persica GN=PRUPE_ppa022483mg PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 4.0e-124
Identity = 227/313 (72.52%), Postives = 254/313 (81.15%), Query Frame = 1

Query: 10  SKTRTLIDIFQPALS--KRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQKSRMETN 69
           +K +TL+D+FQP  S  KRLKT     T + +           SSS D++A QKSRME  
Sbjct: 5   NKNKTLLDLFQPTASSAKRLKTDSIRATHSDSVSPVPPPSHDDSSSSDLTAQQKSRMEFQ 64

Query: 70  KWMAKSKRNLKMCSDRVSKWENGC--VKLEELLVDETWFEALPGEFEKPYALNLCKFVET 129
           K +AK++RNL +CS+R+S   +    VKLEELLV+ETW EA P E +KPYA  L KFVE 
Sbjct: 65  KLLAKARRNLSICSNRLSNSNSKGEGVKLEELLVEETWLEAFPSELQKPYAKTLSKFVEN 124

Query: 130 EICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSS 189
           EIC   +PIYPP  LIFNALNSTPFDRVK VILGQDPYHGPGQAMGLSFSVPEGVK+PSS
Sbjct: 125 EICGGALPIYPPTHLIFNALNSTPFDRVKAVILGQDPYHGPGQAMGLSFSVPEGVKVPSS 184

Query: 190 LLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVI 249
           L+NIFKEL +DLGCSIPSHGNLEKWAVQGVLLLNAVLTVR HQANSHAKKGWEQFTDAVI
Sbjct: 185 LVNIFKELHQDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRNHQANSHAKKGWEQFTDAVI 244

Query: 250 KTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFSRTNVL 309
           KTISQK+EG++FLLWGNSAQ K +LIDE KHHILKAAHPSGLSANRGFFGCRHFSRTN L
Sbjct: 245 KTISQKREGVVFLLWGNSAQQKSKLIDESKHHILKAAHPSGLSANRGFFGCRHFSRTNQL 304

Query: 310 LKELGTATIDWQL 319
           L+E+G   IDWQL
Sbjct: 305 LEEMGIPPIDWQL 317

BLAST of Lsi11G001310 vs. TrEMBL
Match: A0A061E9U8_THECC (Uracil-DNA glycosylase OS=Theobroma cacao GN=TCM_011079 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 2.2e-122
Identity = 227/317 (71.61%), Postives = 259/317 (81.70%), Query Frame = 1

Query: 7   SLSSKTRTLIDIFQ--PALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQKSRM 66
           ++++ ++T+ D FQ  P  +KR K S       + D +    LT         A QKSRM
Sbjct: 16  AMAASSKTITDFFQANPGPAKRQKLSTP-----SDDHQPFPSLT---------AEQKSRM 75

Query: 67  ETNKWMAKSKRNLKMCSDRVSKWE---NGCVKLEELLVDETWFEALPGEFEKPYALNLCK 126
           E NK +AKSKRNLK+CS +VS+ +   +G VKLEELLV++TW EALPGE +KPYA NLCK
Sbjct: 76  EFNKCVAKSKRNLKICSQKVSQSKVEGSGFVKLEELLVEDTWLEALPGELQKPYANNLCK 135

Query: 127 FVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGVK 186
           FVE+EI S  VPIYPP  LIFNALNSTPF RVK VI+GQDPYHGPGQAMGLSFSVPEGVK
Sbjct: 136 FVESEISSGSVPIYPPQHLIFNALNSTPFHRVKAVIIGQDPYHGPGQAMGLSFSVPEGVK 195

Query: 187 IPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQFT 246
           +PSSL+NIFKEL++DLGCSIPS GNLEKWAVQGVLLLN VLTVRKHQANSHAKKGWEQFT
Sbjct: 196 VPSSLVNIFKELKQDLGCSIPSDGNLEKWAVQGVLLLNTVLTVRKHQANSHAKKGWEQFT 255

Query: 247 DAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFSR 306
           DA+I+TISQKKEG+IFLLWGNSAQ K RLID+KKHHILKAAHPSGLSANRGFFGCRHFSR
Sbjct: 256 DAIIRTISQKKEGVIFLLWGNSAQEKSRLIDQKKHHILKAAHPSGLSANRGFFGCRHFSR 315

Query: 307 TNVLLKELGTATIDWQL 319
           TN LL+++G   IDWQL
Sbjct: 316 TNQLLEQMGIPPIDWQL 318

BLAST of Lsi11G001310 vs. TrEMBL
Match: A0A0B0MRI6_GOSAR (Uracil-DNA glycosylase OS=Gossypium arboreum GN=F383_28725 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 7.0e-121
Identity = 224/308 (72.73%), Postives = 251/308 (81.49%), Query Frame = 1

