Lsi08G003900 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi08G003900
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptiontRNA dimethylallyltransferase 2
Locationchr08 : 10832506 .. 10833468 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACACTTTCCCTCCCCTTCTCCCACTCTTTTCCTCTTTCTCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGTTTCCTCCGATTCCCTCCTCCTCCTCCTTGGCGCTACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGGGGCCTCGACATCACCACTAACAAGCTCCCTCTCTCCCACCGCCGCGCCGTCCCCCATCATCTTCTTGGCGATTTCGACCCTCTCGCCGGTGAGGTTTCTCCTGCCGCCTTCCGTTCTCATGCCTCCGCCGTCATCTCCGGCGTCGTCTCCCGAGGCAACCTTCCTCTTGTCGTTGGCGGCTCCAACTCCTTCATTCACGCTCTCGTTGCCGCTGATTTCGATGCTACCCAGGACGTTTTCGGCGGGCCTCGGAATCGGGTGTCGGCGGAGTTTCGGTATCGGTGCTGCTTTTTGTGGGTCGACGTGTCGTTTCCGGTTCTGAAACGGTATTTGTCGAATCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGGGGAGTTGGCGGAGTTTTATGACCCGGAAATGGCTGATTCGGAGCCCCGAACCGGGCTGAGAAAAGCCATTGGAGTGCCCGAATTCGAGGAGTTTTTCCGGCGGCATCATCCGAAAGGGAGGGATTACAGAGAGGGGGATCCACTGCGGGCGAGCGCATACGAAGAAGCGGTAAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGTGGAAGATCGGACGGCTCAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTCCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGAGCTTCATTCAGGAGTAG

mRNA sequence

ATGACACTTTCCCTCCCCTTCTCCCACTCTTTTCCTCTTTCTCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGTTTCCTCCGATTCCCTCCTCCTCCTCCTTGGCGCTACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGGGGCCTCGACATCACCACTAACAAGCTCCCTCTCTCCCACCGCCGCGCCGTCCCCCATCATCTTCTTGGCGATTTCGACCCTCTCGCCGGTGAGGTTTCTCCTGCCGCCTTCCGTTCTCATGCCTCCGCCGTCATCTCCGGCGTCGTCTCCCGAGGCAACCTTCCTCTTGTCGTTGGCGGCTCCAACTCCTTCATTCACGCTCTCGTTGCCGCTGATTTCGATGCTACCCAGGACGTTTTCGGCGGGCCTCGGAATCGGGTGTCGGCGGAGTTTCGGTATCGGTGCTGCTTTTTGTGGGTCGACGTGTCGTTTCCGGTTCTGAAACGGTATTTGTCGAATCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGGGGAGTTGGCGGAGTTTTATGACCCGGAAATGGCTGATTCGGAGCCCCGAACCGGGCTGAGAAAAGCCATTGGAGTGCCCGAATTCGAGGAGTTTTTCCGGCGGCATCATCCGAAAGGGAGGGATTACAGAGAGGGGGATCCACTGCGGGCGAGCGCATACGAAGAAGCGGTAAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGTGGAAGATCGGACGGCTCAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTCCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGAGCTTCATTCAGGAGTAG

Coding sequence (CDS)

ATGACACTTTCCCTCCCCTTCTCCCACTCTTTTCCTCTTTCTCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGTTTCCTCCGATTCCCTCCTCCTCCTCCTTGGCGCTACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGGGGCCTCGACATCACCACTAACAAGCTCCCTCTCTCCCACCGCCGCGCCGTCCCCCATCATCTTCTTGGCGATTTCGACCCTCTCGCCGGTGAGGTTTCTCCTGCCGCCTTCCGTTCTCATGCCTCCGCCGTCATCTCCGGCGTCGTCTCCCGAGGCAACCTTCCTCTTGTCGTTGGCGGCTCCAACTCCTTCATTCACGCTCTCGTTGCCGCTGATTTCGATGCTACCCAGGACGTTTTCGGCGGGCCTCGGAATCGGGTGTCGGCGGAGTTTCGGTATCGGTGCTGCTTTTTGTGGGTCGACGTGTCGTTTCCGGTTCTGAAACGGTATTTGTCGAATCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGGGGAGTTGGCGGAGTTTTATGACCCGGAAATGGCTGATTCGGAGCCCCGAACCGGGCTGAGAAAAGCCATTGGAGTGCCCGAATTCGAGGAGTTTTTCCGGCGGCATCATCCGAAAGGGAGGGATTACAGAGAGGGGGATCCACTGCGGGCGAGCGCATACGAAGAAGCGGTAAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGTGGAAGATCGGACGGCTCAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTCCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGAGCTTCATTCAGGAGTAG

Protein sequence

MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQE
Homology
[Close]
BLAST of Lsi08G003900 vs. Swiss-Prot
Match: IPT_HUMLU (Adenylate isopentenyltransferase OS=Humulus lupulus PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 8.6e-88
Identity = 177/301 (58.80%), Postives = 216/301 (71.76%), Query Frame = 1 

Query: 29  DSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHL 88
           + LL+L+GATG+GKS LSIDLAA FP E+INSDKMQ+Y+GLDITTNK+ +  R  VPHHL
Sbjct: 30  EKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 89

Query: 89  LGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDAT-QDVF 148
           LG+ DP  GE++PA FRS A   +S +  R  LP++VGGSNSFIHAL+   FD++   VF
Sbjct: 90  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVF 149

Query: 149 -GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPE----M 208
             G  + VS+E RY CCFLWVDVS  VL  YL+ RVDDMLE GM  ELAEFY PE     
Sbjct: 150 EEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHD 209

Query: 209 ADSEPRTGLRKAIGVPEFEEFFRRHHP---KGRDYREGDPLRASAYEEAVRKIKENTWQL 268
            DS  RTGLRKAIGVPEF+ +F +  P   +G D    D +R  A+EEAVR IKENT  L
Sbjct: 210 EDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGR-DRVRRGAFEEAVRAIKENTCHL 269

Query: 269 TKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQ 321
            KRQ+ KI RL+ AGWDL+R+DAT +  A + S++ EK +EIWE QV+EPSVKIV  F+ 
Sbjct: 270 AKRQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFLD 329

[Close]
BLAST of Lsi08G003900 vs. Swiss-Prot
Match: IPT1_ARATH (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana GN=IPT1 PE=1 SV=2)

HSP 1 Score: 306.6 bits (784), Expect = 3.2e-82
Identity = 162/315 (51.43%), Postives = 216/315 (68.57%), Query Frame = 1 

Query: 10  SFPLSRRPRCPLILS--------SVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+ S            D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNR 189
           P++ GGSNSF+HAL+A  FD   D F      +S++ RY CCF+WVDVS  VL  YL  R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLR 249
           VD+M+++GM  EL+ FYDP  +  E R G+RKAIGVPEF+ +F+ + P+ +  +  D LR
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPEKKMIK-WDALR 277

Query: 250 ASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKR-SE 309
            +AY++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+   ++EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

[Close]
BLAST of Lsi08G003900 vs. Swiss-Prot
Match: IPT6_ARATH (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana GN=IPT6 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-75
Identity = 155/299 (51.84%), Postives = 205/299 (68.56%), Query Frame = 1 

Query: 29  DSLLLLLGATGSGKSSLSIDLAARF-PSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 88
           D ++L+ G TG+GKS LS+DLA RF P+EIINSDKMQ+Y+G +I TN +PL  +  VPHH
Sbjct: 45  DKVVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHH 104

Query: 89  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 148
           LLG F P  GE++PA FRS A+  IS ++S   LP+VVGGSNSF HAL+A  FD   D F
Sbjct: 105 LLGQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPF 164

Query: 149 --GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADS 208
             G   + + ++ RY+CC LWVDV  PVL ++L NRVD M+E+G+V +LAE YDP + DS
Sbjct: 165 SPGSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDS 224

Query: 209 EPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLW 268
             R G+RK IGV EF+ +F R +PK  D    D  R +AYEE V+ +KE T +L K+Q  
Sbjct: 225 GRRLGVRKTIGVEEFDRYF-RVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKE 284

Query: 269 KIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRS----EIWETQVVEPSVKIVKSFIQE 321
           KI +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 285 KIMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]
BLAST of Lsi08G003900 vs. Swiss-Prot
Match: IPT4_ARATH (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana GN=IPT4 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 149/298 (50.00%), Postives = 204/298 (68.46%), Query Frame = 1 

Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS+IHAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEP 207
              +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  EP
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKI 267
             G+ KAIGV EF+++ +  +    D  + DP+R  AYE+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-AAEKRSEIWETQVVEPSVKIVKSFIQE 321
            +LR AGWD+K+VDATA    A+ A    E  AE + +IW  +V+EP VKIV+S + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]
BLAST of Lsi08G003900 vs. Swiss-Prot
Match: IPT8_ARATH (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana GN=IPT8 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.7e-72
Identity = 144/309 (46.60%), Postives = 198/309 (64.08%), Query Frame = 1 

Query: 12  PLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDI 71
           P S  P   + +       +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +
Sbjct: 26  PRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKV 85

