Carg03187 (gene) Silver-seed gourd

Overview
NameCarg03187
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPhospholipid:diacylglycerol acyltransferase
LocationCucurbita_argyrosperma_scaffold_005 : 532306 .. 533177 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTACGTCAACCCTGTTAAGAATTCTTCTATGGGGATCCGAACCATTGATGATGCACATAAATCAGAGGATAAACCTAATGTTGTTGTTGCATATGTGAAGAAAATGCTGCAAAAGAAGCAAGATAAGGACAGGAAGAAGAAAAGGAGCAAGAACATCAATAAGGGGCAATCAAGAGAATGGAAGTGCTTAGATAACTGCTGTTGGATTATTGGATATATGTGTACATTTTGGTGGCTGATGTTTTTCGTCTACCATTATTTGCCTCTTACATTACCTGGCTTTCCAGCGATTGACTCTCCGGGGGCTGTTTTAAAGCGAGATGGCGTATCAGGTCTTCACCCAGTCGTTTTAGTGCCCGGCATTGTCACCGGCGGGCTGGAGCTCTGGGATGGCAAGCCCTGCGCCAAGGGACTGTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGGTTAGCGAATTCCAGATGAATTTTACATTCTGAAGCTTTTGGATTGATACTTGAAGTACCCATTTGAGAATTTGAATTCAAACTTCCAGACCATTATGCTGGTTGGAGCACCTGTCTCTGGATAATGAAACAGGGCTTGACCCACCAGGCATTCGAATCCGGCCAGTCGAAGGCCTTGTGGCCGCTGATTATTTCACCCAAGGCTACTTCGTTTGGGCTGTTCTCATAGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTACATATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCAACGTGACCAAAAACACTCTCTTCTTCTTCTTCCAATCAAATTATACATTTGATCATTGA

mRNA sequence

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTACGTCAACCCTGTTAAGAATTCTTCTATGGGGATCCGAACCATTGATGATGCACATAAATCAGAGGATAAACCTAATGTTGTTGTTGCATATGTGAAGAAAATGCTGCAAAAGAAGCAAGATAAGGACAGGAAGAAGAAAAGGAGCAAGAACATCAATAAGGGGCAATCAAGAGAATGGAAGTGCTTAGATAACTGCTGTTGGATTATTGGATATATGTGTACATTTTGGTGGCTGATGTTTTTCGTCTACCATTATTTGCCTCTTACATTACCTGGCTTTCCAGCGATTGACTCTCCGGGGGCTGTTTTAAAGCGAGATGGCGTATCAGGTCTTCACCCAGTCGTTTTAGTGCCCGGCATTGTCACCGGCGGGCTGGAGCTCTGGGATGGCAAGCCCTGCGCCAAGGGACTGTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCATTATGCTGGTTGGAGCACCTGTCTCTGGATAATGAAACAGGGCTTGACCCACCAGGCATTCGAATCCGGCCAGTCGAAGGCCTTGTGGCCGCTGATTATTTCACCCAAGGCTACTTCGTTTGGGCTGTTCTCATAGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTACATATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCAACGTGACCAAAAACACTCTCTTCTTCTTCTTCCAATCAAATTATACATTTGATCATTGA

Coding sequence (CDS)

