Lsi10G012050 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi10G012050
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPhospholipid:diacylglycerol acyltransferase 2
Locationchr10 : 16338364 .. 16342105 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTTCGTCAACCCTGTTAAGAGCTCTTCTATGGGGGTTCAACCCATTGAAGCACATAAATCAGAGGATAAACATAATGTCATTGTCGCATATGTGAAGAAATTGATGCAAATGAAGCAAGACAAGGACAAGAAGAAGAAAAGGAACAAGAACATCAAGAAGGGGCAATCAGGAGAATGGAAATGCTTAGATAACTGCTGTTGGATTATTGGATACATGTGTACATTTTGGTGGCTGATGTTTTTTCTCTACCATTATTTGCCTCTTACACTACCTGGCTTTCGGGCGATTGATTCTCCGGGAGCTGTTCTAAAGCGAGAAGGCATATCAGGTCTTCACCCAGTCGTTTTAGTGCCTGGAATTGTCACCGGCGGGCTGGAGCTCTGGGACGGCAAGCCCTGCGCCGAGGGCCTCTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGGTTAGCAAATTCCAGATCAATTTGACATTTCTGAAGCTTTTGAATTGATTTTGGAAGTACCCATTTGAAAATTTGAATTCAAATTTTCAGACCACTATGCTGGTTGGAGCACCTGTCTCTAGACAATGAAACAGGGCTTGACCCACCAGGCATTCGGGTCCGGCCAGTTGAAGGCCTTGTGGCTGCTGATTATTTCGCCCAAGGCTACTTCGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTGCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAATACAGAGGTATGTGACCCGAAACAGTCTTCTTCTTGCAATCATATATATATATCATCTCTTCATTGGGTTTGCCATAGTTGAACTGAGAAATCACGCAGGTTAGAGACAGAGCCCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGGAATAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTATTTCCTGCACTTCATGAAGTGGGTGGAAGCGCCGCCGCCGATGGGCGGCGGCGGCGGCGGCCTTGGGTGGTGTGCCAAGCATATTAAAGCAATCATGAATATTGGCTCTACATTCTTGGGAACTCCAAAGGCAGTCAGTAAAATTCTTTCTGCTGAAGACAGACATGTTGCTTTGCTCAGGTTCACTTCGATGTTCTCTTCTTTTCTTCACTCTAAATATTGTAAGTTTGTAATGTAACTATTATCTTTAAAGTATTTTATTTGTATTTAATGAATAAATTTTGTCGTTATTGTAATTTGTTATCAGTTTCCATTTCTCATTCTTATTTTCTTTTTTCACAGGTGTTTTGACAAAGTCTTTTAATCTTCGTTTCTCATTTTTCATATTCAATAGCAAATGGTTTAAAGTATTTTGGATTAAATGTTTTGTTTATAGAGTTTCAGAATTTATTGATAAACTTCATTTCAAATTTCACTAACTTTTCTGAACTAGAATTTTCAAGATAGAATTTATTTTTGAAATAAATGAGATGGATTTTTTTTTTTTTTTGGTATTTATTTCTCAAGAGGAAAAGAACTAAGAAATACAATACTTTTATAATTGTGTCAAAATTTTGACTGGAAGTGTAGGATTTCTCAGGCTTTGTGAATTATTAGAGAAAGGCATTTAGAATTTAGCCATCAAATATTTATTGAAGACAGAGAAAAAATTCTTAAGCAATGAAGAAGAGATTAACCTACAAATGACAGACAGAGAGAAGTCTTCTTATAGAGATTCAAGGACAAATAATGAACTTGGTTTTCTTGGCATTTTACAGAGCTATGACCCCTGGTTTTCTTAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATTTGTTGCGGGTTTCCCGAACGTGGGATTCGACCGCCTCCTTGTTGCCAAAAGGTGGTGAGACCATCTGGGGTGACTTAGATTGGTCTCCTGAAGATGTTCAGAATTGTGATTTGAAGAAAGTAGATACACATTCTTTTAGAAACGAAAATGATTCCAATGACAGTAAAAGAAAGAAAGCTTTCCAAGAAAAGGAGCCAGTGCACTATGGAAGAATAATCTCTTTTAGCAAAGAAGCAGCAACACGACCTTCTTCAGAGCTCTCTACACTAAATTCAGAGGTAATTTCTCGGCTACTTTCTGTGCATTGGAATTAAATTCTTTGATGGGGGCAAATGATGAAACTTAGCAGCGCCGAATGTGTTAGATACTACTTTTTTTTTTTTTTTTTTTTGATAGGTGAGCTTAGATATTCAAAGTGGGACATTTTTCTCTATATCTTGAGCGTAGGATAGTATTCACTAATGAGTGGATCTTGAATAGGAACTTAAAAATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGATGTATGGACTGAATATGATGAGATGAATAGGGAAAGCATTCGAAAAGTCTCTGTGAATAAAGCCTACACAGTGAAAACTGTTTTCGATCTACTCCGAGTTGTGGCTCCAAAAATGATGCAGCGAATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATTATAAGTACTGGTCAAATCCACTTGAGACAAAGTAAGTACCTGCGTAGATTTTTAGAATTTTCTGATTTTCTTTAGAAGATAATCTTCCACAATGAAGTACTGTCAACTCTCGGCCAGCTGATAATCCAATAACTATCTAACATCAAGAACATGGTTTTGGAGGTATTATTTGCTAATTTCAAATGCAATCAAACAACTTCCCTCAATCACTGTCCCCAAAAACAATACACACATTTCTCAACTTTCCGTTTCATTACTTCTGTCTTGCGGCCAGTCCTGAAACTAAATACATTGTTGAAAATCCGTTTCAGACTACCAGATGCCCCAGATATGGAGATATACTGCTTATATGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATTGAGGGAAAAGGGAGTGGCTGTCTGAGAAGCGGTATGTACTTTGTCGACGGTGACGACTCTGTACCGGTAGTGAGCTCCGGCTTCATGTGTGCAAAAGGGTGGCGAAACAAAACACGATTCAACCCATCAGGTAGCCCTACATACGTAAGAGAGTACAGGCACAAGGCTCCTGCAAGTCTCCTAGAAGGAAGAGGAGTTGAAAGTAGTTCTCACGTTGATATAATGGGAAATGTTAATCTTATTGAAGATATCTTGAGGGTTGCGGCTGGAGCCACTGGAGCAGAGATTGGTGGTGACAAGATTTACTCAGATATATTAACACTATCAGAGAGAATAAATCTTAAGCTGTGATTCGGTTTGAAAGCTCAAGAGATGGAGTTGAAAGGAAAATTAACGGGCTTCTAGAGCAGGATAGCATAAAATGGAGGGTATAGAAGAGAGGAAAATAATTCATACTCTTGGCAATGGAGTTTGTTCCCCCGGGTTTTTTCATGTGAATAATATTGTGATGTTTGAGGTCTACTTTCTAAACCCGATCATTGTCCTTCCAACATGTCCATTTTCGGTGCCTCTACTTTCTAAAATATCATACAATCTTCTGAAACATAACTTGCCACAGTGTAACCTAGGAGTTACGAATATGATATTGTCTATTTGTCAAATTCAGACTAAAAAATGTAGGTGGGTTTTGCGATTTGGTAGCAGG