Query: 72  TTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSF 131
           TTN++ +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF
Sbjct: 86  TTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSF 145

Query: 132 IHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMV 191
           IHAL+   FD     F      +S+  RY CCFLWVDVS  VL  YLS RVD M+E+GM 
Sbjct: 146 IHALLVDRFDPKTYPFSS-ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMF 205

Query: 192 GELAEFYDPEMADSEPRT-GLRKAIGVPEFEEFFRRHHPKGRD-YREGDPLRASAYEEAV 251
            ELA FYDP  + S  R  G+ K IG+PEF+ +F  + P+ +    E D  R  AY+EAV
Sbjct: 206 EELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAV 265

Query: 252 RKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEP 311
           ++IKENTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ 
Sbjct: 266 QEIKENTWRLAKKQIERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDE 325

Query: 312 SVKIVKSFI 319
           S+K+VK F+
Sbjct: 326 SIKVVKRFL 325

[Close]
BLAST of Lsi08G003900 vs. TrEMBL
Match: A0A0A0K943_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 6.1e-165
Identity = 297/320 (92.81%), Postives = 305/320 (95.31%), Query Frame = 1 

Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPLILSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSF+HALVAADFDATQDVF G RNRVS EF YRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GMV ELA+FYDPEMADSE R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +A A+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]
BLAST of Lsi08G003900 vs. TrEMBL
Match: V7BDD3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G028100g PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-91
Identity = 183/312 (58.65%), Postives = 226/312 (72.44%), Query Frame = 1 

Query: 11  FPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFP-SEIINSDKMQLYRGL 70
           +P+ R PR          D +++++GATGSGKS LSIDLA  FP SEIINSDKMQ+YRGL
Sbjct: 15  YPIRRWPRMDSSFHR-RKDKVVVIMGATGSGKSRLSIDLATLFPVSEIINSDKMQVYRGL 74

Query: 71  DITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSN 130
           DITTNK+P + RRAVPHHLLGD DP   E SPA FR  A  +I  +  R +LP+VVGGSN
Sbjct: 75  DITTNKIPHAQRRAVPHHLLGDVDPSLPEFSPADFRRRAGHLIEDINRRDSLPIVVGGSN 134

Query: 131 SFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAG 190
           SF+HAL+  DF    +VF      +S+E RYRCCFLWVD++FPVL +YL +RVDDMLE+G
Sbjct: 135 SFVHALLVQDFQPDSNVFRQGEELISSELRYRCCFLWVDIAFPVLSQYLCDRVDDMLESG 194

Query: 191 MVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREG-DPLRASAYEEA 250
           MV ELA+F+DPE A  E  +G+RKAIGVPEF+ +F ++ P       G DPLR  AYEEA
Sbjct: 195 MVDELAQFFDPE-ASRESESGIRKAIGVPEFDRYFNKYPPWAGPAGPGQDPLRERAYEEA 254

Query: 251 VRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVE 310
           VR IKENT +L +RQ+ KI RL+RAGWDL+R+DAT A   VL S  +   S++WE QV+E
Sbjct: 255 VRAIKENTCELAERQMEKIERLKRAGWDLRRIDATEAFRMVLTS-GSNHGSDVWERQVLE 314

Query: 311 PSVKIVKSFIQE 321
           PSVKIVK F+ E
Sbjct: 315 PSVKIVKRFLME 323

[Close]
BLAST of Lsi08G003900 vs. TrEMBL
Match: A0A059BGB7_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_G02313 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 5.8e-91
Identity = 179/307 (58.31%), Postives = 219/307 (71.34%), Query Frame = 1 

Query: 31  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLG 90
           +++++GATG GKS LSIDLA+ FPSE+INSDKMQ+YRGLDITTNK+  + RR VPHHLLG
Sbjct: 1   VVVIMGATGCGKSGLSIDLASVFPSEVINSDKMQVYRGLDITTNKITPAERRGVPHHLLG 60

Query: 91  DFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGP 150
            FDP  GE+SP+ FRS   + ISG+ SRGNLPL+ GGSNS IHAL+   FD   DVFGG 
Sbjct: 61  SFDPDDGEMSPSMFRSLGQSAISGIASRGNLPLLAGGSNSLIHALLVERFDPEVDVFGGL 120

Query: 151 RNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTG 210
            +  S E RY CCFLWVDVS PVL+ YLS RVDDML+ GM  ELA +YDP   D   RTG
Sbjct: 121 TSPASLELRYNCCFLWVDVSPPVLEEYLSKRVDDMLDMGMFEELARYYDPRRTDPGGRTG 180

