Lsi08G012150 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G012150
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	chr08 : 20656977 .. 20657414 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCAAATTTCTTTCCTTATCAACCATTCTGATCTTCTCTCTCCTCCTCTGCTCTGCCCTGGCCGCCCCATGCGACCAACAACCCAAAACCAAGCCGGCGCCGGCCGTGCCAAAAAGAAAACCCGCCATCCCCAAAATAATCCGGCCGCCATCGGCATCTTCACCGGCAGTACAATCGAGTTACTGCCCAAAAGACACCGTGAAGTTCGGTGTTTGTACCGACATTCTAGGAATTGGGAGTGTAATTGGAAGCCCTGTTTCGAAAAAATGCTGTTCTCTGCTTAGTGGGCTGACAAATGTTGAAGCTGCCGCTTGTCTTTGCACTGCCATTAAAGCAAATGTTCTTGGAATTGACTTGGATATCCCTGTTTCACTCAGTTTGCTCATCAGCTCTTGCAAAAAATCTGTTCCCACTGGCTACCAGTGTAAATGA

mRNA sequence

ATGGCTTCCAAATTTCTTTCCTTATCAACCATTCTGATCTTCTCTCTCCTCCTCTGCTCTGCCCTGGCCGCCCCATGCGACCAACAACCCAAAACCAAGCCGGCGCCGGCCGTGCCAAAAAGAAAACCCGCCATCCCCAAAATAATCCGGCCGCCATCGGCATCTTCACCGGCAGTACAATCGAGTTACTGCCCAAAAGACACCGTGAAGTTCGGTGTTTGTACCGACATTCTAGGAATTGGGAGTGTAATTGGAAGCCCTGTTTCGAAAAAATGCTGTTCTCTGCTTAGTGGGCTGACAAATGTTGAAGCTGCCGCTTGTCTTTGCACTGCCATTAAAGCAAATGTTCTTGGAATTGACTTGGATATCCCTGTTTCACTCAGTTTGCTCATCAGCTCTTGCAAAAAATCTGTTCCCACTGGCTACCAGTGTAAATGA

Coding sequence (CDS)

ATGGCTTCCAAATTTCTTTCCTTATCAACCATTCTGATCTTCTCTCTCCTCCTCTGCTCTGCCCTGGCCGCCCCATGCGACCAACAACCCAAAACCAAGCCGGCGCCGGCCGTGCCAAAAAGAAAACCCGCCATCCCCAAAATAATCCGGCCGCCATCGGCATCTTCACCGGCAGTACAATCGAGTTACTGCCCAAAAGACACCGTGAAGTTCGGTGTTTGTACCGACATTCTAGGAATTGGGAGTGTAATTGGAAGCCCTGTTTCGAAAAAATGCTGTTCTCTGCTTAGTGGGCTGACAAATGTTGAAGCTGCCGCTTGTCTTTGCACTGCCATTAAAGCAAATGTTCTTGGAATTGACTTGGATATCCCTGTTTCACTCAGTTTGCTCATCAGCTCTTGCAAAAAATCTGTTCCCACTGGCTACCAGTGTAAATGA

Protein sequence

MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQSSYCPKDTVKFGVCTDILGIGSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQCK

Homology

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BLAST of Lsi08G012150 vs. Swiss-Prot
Match: 14KD_DAUCA (14 kDa proline-rich protein DC2.15 OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 7.5e-23
Identity = 62/145 (42.76%), Postives = 87/145 (60.00%), Query Frame = 1


Query: 2   ASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQS 61
           AS  L  +  ++F  L+ S    P   +PK KP P     KP            +P   +
Sbjct: 7   ASVALFFTLNILFFALVSSTEKCPDPYKPKPKPTP-----KPT----------PTPYPSA 66

Query: 62  SYCPKDTVKFGVCTDILGI--GSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 121
             CP+D +K GVC D+L +    VIGSP +  CCSLL GL N+EAA CLCTAIKAN+LG 
Sbjct: 67  GKCPRDALKLGVCADVLNLVHNVVIGSPPTLPCCSLLEGLVNLEAAVCLCTAIKANILGK 126

