CSPI06G03050 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI06G03050
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptiontRNA isopentenyltransferase
LocationChr6 : 2519180 .. 2520269 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGGTAACCGTCGTCGTACGTAAAGAAAAAGGACTGATATTCCTCCGTTCCGTATATGTCTCTTCACATCAATAAACATTACCTCATCATCATCATCATCATCATCATCATTCACATTCTATGACACTTTCCCTCCCTTTCTCCCACTCTTTTCTTCTTTCCCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGCACCCTCCGATTCCCTCCTCCTACTTCTTGGCGCCACCGGCTCCGGCAAGTCTTCCCTCTCCATCGACCTCGCCGCTCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGAGGCCTCGATATCACCACTAACAAGCTTCCCCTCTCCCACCGCCGCGCGGTTCCTCATCATCTACTTGGCGATTTCGACCCTCTAGCTGGCGAGGTTTCTCCTGCTGCTTTCCGCTCCCATGCCTCCGCCGTCATCTCCGGCGTTATCTCCCGAGGTAACCTTCCACTAGTCGTTGGCGGCTCCAACTCCTTCGTTCACGCTCTTGTCGCCGCTGATTTCGATGCTACCCAAGACGTTTTCTGTGGGTCTAGGAATCGGGTGTCGACGGAGTTTCTGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCTGTTTTGAAGCGATATTTGTCGAAGCGGGTGGATGAGATGTTGGATGGTGGGATGGTGGAGGAGTTGGCGAAGTTTTATGACCCGGAAATGGCCGATTCGGAGACACGAGTGGGGCTGAGGAAAGCCATTGGAGTGCCAGAATTCGAGGAGTATTTCCGGCGGCATCACCCGAAAGGGAGGGATTACAGAGAGGGGGATCCGCTGCAGGCAAGTGCATTTGAAGAGGCGGTAAGGAAAATAAAAGAGAACACGTGGCAACTGACGAAAAGGCAGTTGTGGAAGATCGGACGGCTTAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTAGCGTCGGAAGTGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGGGCTTCATTCAGGAGTAGGTTTTCC

mRNA sequence

ATGACACTTTCCCTCCCTTTCTCCCACTCTTTTCTTCTTTCCCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGCACCCTCCGATTCCCTCCTCCTACTTCTTGGCGCCACCGGCTCCGGCAAGTCTTCCCTCTCCATCGACCTCGCCGCTCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGAGGCCTCGATATCACCACTAACAAGCTTCCCCTCTCCCACCGCCGCGCGGTTCCTCATCATCTACTTGGCGATTTCGACCCTCTAGCTGGCGAGGTTTCTCCTGCTGCTTTCCGCTCCCATGCCTCCGCCGTCATCTCCGGCGTTATCTCCCGAGGTAACCTTCCACTAGTCGTTGGCGGCTCCAACTCCTTCGTTCACGCTCTTGTCGCCGCTGATTTCGATGCTACCCAAGACGTTTTCTGTGGGTCTAGGAATCGGGTGTCGACGGAGTTTCTGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCTGTTTTGAAGCGATATTTGTCGAAGCGGGTGGATGAGATGTTGGATGGTGGGATGGTGGAGGAGTTGGCGAAGTTTTATGACCCGGAAATGGCCGATTCGGAGACACGAGTGGGGCTGAGGAAAGCCATTGGAGTGCCAGAATTCGAGGAGTATTTCCGGCGGCATCACCCGAAAGGGAGGGATTACAGAGAGGGGGATCCGCTGCAGGCAAGTGCATTTGAAGAGGCGGTAAGGAAAATAAAAGAGAACACGTGGCAACTGACGAAAAGGCAGTTGTGGAAGATCGGACGGCTTAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTAGCGTCGGAAGTGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGGGCTTCATTCAGGAGTAG

Coding sequence (CDS)