Query: 211 LRKAIGVPEFEEFFRRHHP------------KGRDYREGDPLRA--SAYEEAVRKIKENT 270
           LRKAIGVPEF+ +F++H P            +  + REGD  RA   AYEEAV +IKENT
Sbjct: 181 LRKAIGVPEFDRYFKKHPPQRWAGQGQGDARRPEEGREGDDDRAREGAYEEAVWEIKENT 240

Query: 271 WQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE---AAEKRSEIWETQVVEPSVKI 321
            QL KRQ+ KI RLR  GW+L+++DAT   +  +A++      +R ++WE QVV PSVKI
Sbjct: 241 RQLAKRQIEKIERLRECGWELRKLDATETFLLAMATDEGGGGGRRRDVWERQVVGPSVKI 300

[Close]
BLAST of Lsi08G003900 vs. TrEMBL
Match: G7ID98_MEDTR (Adenylate isopentenyltransferase OS=Medicago truncatula GN=MTR_1g110590 PE=4 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 7.6e-91
Identity = 188/333 (56.46%), Postives = 238/333 (71.47%), Query Frame = 1 

Query: 2   TLSLPFSHSFPLSRR-PRCPLILSSVS----SDSLLLLLGATGSGKSSLSIDLAARFP-S 61
           TL  P S S   +RR P CP +  S S     D +L+++GATGSGKS LS++LA  FP S
Sbjct: 32  TLFTPSSSSATHTRRRPHCPRMEISPSRHRRKDKVLIIVGATGSGKSRLSVELATLFPYS 91

Query: 62  EIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV 121
           EIINSDK+Q+YRGLDITTNK+P   R  VPHHLLGD DP  GE SP+ FR HA  +IS +
Sbjct: 92  EIINSDKIQVYRGLDITTNKIPFHQRNNVPHHLLGDIDPSHGEFSPSDFRRHAGDIISDI 151

Query: 122 VSRGNLPLVVGGSNSFIHALVAADFDATQDVF---GGPRNRVSAEFRYRCCFLWVDVSFP 181
            SR  LP++VGGSNSFIHAL+   FD   +VF         +S++ RY+CCFLW+D+SFP
Sbjct: 152 TSRRKLPIIVGGSNSFIHALLVERFDPESNVFDESSSLSTSISSDLRYKCCFLWMDISFP 211

Query: 182 VLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPK-G 241
           VL  YL  RVDDM ++GMV ELAEFY+P   D++ +TGLRKAIGVPEF+ FF+++ P+ G
Sbjct: 212 VLSEYLLKRVDDMFDSGMVNELAEFYEP---DADNQTGLRKAIGVPEFDRFFKQYPPQVG 271

Query: 242 RDYRE-GDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVL 301
            D  E  +P+R  AY EAV+ IK+NT QL KRQ+ KI RL+RAGWDL+R+DAT A  AVL
Sbjct: 272 PDESERHNPMREGAYIEAVKAIKDNTCQLAKRQIGKILRLKRAGWDLQRIDATEAFRAVL 331

Query: 302 ASEA---AEKRSEIWETQVVEPSVKIVKSFIQE 321
            SE+    E+ + +W+ QV+EPSVKIVK F+ E
Sbjct: 332 TSESNGGGEEFTGVWKKQVLEPSVKIVKRFLME 361

[Close]
BLAST of Lsi08G003900 vs. TrEMBL
Match: A0A0S3SLQ5_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.08G030100 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.4e-90
Identity = 180/311 (57.88%), Postives = 222/311 (71.38%), Query Frame = 1 

Query: 12  PLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFP-SEIINSDKMQLYRGLD 71
           P+ R PR          D +++++GATGSGKS LSIDLA  FP SEIINSDKMQ+YRGLD
Sbjct: 16  PIKRWPRMDASFHR-RKDKVVVIMGATGSGKSRLSIDLATLFPVSEIINSDKMQVYRGLD 75

Query: 72  ITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNS 131
           ITTNK+PL+ RR VPHHLLGD DP   E SPA FR  A  +I  + SR  LP+VVGGSNS
Sbjct: 76  ITTNKIPLAQRRGVPHHLLGDVDPSLPEFSPADFRRRAGHIIDDISSRDCLPIVVGGSNS 135

Query: 132 FIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGM 191
           F+HAL+  DF    +VF      +S+E RY+CCFLWVD++FPVL +YL +RVDDML++GM
Sbjct: 136 FVHALLVEDFQPDSNVFQHAERLISSELRYKCCFLWVDIAFPVLSQYLCHRVDDMLQSGM 195

