Lsi10G001710 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi10G001710
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCytochrome P450
Locationchr10 : 2778155 .. 2779057 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTCGCTACGCTTTCCTTCTTCTCCTTCTTGCTTTCCTTCTTCTGCAACAACTCAGAACCCGCTGGAGAAATCTGCCGCCGAGCCCACCCTCTGTTCCGATTATCGGTCATCTCCACCTCTTACAACGCCCAACCCACCGGAACTTCCATAACATCGCCGCCAAATACGGACCCATTTTCACTCTTCGATTCGGATCTCGCCTTGCTCTCATAGTCTCTTCACTCCAAATAGCCCAAGAATGCTTCACCAAAAACGACGTTGTTTTTGCCAACCGTCCACGTTTGCTCATCAGCCAATACCTCGGCTACAACTGCACTACCATGTCAACTGCCCCTTACGGCGACCACTGGCGGAACCTCCGTCGCCTCGCCACCGTGGAGATCTTCTCCACGGCGCGCCTCAACGCGTCTCTCTGCATTCGGAAGGATGAAATCCGGCGATTGTTGGTCAAACTCCATTCGGAATCATTAGCTAAGGTGGAGTTGAAATCGATGTTTTCGGAACTGAGTTTCAACATCGTGATGAGGGTCGTCGCCGGAAAACGGTACTACGGCGAGGAAGTTTCCGATGAGGCGGAGGCGAGGGAGTTCGAGCTTATGGAGGAGATTTCTCGTCATGGAGGAGCTTCGCAATGGATCGATTTCATGCCGATTTTGAAATGGATTGGGTTTGACGGATATGAGAAGAGTTTGGCCAAATTGACGAAAAGGGCCGATCGATTTATGCAGGAATTGGTTGAAGAACGACGGAATAAAAAAGTATTTGCAAGAGAAGAAGAGAGTTCTCTGCTTCAGCGGCTTCTTGAATTGCAGCTCTCTGAACCCGAATATTACACCGATCAGATTATCAAAGGGATCGTTCTGGTTAGTAATCCAACCTCCACTTGTTCAATCTAA

mRNA sequence

ATGGATTCTCGCTACGCTTTCCTTCTTCTCCTTCTTGCTTTCCTTCTTCTGCAACAACTCAGAACCCGCTGGAGAAATCTGCCGCCGAGCCCACCCTCTGTTCCGATTATCGGTCATCTCCACCTCTTACAACGCCCAACCCACCGGAACTTCCATAACATCGCCGCCAAATACGGACCCATTTTCACTCTTCGATTCGGATCTCGCCTTGCTCTCATAGTCTCTTCACTCCAAATAGCCCAAGAATGCTTCACCAAAAACGACGTTGTTTTTGCCAACCGTCCACGTTTGCTCATCAGCCAATACCTCGGCTACAACTGCACTACCATGTCAACTGCCCCTTACGGCGACCACTGGCGGAACCTCCGTCGCCTCGCCACCGTGGAGATCTTCTCCACGGCGCGCCTCAACGCGTCTCTCTGCATTCGGAAGGATGAAATCCGGCGATTGTTGGTCAAACTCCATTCGGAATCATTAGCTAAGGTGGAGTTGAAATCGATGTTTTCGGAACTGAGTTTCAACATCGTGATGAGGGTCGTCGCCGGAAAACGGTACTACGGCGAGGAAGTTTCCGATGAGGCGGAGGCGAGGGAGTTCGAGCTTATGGAGGAGATTTCTCGTCATGGAGGAGCTTCGCAATGGATCGATTTCATGCCGATTTTGAAATGGATTGGGTTTGACGGATATGAGAAGAGTTTGGCCAAATTGACGAAAAGGGCCGATCGATTTATGCAGGAATTGGTTGAAGAACGACGGAATAAAAAAGTATTTGCAAGAGAAGAAGAGAGTTCTCTGCTTCAGCGGCTTCTTGAATTGCAGCTCTCTGAACCCGAATATTACACCGATCAGATTATCAAAGGGATCGTTCTGGTTAGTAATCCAACCTCCACTTGTTCAATCTAA

Coding sequence (CDS)

ATGGATTCTCGCTACGCTTTCCTTCTTCTCCTTCTTGCTTTCCTTCTTCTGCAACAACTCAGAACCCGCTGGAGAAATCTGCCGCCGAGCCCACCCTCTGTTCCGATTATCGGTCATCTCCACCTCTTACAACGCCCAACCCACCGGAACTTCCATAACATCGCCGCCAAATACGGACCCATTTTCACTCTTCGATTCGGATCTCGCCTTGCTCTCATAGTCTCTTCACTCCAAATAGCCCAAGAATGCTTCACCAAAAACGACGTTGTTTTTGCCAACCGTCCACGTTTGCTCATCAGCCAATACCTCGGCTACAACTGCACTACCATGTCAACTGCCCCTTACGGCGACCACTGGCGGAACCTCCGTCGCCTCGCCACCGTGGAGATCTTCTCCACGGCGCGCCTCAACGCGTCTCTCTGCATTCGGAAGGATGAAATCCGGCGATTGTTGGTCAAACTCCATTCGGAATCATTAGCTAAGGTGGAGTTGAAATCGATGTTTTCGGAACTGAGTTTCAACATCGTGATGAGGGTCGTCGCCGGAAAACGGTACTACGGCGAGGAAGTTTCCGATGAGGCGGAGGCGAGGGAGTTCGAGCTTATGGAGGAGATTTCTCGTCATGGAGGAGCTTCGCAATGGATCGATTTCATGCCGATTTTGAAATGGATTGGGTTTGACGGATATGAGAAGAGTTTGGCCAAATTGACGAAAAGGGCCGATCGATTTATGCAGGAATTGGTTGAAGAACGACGGAATAAAAAAGTATTTGCAAGAGAAGAAGAGAGTTCTCTGCTTCAGCGGCTTCTTGAATTGCAGCTCTCTGAACCCGAATATTACACCGATCAGATTATCAAAGGGATCGTTCTGGTTAGTAATCCAACCTCCACTTGTTCAATCTAA