mRNA sequence

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTTCGTCAACCCTGTTAAGAGCTCTTCTATGGGGGTTCAACCCATTGAAGCACATAAATCAGAGGATAAACATAATGTCATTGTCGCATATGTGAAGAAATTGATGCAAATGAAGCAAGACAAGGACAAGAAGAAGAAAAGGAACAAGAACATCAAGAAGGGGCAATCAGGAGAATGGAAATGCTTAGATAACTGCTGTTGGATTATTGGATACATGTGTACATTTTGGTGGCTGATGTTTTTTCTCTACCATTATTTGCCTCTTACACTACCTGGCTTTCGGGCGATTGATTCTCCGGGAGCTGTTCTAAAGCGAGAAGGCATATCAGGTCTTCACCCAGTCGTTTTAGTGCCTGGAATTGTCACCGGCGGGCTGGAGCTCTGGGACGGCAAGCCCTGCGCCGAGGGCCTCTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCACTATGCTGGTTGGAGCACCTGTCTCTAGACAATGAAACAGGGCTTGACCCACCAGGCATTCGGGTCCGGCCAGTTGAAGGCCTTGTGGCTGCTGATTATTTCGCCCAAGGCTACTTCGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTGCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAATACAGAGGTTAGAGACAGAGCCCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGGAATAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTATTTCCTGCACTTCATGAAGTGGGTGGAAGCGCCGCCGCCGATGGGCGGCGGCGGCGGCGGCCTTGGGTGGTGTGCCAAGCATATTAAAGCAATCATGAATATTGGCTCTACATTCTTGGGAACTCCAAAGGCAGTCAGTAAAATTCTTTCTGCTGAAGACAGACATGTTGCTTTGCTCAGAGCTATGACCCCTGGTTTTCTTAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATTTGTTGCGGGTTTCCCGAACGTGGGATTCGACCGCCTCCTTGTTGCCAAAAGGTGGTGAGACCATCTGGGGTGACTTAGATTGGTCTCCTGAAGATGTTCAGAATTGTGATTTGAAGAAAGTAGATACACATTCTTTTAGAAACGAAAATGATTCCAATGACAGTAAAAGAAAGAAAGCTTTCCAAGAAAAGGAGCCAGTGCACTATGGAAGAATAATCTCTTTTAGCAAAGAAGCAGCAACACGACCTTCTTCAGAGCTCTCTACACTAAATTCAGAGGAACTTAAAAATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGATGTATGGACTGAATATGATGAGATGAATAGGGAAAGCATTCGAAAAGTCTCTGTGAATAAAGCCTACACAGTGAAAACTGTTTTCGATCTACTCCGAGTTGTGGCTCCAAAAATGATGCAGCGAATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATTATAAGTACTGGTCAAATCCACTTGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATATGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATTGAGGGAAAAGGGAGTGGCTGTCTGAGAAGCGGTATGTACTTTGTCGACGGTGACGACTCTGTACCGGTAGTGAGCTCCGGCTTCATGTGTGCAAAAGGGTGGCGAAACAAAACACGATTCAACCCATCAGGTAGCCCTACATACGTAAGAGAGTACAGGCACAAGGCTCCTGCAAGTCTCCTAGAAGGAAGAGGAGTTGAAAGTAGTTCTCACGTTGATATAATGGGAAATGTTAATCTTATTGAAGATATCTTGAGGGTTGCGGCTGGAGCCACTGGAGCAGAGATTGGTGGTGACAAGATTTACTCAGATATATTAACACTATCAGAGAGAATAAATCTTAAGCTGTGATTCGGTTTGAAAGCTCAAGAGATGGAGTTGAAAGGAAAATTAACGGGCTTCTAGAGCAGGATAGCATAAAATGGAGGGTATAGAAGAGAGGAAAATAATTCATACTCTTGGCAATGGAGTTTGTTCCCCCGGGTTTTTTCATGTGAATAATATTGTGATGTTTGAGGTCTACTTTCTAAACCCGATCATTGTCCTTCCAACATGTCCATTTTCGGTGCCTCTACTTTCTAAAATATCATACAATCTTCTGAAACATAACTTGCCACAGTGTAACCTAGGAGTTACGAATATGATATTGTCTATTTGTCAAATTCAGACTAAAAAATGTAGGTGGGTTTTGCGATTTGGTAGCAGG

Coding sequence (CDS)

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTTCGTCAACCCTGTTAAGAGCTCTTCTATGGGGGTTCAACCCATTGAAGCACATAAATCAGAGGATAAACATAATGTCATTGTCGCATATGTGAAGAAATTGATGCAAATGAAGCAAGACAAGGACAAGAAGAAGAAAAGGAACAAGAACATCAAGAAGGGGCAATCAGGAGAATGGAAATGCTTAGATAACTGCTGTTGGATTATTGGATACATGTGTACATTTTGGTGGCTGATGTTTTTTCTCTACCATTATTTGCCTCTTACACTACCTGGCTTTCGGGCGATTGATTCTCCGGGAGCTGTTCTAAAGCGAGAAGGCATATCAGGTCTTCACCCAGTCGTTTTAGTGCCTGGAATTGTCACCGGCGGGCTGGAGCTCTGGGACGGCAAGCCCTGCGCCGAGGGCCTCTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCACTATGCTGGTTGGAGCACCTGTCTCTAGACAATGAAACAGGGCTTGACCCACCAGGCATTCGGGTCCGGCCAGTTGAAGGCCTTGTGGCTGCTGATTATTTCGCCCAAGGCTACTTCGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTGCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAATACAGAGGTTAGAGACAGAGCCCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGGAATAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTATTTCCTGCACTTCATGAAGTGGGTGGAAGCGCCGCCGCCGATGGGCGGCGGCGGCGGCGGCCTTGGGTGGTGTGCCAAGCATATTAAAGCAATCATGAATATTGGCTCTACATTCTTGGGAACTCCAAAGGCAGTCAGTAAAATTCTTTCTGCTGAAGACAGACATGTTGCTTTGCTCAGAGCTATGACCCCTGGTTTTCTTAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATTTGTTGCGGGTTTCCCGAACGTGGGATTCGACCGCCTCCTTGTTGCCAAAAGGTGGTGAGACCATCTGGGGTGACTTAGATTGGTCTCCTGAAGATGTTCAGAATTGTGATTTGAAGAAAGTAGATACACATTCTTTTAGAAACGAAAATGATTCCAATGACAGTAAAAGAAAGAAAGCTTTCCAAGAAAAGGAGCCAGTGCACTATGGAAGAATAATCTCTTTTAGCAAAGAAGCAGCAACACGACCTTCTTCAGAGCTCTCTACACTAAATTCAGAGGAACTTAAAAATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGATGTATGGACTGAATATGATGAGATGAATAGGGAAAGCATTCGAAAAGTCTCTGTGAATAAAGCCTACACAGTGAAAACTGTTTTCGATCTACTCCGAGTTGTGGCTCCAAAAATGATGCAGCGAATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATTATAAGTACTGGTCAAATCCACTTGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATATGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATTGAGGGAAAAGGGAGTGGCTGTCTGAGAAGCGGTATGTACTTTGTCGACGGTGACGACTCTGTACCGGTAGTGAGCTCCGGCTTCATGTGTGCAAAAGGGTGGCGAAACAAAACACGATTCAACCCATCAGGTAGCCCTACATACGTAAGAGAGTACAGGCACAAGGCTCCTGCAAGTCTCCTAGAAGGAAGAGGAGTTGAAAGTAGTTCTCACGTTGATATAATGGGAAATGTTAATCTTATTGAAGATATCTTGAGGGTTGCGGCTGGAGCCACTGGAGCAGAGATTGGTGGTGACAAGATTTACTCAGATATATTAACACTATCAGAGAGAATAAATCTTAAGCTGTGA

Protein sequence

MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL
BLAST of Lsi10G012050 vs. Swiss-Prot
Match: PDAT2_ARATH (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 2.3e-255
Identity = 433/662 (65.41%), Postives = 520/662 (78.55%), Query Frame = 1

Query: 43  KKLMQMKQD----KDKKKKRNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLP 102
           +KL    +D    K K+    +  K+ +SG   C+D+CCW+IGY+CT WWL+ FLYH +P
Sbjct: 8   RKLSSFSEDTINPKPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVP 67

Query: 103 LTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSF 162
           +     +A +SPG  L R+G+   HPV+LVPGIVTGGLELW+G+PCAEGLFRKRLWG SF
Sbjct: 68  VPAM-LQAPESPGTRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASF 127

Query: 163 TETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGY 222
           +E L+RPLCWLEHLSLD+ETGLDP GIRVR V GLVAADYFA  YF WAVLIENLAKIGY
Sbjct: 128 SEILRRPLCWLEHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGY 187

Query: 223 DGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHF 282
           +GKNLHMA+YDWR++F NTEVRD++LSRLKSKIE+MY TNG  KVVVVPHSMG +YFLHF
Sbjct: 188 EGKNLHMASYDWRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHF 247

Query: 283 MKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTP 342
           +KWVE P P GGGGGG GWCAKHIK+++NIG  FLG PKAVS +LSAE + +A  R++ P
Sbjct: 248 LKWVETPLPDGGGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAP 307

Query: 343 GFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNC--DLKKVDT 402
           G L SE+L  QTLEHL+R+S +WDS  SLLPKGGE IWGDLD   E+  NC    +K   
Sbjct: 308 GLLDSELLKLQTLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKSSQ 367

Query: 403 HSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKN-SGSSN 462
            S  N +  N S  K   + KEP  YGRI+SF K A+  PSS+LSTLN +EL    G+SN
Sbjct: 368 LSLSNLHKQNYS-LKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSN 427

Query: 463 DSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAE 522
           DS+ SC + W+EY+EM+RESI KV+ N AYT  TV DLLR +APKMM+R ++HFSHGIA+
Sbjct: 428 DST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIAD 487

Query: 523 DLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK-SIPF 582
           DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK SIPF
Sbjct: 488 DLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPF 547

