Lsi09G008570 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G008570
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	GRAM domain-containing protein / ABA-responsive protein-related
Location	chr09 : 9876587 .. 9878094 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGAAGACAATGGAAGCTATGATGAAACGAGAAGTCACCGGGATCCCGGTTGGCTCCACGACGTTTTGGCCGGTTGAGAAGTCGCTGAAGAGACTGTTACCTGCTCCAGTCGGTAGCCAGTCTCCCAACGGCGAACCCCGGGCAACAAAACAATGTAAAACTTCCATTTTGTTTTTCTTTCCTTCTCTAGATGTCTATATGGTATTTGTAGTGAGGGAGAATGGCAACTGAATGAAGAGTTTTATAAATATTTGCAGGTAGGGAGGATACAGTTGTTAGCAGGAGGAGCATGGTTGGAAGTTTTGCCCAAGGAGTTAGAGAACATGGTAAGCCTTTTCTTCTTGTTTGTTTTTGCTTAATTGGACATTAGTCTCTTAAAAGTGGAGAGAATAACTTGAGTATATACGTAAAAACGTGTCTTGATATGATACGTTTTCTGGGTAGGATTTAAAATAAGACCTAGGTCTAAAGTGCACAATATGATACAATTATGTTAGTGGTAGAGAAGTTTATTGTCATTAACAATGATCGTTTAGAGCATTAGATCGTCTTATTCAAGAAAAGTTATGAAGTGATCTATGAAAATACCTGAGTTTGAATGAGTTTCTTGATCTTAGATGGGCAACTCCTACTTGTTGATGAAGAAGATGAAACTTATAAAGAGACAATATATGATTCTTTAATATATAGAGCCTATAAATAAGACTATTCAACATGTTATTAAACAACTTTATGAGTTCTCTTCAATCAAATTATTATAGTACAGCAGAGAGACCATGAACTTATGTTTATATTGTTTGGTTGGTTAAAGTTGCAGTGAGATTAGGACAAAAGATCAGAGAAACAGTGAAGGGAAAGTTGAATTTGGGAGCAAAGTTTCTTCAAGTTGGTGGGTTGAGGAAAGTTTACAAGCAATTATTTAGTGTAAGAGAAGGAGAGAAACTATTGAAGGCTTGTCAATGCCATTTGTCAACCACAACAGGACCCATAGCAGGTCTTCTCTTCATATCCACACACAAACTTGCCTTTTGCAGTGACAAATCCCTCAAACTCTCTTCCCCAACTGGAGAGCTCCTAAGATTTCATTACAAGGTAACAACCTATCTCTTTCTTAAATCTCTCGAACCGAGCGAAAAGGAACAAGTCCCTTTTCAACTCGTCTTAGATTAAAAAAAGGTTATGATGCTAAAGTGCTCTCTCTGATTTGTCAATTTTTTCAGAATGGGAAGGTTTTATATATATAGATAGTTCGAACGTAAAGTTTTGTCTCTCGTTAAAATGTCGTTCCGCCTCAGTTGTTTACTAAACTGTCTATGATGTTGTTGATGCAGGTGGTGATTCCTGTGGGGAGGATAGAGAGAGTGAACCAAAGTAAGAATATGATGAAGCCTTCACAGAAGTACTTGGAGATAGTAACTGTGGATAATTTTGATTTCTGGTTCATGGGGTTCCAAAATTTCCAAAAAACATTCAGATTTCTTCAACAAGCTATCTCTCAGGAGTGGAAG

mRNA sequence

CAGAAGACAATGGAAGCTATGATGAAACGAGAAGTCACCGGGATCCCGGTTGGCTCCACGACGTTTTGGCCGGTTGAGAAGTCGCTGAAGAGACTGTTACCTGCTCCAGTCGGTAGCCAGTCTCCCAACGGCGAACCCCGGGCAACAAAACAATGTAGGGAGGATACAGTTGTTAGCAGGAGGAGCATGGTTGGAAGTTTTGCCCAAGGAGTTAGAGAACATGTGAGATTAGGACAAAAGATCAGAGAAACAGTGAAGGGAAAGTTGAATTTGGGAGCAAAGTTTCTTCAAGTTGGTGGGTTGAGGAAAGTTTACAAGCAATTATTTAGTGTAAGAGAAGGAGAGAAACTATTGAAGGCTTGTCAATGCCATTTGTCAACCACAACAGGACCCATAGCAGAGAGAGTGAACCAAAGTAAGAATATGATGAAGCCTTCACAGAAGTACTTGGAGATAGTAACTGTGGATAATTTTGATTTCTGGTTCATGGGGTTCCAAAATTTCCAAAAAACATTCAGATTTCTTCAACAAGCTATCTCTCAGGAGTGGAAG

