CmoCh02G009180 (gene) Cucurbita moschata (Rifu)

NameCmoCh02G009180
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionTrehalase (3.2.1.28) (Alpha-trehalose glucohydrolase)
LocationCmo_Chr02 : 5646854 .. 5650606 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGGCCGCGCGCCGCCCGTTGTGTTTGGTACCAAATTTTGATATCAACAGGCGGAGAGAGAGAAAGAAATTGATATCCATTTGTTAATTGGCCTAATCCATTGGACACCATTCATAGCTTCTTTCCAAGATCATAAAAACGAAATTTCCATCTCGATTCTCGATTCATTCTTCTCTCATAATTTTCCCATCAATCCTCGTCATCACCTTCTTCTTCGCCACAAATCCCTCTCTCTCTCTCTCTTCTCTGTACTTTTTTTTCTCTCTCTCCTAGTCCGCACTCTGTCATCACCTCCCCCAGTTTTCTTCCTCCATAAATATGGCTTTCTTTCAACCCCTTCTTACTCCGTCCGTTCATTGTTCATTAACTCTTTTTCTCTTTTTCCTTGTCGTCGTACCATTGCTTCCTCCCGCCGCTCCCATGCCTTTGCACGAAGTTTACTCTCCTCGCTTGGCCTATAGGGGTCCGGTTATTCCGGTCAGCAATCTCGTCAAGTTTCTCGAGCGTCTTCAGGTTGTGGCGCTCAATTCCTTTGGTAAACTTGATTTCGATCTTAAATATTACGTCGATTTGTCGCTTAAATTTGATTTGAATTCCACTGAGAGGGCTTTTGATGCCCTTTCACGATCCTCCAATGGCTCTGTCCCGGTCGAGGATTTGAAGTCGTTTATTAGCGAGTATTTCGATAGTGCTGGAACCGATATGGTGTACTCCGACCCTCTGGATTTTGTCCCTCGGCCTCAAGGGTTTTTGCCGAAGGTGGAGAATGCGGGGGTTAGAGCTTGGGCTTTGGAGATTCATAATTACTGGAAGAATCTTAGTCGGAAAGTCTCCGATGATATCATTCATCGCCCCGATGCTCACACTCTGCTGCCGTTGCCTATGCCCTGTATCATTCCCGGAGCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGGTATTGTGATGGCTACAGTTTTGTCTCGATTTCTTTCTGCTTTTTCAATGATTTTATTTTAATCTCCAGCGTTTTAACTTGACTTGTACTTTTCCAAGAAAACTGTTCCTGATTGAATTTGGCCTTTTATAAACTCACTTTTTTCTTCACATTGTTCTTATCTTTGCCGGCCCCTTTTCTTGGATTCTGTGTTTCCCCCTTTTGTCACTTTGTGAAGAAACAATCCTCGGGCTGTTCAACTGGTTTCTGTCAAATATTCCACTGTGAAATTTGGGAAATGCAATTTGAAAATTTCCCATTAAGAAACTGAAAATTTTGTACATTATATTTTCTTGAATGATGACAAAACTGGGTGGGATTGTTCCCTCATTTGTTTTTATTTCATTTTGTGCAGAGGATTGTTAGCTAGTAAAATGTATGATACTGCCAAGGGAATTGTTATCAATCTCATTTCAATGATTGATGAATTTGGCCATGTTTTGAATGGTGCTAGAGCTTATTACACTAACAGAAGGTAAATTAACTCATCAATCCCTGTGAAGAATGTGTGAAAATTTAGTAGTTTTGGAAACTGATTATCCAACCCTGGACTACATTTTGCAGTCAGCCTCCTCTCTTGAGTTCCATGGTTTATGACATATACCTTAGAACAGGTGATTTAGAGTTCGTGAGGAATTCCCTCCCAGCTTTGATCAAAGAGCATATGTTCTGGAATTCAGGTACAACGAGATACCGATGCGTCTTACTCGATCGACCATTATAGCTGTATTGATTTCCTTAGTTTTAGTCAGTTTGTAATTTAGTCTTTCTCTATTGGTTTTAGGATTCCATTCTGTTACCGTCCGGAAGGCTCCTGGTGGAAATCATTCTTTGTCTAGATATTATGCAATGTGGAATGAACCCAGGCCTGAATCTTCTCTGCTGGTGTGTTTTCCTCCTGGATCTTAGTTACCTACCTGCCATAATATGAACTTACCTTGTGTTTGACTTCATGATTTATTCTTGACAGGATGAGAAACTTGCCTCAAAGTTTGTGAATAACTACGACAAGCAGCGTCTATACCGTGAACTCGCATCGGCTGCCGAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGTATAACCATTGTGATTTGGAATATCTGCAGAGCTAAGAGTTTATTAAGTTTTGAGACATTTTTATAGCATTACAGCAATGAAGTTTTTAAACGCTTTTGAGTTTGCTTCTTGAAGGGATCACACGGACTTATCAACATTGGCTACAACTTCAATCTTGCCAGTTGATCTCAATGTATTCATACTCAAGGTATGGCTTGAACAGTTCTTCGAAAACCTCGACGTTCTTCGTATCACCATTAGCACAACTAATGTTTTCTCTGAATTCAGATGGAACTTGACATCTCCAATTTGGCAAGAGCTGCTGGAGATTTCTGCCTTGCTTATAAATTTTTGGAGGCTTCACTAGTCAGAAAGAAGACAATCAACTCTATTTTCTGGAACTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCTCACAAGGTATATATATATGCATATAATTTCTGAAAACTTTCTAAGCTTTTGATACTCTTCTTTTAGCAGTTATGTTGATTGAAACTTTTGGAGCTTTCAGGGTGTTCATACATGGGATGCTCAGAACCAGAACAAGAACATATATGCTTCGAACTTCATTCCGCTCTGGATCGAATCGTTCTACAACGGTTTGTAGCTAATTCCCTCTTTATCATAAGCATTTTTTCAGCTAAGTTTCTCTGAAGGCTAAGGGTAGGAATTGTATGCTTGTTTTTGTATAAACAGACAGCATACAGATGAAGAAAGTGTTGAAAAGCTTACGGAAGTCAGGCTTGCTATGCAATGCCGGGATTGCAACTTCTACTATTAATTCAGGAGAACAATGGTTAGTAGTACTGAATCTTTAGGAACTGTTTGGTTTACATGCACAACACTCAAACCTTTCATATCTGTACACAACAAAAGGGATTTCCCGAATGGTTGGGCGCCAATTCAGCACATGATCATCGAGGGACTGGCGAGATCGGAGTTGAAAGAAGCAAGGGTATTGGCTGAGGATATGGCCGTGAGATGGCTTCGAACCAACTATGTGGCTTACAAGAAAACAGGGTATATGCATGAGAAATATGATGTCCAAAAGTGTGGAGACTTCGGCGCAGGAGGTGAATATGTCCCTCAGGTACATCACTCATCAACTTGCATTGGCATTTCTCTCTTCCACTCATTTGAATCATTAAGAAAAGAAAGTGTTCCTCTGCTGATCTGTTTCCTTTTGGTTCCTATGTTTGAGTGTAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGGCATTCCTTGAAGAGTTTGGATGGCCCGAAGATCAAAATATTGACTGCTAATTGACTAGACCACGGTGTACATGTTCGAGAGTGACTTTGGAATTGCCTAACTTGACTTTTACGATGATCCACATGACTAAACTACTTTTGGCCATAGATTCATTGAAGAGTTTGGAGGTGATTTTGAAAGATGTTTAGAAAATTAGAGAGAGGAGCCCCGTGGTTGGACGAGCCTCCATGTTAATGTAAAGATTAAGGAGTAAATATATATTAGATCCGTATTATACCATTCACATGATATCAAAATGAATGTAACGTCTTTCTAATATATTTGTCTATGCTTTTTCATGAAGTTTAAATCTCTATTACTACCGTTGTAGTACTAAAATTTGGCTCTAATATCAATTTTTAGATTTTTGTAGAGTGATTCGAATAAC

