Lsi09G001730 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi09G001730
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionTrehalase
Locationchr09 : 1814876 .. 1817908 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCATTACTCCGTCCCGCCGCCATGCCGTTGCCGGAAGTTTCTTCCCCACGGGTCGCCGATAGGGGTCCTGTGGTTCCGGTCACCAATCTCGTCAAGTTCCTTGAGCGTCTTCAGGTTGTAGCCCTCAACTTGTTTGGGAAATTGGATTTCGATCTTAAATACTATGTCGATTTGTCTCTGAAATTCGATTTGAATTCTACCCAAACCGCTTTCGATGCCCTTGAACGATCCTCCAATGACTCTGTTCCCGTTGAGAATTTGAAGGCCTTCATTACAGACTATTTCGATAGTGCTGGAACCGACTTGGTTTACTCTGAATCGGTTGATTTTGTTCCTCGGCCTGTTGGGTTTTTGCCCAAAGTTGAGAATGCGGAGGTTAGAGCTTGGGGTTTGGATATTCATAACTTCTGCAAGAATCTTAGCCGTAGAGTCTCTGATGATCTCATTCATCGACCGGATACTCATACTCTGTTGCCGTTGCCGGAGCCTGTTGTCGTTCCGGGATCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGGTATGCGGGCGAAATTTAGTTCTCGAGTTCTTTTTTTATGAATCTTTTACTCTGCAACAGTTTCACTTGATGACTCGTTCTCCACATCGTTCTTAACCCTTTTCTTTGGCTCTTTCACCCCTTTTGTCATTTAGTGAAGAAACAATTCTCAGCTTGAAATTCATTATTAAGAAAGGGGAAATCTGATGGTAGAATGAAAATAATATATTATTATTTTCTTAGAGTTATGACAAACTAAGTGGGGTTGTTTCCTAATTTGTTTTTCTTGGCACTGTTCTGCTTGATAATGCCTTTTCTTTTTATATTTATGTGCAGAGGTTTGTTAGTTAGTAAAATGTATGATACTGCAAAGGGAATTGTTATAAATCTCATTTCAATGATTGATGAGTTTGGCCATGTTCTGAATGGTGCCAGAGCTTATTACACAAACAGAAGGTAAACAAATCATCTTTTGAATTGAACTGCTTTAAAATAACTTGATCATTAATGCAGTTTGAACTCATTATCAAACTTTTTATTGCATTCTGCAGTCAGCCTCCCCTTTTGAGTTCCATGGTTTATGACATATACCTTAGGACAGGGGATTTAGAGTTTGTGAGCAATGCACTCCCAGCCTTAATTAAAGAGCATAAGTTTTGGAATTCAGGTATGACAAGACATCGATGTTTCTTACTCAATCAGATATTTGGTTTTAGTAAGTTTGTAATGTAGTCTTTTTTTTCTGTTTGTTTTAGGATTCCATTCAATTACTGTCCGGAGTGCTCCTGGTGGAAATGAAAATCACTCTTTATCTAGATACTATGCAATGTGGAACGAGCCGAGGCCTGAATCCTCATTGCTGGTGTGTTTTCCTATTTAATCTCAGTTACCTGCATTTGAAATAATAATATGCAACCTTGTGTTTGATTCTATGATTTACTATTGGCAGGATGAGAAACTTGCCTCAAAGTTTGTTAATAACTATGAAAAAAAGGCATCTATACCGGGAAATCGCATCGGCAGCCAAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGTACAACATTATGTGATCTCAGATCTTAAATATCTGCCGAGCTGAGAGTTCGATTCATTTTTCAAGTATTACAACAATGAAGTTTTTAATGCTTTTGAGTTTGCTTCTTAAAGGCATTCGACAGACTTATCAACATTGGCTACAACTTCAATCCTGCCAGTTGATCTCAATGTATTCATACTCAAGGTATGGCTTGGATGAGTTATTCGAAAATCTGGACATTTTTCTTTTTATTGATGTGTATGCTCTGATTCAGATGGAACTTGACATTTCCAATTTGGCAAGAGCTGTTGGAGATTACTGCACGGCAGAACACTTTTTTGAAGCTTCTATAGTTAGAAAGAAGACAATCAACTCTATTTTTTGGAATTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCATACAAGGTAAATGTTCATCTTTTGGATGTTTTCTTTTAGTAGTTATGTTGATTGAAACTTTTGGTGCTTTCAGGGTGCTCATACATGGGATGCTCGAAACCAGAACCAGAACGTATACGCTTCAAACTTCATTCTGTTGTGGATCGAATCGTTCTACTCTGGTTTGTAGCTAATCCTCTCATGATCATAAGGATTTTTTTTATCCAAATTTCTCTAGAAGTTAAGATTAAGAATGCTAAGATTAATTCAAATATTCTGGTTTTCTATAAGCAGATAACAAACGGATGGTGAAAGTCTTGAAAAGTTTACGGAACTCAGGCTTGTTGTGCAATGCTGGGATTGCAACTTCAATGATTAATTCTGGAGAACAATGGTTAGTAGTACTAACTCTTAGGAACTGGTCTTCTTTTTTCTTTTGAATGTCTTTGTTTGATATTCATACTGAAACCTTCTGTAAAACAAAAGGATTTCCCGAATGGTTGGGCGCCAATCCAGCACATGATCGTCGAGGGACTGGCAAGGTCCGGAATGAAAGAAGCAAGGGCATTGGCCGAGGACATTGCTACAAGATGGCTTCGAACCAACTATGTAGCTTACAAGCACACAGGATATATTTATGAGAAATATGATGTCCAAAAGTGTGGAGACTTTGGTGGAAGAGGTGAATATTCCCTCAGGTATATGAATCAATTACCTTTGCTTCTTCTTTTTCGGCTATGTTTGATAACTATTAAGTTTTTGAATTTTGATTTTTGAAAATTAAGCTTATAAACACTACTTCCACTTGTATGAAAACTGTAATATAGAATTAACAAGCCAGCTTCAAAAACAGAAAACTAAAAGCAAAATTGTTATTAAACAAGATGTAATTCAACCATCATTTTTCCTTTTTTTAATTGATTGCTTATCTGTTTCTGCTTGGTGTTTTTGTTTGAATGTAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGACATTCCTTGAAGAGTTTGGATGGCCTGAAGATCAAGGGATAGATTGCTGA