Coding sequence (CDS)

CAGAAGACAATGGAAGCTATGATGAAACGAGAAGTCACCGGGATCCCGGTTGGCTCCACGACGTTTTGGCCGGTTGAGAAGTCGCTGAAGAGACTGTTACCTGCTCCAGTCGGTAGCCAGTCTCCCAACGGCGAACCCCGGGCAACAAAACAATGTAGGGAGGATACAGTTGTTAGCAGGAGGAGCATGGTTGGAAGTTTTGCCCAAGGAGTTAGAGAACATGTGAGATTAGGACAAAAGATCAGAGAAACAGTGAAGGGAAAGTTGAATTTGGGAGCAAAGTTTCTTCAAGTTGGTGGGTTGAGGAAAGTTTACAAGCAATTATTTAGTGTAAGAGAAGGAGAGAAACTATTGAAGGCTTGTCAATGCCATTTGTCAACCACAACAGGACCCATAGCAGAGAGAGTGAACCAAAGTAAGAATATGATGAAGCCTTCACAGAAGTACTTGGAGATAGTAACTGTGGATAATTTTGATTTCTGGTTCATGGGGTTCCAAAATTTCCAAAAAACATTCAGATTTCTTCAACAAGCTATCTCTCAGGAGTGGAAG

Protein sequence

QKTMEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKACQCHLSTTTGPIAERVNQSKNMMKPSQKYLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK

Homology

[Close]

BLAST of Lsi09G008570 vs. Swiss-Prot
Match: GEML8_ARATH (Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.3e-23
Identity = 66/166 (39.76%), Postives = 89/166 (53.61%), Query Frame = 1


Query: 60  RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLK 119
           R+    SF  GVR+  +LG K+ ETVK KL+LGA+ LQ+GGL K+YK+LF V + EKL K
Sbjct: 53  RKKKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFK 112

Query: 120 ACQCHLSTTTGPIAERV-NQSKNMMKPSQKYLEIV------------------------- 179
           A QC+LSTT GPIA  +   SK +   S++ +++                          
Sbjct: 113 AYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQ 172

Query: 180 ---------------TVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                          TVD FDFWFMGF ++QK F  L+QA+S  +K
Sbjct: 173 SQNTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSLSFK 218

[Close]

BLAST of Lsi09G008570 vs. Swiss-Prot
Match: GEML6_ARATH (GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 108.2 bits (269), Expect = 9.5e-23
Identity = 63/164 (38.41%), Postives = 88/164 (53.66%), Query Frame = 1


Query: 60  RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLK 119
           R+    SF  G R+  +LG K+ ETVK KL+LGAK LQ+GGL K+YK+LF V + EKL K
Sbjct: 53  RKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFK 112

Query: 120 ACQCHLSTTTGPIAERV-NQSKNMMKPSQKYLEIV------------------------- 179
           A QC+LSTT GPIA  +   SK +   S++ +++                          
Sbjct: 113 AYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQ 172

Query: 180 ---------------TVDNFDFWFMGFQNFQKTFRFLQQAISQE 183
                          T+DNFDFWFMGF ++QK F  L++A++ +
Sbjct: 173 SQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNND 216

[Close]

BLAST of Lsi09G008570 vs. Swiss-Prot
Match: GEML4_ARATH (GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 67/178 (37.64%), Postives = 93/178 (52.25%), Query Frame = 1


Query: 46  PRATKQCREDTVVS--RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRK 105
           P ++K+  +  V S  +R     F  GVR+  ++  K+ ETVK KL+LGA+ LQVGGL K
Sbjct: 36  PASSKKSEQSNVKSILKRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEK 95

Query: 106 VYKQLFSVREGEKLLKACQCHLSTTTGPIAERV-NQSKNMMKPSQKYLEIV--------- 165
           ++K+LF V EGEKL K  QC+LSTT GPIA  +   SK M   S++ +++          
Sbjct: 96  IFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRV 155

