ClCG02G008220 (gene) Watermelon (Charleston Gray)

Overview
NameClCG02G008220
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionCytochrome P450
LocationCG_Chr02 : 10461207 .. 10463307 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCATGTGGGCAAAAGGCACAGCATCTATATCAATAAGTCCACAAAAGCCAAATTTCGATTGAAACTATGGAATAAACCAAATGGATTCTCTCAACGCTCTCTTTCTCCTTCTTGCTTTCCTTTTACTGCTACAACTCCATTCGGAATCGTTCGCTAAGGTGGAGTTGAAATCGATGTTTTCGGAACTGAGTTTCAACACCGTGATGAGGGTCGTGGCCGGAAAACGGTATTACGGCGAGGAGGTTTCCGATGAGGCGGAGGCGAGGGAGTTCAGAGAGCTTATGGAGGAGATTTCTCGCCATGGAGGAGCTTCTCAATGGATCGATTTCATGCCGATTTTGAAATGGATTGGATTTGACGGATATGAGAAGAGTTTGGCCAAATTGACGAAAAGGGCCGATCGATTTATGCAGGAATTGGTTGAAGAGCGGCGGAATAAGAAAGTGTTGGCAAAAGAAGAACAGAGTTCTCTGCTTCATCGGCTTCTTGAATTGCAGCTCTCTGAACCCGAATACTACACCGATCAGATCATCAAAGGGATCGTTCTGGTTAGTAATCCAACCTTCCACTTATTCAACCTAATCGGTTTTTGTTTTTCATTCTTTGGTTTCTTTGAATTTTCGTAAGTGGGATACTGAAACAGAGTCCATCTAAATTGAATGTTGTAAACTCCATGAACGACTCATCGCAGAAACCAAAAGGGGAATGGAAGAATCTGGTTTACTTAACTCTTTAATTCTTTAACTTAGTCAAACATCGGCAAACTTTAACACTTCTATTTATTTTGGTCTCTATAATTTCATCTAGTCTACTTTTAGTTTTAAATATTTGAACTTTTAAAAAATTTCATTTAACTTTTAGGCTTTTGTCCTAAAATTTATGAACTAAAAAGTTTATGATTCCATATTTATTGTTGGAGTGAACAAATCTAAACAAATAGGATTTAGAGGTAGTAAATATAGTCCAGTTAACAAAGAAAAATAAAAAATAAAAGAAAAGAAAATTTGAGGCGTAAAATTGCAAGGAGGAGATAACAACGAGTATGGTTTACAAATAGGTCATATTTATATTTTGTTCATGTAAATAAATACAAGCTTTTTAATCTATGAATTTTATGGCAAAAGCCTATTACAGTTGTTTCGAAACAAATTCTAAACTTCATAGGTTAAAATGAAATTTTTAAGAATTCAATATAATTAAAACCAACCTTATGTTAAAGATATTTTTGATAAATATAATCTTTAACCTATAACATTTCTATCTGTCCATTTTTCTTTCATTTTCTTCCCTCATAATTTTAGTATGAGACATCATATAATAAATTTCTCAAGTCTCACATTTGTATTAAAGATTTCCCGTAAGTATGAAAATTGTGTTAAGATCAAAATGGCCACTTTTGTAAGTTGTTTGAACGGTATGTGTTTGCTTCAACTTTTAAGCATTTAATCCACGTTCTTGTATAGTATAGGTTCTATTGCGTGCAGGAACAGACACATCATCTGTGACCTTGGATTGGGCCATGACTCAACTACTCAACCATCCGGAGGTATTAGTTAAAGCCAAAGCTGAGATAGACACCAAGATTGGTCAAGATCGACCCCTTGAGGAAGCAGATCTCAACAACCTTAACTACCTTCAAGCTATAATTTCTGAGACCTTTCGATTGCACCCCGCAGCACCGATGCTCCTCTCGCACTATTCCTCTGACGACTGTACTGTCGCAGGCTACAACATTCCACGTGGGACGACGTTGTTGGTCAATGCTTGGGCAATTCATAGAGACCCGAAGCTGTGGGACGACCCGACGAGCTTCCGACCGGAGAGATTCTTGGGGGCAGGGAATGGGTTGCAGTCCAATAAACTCATTCCTTTTGGAATTGGAAGGAGGGCTTGTCCTGGGGAAACTATGGCTTTACGTTTTATAGGATTGACTTTGGGGCTATTGATTCAGTGTTATGAGTGGAAAAAGGCTGGAGGATGTGAAAAGGTTGACATGACTGAAGGTGGAGGTATCACCATTCATAAAGTTAAGCCTTTAGAAACTATGTGTAAAGCTCGTCCAATTATGGATAAAGTTCTATCAAATGGTTTGGACTAA