Query: 583 RIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRH 642
           RID S++G    CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++REY+H
Sbjct: 548 RIDGSLDG-DDVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKH 607

Query: 643 KAPASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINL 695
           K P SLLE RG ES +HVDIMGNV LIED+LR+AAGA+G EIGGD+IYSD++ +SERI++
Sbjct: 608 KPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISI 665

BLAST of Lsi10G012050 vs. Swiss-Prot
Match: PDAT1_ARATH (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 7.8e-227
Identity = 383/661 (57.94%), Postives = 478/661 (72.31%), Query Frame = 1

Query: 47  QMKQDKDKKKK-----RNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTL 106
           ++  D+D +KK     ++ + K    G+W C+D+CCW IG +C  WW + FLY+ +P + 
Sbjct: 20  EVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASF 79

Query: 107 PGFRAI-------DSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLW 166
           P +          D PG  LK+EG+   HPVV +PGIVTGGLELW+GK CA+GLFRKRLW
Sbjct: 80  PQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLW 139

Query: 167 GGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLA 226
           GG+F E  KRPLCW+EH+SLDNETGLDP GIRVR V GLVAADYFA GYFVWAVLI NLA
Sbjct: 140 GGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLA 199

Query: 227 KIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLY 286
            IGY+ KN++MAAYDWR++FQNTEVRD+ LSR+KS IE+M  TNGG K V+VPHSMGVLY
Sbjct: 200 HIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLY 259

Query: 287 FLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLR 346
           FLHFMKWVEAP P+GGGGG   WCAK+IKA+MNIG  FLG PKAV+ + SAE + VA+ R
Sbjct: 260 FLHFMKWVEAPAPLGGGGGP-DWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVAR 319

Query: 347 AMTPGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKV 406
           A+ PGFL ++I   QTL+H++R++RTWDST S+LPKGG+TIWG LDWSPE    C  KK 
Sbjct: 320 AIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKK- 379

Query: 407 DTHSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSS 466
                +  N++     +    +K PV+YGR+ISF KE A    SE++ ++        S 
Sbjct: 380 -----QKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSI 439

Query: 467 NDSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIA 526
              + +C DVWTEY +M    I+ ++  K YT     DLL  VAPKMM R  +HFS+GIA
Sbjct: 440 --PNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIA 499

Query: 527 EDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRI 586
           +DLDD KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I
Sbjct: 500 DDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQI 559

Query: 587 DLSI-EGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 646
             S  E     CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H 
Sbjct: 560 FTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHS 619

Query: 647 APASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLK 695
            PA+LLEGRG +S +HVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LK
Sbjct: 620 PPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLK 671

BLAST of Lsi10G012050 vs. Swiss-Prot
Match: PDAT_SCHPO (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=plh1 PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 3.7e-59
Identity = 119/298 (39.93%), Postives = 169/298 (56.71%), Query Frame = 1

Query: 111 GAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGG--SFTETLKRPLCW 170
           G  +  EG    HPV++VPG+++ GLE W    C+   FRKRLWG             CW
Sbjct: 134 GLDMYNEGYRSDHPVIMVPGVISSGLESWSFNNCSIPYFRKRLWGSWSMLKAMFLDKQCW 193

Query: 171 LEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAY 230
           LEHL LD +TGLDP GI++R  +G  AAD+F  GY++W+ +IENLA IGY+  N+  A+Y
Sbjct: 194 LEHLMLDKKTGLDPKGIKLRAAQGFEAADFFITGYWIWSKVIENLAAIGYEPNNMLSASY 253

Query: 231 DWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM 290
           DWR+++ N E RD+  S+LK  IE     +   KVV++ HSMG     +F KWVEA    
Sbjct: 254 DWRLSYANLEERDKYFSKLKMFIEYSNIVH-KKKVVLISHSMGSQVTYYFFKWVEAE--- 313

Query: 291 GGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGF 350
           G G GG  W   HI+A +NI  + +G PK V+ +LS E +  A L   +   L+     F
Sbjct: 314 GYGNGGPTWVNDHIEAFINISGSLIGAPKTVAALLSGEMKDTAQLNQFSVYGLEK---FF 373

Query: 351 QTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSN 407
              E  + V RT    +S+LPKGG+ +WG+  W+P+D+   +        +R + D +
Sbjct: 374 SRSERAMMV-RTMGGVSSMLPKGGDVVWGNASWAPDDLNQTNFSNGAIIRYREDIDKD 423

BLAST of Lsi10G012050 vs. Swiss-Prot
Match: PDAT_YEAST (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LRO1 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.9e-57
Identity = 119/307 (38.76%), Postives = 176/307 (57.33%), Query Frame = 1

Query: 113 VLKREGISGLHPVVLVPGIVTGGLELW----DGKPCAEGLFRKRLWGGSFT--ETLKRPL 172
           +L+   I   HPVV+VPG+++ G+E W    D +  +   FRKRLWG  +     +   +
Sbjct: 163 LLRDYNIEAKHPVVMVPGVISTGIESWGVIGDDECDSSAHFRKRLWGSFYMLRTMVMDKV 222

Query: 173 CWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMA 232
           CWL+H+ LD ETGLDPP   +R  +G  + DYF  GY++W  + +NL  IGY+   +  A
Sbjct: 223 CWLKHVMLDPETGLDPPNFTLRAAQGFESTDYFIAGYWIWNKVFQNLGVIGYEPNKMTSA 282

Query: 233 AYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPP 292
           AYDWR+A+ + E RDR  ++LK +IE+ ++ + G KV ++ HSMG     +FMKWVEA  
Sbjct: 283 AYDWRLAYLDLERRDRYFTKLKEQIELFHQLS-GEKVCLIGHSMGSQIIFYFMKWVEAEG 342

Query: 293 PMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEIL 352
           P+  G GG GW  +HI + +N   T LG PKAV  ++S E +    L  +    L+    
Sbjct: 343 PL-YGNGGRGWVNEHIDSFINAAGTLLGAPKAVPALISGEMKDTIQLNTLAMYGLEK--- 402

Query: 353 GFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHS--FRNEND 412
            F  +E  +++ +TW    S+LPKG E IWGD+  S ED  N      DT+    R E +
Sbjct: 403 FFSRIER-VKMLQTWGGIPSMLPKGEEVIWGDMKSSSEDALN---NNTDTYGNFIRFERN 460

BLAST of Lsi10G012050 vs. Swiss-Prot
Match: LCAT2_ARATH (Phospholipid--sterol O-acyltransferase OS=Arabidopsis thaliana GN=PSAT PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 6.2e-14
Identity = 57/212 (26.89%), Postives = 87/212 (41.04%), Query Frame = 1

Query: 126 VLVPGIVTGGLELWDGKPCAEGLFRKR----LWGGSFTETLKRPLCWLEHLSLDNETGLD 185
           +++PG  +  L  W    C            +W  + T+ L    CW + + LD     D
Sbjct: 44  IIIPGFASTQLRAWSILDCPYTPLDFNPLDLVWLDT-TKLLSAVNCWFKCMVLDPYNQTD 103

Query: 186 PPGIRVRPVEGLVAADYFAQGYF------VWAVLIENLAKIGYDGKNLHMAAYDWRIAFQ 245
            P  + RP  GL A      GY       VW   ++   + G +   +    YDWR++  
Sbjct: 104 HPECKSRPDSGLSAITELDPGYITGPLSTVWKEWLKWCVEFGIEANAIVAVPYDWRLSPT 163

Query: 246 NTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVE---APPPMGGGG 305
             E RD    +LK   E   +  GG  +V   HSMG   F +F++W+    AP       
Sbjct: 164 KLEERDLYFHKLKLTFETALKLRGGPSIVFA-HSMGNNVFRYFLEWLRLEIAPKHY---- 223

Query: 306 GGLGWCAKHIKAIMNIGSTFLGTPKAVSKILS 325
             L W  +HI A   +G+  LG+ +A+   LS
Sbjct: 224 --LKWLDQHIHAYFAVGAPLLGSVEAIKSTLS 247