Query: 166 -------------------------------TVDNFDFWFMGFQNFQKTFRFLQQAIS 181
                                          TVD FDFWFMGF ++QK F  L++A+S
Sbjct: 156 HYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of Lsi09G008570 vs. Swiss-Prot
Match: GEML7_ARATH (GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.7e-22
Identity = 63/164 (38.41%), Postives = 86/164 (52.44%), Query Frame = 1


Query: 60  RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLF---------- 119
           ++    SF  G R+  +LG K+ ETVK KL+LGAK LQ+GGL K+YK+LF          
Sbjct: 45  QKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFK 104

Query: 120 ------SVREGE---------KLLKACQCHLSTTTGPIAER----------------VNQ 179
                 S  EG          K +  C       T P  +                 VNQ
Sbjct: 105 AYQCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQ 164

Query: 180 SKNMMKPSQKYLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQE 183
           S+N  KPSQ+YLE+VTVDN+DFWFMGF ++QK F  L++A++++
Sbjct: 165 SQNTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNED 208

[Close]

BLAST of Lsi09G008570 vs. Swiss-Prot
Match: GEML5_ARATH (GEM-like protein 5 OS=Arabidopsis thaliana GN=At5g13200 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 45/154 (29.22%), Postives = 66/154 (42.86%), Query Frame = 1


Query: 68  AQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKACQCHLST 127
           A+ +  +++ G  + ET  GK+NL AK +  GG   +++Q+F     E L K   C+LST
Sbjct: 108 ARNLWHNLKTGPSMSETAWGKVNLTAKAITKGGFESLFRQIFGTEPNETLKKTFACYLST 167

Query: 128 TTGPIAERVNQSKNMM---------------KPSQKYLEIV------------------- 181
           TTGP+A  V  S   +               + S  Y  +V                   
Sbjct: 168 TTGPVAGTVYLSNARVAFCSDRPLYFTAPSGQESWSYYRVVVPLANVATVNPVVVKETPP 227

[Close]

BLAST of Lsi09G008570 vs. TrEMBL
Match: A0A0A0KQ00_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G352640 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.1e-69
Identity = 148/224 (66.07%), Postives = 160/224 (71.43%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           ME MM +EV GIP+ S TF PVEKSLKRLLPAPVG QSP+GEPRATKQCREDTVV  RSM
Sbjct: 1   MEVMMAQEVAGIPISSMTFRPVEKSLKRLLPAPVGCQSPDGEPRATKQCREDTVVGSRSM 60

Query: 64  VGSFAQGVREH--VRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKAC 123
           VGSFAQGVREH  VR+GQKIRETVKGKLNLGAK LQVGGLRK YKQLF VREGEKLLKAC
Sbjct: 61  VGSFAQGVREHVAVRIGQKIRETVKGKLNLGAKILQVGGLRKAYKQLFIVREGEKLLKAC 120

Query: 124 QCHLSTTTGPIAERV-----------NQSKNMMKPSQK---------------------- 183
           QCHLSTT GP+A  +           ++S  +  P+ +                      
Sbjct: 121 QCHLSTTIGPLAGLLFISTHKLAFCSDKSLKLSSPTGELLRFHYKVVIPVGRIERVNQSK 180

Query: 184 --------YLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                   YLEIVTVDNFDFWFMGFQNFQK+FR LQQAISQEWK
Sbjct: 181 NVMKPSQKYLEIVTVDNFDFWFMGFQNFQKSFRSLQQAISQEWK 224

[Close]

BLAST of Lsi09G008570 vs. TrEMBL
Match: M5Y243_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026708mg PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 8.9e-44
Identity = 106/223 (47.53%), Postives = 134/223 (60.09%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           M+A ++ +V GIP+ S  +   E S KRLLP P       G  +     R ++V+ + + 
Sbjct: 1   MKASLQGQVIGIPINSAAY-AFESSPKRLLPDPAIQYPETGPSKLRPTGRGNSVLKKMNK 60

Query: 64  VG----SFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLK 123
           +G    SFA GV+EHVRLG KI ETVKGKL+LGAK +QVGGL KV+K  FSVR GEKLLK
Sbjct: 61  LGKKADSFAHGVKEHVRLGPKITETVKGKLSLGAKIIQVGGLEKVFKHAFSVRAGEKLLK 120

Query: 124 ACQCHLSTTTGPIA-----------------------------------------ERVNQ 182
             QC+LSTT GPIA                                         +RVNQ
Sbjct: 121 VSQCYLSTTAGPIAGLLFISTDKIAFCSERSIKLSSPNGELIRVHYKVVIPVNKIKRVNQ 180