ATGACACTTTCCCTCCCTTTCTCCCACTCTTTTCTTCTTTCCCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGCACCCTCCGATTCCCTCCTCCTACTTCTTGGCGCCACCGGCTCCGGCAAGTCTTCCCTCTCCATCGACCTCGCCGCTCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGAGGCCTCGATATCACCACTAACAAGCTTCCCCTCTCCCACCGCCGCGCGGTTCCTCATCATCTACTTGGCGATTTCGACCCTCTAGCTGGCGAGGTTTCTCCTGCTGCTTTCCGCTCCCATGCCTCCGCCGTCATCTCCGGCGTTATCTCCCGAGGTAACCTTCCACTAGTCGTTGGCGGCTCCAACTCCTTCGTTCACGCTCTTGTCGCCGCTGATTTCGATGCTACCCAAGACGTTTTCTGTGGGTCTAGGAATCGGGTGTCGACGGAGTTTCTGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCTGTTTTGAAGCGATATTTGTCGAAGCGGGTGGATGAGATGTTGGATGGTGGGATGGTGGAGGAGTTGGCGAAGTTTTATGACCCGGAAATGGCCGATTCGGAGACACGAGTGGGGCTGAGGAAAGCCATTGGAGTGCCAGAATTCGAGGAGTATTTCCGGCGGCATCACCCGAAAGGGAGGGATTACAGAGAGGGGGATCCGCTGCAGGCAAGTGCATTTGAAGAGGCGGTAAGGAAAATAAAAGAGAACACGTGGCAACTGACGAAAAGGCAGTTGTGGAAGATCGGACGGCTTAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTAGCGTCGGAAGTGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGGGCTTCATTCAGGAGTAG
Homology
[Close]
BLAST of CSPI06G03050 vs. Swiss-Prot
Match: IPT_HUMLU (Adenylate isopentenyltransferase OS=Humulus lupulus PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.6e-86
Identity = 175/301 (58.14%), Postives = 217/301 (72.09%), Query Frame = 1 

Query: 29  DSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHL 88
           + LL+L+GATG+GKS LSIDLAA FP E+INSDKMQ+Y+GLDITTNK+ +  R  VPHHL
Sbjct: 30  EKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 89

Query: 89  LGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDAT-QDVF 148
           LG+ DP  GE++PA FRS A   +S +  R  LP++VGGSNSF+HAL+   FD++   VF
Sbjct: 90  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVF 149

Query: 149 -CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPE----M 208
             GS + VS+E  Y CCFLWVDVS  VL  YL+KRVD+ML+ GM +ELA+FY PE     
Sbjct: 150 EEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHD 209

Query: 209 ADSETRVGLRKAIGVPEFEEYFRRHHP---KGRDYREGDPLQASAFEEAVRKIKENTWQL 268
            DS TR GLRKAIGVPEF+ YF +  P   +G D    D ++  AFEEAVR IKENT  L
Sbjct: 210 EDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGR-DRVRRGAFEEAVRAIKENTCHL 269

Query: 269 TKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQ 321
            KRQ+ KI RL+ AGWDL+R+DAT +  A + S+  EK +EIWE QV+EPSVKIV  F+ 
Sbjct: 270 AKRQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFLD 329

[Close]
BLAST of CSPI06G03050 vs. Swiss-Prot
Match: IPT1_ARATH (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana GN=IPT1 PE=1 SV=2)

HSP 1 Score: 310.5 bits (794), Expect = 2.2e-83
Identity = 165/315 (52.38%), Postives = 217/315 (68.89%), Query Frame = 1 

Query: 10  SFLLSRRPRCPLILSSAPS--------DSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+ S            D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKR 189
           P++ GGSNSFVHAL+A  FD   D F      +S++  Y CCF+WVDVS  VL  YL +R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQ 249
           VDEM+D GM EEL++FYDP  +  ETR G+RKAIGVPEF+ YF+ + P+ +   + D L+
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALR 277

Query: 250 ASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKR-SE 309
            +A+++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+    +EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

[Close]
BLAST of CSPI06G03050 vs. Swiss-Prot
Match: IPT6_ARATH (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana GN=IPT6 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 161/336 (47.92%), Postives = 220/336 (65.48%), Query Frame = 1 

Query: 3   LSLPFSHSFLL---SRRPRCPLILSSAPS--------DSLLLLLGATGSGKSSLSIDLAA 62
           LS P SHS LL   + +   P ++++           D ++L+ G TG+GKS LS+DLA 
Sbjct: 8   LSPPLSHSSLLPTVTTKFGSPRLVTTCMGHAGRKNIKDKVVLITGTTGTGKSRLSVDLAT 67

Query: 63  RF-PSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASA 122
           RF P+EIINSDKMQ+Y+G +I TN +PL  +  VPHHLLG F P  GE++PA FRS A+ 
Sbjct: 68  RFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPAEFRSLATL 127