mRNA sequence

ATGCCATTACTCCGTCCCGCCGCCATGCCGTTGCCGGAAGTTTCTTCCCCACGGGTCGCCGATAGGGGTCCTGTGGTTCCGGTCACCAATCTCGTCAAGTTCCTTGAGCGTCTTCAGGTTGTAGCCCTCAACTTGTTTGGGAAATTGGATTTCGATCTTAAATACTATGTCGATTTGTCTCTGAAATTCGATTTGAATTCTACCCAAACCGCTTTCGATGCCCTTGAACGATCCTCCAATGACTCTGTTCCCGTTGAGAATTTGAAGGCCTTCATTACAGACTATTTCGATAGTGCTGGAACCGACTTGGTTTACTCTGAATCGGTTGATTTTGTTCCTCGGCCTGTTGGGTTTTTGCCCAAAGTTGAGAATGCGGAGGTTAGAGCTTGGGGTTTGGATATTCATAACTTCTGCAAGAATCTTAGCCGTAGAGTCTCTGATGATCTCATTCATCGACCGGATACTCATACTCTGTTGCCGTTGCCGGAGCCTGTTGTCGTTCCGGGATCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGAGGTTTGTTAGTTAGTAAAATGTATGATACTGCAAAGGGAATTGTTATAAATCTCATTTCAATGATTGATGAGTTTGGCCATGTTCTGAATGGTGCCAGAGCTTATTACACAAACAGAAGTCAGCCTCCCCTTTTGAGTTCCATGGTTTATGACATATACCTTAGGACAGGGGATTTAGAGTTTGTGAGCAATGCACTCCCAGCCTTAATTAAAGAGCATAAGTTTTGGAATTCAGGATTCCATTCAATTACTGTCCGGAGTGCTCCTGGTGGAAATGAAAATCACTCTTTATCTAGATACTATGCAATGTGGAACGAGCCGAGGCCTGAATCCTCATTGCTGGATGAGAAACTTGCCTCAAAGTTTGTTAATAACTATGAAAAAAAGGCATCTATACCGGGAAATCGCATCGGCAGCCAAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGTACAACATTATATGGAACTTGACATTTCCAATTTGGCAAGAGCTGTTGGAGATTACTGCACGGCAGAACACTTTTTTGAAGCTTCTATAGTTAGAAAGAAGACAATCAACTCTATTTTTTGGAATTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCATACAAGGGTGCTCATACATGGGATGCTCGAAACCAGAACCAGAACGTATACGCTTCAAACTTCATTCTGTTGTGGATCGAATCGTTCTACTCTGATAACAAACGGATGGTGAAAGTCTTGAAAAGTTTACGGAACTCAGGCTTGTTGTGCAATGCTGGGATTGCAACTTCAATGATTAATTCTGGAGAACAATGGTTAGTAGATTTCCCGAATGGTTGGGCGCCAATCCAGCACATGATCGTCGAGGGACTGGCAAGGTCCGGAATGAAAGAAGCAAGGGCATTGGCCGAGGACATTGCTACAAGATGGCTTCGAACCAACTATACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGACATTCCTTGAAGAGTTTGGATGGCCTGAAGATCAAGGGATAGATTGCTGA

Coding sequence (CDS)

ATGCCATTACTCCGTCCCGCCGCCATGCCGTTGCCGGAAGTTTCTTCCCCACGGGTCGCCGATAGGGGTCCTGTGGTTCCGGTCACCAATCTCGTCAAGTTCCTTGAGCGTCTTCAGGTTGTAGCCCTCAACTTGTTTGGGAAATTGGATTTCGATCTTAAATACTATGTCGATTTGTCTCTGAAATTCGATTTGAATTCTACCCAAACCGCTTTCGATGCCCTTGAACGATCCTCCAATGACTCTGTTCCCGTTGAGAATTTGAAGGCCTTCATTACAGACTATTTCGATAGTGCTGGAACCGACTTGGTTTACTCTGAATCGGTTGATTTTGTTCCTCGGCCTGTTGGGTTTTTGCCCAAAGTTGAGAATGCGGAGGTTAGAGCTTGGGGTTTGGATATTCATAACTTCTGCAAGAATCTTAGCCGTAGAGTCTCTGATGATCTCATTCATCGACCGGATACTCATACTCTGTTGCCGTTGCCGGAGCCTGTTGTCGTTCCGGGATCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGAGGTTTGTTAGTTAGTAAAATGTATGATACTGCAAAGGGAATTGTTATAAATCTCATTTCAATGATTGATGAGTTTGGCCATGTTCTGAATGGTGCCAGAGCTTATTACACAAACAGAAGTCAGCCTCCCCTTTTGAGTTCCATGGTTTATGACATATACCTTAGGACAGGGGATTTAGAGTTTGTGAGCAATGCACTCCCAGCCTTAATTAAAGAGCATAAGTTTTGGAATTCAGGATTCCATTCAATTACTGTCCGGAGTGCTCCTGGTGGAAATGAAAATCACTCTTTATCTAGATACTATGCAATGTGGAACGAGCCGAGGCCTGAATCCTCATTGCTGGATGAGAAACTTGCCTCAAAGTTTGTTAATAACTATGAAAAAAAGGCATCTATACCGGGAAATCGCATCGGCAGCCAAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGTACAACATTATATGGAACTTGACATTTCCAATTTGGCAAGAGCTGTTGGAGATTACTGCACGGCAGAACACTTTTTTGAAGCTTCTATAGTTAGAAAGAAGACAATCAACTCTATTTTTTGGAATTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCATACAAGGGTGCTCATACATGGGATGCTCGAAACCAGAACCAGAACGTATACGCTTCAAACTTCATTCTGTTGTGGATCGAATCGTTCTACTCTGATAACAAACGGATGGTGAAAGTCTTGAAAAGTTTACGGAACTCAGGCTTGTTGTGCAATGCTGGGATTGCAACTTCAATGATTAATTCTGGAGAACAATGGTTAGTAGATTTCCCGAATGGTTGGGCGCCAATCCAGCACATGATCGTCGAGGGACTGGCAAGGTCCGGAATGAAAGAAGCAAGGGCATTGGCCGAGGACATTGCTACAAGATGGCTTCGAACCAACTATACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGACATTCCTTGAAGAGTTTGGATGGCCTGAAGATCAAGGGATAGATTGCTGA

Protein sequence

MPLLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKKASIPGNRIGSQIWLGFQFKMDEVQHYMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNYTGFGWSNGVVLTFLEEFGWPEDQGIDC
BLAST of Lsi09G001730 vs. Swiss-Prot
Match: TRE1_ARATH (Trehalase OS=Arabidopsis thaliana GN=TRE1 PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 2.6e-150
Identity = 277/561 (49.38%), Postives = 359/561 (63.99%), Query Frame = 1

Query: 21  DRGPVVPVTNLVKFLERLQVVALNLFGKLDF-DLKYYVDLSLK--FDLNSTQTAFDALER 80
           D GPVV  T LV FL+R+Q  AL  + K    D K Y+DLSLK  + L++ ++AFD L  
Sbjct: 70  DSGPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTS 129

Query: 81  SSNDS-VPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVRAWGLDIHN 140
            S+D  VPVE L+ F+ +YFD AG DL++ E VDFV  P GFL  VEN EVR W  ++H 
Sbjct: 130 ESHDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHG 189

Query: 141 FCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKMYDTA 200
             +NLS RVSD +    D HTLLPLPEPV++PGSRFREVYYWDSYW+I+GL+ S+M+ TA
Sbjct: 190 LWRNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTA 249

Query: 201 KGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALIK 260
           KG+V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E V  A+P L+K
Sbjct: 250 KGLVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLK 309

Query: 261 EHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKKAS 320
           E++FWNSG H + +R A G   +H LSRYYAMWN+PRPESS+ DE+ AS F    EK+  
Sbjct: 310 EYEFWNSGKHKVVIRDANG--YDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRF 369

Query: 321 IPGNRIGSQIWLGFQFK-MDEVQHY-------------------MELDISNLARAVGDYC 380
                  ++    F  + M +  ++                   MELDI+ + +  GD  
Sbjct: 370 HRDIATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQN 429

Query: 381 TAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFIL 440
            ++ F +AS  R+K   ++FWN + GQWLDYWL + S + + TW A NQN NV+ASNF  
Sbjct: 430 GSDRFVKASKAREKAFQTVFWNEKAGQWLDYWL-SSSGEESETWKAENQNTNVFASNFAP 489

Query: 441 LWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGWAPIQHMIVEG 500
           +WI S  SD   + KV+ +L+NSGL+  AGI TS+ NSG+QW  D PNGWAP Q MIV G
Sbjct: 490 IWINSINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQW--DSPNGWAPQQEMIVTG 549

Query: 501 LARSGMKEARALAEDIATRWLRTNY------------------------------TGFGW 528
           L RS +KEA+ +AEDIA RW+++NY                              TGFGW
Sbjct: 550 LGRSSVKEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGW 609

BLAST of Lsi09G001730 vs. Swiss-Prot
Match: TRE_ORYSJ (Probable trehalase OS=Oryza sativa subsp. japonica GN=Os10g0521000 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.8e-144
Identity = 275/573 (47.99%), Postives = 363/573 (63.35%), Query Frame = 1