[Close]

BLAST of Lsi09G008570 vs. TrEMBL
Match: A0A0D2VA77_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G067700 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.2e-43
Identity = 108/226 (47.79%), Postives = 137/226 (60.62%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCRE---DTVVSR 63
           M+  +  +V G+P+ ST +  VE++ +R LP   G          +   RE   + V+ R
Sbjct: 1   MKNQILEQVIGVPMKSTLY-RVERTPRRYLPDSAGQYRIPSSVEGSNTFREGKRNFVLKR 60

Query: 64  RSMVG----SFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEK 123
            + +G    +FA GVREHVRLG KI ETVKGKL+LGA+ LQVGG+ K++KQLFSVREGEK
Sbjct: 61  INNIGKKADTFAHGVREHVRLGPKISETVKGKLSLGARILQVGGVEKIFKQLFSVREGEK 120

Query: 124 LLKACQCHLSTTTGPIA-----------------------------------------ER 182
           LLKACQCHLSTT GPIA                                         + 
Sbjct: 121 LLKACQCHLSTTAGPIAGLLFISSQKVAFCSDRSIKVPSPNGEYLRVHYKVLIPLEKIKG 180

[Close]

BLAST of Lsi09G008570 vs. TrEMBL
Match: A0A0B0PSS4_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_33548 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.9e-43
Identity = 106/223 (47.53%), Postives = 134/223 (60.09%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           M+  M ++VTG+P+ + T+    ++ +  LP   G    + +  ATK  + + V+ R ++
Sbjct: 1   MKNQMLKQVTGVPIIAATYGVERRTSRHYLPDAAGQYHISSQG-ATKS-KGNFVLERMNL 60

Query: 64  VG----SFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLK 123
           +G    +FA GVREHVRLG KI ETVKGKL+LGA+ LQVGGL  V+KQLFS REGEKLLK
Sbjct: 61  IGKKAGTFAHGVREHVRLGPKISETVKGKLSLGARILQVGGLENVFKQLFSFREGEKLLK 120

Query: 124 ACQCHLSTTTGPIA-----------------------------------------ERVNQ 182
           ACQC+LSTT GPIA                                         +RVNQ
Sbjct: 121 ACQCYLSTTAGPIAGLLFISSEKVAFCSERSIKIPCPNGEYVRVHYKVVVPVEKIKRVNQ 180

[Close]

BLAST of Lsi09G008570 vs. TrEMBL
Match: A0A067L7J7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24479 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.7e-42
Identity = 107/228 (46.93%), Postives = 137/228 (60.09%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLL---PAPVGSQSPNGEPRATKQCREDTVVSR 63
           M+   + +V GIP+ S     VE+  + LL    AP    +P  +  A KQ + D+V+ R
Sbjct: 1   MKNSTQEQVIGIPITSAAN-AVERMPRILLLENAAPTYVPTPANKSLAVKQDKVDSVLKR 60

Query: 64  RSMVGS----FAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEK 123
            + +G     FA G+REHVRLG KI ET+KGKL+LGA+ LQVGG++KV++QLF+VREGEK
Sbjct: 61  MNKLGKKADKFAHGIREHVRLGAKITETLKGKLSLGARILQVGGVKKVFRQLFTVREGEK 120

Query: 124 LLKACQCHLSTTTGPIAERV---------------------------------------- 183
           LLKACQC+LSTT GPIA  V                                        
Sbjct: 121 LLKACQCYLSTTAGPIAGLVFISTDKIAFCSERSIKLSSTEGKIARIHYKVVIPLRKIKT 180

[Close]

BLAST of Lsi09G008570 vs. TAIR10
Match: AT5G23370.1 (AT5G23370.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 109.8 bits (273), Expect = 1.8e-24
Identity = 66/166 (39.76%), Postives = 89/166 (53.61%), Query Frame = 1


Query: 60  RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLK 119
           R+    SF  GVR+  +LG K+ ETVK KL+LGA+ LQ+GGL K+YK+LF V + EKL K
Sbjct: 53  RKKKNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFK 112

Query: 120 ACQCHLSTTTGPIAERV-NQSKNMMKPSQKYLEIV------------------------- 179
           A QC+LSTT GPIA  +   SK +   S++ +++                          
Sbjct: 113 AYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQ 172

Query: 180 ---------------TVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                          TVD FDFWFMGF ++QK F  L+QA+S  +K
Sbjct: 173 SQNTTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEQALSLSFK 218