Query: 13  RTLIDIFQP--ALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQKSRMETNKWM 72
           +T+ D F P  A +KR K S       ++D +  S LT         A QKSR+E NK +
Sbjct: 28  KTITDFFNPNPAPAKRRKLS------TSSDHQPFSSLT---------ADQKSRIELNKCL 87

Query: 73  AKSKRNLKMCSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLCKFVETEICSS 132
           A SKRNLK+CS +V    +G VKLEELLV++TW + LPGEF+KPYALNLCKFVE E+ S 
Sbjct: 88  AISKRNLKLCSQKVE--GSGYVKLEELLVEDTWLQVLPGEFQKPYALNLCKFVEAELSSG 147

Query: 133 GVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIF 192
            VPI+PP  LIFNALNSTPF RVKVVI+GQDPYHGPGQAMGLSFSVPEGVKIPSSL NIF
Sbjct: 148 AVPIFPPQHLIFNALNSTPFHRVKVVIIGQDPYHGPGQAMGLSFSVPEGVKIPSSLANIF 207

Query: 193 KELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQ 252
           KEL++DLGCSIPSHGNL KWAVQGVLLLN VLTVRK QANSHAKKGWEQFTDAVIKTISQ
Sbjct: 208 KELKQDLGCSIPSHGNLHKWAVQGVLLLNTVLTVRKQQANSHAKKGWEQFTDAVIKTISQ 267

Query: 253 KKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELG 312
           KKEG++FLLWGNSAQ K +LID+ KHHILKAAHPSGLSANRGFFGCRHFS TN LL+++G
Sbjct: 268 KKEGVVFLLWGNSAQEKSKLIDQTKHHILKAAHPSGLSANRGFFGCRHFSCTNQLLEQMG 318

Query: 313 TATIDWQL 319
           TA IDWQL
Sbjct: 328 TAPIDWQL 318

BLAST of Lsi11G001310 vs. TAIR10
Match: AT3G18630.1 (AT3G18630.1 uracil dna glycosylase)

HSP 1 Score: 398.7 bits (1023), Expect = 3.4e-111
Identity = 210/330 (63.64%), Postives = 246/330 (74.55%), Query Frame = 1

Query: 9   SSKTRTLIDIFQPALSKRLKTSQT---------------LKTLATTDEKCDSELTLASSS 68
           SS  +TL+D FQPA  KRLK S +               L ++A +  +     ++A  S
Sbjct: 3   SSTPKTLMDFFQPA--KRLKASPSSSSFPAVSVAGGSRDLGSVANSPPRVTVTTSVADDS 62

Query: 69  MDMSAAQKSRMETNKWMAKSKRNLKMCSDRVSKW--ENGC-VKLEELLVDETWFEALPGE 128
             ++  Q +R E NK++AKSKRNL +CS+RV+K   E  C V L ELLV+E+W +ALPGE
Sbjct: 63  SGLTPEQIARAEFNKFVAKSKRNLAVCSERVTKAKSEGNCYVPLSELLVEESWLKALPGE 122

Query: 129 FEKPYALNLCKFVETEIC--SSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQ 188
           F KPYA +L  F+E EI   S    IYPP  LIFNALN+TPFDRVK VI+GQDPYHGPGQ
Sbjct: 123 FHKPYAKSLSDFLEREIITDSKSPLIYPPQHLIFNALNTTPFDRVKTVIIGQDPYHGPGQ 182

Query: 189 AMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQ 248
           AMGLSFSVPEG K+PSSLLNIFKEL +D+GCSIP HGNL+KWAVQGVLLLNAVLTVR  Q
Sbjct: 183 AMGLSFSVPEGEKLPSSLLNIFKELHKDVGCSIPRHGNLQKWAVQGVLLLNAVLTVRSKQ 242

Query: 249 ANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLS 308
            NSHAKKGWEQFTDAVI++ISQ+KEG++FLLWG  AQ K +LID  KHHIL AAHPSGLS
Sbjct: 243 PNSHAKKGWEQFTDAVIQSISQQKEGVVFLLWGRYAQEKSKLIDATKHHILTAAHPSGLS 302

Query: 309 ANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           ANRGFF CRHFSR N LL+E+G   IDWQL
Sbjct: 303 ANRGFFDCRHFSRANQLLEEMGIPPIDWQL 330