mRNA sequence

CAGGCCGCGCGCCGCCCGTTGTGTTTGGTACCAAATTTTGATATCAACAGGCGGAGAGAGAGAAAGAAATTGATATCCATTTGTTAATTGGCCTAATCCATTGGACACCATTCATAGCTTCTTTCCAAGATCATAAAAACGAAATTTCCATCTCGATTCTCGATTCATTCTTCTCTCATAATTTTCCCATCAATCCTCGTCATCACCTTCTTCTTCGCCACAAATCCCTCTCTCTCTCTCTCTTCTCTGTACTTTTTTTTCTCTCTCTCCTAGTCCGCACTCTGTCATCACCTCCCCCAGTTTTCTTCCTCCATAAATATGGCTTTCTTTCAACCCCTTCTTACTCCGTCCGTTCATTGTTCATTAACTCTTTTTCTCTTTTTCCTTGTCGTCGTACCATTGCTTCCTCCCGCCGCTCCCATGCCTTTGCACGAAGTTTACTCTCCTCGCTTGGCCTATAGGGGTCCGGTTATTCCGGTCAGCAATCTCGTCAAGTTTCTCGAGCGTCTTCAGGTTGTGGCGCTCAATTCCTTTGGTAAACTTGATTTCGATCTTAAATATTACGTCGATTTGTCGCTTAAATTTGATTTGAATTCCACTGAGAGGGCTTTTGATGCCCTTTCACGATCCTCCAATGGCTCTGTCCCGGTCGAGGATTTGAAGTCGTTTATTAGCGAGTATTTCGATAGTGCTGGAACCGATATGGTGTACTCCGACCCTCTGGATTTTGTCCCTCGGCCTCAAGGGTTTTTGCCGAAGGTGGAGAATGCGGGGGTTAGAGCTTGGGCTTTGGAGATTCATAATTACTGGAAGAATCTTAGTCGGAAAGTCTCCGATGATATCATTCATCGCCCCGATGCTCACACTCTGCTGCCGTTGCCTATGCCCTGTATCATTCCCGGAGCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGAGGATTGTTAGCTAGTAAAATGTATGATACTGCCAAGGGAATTGTTATCAATCTCATTTCAATGATTGATGAATTTGGCCATGTTTTGAATGGTGCTAGAGCTTATTACACTAACAGAAGTCAGCCTCCTCTCTTGAGTTCCATGGTTTATGACATATACCTTAGAACAGGTGATTTAGAGTTCGTGAGGAATTCCCTCCCAGCTTTGATCAAAGAGCATATGTTCTGGAATTCAGGATTCCATTCTGTTACCGTCCGGAAGGCTCCTGGTGGAAATCATTCTTTGTCTAGATATTATGCAATGTGGAATGAACCCAGGCCTGAATCTTCTCTGCTGGATGAGAAACTTGCCTCAAAGTTTGTGAATAACTACGACAAGCAGCGTCTATACCGTGAACTCGCATCGGCTGCCGAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGGATCACACGGACTTATCAACATTGGCTACAACTTCAATCTTGCCAGTTGATCTCAATGTATTCATACTCAAGATGGAACTTGACATCTCCAATTTGGCAAGAGCTGCTGGAGATTTCTGCCTTGCTTATAAATTTTTGGAGGCTTCACTAGTCAGAAAGAAGACAATCAACTCTATTTTCTGGAACTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCTCACAAGGGTGTTCATACATGGGATGCTCAGAACCAGAACAAGAACATATATGCTTCGAACTTCATTCCGCTCTGGATCGAATCGTTCTACAACGACAGCATACAGATGAAGAAAGTGTTGAAAAGCTTACGGAAGTCAGGCTTGCTATGCAATGCCGGGATTGCAACTTCTACTATTAATTCAGGAGAACAATGGGATTTCCCGAATGGTTGGGCGCCAATTCAGCACATGATCATCGAGGGACTGGCGAGATCGGAGTTGAAAGAAGCAAGGGTATTGGCTGAGGATATGGCCGTGAGATGGCTTCGAACCAACTATGTGGCTTACAAGAAAACAGGGTATATGCATGAGAAATATGATGTCCAAAAGTGTGGAGACTTCGGCGCAGGAGGTGAATATGTCCCTCAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGGCATTCCTTGAAGAGTTTGGATGGCCCGAAGATCAAAATATTGACTGCTAATTGACTAGACCACGGTGTACATGTTCGAGAGTGACTTTGGAATTGCCTAACTTGACTTTTACGATGATCCACATGACTAAACTACTTTTGGCCATAGATTCATTGAAGAGTTTGGAGGTGATTTTGAAAGATGTTTAGAAAATTAGAGAGAGGAGCCCCGTGGTTGGACGAGCCTCCATGTTAATGTAAAGATTAAGGAGTAAATATATATTAGATCCGTATTATACCATTCACATGATATCAAAATGAATGTAACGTCTTTCTAATATATTTGTCTATGCTTTTTCATGAAGTTTAAATCTCTATTACTACCGTTGTAGTACTAAAATTTGGCTCTAATATCAATTTTTAGATTTTTGTAGAGTGATTCGAATAAC

Coding sequence (CDS)

ATGGCTTTCTTTCAACCCCTTCTTACTCCGTCCGTTCATTGTTCATTAACTCTTTTTCTCTTTTTCCTTGTCGTCGTACCATTGCTTCCTCCCGCCGCTCCCATGCCTTTGCACGAAGTTTACTCTCCTCGCTTGGCCTATAGGGGTCCGGTTATTCCGGTCAGCAATCTCGTCAAGTTTCTCGAGCGTCTTCAGGTTGTGGCGCTCAATTCCTTTGGTAAACTTGATTTCGATCTTAAATATTACGTCGATTTGTCGCTTAAATTTGATTTGAATTCCACTGAGAGGGCTTTTGATGCCCTTTCACGATCCTCCAATGGCTCTGTCCCGGTCGAGGATTTGAAGTCGTTTATTAGCGAGTATTTCGATAGTGCTGGAACCGATATGGTGTACTCCGACCCTCTGGATTTTGTCCCTCGGCCTCAAGGGTTTTTGCCGAAGGTGGAGAATGCGGGGGTTAGAGCTTGGGCTTTGGAGATTCATAATTACTGGAAGAATCTTAGTCGGAAAGTCTCCGATGATATCATTCATCGCCCCGATGCTCACACTCTGCTGCCGTTGCCTATGCCCTGTATCATTCCCGGAGCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGAGGATTGTTAGCTAGTAAAATGTATGATACTGCCAAGGGAATTGTTATCAATCTCATTTCAATGATTGATGAATTTGGCCATGTTTTGAATGGTGCTAGAGCTTATTACACTAACAGAAGTCAGCCTCCTCTCTTGAGTTCCATGGTTTATGACATATACCTTAGAACAGGTGATTTAGAGTTCGTGAGGAATTCCCTCCCAGCTTTGATCAAAGAGCATATGTTCTGGAATTCAGGATTCCATTCTGTTACCGTCCGGAAGGCTCCTGGTGGAAATCATTCTTTGTCTAGATATTATGCAATGTGGAATGAACCCAGGCCTGAATCTTCTCTGCTGGATGAGAAACTTGCCTCAAAGTTTGTGAATAACTACGACAAGCAGCGTCTATACCGTGAACTCGCATCGGCTGCCGAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGGATCACACGGACTTATCAACATTGGCTACAACTTCAATCTTGCCAGTTGATCTCAATGTATTCATACTCAAGATGGAACTTGACATCTCCAATTTGGCAAGAGCTGCTGGAGATTTCTGCCTTGCTTATAAATTTTTGGAGGCTTCACTAGTCAGAAAGAAGACAATCAACTCTATTTTCTGGAACTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCTCACAAGGGTGTTCATACATGGGATGCTCAGAACCAGAACAAGAACATATATGCTTCGAACTTCATTCCGCTCTGGATCGAATCGTTCTACAACGACAGCATACAGATGAAGAAAGTGTTGAAAAGCTTACGGAAGTCAGGCTTGCTATGCAATGCCGGGATTGCAACTTCTACTATTAATTCAGGAGAACAATGGGATTTCCCGAATGGTTGGGCGCCAATTCAGCACATGATCATCGAGGGACTGGCGAGATCGGAGTTGAAAGAAGCAAGGGTATTGGCTGAGGATATGGCCGTGAGATGGCTTCGAACCAACTATGTGGCTTACAAGAAAACAGGGTATATGCATGAGAAATATGATGTCCAAAAGTGTGGAGACTTCGGCGCAGGAGGTGAATATGTCCCTCAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGGCATTCCTTGAAGAGTTTGGATGGCCCGAAGATCAAAATATTGACTGCTAA
BLAST of CmoCh02G009180 vs. Swiss-Prot
Match: TRE1_ARATH (Trehalase OS=Arabidopsis thaliana GN=TRE1 PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 3.7e-193
Identity = 317/555 (57.12%), Postives = 417/555 (75.14%), Query Frame = 1