Query: 122 DLDIPVSLSLLISSCKKSVPTGYQC 145
           +L++P++LSL++++C K VP G++C
Sbjct: 127 NLNLPIALSLVLNNCGKQVPNGFEC 136

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BLAST of Lsi08G012150 vs. Swiss-Prot
Match: ERLI1_ARATH (Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana GN=EARLI1 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.2e-22
Identity = 69/169 (40.83%), Postives = 99/169 (58.58%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSL-----LLCSALAAPCDQQPKTKPAPAVPKRKPAI---PKIIRPP 60
           MASK  S S  L F+L      L +A    C+  PK KP P+ PK KP     PK +  P
Sbjct: 1   MASKN-SASIALFFALNIIFFTLTAATDCGCNPSPKHKPVPS-PKPKPVPSPKPKPVPSP 60

Query: 61  SASSPAV---------------QSSYCPKDTVKFGVCTDILG--IGSVIGSPVSKKCCSL 120
           S  SP+V                S  CP D ++ GVC ++L   +   +G P ++ CCSL
Sbjct: 61  SVPSPSVPSPNPRPVTPPRTPGSSGNCPIDALRLGVCANVLSSLLNIQLGQPSAQPCCSL 120

Query: 121 LSGLTNVEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           + GL +++AA CLCTA++ANVLGI+L++P+SLS+L++ C + VP+G+QC
Sbjct: 121 IQGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKVPSGFQC 167

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BLAST of Lsi08G012150 vs. Swiss-Prot
Match: ERLL1_ARATH (pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana GN=AZI1 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 6.4e-22
Identity = 67/164 (40.85%), Postives = 96/164 (58.54%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSL-----LLCSALAAPCDQQPKTKPAPAVPKRKPAI---------- 60
           MASK  S S  L F+L      L  A    C   PK KP P+ PK KP            
Sbjct: 1   MASKN-SASLALFFALNILFFTLTVATNCNCKPSPKPKPVPS-PKPKPVQCPPPPRPSVP 60

Query: 61  ---PKIIRPPSASSPAVQSSYCPKDTVKFGVCTDILG--IGSVIGSPVSKKCCSLLSGLT 120
              P+ + PP   +P    + CP D +K GVC ++L   +   +G P S++CCSL+ GL 
Sbjct: 61  SPNPRPVTPPR--TPGSSGNSCPIDALKLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLV 120

Query: 121 NVEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           +V+AA CLCTA++ANVLGI+L++P+SLS+L++ C + +P+G+QC
Sbjct: 121 DVDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKLPSGFQC 160

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BLAST of Lsi08G012150 vs. Swiss-Prot
Match: LBP65_ARATH (Putative lipid-binding protein At4g00165 OS=Arabidopsis thaliana GN=At4g00165 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.6e-20
Identity = 51/96 (53.12%), Postives = 72/96 (75.00%), Query Frame = 1


Query: 52  PSASSPAVQSSY-CPKDTVKFGVCTDILGIGS-VIGSPVSKKCCSLLSGLTNVEAAACLC 111
           P    PA Q++  CP+DT+KFGVC   LG+ S VIG+P S++CCSL+ GL + EAA CLC
Sbjct: 33  PCPPPPAKQATTKCPRDTLKFGVCGSWLGLVSEVIGTPPSQECCSLIKGLADFEAAVCLC 92

Query: 112 TAIKANVLGI-DLDIPVSLSLLISSCKKSVPTGYQC 145
           TA+K ++LG+  + IPV+L+LL++SC K+VP G+ C
Sbjct: 93  TALKTSILGVAPVKIPVALTLLLNSCGKNVPQGFVC 128

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BLAST of Lsi08G012150 vs. Swiss-Prot
Match: ERLL3_ARATH (pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana GN=At4g12500 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-19
Identity = 57/160 (35.62%), Postives = 89/160 (55.62%), Query Frame = 1


Query: 11  ILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRP---------PSASSPAV-- 70
           IL F+L   +        +P+T P+P VP  K   P I  P         PS  +P+V  
Sbjct: 17  ILFFTLTTATDCRCNLSPKPRTVPSPKVPSPKYPSPSIPSPSVPTPSVPTPSVPTPSVPS 76

Query: 71  -------------QSSYCPKDTVKFGVCTDILG--IGSVIGSPVSKKCCSLLSGLTNVEA 130
                         S  CP D ++ GVC ++L   +   +G P ++ CCSL+ GL +++A
Sbjct: 77  PNPTPVTPPRTPGSSGNCPIDALRLGVCANVLSGLLNVQLGQPSAQPCCSLIQGLVDLDA 136