Query: 16  SPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLKFDLNSTQTAFDAL 75
           +P  A  G  V    L+  L+R+Q  AL  FG  DFD K YVDL L  D  S   A  +L
Sbjct: 2   APTAAVAGGGVEAEALLGLLQRVQSEALRAFGPNDFDPKLYVDLPLAADA-SAAAALASL 61

Query: 76  ERSSNDSVPVENLKAFITDYFDSAGTDLVYS-ESVDFVPRPVGFLPKVENAEVRAWGLDI 135
            R++     +E   A+I+ YF  AG+DLV + +  DF   P GFLP+VE AE RAW L++
Sbjct: 62  PRAAPSRGEME---AYISRYFALAGSDLVAAADPPDFERDPPGFLPRVERAEARAWALEV 121

Query: 136 HNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKMYD 195
           H   K+L+RRV+  +  RPD HTLLPLP  VVVPGSRFREVYYWDSYW++RGLLVSKMY+
Sbjct: 122 HALWKDLTRRVAPAVAARPDRHTLLPLPGRVVVPGSRFREVYYWDSYWVVRGLLVSKMYE 181

Query: 196 TAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPAL 255
           TAK IV+NL+ +++++G VLNGAR+YYTNRSQPPLLSSMV DIY+ TGD+ FV    P+L
Sbjct: 182 TAKDIVLNLVYLVEKYGFVLNGARSYYTNRSQPPLLSSMVLDIYMATGDMAFVRRVFPSL 241

Query: 256 IKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKK 315
           +KEH FW S  H++ V    G    H+LSRY AMWN+PRPES+ +DE+ ASK     ++K
Sbjct: 242 LKEHSFWMSEVHNVAVMDNHG--RVHNLSRYQAMWNKPRPESATIDEEFASKLSTAAKEK 301

Query: 316 ASIPGNRIGSQIWLGFQFKMDEVQHY----------------------MELDISNLARAV 375
                +++ S    G+ F    ++                        ME DI+  A+ +
Sbjct: 302 FY---HQVASTAETGWDFSSRWMRDSTDMTTLTTSCIIPVDLNTFILKMEQDIAFFAKLI 361

Query: 376 GDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLD-NGSYKGAHTWDARNQNQNVYA 435
           G+  T+E F EAS  R   I+S+ WN++  QWLDYWL  +G+ +G + W + +QN+ ++A
Sbjct: 362 GESTTSEIFSEASKARHNAIDSVLWNADMEQWLDYWLPTDGNCQGVYQWKSISQNRAIFA 421

Query: 436 SNFILLWIESFYS------DNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNG 495
           SNF+ LW+ + +S      D  + V+V++SL+ SGLL  AGIATS+ N+G+QW  DFPNG
Sbjct: 422 SNFVPLWLNAQHSGLEQFVDEAKSVRVMRSLQKSGLLQPAGIATSLSNTGQQW--DFPNG 481

Query: 496 WAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY------------------------ 529
           WAP+QH+IVEGL RSG  EAR LAEDIATRW+RTNY                        
Sbjct: 482 WAPLQHLIVEGLLRSGSGEARELAEDIATRWVRTNYDAYKATGAMHEKYDVVTCGKSGGG 541

BLAST of Lsi09G001730 vs. Swiss-Prot
Match: TREA_HUMAN (Trehalase OS=Homo sapiens GN=TREH PE=1 SV=2)

HSP 1 Score: 268.5 bits (685), Expect = 1.6e-70
Identity = 166/530 (31.32%), Postives = 256/530 (48.30%), Query Frame = 1

Query: 48  KLDFDLKYYVDLSLKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSE 107
           KL  D K +VD+ L          F  L R  N S+P E L+AF+ ++F + G +L    
Sbjct: 46  KLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWT 105

Query: 108 SVDFVPRPVGFLPKVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVV 167
             D+   P  FL K+ +A++RAW   +H   K L +++  +++  P+  +L+    P +V
Sbjct: 106 PADWKDSPQ-FLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEHPFIV 165

Query: 168 PGSRFREVYYWDSYWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQP 227
           PG RF E YYWDSYW++ GLL+S+M +T KG++ N + ++  +GHV NG R YY  RSQP
Sbjct: 166 PGGRFVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVKTYGHVPNGGRVYYLQRSQP 225

Query: 228 PLLSSMVYDIYL-RTGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYY 287
           PLL+ M+ D YL  T D  F+   +  L  E  FW     + TV  +  G +N+ L+RYY
Sbjct: 226 PLLTLMM-DCYLTHTNDTAFLQENIETLALELDFWTK---NRTVSVSLEG-KNYLLNRYY 285

Query: 288 AMWNEPRPESSLLDEKLA--------------------------SKFVNNYEKKASIPGN 347
             +  PRPES   D +LA                          S+++       S+ G 
Sbjct: 286 VPYGGPRPESYSKDVELADTLPEGDREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGI 345

Query: 348 RIGSQIWLGFQFKMDEVQHYMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSE 407
           R    + +     + + +  M    SN    +G+   A  +      R   +N++ W+ +
Sbjct: 346 RTSKLVPVDLNAFLCQAEELM----SNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQ 405

Query: 408 KGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSG 467
            G W DY L+            + +N+  Y SN   LW   F SD     K LK L ++ 
Sbjct: 406 TGAWFDYDLEK-----------KKKNREFYPSNLTPLWAGCF-SDPGVADKALKYLEDNR 465

Query: 468 LLC-NAGIATSMINSGEQWLVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRT 520
           +L    GI TS+  +G+QW  DFPN WAP+Q +++ GLA++ ++ A+ +A  +A  W+RT
Sbjct: 466 ILTYQYGIPTSLQKTGQQW--DFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRT 525

BLAST of Lsi09G001730 vs. Swiss-Prot
Match: TREA_RABIT (Trehalase OS=Oryctolagus cuniculus GN=TREH PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.6e-70
Identity = 162/520 (31.15%), Postives = 253/520 (48.65%), Query Frame = 1

Query: 52  DLKYYVDLSLKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDF 111
           D K +VD+ L    +    +F  L  + N++VP E L+ F+ ++F + G +L      D+
Sbjct: 50  DDKQFVDMPLSTAPDQVLQSFAELAATYNNTVPREQLEKFVQEHFQAVGQELESWTPGDW 109

Query: 112 VPRPVGFLPKVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSR 171
              P  FL K+ + ++RAW   +H   K L +++  +++ +P+  +L+    P +VPG R
Sbjct: 110 KESPQ-FLQKISDPKLRAWAEQLHLLWKKLGKKIKPEVLSQPERFSLIYSQHPFIVPGGR 169

Query: 172 FREVYYWDSYWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 231
           F E YYWDSYW++ GLL+S+M +T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 170 FVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVTAYGHIPNGGRVYYLQRSQPPLLT 229

Query: 232 SMVYDIYLRTGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNE 291
            M+      TGDL F+   +  L  E  FW     + T+  + GGN +H+L+RY+  +  
Sbjct: 230 LMMDRYVAHTGDLAFLRENIETLALELDFW---AENRTISVSSGGN-SHTLNRYHVPYGG 289

Query: 292 PRPESSLLDEKLASKFVNN------YEKKASIPGNRIGSQIWLGFQFKMDEV-----QHY 351
           PRPES   D +LA             E KA        S  WL      D +        
Sbjct: 290 PRPESYSKDTELAHTLPEGSWETLWAELKAGAESGWDFSSRWLVGSPNPDSLGSIRTSKL 349

Query: 352 MELD-----------ISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWL 411
           + +D           +S     +G+   A  +      R   + ++ W+ +KG W DY L
Sbjct: 350 VPVDLNAFLCQAEELLSGFYSRLGNESQATKYRNLRAQRIAALTALLWDEDKGAWFDYDL 409