mRNA sequence

CCCTCATGTGGGCAAAAGGCACAGCATCTATATCAATAAGTCCACAAAAGCCAAATTTCGATTGAAACTATGGAATAAACCAAATGGATTCTCTCAACGCTCTCTTTCTCCTTCTTGCTTTCCTTTTACTGCTACAACTCCATTCGGAATCGTTCGCTAAGGTGGAGTTGAAATCGATGTTTTCGGAACTGAGTTTCAACACCGTGATGAGGGTCGTGGCCGGAAAACGGTATTACGGCGAGGAGGTTTCCGATGAGGCGGAGGCGAGGGAGTTCAGAGAGCTTATGGAGGAGATTTCTCGCCATGGAGGAGCTTCTCAATGGATCGATTTCATGCCGATTTTGAAATGGATTGGATTTGACGGATATGAGAAGAGTTTGGCCAAATTGACGAAAAGGGCCGATCGATTTATGCAGGAATTGGTTGAAGAGCGGCGGAATAAGAAAGTGTTGGCAAAAGAAGAACAGAGTTCTCTGCTTCATCGGCTTCTTGAATTGCAGCTCTCTGAACCCGAATACTACACCGATCAGATCATCAAAGGGATCGTTCTGTCCAATAAACTCATTCCTTTTGGAATTGGAAGGAGGGCTTGTCCTGGGGAAACTATGGCTTTACGTTTTATAGGATTGACTTTGGGGCTATTGATTCAGTGTTATGAGTGGAAAAAGGCTGGAGGATGTGAAAAGGTTGACATGACTGAAGGTGGAGGTATCACCATTCATAAAGTTAAGCCTTTAGAAACTATGTGTAAAGCTCGTCCAATTATGGATAAAGTTCTATCAAATGGTTTGGACTAA

Coding sequence (CDS)

ATGGATTCTCTCAACGCTCTCTTTCTCCTTCTTGCTTTCCTTTTACTGCTACAACTCCATTCGGAATCGTTCGCTAAGGTGGAGTTGAAATCGATGTTTTCGGAACTGAGTTTCAACACCGTGATGAGGGTCGTGGCCGGAAAACGGTATTACGGCGAGGAGGTTTCCGATGAGGCGGAGGCGAGGGAGTTCAGAGAGCTTATGGAGGAGATTTCTCGCCATGGAGGAGCTTCTCAATGGATCGATTTCATGCCGATTTTGAAATGGATTGGATTTGACGGATATGAGAAGAGTTTGGCCAAATTGACGAAAAGGGCCGATCGATTTATGCAGGAATTGGTTGAAGAGCGGCGGAATAAGAAAGTGTTGGCAAAAGAAGAACAGAGTTCTCTGCTTCATCGGCTTCTTGAATTGCAGCTCTCTGAACCCGAATACTACACCGATCAGATCATCAAAGGGATCGTTCTGTCCAATAAACTCATTCCTTTTGGAATTGGAAGGAGGGCTTGTCCTGGGGAAACTATGGCTTTACGTTTTATAGGATTGACTTTGGGGCTATTGATTCAGTGTTATGAGTGGAAAAAGGCTGGAGGATGTGAAAAGGTTGACATGACTGAAGGTGGAGGTATCACCATTCATAAAGTTAAGCCTTTAGAAACTATGTGTAAAGCTCGTCCAATTATGGATAAAGTTCTATCAAATGGTTTGGACTAA

Protein sequence

MDSLNALFLLLAFLLLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLELQLSEPEYYTDQIIKGIVLSNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVKPLETMCKARPIMDKVLSNGLD
Homology
[Close]
BLAST of ClCG02G008220 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 9.4e-31
Identity = 69/143 (48.25%), Postives = 95/143 (66.43%), Query Frame = 1 

Query: 16  LLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGE--EVSDEAEAREFRELMEEISR 75
           L Q     F +VEL+ MFSE++FNT+MR+V+GKRYYG   +VSD  EAR FR +++E+  
Sbjct: 159 LAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVS 218

Query: 76  HGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLH 135
            GGA+   DF+  L+W  FDG EK L K++KR D F+Q L++E R      K   ++++ 
Sbjct: 219 LGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHR----FGKRNSNTMID 278

Query: 136 RLLELQLSEPEYYTDQIIKGIVL 157
            LL  Q S+PEYYTDQIIKG+++
Sbjct: 279 HLLTQQQSQPEYYTDQIIKGLMV 297


HSP 2 Score: 87.0 bits (214), Expect = 2.9e-16
Identity = 42/68 (61.76%), Postives = 47/68 (69.12%), Query Frame = 1 

Query: 158 NKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVKP 217
           NKL+ FG+GRRACPGE ++ R  GLTLGLLIQC+EWK+ G  EK+DM E  GIT  K   
Sbjct: 424 NKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGE-EKIDMVEAKGITAGKKTS 483

Query: 218 LETMCKAR 226
           L  MCK R
Sbjct: 484 LNAMCKVR 490

[Close]
BLAST of ClCG02G008220 vs. Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 9.7e-28
Identity = 61/139 (43.88%), Postives = 96/139 (69.06%), Query Frame = 1 

Query: 21  SESFAKVELKSMFSELSFNTVMRVVAGKRYYGEE--VSDEAEAREFRELMEEISRHGGAS 80
           S +FA++EL S   +++FN +MR+++GKRYYGE+   SD  EA +FR+++ E+ +  GA+
Sbjct: 164 STNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGAN 223

Query: 81  QWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLEL 140
              DFMP+L+++ F+  EK L  ++ + D F++ L+EE R K    KE  ++++  LL L
Sbjct: 224 NKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTK----KERANTMIDHLLNL 283

Query: 141 QLSEPEYYTDQIIKGIVLS 158
           Q S+PEYYTDQIIKG+ L+
Sbjct: 284 QDSQPEYYTDQIIKGLALA 298


HSP 2 Score: 92.0 bits (227), Expect = 9.1e-18
Identity = 41/73 (56.16%), Postives = 56/73 (76.71%), Query Frame = 1 

Query: 159 KLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVKPL 218
           KLI FG+GRRACPGE +A+R I +TL LLIQC++WK   G +K+D+ E  G T+ K+ PL
Sbjct: 425 KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLING-DKIDLAERDGFTLTKLVPL 484