Query: 192 VGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREG-DPLRASAYEEAV 251
           V ELA+F+DPE A     +GLRKAIGVPEF+ +F  + P       G DPLR  AYEEAV
Sbjct: 196 VQELAQFFDPE-ASRRTGSGLRKAIGVPEFDRYFNMYPPWAGPAGPGEDPLRKRAYEEAV 255

Query: 252 RKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEP 311
           R IK+NT +L +RQ+ KI RL RAGWDL+R+DAT A   VL S  +   S++WE QV+EP
Sbjct: 256 RAIKDNTCELAERQMEKIERLMRAGWDLRRIDATEAFGVVLTS-GSNNGSDVWERQVLEP 315

Query: 312 SVKIVKSFIQE 321
           SVKIV+ F+ E
Sbjct: 316 SVKIVRRFLME 323

[Close]
BLAST of Lsi08G003900 vs. TAIR10
Match: AT1G68460.1 (AT1G68460.1 isopentenyltransferase 1)

HSP 1 Score: 306.6 bits (784), Expect = 1.8e-83
Identity = 162/315 (51.43%), Postives = 216/315 (68.57%), Query Frame = 1 

Query: 10  SFPLSRRPRCPLILS--------SVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+ S            D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNR 189
           P++ GGSNSF+HAL+A  FD   D F      +S++ RY CCF+WVDVS  VL  YL  R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLR 249
           VD+M+++GM  EL+ FYDP  +  E R G+RKAIGVPEF+ +F+ + P+ +  +  D LR
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPEKKMIK-WDALR 277

Query: 250 ASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKR-SE 309
            +AY++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+   ++EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

[Close]
BLAST of Lsi08G003900 vs. TAIR10
Match: AT1G25410.1 (AT1G25410.1 isopentenyltransferase 6)

HSP 1 Score: 284.3 bits (726), Expect = 9.5e-77
Identity = 155/299 (51.84%), Postives = 205/299 (68.56%), Query Frame = 1 

Query: 29  DSLLLLLGATGSGKSSLSIDLAARF-PSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 88
           D ++L+ G TG+GKS LS+DLA RF P+EIINSDKMQ+Y+G +I TN +PL  +  VPHH
Sbjct: 45  DKVVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHH 104

Query: 89  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 148
           LLG F P  GE++PA FRS A+  IS ++S   LP+VVGGSNSF HAL+A  FD   D F
Sbjct: 105 LLGQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPF 164

Query: 149 --GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADS 208
             G   + + ++ RY+CC LWVDV  PVL ++L NRVD M+E+G+V +LAE YDP + DS
Sbjct: 165 SPGSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDS 224

Query: 209 EPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLW 268
             R G+RK IGV EF+ +F R +PK  D    D  R +AYEE V+ +KE T +L K+Q  
Sbjct: 225 GRRLGVRKTIGVEEFDRYF-RVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKE 284

Query: 269 KIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRS----EIWETQVVEPSVKIVKSFIQE 321
           KI +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 285 KIMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]
BLAST of Lsi08G003900 vs. TAIR10
Match: AT4G24650.1 (AT4G24650.1 isopentenyltransferase 4)

HSP 1 Score: 281.6 bits (719), Expect = 6.2e-76
Identity = 149/298 (50.00%), Postives = 204/298 (68.46%), Query Frame = 1 

Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS+IHAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEP 207
              +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  EP
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKI 267
             G+ KAIGV EF+++ +  +    D  + DP+R  AYE+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-AAEKRSEIWETQVVEPSVKIVKSFIQE 321
            +LR AGWD+K+VDATA    A+ A    E  AE + +IW  +V+EP VKIV+S + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]
BLAST of Lsi08G003900 vs. TAIR10
Match: AT3G19160.1 (AT3G19160.1 ATP/ADP isopentenyltransferases)

HSP 1 Score: 274.2 bits (700), Expect = 9.9e-74
Identity = 144/309 (46.60%), Postives = 198/309 (64.08%), Query Frame = 1 

Query: 12  PLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDI 71
           P S  P   + +       +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +
Sbjct: 26  PRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKV 85

Query: 72  TTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSF 131
           TTN++ +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF
Sbjct: 86  TTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSF 145

Query: 132 IHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMV 191
           IHAL+   FD     F      +S+  RY CCFLWVDVS  VL  YLS RVD M+E+GM 
Sbjct: 146 IHALLVDRFDPKTYPFSS-ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMF 205

Query: 192 GELAEFYDPEMADSEPRT-GLRKAIGVPEFEEFFRRHHPKGRD-YREGDPLRASAYEEAV 251
            ELA FYDP  + S  R  G+ K IG+PEF+ +F  + P+ +    E D  R  AY+EAV
Sbjct: 206 EELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAV 265