Query: 123 VISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFC--GSRNRVSTEFLYRCCFLWVD 182
            IS +IS   LP+VVGGSNSF HAL+A  FD   D F    S + + ++  Y+CC LWVD
Sbjct: 128 SISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTICSDLRYKCCILWVD 187

Query: 183 VSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHH 242
           V  PVL ++L  RVD+M++ G+VE+LA+ YDP + DS  R+G+RK IGV EF+ YF R +
Sbjct: 188 VLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEEFDRYF-RVY 247

Query: 243 PKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVA 302
           PK  D    D  + +A+EE V+ +KE T +L K+Q  KI +L R GW++KR+DATAA++A
Sbjct: 248 PKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIKRLDATAAIMA 307

Query: 303 VLASEVAEKRS----EIWETQVVEPSVKIVKGFIQE 321
            L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 308 ELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]
BLAST of CSPI06G03050 vs. Swiss-Prot
Match: IPT4_ARATH (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana GN=IPT4 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.7e-73
Identity = 146/298 (48.99%), Postives = 202/298 (67.79%), Query Frame = 1 

Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS++HAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSET 207
              +  + +E  Y CCF+W+DV   VL  YLS R+D M+  GM EE+A+F+  + A  E 
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKI 267
            +G+ KAIGV EF++Y +  +    D  + DP++  A+E+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-VAEKRSEIWETQVVEPSVKIVKGFIQE 321
            +LR AGWD+K+VDATA    A+ A    E VAE + +IW  +V+EP VKIV+  + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]
BLAST of CSPI06G03050 vs. Swiss-Prot
Match: IPT8_ARATH (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana GN=IPT8 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 7.9e-73
Identity = 141/304 (46.38%), Postives = 199/304 (65.46%), Query Frame = 1 

Query: 17  PRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKL 76
           P   + +  +    +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +TTN++
Sbjct: 31  PMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQM 90

Query: 77  PLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALV 136
            +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF+HAL+
Sbjct: 91  SILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALL 150

Query: 137 AADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAK 196
              FD     F  S   +S+   Y CCFLWVDVS  VL  YLSKRVD+M++ GM EELA 
Sbjct: 151 VDRFDPKTYPF-SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAG 210

Query: 197 FYDPEMADSETRV-GLRKAIGVPEFEEYFRRHHPKGRD-YREGDPLQASAFEEAVRKIKE 256
           FYDP  + S  R  G+ K IG+PEF+ YF  + P+ +    E D  +  A++EAV++IKE
Sbjct: 211 FYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKE 270

Query: 257 NTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIV 316
           NTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+V
Sbjct: 271 NTWRLAKKQIERIMKLKSSGWDIQRLDATPS--------FGRSSREIWDNTVLDESIKVV 325

Query: 317 KGFI 319
           K F+
Sbjct: 331 KRFL 325

[Close]
BLAST of CSPI06G03050 vs. TrEMBL
Match: A0A0A0K943_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.1e-177
Identity = 319/320 (99.69%), Postives = 319/320 (99.69%), Query Frame = 1 

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QA AFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]
BLAST of CSPI06G03050 vs. TrEMBL
Match: A5AJM9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000274 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.0e-90
Identity = 174/296 (58.78%), Postives = 222/296 (75.00%), Query Frame = 1 

Query: 31  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLG 90
           +++++GATG+GKS LSIDLA RFP+EIINSDK+Q+Y GLDITTNK+ +  R+ VPHHLLG
Sbjct: 79  VVVIMGATGTGKSKLSIDLATRFPAEIINSDKIQIYSGLDITTNKIQMHERQGVPHHLLG 138

Query: 91  DFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGS 150
           DFD    E++P+ FRS A+A IS + SR  LP++ GGSNSF+HAL+   FD+  D F GS
Sbjct: 139 DFDSSHAEITPSQFRSVAAAAISDISSRRKLPVLAGGSNSFIHALLVDRFDSESDPFNGS 198

Query: 151 RNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVG 210
            + VSTE  YRCCFLWVDVSF VL  YLSKRVDEML  GM+EELAKFYDP+  +S  + G
Sbjct: 199 -DSVSTELRYRCCFLWVDVSFAVLSDYLSKRVDEMLGSGMLEELAKFYDPDEDESRPKTG 258