BLAST of Lsi10G012050 vs. TrEMBL
Match: A0A0A0KGM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149960 PE=4 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 672/694 (96.83%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKS++K NVIVAYVK LMQ KQDKD KKKRNK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           NI +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL+LPGF  IDSPGAVLKREGIS
Sbjct: 61  NINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGG LGWCAK
Sbjct: 241 DRALSRLKSKIEVMYATNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGG-LGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELST N EELKNSG SNDSSFSCSDVWTEYDEM+RESIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTQNLEELKNSGGSNDSSFSCSDVWTEYDEMSRESIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTV+TVFDLLR+VAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVETVFDLLRIVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           VVSSGFMCAKGWRNKTRFNPSGSPT+VREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 VVSSGFMCAKGWRNKTRFNPSGSPTHVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of Lsi10G012050 vs. TrEMBL
Match: F6GW64_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0061g01110 PE=4 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 1.3e-284
Identity = 479/697 (68.72%), Postives = 560/697 (80.34%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MAS+LR RKL +V PVK SS+G +  E  K E K +     VK +     +K KKK    
Sbjct: 1   MASILRFRKLCYVEPVKCSSLGFESFETPKIEKKDDR--GAVKAVESTVGEKQKKKS--- 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
              K Q  EW+C+D CC  +GY+CT WWL+ FLYH LP  LPGF+  ++PGA L+REG++
Sbjct: 61  --SKRQPREWRCMDYCCLTVGYVCTAWWLLLFLYHCLPAALPGFQVPEAPGARLRREGLT 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
            LHPVVLVPGIVTGGLELW+G+PC+EGLFRKRLWGGSFTE  KRPLCWLEHL+L N+TGL
Sbjct: 121 ALHPVVLVPGIVTGGLELWEGRPCSEGLFRKRLWGGSFTEIFKRPLCWLEHLTLHNQTGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVR V GLVAADYFA GYFVWAVLIENLA+IGY+GKNL+MAAYDWR++FQNTE+R
Sbjct: 181 DPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLARIGYEGKNLYMAAYDWRLSFQNTEIR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           D+ALSRLKSKIE+M+ TNG  KVVVVPHSMGV+YF HF+KWVE+PPPM GGGGG GWCAK
Sbjct: 241 DQALSRLKSKIELMFVTNGNKKVVVVPHSMGVIYFFHFLKWVESPPPM-GGGGGTGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIG  FLG PKAVS I SAE + VA +R+M PG L SEILG QTLEH++RV RT
Sbjct: 301 HIKAIMNIGPAFLGVPKAVSNIFSAEAKDVAFVRSMDPGVLDSEILGLQTLEHVMRVCRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDL--KKVDTHSFRNENDSNDSKRKKAFQEKE 420
           WDS  SL+PKGGETIWG+LDWSPE+  NCDL  K+    S  + ND+N S  K  FQ KE
Sbjct: 361 WDSIISLIPKGGETIWGNLDWSPEEGYNCDLAKKRYAQPSLLDSNDTN-SDGKMEFQIKE 420

Query: 421 PVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRK 480
            V YGRIISF K  +  PSS+L  L S++   + +    +  CS+VWTEYDEM+R+ IRK
Sbjct: 421 SVRYGRIISFGKATSQLPSSQLPNLGSKDFLRTSNPTKFNSLCSEVWTEYDEMSRDGIRK 480

Query: 481 VSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPD 540
           ++ NKAYT +T  DLLR VAPKMMQR ++ FSHGIA++LDDPKYAHYKYWSNPLETKLPD
Sbjct: 481 IAENKAYTARTFLDLLRFVAPKMMQRAEAQFSHGIADNLDDPKYAHYKYWSNPLETKLPD 540

Query: 541 APDMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDD 600
           APDMEIYCLYGVG+PTERSY+Y  S  D+CKSIPFRID S EG   GCL+ G+YFVDGD+
Sbjct: 541 APDMEIYCLYGVGLPTERSYVYKLSPTDRCKSIPFRIDSSAEGSDGGCLKGGVYFVDGDE 600

Query: 601 SVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVN 660
           SVPV+S+GFMCAKGW+ +TRFNPSG  TYVREYRHK PASL EGRGVES +HVDIMGNV 
Sbjct: 601 SVPVLSAGFMCAKGWKGRTRFNPSGIATYVREYRHKPPASLFEGRGVESGAHVDIMGNVA 660

Query: 661 LIEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           LIED+LRVAAGATG E+GGD+IYSDI+ +S+RINL+L
Sbjct: 661 LIEDVLRVAAGATGVEMGGDRIYSDIMRMSDRINLRL 688

BLAST of Lsi10G012050 vs. TrEMBL
Match: M5W8A4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015987mg PE=4 SV=1)

HSP 1 Score: 979.5 bits (2531), Expect = 2.0e-282
Identity = 474/696 (68.10%), Postives = 561/696 (80.60%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDK-HNVIVAYVKKLMQMKQDKDKKKKRN 60
           MAS+LR RKL +V P   SS+  Q  E  K E++    + +  + L++ KQ K+K KK++
Sbjct: 1   MASILRFRKLCYVEPC--SSLKFQSFETPKIENRDEGGVGSKTQNLLEKKQRKNKNKKQH 60

Query: 61  KNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGI 120
           +        EW+C+D+CCW+IGYMCT  W++ FLYH LP TL GF   +SPG  LK EG+
Sbjct: 61  R--------EWRCIDSCCWVIGYMCTTCWVLLFLYHSLPATLTGFPVPESPGTRLKAEGL 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           + LHPVVLVPGIVTGGLELW+G+PCAEGLFRKRLWGG FTE  KRPLCWLEHLSL NETG
Sbjct: 121 TPLHPVVLVPGIVTGGLELWEGRPCAEGLFRKRLWGGGFTEIFKRPLCWLEHLSLHNETG 180

Query: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRVR V GLVAADYFA GYFVWAVLIENLAKIGY+GKN+HMAAYDWR++FQNTE+
Sbjct: 181 LDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEGKNMHMAAYDWRLSFQNTEI 240

Query: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCA 300
           RD+AL+RLKSKIE+MY TNG  KVVVVPHSMGV+YFLHFMKWVE+PPPMGGGGG   WCA
Sbjct: 241 RDQALTRLKSKIELMYVTNGHKKVVVVPHSMGVIYFLHFMKWVESPPPMGGGGGP-SWCA 300

Query: 301 KHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSR 360
           KHIKAIMNIG  FLG PKAVS I SAE + VA +RAM PG L SEIL  QTLEH++R SR
Sbjct: 301 KHIKAIMNIGPAFLGVPKAVSNIFSAEGKDVAYIRAMDPGVLDSEILRLQTLEHVMRASR 360

Query: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEP 420
           TWDS  SLLPKGG+ IWG+LDWSPED  +CDL K        + + N+    + FQ KEP
Sbjct: 361 TWDSIVSLLPKGGDAIWGNLDWSPEDGHSCDLAKKGYLQTSGDTNFNNRDENRVFQVKEP 420

Query: 421 VHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKV 480
           V YGRIISF KE +   S +L   + ++L +  S+ + + SC +VWTEYDE++RESIRK+
Sbjct: 421 VKYGRIISFGKETSQISSPQLPRHDFKDLPHKDSATNFTSSCGEVWTEYDELSRESIRKI 480

Query: 481 SVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA 540
           + NKAYT +T+FDLLR VAPKMMQR ++HFSHGIA++LDDPKYAHYKYWSNPLETKLP A
Sbjct: 481 TENKAYTAQTLFDLLRFVAPKMMQRAEAHFSHGIADNLDDPKYAHYKYWSNPLETKLPVA 540

Query: 541 PDMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDS 600
           PDMEIY LYGVGIPTERSY+Y  S  DKCKSIPFRID S EG+   CL++G+YFVDGDDS
Sbjct: 541 PDMEIYSLYGVGIPTERSYVYKMSPSDKCKSIPFRIDGSAEGEAGSCLKNGVYFVDGDDS 600

Query: 601 VPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNL 660
           VPV+S+G MCAKGWR +TRFNPSG  TY+REY+HK PASLLEGRG+ES +HVDIMGNV L
Sbjct: 601 VPVLSAGLMCAKGWRGRTRFNPSGIATYIREYQHKPPASLLEGRGIESGAHVDIMGNVAL 660

Query: 661 IEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           IED+LR++AGATGA++GGD+IYSDI+ +SERINL+L
Sbjct: 661 IEDVLRISAGATGAQVGGDRIYSDIMRMSERINLQL 685