Query: 131 AACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           A CLCTA++ANVLGI+L++P+SLS+L++ C + +P+ +QC
Sbjct: 137 AICLCTALRANVLGINLNVPISLSVLLNVCNRRLPSDFQC 176

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BLAST of Lsi08G012150 vs. TrEMBL
Match: A0A0A0LPN2_CUCSA (Proline-rich protein DC2.15 OS=Cucumis sativus GN=Csa_2G381890 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.5e-57
Identity = 122/146 (83.56%), Postives = 135/146 (92.47%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MASK LSL TIL+ SLLLCSALA PCDQ PKTKPAP+VPK+KPAIPKIIRPP+ASSPAVQ
Sbjct: 1   MASKSLSLPTILLLSLLLCSALATPCDQ-PKTKPAPSVPKKKPAIPKIIRPPAASSPAVQ 60

Query: 61  SSYCPKDTVKFGVCTDILGIGS-VIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 120
           SSYCPKDT+K GVC DILGIGS VIGSPVS  CC+LLSGLT+VEAAACLCTAIKANVLGI
Sbjct: 61  SSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGI 120

Query: 121 DLDIPVSLSLLISSCKKSVPTGYQCK 146
           +L+IPVS+SLLISSC+K++P GYQCK
Sbjct: 121 NLNIPVSISLLISSCQKTLPDGYQCK 145

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BLAST of Lsi08G012150 vs. TrEMBL
Match: G7I725_MEDTR (Protease inhibitor/seed storage/LTP family protein OS=Medicago truncatula GN=MTR_1g012710 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.1e-28
Identity = 78/155 (50.32%), Postives = 104/155 (67.10%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQ---------QPKTKPAPAVPKRKPAIPKIIRP 60
           MAS  LS + I++FSLL  S  +  C           +PK KP+P  P   P  P     
Sbjct: 1   MASNKLS-ALIILFSLLAYSTFSHACGSCKPTPSPKPKPKPKPSPPPPSSTPCPPT---- 60

Query: 61  PSASSPAVQSSY-CPKDTVKFGVCTDILGIGSVI-GSPVSKKCCSLLSGLTNVEAAACLC 120
           PS + P   +S  CP DT+K GVC D+LG+ +VI GSP S KCC+LL GL +++AA CLC
Sbjct: 61  PSTTPPTPSTSQKCPSDTLKLGVCADVLGLVNVIVGSPASSKCCALLQGLVDLDAAICLC 120

Query: 121 TAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           TAIKANVLGI+L++P++LSLL+S+C+KSVP+G+QC
Sbjct: 121 TAIKANVLGINLNVPITLSLLLSACEKSVPSGFQC 150

[Close]

BLAST of Lsi08G012150 vs. TrEMBL
Match: A0A061GJ47_THECC (Bimodular protein OS=Theobroma cacao GN=TCM_036975 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 5.4e-28
Identity = 78/146 (53.42%), Postives = 102/146 (69.86%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MASK +  +  L+FSLLL S   + C+   K KP P  P           PPS + P   
Sbjct: 1   MASKQIC-ALFLVFSLLLHSTFTSACNSC-KPKPTPTPPP----------PPSPACPPPP 60

Query: 61  SSYCPKDTVKFGVCTDILG-IGSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 120
           SS CPKDT+K GVC D+LG +  V+GSP S KCC+LLSGL ++EAA CLCTAIKA+VLGI
Sbjct: 61  SS-CPKDTLKLGVCADLLGLVNIVVGSPPSSKCCALLSGLADLEAALCLCTAIKASVLGI 120

Query: 121 DLDIPVSLSLLISSCKKSVPTGYQCK 146
           +L+IPVSLSL++S+C+K+VP G++C+
Sbjct: 121 NLNIPVSLSLILSACQKNVPAGFKCE 133

[Close]

BLAST of Lsi08G012150 vs. TrEMBL
Match: A0A151TT59_CAJCA (14 kDa proline-rich protein DC2.15 OS=Cajanus cajan GN=KK1_009402 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 7.1e-28
Identity = 78/163 (47.85%), Postives = 101/163 (61.96%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALA-APCDQQPKT------------KPAPAVPKRKPAIPK 60
           MAS    ++TIL+ S+L  S    A C   PK             KP P  PK  P  PK
Sbjct: 1   MASNTKLIATILLLSILSYSTFTGANCPPSPKPTPPSPKPTPPSPKPTPPTPKPTPPSPK 60