Query: 412 DNGSYKGAHTWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNA-GIA 471
           +N           + +N   Y SN   LW   F SD     K L+ L++S +L +  GI 
Sbjct: 410 EN-----------QKKNHEFYPSNLTPLWAGCF-SDPAIADKALQYLQDSQILNHRHGIP 469

Query: 472 TSMINSGEQWLVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY------- 520
           TS+ N+G+QW  DFPN WAP+Q +++ GLA+S     + +A  +A  W+RTN+       
Sbjct: 470 TSLQNTGQQW--DFPNAWAPLQDLVIRGLAKSPSARTQEVAFQLAQNWIRTNFDVYSQRS 529

BLAST of Lsi09G001730 vs. Swiss-Prot
Match: TREA_MOUSE (Trehalase OS=Mus musculus GN=Treh PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.6e-68
Identity = 160/528 (30.30%), Postives = 244/528 (46.21%), Query Frame = 1

Query: 52  DLKYYVDLSLKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDF 111
           D K +VD+SL    +     F  L    N S+P E L+ F+  +F   G +L      D+
Sbjct: 47  DDKQFVDMSLATSPDEVLQKFSELATVHNHSIPKEQLQEFVQSHFQPVGQELQSWTPEDW 106

Query: 112 VPRPVGFLPKVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSR 171
              P  FL K+ +A +R W  ++H   K L +++  +++  P+  +L+    P +VPG R
Sbjct: 107 KDSPQ-FLQKISDANLRVWAEELHKIWKKLGKKMKAEVLSYPERSSLIYSKHPFIVPGGR 166

Query: 172 FREVYYWDSYWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 231
           F E YYWDSYW++ GLL+S+M  T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 167 FVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLT 226

Query: 232 SMVYDIYLRTGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNE 291
            M+      T D+ F+   +  L  E  FW     +++V S   G +++ L+RYY  +  
Sbjct: 227 LMMDRYVAHTKDVAFLQENIGTLASELDFWTVN-RTVSVVS---GGQSYVLNRYYVPYGG 286

Query: 292 PRPESSLLDEKLASKFVNN------YEKKASIPGNRIGSQIWL----------------- 351
           PRPES   D +LA+            E KA        S  WL                 
Sbjct: 287 PRPESYRKDAELANSVPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKM 346

Query: 352 ------GFQFKMDEVQHYMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKG 411
                  F  + +E+       +SN    +G+   A  +      R   + ++ W+ +KG
Sbjct: 347 VPADLNAFLCQAEEL-------MSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKG 406

Query: 412 QWLDYWLDNGSYKGAHTWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLL 471
            W DY L+ G            +N   Y SN   LW   F SD     K LK L +S +L
Sbjct: 407 AWFDYDLEKG-----------KKNLEFYPSNLSPLWAGCF-SDPSVADKALKYLEDSKIL 466

Query: 472 C-NAGIATSMINSGEQWLVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY 520
               GI TS+ N+G+QW  DFPN WAP+Q +++ GLA+S     + +A  +A  W++TN+
Sbjct: 467 TYQYGIPTSLRNTGQQW--DFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNF 526

BLAST of Lsi09G001730 vs. TrEMBL
Match: A0A0A0KMF2_CUCSA (Trehalase OS=Cucumis sativus GN=Csa_5G149880 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.1e-255
Identity = 447/580 (77.07%), Postives = 473/580 (81.55%), Query Frame = 1

Query: 1   MPLLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLS 60
           MPLLRPAA  LPEV SPR+AD+GPV+PV NLVKFLER+QVVALN FGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERIQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLP 120
           LKFDLNSTQ AFDALERSSN SV VENL+ FI++YFDSAGTDLVYS   DF P P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTDLVYSNPADFDPHPDGFLP 144

Query: 121 KVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDS 180
           KVEN EVRAW  DIHNF KNLSRRVSDDLIH PDTHTLLPLPEPVVVPGSRFRE+YYWDS
Sbjct: 145 KVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLL SKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHSITV++   GN NHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSITVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 EKLASKFVNNYEKKASIPGNRIGSQIWLGFQFKMDEVQH--------------------- 360
           EK+ASKFVNNYEKK       I S    G+ F    ++                      
Sbjct: 325 EKVASKFVNNYEKKHLY--REIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFI 384

Query: 361 -YMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 420
             MELDISNLARAVGDYCTAEHFFEAS+VRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH
Sbjct: 385 LKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 444

Query: 421 TWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQW 480
           +WD RNQNQNVYASNFI LW+ESFYSD+++M KVLKSLRNSGLLCNAGIATSMINSGEQW
Sbjct: 445 SWDVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQW 504

Query: 481 LVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY----------------- 529
             DFPNGWAPIQHMIVEGLARS + EA+ALAEDIA RWLRTNY                 
Sbjct: 505 --DFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQK 564

BLAST of Lsi09G001730 vs. TrEMBL
Match: M5WD65_PRUPE (Trehalase OS=Prunus persica GN=PRUPE_ppa003514mg PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.5e-181
Identity = 321/569 (56.41%), Postives = 398/569 (69.95%), Query Frame = 1

Query: 13  EVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLKFDLNSTQTAF 72
           + SS   ++ GPV P   LV F +RLQ  AL  FGK  FD K+YVDLSLKF+L+ TQTAF
Sbjct: 6   KASSACTSEPGPVKPTIPLVAFFDRLQETALATFGKTKFDPKFYVDLSLKFELSKTQTAF 65

Query: 73  DALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVRAWGL 132
           D L +S+N S  V++LK FI +YF+ AG D+V +E  DFVP P GFLPKV+N EVRAW L
Sbjct: 66  DQLPKSANGSASVKDLKEFIAEYFEDAGEDMVLAEPEDFVPEPEGFLPKVKNPEVRAWAL 125

Query: 133 DIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKM 192
           ++H   KNLSR+VSD +  RP+ HTLLPLP+  V+PGSRFREVYYWDSYW+IRGLL SKM
Sbjct: 126 EVHLLWKNLSRKVSDGVHKRPEFHTLLPLPQQCVIPGSRFREVYYWDSYWVIRGLLASKM 185

Query: 193 YDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALP 252
           YDTAK IV NLI +I+E+G+VLNGARAYYTNRSQPPLLS+MV+++Y RTGD+EF   +LP
Sbjct: 186 YDTAKAIVSNLIYLIEEYGYVLNGARAYYTNRSQPPLLSAMVFEVYKRTGDVEFARKSLP 245

Query: 253 ALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYE 312
           ALIKEH+FWNSG H +TV+ +    + H+LSRYYAMWN+PRPESS +D++ AS   N YE
Sbjct: 246 ALIKEHEFWNSGIHKVTVQDSQA--QKHTLSRYYAMWNKPRPESSTIDKEFASNISNVYE 305

Query: 313 KKASIPGNRIGSQIWLGFQFKMDEVQHY----------------------MELDISNLAR 372
           K+       + S    G+ F    ++++                      ME DI+ LA+
Sbjct: 306 KQHFY--REVASAAESGWDFSTRWMRNHSDFTTLATTSILPVDLNAFILRMEHDIALLAK 365

Query: 373 AVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKG-AHTWDARNQNQNV 432
             GD+  AE F +AS  R + I ++FWN+EKGQWLDYWL N +    A TW+A NQNQNV
Sbjct: 366 VTGDHNIAERFLKASEARHEAIKTVFWNAEKGQWLDYWLGNSTCNAEAQTWEACNQNQNV 425

Query: 433 YASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGWAPI 492
           +ASNF+ LWIE F+SD   + KV +SL++SGLLC+AGIATS+  SGEQW  DFPNGWAPI
Sbjct: 426 FASNFVPLWIEPFFSDASLVEKVTRSLQSSGLLCDAGIATSLTKSGEQW--DFPNGWAPI 485