BLAST of Lsi11G001310 vs. NCBI nr
Match: gi|659130542|ref|XP_008465227.1| (PREDICTED: uracil-DNA glycosylase isoform X2 [Cucumis melo])

HSP 1 Score: 609.4 bits (1570), Expect = 3.6e-171
Identity = 298/318 (93.71%), Postives = 314/318 (98.74%), Query Frame = 1

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT D+KCDS+LTLASSS DMSA+Q
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSTDMSASQ 60

Query: 61  KSRMETNKWMAKSKRNLKMCSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLC 120
            SRMETNKWMA+SKRNLK+CSDRVSKWENGC+KLEELLV+ETWFEALPGEFEKPYALNLC
Sbjct: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCMKLEELLVEETWFEALPGEFEKPYALNLC 120

Query: 121 KFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQF 240
           KIPSSLLNIFKEL++DLGCSIPSHGNL KWAVQGVLLLNAVL+VR+HQANSHAK+GWEQF
Sbjct: 181 KIPSSLLNIFKELKDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVREHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGI+FLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNVLLKELGTATIDWQL 319
           RTN+LLKELGTA+IDWQL
Sbjct: 301 RTNILLKELGTASIDWQL 318

BLAST of Lsi11G001310 vs. NCBI nr
Match: gi|449445338|ref|XP_004140430.1| (PREDICTED: uracil-DNA glycosylase isoform X1 [Cucumis sativus])

HSP 1 Score: 607.8 bits (1566), Expect = 1.1e-170
Identity = 298/318 (93.71%), Postives = 313/318 (98.43%), Query Frame = 1

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT D+KCDS+LTLASSS D+SA+Q
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSADISASQ 60

Query: 61  KSRMETNKWMAKSKRNLKMCSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLC 120
            SRMETNKW+A+SKRNLK CSDRVSKWENGCVKLEELLV+ETWFEALPGEF+KPYALNLC
Sbjct: 61  ISRMETNKWIARSKRNLKTCSDRVSKWENGCVKLEELLVEETWFEALPGEFQKPYALNLC 120

Query: 121 KFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQF 240
           KIPSSLLNIFKELR+DLGCSIPSHGNL KWAVQGVLLLNAVL+VRKHQANSHAK+GWEQF
Sbjct: 181 KIPSSLLNIFKELRDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNVLLKELGTATIDWQL 319
           RTN+LLKE+GTA+IDWQL
Sbjct: 301 RTNILLKEMGTASIDWQL 318

BLAST of Lsi11G001310 vs. NCBI nr
Match: gi|659130540|ref|XP_008465225.1| (PREDICTED: uracil-DNA glycosylase isoform X1 [Cucumis melo])

HSP 1 Score: 595.5 bits (1534), Expect = 5.4e-167
Identity = 298/343 (86.88%), Postives = 314/343 (91.55%), Query Frame = 1

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT D+KCDS+LTLASSS DMSA+Q
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSTDMSASQ 60

Query: 61  KSRMETNKWMAKSKRNLKMCSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLC 120
            SRMETNKWMA+SKRNLK+CSDRVSKWENGC+KLEELLV+ETWFEALPGEFEKPYALNLC
Sbjct: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCMKLEELLVEETWFEALPGEFEKPYALNLC 120

Query: 121 KFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLT----------------- 240
           KIPSSLLNIFKEL++DLGCSIPSHGNL KWAVQGVLLLNAVL+                 
Sbjct: 181 KIPSSLLNIFKELKDDLGCSIPSHGNLGKWAVQGVLLLNAVLSATSRILNQKLFKHHQTV 240

Query: 241 --------VRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKK 300
                   VR+HQANSHAK+GWEQFTDAVIKTISQKKEGI+FLLWGNSAQAKLRLIDEKK
Sbjct: 241 MKSNKADIVREHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK 300

Query: 301 HHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           HHILKAAHPSGLSANRGFFGCRHFSRTN+LLKELGTA+IDWQL
Sbjct: 301 HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 343

BLAST of Lsi11G001310 vs. NCBI nr
Match: gi|1009106111|ref|XP_015870160.1| (PREDICTED: uracil-DNA glycosylase, mitochondrial [Ziziphus jujuba])

HSP 1 Score: 461.5 bits (1186), Expect = 1.2e-126
Identity = 242/349 (69.34%), Postives = 276/349 (79.08%), Query Frame = 1