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTACGTCAACCCTGTTAAGAATTCTTCTATGGGGATCCGAACCATTGATGATGCACATAAATCAGAGGATAAACCTAATGTTGTTGTTGCATATGTGAAGAAAATGCTGCAAAAGAAGCAAGATAAGGACAGGAAGAAGAAAAGGAGCAAGAACATCAATAAGGGGCAATCAAGAGAATGGAAGTGCTTAGATAACTGCTGTTGGATTATTGGATATATGTGTACATTTTGGTGGCTGATGTTTTTCGTCTACCATTATTTGCCTCTTACATTACCTGGCTTTCCAGCGATTGACTCTCCGGGGGCTGTTTTAAAGCGAGATGGCGTATCAGGTCTTCACCCAGTCGTTTTAGTGCCCGGCATTGTCACCGGCGGGCTGGAGCTCTGGGATGGCAAGCCCTGCGCCAAGGGACTGTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCATTATGCTGGTTGGAGCACCTGTCTCTGGATAATGAAACAGGGCTTGACCCACCAGGCATTCGAATCCGGCCAGTCGAAGGCCTTGTGGCCGCTGATTATTTCACCCAAGGCTACTTCGTTTGGGCTGTTCTCATAGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTACATATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCAACGTGACCAAAAACACTCTCTTCTTCTTCTTCCAATCAAATTATACATTTGATCATTGA

Protein sequence

MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRSKNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVCNVTKNTLFFFFQSNYTFDH
Homology
[Close]
BLAST of Carg03187 vs. NCBI nr
Match: XP_023512597.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.5 bits (1251), Expect = 5.7e-134
Identity = 232/240 (96.67%), Postives = 235/240 (97.92%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLYYVNPVKNSSMGI++IDDAHKSEDKPNVVVAYVKKMLQKKQD   XXXX+
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQSIDDAHKSEDKPNVVVAYVKKMLQKKQDNXXXXXXN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPG VLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGTVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFT GYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTHGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

[Close]
BLAST of Carg03187 vs. NCBI nr
Match: XP_022944139.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 473.4 bits (1217), Expect = 5.0e-130
Identity = 228/240 (95.00%), Postives = 229/240 (95.42%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLYYVNPVKNSSMGI+TIDDAHKSEDKPNVVVAYVKKMLQKK     XXXX 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKXXXXXXXXXX 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
                GQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  XXXXXGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

[Close]
BLAST of Carg03187 vs. NCBI nr
Match: XP_022986276.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 473.4 bits (1217), Expect = 5.0e-130
Identity = 228/240 (95.00%), Postives = 229/240 (95.42%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLYYVNPVKNSSMGI+TIDDAHKSEDKPNVVVAYVKKMLQKK     XXXX 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKXXXXXXXXXX 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
                GQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  XXXXXGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

[Close]
BLAST of Carg03187 vs. NCBI nr
Match: XP_004145827.1 (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativus] >KGN46906.1 hypothetical protein Csa_6G149960 [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 1.1e-124
Identity = 209/240 (87.08%), Postives = 229/240 (95.42%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKS++K NV+VAYVK ++QKKQDKD     +
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNIN+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL+LPGFP IDSPGAVLKR+G+
Sbjct: 61  KNINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGI 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 239

[Close]
BLAST of Carg03187 vs. NCBI nr
Match: XP_008458650.1 (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo])

HSP 1 Score: 451.4 bits (1160), Expect = 2.0e-123
Identity = 212/240 (88.33%), Postives = 230/240 (95.83%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKSEDK NV+VAYVKK++QKKQD   XXXX 
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSEDKRNVIVAYVKKLMQKKQDXXXXXXXX 173

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
            N N+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL++PGFPAIDSPGAVLKR+G+
Sbjct: 174 XNTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGI 233

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 234 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 293

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 294 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 352

[Close]
BLAST of Carg03187 vs. TAIR10
Match: AT3G44830.1 (Lecithin:cholesterol acyltransferase family protein)

HSP 1 Score: 279.3 bits (713), Expect = 2.5e-75
Identity = 122/174 (70.11%), Postives = 144/174 (82.76%), Query Frame = 0 

Query: 67  QSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGVSGLHPV 126
           +S    C+D+CCW+IGY+CT WWL+ F+YH +P+      A +SPG  L RDGV   HPV
Sbjct: 35  RSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVPVPAM-LQAPESPGTRLSRDGVKAFHPV 94