Protein sequence

MDSRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSESLAKVELKSMFSELSFNIVMRVVAGKRYYGEEVSDEAEAREFELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLVSNPTSTCSI
Homology
[Close]
BLAST of Lsi10G001710 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.3e-77
Identity = 152/302 (50.33%), Postives = 212/302 (70.20%), Query Frame = 1 

Query: 1   MDSRYAFLLLLLAFLLLQ-----QLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIA 60
           M + Y  L++ L FL++          +++NLPP P  +PIIG+LH L++P H  FH ++
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLS 60

Query: 61  AKYGPIFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPY 120
            KYG IF+L FGSRL ++VSSL IAQECFTKND+V ANRP  L  +Y+GYN TT++ +PY
Sbjct: 61  QKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPY 120

Query: 121 GDHWRNLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELS 180
           GDHWRNLRR+ ++EI S+ RLN+ L IR+DEI RL+ KL  +S     +VEL+ MFSE++
Sbjct: 121 GDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMT 180

Query: 181 FNIVMRVVAGKRYYGE--EVSDEAEAREFE-LMEEISRHGGASQWIDFMPILKWIGFDGY 240
           FN +MR+V+GKRYYG   +VSD  EAR F  +++E+   GGA+   DF+  L+W  FDG 
Sbjct: 181 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 240

Query: 241 EKSLAKLTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIV 292
           EK L K++KR D F+Q L++E R    F +   ++++  LL  Q S+PEYYTDQIIKG++
Sbjct: 241 EKRLKKISKRTDAFLQGLIDEHR----FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLM 298

[Close]
BLAST of Lsi10G001710 vs. Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 261.9 bits (668), Expect = 8.4e-69
Identity = 136/293 (46.42%), Postives = 201/293 (68.60%), Query Frame = 1 

Query: 5   YAFLLLLLAFLLLQQLRTR-WRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIFT 64
           Y+   L L F+    +R R ++NLPP PPS+PIIG+LH L+RP HR F  ++ KYG +F+
Sbjct: 9   YSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFS 68

Query: 65  LRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLR 124
           L FGSRL ++VSS    Q+CFTKNDVV ANRPR L  +Y+ YN TT+ +  YG+HWRNLR
Sbjct: 69  LWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128

Query: 125 RLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVMRVV 184
           R+  +++ S  R+N+   IR+DE +RL+ +L  +S    A++EL S   +++FN +MR++
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMI 188

Query: 185 AGKRYYGE--EVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLT 244
           +GKRYYGE  + SD  EA +F +++ E+ +  GA+   DFMP+L+++ F+  EK L  ++
Sbjct: 189 SGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDIS 248

Query: 245 KRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVL 291
            + D F++ L+EE R KK    E  ++++  LL LQ S+PEYYTDQIIKG+ L
Sbjct: 249 GKTDAFLRGLIEEHRTKK----ERANTMIDHLLNLQDSQPEYYTDQIIKGLAL 297

[Close]
BLAST of Lsi10G001710 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.3e-66
Identity = 135/314 (42.99%), Postives = 205/314 (65.29%), Query Frame = 1 

Query: 5   YAFLLLLLAFL-----LLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYG 64
           + + LL L+F+     LL+    R +NLPP PP++PIIG+LH L+ P HR F  ++  YG
Sbjct: 3   FYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYG 62

Query: 65  PIFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHW 124
            IF+L FGSRL ++VSS  +A ECFTKND++ ANRPR L  +Y+ YN TT+ +A YGDHW
Sbjct: 63  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 122

Query: 125 RNLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSE------SLAKVELKSMFSELSF 184
           RNLRR+ T+++ S  RLN+ L +R+DE  RL+ KL  +         KVEL+   +E++F
Sbjct: 123 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 182

Query: 185 NIVMRVVAGKRYYGE--EVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYE 244
           N +MR+++GKRYYG+  +VSD  EA++F E++ E+    GA+   DF+P+L+ +  D  E
Sbjct: 183 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 242

Query: 245 KSLAKLTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIV- 301
           K   ++ KR++ F++ L+EE R   +    +  +++  LL+L  S+PEYY+D +IKG++ 
Sbjct: 243 KRCKRIAKRSNAFLEGLIEEHRRGNI--HSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 302

[Close]
BLAST of Lsi10G001710 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-65
Identity = 141/292 (48.29%), Postives = 194/292 (66.44%), Query Frame = 1 

Query: 7   FLLLLLAFLLLQQLRTRWRNLPPSPPS-VPIIGHLHLLQRPTHRNFHNIAAKYG--PIFT 66
           F  + L+  LL   R R  NLPPSP    PIIGHLHLL+ P HR F +++       IF+
Sbjct: 19  FFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFS 78

Query: 67  LRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLR 126
           L  GSRL  +VSS  +A+ECFTKNDVV ANRP  L+ +++GYN TTM  A YGD WRNLR
Sbjct: 79  LSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLR 138