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDF-DLKYYVDLSLK--FDLNSTERAFDAL-SRS 108
           GPV+  + LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E AFD L S S
Sbjct: 72  GPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTSES 131

Query: 109 SNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYW 168
            +  VPVE L+ F+ EYFD AG D+++ +P+DFV  P GFL  VEN  VR WA E+H  W
Sbjct: 132 HDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHGLW 191

Query: 169 KNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKG 228
           +NLS +VSD +    D HTLLPLP P IIPG+RFREVYYWDSYW+I+GL+ S+M+ TAKG
Sbjct: 192 RNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTAKG 251

Query: 229 IVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH 288
           +V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E VR ++P L+KE+
Sbjct: 252 LVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLKEY 311

Query: 289 MFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYREL 348
            FWNSG H V +R A G +H LSRYYAMWN+PRPESS+ DE+ AS F    +KQR +R++
Sbjct: 312 EFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHRDI 371

Query: 349 ASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYK 408
           A+AAESG DFS+RWMRD  + +T+ATTS++PVDLNVF+LKMELDI+ + + +GD   + +
Sbjct: 372 ATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGSDR 431

Query: 409 FLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIE 468
           F++AS  R+K   ++FWN + GQWLDYWL +   +   TW A+NQN N++ASNF P+WI 
Sbjct: 432 FVKASKAREKAFQTVFWNEKAGQWLDYWLSSSGEES-ETWKAENQNTNVFASNFAPIWIN 491

Query: 469 SFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSEL 528
           S  +D   +KKV+ +L+ SGL+  AGI TS  NSG+QWD PNGWAP Q MI+ GL RS +
Sbjct: 492 SINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRSSV 551

Query: 529 KEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVL 588
           KEA+ +AED+A RW+++NY+ YKK+G +HEK  V + G++G GGEY+PQTGFGWSNGV+L
Sbjct: 552 KEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGVIL 611

Query: 589 AFLEEFGWPEDQNID 600
           AFLEE+GWP   +I+
Sbjct: 612 AFLEEYGWPSHLSIE 625

BLAST of CmoCh02G009180 vs. Swiss-Prot
Match: TRE_ORYSJ (Probable trehalase OS=Oryza sativa subsp. japonica GN=Os10g0521000 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 3.1e-187
Identity = 315/567 (55.56%), Postives = 413/567 (72.84%), Query Frame = 1

Query: 42  SPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDAL 101
           +P  A  G  +    L+  L+R+Q  AL +FG  DFD K YVDL L  D ++      AL
Sbjct: 2   APTAAVAGGGVEAEALLGLLQRVQSEALRAFGPNDFDPKLYVDLPLAADASAAA----AL 61

Query: 102 SRSSNGSVPVEDLKSFISEYFDSAGTDMVYS-DPLDFVPRPQGFLPKVENAGVRAWALEI 161
           +     +    +++++IS YF  AG+D+V + DP DF   P GFLP+VE A  RAWALE+
Sbjct: 62  ASLPRAAPSRGEMEAYISRYFALAGSDLVAAADPPDFERDPPGFLPRVERAEARAWALEV 121

Query: 162 HNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYD 221
           H  WK+L+R+V+  +  RPD HTLLPLP   ++PG+RFREVYYWDSYW++RGLL SKMY+
Sbjct: 122 HALWKDLTRRVAPAVAARPDRHTLLPLPGRVVVPGSRFREVYYWDSYWVVRGLLVSKMYE 181

Query: 222 TAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPAL 281
           TAK IV+NL+ +++++G VLNGAR+YYTNRSQPPLLSSMV DIY+ TGD+ FVR   P+L
Sbjct: 182 TAKDIVLNLVYLVEKYGFVLNGARSYYTNRSQPPLLSSMVLDIYMATGDMAFVRRVFPSL 241

Query: 282 IKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRL 341
           +KEH FW S  H+V V    G  H+LSRY AMWN+PRPES+ +DE+ ASK ++   K++ 
Sbjct: 242 LKEHSFWMSEVHNVAVMDNHGRVHNLSRYQAMWNKPRPESATIDEEFASK-LSTAAKEKF 301

Query: 342 YRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFC 401
           Y ++AS AE+GWDFSSRWMRD TD++TL T+ I+PVDLN FILKME DI+  A+  G+  
Sbjct: 302 YHQVASTAETGWDFSSRWMRDSTDMTTLTTSCIIPVDLNTFILKMEQDIAFFAKLIGEST 361

Query: 402 LAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL-DNGSHKGVHTWDAQNQNKNIYASNFI 461
            +  F EAS  R   I+S+ WN++  QWLDYWL  +G+ +GV+ W + +QN+ I+ASNF+
Sbjct: 362 TSEIFSEASKARHNAIDSVLWNADMEQWLDYWLPTDGNCQGVYQWKSISQNRAIFASNFV 421

Query: 462 PLWIESFYN------DSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQH 521
           PLW+ + ++      D  +  +V++SL+KSGLL  AGIATS  N+G+QWDFPNGWAP+QH
Sbjct: 422 PLWLNAQHSGLEQFVDEAKSVRVMRSLQKSGLLQPAGIATSLSNTGQQWDFPNGWAPLQH 481

Query: 522 MIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQ 581
           +I+EGL RS   EAR LAED+A RW+RTNY AYK TG MHEKYDV  CG  G GGEY PQ
Sbjct: 482 LIVEGLLRSGSGEARELAEDIATRWVRTNYDAYKATGAMHEKYDVVTCGKSGGGGEYKPQ 541

Query: 582 TGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           TGFGWSNGV+L+FL+EFGWP+D+ IDC
Sbjct: 542 TGFGWSNGVILSFLDEFGWPQDKKIDC 563

BLAST of CmoCh02G009180 vs. Swiss-Prot
Match: TREA_HUMAN (Trehalase OS=Homo sapiens GN=TREH PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 3.1e-107
Identity = 207/523 (39.58%), Postives = 301/523 (57.55%), Query Frame = 1

Query: 74  KLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSD 133
           KL  D K +VD+ L        + F  LSR  N S+P E L++F+ E+F + G ++    
Sbjct: 46  KLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWT 105

Query: 134 PLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCII 193
           P D+   PQ FL K+ +A +RAWA ++H  WK L +K+  +++  P+  +L+    P I+
Sbjct: 106 PADWKDSPQ-FLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEHPFIV 165

Query: 194 PGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQP 253
           PG RF E YYWDSYW++ GLL S+M +T KG++ N + ++  +GHV NG R YY  RSQP
Sbjct: 166 PGGRFVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVKTYGHVPNGGRVYYLQRSQP 225

Query: 254 PLLSSMVYDIYL-RTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAM 313
           PLL+ M+ D YL  T D  F++ ++  L  E  FW     +V+V    G N+ L+RYY  
Sbjct: 226 PLLTLMM-DCYLTHTNDTAFLQENIETLALELDFWTKN-RTVSV-SLEGKNYLLNRYYVP 285

Query: 314 WNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWM---RDHTDLSTLA 373
           +  PRPES   D +LA       D++ L+ EL + AESGWDFSSRW+    +   LS + 
Sbjct: 286 YGGPRPESYSKDVELADTLPEG-DREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIR 345

Query: 374 TTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWL 433
           T+ ++PVDLN F+ + E  +SN     G+   A K+      R   +N++ W+ + G W 
Sbjct: 346 TSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWF 405

Query: 434 DYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC-N 493
           DY L+            + +N+  Y SN  PLW   F +  +   K LK L  + +L   
Sbjct: 406 DYDLEK-----------KKKNREFYPSNLTPLWAGCFSDPGVA-DKALKYLEDNRILTYQ 465

Query: 494 AGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKK 553
            GI TS   +G+QWDFPN WAP+Q ++I GLA++ L+ A+ +A  +A  W+RTN+  Y +
Sbjct: 466 YGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQ 525

Query: 554 TGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
              M+EKYDV   G  G GGEY  Q GFGW+NGVVL  L+ +G
Sbjct: 526 KSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYG 551