Query: 127 VLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGI 186
           +LVPGIVTGGLELW+G+PCA+GLFRKRLWG SF+E L+RPLCWLEHLSLD+ETGLDP GI
Sbjct: 95  ILVPGIVTGGLELWEGRPCAEGLFRKRLWGASFSEILRRPLCWLEHLSLDSETGLDPSGI 154

Query: 187 RIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 241
           R+R V GLVAADYF   YF WAVLIENLAKIGY+GKNLHMA+YDWR++F NTEV
Sbjct: 155 RVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYDWRLSFHNTEV 207

[Close]
BLAST of Carg03187 vs. TAIR10
Match: AT5G13640.1 (phospholipid:diacylglycerol acyltransferase)

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-70
Identity = 114/179 (63.69%), Postives = 138/179 (77.09%), Query Frame = 0 

Query: 73  CLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGF-------PAIDSPGAVLKRDGVSGLHP 132
           C+D+CCW IG +C  WW + F+Y+ +P + P +       P  D PG  LK++G+   HP
Sbjct: 50  CIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHP 109

Query: 133 VVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPG 192
           VV +PGIVTGGLELW+GK CA GLFRKRLWGG+F E  KRPLCW+EH+SLDNETGLDP G
Sbjct: 110 VVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAG 169

Query: 193 IRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVCNVT 245
           IR+R V GLVAADYF  GYFVWAVLI NLA IGY+ KN++MAAYDWR++FQNTEV + T
Sbjct: 170 IRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQT 228

[Close]
BLAST of Carg03187 vs. Swiss-Prot
Match: sp|Q9FYC7|PDAT2_ARATH (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 4.5e-74
Identity = 122/174 (70.11%), Postives = 144/174 (82.76%), Query Frame = 0 

Query: 67  QSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGVSGLHPV 126
           +S    C+D+CCW+IGY+CT WWL+ F+YH +P+      A +SPG  L RDGV   HPV
Sbjct: 35  RSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVPVPAM-LQAPESPGTRLSRDGVKAFHPV 94

Query: 127 VLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGI 186
           +LVPGIVTGGLELW+G+PCA+GLFRKRLWG SF+E L+RPLCWLEHLSLD+ETGLDP GI
Sbjct: 95  ILVPGIVTGGLELWEGRPCAEGLFRKRLWGASFSEILRRPLCWLEHLSLDSETGLDPSGI 154

Query: 187 RIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 241
           R+R V GLVAADYF   YF WAVLIENLAKIGY+GKNLHMA+YDWR++F NTEV
Sbjct: 155 RVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYDWRLSFHNTEV 207

[Close]
BLAST of Carg03187 vs. Swiss-Prot
Match: sp|Q9FNA9|PDAT1_ARATH (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 3.3e-69
Identity = 114/179 (63.69%), Postives = 138/179 (77.09%), Query Frame = 0 

Query: 73  CLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGF-------PAIDSPGAVLKRDGVSGLHP 132
           C+D+CCW IG +C  WW + F+Y+ +P + P +       P  D PG  LK++G+   HP
Sbjct: 50  CIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHP 109

Query: 133 VVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPG 192
           VV +PGIVTGGLELW+GK CA GLFRKRLWGG+F E  KRPLCW+EH+SLDNETGLDP G
Sbjct: 110 VVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAG 169

Query: 193 IRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVCNVT 245
           IR+R V GLVAADYF  GYFVWAVLI NLA IGY+ KN++MAAYDWR++FQNTEV + T
Sbjct: 170 IRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQT 228

[Close]
BLAST of Carg03187 vs. Swiss-Prot
Match: sp|O94680|PDAT_SCHPO (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=plh1 PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 2.5e-32
Identity = 60/130 (46.15%), Postives = 83/130 (63.85%), Query Frame = 0 

Query: 112 GAVLKRDGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWG--GSFTETLKRPLCW 171
           G  +  +G    HPV++VPG+++ GLE W    C+   FRKRLWG             CW
Sbjct: 134 GLDMYNEGYRSDHPVIMVPGVISSGLESWSFNNCSIPYFRKRLWGSWSMLKAMFLDKQCW 193