Query: 493 QHMIVEGLARSGMKEARALAEDIATRWLRTNY---------------------------- 529
           QHMIVEGLARSG+KEA+ +AEDIA RW+RTNY                            
Sbjct: 486 QHMIVEGLARSGLKEAKLVAEDIAVRWIRTNYVAYKKTGTMHEKYDVEKCGAFGGGGEYI 545

BLAST of Lsi09G001730 vs. TrEMBL
Match: W0D848_CAMSI (Trehalase OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 5.5e-179
Identity = 318/565 (56.28%), Postives = 391/565 (69.20%), Query Frame = 1

Query: 16  SPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLKFDLNSTQTAFDAL 75
           SP  ++ GPVV  T L+ FLE LQ+ ALN  G  +FD K YVDLSLK +L+ TQ AFD L
Sbjct: 35  SPTCSNSGPVVRKTPLITFLEILQLTALNTLGPKNFDPKLYVDLSLKSNLSITQNAFDDL 94

Query: 76  ERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVRAWGLDIH 135
            R+ N SV V +   FI  YF+ AG DLVY+E VDFVP P GFLP+V N EVRAW L++H
Sbjct: 95  PRTPNGSVSVTDFNGFIQSYFNGAGEDLVYTEPVDFVPEPEGFLPEVVNREVRAWALEVH 154

Query: 136 NFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKMYDT 195
           +  KNLSR+VSD ++ RP+ HTLLPL +PV++PGSRFREVYYWDSYW+IRGLL SKMY+T
Sbjct: 155 SLWKNLSRKVSDRVLDRPEFHTLLPLAKPVMIPGSRFREVYYWDSYWVIRGLLASKMYET 214

Query: 196 AKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALI 255
           AKGIV NL+S+IDEFGHVLNGARAYYTNRSQPPLLS+MVY+IY RTGD++F   +LP L+
Sbjct: 215 AKGIVTNLVSLIDEFGHVLNGARAYYTNRSQPPLLSAMVYEIYTRTGDIDFARKSLPVLL 274

Query: 256 KEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKKA 315
           KEHKFWNS  HS+ +  A G   N +LSRYYAMWN+PRPESS +D + ASK +N+  K+ 
Sbjct: 275 KEHKFWNSEIHSVIIHDAEGC--NRTLSRYYAMWNKPRPESSTIDIETASKLMNDSAKQH 334

Query: 316 SIPGNRIGSQIWLGFQFKMDEVQH----------------------YMELDISNLARAVG 375
                 + S    G+ F    +++                       MELDI+ LA   G
Sbjct: 335 LY--RELASTAESGWDFSTRWMRNGYDLTTLATTSIIPVDLNAFILKMELDIAFLANVTG 394

Query: 376 DYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASN 435
           D   + HF EA+  RK+ +NS+FWN+E GQW DYWL NG+    HTW+A NQN+N++ASN
Sbjct: 395 DCTISVHFQEAAQARKQGMNSVFWNAEMGQWFDYWLSNGTTCKGHTWEASNQNRNIFASN 454

Query: 436 FILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGWAPIQHMI 495
           F+ LWIE F SD   + +V+++LR+SGL+   GIATS+ NSG+QW  DFPNGWAP+QHMI
Sbjct: 455 FVPLWIELFNSDCTLVEQVMQNLRSSGLIRAVGIATSLTNSGQQW--DFPNGWAPLQHMI 514

Query: 496 VEGLARSGMKEARALAEDIATRWLRTNY------------------------------TG 529
           VEGLARSG KEAR++AEDIA RW+RTNY                              TG
Sbjct: 515 VEGLARSGSKEARSMAEDIAVRWIRTNYVAYKKSGAMHEKYDVEKCGEFGGGGEYIPQTG 574

BLAST of Lsi09G001730 vs. TrEMBL
Match: B9GXU4_POPTR (Trehalase OS=Populus trichocarpa GN=POPTR_0003s14400g PE=3 SV=2)

HSP 1 Score: 634.0 bits (1634), Expect = 1.6e-178
Identity = 329/582 (56.53%), Postives = 399/582 (68.56%), Query Frame = 1

Query: 1   MPLLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLS 60
           + LL  A M L    SP+ +  GPVV  T LV FLER+Q  AL  FG+ DFD K YVDLS
Sbjct: 22  LSLLSLATMTLATSLSPKCSVGGPVVATTPLVTFLERVQETALGTFGEEDFDPKLYVDLS 81

Query: 61  LKFDLNSTQTAFDALERSS-NDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFL 120
           LKF+L+ TQ AFD L RS  N +V VE+LK FI  YFD A  DLVY + VDFVP P GFL
Sbjct: 82  LKFNLSKTQKAFDELPRSGENGTVSVEDLKVFIATYFDDAADDLVYYDPVDFVPEPEGFL 141

Query: 121 PKVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWD 180
           PKV+N EVR+W L++H   KNLSR+VSD ++   + HTLLPLPE VVVPGSRFREVYYWD
Sbjct: 142 PKVKNPEVRSWALEVHALWKNLSRKVSDGVLEHSELHTLLPLPEAVVVPGSRFREVYYWD 201

Query: 181 SYWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYL 240
           SYW+IRGL+ SKMY+TAK IV NLI ++D +G+VLNGARAYYTNRSQPPLLS+MVY+IY 
Sbjct: 202 SYWVIRGLMASKMYETAKAIVTNLIFLVDTYGYVLNGARAYYTNRSQPPLLSAMVYEIYN 261

Query: 241 RTGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLL 300
           RT D+E V  ALPAL+KEH FWNS  H +T++ A G   NH+LSRYYA+WN+PRPESS +
Sbjct: 262 RTCDVELVRKALPALLKEHAFWNSEIHKVTIQDAQG--FNHNLSRYYAIWNKPRPESSTI 321

Query: 301 DEKLASKFVNNYEKKASIPGNRIGSQIWLGFQFKMDEVQH-------------------- 360
           D++ ASKF  N EK+       + S    G+ F    +++                    
Sbjct: 322 DKESASKFFGNSEKQQFY--RDVASAAESGWDFSTRWMRNTSEFTTLSTTSILPVDLNVY 381

Query: 361 --YMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSY-KG 420
              MELDI+ LA+ +G+  T E F E +  RK  INS+FW++EKGQWLDY L NG+  K 
Sbjct: 382 ILKMELDIAFLAKILGNKSTMESFMEVAEARKNAINSVFWDAEKGQWLDYRLTNGTICKE 441

Query: 421 AHTWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGE 480
           + TW A NQNQN YASNFI LWI+ F+SD   +  V++S ++SGL+  AGIATS+INSG+
Sbjct: 442 SETWQACNQNQNAYASNFIPLWIDLFHSDTALVENVMRSFQSSGLVHAAGIATSLINSGQ 501

Query: 481 QWLVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY--------------- 529
           QW  DFPNGWAP+QHMIVEGL RSG+KEAR+LAEDIA RW++TNY               
Sbjct: 502 QW--DFPNGWAPLQHMIVEGLLRSGLKEARSLAEDIAVRWIKTNYVGYKKTGAMHEKYDV 561

BLAST of Lsi09G001730 vs. TrEMBL
Match: W9RWI4_9ROSA (Trehalase OS=Morus notabilis GN=L484_007814 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 6.7e-177
Identity = 317/572 (55.42%), Postives = 389/572 (68.01%), Query Frame = 1

Query: 11  LPEVSSPRVADR--GPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLKFDLNST 70
           L + S P+ +    GPV P T L+ FLE +Q  AL  FGK DFD K YVDLSLK DL++T
Sbjct: 28  LSQTSPPKCSPNSSGPVYPTTPLLTFLENVQETALFSFGKSDFDPKLYVDLSLKLDLSTT 87