Query: 252 RKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEP 311
           ++IKENTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ 
Sbjct: 266 QEIKENTWRLAKKQIERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDE 325

Query: 312 SVKIVKSFI 319
           S+K+VK F+
Sbjct: 326 SIKVVKRFL 325

[Close]
BLAST of Lsi08G003900 vs. TAIR10
Match: AT5G19040.1 (AT5G19040.1 isopentenyltransferase 5)

HSP 1 Score: 210.7 bits (535), Expect = 1.3e-54
Identity = 122/294 (41.50%), Postives = 167/294 (56.80%), Query Frame = 1 

Query: 29  DSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHL 88
           D ++ ++GATG+GKS L+IDLA RFP+EI+NSDK+Q+Y+GLDI TNK+       VPHHL
Sbjct: 33  DKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPHHL 92

Query: 89  LGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFG 148
           LG       + +   F+  A   +  +V R  +P++ GGSNS+I ALV            
Sbjct: 93  LGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV------------ 152

Query: 149 GPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPR 208
              + V    RY CCFLWVDVS PVL  ++S RVD M++ G+V E+   +DP  +D    
Sbjct: 153 --NDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYS-- 212

Query: 209 TGLRKAIGVPEFEEFFR---RHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLW 268
            G+R+AIGVPE +EF R   R++P     R          E A+ KIKENT  L  RQL 
Sbjct: 213 AGIRRAIGVPELDEFLRSEMRNYPAETTER--------LLETAIEKIKENTCLLACRQLQ 272

Query: 269 KIGRL-RRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEPSVKIVKSFI 319
           KI RL ++  W++ RVDAT   +        E+  E W+  V  PS   V+ F+
Sbjct: 273 KIQRLYKQWKWNMHRVDATEVFL-----RRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]
BLAST of Lsi08G003900 vs. NCBI nr
Match: gi|659089679|ref|XP_008445640.1| (PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo])

HSP 1 Score: 593.2 bits (1528), Expect = 2.7e-166
Identity = 298/320 (93.12%), Postives = 308/320 (96.25%), Query Frame = 1 

Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFPLSRRPRCPL+LSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSFIHALVAADFD TQDVF G RNRVSAEFRYRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GM+ ELA+FYDPEMAD E R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +ASA+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]
BLAST of Lsi08G003900 vs. NCBI nr
Match: gi|449466400|ref|XP_004150914.1| (PREDICTED: adenylate isopentenyltransferase-like [Cucumis sativus])

HSP 1 Score: 588.2 bits (1515), Expect = 8.7e-165
Identity = 297/320 (92.81%), Postives = 305/320 (95.31%), Query Frame = 1 

Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPLILSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSF+HALVAADFDATQDVF G RNRVS EF YRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GMV ELA+FYDPEMADSE R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +A A+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]
BLAST of Lsi08G003900 vs. NCBI nr
Match: gi|702420829|ref|XP_010066714.1| (PREDICTED: adenylate isopentenyltransferase [Eucalyptus grandis])

HSP 1 Score: 345.5 bits (885), Expect = 9.9e-92
Identity = 192/347 (55.33%), Postives = 232/347 (66.86%), Query Frame = 1 

Query: 3   LSLPFSHSFP--LSRRPRCPLILSSVSSDSLL----------LLLGATGSGKSSLSIDLA 62
           L  P +H  P   S  PR P   S V+S S L          +++GATG GKS LSIDLA
Sbjct: 25  LFTPNNHHHPNGSSAHPRPPARWSPVASSSSLAAARRRAKVVVIMGATGCGKSGLSIDLA 84

Query: 63  ARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASA 122
           + FPSE+INSDKMQ+YRGLDITTNK+  + RR VPHHLLG FDP  GE+SP+ FRS   +
Sbjct: 85  SVFPSEVINSDKMQVYRGLDITTNKITPAERRGVPHHLLGSFDPDDGEMSPSMFRSLGQS 144

Query: 123 VISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVS 182
            ISG+ SRGNLPL+ GGSNS IHAL+   FD   DVFGG  +  S E RY CCFLWVDVS
Sbjct: 145 AISGIASRGNLPLLAGGSNSLIHALLVERFDPEVDVFGGLTSPASLELRYNCCFLWVDVS 204

Query: 183 FPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHP- 242
            PVL+ YLS RVDDML+ GM  ELA +YDP   D   RTGLRKAIGVPEF+ +F++H P 
Sbjct: 205 PPVLEEYLSKRVDDMLDMGMFEELARYYDPRRTDPGGRTGLRKAIGVPEFDRYFKKHPPQ 264