Query: 172 LEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAY 231
           LEHL LD +TGLDP GI++R  +G  AAD+F  GY++W+ +IENLA IGY+  N+  A+Y
Sbjct: 194 LEHLMLDKKTGLDPKGIKLRAAQGFEAADFFITGYWIWSKVIENLAAIGYEPNNMLSASY 253

Query: 232 DWRIAFQNTE 240
           DWR+++ N E
Sbjct: 254 DWRLSYANLE 263

[Close]
BLAST of Carg03187 vs. Swiss-Prot
Match: sp|P40345|PDAT_YEAST (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LRO1 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.2e-27
Identity = 56/135 (41.48%), Postives = 81/135 (60.00%), Query Frame = 0 

Query: 112 GAVLKRD-GVSGLHPVVLVPGIVTGGLELW----DGKPCAKGLFRKRLWGGSF--TETLK 171
           G  L RD  +   HPVV+VPG+++ G+E W    D +  +   FRKRLWG  +     + 
Sbjct: 160 GKQLLRDYNIEAKHPVVMVPGVISTGIESWGVIGDDECDSSAHFRKRLWGSFYMLRTMVM 219

Query: 172 RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNL 231
             +CWL+H+ LD ETGLDPP   +R  +G  + DYF  GY++W  + +NL  IGY+   +
Sbjct: 220 DKVCWLKHVMLDPETGLDPPNFTLRAAQGFESTDYFIAGYWIWNKVFQNLGVIGYEPNKM 279

Query: 232 HMAAYDWRIAFQNTE 240
             AAYDWR+A+ + E
Sbjct: 280 TSAAYDWRLAYLDLE 294

[Close]
BLAST of Carg03187 vs. TrEMBL
Match: tr|A0A0A0KGM0|A0A0A0KGM0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 7.1e-125
Identity = 209/240 (87.08%), Postives = 229/240 (95.42%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKS++K NV+VAYVK ++QKKQDKD     +
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNIN+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL+LPGFP IDSPGAVLKR+G+
Sbjct: 61  KNINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGI 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 239

[Close]
BLAST of Carg03187 vs. TrEMBL
Match: tr|A0A1S3C9K8|A0A1S3C9K8_CUCME (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497986 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.3e-123
Identity = 212/240 (88.33%), Postives = 230/240 (95.83%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKSEDK NV+VAYVKK++QKKQD   XXXX 
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSEDKRNVIVAYVKKLMQKKQDXXXXXXXX 173

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
            N N+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL++PGFPAIDSPGAVLKR+G+
Sbjct: 174 XNTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGI 233

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 234 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 293

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 294 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 352

[Close]
BLAST of Carg03187 vs. TrEMBL
Match: tr|A0A2P5BX89|A0A2P5BX89_PARAD (Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_202860 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 4.3e-90
Identity = 162/243 (66.67%), Postives = 192/243 (79.01%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTID-DAHKSE--DKPNVVVAYVKKMLQKKQDKDRXX 60
           MASVLR RKL +V PVK SS G +  +  A K+E  D+ +VV +    +L+KK+      
Sbjct: 1   MASVLRFRKLCFVEPVKCSSAGFQCSEKTAQKNEKRDENDVVSSAGYSVLEKKRK----- 60

Query: 61  XXSKNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKR 120
                 +K Q +EW+C+D+CCW IGYMCT WWL+ F+YH LP TLPGF   +SPGA L+R
Sbjct: 61  ------SKKQPKEWRCIDSCCWCIGYMCTTWWLLLFLYHCLPATLPGFQVPESPGARLRR 120