BLAST of CmoCh02G009180 vs. Swiss-Prot
Match: TREA_MOUSE (Trehalase OS=Mus musculus GN=Treh PE=1 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 9.5e-104
Identity = 199/519 (38.34%), Postives = 291/519 (56.07%), Query Frame = 1

Query: 78  DLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDF 137
           D K +VD+SL    +   + F  L+   N S+P E L+ F+  +F   G ++    P D+
Sbjct: 47  DDKQFVDMSLATSPDEVLQKFSELATVHNHSIPKEQLQEFVQSHFQPVGQELQSWTPEDW 106

Query: 138 VPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGAR 197
              PQ FL K+ +A +R WA E+H  WK L +K+  +++  P+  +L+    P I+PG R
Sbjct: 107 KDSPQ-FLQKISDANLRVWAEELHKIWKKLGKKMKAEVLSYPERSSLIYSKHPFIVPGGR 166

Query: 198 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 257
           F E YYWDSYW++ GLL S+M  T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 167 FVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLT 226

Query: 258 SMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGG-NHSLSRYYAMWNEP 317
            M+      T D+ F++ ++  L  E  FW     + TV    GG ++ L+RYY  +  P
Sbjct: 227 LMMDRYVAHTKDVAFLQENIGTLASELDFWTV---NRTVSVVSGGQSYVLNRYYVPYGGP 286

Query: 318 RPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTD---LSTLATTSI 377
           RPES   D +LA+  V   D++ L+ EL + AESGWDFSSRW+    D   LS++ T+ +
Sbjct: 287 RPESYRKDAELANS-VPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKM 346

Query: 378 LPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL 437
           +P DLN F+ + E  +SN     G+   A K+      R   + ++ W+ +KG W DY L
Sbjct: 347 VPADLNAFLCQAEELMSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKGAWFDYDL 406

Query: 438 DNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC-NAGIA 497
           + G            +N   Y SN  PLW   F + S+   K LK L  S +L    GI 
Sbjct: 407 EKGK-----------KNLEFYPSNLSPLWAGCFSDPSVA-DKALKYLEDSKILTYQYGIP 466

Query: 498 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 557
           TS  N+G+QWDFPN WAP+Q ++I GLA+S     + +A  +A  W++TN+  Y +   M
Sbjct: 467 TSLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNFKVYSQKSAM 526

Query: 558 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
            EKYD+   G  G GGEY  Q GFGW+NG+ L  L+ +G
Sbjct: 527 FEKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYG 548

BLAST of CmoCh02G009180 vs. Swiss-Prot
Match: TREA_RABIT (Trehalase OS=Oryctolagus cuniculus GN=TREH PE=1 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 9.5e-104
Identity = 198/519 (38.15%), Postives = 297/519 (57.23%), Query Frame = 1

Query: 78  DLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDF 137
           D K +VD+ L    +   ++F  L+ + N +VP E L+ F+ E+F + G ++    P D+
Sbjct: 50  DDKQFVDMPLSTAPDQVLQSFAELAATYNNTVPREQLEKFVQEHFQAVGQELESWTPGDW 109

Query: 138 VPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGAR 197
              PQ FL K+ +  +RAWA ++H  WK L +K+  +++ +P+  +L+    P I+PG R
Sbjct: 110 KESPQ-FLQKISDPKLRAWAEQLHLLWKKLGKKIKPEVLSQPERFSLIYSQHPFIVPGGR 169

Query: 198 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 257
           F E YYWDSYW++ GLL S+M +T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 170 FVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVTAYGHIPNGGRVYYLQRSQPPLLT 229

Query: 258 SMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGN-HSLSRYYAMWNEP 317
            M+      TGDL F+R ++  L  E  FW     + T+  + GGN H+L+RY+  +  P
Sbjct: 230 LMMDRYVAHTGDLAFLRENIETLALELDFWAE---NRTISVSSGGNSHTLNRYHVPYGGP 289

Query: 318 RPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWM---RDHTDLSTLATTSI 377
           RPES   D +LA         + L+ EL + AESGWDFSSRW+    +   L ++ T+ +
Sbjct: 290 RPESYSKDTELAHTLPEG-SWETLWAELKAGAESGWDFSSRWLVGSPNPDSLGSIRTSKL 349

Query: 378 LPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL 437
           +PVDLN F+ + E  +S      G+   A K+      R   + ++ W+ +KG W DY L
Sbjct: 350 VPVDLNAFLCQAEELLSGFYSRLGNESQATKYRNLRAQRIAALTALLWDEDKGAWFDYDL 409

Query: 438 DNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNA-GIA 497
           +N           Q +N   Y SN  PLW   F + +I   K L+ L+ S +L +  GI 
Sbjct: 410 EN-----------QKKNHEFYPSNLTPLWAGCFSDPAIA-DKALQYLQDSQILNHRHGIP 469

Query: 498 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 557
           TS  N+G+QWDFPN WAP+Q ++I GLA+S     + +A  +A  W+RTN+  Y +   M
Sbjct: 470 TSLQNTGQQWDFPNAWAPLQDLVIRGLAKSPSARTQEVAFQLAQNWIRTNFDVYSQRSAM 529

Query: 558 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
           +EKYD+      G GGEY  Q GFGW+NGV L  L+ +G
Sbjct: 530 YEKYDISNAQP-GGGGEYEVQEGFGWTNGVALMLLDRYG 550

BLAST of CmoCh02G009180 vs. TrEMBL
Match: A0A0A0KMF2_CUCSA (Trehalase OS=Cucumis sativus GN=Csa_5G149880 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 2.9e-301
Identity = 498/593 (83.98%), Postives = 539/593 (90.89%), Query Frame = 1

Query: 8   LTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVV 67
           L P    SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKFLER+QVV
Sbjct: 8   LPPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKFLERIQVV 67

Query: 68  ALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGT 127
           ALNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS YFDSAGT
Sbjct: 68  ALNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGT 127

Query: 128 DMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPL 187
           D+VYS+P DF P P GFLPKVEN  VRAWA +IHN+WKNLSR+VSDD+IH PD HTLLPL
Sbjct: 128 DLVYSNPADFDPHPDGFLPKVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPL 187

Query: 188 PMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYY 247
           P P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YY
Sbjct: 188 PEPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYY 247

Query: 248 TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLS 307
           TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHS+TV+    GNHSLS
Sbjct: 248 TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSITVQNG-NGNHSLS 307

Query: 308 RYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLST 367
           RYYAMWNEPRPESSL+DEK+ASKFVNNY+K+ LYRE+ASAAESGWDFSSRWMRD TDLST
Sbjct: 308 RYYAMWNEPRPESSLVDEKVASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLST 367

Query: 368 LATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQ 427
           LATTSILPVDLNVFILKMELDISNLARA GD+C A  F EASLVRKKTINSIFWNSEKGQ
Sbjct: 368 LATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQ 427

Query: 428 WLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC 487
           WLDYWLDNGS+KG H+WD +NQN+N+YASNFIPLW+ESFY+DS QMKKVLKSLR SGLLC
Sbjct: 428 WLDYWLDNGSYKGAHSWDVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLC 487

Query: 488 NAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYK 547
           NAGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLRTNYVAYK
Sbjct: 488 NAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYK 547

Query: 548 KTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
            TG+MHEKYDVQKCG FG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 548 HTGFMHEKYDVQKCGGFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of CmoCh02G009180 vs. TrEMBL
Match: M5WD65_PRUPE (Trehalase OS=Prunus persica GN=PRUPE_ppa003514mg PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 5.9e-238
Identity = 383/553 (69.26%), Postives = 462/553 (83.54%), Query Frame = 1

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGS 108
           GPV P   LV F +RLQ  AL +FGK  FD K+YVDLSLKF+L+ T+ AFD L +S+NGS
Sbjct: 16  GPVKPTIPLVAFFDRLQETALATFGKTKFDPKFYVDLSLKFELSKTQTAFDQLPKSANGS 75

Query: 109 VPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLS 168
             V+DLK FI+EYF+ AG DMV ++P DFVP P+GFLPKV+N  VRAWALE+H  WKNLS
Sbjct: 76  ASVKDLKEFIAEYFEDAGEDMVLAEPEDFVPEPEGFLPKVKNPEVRAWALEVHLLWKNLS 135