BLAST of Lsi10G012050 vs. TrEMBL
Match: A0A067DI02_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005610mg PE=4 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 2.7e-282
Identity = 472/695 (67.91%), Postives = 555/695 (79.86%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MAS+LR RKL +  P+K  S     ++  +  DK             + +  D+KKK  K
Sbjct: 1   MASILRFRKLCYAVPLKLQSFEFSKVDQKQKVDKKEEDANVFLPAQDLLEVDDQKKKYPK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
                Q  +W+C+D+CCW IGY+CT WWL+FFLYH  P T+PGF+  +SPG  LKR+G++
Sbjct: 61  Q----QQRKWRCIDSCCWAIGYLCTTWWLLFFLYHCFPATMPGFQVPESPGVRLKRDGLT 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
            LHPVVLVPGIVTGGLELW+G+PC+EGLFRKRLWGGSFTE  KRPLCWLEHLSL +ETGL
Sbjct: 121 ALHPVVLVPGIVTGGLELWEGRPCSEGLFRKRLWGGSFTEIFKRPLCWLEHLSLHHETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVR V GLVAADYFA GYFVWAVLIENLAKIGY+GKNL+MA+YDWR++FQNTE+R
Sbjct: 181 DPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEGKNLYMASYDWRLSFQNTEIR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           D+ALSRLKSKIE++  TNG  KVVVVPHSMGV+YFLHF+KWVE PPPMGGGGG  GWCAK
Sbjct: 241 DQALSRLKSKIELLCVTNGYKKVVVVPHSMGVIYFLHFLKWVETPPPMGGGGGP-GWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+++NIG  FLG PKAVS I SAE + VA LRAM PG L SEILG QTLEH+LRVSRT
Sbjct: 301 HIKSVVNIGPAFLGVPKAVSSIFSAEGKDVAYLRAMAPGLLDSEILGLQTLEHVLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDS  SLLPKGGETIWGDLDWSPE+   C L  V   +F+   + N +   + FQ KE  
Sbjct: 361 WDSVVSLLPKGGETIWGDLDWSPEEGHACQL--VKKGNFQCSPNDNYTDAMRGFQIKETE 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
            YGRIISF KE +  PSS++  LNS+EL +  ++   + SC  VWTEYDEM+RES RK++
Sbjct: 421 KYGRIISFGKEESQLPSSQIPILNSKELLHGSATETVNSSCRGVWTEYDEMDRESFRKIA 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NK YT KT+ DLLR VAPK MQR ++HFSHGIA++LDDPKY HYKYWSNPLETKLPDA 
Sbjct: 481 ENKVYTSKTILDLLRFVAPKTMQRAETHFSHGIADNLDDPKYEHYKYWSNPLETKLPDAA 540

Query: 541 DMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSV 600
           DMEIYCLYGVGIPTERSY+Y  S  DKCKSIPFRID S +G  + CL+ G+YFVDGD+SV
Sbjct: 541 DMEIYCLYGVGIPTERSYVYKLSPNDKCKSIPFRIDTSADGDQNSCLKGGVYFVDGDESV 600

Query: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLI 660
           PV+S+GFMCAKGWR +TRFNPSG+ TY+REY+HK PASLLEGRG+ES +HVDIMGNV LI
Sbjct: 601 PVLSAGFMCAKGWRGRTRFNPSGTATYIREYQHKPPASLLEGRGLESGAHVDIMGNVALI 660

Query: 661 EDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           ED+LRVAAGA+G+EIGGD+IYSDIL +SERINL+L
Sbjct: 661 EDVLRVAAGASGSEIGGDRIYSDILRMSERINLRL 688

BLAST of Lsi10G012050 vs. TrEMBL
Match: V4SFM5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027727mg PE=4 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 2.7e-282
Identity = 472/695 (67.91%), Postives = 555/695 (79.86%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MAS+LR RKL +  P+K  S     ++  +  DK             + +  D+KKK  K
Sbjct: 1   MASILRFRKLCYAVPLKLQSFEFSKVDQKQKVDKKEEDANVFLPAQDLLEVDDQKKKYPK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
                Q  +W+C+D+CCW IGY+CT WWL+FFLYH  P T+PGF+  +SPG  LKR+G++
Sbjct: 61  Q----QQRKWRCIDSCCWAIGYLCTTWWLLFFLYHCFPATMPGFQVPESPGVRLKRDGLT 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
            LHPVVLVPGIVTGGLELW+G+PC+EGLFRKRLWGGSFTE  KRPLCWLEHLSL +ETGL
Sbjct: 121 ALHPVVLVPGIVTGGLELWEGRPCSEGLFRKRLWGGSFTEIFKRPLCWLEHLSLHHETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVR V GLVAADYFA GYFVWAVLIENLAKIGY+GKNL+MA+YDWR++FQNTE+R
Sbjct: 181 DPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEGKNLYMASYDWRLSFQNTEIR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           D+ALSRLKSKIE++  TNG  KVVVVPHSMGV+YFLHF+KWVE PPPMGGGGG  GWCAK
Sbjct: 241 DQALSRLKSKIELLCVTNGYKKVVVVPHSMGVIYFLHFLKWVETPPPMGGGGGP-GWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+++NIG  FLG PKAVS I SAE + VA LRAM PG L SEILG QTLEH+LRVSRT
Sbjct: 301 HIKSVVNIGPAFLGVPKAVSSIFSAEGKDVAYLRAMAPGLLDSEILGLQTLEHVLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDS  SLLPKGGETIWGDLDWSPE+   C L  V   +F+   + N +   + FQ KE  
Sbjct: 361 WDSVVSLLPKGGETIWGDLDWSPEEGHACQL--VKKGNFQCSPNDNYTDAMRGFQIKETE 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
            YGRIISF KE +  PSS++  LNS+EL +  ++   + SC  VWTEYDEM+RES RK++
Sbjct: 421 KYGRIISFGKEESQLPSSQIPILNSKELLHGSATETVNSSCRGVWTEYDEMDRESFRKIA 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NK YT KT+ DLLR VAPK MQR ++HFSHGIA++LDDPKY HYKYWSNPLETKLPDA 
Sbjct: 481 ENKVYTSKTILDLLRFVAPKTMQRAETHFSHGIADNLDDPKYEHYKYWSNPLETKLPDAA 540

Query: 541 DMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSV 600
           DMEIYCLYGVGIPTERSY+Y  S  DKCKSIPFRID S +G  + CL+ G+YFVDGD+SV
Sbjct: 541 DMEIYCLYGVGIPTERSYVYKLSPNDKCKSIPFRIDTSADGDQNSCLKGGVYFVDGDESV 600

Query: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLI 660
           PV+S+GFMCAKGWR +TRFNPSG+ TY+REY+HK PASLLEGRG+ES +HVDIMGNV LI
Sbjct: 601 PVLSAGFMCAKGWRGRTRFNPSGTATYIREYQHKPPASLLEGRGLESGAHVDIMGNVALI 660

Query: 661 EDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           ED+LRVAAGA+G+EIGGD+IYSDIL +SERINL+L
Sbjct: 661 EDVLRVAAGASGSEIGGDRIYSDILRMSERINLRL 688

BLAST of Lsi10G012050 vs. TAIR10
Match: AT3G44830.1 (AT3G44830.1 Lecithin:cholesterol acyltransferase family protein)

HSP 1 Score: 882.9 bits (2280), Expect = 1.3e-256
Identity = 433/662 (65.41%), Postives = 520/662 (78.55%), Query Frame = 1

Query: 43  KKLMQMKQD----KDKKKKRNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLP 102
           +KL    +D    K K+    +  K+ +SG   C+D+CCW+IGY+CT WWL+ FLYH +P
Sbjct: 8   RKLSSFSEDTINPKPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVP 67

Query: 103 LTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSF 162
           +     +A +SPG  L R+G+   HPV+LVPGIVTGGLELW+G+PCAEGLFRKRLWG SF
Sbjct: 68  VPAM-LQAPESPGTRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASF 127

Query: 163 TETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGY 222
           +E L+RPLCWLEHLSLD+ETGLDP GIRVR V GLVAADYFA  YF WAVLIENLAKIGY
Sbjct: 128 SEILRRPLCWLEHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGY 187

Query: 223 DGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHF 282
           +GKNLHMA+YDWR++F NTEVRD++LSRLKSKIE+MY TNG  KVVVVPHSMG +YFLHF
Sbjct: 188 EGKNLHMASYDWRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHF 247

Query: 283 MKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTP 342
           +KWVE P P GGGGGG GWCAKHIK+++NIG  FLG PKAVS +LSAE + +A  R++ P
Sbjct: 248 LKWVETPLPDGGGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAP 307

Query: 343 GFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNC--DLKKVDT 402
           G L SE+L  QTLEHL+R+S +WDS  SLLPKGGE IWGDLD   E+  NC    +K   
Sbjct: 308 GLLDSELLKLQTLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKSSQ 367

Query: 403 HSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKN-SGSSN 462
            S  N +  N S  K   + KEP  YGRI+SF K A+  PSS+LSTLN +EL    G+SN
Sbjct: 368 LSLSNLHKQNYS-LKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSN 427

Query: 463 DSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAE 522
           DS+ SC + W+EY+EM+RESI KV+ N AYT  TV DLLR +APKMM+R ++HFSHGIA+
Sbjct: 428 DST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIAD 487

Query: 523 DLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK-SIPF 582
           DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK SIPF
Sbjct: 488 DLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPF 547