Query: 61  IIRP-----PSASSPAVQSSYCPKDTVKFGVCTDILG-IGSVIGSPVSKKCCSLLSGLTN 120
              P     PS S      S CPKDT+K G C D+LG +  +IG+P S KCC+L+ GL +
Sbjct: 61  PTPPSPKPTPSPSPSPPSQSKCPKDTLKLGACADLLGLVNIIIGTPPSSKCCALIKGLAD 120

Query: 121 VEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           +EAA CLCTAIKANVLGI+L++PV+LSL++S+C+K+VPTG+QC
Sbjct: 121 LEAALCLCTAIKANVLGINLNVPVTLSLILSACQKTVPTGFQC 163

[Close]

BLAST of Lsi08G012150 vs. TrEMBL
Match: G7I723_MEDTR (Lipid transfer protein OS=Medicago truncatula GN=MTR_1g012690 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 9.2e-28
Identity = 78/157 (49.68%), Postives = 100/157 (63.69%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MAS  LS + I++FSLL  S  +  C      KP P  P      PK   PP AS+P   
Sbjct: 1   MASNKLS-ALIILFSLLSYSTFSHACGS---CKPTPTSPPPPSTTPKASPPPKASTPPPS 60

Query: 61  ------------SSYCPKDTVKFGVCTDILGIGSVI-GSPVSKKCCSLLSGLTNVEAAAC 120
                       S  CP DT+K GVC D+LG+ +VI GSP S KCC+L+ GL +++AA C
Sbjct: 61  TTPKSSPPTPSTSQKCPSDTLKLGVCADVLGLVNVIVGSPASSKCCTLIQGLADLDAAVC 120

Query: 121 LCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           LCTAIKANVLGI+L++PV+LSLL+S+C+KSVP G+QC
Sbjct: 121 LCTAIKANVLGINLNVPVTLSLLLSACEKSVPNGFQC 153

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BLAST of Lsi08G012150 vs. TAIR10
Match: AT2G45180.1 (AT2G45180.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 113.6 bits (283), Expect = 1.0e-25
Identity = 68/145 (46.90%), Postives = 90/145 (62.07%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MASK L+++ +LI   LL           P T   P  PK     PK        +PAV+
Sbjct: 1   MASKALAVTALLITLNLLFFTFVTSTKCPPTTPKPPKTPKS----PK-------KAPAVK 60

Query: 61  SSYCPKDTVKFGVCTDILG-IGSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 120
            + CP DT+K GVC D+LG +  V+GSP    CC+LL GL N+EAA CLCTA+KANVLGI
Sbjct: 61  PT-CPTDTLKLGVCADLLGLVNVVVGSPPKTPCCTLLQGLANLEAAVCLCTALKANVLGI 120

Query: 121 DLDIPVSLSLLISSCKKSVPTGYQC 145
           +L++P+ L+LL++ C K VP G+QC
Sbjct: 121 NLNVPIDLTLLLNYCGKKVPHGFQC 133

[Close]

BLAST of Lsi08G012150 vs. TAIR10
Match: AT1G62510.1 (AT1G62510.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 110.9 bits (276), Expect = 6.5e-25
Identity = 63/142 (44.37%), Postives = 91/142 (64.08%), Query Frame = 1


Query: 5   FLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQSSYC 64
           FL L+ +   ++  C     P   +PK KP+P+ PK KP       P  + SPA+    C
Sbjct: 12  FLILNFLFFTTISACGNCGCP-SPKPKHKPSPS-PKPKPNPKPKPTPHPSPSPAIAK--C 71

Query: 65  PKDTVKFGVCTDILG--IGSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGIDLD 124
           P+D +K GVC ++L   +   +G P  + CC+L+ GL ++EAAACLCTA+KAN+LGI+L+
Sbjct: 72  PRDALKLGVCANVLNGLLNVTLGKPPVEPCCTLIQGLADLEAAACLCTALKANILGINLN 131

Query: 125 IPVSLSLLISSCKKSVPTGYQC 145
           IP+SLSLL++ C K VP G+QC
Sbjct: 132 IPLSLSLLLNVCSKKVPRGFQC 149

[Close]