Query: 219 ETMCKARPIMDKV 232
           + MCK+RP+++KV
Sbjct: 485 KAMCKSRPVINKV 496

[Close]
BLAST of ClCG02G008220 vs. Swiss-Prot
Match: C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.3e-26
Identity = 57/139 (41.01%), Postives = 96/139 (69.06%), Query Frame = 1 

Query: 21  SESFAKVELKSMFSELSFNTVMRVVAGKRYYGE--EVSDEAEAREFRELMEEISRHGGAS 80
           S  FA+VEL   F +++FN +MR+++GKRYYG+  ++S+  EA +FR+++ E+ +  GA+
Sbjct: 164 STKFAEVELTFRFFDMTFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGAN 223

Query: 81  QWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLEL 140
              DFMP+LK++ F+  EK + ++ ++ D F+  L++E+R+K    KE  ++++  LL +
Sbjct: 224 NKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSGLLQEQRSK----KERTNTMIDHLLNM 283

Query: 141 QLSEPEYYTDQIIKGIVLS 158
           Q S+PEYYTD IIKG+ L+
Sbjct: 284 QESQPEYYTDTIIKGLCLA 298


HSP 2 Score: 92.4 bits (228), Expect = 7.0e-18
Identity = 41/73 (56.16%), Postives = 56/73 (76.71%), Query Frame = 1 

Query: 159 KLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVKPL 218
           K+I FG+GRRACPGE +ALR I +TL LL+QC++WK+    EK+DM+E  G T+ K+ PL
Sbjct: 425 KMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRIND-EKIDMSERDGFTMTKLLPL 484

Query: 219 ETMCKARPIMDKV 232
           + MCK RP+++KV
Sbjct: 485 KAMCKTRPVVNKV 496

[Close]
BLAST of ClCG02G008220 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-24
Identity = 59/143 (41.26%), Postives = 90/143 (62.94%), Query Frame = 1 

Query: 14  LLLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISR 73
           + L +     F KVE+K +F  L+ N ++R+VAGKR+YG+   ++ EA+  R+L+ E+  
Sbjct: 167 ICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVV 226

Query: 74  HGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLH 133
            GGA    D+ PIL+++    YEK + KL  R D F+Q LV E+R +KV    + ++++ 
Sbjct: 227 SGGAGNAADYFPILRYV--TNYEKHVKKLAGRVDEFLQSLVNEKRVEKV----KGNTMID 286

Query: 134 RLLELQLSEPEYYTDQIIKGIVL 157
            LL LQ ++P+YYTD IIKGI+L
Sbjct: 287 HLLSLQETQPDYYTDVIIKGIIL 303


HSP 2 Score: 80.5 bits (197), Expect = 2.7e-14
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 1 

Query: 142 EPEYYTDQIIKGIVLSNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEK 201
           EPE +  +  +      KL+PFGIGRR+CPG  +A R + L LG L+QC+EW++    + 
Sbjct: 414 EPEKFKPERFEKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEE-KY 473

Query: 202 VDMTEG-GGITIHKVKPLETMCKARPIMDKVL 233
           +DM E   G T+ K   L+ MCKARPI+ KVL
Sbjct: 474 LDMRESEKGTTMRKATSLQAMCKARPIVHKVL 504

[Close]
BLAST of ClCG02G008220 vs. Swiss-Prot
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 56/130 (43.08%), Postives = 87/130 (66.92%), Query Frame = 1 

Query: 27  VELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHGGASQWIDFMPI 86
           VELK M  +L+FN +MR++ GKRYYGEE +DE EA+  R+L+ ++  +  +   +D++PI
Sbjct: 178 VELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI 237

Query: 87  LKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLELQLSEPEYY 146
           L+   F  YE  + KL +  D+F+Q L++++R +    +E  ++++  LL LQ S+ EYY
Sbjct: 238 LRL--FSSYENRVKKLGEETDKFLQGLIDDKRGQ----QETGTTMIDHLLVLQKSDIEYY 297

Query: 147 TDQIIKGIVL 157
           TDQIIKGI+L
Sbjct: 298 TDQIIKGIIL 301


HSP 2 Score: 92.4 bits (228), Expect = 7.0e-18
Identity = 42/77 (54.55%), Postives = 55/77 (71.43%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           + KL+ FG+GRRACPG  +A R +GL LG LIQC+EW++ G  E VDM EG G T+ K  
Sbjct: 427 AQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVE-VDMKEGVGNTVPKAI 486

Query: 217 PLETMCKARPIMDKVLS 234
           PL+ +CKARP + K++S
Sbjct: 487 PLKAICKARPFLHKIIS 502

[Close]
BLAST of ClCG02G008220 vs. TrEMBL
Match: A0A0A0LQK7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425760 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-58
Identity = 115/142 (80.99%), Postives = 129/142 (90.85%), Query Frame = 1 

Query: 15  LLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRH 74
           LL++LHSESFAKVEL  MFSELSFN VMRVVAGKRYYGE+VSD+AEAR+FRELM+E+SR 
Sbjct: 23  LLVKLHSESFAKVELTPMFSELSFNIVMRVVAGKRYYGEKVSDDAEARKFRELMDEVSRQ 82

Query: 75  GGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHR 134
           GG SQW+DFMPI+KWIGF GYEK LAK    ADRF+QELV+E RNKKVL +EEQSSLLHR
Sbjct: 83  GGTSQWVDFMPIMKWIGFGGYEKILAKSAIWADRFVQELVDEHRNKKVLGREEQSSLLHR 142