Query: 71  QTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVR 130
             AFD L R SN SV  E+L+ F+ +YF+ AG DLVY +  DFVP P GFLPKV+N +VR
Sbjct: 88  DRAFDELHRYSNGSVSAEDLREFLREYFEGAGEDLVYVKPEDFVPEPEGFLPKVKNEQVR 147

Query: 131 AWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLL 190
            W L++H+  KNLSR+VSD+++  P+ HTLLPLP  V++PGSRFREVYYWDSYW+IRGLL
Sbjct: 148 NWALEVHSLWKNLSRKVSDEVLKNPELHTLLPLPHHVIIPGSRFREVYYWDSYWVIRGLL 207

Query: 191 VSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVS 250
            SKMYDTAK IV NLIS+I+++GHVLNGARAYYTNRSQPPLLS+MV++IY RTGDLEF  
Sbjct: 208 TSKMYDTAKAIVTNLISLIEKYGHVLNGARAYYTNRSQPPLLSAMVHEIYKRTGDLEFAR 267

Query: 251 NALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFV 310
            +LP LIKEHKFWNSG H +TV+ A G   NH+L+RYYAMWN+PRPES  +D++ AS   
Sbjct: 268 KSLPPLIKEHKFWNSGIHKVTVQDAKG--LNHTLTRYYAMWNKPRPESFTMDKEFASNIT 327

Query: 311 NNYEKKASIPGNRIGSQIWLGFQFK---MDEVQHY-------------------MELDIS 370
           +  EK+     + + S    G+ F    M    ++                   MELDI+
Sbjct: 328 SFSEKQQFY--HEVASTAESGWDFSTRWMGNPSNFTTLATTSILPVDLNAFILGMELDIA 387

Query: 371 NLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQN 430
            LA+  GD C +  F +AS  RKK + S+FWN++ GQWLDYWL N   KG   W A NQN
Sbjct: 388 LLAKVTGDSCISHRFEKASRARKKAMESVFWNAKTGQWLDYWLSNRKCKGVQKWKAWNQN 447

Query: 431 QNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGW 490
           +  +ASNFI LWIESF+SD   + KV +SL+ SGL+ +AGIATS+ NS +QW  DFPNGW
Sbjct: 448 EKTFASNFIPLWIESFHSDAPLVRKVARSLQRSGLVRDAGIATSLTNSRQQW--DFPNGW 507

Query: 491 APIQHMIVEGLARSGMKEARALAEDIATRWLRTNY------------------------- 529
           APIQHMI+EGL +SG+KEAR LAE+IA RW+RTNY                         
Sbjct: 508 APIQHMIIEGLVKSGVKEARLLAEEIAVRWIRTNYVCYKKTGNMHEKYNVEKCGYFGGGG 567

BLAST of Lsi09G001730 vs. TAIR10
Match: AT4G24040.1 (AT4G24040.1 trehalase 1)

HSP 1 Score: 533.5 bits (1373), Expect = 1.5e-151
Identity = 277/561 (49.38%), Postives = 359/561 (63.99%), Query Frame = 1

Query: 21  DRGPVVPVTNLVKFLERLQVVALNLFGKLDF-DLKYYVDLSLK--FDLNSTQTAFDALER 80
           D GPVV  T LV FL+R+Q  AL  + K    D K Y+DLSLK  + L++ ++AFD L  
Sbjct: 70  DSGPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTS 129

Query: 81  SSNDS-VPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVRAWGLDIHN 140
            S+D  VPVE L+ F+ +YFD AG DL++ E VDFV  P GFL  VEN EVR W  ++H 
Sbjct: 130 ESHDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHG 189

Query: 141 FCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKMYDTA 200
             +NLS RVSD +    D HTLLPLPEPV++PGSRFREVYYWDSYW+I+GL+ S+M+ TA
Sbjct: 190 LWRNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTA 249

Query: 201 KGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALPALIK 260
           KG+V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E V  A+P L+K
Sbjct: 250 KGLVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLK 309

Query: 261 EHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKKAS 320
           E++FWNSG H + +R A G   +H LSRYYAMWN+PRPESS+ DE+ AS F    EK+  
Sbjct: 310 EYEFWNSGKHKVVIRDANG--YDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRF 369

Query: 321 IPGNRIGSQIWLGFQFK-MDEVQHY-------------------MELDISNLARAVGDYC 380
                  ++    F  + M +  ++                   MELDI+ + +  GD  
Sbjct: 370 HRDIATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQN 429

Query: 381 TAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDARNQNQNVYASNFIL 440
            ++ F +AS  R+K   ++FWN + GQWLDYWL + S + + TW A NQN NV+ASNF  
Sbjct: 430 GSDRFVKASKAREKAFQTVFWNEKAGQWLDYWL-SSSGEESETWKAENQNTNVFASNFAP 489

Query: 441 LWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGWAPIQHMIVEG 500
           +WI S  SD   + KV+ +L+NSGL+  AGI TS+ NSG+QW  D PNGWAP Q MIV G
Sbjct: 490 IWINSINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQW--DSPNGWAPQQEMIVTG 549

Query: 501 LARSGMKEARALAEDIATRWLRTNY------------------------------TGFGW 528
           L RS +KEA+ +AEDIA RW+++NY                              TGFGW
Sbjct: 550 LGRSSVKEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGW 609

BLAST of Lsi09G001730 vs. NCBI nr
Match: gi|778699376|ref|XP_004143867.2| (PREDICTED: probable trehalase [Cucumis sativus])

HSP 1 Score: 887.9 bits (2293), Expect = 8.8e-255
Identity = 447/580 (77.07%), Postives = 473/580 (81.55%), Query Frame = 1

Query: 1   MPLLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLS 60
           MPLLRPAA  LPEV SPR+AD+GPV+PV NLVKFLER+QVVALN FGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERIQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLP 120
           LKFDLNSTQ AFDALERSSN SV VENL+ FI++YFDSAGTDLVYS   DF P P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTDLVYSNPADFDPHPDGFLP 144

Query: 121 KVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDS 180
           KVEN EVRAW  DIHNF KNLSRRVSDDLIH PDTHTLLPLPEPVVVPGSRFRE+YYWDS
Sbjct: 145 KVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLL SKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHSITV++   GN NHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSITVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 EKLASKFVNNYEKKASIPGNRIGSQIWLGFQFKMDEVQH--------------------- 360
           EK+ASKFVNNYEKK       I S    G+ F    ++                      
Sbjct: 325 EKVASKFVNNYEKKHLY--REIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFI 384

Query: 361 -YMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 420
             MELDISNLARAVGDYCTAEHFFEAS+VRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH
Sbjct: 385 LKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 444

Query: 421 TWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQW 480
           +WD RNQNQNVYASNFI LW+ESFYSD+++M KVLKSLRNSGLLCNAGIATSMINSGEQW
Sbjct: 445 SWDVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQW 504

Query: 481 LVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY----------------- 529
             DFPNGWAPIQHMIVEGLARS + EA+ALAEDIA RWLRTNY                 
Sbjct: 505 --DFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQK 564

BLAST of Lsi09G001730 vs. NCBI nr
Match: gi|659074046|ref|XP_008437392.1| (PREDICTED: probable trehalase [Cucumis melo])

HSP 1 Score: 885.2 bits (2286), Expect = 5.7e-254
Identity = 446/580 (76.90%), Postives = 474/580 (81.72%), Query Frame = 1

Query: 1   MPLLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLS 60
           MPLLRPAA  LPEV SPR+AD+GPV+PV NLVKFLERLQVVALN FGKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLP 120
           LKFDLNSTQ AFDALERSSN SV VENL+ FI++YF SAGTDLVYS  VDF P+P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 144