Query: 169 RKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVIN 228
           RKVSD +  RP+ HTLLPLP  C+IPG+RFREVYYWDSYW+IRGLLASKMYDTAK IV N
Sbjct: 136 RKVSDGVHKRPEFHTLLPLPQQCVIPGSRFREVYYWDSYWVIRGLLASKMYDTAKAIVSN 195

Query: 229 LISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWN 288
           LI +I+E+G+VLNGARAYYTNRSQPPLLS+MV+++Y RTGD+EF R SLPALIKEH FWN
Sbjct: 196 LIYLIEEYGYVLNGARAYYTNRSQPPLLSAMVFEVYKRTGDVEFARKSLPALIKEHEFWN 255

Query: 289 SGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAA 348
           SG H VTV+ +    H+LSRYYAMWN+PRPESS +D++ AS   N Y+KQ  YRE+ASAA
Sbjct: 256 SGIHKVTVQDSQAQKHTLSRYYAMWNKPRPESSTIDKEFASNISNVYEKQHFYREVASAA 315

Query: 349 ESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEA 408
           ESGWDFS+RWMR+H+D +TLATTSILPVDLN FIL+ME DI+ LA+  GD  +A +FL+A
Sbjct: 316 ESGWDFSTRWMRNHSDFTTLATTSILPVDLNAFILRMEHDIALLAKVTGDHNIAERFLKA 375

Query: 409 SLVRKKTINSIFWNSEKGQWLDYWLDNGS-HKGVHTWDAQNQNKNIYASNFIPLWIESFY 468
           S  R + I ++FWN+EKGQWLDYWL N + +    TW+A NQN+N++ASNF+PLWIE F+
Sbjct: 376 SEARHEAIKTVFWNAEKGQWLDYWLGNSTCNAEAQTWEACNQNQNVFASNFVPLWIEPFF 435

Query: 469 NDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEA 528
           +D+  ++KV +SL+ SGLLC+AGIATS   SGEQWDFPNGWAPIQHMI+EGLARS LKEA
Sbjct: 436 SDASLVEKVTRSLQSSGLLCDAGIATSLTKSGEQWDFPNGWAPIQHMIVEGLARSGLKEA 495

Query: 529 RVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFL 588
           +++AED+AVRW+RTNYVAYKKTG MHEKYDV+KCG FG GGEY+PQTGFGWSNGVVLAFL
Sbjct: 496 KLVAEDIAVRWIRTNYVAYKKTGTMHEKYDVEKCGAFGGGGEYIPQTGFGWSNGVVLAFL 555

Query: 589 EEFGWPEDQNIDC 601
           EEFGWP+D+ I+C
Sbjct: 556 EEFGWPQDRRINC 568

BLAST of CmoCh02G009180 vs. TrEMBL
Match: W0D848_CAMSI (Trehalase OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 7.5e-233
Identity = 387/600 (64.50%), Postives = 471/600 (78.50%), Query Frame = 1

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MA F P++ P       +F+  L+   +   + P P     SP  +  GPV+  + L+ F
Sbjct: 1   MANFNPII-PHPFSPFIIFILSLIFTSVTV-STPTP-----SPTCSNSGPVVRKTPLITF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LE LQ+ ALN+ G  +FD K YVDLSLK +L+ T+ AFD L R+ NGSV V D   FI  
Sbjct: 61  LEILQLTALNTLGPKNFDPKLYVDLSLKSNLSITQNAFDDLPRTPNGSVSVTDFNGFIQS 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YF+ AG D+VY++P+DFVP P+GFLP+V N  VRAWALE+H+ WKNLSRKVSD ++ RP+
Sbjct: 121 YFNGAGEDLVYTEPVDFVPEPEGFLPEVVNREVRAWALEVHSLWKNLSRKVSDRVLDRPE 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
            HTLLPL  P +IPG+RFREVYYWDSYW+IRGLLASKMY+TAKGIV NL+S+IDEFGHVL
Sbjct: 181 FHTLLPLAKPVMIPGSRFREVYYWDSYWVIRGLLASKMYETAKGIVTNLVSLIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLS+MVY+IY RTGD++F R SLP L+KEH FWNS  HSV +  A 
Sbjct: 241 NGARAYYTNRSQPPLLSAMVYEIYTRTGDIDFARKSLPVLLKEHKFWNSEIHSVIIHDAE 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           G N +LSRYYAMWN+PRPESS +D + ASK +N+  KQ LYRELAS AESGWDFS+RWMR
Sbjct: 301 GCNRTLSRYYAMWNKPRPESSTIDIETASKLMNDSAKQHLYRELASTAESGWDFSTRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           +  DL+TLATTSI+PVDLN FILKMELDI+ LA   GD  ++  F EA+  RK+ +NS+F
Sbjct: 361 NGYDLTTLATTSIIPVDLNAFILKMELDIAFLANVTGDCTISVHFQEAAQARKQGMNSVF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WN+E GQW DYWL NG+    HTW+A NQN+NI+ASNF+PLWIE F +D   +++V+++L
Sbjct: 421 WNAEMGQWFDYWLSNGTTCKGHTWEASNQNRNIFASNFVPLWIELFNSDCTLVEQVMQNL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGL+   GIATS  NSG+QWDFPNGWAP+QHMI+EGLARS  KEAR +AED+AVRW+R
Sbjct: 481 RSSGLIRAVGIATSLTNSGQQWDFPNGWAPLQHMIVEGLARSGSKEARSMAEDIAVRWIR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKK+G MHEKYDV+KCG+FG GGEY+PQTGFGWSNGVVLAFLEEFGWP+D  IDC
Sbjct: 541 TNYVAYKKSGAMHEKYDVEKCGEFGGGGEYIPQTGFGWSNGVVLAFLEEFGWPQDLKIDC 593

BLAST of CmoCh02G009180 vs. TrEMBL
Match: W9RWI4_9ROSA (Trehalase OS=Morus notabilis GN=L484_007814 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.2e-230
Identity = 387/585 (66.15%), Postives = 464/585 (79.32%), Query Frame = 1

Query: 16  LTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKL 75
           L L LF L  V  L   +P       SP  +  GPV P + L+ FLE +Q  AL SFGK 
Sbjct: 15  LLLLLFSLTQVFSLSQTSPPKC----SPNSS--GPVYPTTPLLTFLENVQETALFSFGKS 74

Query: 76  DFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPL 135
           DFD K YVDLSLK DL++T+RAFD L R SNGSV  EDL+ F+ EYF+ AG D+VY  P 
Sbjct: 75  DFDPKLYVDLSLKLDLSTTDRAFDELHRYSNGSVSAEDLREFLREYFEGAGEDLVYVKPE 134

Query: 136 DFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPG 195
           DFVP P+GFLPKV+N  VR WALE+H+ WKNLSRKVSD+++  P+ HTLLPLP   IIPG
Sbjct: 135 DFVPEPEGFLPKVKNEQVRNWALEVHSLWKNLSRKVSDEVLKNPELHTLLPLPHHVIIPG 194

Query: 196 ARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPL 255
           +RFREVYYWDSYW+IRGLL SKMYDTAK IV NLIS+I+++GHVLNGARAYYTNRSQPPL
Sbjct: 195 SRFREVYYWDSYWVIRGLLTSKMYDTAKAIVTNLISLIEKYGHVLNGARAYYTNRSQPPL 254

Query: 256 LSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNE 315
           LS+MV++IY RTGDLEF R SLP LIKEH FWNSG H VTV+ A G NH+L+RYYAMWN+
Sbjct: 255 LSAMVHEIYKRTGDLEFARKSLPPLIKEHKFWNSGIHKVTVQDAKGLNHTLTRYYAMWNK 314

Query: 316 PRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILP 375
           PRPES  +D++ AS   +  +KQ+ Y E+AS AESGWDFS+RWM + ++ +TLATTSILP
Sbjct: 315 PRPESFTMDKEFASNITSFSEKQQFYHEVASTAESGWDFSTRWMGNPSNFTTLATTSILP 374

Query: 376 VDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDN 435
           VDLN FIL MELDI+ LA+  GD C++++F +AS  RKK + S+FWN++ GQWLDYWL N
Sbjct: 375 VDLNAFILGMELDIALLAKVTGDSCISHRFEKASRARKKAMESVFWNAKTGQWLDYWLSN 434