Query: 1   MASSSASLSSKTRTLIDIFQP-----ALSKRLKTS-----------------QTLKTLAT 60
           MAS ++S     +TL DIF+P     + +KRLK S                 Q + +L+ 
Sbjct: 1   MASRASSEIKTRKTLSDIFRPQHPAASAAKRLKPSSLGFSGSKQPPNPIHHCQGVASLSK 60

Query: 61  TDEKC-------DSELTLASSSMDMSAAQKSRMETNKWMAKSKRNLKMCSDRVSKWENGC 120
            D+         DSE + +SSS  ++  Q SRME ++ +AK+KRN K CS RVSK + G 
Sbjct: 61  CDDDGGVLPIPNDSESSRSSSSA-LTDQQISRMEFHRLLAKAKRNQKTCSGRVSKCKGGS 120

Query: 121 --VKLEELLVDETWFEALPGEFEKPYALNLCKFVETEICSSGVPIYPPPSLIFNALNSTP 180
             VKL+ELLV++TW EALPGEFEKPYA+NLCKFVE+EIC  G+PIYPPP LIFNALNST 
Sbjct: 121 GYVKLQELLVEDTWLEALPGEFEKPYAMNLCKFVESEICGGGIPIYPPPHLIFNALNSTS 180

Query: 181 FDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELREDLGCSIPSHGNLEK 240
           FDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSL+NIFKEL +DLGCSIPSHGNLEK
Sbjct: 181 FDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLVNIFKELEQDLGCSIPSHGNLEK 240

Query: 241 WAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQKKEGIIFLLWGNSAQAKLR 300
           WAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQ++EG++FLLWGNSAQ K+R
Sbjct: 241 WAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVIKTISQQREGVVFLLWGNSAQEKIR 300

Query: 301 LIDEKKHHILKAAHPSGLSANRGFFGCRHFSRTNVLLKELGTATIDWQL 319
           LID  KHHILKAAHPSGLSANRGFFGCRHFSRTN LLK++G  TIDWQL
Sbjct: 301 LIDTSKHHILKAAHPSGLSANRGFFGCRHFSRTNQLLKKMGIPTIDWQL 348

BLAST of Lsi11G001310 vs. NCBI nr
Match: gi|645228486|ref|XP_008221018.1| (PREDICTED: uracil-DNA glycosylase [Prunus mume])

HSP 1 Score: 454.9 bits (1169), Expect = 1.1e-124
Identity = 228/313 (72.84%), Postives = 255/313 (81.47%), Query Frame = 1

Query: 10  SKTRTLIDIFQPALS--KRLKTSQTLKTLATTDEKCDSELTLASSSMDMSAAQKSRMETN 69
           +KT+TL+D+FQP  S  KRLKT     T + +           SSS D++A QKSRME  
Sbjct: 60  NKTKTLLDLFQPTASSAKRLKTDSIRATHSDSVSPVPPPSHDDSSSSDLTAQQKSRMELQ 119

Query: 70  KWMAKSKRNLKMCSDRVSKWENGC--VKLEELLVDETWFEALPGEFEKPYALNLCKFVET 129
           K +AK++RNL +CS+R+S   +    VKLEELLV+ETW EA P E +KPYA  L KFVE 
Sbjct: 120 KLLAKARRNLSICSNRLSNSNSKGEGVKLEELLVEETWLEAFPSELQKPYAKTLSKFVEN 179

Query: 130 EICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSS 189
           EIC   +PIYPP  LIFNALNSTPFDRVK VILGQDPYHGPGQAMGLSFSVPEGVK+PSS
Sbjct: 180 EICGGALPIYPPTHLIFNALNSTPFDRVKAVILGQDPYHGPGQAMGLSFSVPEGVKVPSS 239

Query: 190 LLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQFTDAVI 249
           L+NIFKEL +DLGCSIPSHGNLEKWAVQGVLLLNAVLTVR HQANSHAKKGWEQFTDAVI
Sbjct: 240 LVNIFKELHQDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRNHQANSHAKKGWEQFTDAVI 299

Query: 250 KTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFSRTNVL 309
           KTISQK+EG++FLLWGNSAQ K +LIDE KHHILKAAHPSGLSANRGFFGCRHFSRTN L
Sbjct: 300 KTISQKREGVVFLLWGNSAQQKSKLIDESKHHILKAAHPSGLSANRGFFGCRHFSRTNQL 359