Query: 121 KVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDS 180
           KVEN EVR W LDIHNF KNLSRRVSDDLIH PDTHTLLPLPEPVVVPGSRFRE+YYWDS
Sbjct: 145 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLL SKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLD 300
           TGDLEFV N+LPALIKEHKFWNSGFHS+TV++   GN NHSLSRYYAMWNEPRPESSL+D
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQN---GNGNHSLSRYYAMWNEPRPESSLVD 324

Query: 301 EKLASKFVNNYEKKASIPGNRIGSQIWLGFQFKMDEVQH--------------------- 360
           EKLASKF NNYEKK       I S    G+ F    ++                      
Sbjct: 325 EKLASKFANNYEKKHLY--REIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFI 384

Query: 361 -YMELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 420
             MELDISNLARAVGDYCTAEHFFEAS+VRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH
Sbjct: 385 LKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 444

Query: 421 TWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQW 480
           +W+ARNQNQN+YASNFI LWIESFYSD+++M KVLKSLRNSGLLCNAGIATS+INSGEQW
Sbjct: 445 SWNARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQW 504

Query: 481 LVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY----------------- 529
             DFPNGWAPIQHMIVEGLARS + EA+ALAEDIA RWLRTNY                 
Sbjct: 505 --DFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQK 564

BLAST of Lsi09G001730 vs. NCBI nr
Match: gi|595857250|ref|XP_007210503.1| (hypothetical protein PRUPE_ppa003514mg [Prunus persica])

HSP 1 Score: 644.0 bits (1660), Expect = 2.2e-181
Identity = 321/569 (56.41%), Postives = 398/569 (69.95%), Query Frame = 1

Query: 13  EVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLKFDLNSTQTAF 72
           + SS   ++ GPV P   LV F +RLQ  AL  FGK  FD K+YVDLSLKF+L+ TQTAF
Sbjct: 6   KASSACTSEPGPVKPTIPLVAFFDRLQETALATFGKTKFDPKFYVDLSLKFELSKTQTAF 65

Query: 73  DALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKVENAEVRAWGL 132
           D L +S+N S  V++LK FI +YF+ AG D+V +E  DFVP P GFLPKV+N EVRAW L
Sbjct: 66  DQLPKSANGSASVKDLKEFIAEYFEDAGEDMVLAEPEDFVPEPEGFLPKVKNPEVRAWAL 125

Query: 133 DIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLVSKM 192
           ++H   KNLSR+VSD +  RP+ HTLLPLP+  V+PGSRFREVYYWDSYW+IRGLL SKM
Sbjct: 126 EVHLLWKNLSRKVSDGVHKRPEFHTLLPLPQQCVIPGSRFREVYYWDSYWVIRGLLASKM 185

Query: 193 YDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVSNALP 252
           YDTAK IV NLI +I+E+G+VLNGARAYYTNRSQPPLLS+MV+++Y RTGD+EF   +LP
Sbjct: 186 YDTAKAIVSNLIYLIEEYGYVLNGARAYYTNRSQPPLLSAMVFEVYKRTGDVEFARKSLP 245

Query: 253 ALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYE 312
           ALIKEH+FWNSG H +TV+ +    + H+LSRYYAMWN+PRPESS +D++ AS   N YE
Sbjct: 246 ALIKEHEFWNSGIHKVTVQDSQA--QKHTLSRYYAMWNKPRPESSTIDKEFASNISNVYE 305

Query: 313 KKASIPGNRIGSQIWLGFQFKMDEVQHY----------------------MELDISNLAR 372
           K+       + S    G+ F    ++++                      ME DI+ LA+
Sbjct: 306 KQHFY--REVASAAESGWDFSTRWMRNHSDFTTLATTSILPVDLNAFILRMEHDIALLAK 365

Query: 373 AVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKG-AHTWDARNQNQNV 432
             GD+  AE F +AS  R + I ++FWN+EKGQWLDYWL N +    A TW+A NQNQNV
Sbjct: 366 VTGDHNIAERFLKASEARHEAIKTVFWNAEKGQWLDYWLGNSTCNAEAQTWEACNQNQNV 425

Query: 433 YASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDFPNGWAPI 492
           +ASNF+ LWIE F+SD   + KV +SL++SGLLC+AGIATS+  SGEQW  DFPNGWAPI
Sbjct: 426 FASNFVPLWIEPFFSDASLVEKVTRSLQSSGLLCDAGIATSLTKSGEQW--DFPNGWAPI 485

Query: 493 QHMIVEGLARSGMKEARALAEDIATRWLRTNY---------------------------- 529
           QHMIVEGLARSG+KEA+ +AEDIA RW+RTNY                            
Sbjct: 486 QHMIVEGLARSGLKEAKLVAEDIAVRWIRTNYVAYKKTGTMHEKYDVEKCGAFGGGGEYI 545

BLAST of Lsi09G001730 vs. NCBI nr
Match: gi|645267694|ref|XP_008239192.1| (PREDICTED: probable trehalase isoform X2 [Prunus mume])

HSP 1 Score: 642.1 bits (1655), Expect = 8.4e-181
Identity = 321/580 (55.34%), Postives = 402/580 (69.31%), Query Frame = 1

Query: 1   MPLLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLS 60
           + LL P      + SS   ++ GPV P   LV F +RLQ  AL  FGK +FD K+YVDLS
Sbjct: 22  LSLLLPTMTFTTKASSACTSEPGPVKPTIPLVAFFDRLQETALATFGKTNFDPKFYVDLS 81

Query: 61  LKFDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLP 120
           LKF+L+ TQ AFD L +S+N S  V++LK FI +YF+ AG D+V +E  DFVP P GFLP
Sbjct: 82  LKFELSKTQKAFDQLPKSANGSASVKDLKEFIAEYFEDAGEDMVLAEPEDFVPEPEGFLP 141

Query: 121 KVENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDS 180
           KV+N EVRAW L++H   KNLSR+VSD++  RP+ HTLLPLP+  V+PGSRFREVYYWDS
Sbjct: 142 KVKNPEVRAWALEVHLLWKNLSRKVSDEVHKRPEFHTLLPLPQQCVIPGSRFREVYYWDS 201

Query: 181 YWIIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YW+IRGLL SKMY+TAK IV NLI +I+E+G+VLNGARAYYTNRSQPPLLS+MV+++Y R
Sbjct: 202 YWVIRGLLASKMYNTAKAIVSNLIYLIEEYGYVLNGARAYYTNRSQPPLLSAMVFEVYQR 261

Query: 241 TGDLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLD 300
           TGD+EF   +LPALIKEH+FWNSG H +TVR +    + H+LSRYYAMWN+PRPESS +D
Sbjct: 262 TGDVEFARKSLPALIKEHEFWNSGIHKVTVRDSQA--QKHTLSRYYAMWNKPRPESSTID 321

Query: 301 EKLASKFVNNYEKKASIPGNRIGSQIWLGFQFKMDEVQHY-------------------- 360
           ++ AS   N  EK+       + S    G+ F    ++++                    
Sbjct: 322 KEFASNISNVDEKQHFY--REVASAAESGWDFSTRWMRNHSDFTTLATTSILPVDLNAFI 381

Query: 361 --MELDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAH 420
             ME DI+ LA+  GD+  AE F +AS  R + I ++FWN+EKGQWLDYWL N +   A 
Sbjct: 382 LRMEHDIALLAKVTGDHNIAECFLKASEARHEAIKTVFWNAEKGQWLDYWLGNSTCNEAQ 441

Query: 421 TWDARNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQW 480
           TW+A NQNQNV+ASNF+ LW+E F+SD   + KV +SL++SGLLC+AGIATS+  SGEQW
Sbjct: 442 TWEACNQNQNVFASNFVPLWVEPFFSDASLVEKVTRSLQSSGLLCDAGIATSLTKSGEQW 501