Query: 243 -----------KGRDYREGDPLRA--SAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWD 302
                      +  + REGD  RA   AYEEAV +IKENT QL KRQ+ KI RLR  GW+
Sbjct: 265 RWAGQGQGDARRPEEGREGDDDRAREGAYEEAVWEIKENTRQLAKRQIEKIERLRECGWE 324

Query: 303 LKRVDATAAVVAVLASE---AAEKRSEIWETQVVEPSVKIVKSFIQE 321
           L+++DAT   +  +A++      +R ++WE QVV PSVKIVK F+QE
Sbjct: 325 LRKLDATETFLLAMATDEGGGGGRRRDVWERQVVGPSVKIVKRFLQE 371

[Close]
BLAST of Lsi08G003900 vs. NCBI nr
Match: gi|828305886|ref|XP_012570225.1| (PREDICTED: adenylate isopentenyltransferase [Cicer arietinum])

HSP 1 Score: 344.7 bits (883), Expect = 1.7e-91
Identity = 187/336 (55.65%), Postives = 238/336 (70.83%), Query Frame = 1 

Query: 1   MTLSLPFSHSFPLSR-RPRCPLILSSVS----SDSLLLLLGATGSGKSSLSIDLAARFP- 60
           +TL +P S S   SR +PR P + S+ S     D +++++GATGSGKS LS++LA  FP 
Sbjct: 27  LTLFIPSSSSSSSSRTKPRWPRMDSAASRHRRKDKVVVIMGATGSGKSRLSVELATLFPF 86

Query: 61  SEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISG 120
           SEIINSDKMQ+YRGLDITTNK+P   R  VPHHLLGD D   GE SP+ FR HA  +IS 
Sbjct: 87  SEIINSDKMQVYRGLDITTNKIPFHQRNGVPHHLLGDVDHSHGEFSPSDFRRHAGEIISD 146

Query: 121 VVSRGNLPLVVGGSNSFIHALVAADFDATQDVF-------GGPRNRVSAEFRYRCCFLWV 180
           + SR  LP++VGGSNSFIHAL+   FD   +VF           + +S++ RY+CCFLW+
Sbjct: 147 ITSRRKLPIIVGGSNSFIHALLVERFDPELNVFDKSSSSSSSSLSSISSDLRYKCCFLWM 206

Query: 181 DVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRH 240
           D+SFPVL  YL  RVD+M ++GMV ELA+F++P   DSE RTGLRKAIGVPEF+ FF ++
Sbjct: 207 DISFPVLSEYLLKRVDEMFDSGMVDELAQFFEP---DSENRTGLRKAIGVPEFDRFFNKY 266

Query: 241 HPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVV 300
            P  +     +P+R  AYEEAVR IK+NT QL KRQ+ KI RL+RAGWDL+R+DAT A  
Sbjct: 267 PPADKLEEGENPMREGAYEEAVRAIKDNTCQLAKRQIGKILRLKRAGWDLRRIDATEAFR 326

Query: 301 AVLASEA---AEKRSEIWETQVVEPSVKIVKSFIQE 321
           AVL SE+   A   S++W+ QV+EPSVKIVK F+ E
Sbjct: 327 AVLTSESRGGAGVFSDLWKNQVLEPSVKIVKRFLME 359

[Close]
BLAST of Lsi08G003900 vs. NCBI nr
Match: gi|593619595|ref|XP_007142919.1| (hypothetical protein PHAVU_007G028100g [Phaseolus vulgaris])

HSP 1 Score: 344.4 bits (882), Expect = 2.2e-91
Identity = 183/312 (58.65%), Postives = 226/312 (72.44%), Query Frame = 1 

Query: 11  FPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFP-SEIINSDKMQLYRGL 70
           +P+ R PR          D +++++GATGSGKS LSIDLA  FP SEIINSDKMQ+YRGL
Sbjct: 15  YPIRRWPRMDSSFHR-RKDKVVVIMGATGSGKSRLSIDLATLFPVSEIINSDKMQVYRGL 74

Query: 71  DITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSN 130
           DITTNK+P + RRAVPHHLLGD DP   E SPA FR  A  +I  +  R +LP+VVGGSN
Sbjct: 75  DITTNKIPHAQRRAVPHHLLGDVDPSLPEFSPADFRRRAGHLIEDINRRDSLPIVVGGSN 134

Query: 131 SFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAG 190
           SF+HAL+  DF    +VF      +S+E RYRCCFLWVD++FPVL +YL +RVDDMLE+G
Sbjct: 135 SFVHALLVQDFQPDSNVFRQGEELISSELRYRCCFLWVDIAFPVLSQYLCDRVDDMLESG 194