Query: 211 LRKAIGVPEFEEYFRRHHP------KGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQL 270
           LRKAIGVPEF+ +FR++ P       G   ++ D  +  +FEEAV+ IK+NT  L K+Q+
Sbjct: 259 LRKAIGVPEFDRHFRKYPPVDQGIIAGNPKKKKDDPEMESFEEAVKAIKDNTCHLAKKQI 318

Query: 271 WKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
            KI R+R AGWDLKR+DAT A   +L+S+  +K SEIWE QVVEPSVK V+ F++E
Sbjct: 319 EKILRMRGAGWDLKRLDATEAFRVLLSSDSGKKSSEIWEKQVVEPSVKFVRRFLEE 373

[Close]
BLAST of CSPI06G03050 vs. TrEMBL
Match: F6HEW3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g03640 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-90
Identity = 173/290 (59.66%), Postives = 219/290 (75.52%), Query Frame = 1 

Query: 31  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLG 90
           +++++GATG+GKS LSIDLA RFP+EIINSDK+Q+Y GLDITTNK+ +  R+ VPHHLLG
Sbjct: 50  VVVIMGATGTGKSKLSIDLATRFPAEIINSDKIQIYSGLDITTNKIQMHERQGVPHHLLG 109

Query: 91  DFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGS 150
           DFD    E++P+ FRS A+A IS + SR  LP++ GGSNSF+HAL+   FD+  D F GS
Sbjct: 110 DFDSSHAEITPSQFRSVAAAAISDISSRRKLPVLAGGSNSFIHALLVDRFDSESDPFNGS 169

Query: 151 RNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVG 210
            + VSTE  YRCCFLWVDVSF VL  YLSKRVDEML  GM+EELAKFYDP+  +S  + G
Sbjct: 170 -DSVSTELRYRCCFLWVDVSFAVLSDYLSKRVDEMLGSGMLEELAKFYDPDEDESRPKTG 229

Query: 211 LRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRL 270
           LRKAIGVPEF+ +FR++ P        D  +  +FEEAV+ IK+NT  L K+Q+ KI R+
Sbjct: 230 LRKAIGVPEFDRHFRKYPP--------DDPEMESFEEAVKAIKDNTCHLAKKQIEKILRM 289

Query: 271 RRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
           R AGWDLKR+DAT A   +L+S+  +K SEIWE QVVEPSVK V+ F++E
Sbjct: 290 RGAGWDLKRLDATEAFRVLLSSDSGKKSSEIWEKQVVEPSVKFVRRFLEE 330

[Close]
BLAST of CSPI06G03050 vs. TrEMBL
Match: V7BDD3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G028100g PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.2e-89
Identity = 176/294 (59.86%), Postives = 218/294 (74.15%), Query Frame = 1 

Query: 29  DSLLLLLGATGSGKSSLSIDLAARFP-SEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 88
           D +++++GATGSGKS LSIDLA  FP SEIINSDKMQ+YRGLDITTNK+P + RRAVPHH
Sbjct: 32  DKVVVIMGATGSGKSRLSIDLATLFPVSEIINSDKMQVYRGLDITTNKIPHAQRRAVPHH 91

Query: 89  LLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVF 148
           LLGD DP   E SPA FR  A  +I  +  R +LP+VVGGSNSFVHAL+  DF    +VF
Sbjct: 92  LLGDVDPSLPEFSPADFRRRAGHLIEDINRRDSLPIVVGGSNSFVHALLVQDFQPDSNVF 151

Query: 149 CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSET 208
                 +S+E  YRCCFLWVD++FPVL +YL  RVD+ML+ GMV+ELA+F+DPE A  E+
Sbjct: 152 RQGEELISSELRYRCCFLWVDIAFPVLSQYLCDRVDDMLESGMVDELAQFFDPE-ASRES 211

Query: 209 RVGLRKAIGVPEFEEYFRRHHPKGRDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWK 268
             G+RKAIGVPEF+ YF ++ P       G DPL+  A+EEAVR IKENT +L +RQ+ K
Sbjct: 212 ESGIRKAIGVPEFDRYFNKYPPWAGPAGPGQDPLRERAYEEAVRAIKENTCELAERQMEK 271

Query: 269 IGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
           I RL+RAGWDL+R+DAT A   VL S  +   S++WE QV+EPSVKIVK F+ E
Sbjct: 272 IERLKRAGWDLRRIDATEAFRMVLTSG-SNHGSDVWERQVLEPSVKIVKRFLME 323