Query: 127 RLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVMRVV 186
           R+ T+EIFS+ RLN+ + IR+DEIRRL++ L   S     KVE+K +F  L+ N ++R+V
Sbjct: 139 RIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMV 198

Query: 187 AGKRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKR 246
           AGKR+YG+   ++ EA+   +L+ E+   GGA    D+ PIL+++    YEK + KL  R
Sbjct: 199 AGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLAGR 258

Query: 247 ADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
            D F+Q LV E+R +KV    + ++++  LL LQ ++P+YYTD IIKGI+LV
Sbjct: 259 VDEFLQSLVNEKRVEKV----KGNTMIDHLLSLQETQPDYYTDVIIKGIILV 304

[Close]
BLAST of Lsi10G001710 vs. Swiss-Prot
Match: C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 5.7e-65
Identity = 130/293 (44.37%), Postives = 202/293 (68.94%), Query Frame = 1 

Query: 5   YAFLLLLLAFLLLQQLRTR-WRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIFT 64
           Y+   L + F++    ++R ++NLPP P S+PIIG+LH L+RP +R F  +  KYG + +
Sbjct: 9   YSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVIS 68

Query: 65  LRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLR 124
           L FGSRL ++VSSL   QECFTKNDVV ANRPR L  +Y+ YN TT+ +  YG+HWRNLR
Sbjct: 69  LWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128

Query: 125 RLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVMRVV 184
           R+ ++++ S  R+N    IR+DE +RL+ KL  +S    A+VEL   F +++FN +MR++
Sbjct: 129 RITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMI 188

Query: 185 AGKRYYGE--EVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLT 244
           +GKRYYG+  ++S+  EA +F +++ E+ +  GA+   DFMP+LK++ F+  EK + ++ 
Sbjct: 189 SGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIG 248

Query: 245 KRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVL 291
           ++ D F+  L++E+R+KK    E  ++++  LL +Q S+PEYYTD IIKG+ L
Sbjct: 249 EKNDVFLSGLLQEQRSKK----ERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 297

[Close]
BLAST of Lsi10G001710 vs. TrEMBL
Match: K7NC01_SIRGR (Cytochrome P450 OS=Siraitia grosvenorii GN=P450-1 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 9.2e-123
Identity = 229/295 (77.63%), Postives = 260/295 (88.14%), Query Frame = 1 

Query: 1   MDSRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGP 60
           MD   AFLLLLL  L+L Q+RTR RNLPPSPPS+PIIGHLHLL+RP HRNFH IAA+YGP
Sbjct: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60

Query: 61  IFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWR 120
           IF+LRFGSRLA+IVSSL IA+ECFTKND++FANRPRLLIS++LGYNCTTM+T+PYGDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120

Query: 121 NLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVM 180
           NLRRLA +EIFSTARLN+SL IRKDEI+RLL+KLHS S     KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180

Query: 181 RVVAGKRYYGEEVSDEAEAREFE-LMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKL 240
           R+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS W+DFMPILKWIG  G+EKSL +L
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240

Query: 241 TKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           TKR D+FMQ L+EERRNKKV   E ++SLL RLLELQ SEPEYYTDQIIKG+VLV
Sbjct: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLV 293

[Close]
BLAST of Lsi10G001710 vs. TrEMBL
Match: A0A061EFX4_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019137 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.0e-86
Identity = 166/296 (56.08%), Postives = 225/296 (76.01%), Query Frame = 1 

Query: 3   SRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIF 62
           S  + +LLL  F LL Q++T  +NLPPSPPS+P +GHLHL++RP HR +H+++ KYGPIF
Sbjct: 10  SSLSLILLLFCFKLLFQIKTSKQNLPPSPPSLPFLGHLHLIKRPIHRFYHSLSQKYGPIF 69

Query: 63  TLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNL 122
           +LRFGSRL ++VSS   A ECFTKND+V ANRP+LLI +YLGYN +T+  +PYGDHWRNL
Sbjct: 70  SLRFGSRLVVVVSSPTAAGECFTKNDIVLANRPKLLIGKYLGYNWSTVVGSPYGDHWRNL 129

Query: 123 RRLATVEIFSTARLNASLCIRKDEIRRLLVKLHS-----ESLAKVELKSMFSELSFNIVM 182
           RR++ +EIFS++RLNA L IRKDE++RLL+KL S     +   KVEL+S+F +L+FN +M
Sbjct: 130 RRISAIEIFSSSRLNAFLSIRKDEVKRLLLKLSSDDNSGQEFTKVELRSLFVDLTFNNIM 189

Query: 183 RVVAGKRYY-GEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAK 242
           R+VAGKRYY G+ V+DE EA+EF ELM+E    GG +   DF+PIL WIG   Y K L  
Sbjct: 190 RMVAGKRYYGGDSVTDEGEAKEFRELMKESLASGGVAHPGDFLPILNWIGGRSYLKKLMN 249

Query: 243 LTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           L +R DRF+Q+L++E R     A+ + ++++  LL LQ +EP+YYTDQ+IKG++LV
Sbjct: 250 LGERMDRFLQKLIDEVR-----AKRQGNTMIDHLLSLQQTEPDYYTDQLIKGLILV 300

[Close]
BLAST of Lsi10G001710 vs. TrEMBL
Match: A0A061EHP2_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019147 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.2e-84
Identity = 163/296 (55.07%), Postives = 222/296 (75.00%), Query Frame = 1 