Query: 583 RIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRH 642
           RID S++G    CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++REY+H
Sbjct: 548 RIDGSLDG-DDVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKH 607

Query: 643 KAPASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINL 695
           K P SLLE RG ES +HVDIMGNV LIED+LR+AAGA+G EIGGD+IYSD++ +SERI++
Sbjct: 608 KPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISI 665

BLAST of Lsi10G012050 vs. TAIR10
Match: AT5G13640.1 (AT5G13640.1 phospholipid:diacylglycerol acyltransferase)

HSP 1 Score: 788.1 bits (2034), Expect = 4.4e-228
Identity = 383/661 (57.94%), Postives = 478/661 (72.31%), Query Frame = 1

Query: 47  QMKQDKDKKKK-----RNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTL 106
           ++  D+D +KK     ++ + K    G+W C+D+CCW IG +C  WW + FLY+ +P + 
Sbjct: 20  EVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASF 79

Query: 107 PGFRAI-------DSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLW 166
           P +          D PG  LK+EG+   HPVV +PGIVTGGLELW+GK CA+GLFRKRLW
Sbjct: 80  PQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLW 139

Query: 167 GGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLA 226
           GG+F E  KRPLCW+EH+SLDNETGLDP GIRVR V GLVAADYFA GYFVWAVLI NLA
Sbjct: 140 GGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLA 199

Query: 227 KIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLY 286
            IGY+ KN++MAAYDWR++FQNTEVRD+ LSR+KS IE+M  TNGG K V+VPHSMGVLY
Sbjct: 200 HIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLY 259

Query: 287 FLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLR 346
           FLHFMKWVEAP P+GGGGG   WCAK+IKA+MNIG  FLG PKAV+ + SAE + VA+ R
Sbjct: 260 FLHFMKWVEAPAPLGGGGGP-DWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVAR 319

Query: 347 AMTPGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKV 406
           A+ PGFL ++I   QTL+H++R++RTWDST S+LPKGG+TIWG LDWSPE    C  KK 
Sbjct: 320 AIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKK- 379

Query: 407 DTHSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSS 466
                +  N++     +    +K PV+YGR+ISF KE A    SE++ ++        S 
Sbjct: 380 -----QKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSI 439

Query: 467 NDSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIA 526
              + +C DVWTEY +M    I+ ++  K YT     DLL  VAPKMM R  +HFS+GIA
Sbjct: 440 --PNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIA 499

Query: 527 EDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRI 586
           +DLDD KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I
Sbjct: 500 DDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQI 559

Query: 587 DLSI-EGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 646
             S  E     CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H 
Sbjct: 560 FTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHS 619

Query: 647 APASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLK 695
            PA+LLEGRG +S +HVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LK
Sbjct: 620 PPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLK 671

BLAST of Lsi10G012050 vs. TAIR10
Match: AT1G04010.1 (AT1G04010.1 phospholipid sterol acyl transferase 1)

HSP 1 Score: 80.9 bits (198), Expect = 3.5e-15
Identity = 57/212 (26.89%), Postives = 87/212 (41.04%), Query Frame = 1

Query: 126 VLVPGIVTGGLELWDGKPCAEGLFRKR----LWGGSFTETLKRPLCWLEHLSLDNETGLD 185
           +++PG  +  L  W    C            +W  + T+ L    CW + + LD     D
Sbjct: 44  IIIPGFASTQLRAWSILDCPYTPLDFNPLDLVWLDT-TKLLSAVNCWFKCMVLDPYNQTD 103

Query: 186 PPGIRVRPVEGLVAADYFAQGYF------VWAVLIENLAKIGYDGKNLHMAAYDWRIAFQ 245
            P  + RP  GL A      GY       VW   ++   + G +   +    YDWR++  
Sbjct: 104 HPECKSRPDSGLSAITELDPGYITGPLSTVWKEWLKWCVEFGIEANAIVAVPYDWRLSPT 163

Query: 246 NTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVE---APPPMGGGG 305
             E RD    +LK   E   +  GG  +V   HSMG   F +F++W+    AP       
Sbjct: 164 KLEERDLYFHKLKLTFETALKLRGGPSIVFA-HSMGNNVFRYFLEWLRLEIAPKHY---- 223

Query: 306 GGLGWCAKHIKAIMNIGSTFLGTPKAVSKILS 325
             L W  +HI A   +G+  LG+ +A+   LS
Sbjct: 224 --LKWLDQHIHAYFAVGAPLLGSVEAIKSTLS 247

BLAST of Lsi10G012050 vs. TAIR10
Match: AT3G03310.1 (AT3G03310.1 lecithin:cholesterol acyltransferase 3)

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-06
Identity = 67/229 (29.26%), Postives = 92/229 (40.17%), Query Frame = 1

Query: 96  YLPLTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWG 155
           ++P    G    ++ G V  R+      PV+LV GI  GG  L   K  ++   R  +W 
Sbjct: 3   WIPCPCWGTNDDENAGEVADRD------PVLLVSGI--GGSILHSKKKNSKSEIR--VWV 62

Query: 156 GSFTETLK-RPLCWLEHLSLDN-ETGLDPP---GIRVRPVE---GLVAADYFAQGYFV-- 215
             F   L  +   W    SL N +TG   P    I V   +   GL A D     +FV  
Sbjct: 63  RIFLANLAFKQSLW----SLYNPKTGYTEPLDDNIEVLVPDDDHGLYAIDILDPSWFVKL 122

Query: 216 --------WAVLIENLAKIGYD-GKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYE 275
                   +  +IE L   GY  G  L    YD+R   Q+  + D  +  LK K+E  Y+
Sbjct: 123 CHLTEVYHFHDMIEMLVGCGYKKGTTLFGYGYDFR---QSNRI-DLLILGLKKKLETAYK 182

Query: 276 TNGGNKVVVVPHSMGVLYFLHFM------------KWVEAPPPMGGGGG 294
            +GG KV ++ HSMG L    FM            KW+    P  G  G
Sbjct: 183 RSGGRKVTIISHSMGGLMVSCFMYLHPEAFSKYVNKWITIATPFQGAPG 213

BLAST of Lsi10G012050 vs. TAIR10
Match: AT4G19860.1 (AT4G19860.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-06
Identity = 53/203 (26.11%), Postives = 81/203 (39.90%), Query Frame = 1

Query: 122 LHPVVLVPGIVTGGLE-----------LWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLE 181
           L+PV+LVPGI    L            +W     A+  FR ++W      T K       
Sbjct: 31  LNPVLLVPGIAGSILNAVDHENGNEERVWVRIFGADHEFRTKMWSRFDPSTGKT------ 90

Query: 182 HLSLDNETGLDPPGIRVRPVEGLVAADYFAQG--------YFVWAVLIENLAKIGYDGKN 241
            +SLD +T +  P  R     GL A D             Y+   +++E +     +GK 
Sbjct: 91  -ISLDPKTSIVVPQDRA----GLHAIDVLDPDMIVGRESVYYFHEMIVEMIGWGFEEGKT 150

Query: 242 LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFM--- 294
           L    YD+R   Q+  +++  L +   K+E +Y+ +G  K+ V+ HSMG L    FM   
Sbjct: 151 LFGFGYDFR---QSNRLQE-TLDQFAKKLETVYKASGEKKINVISHSMGGLLVKCFMGLH 210

BLAST of Lsi10G012050 vs. NCBI nr
Match: gi|659117529|ref|XP_008458650.1| (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo])

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 673/694 (96.97%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKSEDK NVIVAYVKKLMQ KQDKD K+KRNK
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 173

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           N  +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL++PGF AIDSPGAVLKREGIS
Sbjct: 174 NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 233

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 234 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 293

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 294 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 353

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNG NKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGG LGWCAK
Sbjct: 354 DRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGG-LGWCAK 413

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+IMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 414 HIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 473

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 474 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 533

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELSTLN EELKNSG SNDSSFSCSDVWTEYDEM+R+SIRKVS
Sbjct: 534 HYGRIISFSKEAATRPSSELSTLNLEELKNSGGSNDSSFSCSDVWTEYDEMSRDSIRKVS 593

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 594 ENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 653

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           +MEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 654 NMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 713

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           +VSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 714 IVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 773

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 774 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 803