[Close]

BLAST of Lsi09G008570 vs. TAIR10
Match: AT5G23350.1 (AT5G23350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 108.2 bits (269), Expect = 5.4e-24
Identity = 63/164 (38.41%), Postives = 88/164 (53.66%), Query Frame = 1


Query: 60  RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLK 119
           R+    SF  G R+  +LG K+ ETVK KL+LGAK LQ+GGL K+YK+LF V + EKL K
Sbjct: 115 RKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFK 174

Query: 120 ACQCHLSTTTGPIAERV-NQSKNMMKPSQKYLEIV------------------------- 179
           A QC+LSTT GPIA  +   SK +   S++ +++                          
Sbjct: 175 AYQCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQ 234

Query: 180 ---------------TVDNFDFWFMGFQNFQKTFRFLQQAISQE 183
                          T+DNFDFWFMGF ++QK F  L++A++ +
Sbjct: 235 SQNTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLEKALNND 278

[Close]

BLAST of Lsi09G008570 vs. TAIR10
Match: AT5G08350.1 (AT5G08350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 107.8 bits (268), Expect = 7.0e-24
Identity = 67/178 (37.64%), Postives = 93/178 (52.25%), Query Frame = 1


Query: 46  PRATKQCREDTVVS--RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRK 105
           P ++K+  +  V S  +R     F  GVR+  ++  K+ ETVK KL+LGA+ LQVGGL K
Sbjct: 36  PASSKKSEQSNVKSILKRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEK 95

Query: 106 VYKQLFSVREGEKLLKACQCHLSTTTGPIAERV-NQSKNMMKPSQKYLEIV--------- 165
           ++K+LF V EGEKL K  QC+LSTT GPIA  +   SK M   S++ +++          
Sbjct: 96  IFKRLFRVSEGEKLFKMYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRV 155

Query: 166 -------------------------------TVDNFDFWFMGFQNFQKTFRFLQQAIS 181
                                          TVD FDFWFMGF ++QK F  L++A+S
Sbjct: 156 HYKVSIPLCKIDRVNQSQNTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLEKALS 213

[Close]

BLAST of Lsi09G008570 vs. TAIR10
Match: AT5G23360.1 (AT5G23360.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 105.9 bits (263), Expect = 2.7e-23
Identity = 63/164 (38.41%), Postives = 86/164 (52.44%), Query Frame = 1


Query: 60  RRSMVGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLF---------- 119
           ++    SF  G R+  +LG K+ ETVK KL+LGAK LQ+GGL K+YK+LF          
Sbjct: 45  QKKKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFK 104

Query: 120 ------SVREGE---------KLLKACQCHLSTTTGPIAER----------------VNQ 179
                 S  EG          K +  C       T P  +                 VNQ
Sbjct: 105 AYQCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQ 164

Query: 180 SKNMMKPSQKYLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQE 183
           S+N  KPSQ+YLE+VTVDN+DFWFMGF ++QK F  L++A++++
Sbjct: 165 SQNTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLEKALNED 208

[Close]

BLAST of Lsi09G008570 vs. TAIR10
Match: AT4G40100.1 (AT4G40100.1 GRAM domain family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-12
Identity = 43/176 (24.43%), Postives = 80/176 (45.45%), Query Frame = 1


Query: 24  PVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSMVGSFAQGVREHVRLGQKIR- 83
           P EK+L     A             +    ++T+ S + ++G + + V E     + +  
Sbjct: 41  PDEKALSSSSAARSNPYVARAPTETSDASLKETMESVKGVLGRWGRRVGEAAMKAESLAG 100

Query: 84  -------ETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKACQCHLSTTTGPIA-- 143
                      G++    K L  GG  K+++Q F     E+L  +  C+LST+ GP+   
Sbjct: 101 NTWQHPLRAAMGRIAQSTKVLAEGGYEKIFRQTFETVPEEQLQNSFACYLSTSAGPVMGV 160

Query: 144 ----------ERVNQSKNMMKPSQKYLEIVTVDNFDFWFMGFQNFQKTFRFLQQAI 180
                     + VN S + + P++KY+++++VD+ +FWFMGF N++     LQ  +
Sbjct: 161 LYVVIPLHQLKSVNPSISTVNPAEKYIQVISVDDHEFWFMGFLNYEGAVTSLQDTL 216

[Close]