[Close]
BLAST of CSPI06G03050 vs. TrEMBL
Match: A0A059BGB7_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_G02313 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.2e-89
Identity = 176/307 (57.33%), Postives = 219/307 (71.34%), Query Frame = 1 

Query: 31  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLG 90
           +++++GATG GKS LSIDLA+ FPSE+INSDKMQ+YRGLDITTNK+  + RR VPHHLLG
Sbjct: 1   VVVIMGATGCGKSGLSIDLASVFPSEVINSDKMQVYRGLDITTNKITPAERRGVPHHLLG 60

Query: 91  DFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGS 150
            FDP  GE+SP+ FRS   + ISG+ SRGNLPL+ GGSNS +HAL+   FD   DVF G 
Sbjct: 61  SFDPDDGEMSPSMFRSLGQSAISGIASRGNLPLLAGGSNSLIHALLVERFDPEVDVFGGL 120

Query: 151 RNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVG 210
            +  S E  Y CCFLWVDVS PVL+ YLSKRVD+MLD GM EELA++YDP   D   R G
Sbjct: 121 TSPASLELRYNCCFLWVDVSPPVLEEYLSKRVDDMLDMGMFEELARYYDPRRTDPGGRTG 180

Query: 211 LRKAIGVPEFEEYFRRHHP------------KGRDYREGDPLQA--SAFEEAVRKIKENT 270
           LRKAIGVPEF+ YF++H P            +  + REGD  +A   A+EEAV +IKENT
Sbjct: 181 LRKAIGVPEFDRYFKKHPPQRWAGQGQGDARRPEEGREGDDDRAREGAYEEAVWEIKENT 240

Query: 271 WQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEV---AEKRSEIWETQVVEPSVKI 321
            QL KRQ+ KI RLR  GW+L+++DAT   +  +A++      +R ++WE QVV PSVKI
Sbjct: 241 RQLAKRQIEKIERLRECGWELRKLDATETFLLAMATDEGGGGGRRRDVWERQVVGPSVKI 300

[Close]
BLAST of CSPI06G03050 vs. TAIR10
Match: AT1G68460.1 (AT1G68460.1 isopentenyltransferase 1)

HSP 1 Score: 310.5 bits (794), Expect = 1.2e-84
Identity = 165/315 (52.38%), Postives = 217/315 (68.89%), Query Frame = 1 

Query: 10  SFLLSRRPRCPLILSSAPS--------DSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+ S            D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKR 189
           P++ GGSNSFVHAL+A  FD   D F      +S++  Y CCF+WVDVS  VL  YL +R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQ 249
           VDEM+D GM EEL++FYDP  +  ETR G+RKAIGVPEF+ YF+ + P+ +   + D L+
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALR 277

Query: 250 ASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKR-SE 309
            +A+++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+    +EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

[Close]
BLAST of CSPI06G03050 vs. TAIR10
Match: AT1G25410.1 (AT1G25410.1 isopentenyltransferase 6)

HSP 1 Score: 278.1 bits (710), Expect = 6.8e-75
Identity = 161/336 (47.92%), Postives = 220/336 (65.48%), Query Frame = 1 

Query: 3   LSLPFSHSFLL---SRRPRCPLILSSAPS--------DSLLLLLGATGSGKSSLSIDLAA 62
           LS P SHS LL   + +   P ++++           D ++L+ G TG+GKS LS+DLA 
Sbjct: 8   LSPPLSHSSLLPTVTTKFGSPRLVTTCMGHAGRKNIKDKVVLITGTTGTGKSRLSVDLAT 67

Query: 63  RF-PSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASA 122
           RF P+EIINSDKMQ+Y+G +I TN +PL  +  VPHHLLG F P  GE++PA FRS A+ 
Sbjct: 68  RFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPAEFRSLATL 127

Query: 123 VISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFC--GSRNRVSTEFLYRCCFLWVD 182
            IS +IS   LP+VVGGSNSF HAL+A  FD   D F    S + + ++  Y+CC LWVD
Sbjct: 128 SISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSPGSSLSTICSDLRYKCCILWVD 187

Query: 183 VSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHH 242
           V  PVL ++L  RVD+M++ G+VE+LA+ YDP + DS  R+G+RK IGV EF+ YF R +
Sbjct: 188 VLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEEFDRYF-RVY 247

Query: 243 PKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVA 302
           PK  D    D  + +A+EE V+ +KE T +L K+Q  KI +L R GW++KR+DATAA++A
Sbjct: 248 PKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIKRLDATAAIMA 307