Query: 436 GSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATST 495
              KGV  W A NQN+  +ASNFIPLWIESF++D+  ++KV +SL++SGL+ +AGIATS 
Sbjct: 435 RKCKGVQKWKAWNQNEKTFASNFIPLWIESFHSDAPLVRKVARSLQRSGLVRDAGIATSL 494

Query: 496 INSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEK 555
            NS +QWDFPNGWAPIQHMIIEGL +S +KEAR+LAE++AVRW+RTNYV YKKTG MHEK
Sbjct: 495 TNSRQQWDFPNGWAPIQHMIIEGLVKSGVKEARLLAEEIAVRWIRTNYVCYKKTGNMHEK 554

Query: 556 YDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           Y+V+KCG FG GGEYVPQTGFGWSNGVVLA LEEFGWPED  IDC
Sbjct: 555 YNVEKCGYFGGGGEYVPQTGFGWSNGVVLALLEEFGWPEDLRIDC 593

BLAST of CmoCh02G009180 vs. TrEMBL
Match: A0A061G5J1_THECC (Trehalase OS=Theobroma cacao GN=TCM_016537 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.2e-230
Identity = 380/587 (64.74%), Postives = 470/587 (80.07%), Query Frame = 1

Query: 18  LFLFFLVVVPLLPPAAPMP-LHEVYSPRLAYR--GPVIPVSNLVKFLERLQVVALNSFGK 77
           LFLF + V  LL    PM  ++   +P    +  GPV+P   L+ FL+R+Q  AL ++GK
Sbjct: 13  LFLFLVSVFFLLLSLTPMSAVNSDSAPSSCSKDSGPVVPTIPLLGFLQRVQETALKTYGK 72

Query: 78  LDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDP 137
             FD K YVDLSLKF+L++T  AFD L +++NGSV V+DL +FI EYF  AG D+VY++P
Sbjct: 73  SSFDPKDYVDLSLKFNLSTTVEAFDKLPKTANGSVSVKDLDAFIGEYFKGAGEDLVYAEP 132

Query: 138 LDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIP 197
           LDFVP P GFLPKVEN  VRAWALE+H  WKNLSRKVS  +   P+ HT+LPLP P +IP
Sbjct: 133 LDFVPEPHGFLPKVENPEVRAWALEVHALWKNLSRKVSTSVHQHPELHTMLPLPGPVVIP 192

Query: 198 GARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPP 257
           G+RFREVYYWDSYW+IRGLLASKMY+TAK IVINLIS++D +GHVLNGARAYYTNRSQPP
Sbjct: 193 GSRFREVYYWDSYWVIRGLLASKMYETAKAIVINLISLLDTYGHVLNGARAYYTNRSQPP 252

Query: 258 LLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWN 317
           LLS+MVY+IY RTGD+E VR +LP L+KE+ FWNS  H V +  A  GNHSL+RYYAMWN
Sbjct: 253 LLSAMVYEIYNRTGDVELVRKALPPLLKEYQFWNSEIHKVIISDAELGNHSLNRYYAMWN 312

Query: 318 EPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSIL 377
           +PRPESS +D+K ASK +N+ +KQ+ YRELAS AESGWDFS+RWMR+ ++ +TLATT IL
Sbjct: 313 KPRPESSTMDKKFASKLLNDCEKQQFYRELASTAESGWDFSTRWMRNPSEFTTLATTKIL 372

Query: 378 PVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLD 437
           PVDLN+FIL+ME+DI+  A+  G+  +A  FL AS  R+K  NS+FWN++ GQWLDYWL+
Sbjct: 373 PVDLNIFILRMEVDIAFFAKVVGENTVAEDFLRASQTRQKAFNSVFWNAKMGQWLDYWLN 432

Query: 438 NGSH-KGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIAT 497
           N +  +   TW+AQNQN+N++ASNF+PLWI+ F +D+  ++KV +SL+ SGLLC AGIAT
Sbjct: 433 NNAACEESQTWEAQNQNENVFASNFVPLWIDLFNSDTPLVEKVTRSLQSSGLLCAAGIAT 492

Query: 498 STINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMH 557
           S  NSG QWDFPNGWAP+QHMI+EGL+RS   EAR +A+D+A RW+RTNYVAYKKTG MH
Sbjct: 493 SLTNSGNQWDFPNGWAPLQHMIVEGLSRSTSTEARSIAKDIAERWIRTNYVAYKKTGAMH 552

Query: 558 EKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           EKYDV+KCG++G GGEY+PQTGFGWSNGVVLAFLEEFGWPEDQ I C
Sbjct: 553 EKYDVEKCGEYGGGGEYIPQTGFGWSNGVVLAFLEEFGWPEDQKIGC 599

BLAST of CmoCh02G009180 vs. TAIR10
Match: AT4G24040.1 (AT4G24040.1 trehalase 1)

HSP 1 Score: 676.0 bits (1743), Expect = 2.1e-194
Identity = 317/555 (57.12%), Postives = 417/555 (75.14%), Query Frame = 1

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDF-DLKYYVDLSLK--FDLNSTERAFDAL-SRS 108
           GPV+  + LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E AFD L S S
Sbjct: 72  GPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTSES 131

Query: 109 SNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYW 168
            +  VPVE L+ F+ EYFD AG D+++ +P+DFV  P GFL  VEN  VR WA E+H  W
Sbjct: 132 HDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHGLW 191

Query: 169 KNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKG 228
           +NLS +VSD +    D HTLLPLP P IIPG+RFREVYYWDSYW+I+GL+ S+M+ TAKG
Sbjct: 192 RNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTAKG 251

Query: 229 IVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH 288
           +V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E VR ++P L+KE+
Sbjct: 252 LVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLKEY 311

Query: 289 MFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYREL 348
            FWNSG H V +R A G +H LSRYYAMWN+PRPESS+ DE+ AS F    +KQR +R++
Sbjct: 312 EFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHRDI 371

Query: 349 ASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYK 408
           A+AAESG DFS+RWMRD  + +T+ATTS++PVDLNVF+LKMELDI+ + + +GD   + +
Sbjct: 372 ATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGSDR 431

Query: 409 FLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIE 468
           F++AS  R+K   ++FWN + GQWLDYWL +   +   TW A+NQN N++ASNF P+WI 
Sbjct: 432 FVKASKAREKAFQTVFWNEKAGQWLDYWLSSSGEES-ETWKAENQNTNVFASNFAPIWIN 491

Query: 469 SFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSEL 528
           S  +D   +KKV+ +L+ SGL+  AGI TS  NSG+QWD PNGWAP Q MI+ GL RS +
Sbjct: 492 SINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRSSV 551

Query: 529 KEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVL 588
           KEA+ +AED+A RW+++NY+ YKK+G +HEK  V + G++G GGEY+PQTGFGWSNGV+L
Sbjct: 552 KEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGVIL 611

Query: 589 AFLEEFGWPEDQNID 600
           AFLEE+GWP   +I+
Sbjct: 612 AFLEEYGWPSHLSIE 625

BLAST of CmoCh02G009180 vs. NCBI nr
Match: gi|659074046|ref|XP_008437392.1| (PREDICTED: probable trehalase [Cucumis melo])

HSP 1 Score: 1046.6 bits (2705), Expect = 1.7e-302
Identity = 504/600 (84.00%), Postives = 543/600 (90.50%), Query Frame = 1

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           M    P L P    SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKF
Sbjct: 1   MPVLHPHLPPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS 
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISN 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YF SAGTD+VYS+P+DF P+P GFLPKVEN  VR WAL+IHN+WKNLSR+VSDD+IH PD
Sbjct: 121 YFHSAGTDLVYSNPVDFNPQPDGFLPKVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
            HTLLPLP P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 THTLLPLPEPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGAR+YYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHSVTV+   
Sbjct: 241 NGARSYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG- 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
            GNHSLSRYYAMWNEPRPESSL+DEKLASKF NNY+K+ LYRE+ASAAESGWDFSSRWMR
Sbjct: 301 NGNHSLSRYYAMWNEPRPESSLVDEKLASKFANNYEKKHLYREIASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           D TDLSTLATTSILPVDLNVFILKMELDISNLARA GD+C A  F EASLVRKKTINSIF
Sbjct: 361 DSTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGS+KG H+W+A+NQN+NIYASNFIPLWIESFY+DS QMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSYKGAHSWNARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLR
Sbjct: 481 RNSGLLCNAGIATSVINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYK TG+MHEKYDVQKCGDFG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 541 TNYVAYKHTGFMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of CmoCh02G009180 vs. NCBI nr
Match: gi|778699376|ref|XP_004143867.2| (PREDICTED: probable trehalase [Cucumis sativus])