Query: 135 LLELQLSEPEYYTDQIIKGIVL 157
           LLELQLS+PEYYTDQIIKG+VL
Sbjct: 143 LLELQLSQPEYYTDQIIKGLVL 164

[Close]
BLAST of ClCG02G008220 vs. TrEMBL
Match: A0A0A0LQK7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425760 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.9e-26
Identity = 61/81 (75.31%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           +NKLIPFG+GRRACPGE M LR +GLTLGLLIQCYEWKK  G + VD TE GGITI KVK
Sbjct: 293 TNKLIPFGVGRRACPGEIMGLRVVGLTLGLLIQCYEWKK-HGYDNVDTTEYGGITILKVK 352

Query: 217 PLETMCKARPIMDKVLSNGLD 238
           P+ETMCK RP+M K+LSN LD
Sbjct: 353 PVETMCKPRPVMAKLLSNSLD 372


HSP 2 Score: 216.1 bits (549), Expect = 4.7e-53
Identity = 111/145 (76.55%), Postives = 125/145 (86.21%), Query Frame = 1 

Query: 15  LLLQLHSES---FAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEI 74
           LLL+LHS S   F KVELK+MFSEL+FN +MR+VAGKRYYG+EVSDE EAREFR LMEEI
Sbjct: 150 LLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEI 209

Query: 75  SRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSL 134
           S HGGAS W+DFMPILKWIG  G+EKSL +LTKR D+FMQ L+EERRNKKVL  E ++SL
Sbjct: 210 SLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKRTDKFMQALIEERRNKKVL--ERKNSL 269

Query: 135 LHRLLELQLSEPEYYTDQIIKGIVL 157
           L RLLELQ SEPEYYTDQIIKG+VL
Sbjct: 270 LDRLLELQASEPEYYTDQIIKGLVL 292

[Close]
BLAST of ClCG02G008220 vs. TrEMBL
Match: K7NC01_SIRGR (Cytochrome P450 OS=Siraitia grosvenorii GN=P450-1 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-27
Identity = 63/81 (77.78%), Postives = 69/81 (85.19%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           S KLI FG+GRR+CPG+TMALRF+GLTLGLLIQCY+WKK G  EKVDM EGGGITIHK K
Sbjct: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGD-EKVDMGEGGGITIHKAK 480

Query: 217 PLETMCKARPIMDKVLSNGLD 238
           PLE MCKARP M K+L N LD
Sbjct: 481 PLEAMCKARPAMYKLLLNALD 500


HSP 2 Score: 162.2 bits (409), Expect = 8.0e-37
Identity = 97/254 (38.19%), Postives = 137/254 (53.94%), Query Frame = 1 

Query: 21  SESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHGGASQW 80
           S S+ KVELKS+F  L FN ++R++AGKRY+GE V D  +A  F ++++EI     AS  
Sbjct: 167 SISYVKVELKSVFRVLVFNILLRMLAGKRYFGEGVVDVEDAERFTDIIKEILEFAAASNP 226

Query: 81  IDFMPILKWIGFDGYEKSLAKLTKRADRFMQ------------ELVEERRNKKVLAKEEQ 140
            DF+PIL WI + GY K + +L K+ D F+Q               EE    ++L  +E 
Sbjct: 227 ADFLPILGWIDYGGYIKRIKRLAKKTDTFLQGLVDQHRRVLSSSEDEESMINRLLKLQES 286

Query: 141 SS----------------------------LLHRLLELQLSEP-EYYTDQIIKGIVLSN- 200
                                          +HR    Q  EP ++  ++  KG V+   
Sbjct: 287 DPDYYTDETIKGLILLTDIPSETMLMVNAWAIHRDTH-QWDEPTQFKPERFEKGDVIDQY 346

Query: 201 KLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVKPL 233
           KL+PFG+GRRACPG  +  R +GL LG LIQC++WK+ G  ++VDM EG G+ + K  PL
Sbjct: 347 KLLPFGVGRRACPGANLGNRMVGLALGSLIQCFDWKRVGD-DEVDMAEGRGLNMPKAHPL 406

[Close]
BLAST of ClCG02G008220 vs. TrEMBL
Match: W9R6T6_9ROSA (Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009302 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.8e-34
Identity = 78/148 (52.70%), Postives = 104/148 (70.27%), Query Frame = 1 

Query: 9   LLLAFLLLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELM 68
           LL+      Q  ++ FAKVELKSMF  L+FN +MR+VAGKRYYGE V+DEAEA +FR++M
Sbjct: 156 LLIRIFPHSQRGTDDFAKVELKSMFVNLTFNMIMRMVAGKRYYGEGVTDEAEALQFRKIM 215

Query: 69  EEISRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQ 128
           +E    GG +   DF PIL W G  GYEK + +L K+ D F+Q L+EE R++    KE +
Sbjct: 216 KEAIDSGGLANPGDFFPILNWFGNGGYEKKVRRLAKQTDAFLQGLIEEHRSR----KESR 275

Query: 129 SSLLHRLLELQLSEPEYYTDQIIKGIVL 157
           ++++  LL LQ S+PEYYTDQIIKG +L
Sbjct: 276 NTMIDHLLSLQESQPEYYTDQIIKGFIL 299

[Close]
BLAST of ClCG02G008220 vs. TrEMBL
Match: W9R6T6_9ROSA (Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009302 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.4e-19
Identity = 49/77 (63.64%), Postives = 59/77 (76.62%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           +NK++PFG+GRRACPG  +A R +G TLG LIQC+EWK+    E+VDMTEG G T+ K  
Sbjct: 426 TNKMMPFGLGRRACPGAGLAQRVVGQTLGSLIQCFEWKRVNE-EEVDMTEGRGFTMPKAM 485