Query: 3   SRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIF 62
           S  + +LLL  F LL Q++T  +NLPPSPP +P +GHLHL++RP HR +H+++ KYGPIF
Sbjct: 52  SSLSLILLLFCFKLLFQIKTSKQNLPPSPPPLPFLGHLHLIKRPIHRFYHSLSQKYGPIF 111

Query: 63  TLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNL 122
           +LRFGSRL ++VSS   A+ECFTKND+V A RP+LLI +YLGYN +T+  +PYGDHWRNL
Sbjct: 112 SLRFGSRLVVVVSSSTAAEECFTKNDIVLAYRPKLLIGKYLGYNWSTVVASPYGDHWRNL 171

Query: 123 RRLATVEIFSTARLNASLCIRKDEIRRLLVKLHS-----ESLAKVELKSMFSELSFNIVM 182
           RR+  +EIFS++RLNA L IRKDE++RLL+KL S     +   KVELKS+F +L+FN +M
Sbjct: 172 RRIGAIEIFSSSRLNAFLSIRKDEVKRLLMKLSSDDNSGQEFTKVELKSLFVDLTFNNIM 231

Query: 183 RVVAGKRYY-GEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAK 242
           R+VAGKRY+ G+ V+DE EA+EF ELM+E    GG +   DF+PIL WIG   Y K L  
Sbjct: 232 RMVAGKRYHGGDSVTDEGEAKEFRELMKESFASGGVAHPGDFLPILNWIGGRSYLKKLMN 291

Query: 243 LTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           L +R DR +Q+L++E R     A+ + ++++  LL LQ +EP+YYTDQ+IKG++LV
Sbjct: 292 LGERMDRLLQKLIDEVR-----AKRQGNTMIDHLLSLQQTEPDYYTDQLIKGLILV 342

[Close]
BLAST of Lsi10G001710 vs. TrEMBL
Match: A0A061EFX9_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019144 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.9e-84
Identity = 161/293 (54.95%), Postives = 220/293 (75.09%), Query Frame = 1 

Query: 3   SRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIF 62
           S  + +LLL++     Q +   +NLPPSPPS+PI+GHLHLL+   HR++H ++ KYGPIF
Sbjct: 9   SSLSLVLLLISLNFFFQSKKSHKNLPPSPPSLPILGHLHLLKPLIHRSYHTLSQKYGPIF 68

Query: 63  TLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNL 122
           +++ GSRL ++VSS   A+ECFTKND++ ANRP+L+  ++LGYN TT+  +PYGDHWRNL
Sbjct: 69  SIQLGSRLQVVVSSSAAAEECFTKNDIILANRPKLIQGKHLGYNYTTLIASPYGDHWRNL 128

Query: 123 RRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVMRV 182
           RR+  +EIFS++RLN S+ +R+DEIRRLL+KL  +S     KVELKSM SEL+FN +MR+
Sbjct: 129 RRIGAIEIFSSSRLNTSISVRRDEIRRLLLKLSRDSRQHFTKVELKSMLSELTFNNIMRM 188

Query: 183 VAGKRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTK 242
           VAGKRYYG+EV++EAEA+EF +L+ E+ +HGGA+   DF P+L W G   YEK   KL K
Sbjct: 189 VAGKRYYGDEVTNEAEAKEFRDLIAEVVKHGGAANPADFFPMLSWFG-QWYEKKDKKLGK 248

Query: 243 RADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           R D F Q+L++E R+     R+E +S++  LL LQ SEP YYTD I+KG+VLV
Sbjct: 249 RMDVFFQKLIDEHRS----YRQENTSMIAHLLSLQESEPHYYTDDILKGLVLV 296

[Close]
BLAST of Lsi10G001710 vs. TrEMBL
Match: A0A061EGZ3_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8 OS=Theobroma cacao GN=TCM_019148 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.5e-84
Identity = 162/289 (56.06%), Postives = 222/289 (76.82%), Query Frame = 1 

Query: 7   FLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIFTLRF 66
           FLL+ L F    Q +   +NLPPSPPS PI+GHLHLL+ P HR++H ++ KYGPIF+L+ 
Sbjct: 15  FLLISLNFFF--QSKKSHKNLPPSPPSRPILGHLHLLKPPIHRSYHALSQKYGPIFSLQL 74

Query: 67  GSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLRRLA 126
           GSRL ++VSS   A+ECFTKND++ ANRPRL+  ++LGYN TT+S +PYGDHWRNLRR+ 
Sbjct: 75  GSRLQVVVSSSAAAEECFTKNDIILANRPRLIRGKHLGYNYTTVSASPYGDHWRNLRRIG 134

Query: 127 TVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVMRVVAGK 186
            +EIFS++RLN ++ +R+DEIRRLL+KL  +S     KVELKSM SEL+FN +MR+VAGK
Sbjct: 135 AIEIFSSSRLNTTISVRRDEIRRLLLKLSRDSRQHFTKVELKSMLSELTFNNIMRMVAGK 194

Query: 187 RYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADR 246
           RYYG++V++EAEA+EF +L+ E+ ++GGA+   DF P+L WIG   YEK   KL +R D 
Sbjct: 195 RYYGDQVTNEAEAKEFWDLIAEVFKNGGAANPADFFPMLNWIG-QWYEKMDKKLGQRMDV 254

Query: 247 FMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           F Q+L++E R+    +R+E +S++  LL LQ SEP YYTD+I+KG++LV
Sbjct: 255 FFQKLIDEHRS----SRQENTSMIAHLLSLQESEPHYYTDEILKGLMLV 296