Query: 191 MVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREG-DPLRASAYEEA 250
           MV ELA+F+DPE A  E  +G+RKAIGVPEF+ +F ++ P       G DPLR  AYEEA
Sbjct: 195 MVDELAQFFDPE-ASRESESGIRKAIGVPEFDRYFNKYPPWAGPAGPGQDPLRERAYEEA 254

Query: 251 VRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVE 310
           VR IKENT +L +RQ+ KI RL+RAGWDL+R+DAT A   VL S  +   S++WE QV+E
Sbjct: 255 VRAIKENTCELAERQMEKIERLKRAGWDLRRIDATEAFRMVLTS-GSNHGSDVWERQVLE 314

Query: 311 PSVKIVKSFIQE 321
           PSVKIVK F+ E
Sbjct: 315 PSVKIVKRFLME 323

The following BLAST results are available for this feature:
BLAST of Lsi08G003900 vs. Swiss-Prot
Match NameE-valueIdentityDescription
IPT_HUMLU8.6e-8858.80Adenylate isopentenyltransferase OS=Humulus lupulus PE=1 SV=1[more]
IPT1_ARATH3.2e-8251.43Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana GN=IPT... [more]
IPT6_ARATH1.7e-7551.84Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana GN=IPT... [more]
IPT4_ARATH1.1e-7450.00Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana GN=IPT4 PE=1 SV=1[more]
IPT8_ARATH1.7e-7246.60Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana GN=IPT... [more]
BLAST of Lsi08G003900 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K943_CUCSA6.1e-16592.81Uncharacterized protein OS=Cucumis sativus GN=Csa_6G030440 PE=4 SV=1[more]
V7BDD3_PHAVU1.5e-9158.65Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G028100g PE=4 SV=1[more]
A0A059BGB7_EUCGR5.8e-9158.31Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_G02313 PE=4 ... [more]
G7ID98_MEDTR7.6e-9156.46Adenylate isopentenyltransferase OS=Medicago truncatula GN=MTR_1g110590 PE=4 SV=... [more]
A0A0S3SLQ5_PHAAN8.4e-9057.88Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.08G030100 PE=... [more]
BLAST of Lsi08G003900 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G68460.11.8e-8351.43 isopentenyltransferase 1[more]
AT1G25410.19.5e-7751.84 isopentenyltransferase 6[more]
AT4G24650.16.2e-7650.00 isopentenyltransferase 4[more]
AT3G19160.19.9e-7446.60 ATP/ADP isopentenyltransferases[more]
AT5G19040.11.3e-5441.50 isopentenyltransferase 5[more]
BLAST of Lsi08G003900 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659089679|ref|XP_008445640.1|2.7e-16693.13PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo][more]
gi|449466400|ref|XP_004150914.1|8.7e-16592.81PREDICTED: adenylate isopentenyltransferase-like [Cucumis sativus][more]
gi|702420829|ref|XP_010066714.1|9.9e-9255.33PREDICTED: adenylate isopentenyltransferase [Eucalyptus grandis][more]
gi|828305886|ref|XP_012570225.1|1.7e-9155.65PREDICTED: adenylate isopentenyltransferase [Cicer arietinum][more]
gi|593619595|ref|XP_007142919.1|2.2e-9158.65hypothetical protein PHAVU_007G028100g [Phaseolus vulgaris][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0008033tRNA processing
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR018022IPT
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034265 isopentenyl adenine biosynthetic process
biological_process GO:0006400 tRNA modification
biological_process GO:0008033 tRNA processing
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
molecular_function GO:0052623 ADP dimethylallyltransferase activity
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0052622 ATP dimethylallyltransferase activity
molecular_function GO:0052381 tRNA dimethylallyltransferase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G003900.1Lsi08G003900.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018022tRNA dimethylallyltransferasePFAMPF01715IPPTcoord: 64..138
score: 6.1E-16coord: 162..264
score: 8.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 29..144
score: 3.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 30..201
score: 7.78
NoneNo IPR availableGENE3DG3DSA:1.10.287.890coord: 173..264
score: 4.9
NoneNo IPR availablePANTHERPTHR11088TRNA DELTA 2 -ISOPENTENYLPYROPHOSPHATE TRANSFERASE-RELATEDcoord: 17..320
score: 9.0E
NoneNo IPR availablePANTHERPTHR11088:SF27ADENYLATE ISOPENTENYLTRANSFERASE 1, CHLOROPLASTIC-RELATEDcoord: 17..320
score: 9.0E