Query: 217 PLETMCKARPIMDKVLS 234
           PLE MCKARPIM  VLS
Sbjct: 486 PLEAMCKARPIMSTVLS 501


HSP 2 Score: 152.1 bits (383), Expect = 8.3e-34
Identity = 80/145 (55.17%), Postives = 106/145 (73.10%), Query Frame = 1 

Query: 15  LLLQLHSES---FAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEI 74
           LLL+L  +S   F KVELKSM SEL+FN +MR+VAGKRYYG+EV++EAEA+EFR+L+ E+
Sbjct: 156 LLLKLSRDSRQHFTKVELKSMLSELTFNNIMRMVAGKRYYGDEVTNEAEAKEFRDLIAEV 215

Query: 75  SRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSL 134
            +HGGA+   DF P+L W G   YEK   KL KR D F Q+L++E R+     ++E +S+
Sbjct: 216 VKHGGAANPADFFPMLSWFG-QWYEKKDKKLGKRMDVFFQKLIDEHRS----YRQENTSM 275

Query: 135 LHRLLELQLSEPEYYTDQIIKGIVL 157
           +  LL LQ SEP YYTD I+KG+VL
Sbjct: 276 IAHLLSLQESEPHYYTDDILKGLVL 295

[Close]
BLAST of ClCG02G008220 vs. TAIR10
Match: AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 124.8 bits (312), Expect = 7.2e-29
Identity = 67/137 (48.91%), Postives = 91/137 (66.42%), Query Frame = 1 

Query: 21  SESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHGGASQW 80
           S  F KVE+KSMFS L+FN ++R++AGK YYG+   D+ EA+  REL+ E     GA   
Sbjct: 165 SLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNT 224

Query: 81  IDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLELQL 140
            D++PIL WI   G EK + K+  R D F+Q LV+ERR  K   ++ Q++++  LL LQ 
Sbjct: 225 ADYLPILTWI--TGSEKRIKKIASRLDEFLQGLVDERREGK---EKRQNTMVDHLLCLQE 284

Query: 141 SEPEYYTDQIIKGIVLS 158
           ++PEYYTD IIKGI+LS
Sbjct: 285 TQPEYYTDNIIKGIMLS 296


HSP 2 Score: 90.1 bits (222), Expect = 2.0e-18
Identity = 43/78 (55.13%), Postives = 57/78 (73.08%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTE-GGGITIHKV 216
           + KL+ FG+GRRACPG  +A R   LT+G LIQC+EW++ G  E+VDMTE GGG+ + K 
Sbjct: 421 AQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGE-EEVDMTEGGGGVIMPKA 480

Query: 217 KPLETMCKARPIMDKVLS 234
            PL  MCKARP++ K+L+
Sbjct: 481 IPLVAMCKARPVVGKILN 497

[Close]
BLAST of ClCG02G008220 vs. TAIR10
Match: AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 122.9 bits (307), Expect = 2.7e-28
Identity = 60/137 (43.80%), Postives = 94/137 (68.61%), Query Frame = 1 

Query: 21  SESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHGGASQW 80
           S+ F KV++KSM S+L+FN ++R+VAGKRYYG+ V D+ EA+  R+L+ ++    GA   
Sbjct: 165 SQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNA 224

Query: 81  IDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLELQL 140
           +D++P+L+ +    YE  + KL  R D F+Q LV+E+R     AKE+ ++++  LL LQ 
Sbjct: 225 VDYLPVLRLV--SDYETRVKKLAGRLDEFLQGLVDEKRE----AKEKGNTMIDHLLTLQE 284

Query: 141 SEPEYYTDQIIKGIVLS 158
           S+P+Y+TD+IIKG +L+
Sbjct: 285 SQPDYFTDRIIKGNMLA 295


HSP 2 Score: 98.6 bits (244), Expect = 5.5e-21
Identity = 46/80 (57.50%), Postives = 58/80 (72.50%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           + KL+PFG+GRRACPG  +A R I LTLG LIQC EW+K G  E+VDM+EG G+T+ K K
Sbjct: 420 AQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG--EEVDMSEGKGVTMPKAK 479

Query: 217 PLETMCKARPIMDKVLSNGL 237
           PLE MC+ARP + K+ +  +
Sbjct: 480 PLEAMCRARPSVVKIFNESV 497

[Close]
BLAST of ClCG02G008220 vs. TAIR10
Match: AT2G23220.1 (AT2G23220.1 cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-26
Identity = 62/145 (42.76%), Postives = 91/145 (62.76%), Query Frame = 1 

Query: 15  LLLQLHSES---FAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEI 74
           LLL+L   S   FAKVE++ +  EL+ N V R+VAGKR+YGE    +  A++ R LM+EI
Sbjct: 172 LLLRLSKNSRHGFAKVEMRHLLFELTINNVFRMVAGKRFYGEGTEQDEVAQQVRHLMDEI 231

Query: 75  SRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSL 134
               GA    D++PI++W  F  +EK +  L  R D+F+Q LV+E+R      KE+ +++
Sbjct: 232 VTSAGAGNAADYLPIMRW--FTNFEKRVKNLAIRIDKFLQSLVDEKR----ADKEKGTTM 291

Query: 135 LHRLLELQLSEPEYYTDQIIKGIVL 157
           +  LL LQ S+P+YYTD  +KGI++
Sbjct: 292 IDHLLSLQESQPDYYTDVTLKGIII 310


HSP 2 Score: 80.5 bits (197), Expect = 1.5e-15
Identity = 40/92 (43.48%), Postives = 59/92 (64.13%), Query Frame = 1 