BLAST of Lsi08G012150 vs. TAIR10
Match: AT1G12090.1 (AT1G12090.1 extensin-like protein)

HSP 1 Score: 109.0 bits (271), Expect = 2.5e-24
Identity = 63/143 (44.06%), Postives = 88/143 (61.54%), Query Frame = 1


Query: 5   FLSLSTILIFSLLLCSALAAPCDQQ-PKTKPAPAVPKRKPAIPKIIRPPSASSPAVQSSY 64
           FL+L+ +   ++  C +   PC    P  KP P  PK  P            SP+  SS 
Sbjct: 9   FLALNLLFFTTISACGS-CTPCGGGCPSPKPKPT-PKPTP------------SPSSGSSK 68

Query: 65  CPKDTVKFGVCTDILG--IGSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGIDL 124
           CPKDT+K GVC ++L   +   +G P  + CCSL+ GL +VEAA CLCTA+KAN+LGI+L
Sbjct: 69  CPKDTLKLGVCANVLNGLLDLTLGKPPVEPCCSLIQGLADVEAAVCLCTALKANILGINL 128

Query: 125 DIPVSLSLLISSCKKSVPTGYQC 145
           ++P+SLSLL++ C K +P G+QC
Sbjct: 129 NLPISLSLLLNVCSKQLPPGFQC 137

[Close]

BLAST of Lsi08G012150 vs. TAIR10
Match: AT4G12480.1 (AT4G12480.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 106.7 bits (265), Expect = 1.2e-23
Identity = 69/169 (40.83%), Postives = 99/169 (58.58%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSL-----LLCSALAAPCDQQPKTKPAPAVPKRKPAI---PKIIRPP 60
           MASK  S S  L F+L      L +A    C+  PK KP P+ PK KP     PK +  P
Sbjct: 1   MASKN-SASIALFFALNIIFFTLTAATDCGCNPSPKHKPVPS-PKPKPVPSPKPKPVPSP 60

Query: 61  SASSPAV---------------QSSYCPKDTVKFGVCTDILG--IGSVIGSPVSKKCCSL 120
           S  SP+V                S  CP D ++ GVC ++L   +   +G P ++ CCSL
Sbjct: 61  SVPSPSVPSPNPRPVTPPRTPGSSGNCPIDALRLGVCANVLSSLLNIQLGQPSAQPCCSL 120

Query: 121 LSGLTNVEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           + GL +++AA CLCTA++ANVLGI+L++P+SLS+L++ C + VP+G+QC
Sbjct: 121 IQGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKVPSGFQC 167

[Close]

BLAST of Lsi08G012150 vs. TAIR10
Match: AT4G12470.1 (AT4G12470.1 azelaic acid induced 1)

HSP 1 Score: 105.1 bits (261), Expect = 3.6e-23
Identity = 67/164 (40.85%), Postives = 96/164 (58.54%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSL-----LLCSALAAPCDQQPKTKPAPAVPKRKPAI---------- 60
           MASK  S S  L F+L      L  A    C   PK KP P+ PK KP            
Sbjct: 1   MASKN-SASLALFFALNILFFTLTVATNCNCKPSPKPKPVPS-PKPKPVQCPPPPRPSVP 60

Query: 61  ---PKIIRPPSASSPAVQSSYCPKDTVKFGVCTDILG--IGSVIGSPVSKKCCSLLSGLT 120
              P+ + PP   +P    + CP D +K GVC ++L   +   +G P S++CCSL+ GL 
Sbjct: 61  SPNPRPVTPPR--TPGSSGNSCPIDALKLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLV 120

Query: 121 NVEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           +V+AA CLCTA++ANVLGI+L++P+SLS+L++ C + +P+G+QC
Sbjct: 121 DVDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKLPSGFQC 160

[Close]

BLAST of Lsi08G012150 vs. NCBI nr
Match: gi|659088926|ref|XP_008445235.1| (PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis melo])

HSP 1 Score: 233.8 bits (595), Expect = 1.9e-58
Identity = 124/146 (84.93%), Postives = 136/146 (93.15%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MASK LSL TIL+ SLLLCSALA PCDQ PKTKPAP+VPK+KPAIPKIIRPP+ASSPAVQ
Sbjct: 1   MASKSLSLPTILLLSLLLCSALATPCDQ-PKTKPAPSVPKKKPAIPKIIRPPAASSPAVQ 60