Query: 121 DGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDN 180
           +GV  LHPVVLVPGIVTGGLELW+G+PCA+GLFRKRLWGGSFTE  KRPLCWLEHLSL N
Sbjct: 121 EGVKALHPVVLVPGIVTGGLELWEGRPCAEGLFRKRLWGGSFTEIFKRPLCWLEHLSLHN 180

Query: 181 ETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQN 240
           ETGLDPPGIR+R V GLVAADYF  GYFVWAVLIENLAKIGY+GKN++MAAYDWR++FQN
Sbjct: 181 ETGLDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEGKNMYMAAYDWRLSFQN 232

[Close]
BLAST of Carg03187 vs. TrEMBL
Match: tr|A0A2P5ASR1|A0A2P5ASR1_9ROSA (Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase OS=Trema orientalis OX=63057 GN=TorRG33x02_342340 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 7.4e-90
Identity = 160/242 (66.12%), Postives = 189/242 (78.10%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNV--VVAYVKKMLQKKQDKDRXXX 60
           MASVLR RKL +V PVK SS+G +  +      +K N   VV+ V   + +K+ K     
Sbjct: 1   MASVLRFRKLCFVEPVKCSSVGFQCSEKTTPKNEKKNENDVVSNVGISVLEKKRK----- 60

Query: 61  XSKNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRD 120
                +K Q +EW+C+D+CCW IGYMCT WWL+ F+YH LP TLPGF   +SPGA L+R+
Sbjct: 61  -----SKKQPKEWRCIDSCCWFIGYMCTTWWLLLFLYHCLPATLPGFQVPESPGARLRRE 120

Query: 121 GVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNE 180
           GV  LHPVVLVPGIVTGGLELW+G+PCA+GLFRKRLWGGSFTE  KRPLCWLEHLSL NE
Sbjct: 121 GVKALHPVVLVPGIVTGGLELWEGRPCAEGLFRKRLWGGSFTEIFKRPLCWLEHLSLHNE 180

Query: 181 TGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNT 240
           TGLDPPGIR+R V GLVAADYF  GYFVWAVLIENLAKIGY+GKN++MAAYDWR++FQNT
Sbjct: 181 TGLDPPGIRVRAVPGLVAADYFAPGYFVWAVLIENLAKIGYEGKNMYMAAYDWRLSFQNT 232

[Close]
BLAST of Carg03187 vs. TrEMBL
Match: tr|A0A1Q3BVB1|A0A1Q3BVB1_CEPFO (LACT domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_15203 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.8e-89
Identity = 155/240 (64.58%), Postives = 188/240 (78.33%), Query Frame = 0 

Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRXXXXS 60
           MAS+LR RKL Y+ P+K S        +  K++ K   V++  K  L+  + K+      
Sbjct: 1   MASILRFRKLCYIEPLKFSQ-----YFEHSKNDKKEENVISNDKNALEINKKKN------ 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
               K Q +EW+C+D+CCW+IGYMCT WWL+ F+Y+ LP+TLPGF   ++PG  LKRDG+
Sbjct: 61  -YTTKKQPKEWRCIDSCCWVIGYMCTTWWLLLFLYYCLPVTLPGFQVPEAPGVRLKRDGL 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           + LHPVVLVPGIVTGGLELW+G+PCAKGLFRKRLWGGSFTE  +RPLCWLEH+SL NETG
Sbjct: 121 TALHPVVLVPGIVTGGLELWEGRPCAKGLFRKRLWGGSFTEIFRRPLCWLEHISLHNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPV GLVAADYF  GYFVWAV+IENLAKIGY+GKNLHMAAYDWRI+FQNTE+
Sbjct: 181 LDPPGIRVRPVPGLVAADYFAPGYFVWAVIIENLAKIGYEGKNLHMAAYDWRISFQNTEI 228