[Close]
BLAST of Lsi10G001710 vs. TAIR10
Match: AT2G23220.1 (AT2G23220.1 cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 265.8 bits (678), Expect = 3.3e-71
Identity = 146/298 (48.99%), Postives = 203/298 (68.12%), Query Frame = 1 

Query: 7   FLLLLLAFLLLQQLRTRWR------NLPPSPPS-VPIIGHLHLLQRPTHRNFHNIAAKYG 66
           FLLL LAF L   ++  +       NLPPSP   +P+IGHLHLL+ P HR F + +   G
Sbjct: 20  FLLLSLAFFLFISVKLLFGKRLSKFNLPPSPARPLPLIGHLHLLKLPLHRTFLSFSQSLG 79

Query: 67  --PIFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGD 126
             PIF LR G+RL ++VSS  IA+ECFTKND+VFANRP L++ +++ YN TTM++APYGD
Sbjct: 80  GAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAPYGD 139

Query: 127 HWRNLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFN 186
           HWRNLRR+AT+EIF++ +LN  L +RKDEIR+LL++L   S    AKVE++ +  EL+ N
Sbjct: 140 HWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFELTIN 199

Query: 187 IVMRVVAGKRYYGEEV-SDEAEAREFELMEEISRHGGASQWIDFMPILKWIGFDGYEKSL 246
            V R+VAGKR+YGE    DE   +   LM+EI    GA    D++PI++W  F  +EK +
Sbjct: 200 NVFRMVAGKRFYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRW--FTNFEKRV 259

Query: 247 AKLTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
             L  R D+F+Q LV+E+R  K    E+ ++++  LL LQ S+P+YYTD  +KGI++V
Sbjct: 260 KNLAIRIDKFLQSLVDEKRADK----EKGTTMIDHLLSLQESQPDYYTDVTLKGIIIV 311

[Close]
BLAST of Lsi10G001710 vs. TAIR10
Match: AT2G23190.1 (AT2G23190.1 cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 261.9 bits (668), Expect = 4.7e-70
Identity = 141/298 (47.32%), Postives = 202/298 (67.79%), Query Frame = 1 

Query: 7   FLLLLLAFLLLQQLRTRWR------NLPPSPPS-VPIIGHLHLLQRPTHRNFHNIAAKYG 66
           FL+L LAFL    L+  +       NLPPSP   +P IGHLHLL++P HR F + +   G
Sbjct: 49  FLILSLAFLFFISLKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSFSQSLG 108

Query: 67  --PIFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGD 126
             PIF+LR G+ L ++VSS  IA+ECFTKND+V ANRP+ ++ +++ YN TTM++APYGD
Sbjct: 109 DAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSAPYGD 168

Query: 127 HWRNLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFN 186
           HWRNLRR+ T+EIFS+ +LN  L +RKDEIR LL++L   S    AKVE++ +F +L+ N
Sbjct: 169 HWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYDLTIN 228

Query: 187 IVMRVVAGKRYYGEEV-SDEAEAREFELMEEISRHGGASQWIDFMPILKWIGFDGYEKSL 246
            ++R+VAGKR+YGE    DE   R  +L++EI    G     D++PIL+WI    +EK +
Sbjct: 229 NILRMVAGKRFYGEGTEQDEVARRVTQLIDEIVYRAGVGNAADYIPILRWI--TDFEKGV 288

Query: 247 AKLTKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
            +L  R D F+Q LV+ERR  K    ++ ++++  LL LQ ++P+YYTD  +KGI++V
Sbjct: 289 KELASRVDEFLQSLVDERRVHK----QKGNTMMDHLLSLQETQPDYYTDVTLKGIIIV 340

[Close]
BLAST of Lsi10G001710 vs. TAIR10
Match: AT4G37320.1 (AT4G37320.1 cytochrome P450, family 81, subfamily D, polypeptide 5)

HSP 1 Score: 257.7 bits (657), Expect = 9.0e-69
Identity = 147/304 (48.36%), Postives = 199/304 (65.46%), Query Frame = 1 

Query: 6   AFLLLLLAFLLLQQLRTRWRNLPPSPP-SVPIIGHLHLLQRPTHRNFHNIAAKYG--PIF 65
           +FL L ++  LL     R  NLPPSP   +P+IGHLHLL++P HR FH+I+   G  PIF
Sbjct: 10  SFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIF 69

Query: 66  TLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNL 125
            LR G+RL  ++SS  IA+ECFTKNDVV ANRP +++++++GYN T M  A YGDHWRNL
Sbjct: 70  HLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNL 129

Query: 126 RRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSESL---AKVELKSMFSELSFNIVMRV 185
           RR+A VEIFS+ R++    IRKDEIRRL+  L  +SL    +VELKS+ + L+FN ++ +
Sbjct: 130 RRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMM 189

Query: 186 VAGKRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTK 245
           VAGKRYYG    D  EA+   EL+ EI    G+    D++P + W+    +E     L  
Sbjct: 190 VAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILGN 249

Query: 246 RADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIV--LVSNPTS 301
           R DR +Q+LV+E+R +K    E+  +L+  LL  Q +EPEYYTD IIKGI+  LV   T 
Sbjct: 250 RLDRVLQKLVDEKRAEK----EKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTD 307

[Close]
BLAST of Lsi10G001710 vs. TAIR10
Match: AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 254.6 bits (649), Expect = 7.6e-68
Identity = 140/307 (45.60%), Postives = 207/307 (67.43%), Query Frame = 1 