BLAST of Lsi10G012050 vs. NCBI nr
Match: gi|449456178|ref|XP_004145827.1| (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativus])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 672/694 (96.83%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKS++K NVIVAYVK LMQ KQDKD KKKRNK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           NI +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL+LPGF  IDSPGAVLKREGIS
Sbjct: 61  NINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGG LGWCAK
Sbjct: 241 DRALSRLKSKIEVMYATNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGG-LGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELST N EELKNSG SNDSSFSCSDVWTEYDEM+RESIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTQNLEELKNSGGSNDSSFSCSDVWTEYDEMSRESIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTV+TVFDLLR+VAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVETVFDLLRIVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           VVSSGFMCAKGWRNKTRFNPSGSPT+VREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 VVSSGFMCAKGWRNKTRFNPSGSPTHVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of Lsi10G012050 vs. NCBI nr
Match: gi|225436373|ref|XP_002271001.1| (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Vitis vinifera])

HSP 1 Score: 986.9 bits (2550), Expect = 1.8e-284
Identity = 479/697 (68.72%), Postives = 560/697 (80.34%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MAS+LR RKL +V PVK SS+G +  E  K E K +     VK +     +K KKK    
Sbjct: 1   MASILRFRKLCYVEPVKCSSLGFESFETPKIEKKDDR--GAVKAVESTVGEKQKKKS--- 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
              K Q  EW+C+D CC  +GY+CT WWL+ FLYH LP  LPGF+  ++PGA L+REG++
Sbjct: 61  --SKRQPREWRCMDYCCLTVGYVCTAWWLLLFLYHCLPAALPGFQVPEAPGARLRREGLT 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
            LHPVVLVPGIVTGGLELW+G+PC+EGLFRKRLWGGSFTE  KRPLCWLEHL+L N+TGL
Sbjct: 121 ALHPVVLVPGIVTGGLELWEGRPCSEGLFRKRLWGGSFTEIFKRPLCWLEHLTLHNQTGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVR V GLVAADYFA GYFVWAVLIENLA+IGY+GKNL+MAAYDWR++FQNTE+R
Sbjct: 181 DPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLARIGYEGKNLYMAAYDWRLSFQNTEIR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           D+ALSRLKSKIE+M+ TNG  KVVVVPHSMGV+YF HF+KWVE+PPPM GGGGG GWCAK
Sbjct: 241 DQALSRLKSKIELMFVTNGNKKVVVVPHSMGVIYFFHFLKWVESPPPM-GGGGGTGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIG  FLG PKAVS I SAE + VA +R+M PG L SEILG QTLEH++RV RT
Sbjct: 301 HIKAIMNIGPAFLGVPKAVSNIFSAEAKDVAFVRSMDPGVLDSEILGLQTLEHVMRVCRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDL--KKVDTHSFRNENDSNDSKRKKAFQEKE 420
           WDS  SL+PKGGETIWG+LDWSPE+  NCDL  K+    S  + ND+N S  K  FQ KE
Sbjct: 361 WDSIISLIPKGGETIWGNLDWSPEEGYNCDLAKKRYAQPSLLDSNDTN-SDGKMEFQIKE 420

Query: 421 PVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRK 480
            V YGRIISF K  +  PSS+L  L S++   + +    +  CS+VWTEYDEM+R+ IRK
Sbjct: 421 SVRYGRIISFGKATSQLPSSQLPNLGSKDFLRTSNPTKFNSLCSEVWTEYDEMSRDGIRK 480

Query: 481 VSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPD 540
           ++ NKAYT +T  DLLR VAPKMMQR ++ FSHGIA++LDDPKYAHYKYWSNPLETKLPD
Sbjct: 481 IAENKAYTARTFLDLLRFVAPKMMQRAEAQFSHGIADNLDDPKYAHYKYWSNPLETKLPD 540

Query: 541 APDMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDD 600
           APDMEIYCLYGVG+PTERSY+Y  S  D+CKSIPFRID S EG   GCL+ G+YFVDGD+
Sbjct: 541 APDMEIYCLYGVGLPTERSYVYKLSPTDRCKSIPFRIDSSAEGSDGGCLKGGVYFVDGDE 600

Query: 601 SVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVN 660
           SVPV+S+GFMCAKGW+ +TRFNPSG  TYVREYRHK PASL EGRGVES +HVDIMGNV 
Sbjct: 601 SVPVLSAGFMCAKGWKGRTRFNPSGIATYVREYRHKPPASLFEGRGVESGAHVDIMGNVA 660

Query: 661 LIEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           LIED+LRVAAGATG E+GGD+IYSDI+ +S+RINL+L
Sbjct: 661 LIEDVLRVAAGATGVEMGGDRIYSDIMRMSDRINLRL 688

BLAST of Lsi10G012050 vs. NCBI nr
Match: gi|697143286|ref|XP_009625751.1| (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Nicotiana tomentosiformis])

HSP 1 Score: 986.5 bits (2549), Expect = 2.4e-284
Identity = 480/697 (68.87%), Postives = 570/697 (81.78%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQ-DKDKKKKRN 60
           MAS+LR RKL FV PVK SS+     E  K++DK +++    KK   +   +K  +K ++
Sbjct: 1   MASILRFRKLCFVEPVKCSSVS---FEKPKNDDKKSILEPPPKKENLVSAIEKVLEKPKS 60

Query: 61  KNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGI 120
           K IKK Q  EWKC+D+CCW+IGY+CT WWL+ FL ++LP  LPG +  ++PG  LKREG+
Sbjct: 61  KKIKK-QPKEWKCIDSCCWLIGYLCTTWWLLLFLCNFLPANLPGLKVPEAPGVRLKREGL 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
             LHPVVLVPGIVTGGLELW+G+PC+EGLFRKRLWGGSFTE  KRPLCWLEHLSLDNETG
Sbjct: 121 IALHPVVLVPGIVTGGLELWEGRPCSEGLFRKRLWGGSFTEIFKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRVR V GLVAADYFA GYFVWAVLIENLAKIGY+ KN+HMAAYDWR++FQNTE+
Sbjct: 181 LDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEQKNMHMAAYDWRLSFQNTEI 240

Query: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCA 300
           RD++LSRLKSKIE+MY TNG  KVVVVPHSMGV+YFLHF+KWVEAPPP+GGGGG  GWCA
Sbjct: 241 RDQSLSRLKSKIELMYVTNGYKKVVVVPHSMGVIYFLHFLKWVEAPPPVGGGGGS-GWCA 300

Query: 301 KHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSR 360
           KHIKAIMNIG  FLG PKAV+ ILSAE + VA +RAM PG L SE  GFQTL+H++RVSR
Sbjct: 301 KHIKAIMNIGPAFLGVPKAVANILSAEGKDVAFIRAMAPGLLDSETFGFQTLQHVMRVSR 360

Query: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD-THSFRNENDSNDSKRKKAFQEKE 420
           TWDS  SL+P+GGETIWGDL+WSPE    C   K     S   EN+ ND+  +++FQEKE
Sbjct: 361 TWDSVVSLVPRGGETIWGDLNWSPEVENICHKSKARYLQSSSKENNGNDTDVRRSFQEKE 420

Query: 421 PVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRK 480
              YGRI+SF KEA   PSS+LS ++S+E     +S +S+ SC +V TEYDEM+R+SI+K
Sbjct: 421 LPKYGRIVSFGKEALELPSSQLSIIDSKEFVCKSTSRNSNTSCGEVLTEYDEMSRKSIKK 480

Query: 481 VSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPD 540
           V+ N+AYT  T+ DLLR VAPKMM+R ++H SHGIAEDLDDPKY H+KYWSNPLETKLPD
Sbjct: 481 VAENQAYTATTLLDLLRFVAPKMMKRAEAHLSHGIAEDLDDPKYNHHKYWSNPLETKLPD 540

Query: 541 APDMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDD 600
           APDMEIYC YGVGIPTERSY+Y  SS DKCKSIP +ID S +   +GCL+ G++FVDGD+
Sbjct: 541 APDMEIYCSYGVGIPTERSYVYKLSSSDKCKSIPLQIDSSADDSDNGCLKGGVHFVDGDE 600

Query: 601 SVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVN 660
           SVPVVS+GFMCAKGWR+ TRFNPSG  TYVREY+HKAPASLLEGRG ES +HVDIMGNV 
Sbjct: 601 SVPVVSAGFMCAKGWRDNTRFNPSGISTYVREYQHKAPASLLEGRGTESGAHVDIMGNVA 660

Query: 661 LIEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           LIED+LRVAAGATGAE+GGD+IYS+I+ +SERIN++L
Sbjct: 661 LIEDVLRVAAGATGAELGGDRIYSEIMKMSERINIRL 692

BLAST of Lsi10G012050 vs. NCBI nr
Match: gi|1009114106|ref|XP_015873505.1| (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Ziziphus jujuba])

HSP 1 Score: 986.5 bits (2549), Expect = 2.4e-284
Identity = 482/698 (69.05%), Postives = 569/698 (81.52%), Query Frame = 1