BLAST of Lsi09G008570 vs. NCBI nr
Match: gi|659072316|ref|XP_008465120.1| (PREDICTED: GEM-like protein 4 isoform X2 [Cucumis melo])

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-72
Identity = 154/222 (69.37%), Postives = 163/222 (73.42%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           ME  M REV  IPVGSTT  PVEKSLKRLLPAPVG QSP+ EPRATKQCREDTVVSRRSM
Sbjct: 1   MEVRMAREVARIPVGSTTLRPVEKSLKRLLPAPVGCQSPDVEPRATKQCREDTVVSRRSM 60

Query: 64  VGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKACQC 123
           VGSFAQGVREHVRLGQKIRETVKGKLNLGAK LQVGGLRKVYKQLFSVREGEKLLKACQC
Sbjct: 61  VGSFAQGVREHVRLGQKIRETVKGKLNLGAKILQVGGLRKVYKQLFSVREGEKLLKACQC 120

Query: 124 HLSTTTGPIAERV-----------NQSKNMMKPSQK------------------------ 183
           HLSTTTGP+A  +           ++S  +  P+ +                        
Sbjct: 121 HLSTTTGPLAGLLFISTHKLAFCSDKSLKLSSPTGELLRFHYKVVIPVGRIERVNQSKNV 180

Query: 184 ------YLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                 YLEIVTVDNFDFWFMGFQNFQK+FR LQQAISQEWK
Sbjct: 181 MKPSQKYLEIVTVDNFDFWFMGFQNFQKSFRSLQQAISQEWK 222

[Close]

BLAST of Lsi09G008570 vs. NCBI nr
Match: gi|659072314|ref|XP_008465112.1| (PREDICTED: GEM-like protein 4 isoform X1 [Cucumis melo])

HSP 1 Score: 276.2 bits (705), Expect = 4.2e-71
Identity = 154/224 (68.75%), Postives = 163/224 (72.77%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           ME  M REV  IPVGSTT  PVEKSLKRLLPAPVG QSP+ EPRATKQCREDTVVSRRSM
Sbjct: 1   MEVRMAREVARIPVGSTTLRPVEKSLKRLLPAPVGCQSPDVEPRATKQCREDTVVSRRSM 60

Query: 64  VGSFAQGVREH--VRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKAC 123
           VGSFAQGVREH  VRLGQKIRETVKGKLNLGAK LQVGGLRKVYKQLFSVREGEKLLKAC
Sbjct: 61  VGSFAQGVREHVAVRLGQKIRETVKGKLNLGAKILQVGGLRKVYKQLFSVREGEKLLKAC 120

Query: 124 QCHLSTTTGPIAERV-----------NQSKNMMKPSQK---------------------- 183
           QCHLSTTTGP+A  +           ++S  +  P+ +                      
Sbjct: 121 QCHLSTTTGPLAGLLFISTHKLAFCSDKSLKLSSPTGELLRFHYKVVIPVGRIERVNQSK 180

Query: 184 --------YLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                   YLEIVTVDNFDFWFMGFQNFQK+FR LQQAISQEWK
Sbjct: 181 NVMKPSQKYLEIVTVDNFDFWFMGFQNFQKSFRSLQQAISQEWK 224

[Close]

BLAST of Lsi09G008570 vs. NCBI nr
Match: gi|449464300|ref|XP_004149867.1| (PREDICTED: GEM-like protein 4 isoform X2 [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 9.4e-71
Identity = 148/222 (66.67%), Postives = 160/222 (72.07%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           ME MM +EV GIP+ S TF PVEKSLKRLLPAPVG QSP+GEPRATKQCREDTVV  RSM
Sbjct: 1   MEVMMAQEVAGIPISSMTFRPVEKSLKRLLPAPVGCQSPDGEPRATKQCREDTVVGSRSM 60

Query: 64  VGSFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKACQC 123
           VGSFAQGVREHVR+GQKIRETVKGKLNLGAK LQVGGLRK YKQLF VREGEKLLKACQC
Sbjct: 61  VGSFAQGVREHVRIGQKIRETVKGKLNLGAKILQVGGLRKAYKQLFIVREGEKLLKACQC 120

Query: 124 HLSTTTGPIAERV-----------NQSKNMMKPSQK------------------------ 183
           HLSTT GP+A  +           ++S  +  P+ +                        
Sbjct: 121 HLSTTIGPLAGLLFISTHKLAFCSDKSLKLSSPTGELLRFHYKVVIPVGRIERVNQSKNV 180