Query: 61  SSYCPKDTVKFGVCTDILGIGSV-IGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 120
           SSYCPKDT+K GVC DILGIGSV IGSPVS +CC+LLSGLT+VEAAACLCTAIKANVLGI
Sbjct: 61  SSYCPKDTLKLGVCADILGIGSVVIGSPVSNECCALLSGLTDVEAAACLCTAIKANVLGI 120

Query: 121 DLDIPVSLSLLISSCKKSVPTGYQCK 146
           +LDIPVSLSLLISSC+K++P GYQCK
Sbjct: 121 NLDIPVSLSLLISSCQKTLPAGYQCK 145

[Close]

BLAST of Lsi08G012150 vs. NCBI nr
Match: gi|449441902|ref|XP_004138721.1| (PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis sativus])

HSP 1 Score: 230.3 bits (586), Expect = 2.1e-57
Identity = 122/146 (83.56%), Postives = 135/146 (92.47%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MASK LSL TIL+ SLLLCSALA PCDQ PKTKPAP+VPK+KPAIPKIIRPP+ASSPAVQ
Sbjct: 1   MASKSLSLPTILLLSLLLCSALATPCDQ-PKTKPAPSVPKKKPAIPKIIRPPAASSPAVQ 60

Query: 61  SSYCPKDTVKFGVCTDILGIGS-VIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 120
           SSYCPKDT+K GVC DILGIGS VIGSPVS  CC+LLSGLT+VEAAACLCTAIKANVLGI
Sbjct: 61  SSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGI 120

Query: 121 DLDIPVSLSLLISSCKKSVPTGYQCK 146
           +L+IPVS+SLLISSC+K++P GYQCK
Sbjct: 121 NLNIPVSISLLISSCQKTLPDGYQCK 145

[Close]

BLAST of Lsi08G012150 vs. NCBI nr
Match: gi|357437019|ref|XP_003588785.1| (protease inhibitor/seed storage/LTP family protein [Medicago truncatula])

HSP 1 Score: 132.5 bits (332), Expect = 6.0e-28
Identity = 78/155 (50.32%), Postives = 104/155 (67.10%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQ---------QPKTKPAPAVPKRKPAIPKIIRP 60
           MAS  LS + I++FSLL  S  +  C           +PK KP+P  P   P  P     
Sbjct: 1   MASNKLS-ALIILFSLLAYSTFSHACGSCKPTPSPKPKPKPKPSPPPPSSTPCPPT---- 60

Query: 61  PSASSPAVQSSY-CPKDTVKFGVCTDILGIGSVI-GSPVSKKCCSLLSGLTNVEAAACLC 120
           PS + P   +S  CP DT+K GVC D+LG+ +VI GSP S KCC+LL GL +++AA CLC
Sbjct: 61  PSTTPPTPSTSQKCPSDTLKLGVCADVLGLVNVIVGSPASSKCCALLQGLVDLDAAICLC 120

Query: 121 TAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           TAIKANVLGI+L++P++LSLL+S+C+KSVP+G+QC
Sbjct: 121 TAIKANVLGINLNVPITLSLLLSACEKSVPSGFQC 150

[Close]

BLAST of Lsi08G012150 vs. NCBI nr
Match: gi|590572334|ref|XP_007011824.1| (Bimodular protein [Theobroma cacao])

HSP 1 Score: 132.1 bits (331), Expect = 7.8e-28
Identity = 78/146 (53.42%), Postives = 102/146 (69.86%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALAAPCDQQPKTKPAPAVPKRKPAIPKIIRPPSASSPAVQ 60
           MASK +  +  L+FSLLL S   + C+   K KP P  P           PPS + P   
Sbjct: 1   MASKQIC-ALFLVFSLLLHSTFTSACNSC-KPKPTPTPPP----------PPSPACPPPP 60

Query: 61  SSYCPKDTVKFGVCTDILG-IGSVIGSPVSKKCCSLLSGLTNVEAAACLCTAIKANVLGI 120
           SS CPKDT+K GVC D+LG +  V+GSP S KCC+LLSGL ++EAA CLCTAIKA+VLGI
Sbjct: 61  SS-CPKDTLKLGVCADLLGLVNIVVGSPPSSKCCALLSGLADLEAALCLCTAIKASVLGI 120