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MA VLR RKL +V PV+ SS+G Q  +  ++  K +  V      ++    + K+K++ K
Sbjct: 1   MAWVLRFRKLCYVEPVECSSVGFQSFDQSQNSGKKDDAVG---SKVEESVLETKQKQKTK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
             KK Q+ +W C+D CCW IGY+CT WWL+  L+H    TLPGF+  +SPGA L+REG++
Sbjct: 61  QSKK-QAKDWSCIDYCCWTIGYICTTWWLLLLLFHCFTTTLPGFQVPESPGARLRREGLA 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
            LHPVVLVPGIVTGGLELW+G+ CA+GLFRKRLWGGSFTE  KRPLCWLEHLSLDNETGL
Sbjct: 121 ALHPVVLVPGIVTGGLELWEGRSCADGLFRKRLWGGSFTEIFKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVR V GLVAADYFA GYFVWAVLIENLAKIGY+GKNLHMAAYDWR++FQNTE+R
Sbjct: 181 DPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEGKNLHMAAYDWRLSFQNTEIR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           D ALSRLKSKIE+MY TNG  KVVVVPHSMGV+YFLHFMKWVE+PPP+GGGGG  GWCAK
Sbjct: 241 DHALSRLKSKIELMYVTNGYKKVVVVPHSMGVIYFLHFMKWVESPPPIGGGGGA-GWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKA+MNIG  FLG PKAVS I SAE + +A +RAM PG   SEILG QT EH++RV RT
Sbjct: 301 HIKAVMNIGPAFLGVPKAVSNIFSAEGKDIAYVRAMAPGVFDSEILGLQTFEHVMRVLRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDL--KKVDTHSFRNENDSNDSKRKKAFQEKE 420
           WDST SLLPKGG+TIWG+LDWSPE+  +C+L  KK +  +  + N  N S R KAF  +E
Sbjct: 361 WDSTVSLLPKGGDTIWGNLDWSPEEGHSCNLAKKKNEQPTSCDINSFNHSDRAKAFHVRE 420

Query: 421 PVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSF-SCSDVWTEYDEMNRESIR 480
           P  YGR+ISF KE +  PS +L TLN EEL   G+S  +SF SC +V TEYD+++RESIR
Sbjct: 421 PEKYGRMISFGKETSQLPSPQLPTLNLEELLGMGTS--ASFNSCGEVLTEYDKLSRESIR 480

Query: 481 KVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLP 540
           K++ NKAYT KT+FDLLR VAPK MQR ++HFSHGIA++LDDPKYA+YKYWSNPLETKLP
Sbjct: 481 KIAENKAYTAKTLFDLLRFVAPKTMQRAEAHFSHGIADNLDDPKYANYKYWSNPLETKLP 540

Query: 541 DAPDMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGD 600
           +APDMEIYCLYGVG+PTERSY+Y  SS DKCKSIPFRID S +G+   CL++G+YFVDGD
Sbjct: 541 NAPDMEIYCLYGVGMPTERSYVYKLSSSDKCKSIPFRIDSSADGEAGNCLKNGVYFVDGD 600

Query: 601 DSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNV 660
           +SVPV+S+GFMCAKGWR +TRFNPSG  TY+REYRHK PASLLEGRG+ES +HVDIMGNV
Sbjct: 601 ESVPVISAGFMCAKGWRGRTRFNPSGIATYIREYRHKPPASLLEGRGLESGAHVDIMGNV 660

Query: 661 NLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
            LIED+L VAAGATGAEIG DKIYSDI+ +SE+INL+L
Sbjct: 661 ALIEDVLLVAAGATGAEIGSDKIYSDIMRMSEKINLRL 691

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PDAT2_ARATH2.3e-25565.41Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana G... [more]
PDAT1_ARATH7.8e-22757.94Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana GN=PDAT1 P... [more]
PDAT_SCHPO3.7e-5939.93Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain... [more]
PDAT_YEAST5.9e-5738.76Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ... [more]
LCAT2_ARATH6.2e-1426.89Phospholipid--sterol O-acyltransferase OS=Arabidopsis thaliana GN=PSAT PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KGM0_CUCSA0.0e+0094.67Uncharacterized protein OS=Cucumis sativus GN=Csa_6G149960 PE=4 SV=1[more]
F6GW64_VITVI1.3e-28468.72Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0061g01110 PE=4 SV=... [more]
M5W8A4_PRUPE2.0e-28268.10Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015987mg PE=4 SV=1[more]
A0A067DI02_CITSI2.7e-28267.91Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005610mg PE=4 SV=1[more]
V4SFM5_9ROSI2.7e-28267.91Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027727mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G44830.11.3e-25665.41 Lecithin:cholesterol acyltransferase family protein[more]
AT5G13640.14.4e-22857.94 phospholipid:diacylglycerol acyltransferase[more]
AT1G04010.13.5e-1526.89 phospholipid sterol acyl transferase 1[more]
AT3G03310.11.7e-0629.26 lecithin:cholesterol acyltransferase 3[more]
AT4G19860.11.7e-0626.11 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659117529|ref|XP_008458650.1|0.0e+0094.67PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo][more]
gi|449456178|ref|XP_004145827.1|0.0e+0094.67PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativ... [more]
gi|225436373|ref|XP_002271001.1|1.8e-28468.72PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Vitis vinifer... [more]
gi|697143286|ref|XP_009625751.1|2.4e-28468.87PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Nicotiana tom... [more]
gi|1009114106|ref|XP_015873505.1|2.4e-28469.05PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Ziziphus juju... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008374O-acyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR003386LACT/PDAT_acylTrfase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0009873 ethylene-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
cellular_component GO:0005622 intracellular
molecular_function GO:0016298 lipase activity
molecular_function GO:0008374 O-acyltransferase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G012050.1Lsi10G012050.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003386Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferasePFAMPF02450LCATcoord: 149..654
score: 2.3
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 47..694
score:
NoneNo IPR availablePANTHERPTHR11440:SF54PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATEDcoord: 47..694
score:

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi10G012050Silver-seed gourdcarlsiB838
Lsi10G012050Cucumber (Chinese Long) v3cuclsiB005
Lsi10G012050Cucumber (Chinese Long) v3cuclsiB444
Lsi10G012050Watermelon (97103) v2lsiwmbB033
Lsi10G012050Watermelon (97103) v2lsiwmbB040
Lsi10G012050Watermelon (97103) v2lsiwmbB067
Lsi10G012050Wax gourdlsiwgoB087
Lsi10G012050Bottle gourd (USVL1VR-Ls)lsilsiB040
Lsi10G012050Bottle gourd (USVL1VR-Ls)lsilsiB025
Lsi10G012050Bottle gourd (USVL1VR-Ls)lsilsiB042
Lsi10G012050Cucurbita maxima (Rimu)cmalsiB227
Lsi10G012050Cucurbita maxima (Rimu)cmalsiB540
Lsi10G012050Cucurbita maxima (Rimu)cmalsiB744
Lsi10G012050Cucurbita maxima (Rimu)cmalsiB780
Lsi10G012050Cucurbita moschata (Rifu)cmolsiB215
Lsi10G012050Cucurbita moschata (Rifu)cmolsiB525
Lsi10G012050Cucurbita moschata (Rifu)cmolsiB736
Lsi10G012050Cucurbita moschata (Rifu)cmolsiB772
Lsi10G012050Cucurbita pepo (Zucchini)cpelsiB389
Lsi10G012050Cucurbita pepo (Zucchini)cpelsiB487
Lsi10G012050Cucurbita pepo (Zucchini)cpelsiB591
Lsi10G012050Wild cucumber (PI 183967)cpilsiB006
Lsi10G012050Wild cucumber (PI 183967)cpilsiB423
Lsi10G012050Cucumber (Chinese Long) v2culsiB005
Lsi10G012050Cucumber (Chinese Long) v2culsiB421
Lsi10G012050Melon (DHL92) v3.5.1lsimeB070
Lsi10G012050Melon (DHL92) v3.5.1lsimeB076
Lsi10G012050Watermelon (Charleston Gray)lsiwcgB037
Lsi10G012050Watermelon (Charleston Gray)lsiwcgB068
Lsi10G012050Watermelon (97103) v1lsiwmB048
Lsi10G012050Watermelon (97103) v1lsiwmB064
Lsi10G012050Cucumber (Gy14) v2cgyblsiB005
Lsi10G012050Cucumber (Gy14) v2cgyblsiB146
Lsi10G012050Cucumber (Gy14) v2cgyblsiB384
Lsi10G012050Melon (DHL92) v3.6.1lsimedB072
Lsi10G012050Melon (DHL92) v3.6.1lsimedB078
Lsi10G012050Silver-seed gourdcarlsiB222