HSP 1 Score: 1042.0 bits (2693), Expect = 4.1e-301
Identity = 498/593 (83.98%), Postives = 539/593 (90.89%), Query Frame = 1

Query: 8   LTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVV 67
           L P    SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKFLER+QVV
Sbjct: 8   LPPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKFLERIQVV 67

Query: 68  ALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGT 127
           ALNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS YFDSAGT
Sbjct: 68  ALNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGT 127

Query: 128 DMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPL 187
           D+VYS+P DF P P GFLPKVEN  VRAWA +IHN+WKNLSR+VSDD+IH PD HTLLPL
Sbjct: 128 DLVYSNPADFDPHPDGFLPKVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPL 187

Query: 188 PMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYY 247
           P P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YY
Sbjct: 188 PEPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYY 247

Query: 248 TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLS 307
           TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHS+TV+    GNHSLS
Sbjct: 248 TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSITVQNG-NGNHSLS 307

Query: 308 RYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLST 367
           RYYAMWNEPRPESSL+DEK+ASKFVNNY+K+ LYRE+ASAAESGWDFSSRWMRD TDLST
Sbjct: 308 RYYAMWNEPRPESSLVDEKVASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLST 367

Query: 368 LATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQ 427
           LATTSILPVDLNVFILKMELDISNLARA GD+C A  F EASLVRKKTINSIFWNSEKGQ
Sbjct: 368 LATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQ 427

Query: 428 WLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC 487
           WLDYWLDNGS+KG H+WD +NQN+N+YASNFIPLW+ESFY+DS QMKKVLKSLR SGLLC
Sbjct: 428 WLDYWLDNGSYKGAHSWDVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLC 487

Query: 488 NAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYK 547
           NAGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLRTNYVAYK
Sbjct: 488 NAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYK 547

Query: 548 KTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
            TG+MHEKYDVQKCG FG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 548 HTGFMHEKYDVQKCGGFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of CmoCh02G009180 vs. NCBI nr
Match: gi|595857250|ref|XP_007210503.1| (hypothetical protein PRUPE_ppa003514mg [Prunus persica])

HSP 1 Score: 831.6 bits (2147), Expect = 8.5e-238
Identity = 383/553 (69.26%), Postives = 462/553 (83.54%), Query Frame = 1

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGS 108
           GPV P   LV F +RLQ  AL +FGK  FD K+YVDLSLKF+L+ T+ AFD L +S+NGS
Sbjct: 16  GPVKPTIPLVAFFDRLQETALATFGKTKFDPKFYVDLSLKFELSKTQTAFDQLPKSANGS 75

Query: 109 VPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLS 168
             V+DLK FI+EYF+ AG DMV ++P DFVP P+GFLPKV+N  VRAWALE+H  WKNLS
Sbjct: 76  ASVKDLKEFIAEYFEDAGEDMVLAEPEDFVPEPEGFLPKVKNPEVRAWALEVHLLWKNLS 135

Query: 169 RKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVIN 228
           RKVSD +  RP+ HTLLPLP  C+IPG+RFREVYYWDSYW+IRGLLASKMYDTAK IV N
Sbjct: 136 RKVSDGVHKRPEFHTLLPLPQQCVIPGSRFREVYYWDSYWVIRGLLASKMYDTAKAIVSN 195

Query: 229 LISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWN 288
           LI +I+E+G+VLNGARAYYTNRSQPPLLS+MV+++Y RTGD+EF R SLPALIKEH FWN
Sbjct: 196 LIYLIEEYGYVLNGARAYYTNRSQPPLLSAMVFEVYKRTGDVEFARKSLPALIKEHEFWN 255

Query: 289 SGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAA 348
           SG H VTV+ +    H+LSRYYAMWN+PRPESS +D++ AS   N Y+KQ  YRE+ASAA
Sbjct: 256 SGIHKVTVQDSQAQKHTLSRYYAMWNKPRPESSTIDKEFASNISNVYEKQHFYREVASAA 315

Query: 349 ESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEA 408
           ESGWDFS+RWMR+H+D +TLATTSILPVDLN FIL+ME DI+ LA+  GD  +A +FL+A
Sbjct: 316 ESGWDFSTRWMRNHSDFTTLATTSILPVDLNAFILRMEHDIALLAKVTGDHNIAERFLKA 375

Query: 409 SLVRKKTINSIFWNSEKGQWLDYWLDNGS-HKGVHTWDAQNQNKNIYASNFIPLWIESFY 468
           S  R + I ++FWN+EKGQWLDYWL N + +    TW+A NQN+N++ASNF+PLWIE F+
Sbjct: 376 SEARHEAIKTVFWNAEKGQWLDYWLGNSTCNAEAQTWEACNQNQNVFASNFVPLWIEPFF 435

Query: 469 NDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEA 528
           +D+  ++KV +SL+ SGLLC+AGIATS   SGEQWDFPNGWAPIQHMI+EGLARS LKEA
Sbjct: 436 SDASLVEKVTRSLQSSGLLCDAGIATSLTKSGEQWDFPNGWAPIQHMIVEGLARSGLKEA 495

Query: 529 RVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFL 588
           +++AED+AVRW+RTNYVAYKKTG MHEKYDV+KCG FG GGEY+PQTGFGWSNGVVLAFL
Sbjct: 496 KLVAEDIAVRWIRTNYVAYKKTGTMHEKYDVEKCGAFGGGGEYIPQTGFGWSNGVVLAFL 555

Query: 589 EEFGWPEDQNIDC 601
           EEFGWP+D+ I+C
Sbjct: 556 EEFGWPQDRRINC 568

BLAST of CmoCh02G009180 vs. NCBI nr
Match: gi|645267694|ref|XP_008239192.1| (PREDICTED: probable trehalase isoform X2 [Prunus mume])

HSP 1 Score: 828.2 bits (2138), Expect = 9.4e-237
Identity = 388/588 (65.99%), Postives = 475/588 (80.78%), Query Frame = 1

Query: 13  HCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSF 72
           H   +  LF  +++ LL P       +  S   +  GPV P   LV F +RLQ  AL +F
Sbjct: 9   HSHSSFSLFSSLLLSLLLPTMTFTT-KASSACTSEPGPVKPTIPLVAFFDRLQETALATF 68

Query: 73  GKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYS 132
           GK +FD K+YVDLSLKF+L+ T++AFD L +S+NGS  V+DLK FI+EYF+ AG DMV +
Sbjct: 69  GKTNFDPKFYVDLSLKFELSKTQKAFDQLPKSANGSASVKDLKEFIAEYFEDAGEDMVLA 128

Query: 133 DPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCI 192
           +P DFVP P+GFLPKV+N  VRAWALE+H  WKNLSRKVSD++  RP+ HTLLPLP  C+
Sbjct: 129 EPEDFVPEPEGFLPKVKNPEVRAWALEVHLLWKNLSRKVSDEVHKRPEFHTLLPLPQQCV 188

Query: 193 IPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQ 252
           IPG+RFREVYYWDSYW+IRGLLASKMY+TAK IV NLI +I+E+G+VLNGARAYYTNRSQ
Sbjct: 189 IPGSRFREVYYWDSYWVIRGLLASKMYNTAKAIVSNLIYLIEEYGYVLNGARAYYTNRSQ 248

Query: 253 PPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAM 312
           PPLLS+MV+++Y RTGD+EF R SLPALIKEH FWNSG H VTVR +    H+LSRYYAM
Sbjct: 249 PPLLSAMVFEVYQRTGDVEFARKSLPALIKEHEFWNSGIHKVTVRDSQAQKHTLSRYYAM 308

Query: 313 WNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTS 372
           WN+PRPESS +D++ AS   N  +KQ  YRE+ASAAESGWDFS+RWMR+H+D +TLATTS
Sbjct: 309 WNKPRPESSTIDKEFASNISNVDEKQHFYREVASAAESGWDFSTRWMRNHSDFTTLATTS 368

Query: 373 ILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYW 432
           ILPVDLN FIL+ME DI+ LA+  GD  +A  FL+AS  R + I ++FWN+EKGQWLDYW
Sbjct: 369 ILPVDLNAFILRMEHDIALLAKVTGDHNIAECFLKASEARHEAIKTVFWNAEKGQWLDYW 428