Query: 310 LKELGTATIDWQL 319
           L+E+G   IDWQL
Sbjct: 360 LEEMGIPPIDWQL 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
UNG_ARATH6.1e-11063.64Uracil-DNA glycosylase, mitochondrial OS=Arabidopsis thaliana GN=UNG PE=1 SV=1[more]
UNG_AZOVD1.3e-7258.04Uracil-DNA glycosylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=... [more]
UNG_PSEPK8.6e-7257.59Uracil-DNA glycosylase OS=Pseudomonas putida (strain KT2440) GN=ung PE=3 SV=1[more]
UNG_PSEP11.5e-7157.59Uracil-DNA glycosylase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ung PE... [more]
UNG_PSEPG2.5e-7158.04Uracil-DNA glycosylase OS=Pseudomonas putida (strain GB-1) GN=ung PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMG3_CUCSA7.4e-17193.71Uracil-DNA glycosylase OS=Cucumis sativus GN=Csa_5G289610 PE=3 SV=1[more]
W9RJ98_9ROSA1.0e-12470.82Uracil-DNA glycosylase OS=Morus notabilis GN=L484_009862 PE=3 SV=1[more]
M5XNR7_PRUPE4.0e-12472.52Uracil-DNA glycosylase OS=Prunus persica GN=PRUPE_ppa022483mg PE=3 SV=1[more]
A0A061E9U8_THECC2.2e-12271.61Uracil-DNA glycosylase OS=Theobroma cacao GN=TCM_011079 PE=3 SV=1[more]
A0A0B0MRI6_GOSAR7.0e-12172.73Uracil-DNA glycosylase OS=Gossypium arboreum GN=F383_28725 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G18630.13.4e-11163.64 uracil dna glycosylase[more]
Match NameE-valueIdentityDescription
gi|659130542|ref|XP_008465227.1|3.6e-17193.71PREDICTED: uracil-DNA glycosylase isoform X2 [Cucumis melo][more]
gi|449445338|ref|XP_004140430.1|1.1e-17093.71PREDICTED: uracil-DNA glycosylase isoform X1 [Cucumis sativus][more]
gi|659130540|ref|XP_008465225.1|5.4e-16786.88PREDICTED: uracil-DNA glycosylase isoform X1 [Cucumis melo][more]
gi|1009106111|ref|XP_015870160.1|1.2e-12669.34PREDICTED: uracil-DNA glycosylase, mitochondrial [Ziziphus jujuba][more]
gi|645228486|ref|XP_008221018.1|1.1e-12472.84PREDICTED: uracil-DNA glycosylase [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016799hydrolase activity, hydrolyzing N-glycosyl compounds
GO:0004844uracil DNA N-glycosylase activity
Vocabulary: Biological Process
TermDefinition
GO:0006284base-excision repair
GO:0006281DNA repair
Vocabulary: INTERPRO
TermDefinition
IPR018085Ura-DNA_Glyclase_AS
IPR005122Uracil-DNA_glycosylase-like
IPR002043UDG_fam1
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006284 base-excision repair
biological_process GO:0006281 DNA repair
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
cellular_component GO:0005575 cellular_component
molecular_function GO:0004844 uracil DNA N-glycosylase activity
molecular_function GO:0016799 hydrolase activity, hydrolyzing N-glycosyl compounds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G001310.1Lsi11G001310.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002043Uracil-DNA glycosylaseHAMAPMF_00148UDGcoord: 100..317
score: 38
IPR002043Uracil-DNA glycosylasePANTHERPTHR11264URACIL-DNA GLYCOSYLASEcoord: 13..318
score: 6.5E
IPR002043Uracil-DNA glycosylaseTIGRFAMsTIGR00628TIGR00628coord: 101..308
score: 1.2
IPR005122Uracil-DNA glycosylase-likeGENE3DG3DSA:3.40.470.10coord: 91..318
score: 2.0
IPR005122Uracil-DNA glycosylase-likePFAMPF03167UDGcoord: 151..307
score: 2.5
IPR005122Uracil-DNA glycosylase-likeSMARTSM00986UDG_2coord: 146..306
score: 5.7
IPR005122Uracil-DNA glycosylase-likeunknownSSF52141Uracil-DNA glycosylase-likecoord: 98..318
score: 2.83
IPR018085Uracil-DNA glycosylase, active sitePROSITEPS00130U_DNA_GLYCOSYLASEcoord: 154..163
scor
NoneNo IPR availablePANTHERPTHR11264:SF9SUBFAMILY NOT NAMEDcoord: 13..318
score: 6.5E
NoneNo IPR availableSMARTSM00987UDG_2_acoord: 146..306
score: 5.7

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi11G001310Cucurbita maxima (Rimu)cmalsiB635
Lsi11G001310Cucurbita moschata (Rifu)cmolsiB623