Query: 184 ------YLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                 YLEIVTVDNFDFWFMGFQNFQK+FR LQQAISQEWK
Sbjct: 181 MKPSQKYLEIVTVDNFDFWFMGFQNFQKSFRSLQQAISQEWK 222

[Close]

BLAST of Lsi09G008570 vs. NCBI nr
Match: gi|778702228|ref|XP_011655160.1| (PREDICTED: GEM-like protein 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 270.0 bits (689), Expect = 3.0e-69
Identity = 148/224 (66.07%), Postives = 160/224 (71.43%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQSPNGEPRATKQCREDTVVSRRSM 63
           ME MM +EV GIP+ S TF PVEKSLKRLLPAPVG QSP+GEPRATKQCREDTVV  RSM
Sbjct: 1   MEVMMAQEVAGIPISSMTFRPVEKSLKRLLPAPVGCQSPDGEPRATKQCREDTVVGSRSM 60

Query: 64  VGSFAQGVREH--VRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEKLLKAC 123
           VGSFAQGVREH  VR+GQKIRETVKGKLNLGAK LQVGGLRK YKQLF VREGEKLLKAC
Sbjct: 61  VGSFAQGVREHVAVRIGQKIRETVKGKLNLGAKILQVGGLRKAYKQLFIVREGEKLLKAC 120

Query: 124 QCHLSTTTGPIAERV-----------NQSKNMMKPSQK---------------------- 183
           QCHLSTT GP+A  +           ++S  +  P+ +                      
Sbjct: 121 QCHLSTTIGPLAGLLFISTHKLAFCSDKSLKLSSPTGELLRFHYKVVIPVGRIERVNQSK 180

Query: 184 --------YLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
                   YLEIVTVDNFDFWFMGFQNFQK+FR LQQAISQEWK
Sbjct: 181 NVMKPSQKYLEIVTVDNFDFWFMGFQNFQKSFRSLQQAISQEWK 224

[Close]

BLAST of Lsi09G008570 vs. NCBI nr
Match: gi|659072318|ref|XP_008465126.1| (PREDICTED: putative GEM-like protein 8 [Cucumis melo])

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-48
Identity = 115/229 (50.22%), Postives = 147/229 (64.19%), Query Frame = 1


Query: 4   MEAMMKREVTGIPVGSTTFWPVEKSLKRLLPAPVGSQS-PNGEPR--ATKQCREDTVVSR 63
           ME +++R V G P+GST    VE+S++RLLP   G  + P+ + +    KQC +D+++SR
Sbjct: 1   METLLQRHVIGFPIGSTAL-QVEQSVQRLLPDLAGQYNIPSSDTKYPTLKQCGKDSMLSR 60

Query: 64  RSMVG----SFAQGVREHVRLGQKIRETVKGKLNLGAKFLQVGGLRKVYKQLFSVREGEK 123
            + +G    SFA GVREHVRLG KI ETVKGKL+LGA+ LQVGGLRK++KQLF+V +GEK
Sbjct: 61  VNKLGKRADSFAHGVREHVRLGSKISETVKGKLSLGARILQVGGLRKIFKQLFNVGDGEK 120

Query: 124 LLKACQCHLSTTTGPIA-----------------------------------------ER 183
           LLKA  C+LSTT GPIA                                         ER
Sbjct: 121 LLKAVHCYLSTTAGPIAGLLFISTDKIAFCSDRSIKVSSPSGELIRFHYKVVIPVGKIER 180

Query: 184 VNQSKNMMKPSQKYLEIVTVDNFDFWFMGFQNFQKTFRFLQQAISQEWK 185
           +NQS+N+ KPSQKY+EIVTVDNFDFWFMGF N+ KT ++LQ+ ISQE K
Sbjct: 181 INQSENVKKPSQKYMEIVTVDNFDFWFMGFLNYHKTLQYLQEVISQECK 228

The following BLAST results are available for this feature:

BLAST of Lsi09G008570 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GEML8_ARATH	3.3e-23	39.76	Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1	[more]
GEML6_ARATH	9.5e-23	38.41	GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2	[more]
GEML4_ARATH	1.2e-22	37.64	GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1	[more]
GEML7_ARATH	4.7e-22	38.41	GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1	[more]
GEML5_ARATH	1.4e-10	29.22	GEM-like protein 5 OS=Arabidopsis thaliana GN=At5g13200 PE=1 SV=1	[more]