Query: 5   YAFLLLLLAFLLLQQLRTRWRNLPPSPP-SVPIIGHLHLLQRPTHRNFHNIAAKYG--PI 64
           ++ L ++L+ + L     R  NLPPSP  S+P+IGHL LL+ P HR F +++      PI
Sbjct: 8   FSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPI 67

Query: 65  FTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRN 124
           F+LR G+RL  + SS  IA+ECFTKNDVV ANRP  ++++++ Y+ TTM  A YGDHWRN
Sbjct: 68  FSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRN 127

Query: 125 LRRLATVEIFSTARLNASLCIRKDEIRRLLVKL---HSESLAKVELKSMFSELSFNIVMR 184
           LRR+ +VEIFS  RLN+ L IRKDEIRRL+ +L    S+   KV++KSM S+L+FN ++R
Sbjct: 128 LRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILR 187

Query: 185 VVAGKRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLT 244
           +VAGKRYYG+ V D+ EA+   +L+ ++    GA   +D++P+L+ +    YE  + KL 
Sbjct: 188 MVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLA 247

Query: 245 KRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKG----IVLVSN 301
            R D F+Q LV+E+R     A+E+ ++++  LL LQ S+P+Y+TD+IIKG    ++L   
Sbjct: 248 GRLDEFLQGLVDEKRE----AKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 307

[Close]
BLAST of Lsi10G001710 vs. TAIR10
Match: AT4G37330.1 (AT4G37330.1 cytochrome P450, family 81, subfamily D, polypeptide 4)

HSP 1 Score: 253.1 bits (645), Expect = 2.2e-67
Identity = 143/291 (49.14%), Postives = 198/291 (68.04%), Query Frame = 1 

Query: 6   AFLLLLLAFLLLQQLRTRWRNLPPSPP-SVPIIGHLHLLQRPTHRNFHNIAAKYG--PIF 65
           +FL L    + L     R  NLPPSP  S+P+IGHLHLL+ P HR F +++   G  P+F
Sbjct: 10  SFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVF 69

Query: 66  TLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNL 125
            LR G+RL  ++SS  IA+ECFTKNDVV ANRP+  IS++LGYN T + +A YGDHWRNL
Sbjct: 70  HLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNL 129

Query: 126 RRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSESL---AKVELKSMFSELSFNIVMRV 185
           RR+A VEIFST RLN+ L IRKDEIRRL+  L  +SL    +VE+K++ + L+ N  +R+
Sbjct: 130 RRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRM 189

Query: 186 VAGKRYYGEEVSDEAEAREFELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKR 245
           +AGKRY+GE+ +D+A+  +  L+ E     GA   ID++ IL+W+    YEK +  L  R
Sbjct: 190 LAGKRYFGED-NDDAKLVK-NLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLGNR 249

Query: 246 ADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVL 291
            D F+Q+LV+E+R +K    E+  +++  LL LQ  +P+YYTD IIKGI+L
Sbjct: 250 FDTFLQKLVDEKRAEK----EKGETMIDHLLALQDIQPDYYTDVIIKGIIL 292

[Close]
BLAST of Lsi10G001710 vs. NCBI nr
Match: gi|659111597|ref|XP_008455812.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 461.1 bits (1185), Expect = 1.5e-126
Identity = 237/292 (81.16%), Postives = 256/292 (87.67%), Query Frame = 1 

Query: 1   MDSRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGP 60
           MD   AF LL L+FLLLQQLRTR RNLPPSPPS PIIGHLHLLQRPTHRNF NIAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWR 120
           IF+LRFGSRLA+IVSSLQIAQECFTK+D++FANRPRLL  +Y GY+ TTM+TA YGDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSESLAKVELKSMFSELSFNIVMRVV 180
           NLRRLA +EIFS  RLNASL  RKDEIRRLLVKLHSES AKVEL  MFSELSFNIVMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKR 240
           AGKRYYGE+VSDEAEAREF ELM+E+SR GG SQW+DFMPILKWIGF  YEK L K    
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           ADRF+QEL+EE RNKKV  REE+SSLL RLLELQLS+PEYYTDQIIKG+VLV
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLV 292

[Close]
BLAST of Lsi10G001710 vs. NCBI nr
Match: gi|449468317|ref|XP_004151868.1| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 459.9 bits (1182), Expect = 3.4e-126
Identity = 236/292 (80.82%), Postives = 258/292 (88.36%), Query Frame = 1 

Query: 1   MDSRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGP 60
           MD   +F LLLLAFLLLQQLRTR RNLPPSPPS PIIGHLHLLQRPTHRNF NIAAKYGP
Sbjct: 1   MDFLSSFFLLLLAFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWR 120
           IFTLRFGS LA+IVSSLQIAQECFTK+D++FANRPRLL  +YLGY+ TTM+ A YGDHWR
Sbjct: 61  IFTLRFGSHLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYLGYSWTTMAAASYGDHWR 120

Query: 121 NLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSESLAKVELKSMFSELSFNIVMRVV 180
           NLRRLA +EIFS  RLNASL  RKDEI+RLLVKLHSES AKVEL  MFSELSFNIVMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIQRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 AGKRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKR 240
           AGKRYYGE+VSD+AEAR+F ELM+E+SR GG SQW+DFMPI+KWIGF GYEK LAK    
Sbjct: 181 AGKRYYGEKVSDDAEARKFRELMDEVSRQGGTSQWVDFMPIMKWIGFGGYEKILAKSAIW 240

Query: 241 ADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           ADRF+QELV+E RNKKV  REE+SSLL RLLELQLS+PEYYTDQIIKG+VLV
Sbjct: 241 ADRFVQELVDEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLV 292