Query: 121 DLDIPVSLSLLISSCKKSVPTGYQCK 146
           +L+IPVSLSL++S+C+K+VP G++C+
Sbjct: 121 NLNIPVSLSLILSACQKNVPAGFKCE 133

[Close]

BLAST of Lsi08G012150 vs. NCBI nr
Match: gi|1012359007|gb|KYP70191.1| (14 kDa proline-rich protein DC2.15 [Cajanus cajan])

HSP 1 Score: 131.7 bits (330), Expect = 1.0e-27
Identity = 78/163 (47.85%), Postives = 101/163 (61.96%), Query Frame = 1


Query: 1   MASKFLSLSTILIFSLLLCSALA-APCDQQPKT------------KPAPAVPKRKPAIPK 60
           MAS    ++TIL+ S+L  S    A C   PK             KP P  PK  P  PK
Sbjct: 1   MASNTKLIATILLLSILSYSTFTGANCPPSPKPTPPSPKPTPPSPKPTPPTPKPTPPSPK 60

Query: 61  IIRP-----PSASSPAVQSSYCPKDTVKFGVCTDILG-IGSVIGSPVSKKCCSLLSGLTN 120
              P     PS S      S CPKDT+K G C D+LG +  +IG+P S KCC+L+ GL +
Sbjct: 61  PTPPSPKPTPSPSPSPPSQSKCPKDTLKLGACADLLGLVNIIIGTPPSSKCCALIKGLAD 120

Query: 121 VEAAACLCTAIKANVLGIDLDIPVSLSLLISSCKKSVPTGYQC 145
           +EAA CLCTAIKANVLGI+L++PV+LSL++S+C+K+VPTG+QC
Sbjct: 121 LEAALCLCTAIKANVLGINLNVPVTLSLILSACQKTVPTGFQC 163

The following BLAST results are available for this feature:

BLAST of Lsi08G012150 vs. Swiss-Prot


Match Name	E-value	Identity	Description
14KD_DAUCA	7.5e-23	42.76	14 kDa proline-rich protein DC2.15 OS=Daucus carota PE=2 SV=1	[more]
ERLI1_ARATH	2.2e-22	40.83	Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana GN=EARLI1 PE=1 SV=1	[more]
ERLL1_ARATH	6.4e-22	40.85	pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana GN=AZI1 PE=1 SV=1	[more]
LBP65_ARATH	4.6e-20	53.13	Putative lipid-binding protein At4g00165 OS=Arabidopsis thaliana GN=At4g00165 PE...	[more]
ERLL3_ARATH	1.0e-19	35.63	pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana GN=At4g12500 PE=2 ...	[more]

BLAST of Lsi08G012150 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPN2_CUCSA	1.5e-57	83.56	Proline-rich protein DC2.15 OS=Cucumis sativus GN=Csa_2G381890 PE=4 SV=1	[more]
G7I725_MEDTR	4.1e-28	50.32	Protease inhibitor/seed storage/LTP family protein OS=Medicago truncatula GN=MTR...	[more]
A0A061GJ47_THECC	5.4e-28	53.42	Bimodular protein OS=Theobroma cacao GN=TCM_036975 PE=4 SV=1	[more]
A0A151TT59_CAJCA	7.1e-28	47.85	14 kDa proline-rich protein DC2.15 OS=Cajanus cajan GN=KK1_009402 PE=4 SV=1	[more]
G7I723_MEDTR	9.2e-28	49.68	Lipid transfer protein OS=Medicago truncatula GN=MTR_1g012690 PE=4 SV=1	[more]

BLAST of Lsi08G012150 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45180.1	1.0e-25	46.90	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi...	[more]
AT1G62510.1	6.5e-25	44.37	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi...	[more]
AT1G12090.1	2.5e-24	44.06	extensin-like protein	[more]
AT4G12480.1	1.2e-23	40.83	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi...	[more]
AT4G12470.1	3.6e-23	40.85	azelaic acid induced 1	[more]