BLAST of Lsi09G008570 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQ00_CUCSA	2.1e-69	66.07	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G352640 PE=4 SV=1	[more]
M5Y243_PRUPE	8.9e-44	47.53	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026708mg PE=4 SV=1	[more]
A0A0D2VA77_GOSRA	1.2e-43	47.79	Uncharacterized protein OS=Gossypium raimondii GN=B456_013G067700 PE=4 SV=1	[more]
A0A0B0PSS4_GOSAR	9.9e-43	47.53	Uncharacterized protein OS=Gossypium arboreum GN=F383_33548 PE=4 SV=1	[more]
A0A067L7J7_JATCU	1.7e-42	46.93	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24479 PE=4 SV=1	[more]

BLAST of Lsi09G008570 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23370.1	1.8e-24	39.76	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23350.1	5.4e-24	38.41	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G08350.1	7.0e-24	37.64	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT5G23360.1	2.7e-23	38.41	GRAM domain-containing protein / ABA-responsive protein-related	[more]
AT4G40100.1	1.2e-12	24.43	GRAM domain family protein	[more]

BLAST of Lsi09G008570 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659072316\|ref\|XP_008465120.1\|	1.3e-72	69.37	PREDICTED: GEM-like protein 4 isoform X2 [Cucumis melo]	[more]
gi\|659072314\|ref\|XP_008465112.1\|	4.2e-71	68.75	PREDICTED: GEM-like protein 4 isoform X1 [Cucumis melo]	[more]
gi\|449464300\|ref\|XP_004149867.1\|	9.4e-71	66.67	PREDICTED: GEM-like protein 4 isoform X2 [Cucumis sativus]	[more]
gi\|778702228\|ref\|XP_011655160.1\|	3.0e-69	66.07	PREDICTED: GEM-like protein 4 isoform X1 [Cucumis sativus]	[more]
gi\|659072318\|ref\|XP_008465126.1\|	1.0e-48	50.22	PREDICTED: putative GEM-like protein 8 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G008570.1	Lsi09G008570.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31969	FAMILY NOT NAMED	coord: 66..181 score: 4.6
None	No IPR available	PANTHER	PTHR31969:SF1	GEM-LIKE PROTEIN 4-RELATED	coord: 66..181 score: 4.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G008570	Carg01411	Silver-seed gourd	carlsiB754

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G008570	Cucurbita moschata (Rifu)	cmolsiB610
Lsi09G008570	Cucurbita pepo (Zucchini)	cpelsiB130
Lsi09G008570	Cucurbita pepo (Zucchini)	cpelsiB311
Lsi09G008570	Cucurbita pepo (Zucchini)	cpelsiB317
Lsi09G008570	Wild cucumber (PI 183967)	cpilsiB331
Lsi09G008570	Cucumber (Chinese Long) v2	culsiB331
Lsi09G008570	Melon (DHL92) v3.5.1	lsimeB013
Lsi09G008570	Melon (DHL92) v3.5.1	lsimeB015
Lsi09G008570	Watermelon (Charleston Gray)	lsiwcgB008
Lsi09G008570	Watermelon (Charleston Gray)	lsiwcgB012
Lsi09G008570	Watermelon (97103) v1	lsiwmB029
Lsi09G008570	Watermelon (97103) v1	lsiwmB034
Lsi09G008570	Cucumber (Gy14) v2	cgyblsiB303
Lsi09G008570	Melon (DHL92) v3.6.1	lsimedB014
Lsi09G008570	Melon (DHL92) v3.6.1	lsimedB016
Lsi09G008570	Silver-seed gourd	carlsiB433
Lsi09G008570	Cucumber (Chinese Long) v3	cuclsiB354
Lsi09G008570	Watermelon (97103) v2	lsiwmbB007
Lsi09G008570	Watermelon (97103) v2	lsiwmbB009
Lsi09G008570	Watermelon (97103) v2	lsiwmbB012
Lsi09G008570	Wax gourd	lsiwgoB009
Lsi09G008570	Bottle gourd (USVL1VR-Ls)	lsilsiB004
Lsi09G008570	Cucumber (Gy14) v1	cgylsiB350
Lsi09G008570	Cucurbita maxima (Rimu)	cmalsiB268
Lsi09G008570	Cucurbita maxima (Rimu)	cmalsiB622
Lsi09G008570	Cucurbita maxima (Rimu)	cmalsiB629
Lsi09G008570	Cucurbita moschata (Rifu)	cmolsiB256