[Close]
BLAST of Lsi10G001710 vs. NCBI nr
Match: gi|343466197|gb|AEM42992.1| (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 448.0 bits (1151), Expect = 1.3e-122
Identity = 229/295 (77.63%), Postives = 260/295 (88.14%), Query Frame = 1 

Query: 1   MDSRYAFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGP 60
           MD   AFLLLLL  L+L Q+RTR RNLPPSPPS+PIIGHLHLL+RP HRNFH IAA+YGP
Sbjct: 1   MDFFSAFLLLLLTVLILLQIRTRRRNLPPSPPSLPIIGHLHLLKRPIHRNFHKIAAEYGP 60

Query: 61  IFTLRFGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWR 120
           IF+LRFGSRLA+IVSSL IA+ECFTKND++FANRPRLLIS++LGYNCTTM+T+PYGDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLDIAEECFTKNDLIFANRPRLLISKHLGYNCTTMATSPYGDHWR 120

Query: 121 NLRRLATVEIFSTARLNASLCIRKDEIRRLLVKLHSES---LAKVELKSMFSELSFNIVM 180
           NLRRLA +EIFSTARLN+SL IRKDEI+RLL+KLHS S     KVELK+MFSEL+FN +M
Sbjct: 121 NLRRLAAIEIFSTARLNSSLSIRKDEIQRLLLKLHSGSSGEFTKVELKTMFSELAFNALM 180

Query: 181 RVVAGKRYYGEEVSDEAEAREFE-LMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKL 240
           R+VAGKRYYG+EVSDE EAREF  LMEEIS HGGAS W+DFMPILKWIG  G+EKSL +L
Sbjct: 181 RIVAGKRYYGDEVSDEEEAREFRGLMEEISLHGGASHWVDFMPILKWIGGGGFEKSLVRL 240

Query: 241 TKRADRFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           TKR D+FMQ L+EERRNKKV   E ++SLL RLLELQ SEPEYYTDQIIKG+VLV
Sbjct: 241 TKRTDKFMQALIEERRNKKVL--ERKNSLLDRLLELQASEPEYYTDQIIKGLVLV 293

[Close]
BLAST of Lsi10G001710 vs. NCBI nr
Match: gi|1009171679|ref|XP_015866869.1| (PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba])

HSP 1 Score: 328.9 bits (842), Expect = 9.0e-87
Identity = 162/290 (55.86%), Postives = 217/290 (74.83%), Query Frame = 1 

Query: 6   AFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIFTLR 65
           +F++ L AF    + +T ++NLPP PPS+PI+GHL+LL+ P HR FH ++ KYGPIF+L 
Sbjct: 11  SFIIFLFAFKFFTKAKTPYKNLPPGPPSLPIVGHLYLLKPPVHRTFHRLSQKYGPIFSLW 70

Query: 66  FGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLRRL 125
           FGS   ++VSS     ECFTKND+V ANRP+L+  +++ YN TTM  APYGDHWRNLRR+
Sbjct: 71  FGSHRVVVVSSATAVHECFTKNDIVLANRPQLVRGKHVAYNYTTMVAAPYGDHWRNLRRI 130

Query: 126 ATVEIFSTARLNASLCIRKDEIRRLLVKLHSESL---AKVELKSMFSELSFNIVMRVVAG 185
            ++EIFS + LN  L IRKDE++RLL KL   S+    +VE+KSM  EL+FNI+MR+VAG
Sbjct: 131 GSIEIFSASLLNMFLSIRKDEVKRLLFKLSQNSVQDFKRVEMKSMLLELTFNIIMRMVAG 190

Query: 186 KRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRAD 245
           KRYYG+EVSDE EAR+F ELM+EI   GG S   DF+PIL W+G DG+E+S  +L KR+D
Sbjct: 191 KRYYGDEVSDEEEARQFRELMKEIFETGGVSNPGDFLPILNWLGIDGFERSTKRLAKRSD 250

Query: 246 RFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           +F+Q L++E RN K    E  ++++  LL LQ S+PEYYTD+IIKG++LV
Sbjct: 251 QFLQGLIDEHRNSK----ESRNTMIDHLLSLQQSQPEYYTDKIIKGLMLV 296

[Close]
BLAST of Lsi10G001710 vs. NCBI nr
Match: gi|1009174369|ref|XP_015868312.1| (PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba])

HSP 1 Score: 328.9 bits (842), Expect = 9.0e-87
Identity = 162/290 (55.86%), Postives = 217/290 (74.83%), Query Frame = 1 

Query: 6   AFLLLLLAFLLLQQLRTRWRNLPPSPPSVPIIGHLHLLQRPTHRNFHNIAAKYGPIFTLR 65
           +F++ L AF    + +T ++NLPP PPS+PI+GHL+LL+ P HR FH ++ KYGPIF+L 
Sbjct: 74  SFIIFLFAFKFFTKAKTPYKNLPPGPPSLPIVGHLYLLKPPVHRTFHRLSQKYGPIFSLW 133

Query: 66  FGSRLALIVSSLQIAQECFTKNDVVFANRPRLLISQYLGYNCTTMSTAPYGDHWRNLRRL 125
           FGS   ++VSS     ECFTKND+V ANRP+L+  +++ YN TTM  APYGDHWRNLRR+
Sbjct: 134 FGSHRVVVVSSATAVHECFTKNDIVLANRPQLVRGKHVAYNYTTMVAAPYGDHWRNLRRI 193