Query: 142 EPEYYTDQIIKGIVLSNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEK 201
           +PE +  +  K   L+ KL+ FG+GRRACPG  +A R + LTLG ++QC+EW++ G  E 
Sbjct: 421 DPEMFKPERFKNEKLNQKLLSFGMGRRACPGYGLAHRVVSLTLGSMVQCFEWQRIGQ-EY 480

Query: 202 VDMTEGGGITIHK-VKPLETMCKARPIMDKVL 233
           VD +E   + + +   PL  MCKARPI+ ++L
Sbjct: 481 VDNSEDKTVVLMRPTTPLLAMCKARPIVHEIL 511

[Close]
BLAST of ClCG02G008220 vs. TAIR10
Match: AT4G37360.1 (AT4G37360.1 cytochrome P450, family 81, subfamily D, polypeptide 2)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-26
Identity = 62/142 (43.66%), Postives = 92/142 (64.79%), Query Frame = 1 

Query: 16  LLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHG 75
           LL+  S  F KVE+KSMFS+L+FN ++R++AGK YYG+   D+ EA+  R L+ E     
Sbjct: 160 LLRNSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSS 219

Query: 76  GASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRL 135
           G     D++PIL WI +   E  + KL  R D F+Q LV+E+R  K   ++++++++  L
Sbjct: 220 GPGNAADYIPILTWITYS--ETRIKKLAGRLDEFLQGLVDEKREGK---EKKENTMVDHL 279

Query: 136 LELQLSEPEYYTDQIIKGIVLS 158
           L LQ ++PEYY D+IIKG +LS
Sbjct: 280 LCLQETQPEYYMDRIIKGTMLS 296


HSP 2 Score: 95.5 bits (236), Expect = 4.6e-20
Identity = 43/76 (56.58%), Postives = 57/76 (75.00%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           + KL+PFG+GRRACPG  +A R + L+LG LIQC+EW++ G  E+VDMTEG G+T+ K +
Sbjct: 421 AKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE-EEVDMTEGPGLTMPKAR 480

Query: 217 PLETMCKARPIMDKVL 233
           PLE MC+AR  + K+L
Sbjct: 481 PLEAMCRARDFVGKIL 495

[Close]
BLAST of ClCG02G008220 vs. TAIR10
Match: AT1G66540.1 (AT1G66540.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-26
Identity = 59/137 (43.07%), Postives = 87/137 (63.50%), Query Frame = 1 

Query: 21  SESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHGGASQW 80
           S  FAKVE+ SM S L+FN ++R+V GK YYG+   D+ EA+  R+L+ E     GA   
Sbjct: 52  SLEFAKVEMNSMLSNLAFNNIIRMVTGKCYYGDGAEDDPEAKRVRQLIAEAMSCFGAGHA 111

Query: 81  IDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRLLELQL 140
            D +P+L+WI    +E+ + K+  R D F Q LV+E   K+V  ++++++++  LL LQ+
Sbjct: 112 ADHLPMLRWI--TDFERRVKKIAARLDEFFQRLVDE---KRVAKEKKENTMIDHLLSLQV 171

Query: 141 SEPEYYTDQIIKGIVLS 158
           S+PEYYTD  IKG +LS
Sbjct: 172 SQPEYYTDHTIKGTMLS 183


HSP 2 Score: 89.7 bits (221), Expect = 2.6e-18
Identity = 40/75 (53.33%), Postives = 55/75 (73.33%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           ++KL+ FG+GRRACPG  +A R + L+LG LIQC+EW++ G  E+VDMTEGGG+T+ +  
Sbjct: 308 THKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGE-EEVDMTEGGGLTMPRAI 367

Query: 217 PLETMCKARPIMDKV 232
           PL  MC+AR  + K+
Sbjct: 368 PLVAMCRARAFVGKI 381

[Close]
BLAST of ClCG02G008220 vs. NCBI nr
Match: gi|659111597|ref|XP_008455812.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 233.8 bits (595), Expect = 3.1e-58
Identity = 116/142 (81.69%), Postives = 127/142 (89.44%), Query Frame = 1 

Query: 15  LLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRH 74
           LL++LHSESFAKVEL  MFSELSFN VMRVVAGKRYYGE+VSDEAEAREFRELM+E+SR 
Sbjct: 150 LLVKLHSESFAKVELTPMFSELSFNIVMRVVAGKRYYGEKVSDEAEAREFRELMDEVSRQ 209

Query: 75  GGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHR 134
           GG SQW+DFMPILKWIGF  YEK L K    ADRF+QEL+EE RNKKVL +EEQSSLLHR
Sbjct: 210 GGTSQWVDFMPILKWIGFGAYEKILTKSAIWADRFVQELIEEHRNKKVLGREEQSSLLHR 269

Query: 135 LLELQLSEPEYYTDQIIKGIVL 157
           LLELQLS+PEYYTDQIIKG+VL
Sbjct: 270 LLELQLSQPEYYTDQIIKGLVL 291

[Close]
BLAST of ClCG02G008220 vs. NCBI nr
Match: gi|659111597|ref|XP_008455812.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-26
Identity = 64/81 (79.01%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           +NKLIPFGIGRRACPGE M LR +GLTLGLLIQCYEWKK  G E VD TE GGITI KVK
Sbjct: 420 TNKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKV-GYENVDTTEYGGITILKVK 479

Query: 217 PLETMCKARPIMDKVLSNGLD 238
           P+ETMCK  P+M KVLSNGLD
Sbjct: 480 PVETMCKPCPVMVKVLSNGLD 499


HSP 2 Score: 233.4 bits (594), Expect = 4.0e-58
Identity = 115/142 (80.99%), Postives = 129/142 (90.85%), Query Frame = 1 