Query: 481 LVDFPNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY----------------- 529
             DFPNGWAPIQHMIVEGLARSG+KEA+ +AEDIA RW+RTNY                 
Sbjct: 502 --DFPNGWAPIQHMIVEGLARSGLKEAKLVAEDIAVRWIRTNYVAYKKTGTMHEKYDVEK 561

BLAST of Lsi09G001730 vs. NCBI nr
Match: gi|1009156021|ref|XP_015896023.1| (PREDICTED: probable trehalase [Ziziphus jujuba])

HSP 1 Score: 641.7 bits (1654), Expect = 1.1e-180
Identity = 328/576 (56.94%), Postives = 400/576 (69.44%), Query Frame = 1

Query: 3   LLRPAAMPLPEVSSPRVADRGPVVPVTNLVKFLERLQVVALNLFGKLDFDLKYYVDLSLK 62
           LL   A    E SSP  +D GPV P   LV FLER+Q +A   FG+ +FD K YVDLSLK
Sbjct: 20  LLLLIATDSTEASSPSNSDPGPVKPTAPLVSFLERVQQLAFYTFGESNFDPKLYVDLSLK 79

Query: 63  FDLNSTQTAFDALERSSNDSVPVENLKAFITDYFDSAGTDLVYSESVDFVPRPVGFLPKV 122
           F+L   + AFD L RS N  V V++L+ +I  YF++AG D+VY+E  DFVP PVGFLPKV
Sbjct: 80  FNLEDAEKAFDKLPRSGNGKVSVKDLRGYIGKYFEAAGDDVVYAEPEDFVPEPVGFLPKV 139

Query: 123 ENAEVRAWGLDIHNFCKNLSRRVSDDLIHRPDTHTLLPLPEPVVVPGSRFREVYYWDSYW 182
           +N EVRAW L++H+  KNLSRRVS +    P+ HTLLPLP+ V++PGSRFREVYYWDSYW
Sbjct: 140 KNQEVRAWALEVHSLWKNLSRRVSSETQKHPELHTLLPLPKQVIIPGSRFREVYYWDSYW 199

Query: 183 IIRGLLVSKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTG 242
           IIRGLL SKM+DTAK IV NLIS+I+E+G VLNGARAYY+NRS+PPLLS+MVY+IY RTG
Sbjct: 200 IIRGLLASKMHDTAKAIVTNLISLIEEYGFVLNGARAYYSNRSEPPLLSAMVYEIYKRTG 259

Query: 243 DLEFVSNALPALIKEHKFWNSGFHSITVRSAPGGNENHSLSRYYAMWNEPRPESSLLDEK 302
           DLEF    LPALIKEH+FWNS  H +T++ A      H+LSRYYAMWNEPRPESS LDEK
Sbjct: 260 DLEFAKKCLPALIKEHQFWNSEIHKVTIQDAQACT--HTLSRYYAMWNEPRPESSTLDEK 319

Query: 303 LASKFVNNYEKK-------ASIPGNRIGSQIWLG-------------FQFKMDEVQHYME 362
            ASK + + EKK       ++       S  W+                  ++     ME
Sbjct: 320 FASK-IPDVEKKHFFREVASTAESGWDFSTRWMRNTTDFTTLATTSILPADLNAFILGME 379

Query: 363 LDISNLARAVGDYCTAEHFFEASIVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHTWDA 422
           L+I++LA+A+GDY  ++HF +AS  R+K I S+FWN+EKGQWLDYWL NG++K A  W+A
Sbjct: 380 LNIASLAKAIGDYNISKHFLKASQAREKAIRSVFWNAEKGQWLDYWLANGTWKEAEKWEA 439

Query: 423 RNQNQNVYASNFILLWIESFYSDNKRMVKVLKSLRNSGLLCNAGIATSMINSGEQWLVDF 482
           RNQN  V+ASNFI LWI+SFYSD   +  V +SL++SGLL + GIATS+  SG+QW  DF
Sbjct: 440 RNQNPKVFASNFIPLWIKSFYSDTILVDSVRRSLQSSGLLRSVGIATSLTKSGQQW--DF 499

Query: 483 PNGWAPIQHMIVEGLARSGMKEARALAEDIATRWLRTNY--------------------- 529
           PNGWAP+QHMIVEGLARSG K+AR+LA DIA  W+RTN+                     
Sbjct: 500 PNGWAPLQHMIVEGLARSGSKQARSLAMDIAVGWIRTNHVAYKITSAMHEKYNVEKCGDF 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TRE1_ARATH2.6e-15049.38Trehalase OS=Arabidopsis thaliana GN=TRE1 PE=2 SV=1[more]
TRE_ORYSJ4.8e-14447.99Probable trehalase OS=Oryza sativa subsp. japonica GN=Os10g0521000 PE=2 SV=1[more]
TREA_HUMAN1.6e-7031.32Trehalase OS=Homo sapiens GN=TREH PE=1 SV=2[more]
TREA_RABIT1.6e-7031.15Trehalase OS=Oryctolagus cuniculus GN=TREH PE=1 SV=1[more]
TREA_MOUSE5.6e-6830.30Trehalase OS=Mus musculus GN=Treh PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMF2_CUCSA6.1e-25577.07Trehalase OS=Cucumis sativus GN=Csa_5G149880 PE=3 SV=1[more]
M5WD65_PRUPE1.5e-18156.41Trehalase OS=Prunus persica GN=PRUPE_ppa003514mg PE=3 SV=1[more]
W0D848_CAMSI5.5e-17956.28Trehalase OS=Camellia sinensis PE=2 SV=1[more]
B9GXU4_POPTR1.6e-17856.53Trehalase OS=Populus trichocarpa GN=POPTR_0003s14400g PE=3 SV=2[more]
W9RWI4_9ROSA6.7e-17755.42Trehalase OS=Morus notabilis GN=L484_007814 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24040.11.5e-15149.38 trehalase 1[more]
Match NameE-valueIdentityDescription
gi|778699376|ref|XP_004143867.2|8.8e-25577.07PREDICTED: probable trehalase [Cucumis sativus][more]
gi|659074046|ref|XP_008437392.1|5.7e-25476.90PREDICTED: probable trehalase [Cucumis melo][more]
gi|595857250|ref|XP_007210503.1|2.2e-18156.41hypothetical protein PRUPE_ppa003514mg [Prunus persica][more]
gi|645267694|ref|XP_008239192.1|8.4e-18155.34PREDICTED: probable trehalase isoform X2 [Prunus mume][more]
gi|1009156021|ref|XP_015896023.1|1.1e-18056.94PREDICTED: probable trehalase [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0004555alpha,alpha-trehalase activity
Vocabulary: Biological Process
TermDefinition
GO:0005991trehalose metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR0089286-hairpin_glycosidase_sf
IPR001661Glyco_hydro_37
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005993 trehalose catabolic process
biological_process GO:0005991 trehalose metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004555 alpha,alpha-trehalase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G001730.1Lsi09G001730.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001661Glycoside hydrolase, family 37PRINTSPR00744GLHYDRLASE37coord: 502..515
score: 3.0E-19coord: 372..388
score: 3.0E-19coord: 282..299
score: 3.0E-19coord: 210..228
score: 3.0
IPR001661Glycoside hydrolase, family 37PANTHERPTHR23403TREHALASEcoord: 13..528
score: 4.0E
IPR001661Glycoside hydrolase, family 37PFAMPF01204Trehalasecoord: 341..501
score: 1.4E-35coord: 49..313
score: 4.8
IPR008928Six-hairpin glycosidase-likeunknownSSF48208Six-hairpin glycosidasescoord: 48..521
score: 3.0
NoneNo IPR availablePANTHERPTHR23403:SF1TREHALASEcoord: 13..528
score: 4.0E

The following gene(s) are paralogous to this gene:

None