Query: 433 LDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIA 492
           L N +     TW+A NQN+N++ASNF+PLW+E F++D+  ++KV +SL+ SGLLC+AGIA
Sbjct: 429 LGNSTCNEAQTWEACNQNQNVFASNFVPLWVEPFFSDASLVEKVTRSLQSSGLLCDAGIA 488

Query: 493 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 552
           TS   SGEQWDFPNGWAPIQHMI+EGLARS LKEA+++AED+AVRW+RTNYVAYKKTG M
Sbjct: 489 TSLTKSGEQWDFPNGWAPIQHMIVEGLARSGLKEAKLVAEDIAVRWIRTNYVAYKKTGTM 548

Query: 553 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           HEKYDV+KCG FG GGEY+PQTGFGWSNGVVLAFLEEFGWP+D+ I+C
Sbjct: 549 HEKYDVEKCGAFGGGGEYIPQTGFGWSNGVVLAFLEEFGWPQDRRINC 595

BLAST of CmoCh02G009180 vs. NCBI nr
Match: gi|645267691|ref|XP_008239191.1| (PREDICTED: probable trehalase isoform X1 [Prunus mume])

HSP 1 Score: 824.3 bits (2128), Expect = 1.4e-235
Identity = 388/589 (65.87%), Postives = 476/589 (80.81%), Query Frame = 1

Query: 13  HCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSF 72
           H   +  LF  +++ LL P       +  S   +  GPV P   LV F +RLQ  AL +F
Sbjct: 9   HSHSSFSLFSSLLLSLLLPTMTFTT-KASSACTSEPGPVKPTIPLVAFFDRLQETALATF 68

Query: 73  GKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYS 132
           GK +FD K+YVDLSLKF+L+ T++AFD L +S+NGS  V+DLK FI+EYF+ AG DMV +
Sbjct: 69  GKTNFDPKFYVDLSLKFELSKTQKAFDQLPKSANGSASVKDLKEFIAEYFEDAGEDMVLA 128

Query: 133 DPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCI 192
           +P DFVP P+GFLPKV+N  VRAWALE+H  WKNLSRKVSD++  RP+ HTLLPLP  C+
Sbjct: 129 EPEDFVPEPEGFLPKVKNPEVRAWALEVHLLWKNLSRKVSDEVHKRPEFHTLLPLPQQCV 188

Query: 193 IPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQ 252
           IPG+RFREVYYWDSYW+IRGLLASKMY+TAK IV NLI +I+E+G+VLNGARAYYTNRSQ
Sbjct: 189 IPGSRFREVYYWDSYWVIRGLLASKMYNTAKAIVSNLIYLIEEYGYVLNGARAYYTNRSQ 248

Query: 253 PPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAM 312
           PPLLS+MV+++Y RTGD+EF R SLPALIKEH FWNSG H VTVR +    H+LSRYYAM
Sbjct: 249 PPLLSAMVFEVYQRTGDVEFARKSLPALIKEHEFWNSGIHKVTVRDSQAQKHTLSRYYAM 308

Query: 313 WNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTS 372
           WN+PRPESS +D++ AS   N  +KQ  YRE+ASAAESGWDFS+RWMR+H+D +TLATTS
Sbjct: 309 WNKPRPESSTIDKEFASNISNVDEKQHFYREVASAAESGWDFSTRWMRNHSDFTTLATTS 368

Query: 373 ILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYW 432
           ILPVDLN FIL+ME DI+ LA+  GD  +A  FL+AS  R + I ++FWN+EKGQWLDYW
Sbjct: 369 ILPVDLNAFILRMEHDIALLAKVTGDHNIAECFLKASEARHEAIKTVFWNAEKGQWLDYW 428

Query: 433 LDNGS-HKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGI 492
           L N + +    TW+A NQN+N++ASNF+PLW+E F++D+  ++KV +SL+ SGLLC+AGI
Sbjct: 429 LGNSTCNVEAQTWEACNQNQNVFASNFVPLWVEPFFSDASLVEKVTRSLQSSGLLCDAGI 488

Query: 493 ATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGY 552
           ATS   SGEQWDFPNGWAPIQHMI+EGLARS LKEA+++AED+AVRW+RTNYVAYKKTG 
Sbjct: 489 ATSLTKSGEQWDFPNGWAPIQHMIVEGLARSGLKEAKLVAEDIAVRWIRTNYVAYKKTGT 548

Query: 553 MHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           MHEKYDV+KCG FG GGEY+PQTGFGWSNGVVLAFLEEFGWP+D+ I+C
Sbjct: 549 MHEKYDVEKCGAFGGGGEYIPQTGFGWSNGVVLAFLEEFGWPQDRRINC 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TRE1_ARATH3.7e-19357.12Trehalase OS=Arabidopsis thaliana GN=TRE1 PE=2 SV=1[more]
TRE_ORYSJ3.1e-18755.56Probable trehalase OS=Oryza sativa subsp. japonica GN=Os10g0521000 PE=2 SV=1[more]
TREA_HUMAN3.1e-10739.58Trehalase OS=Homo sapiens GN=TREH PE=1 SV=2[more]
TREA_MOUSE9.5e-10438.34Trehalase OS=Mus musculus GN=Treh PE=1 SV=1[more]
TREA_RABIT9.5e-10438.15Trehalase OS=Oryctolagus cuniculus GN=TREH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMF2_CUCSA2.9e-30183.98Trehalase OS=Cucumis sativus GN=Csa_5G149880 PE=3 SV=1[more]
M5WD65_PRUPE5.9e-23869.26Trehalase OS=Prunus persica GN=PRUPE_ppa003514mg PE=3 SV=1[more]
W0D848_CAMSI7.5e-23364.50Trehalase OS=Camellia sinensis PE=2 SV=1[more]
W9RWI4_9ROSA1.2e-23066.15Trehalase OS=Morus notabilis GN=L484_007814 PE=3 SV=1[more]
A0A061G5J1_THECC1.2e-23064.74Trehalase OS=Theobroma cacao GN=TCM_016537 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24040.12.1e-19457.12 trehalase 1[more]
Match NameE-valueIdentityDescription
gi|659074046|ref|XP_008437392.1|1.7e-30284.00PREDICTED: probable trehalase [Cucumis melo][more]
gi|778699376|ref|XP_004143867.2|4.1e-30183.98PREDICTED: probable trehalase [Cucumis sativus][more]
gi|595857250|ref|XP_007210503.1|8.5e-23869.26hypothetical protein PRUPE_ppa003514mg [Prunus persica][more]
gi|645267694|ref|XP_008239192.1|9.4e-23765.99PREDICTED: probable trehalase isoform X2 [Prunus mume][more]
gi|645267691|ref|XP_008239191.1|1.4e-23565.87PREDICTED: probable trehalase isoform X1 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001661Glyco_hydro_37
IPR0089286-hairpin_glycosidase_sf
IPR018232Glyco_hydro_37_CS
Vocabulary: Molecular Function
TermDefinition
GO:0004555alpha,alpha-trehalase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0005991trehalose metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005993 trehalose catabolic process
biological_process GO:0005991 trehalose metabolic process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0004555 alpha,alpha-trehalase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G009180.1CmoCh02G009180.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001661Glycoside hydrolase, family 37PRINTSPR00744GLHYDRLASE37coord: 416..432
score: 4.1E-43coord: 574..587
score: 4.1E-43coord: 373..390
score: 4.1E-43coord: 236..254
score: 4.1E-43coord: 341..358
score: 4.1E-43coord: 306..323
score: 4.1E-43coord: 494..510
score: 4.1
IPR001661Glycoside hydrolase, family 37PANTHERPTHR23403TREHALASEcoord: 35..600
score: 3.8E
IPR001661Glycoside hydrolase, family 37PFAMPF01204Trehalasecoord: 75..591
score: 1.8E
IPR008928Six-hairpin glycosidase-likeunknownSSF48208Six-hairpin glycosidasescoord: 72..593
score: 2.52E
IPR018232Glycoside hydrolase, family 37, conserved sitePROSITEPS00928TREHALASE_2coord: 501..510
scor
NoneNo IPR availablePANTHERPTHR23403:SF1TREHALASEcoord: 35..600
score: 3.8E