BLAST of Lsi08G012150 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659088926\|ref\|XP_008445235.1\|	1.9e-58	84.93	PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis melo]	[more]
gi\|449441902\|ref\|XP_004138721.1\|	2.1e-57	83.56	PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis sativus]	[more]
gi\|357437019\|ref\|XP_003588785.1\|	6.0e-28	50.32	protease inhibitor/seed storage/LTP family protein [Medicago truncatula]	[more]
gi\|590572334\|ref\|XP_007011824.1\|	7.8e-28	53.42	Bimodular protein [Theobroma cacao]	[more]
gi\|1012359007\|gb\|KYP70191.1\|	1.0e-27	47.85	14 kDa proline-rich protein DC2.15 [Cajanus cajan]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR027923	Hydrophob_seed
IPR016140	Bifunc_inhib/LTP/seed_store

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G012150.1	Lsi08G012150.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 64..144 score: 1.
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	unknown	SSF47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 67..144 score: 2.76
IPR027923	Hydrophobic seed protein	PFAM	PF14547	Hydrophob_seed	coord: 64..144 score: 1.0
None	No IPR available	GENE3D	G3DSA:1.10.110.10		coord: 63..144 score: 3.9
None	No IPR available	PANTHER	PTHR31731	FAMILY NOT NAMED	coord: 5..144 score: 4.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi08G012150	Csa2G381890	Cucumber (Chinese Long) v2	culsiB140
Lsi08G012150	CSPI02G23640	Wild cucumber (PI 183967)	cpilsiB139
Lsi08G012150	Cucsa.162540	Cucumber (Gy14) v1	cgylsiB267
Lsi08G012150	CmaCh12G003450	Cucurbita maxima (Rimu)	cmalsiB184
Lsi08G012150	CmoCh12G002950	Cucurbita moschata (Rifu)	cmolsiB175
Lsi08G012150	Cla022335	Watermelon (97103) v1	lsiwmB465
Lsi08G012150	MELO3C011271	Melon (DHL92) v3.5.1	lsimeB474
Lsi08G012150	MELO3C011271.2	Melon (DHL92) v3.6.1	lsimedB493
Lsi08G012150	Cla97C08G156820	Watermelon (97103) v2	lsiwmbB444
Lsi08G012150	Bhi04G000007	Wax gourd	lsiwgoB592
Lsi08G012150	CsGy2G023420	Cucumber (Gy14) v2	cgyblsiB126
Lsi08G012150	CsaV3_2G032190	Cucumber (Chinese Long) v3	cuclsiB152

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi08G012150	Bottle gourd (USVL1VR-Ls)	lsilsiB151
Lsi08G012150	Cucumber (Gy14) v1	cgylsiB116
Lsi08G012150	Cucurbita maxima (Rimu)	cmalsiB039
Lsi08G012150	Cucurbita maxima (Rimu)	cmalsiB460
Lsi08G012150	Cucurbita maxima (Rimu)	cmalsiB739
Lsi08G012150	Cucurbita moschata (Rifu)	cmolsiB027
Lsi08G012150	Cucurbita moschata (Rifu)	cmolsiB070
Lsi08G012150	Cucurbita moschata (Rifu)	cmolsiB445
Lsi08G012150	Cucurbita moschata (Rifu)	cmolsiB731
Lsi08G012150	Cucurbita pepo (Zucchini)	cpelsiB100
Lsi08G012150	Cucurbita pepo (Zucchini)	cpelsiB651
Lsi08G012150	Cucurbita pepo (Zucchini)	cpelsiB685
Lsi08G012150	Wild cucumber (PI 183967)	cpilsiB140
Lsi08G012150	Wild cucumber (PI 183967)	cpilsiB317
Lsi08G012150	Watermelon (Charleston Gray)	lsiwcgB422
Lsi08G012150	Watermelon (Charleston Gray)	lsiwcgB434
Lsi08G012150	Watermelon (Charleston Gray)	lsiwcgB438
Lsi08G012150	Watermelon (97103) v1	lsiwmB479
Lsi08G012150	Watermelon (97103) v1	lsiwmB484
Lsi08G012150	Cucumber (Gy14) v2	cgyblsiB128
Lsi08G012150	Silver-seed gourd	carlsiB128
Lsi08G012150	Silver-seed gourd	carlsiB580
Lsi08G012150	Silver-seed gourd	carlsiB898
Lsi08G012150	Cucumber (Chinese Long) v3	cuclsiB154
Lsi08G012150	Cucumber (Chinese Long) v3	cuclsiB337
Lsi08G012150	Watermelon (97103) v2	lsiwmbB430