Query: 15  LLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRH 74
           LL++LHSESFAKVEL  MFSELSFN VMRVVAGKRYYGE+VSD+AEAR+FRELM+E+SR 
Sbjct: 150 LLVKLHSESFAKVELTPMFSELSFNIVMRVVAGKRYYGEKVSDDAEARKFRELMDEVSRQ 209

Query: 75  GGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHR 134
           GG SQW+DFMPI+KWIGF GYEK LAK    ADRF+QELV+E RNKKVL +EEQSSLLHR
Sbjct: 210 GGTSQWVDFMPIMKWIGFGGYEKILAKSAIWADRFVQELVDEHRNKKVLGREEQSSLLHR 269

Query: 135 LLELQLSEPEYYTDQIIKGIVL 157
           LLELQLS+PEYYTDQIIKG+VL
Sbjct: 270 LLELQLSQPEYYTDQIIKGLVL 291

[Close]
BLAST of ClCG02G008220 vs. NCBI nr
Match: gi|449468317|ref|XP_004151868.1| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 126.3 bits (316), Expect = 7.0e-26
Identity = 61/81 (75.31%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           +NKLIPFG+GRRACPGE M LR +GLTLGLLIQCYEWKK  G + VD TE GGITI KVK
Sbjct: 420 TNKLIPFGVGRRACPGEIMGLRVVGLTLGLLIQCYEWKK-HGYDNVDTTEYGGITILKVK 479

Query: 217 PLETMCKARPIMDKVLSNGLD 238
           P+ETMCK RP+M K+LSN LD
Sbjct: 480 PVETMCKPRPVMAKLLSNSLD 499


HSP 2 Score: 233.4 bits (594), Expect = 4.0e-58
Identity = 115/142 (80.99%), Postives = 129/142 (90.85%), Query Frame = 1 

Query: 15  LLLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRH 74
           LL++LHSESFAKVEL  MFSELSFN VMRVVAGKRYYGE+VSD+AEAR+FRELM+E+SR 
Sbjct: 23  LLVKLHSESFAKVELTPMFSELSFNIVMRVVAGKRYYGEKVSDDAEARKFRELMDEVSRQ 82

Query: 75  GGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHR 134
           GG SQW+DFMPI+KWIGF GYEK LAK    ADRF+QELV+E RNKKVL +EEQSSLLHR
Sbjct: 83  GGTSQWVDFMPIMKWIGFGGYEKILAKSAIWADRFVQELVDEHRNKKVLGREEQSSLLHR 142

Query: 135 LLELQLSEPEYYTDQIIKGIVL 157
           LLELQLS+PEYYTDQIIKG+VL
Sbjct: 143 LLELQLSQPEYYTDQIIKGLVL 164

[Close]
BLAST of ClCG02G008220 vs. NCBI nr
Match: gi|700208180|gb|KGN63299.1| (hypothetical protein Csa_2G425760 [Cucumis sativus])

HSP 1 Score: 126.3 bits (316), Expect = 7.0e-26
Identity = 61/81 (75.31%), Postives = 68/81 (83.95%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           +NKLIPFG+GRRACPGE M LR +GLTLGLLIQCYEWKK  G + VD TE GGITI KVK
Sbjct: 293 TNKLIPFGVGRRACPGEIMGLRVVGLTLGLLIQCYEWKK-HGYDNVDTTEYGGITILKVK 352

Query: 217 PLETMCKARPIMDKVLSNGLD 238
           P+ETMCK RP+M K+LSN LD
Sbjct: 353 PVETMCKPRPVMAKLLSNSLD 372


HSP 2 Score: 216.1 bits (549), Expect = 6.7e-53
Identity = 111/145 (76.55%), Postives = 125/145 (86.21%), Query Frame = 1 

Query: 15  LLLQLHSES---FAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEI 74
           LLL+LHS S   F KVELK+MFSEL+FN +MR+VAGKRYYG+EVSDE EAREFR LMEEI
Sbjct: 150 LLLKLHSGSSGEFTKVELKTMFSELAFNALMRIVAGKRYYGDEVSDEEEAREFRGLMEEI 209

Query: 75  SRHGGASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSL 134
           S HGGAS W+DFMPILKWIG  G+EKSL +LTKR D+FMQ L+EERRNKKVL  E ++SL
Sbjct: 210 SLHGGASHWVDFMPILKWIGGGGFEKSLVRLTKRTDKFMQALIEERRNKKVL--ERKNSL 269

Query: 135 LHRLLELQLSEPEYYTDQIIKGIVL 157
           L RLLELQ SEPEYYTDQIIKG+VL
Sbjct: 270 LDRLLELQASEPEYYTDQIIKGLVL 292

[Close]
BLAST of ClCG02G008220 vs. NCBI nr
Match: gi|343466197|gb|AEM42992.1| (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 131.7 bits (330), Expect = 1.7e-27
Identity = 63/81 (77.78%), Postives = 69/81 (85.19%), Query Frame = 1 

Query: 157 SNKLIPFGIGRRACPGETMALRFIGLTLGLLIQCYEWKKAGGCEKVDMTEGGGITIHKVK 216
           S KLI FG+GRR+CPG+TMALRF+GLTLGLLIQCY+WKK G  EKVDM EGGGITIHK K
Sbjct: 421 SKKLIAFGLGRRSCPGDTMALRFVGLTLGLLIQCYQWKKCGD-EKVDMGEGGGITIHKAK 480