The following BLAST results are available for this feature:
BLAST of Carg03187 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023512597.15.7e-13496.67putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pe... [more]
XP_022944139.15.0e-13095.00putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata][more]
XP_022986276.15.0e-13095.00putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima][more]
XP_004145827.11.1e-12487.08PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativ... [more]
XP_008458650.12.0e-12388.33PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo][more]
BLAST of Carg03187 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G44830.12.5e-7570.11Lecithin:cholesterol acyltransferase family protein[more]
AT5G13640.11.8e-7063.69phospholipid:diacylglycerol acyltransferase[more]
BLAST of Carg03187 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9FYC7|PDAT2_ARATH4.5e-7470.11Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana O... [more]
sp|Q9FNA9|PDAT1_ARATH3.3e-6963.69Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
sp|O94680|PDAT_SCHPO2.5e-3246.15Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain... [more]
sp|P40345|PDAT_YEAST3.2e-2741.48Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ... [more]
BLAST of Carg03187 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KGM0|A0A0A0KGM0_CUCSA7.1e-12587.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1[more]
tr|A0A1S3C9K8|A0A1S3C9K8_CUCME1.3e-12388.33putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 G... [more]
tr|A0A2P5BX89|A0A2P5BX89_PARAD4.3e-9066.67Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase OS=Parasponia a... [more]
tr|A0A2P5ASR1|A0A2P5ASR1_9ROSA7.4e-9066.12Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase OS=Trema orient... [more]
tr|A0A1Q3BVB1|A0A1Q3BVB1_CEPFO2.8e-8964.58LACT domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_152... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008374O-acyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR003386LACT/PDAT_acylTrfase
IPR029058AB_hydrolase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008374 O-acyltransferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03187-RACarg03187-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 184..251
e-value: 6.0E-8
score: 34.2
IPR003386Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferasePFAMPF02450LCATcoord: 150..237
e-value: 6.5E-18
score: 65.0
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 1..242
NoneNo IPR availablePANTHERPTHR11440:SF54PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATEDcoord: 1..242

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg03187Cla008683Watermelon (97103) v1carwmB0330
Carg03187Csa6G149960Cucumber (Chinese Long) v2carcuB0371
Carg03187MELO3C021466Melon (DHL92) v3.5.1carmeB0340
Carg03187ClCG02G021780Watermelon (Charleston Gray)carwcgB0327
Carg03187CSPI06G12130Wild cucumber (PI 183967)carcpiB0374
Carg03187Cucsa.385510Cucumber (Gy14) v1carcgyB0403
Carg03187CmaCh20G005410Cucurbita maxima (Rimu)carcmaB0473
Carg03187CmoCh20G005710Cucurbita moschata (Rifu)carcmoB0464
Carg03187Lsi10G012050Bottle gourd (USVL1VR-Ls)carlsiB303
Carg03187Cp4.1LG16g04870Cucurbita pepo (Zucchini)carcpeB0465
Carg03187CsGy6G012190Cucumber (Gy14) v2carcgybB0351
Carg03187CsaV3_6G014580Cucumber (Chinese Long) v3carcucB0375
Carg03187Cla97C02G047850Watermelon (97103) v2carwmbB0346
Carg03187Bhi10G000078Wax gourdcarwgoB0421
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg03187Silver-seed gourdcarcarB187
Carg03187Silver-seed gourdcarcarB287
Carg03187Silver-seed gourdcarcarB366
Carg03187Cucurbita maxima (Rimu)carcmaB0474
Carg03187Cucurbita maxima (Rimu)carcmaB0482
Carg03187Cucurbita moschata (Rifu)carcmoB0460
Carg03187Cucurbita moschata (Rifu)carcmoB0465
Carg03187Cucurbita moschata (Rifu)carcmoB0469
Carg03187Cucurbita pepo (Zucchini)carcpeB0478
Carg03187Melon (DHL92) v3.6.1carmedB0346
Carg03187Watermelon (97103) v2carwmbB0340
Carg03187Watermelon (97103) v2carwmbB0353
Carg03187Wax gourdcarwgoB0423