Query: 126 ATVEIFSTARLNASLCIRKDEIRRLLVKLHSESL---AKVELKSMFSELSFNIVMRVVAG 185
            ++EIFS + LN  L IRKDE++RLL KL   S+    +VE+KSM  EL+FNI+MR+VAG
Sbjct: 194 GSIEIFSASLLNMFLSIRKDEVKRLLFKLSQNSVQDFKRVEMKSMLLELTFNIIMRMVAG 253

Query: 186 KRYYGEEVSDEAEAREF-ELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRAD 245
           KRYYG+EVSDE EAR+F ELM+EI   GG S   DF+PIL W+G DG+E+S  +L KR+D
Sbjct: 254 KRYYGDEVSDEEEARQFRELMKEIFETGGVSNPGDFLPILNWLGIDGFERSTKRLAKRSD 313

Query: 246 RFMQELVEERRNKKVFAREEESSLLQRLLELQLSEPEYYTDQIIKGIVLV 292
           +F+Q L++E RN K    E  ++++  LL LQ S+PEYYTD+IIKG++LV
Sbjct: 314 QFLQGLIDEHRNSK----ESRNTMIDHLLSLQQSQPEYYTDKIIKGLMLV 359

The following BLAST results are available for this feature:
BLAST of Lsi10G001710 vs. Swiss-Prot
Match NameE-valueIdentityDescription
C81E8_MEDTR1.3e-7750.33Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1[more]
C81E1_GLYEC8.4e-6946.42Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2[more]
C81E9_MEDTR1.3e-6642.99Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1[more]
C8D11_ARATH1.9e-6548.29Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1[more]
C81E7_MEDTR5.7e-6544.37Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1[more]
BLAST of Lsi10G001710 vs. TrEMBL
Match NameE-valueIdentityDescription
K7NC01_SIRGR9.2e-12377.63Cytochrome P450 OS=Siraitia grosvenorii GN=P450-1 PE=2 SV=1[more]
A0A061EFX4_THECC4.0e-8656.08Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... [more]
A0A061EHP2_THECC2.2e-8455.07Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... [more]
A0A061EFX9_THECC2.9e-8454.95Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... [more]
A0A061EGZ3_THECC6.5e-8456.06Cytochrome P450, family 81, subfamily D, polypeptide 8 OS=Theobroma cacao GN=TCM... [more]
BLAST of Lsi10G001710 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G23220.13.3e-7148.99 cytochrome P450, family 81, subfamily D, polypeptide 6[more]
AT2G23190.14.7e-7047.32 cytochrome P450, family 81, subfamily D, polypeptide 7[more]
AT4G37320.19.0e-6948.36 cytochrome P450, family 81, subfamily D, polypeptide 5[more]
AT4G37370.17.6e-6845.60 cytochrome P450, family 81, subfamily D, polypeptide 8[more]
AT4G37330.12.2e-6749.14 cytochrome P450, family 81, subfamily D, polypeptide 4[more]
BLAST of Lsi10G001710 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659111597|ref|XP_008455812.1|1.5e-12681.16PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo][more]
gi|449468317|ref|XP_004151868.1|3.4e-12680.82PREDICTED: cytochrome P450 81E8-like [Cucumis sativus][more]
gi|343466197|gb|AEM42992.1|1.3e-12277.63cytochrome P450 [Siraitia grosvenorii][more]
gi|1009171679|ref|XP_015866869.1|9.0e-8755.86PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba][more]
gi|1009174369|ref|XP_015868312.1|9.0e-8755.86PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G001710.1Lsi10G001710.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 19..290
score: 1.1
IPR001128Cytochrome P450PFAMPF00067p450coord: 28..287
score: 4.3
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 28..290
score: 7.73
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 171..189
score: 3.7E-10coord: 79..100
score: 3.7E-10coord: 55..74
score: 3.7
NoneNo IPR availableunknownCoilCoilcoord: 226..253
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..291
score: 3.3E
NoneNo IPR availablePANTHERPTHR24298:SF61CYTOCHROME P450 81D1-RELATEDcoord: 1..291
score: 3.3E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi10G001710ClCG02G008220Watermelon (Charleston Gray)lsiwcgB055
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi10G001710Cucumber (Gy14) v1cgylsiB071
Lsi10G001710Cucurbita maxima (Rimu)cmalsiB507
Lsi10G001710Cucurbita maxima (Rimu)cmalsiB548
Lsi10G001710Cucurbita moschata (Rifu)cmolsiB491
Lsi10G001710Cucurbita moschata (Rifu)cmolsiB533
Lsi10G001710Cucurbita pepo (Zucchini)cpelsiB220
Lsi10G001710Cucurbita pepo (Zucchini)cpelsiB589
Lsi10G001710Wild cucumber (PI 183967)cpilsiB092
Lsi10G001710Cucumber (Chinese Long) v2culsiB094
Lsi10G001710Melon (DHL92) v3.5.1lsimeB053
Lsi10G001710Watermelon (97103) v1lsiwmB068
Lsi10G001710Cucumber (Gy14) v2cgyblsiB087
Lsi10G001710Melon (DHL92) v3.6.1lsimedB054
Lsi10G001710Silver-seed gourdcarlsiB374
Lsi10G001710Silver-seed gourdcarlsiB870
Lsi10G001710Cucumber (Chinese Long) v3cuclsiB105
Lsi10G001710Cucumber (Chinese Long) v3cuclsiB449
Lsi10G001710Watermelon (97103) v2lsiwmbB054
Lsi10G001710Wax gourdlsiwgoB085
Lsi10G001710Wax gourdlsiwgoB069