Query: 217 PLETMCKARPIMDKVLSNGLD 238
           PLE MCKARP M K+L N LD
Sbjct: 481 PLEAMCKARPAMYKLLLNALD 500


HSP 2 Score: 162.9 bits (411), Expect = 6.7e-37
Identity = 79/141 (56.03%), Postives = 108/141 (76.60%), Query Frame = 1 

Query: 16  LLQLHSESFAKVELKSMFSELSFNTVMRVVAGKRYYGEEVSDEAEAREFRELMEEISRHG 75
           L Q   + F +VE+KSM  EL+FN +MR+VAGKRYYG+EVSDE EAR+FRELM+EI   G
Sbjct: 222 LSQNSVQDFKRVEMKSMLLELTFNIIMRMVAGKRYYGDEVSDEEEARQFRELMKEIFETG 281

Query: 76  GASQWIDFMPILKWIGFDGYEKSLAKLTKRADRFMQELVEERRNKKVLAKEEQSSLLHRL 135
           G S   DF+PIL W+G DG+E+S  +L KR+D+F+Q L++E RN    +KE +++++  L
Sbjct: 282 GVSNPGDFLPILNWLGIDGFERSTKRLAKRSDQFLQGLIDEHRN----SKESRNTMIDHL 341

Query: 136 LELQLSEPEYYTDQIIKGIVL 157
           L LQ S+PEYYTD+IIKG++L
Sbjct: 342 LSLQQSQPEYYTDKIIKGLML 358

The following BLAST results are available for this feature:
BLAST of ClCG02G008220 vs. Swiss-Prot
Match NameE-valueIdentityDescription
C81E8_MEDTR9.4e-3148.25Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1[more]
C81E1_GLYEC9.7e-2843.88Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2[more]
C81E7_MEDTR5.3e-2641.01Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1[more]
C8D11_ARATH1.3e-2441.26Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1[more]
C81D1_ARATH1.7e-2443.08Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1[more]
BLAST of ClCG02G008220 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LQK7_CUCSA2.8e-5880.99Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425760 PE=3 SV=1[more]
A0A0A0LQK7_CUCSA4.9e-2675.31Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425760 PE=3 SV=1[more]
K7NC01_SIRGR1.2e-2777.78Cytochrome P450 OS=Siraitia grosvenorii GN=P450-1 PE=2 SV=1[more]
W9R6T6_9ROSA4.8e-3452.70Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009302 PE=3 SV=1[more]
W9R6T6_9ROSA3.4e-1963.64Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009302 PE=3 SV=1[more]
BLAST of ClCG02G008220 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G37340.17.2e-2948.91 cytochrome P450, family 81, subfamily D, polypeptide 3[more]
AT4G37370.12.7e-2843.80 cytochrome P450, family 81, subfamily D, polypeptide 8[more]
AT2G23220.11.5e-2642.76 cytochrome P450, family 81, subfamily D, polypeptide 6[more]
AT4G37360.11.9e-2643.66 cytochrome P450, family 81, subfamily D, polypeptide 2[more]
AT1G66540.13.3e-2643.07 Cytochrome P450 superfamily protein[more]
BLAST of ClCG02G008220 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659111597|ref|XP_008455812.1|3.1e-5881.69PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo][more]
gi|659111597|ref|XP_008455812.1|1.4e-2679.01PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo][more]
gi|449468317|ref|XP_004151868.1|7.0e-2675.31PREDICTED: cytochrome P450 81E8-like [Cucumis sativus][more]
gi|700208180|gb|KGN63299.1|7.0e-2675.31hypothetical protein Csa_2G425760 [Cucumis sativus][more]
gi|343466197|gb|AEM42992.1|1.7e-2777.78cytochrome P450 [Siraitia grosvenorii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG02G008220.1ClCG02G008220.1mRNA


InterPro
Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 157..225
score: 7.8E-13coord: 18..156
score: 8.8
IPR001128Cytochrome P450PFAMPF00067p450coord: 21..154
score: 1.3E-7coord: 155..214
score: 1.
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 11..225
score: 3.89
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 163..172
scor
NoneNo IPR availableunknownCoilCoilcoord: 92..126
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 15..229
score: 1.9
NoneNo IPR availablePANTHERPTHR24298:SF61CYTOCHROME P450 81D1-RELATEDcoord: 15..229
score: 1.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
ClCG02G008220Lsi10G001710Bottle gourd (USVL1VR-Ls)lsiwcgB055
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
ClCG02G008220Cucurbita maxima (Rimu)cmawcgB484
ClCG02G008220Cucurbita maxima (Rimu)cmawcgB530
ClCG02G008220Cucurbita moschata (Rifu)cmowcgB481
ClCG02G008220Cucurbita moschata (Rifu)cmowcgB528
ClCG02G008220Wild cucumber (PI 183967)cpiwcgB129
ClCG02G008220Cucumber (Chinese Long) v2cuwcgB123
ClCG02G008220Melon (DHL92) v3.5.1mewcgB090
ClCG02G008220Watermelon (97103) v1wcgwmB208
ClCG02G008220Cucurbita pepo (Zucchini)cpewcgB270
ClCG02G008220Cucurbita pepo (Zucchini)cpewcgB663
ClCG02G008220Cucumber (Gy14) v2cgybwcgB120
ClCG02G008220Melon (DHL92) v3.6.1medwcgB088
ClCG02G008220Silver-seed gourdcarwcgB0406
ClCG02G008220Silver-seed gourdcarwcgB0930
ClCG02G008220Cucumber (Chinese Long) v3cucwcgB138
ClCG02G008220Watermelon (97103) v2wcgwmbB138
ClCG02G008220Wax gourdwcgwgoB299
ClCG02G008220Cucumber (Gy14) v1cgywcgB071