Query: 303 VLASEVAEKRS----EIWETQVVEPSVKIVKGFIQE 321
            L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 308 ELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]
BLAST of CSPI06G03050 vs. TAIR10
Match: AT4G24650.1 (AT4G24650.1 isopentenyltransferase 4)

HSP 1 Score: 276.9 bits (707), Expect = 1.5e-74
Identity = 146/298 (48.99%), Postives = 202/298 (67.79%), Query Frame = 1 

Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS++HAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 CGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSET 207
              +  + +E  Y CCF+W+DV   VL  YLS R+D M+  GM EE+A+F+  + A  E 
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKI 267
            +G+ KAIGV EF++Y +  +    D  + DP++  A+E+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-VAEKRSEIWETQVVEPSVKIVKGFIQE 321
            +LR AGWD+K+VDATA    A+ A    E VAE + +IW  +V+EP VKIV+  + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]
BLAST of CSPI06G03050 vs. TAIR10
Match: AT3G19160.1 (AT3G19160.1 ATP/ADP isopentenyltransferases)

HSP 1 Score: 275.4 bits (703), Expect = 4.4e-74
Identity = 141/304 (46.38%), Postives = 199/304 (65.46%), Query Frame = 1 

Query: 17  PRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKL 76
           P   + +  +    +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +TTN++
Sbjct: 31  PMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQM 90

Query: 77  PLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALV 136
            +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF+HAL+
Sbjct: 91  SILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALL 150

Query: 137 AADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAK 196
              FD     F  S   +S+   Y CCFLWVDVS  VL  YLSKRVD+M++ GM EELA 
Sbjct: 151 VDRFDPKTYPF-SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAG 210

Query: 197 FYDPEMADSETRV-GLRKAIGVPEFEEYFRRHHPKGRD-YREGDPLQASAFEEAVRKIKE 256
           FYDP  + S  R  G+ K IG+PEF+ YF  + P+ +    E D  +  A++EAV++IKE
Sbjct: 211 FYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKE 270

Query: 257 NTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIV 316
           NTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+V
Sbjct: 271 NTWRLAKKQIERIMKLKSSGWDIQRLDATPS--------FGRSSREIWDNTVLDESIKVV 325

Query: 317 KGFI 319
           K F+
Sbjct: 331 KRFL 325

[Close]
BLAST of CSPI06G03050 vs. TAIR10
Match: AT3G23630.1 (AT3G23630.1 isopentenyltransferase 7)

HSP 1 Score: 211.5 bits (537), Expect = 7.9e-55
Identity = 128/296 (43.24%), Postives = 172/296 (58.11%), Query Frame = 1 

Query: 27  PSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPH 86
           P + ++ ++GATGSGKS L+IDLA RF  EIINSDK+QLY+GLD+ TNK+     R VPH
Sbjct: 32  PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPH 91

Query: 87  HLLGDFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDV 146
           HLLG FD  AG ++   +   AS  IS + +   LP+V GGSNS++ ALV          
Sbjct: 92  HLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALV---------- 151

Query: 147 FCGSRNRVSTEFL---YRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMA 206
                   S+ FL   Y CCF+WVDVS PVL  ++SKRVD M++ G++EE+ + ++P+  
Sbjct: 152 ------NHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPK-- 211

Query: 207 DSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQ 266
            +   VG+R+AIGVPE  EY R      R        ++   + AV+ IK+NT  L  RQ
Sbjct: 212 -ANYSVGIRRAIGVPELHEYLRNESLVDR------ATKSKMLDVAVKNIKKNTEILACRQ 271

Query: 267 LWKIGRLRRAGW--DLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGF 318
           L KI RL +  W   + RVDAT     V      E++ E WE  V  PS +IV  F
Sbjct: 272 LKKIQRLHKK-WKMSMHRVDAT----EVFLKRNVEEQDEAWENLVARPSERIVDKF 297

[Close]
BLAST of CSPI06G03050 vs. NCBI nr
Match: gi|449466400|ref|XP_004150914.1| (PREDICTED: adenylate isopentenyltransferase-like [Cucumis sativus])

HSP 1 Score: 630.6 bits (1625), Expect = 1.5e-177
Identity = 319/320 (99.69%), Postives = 319/320 (99.69%), Query Frame = 1 

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QA AFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]
BLAST of CSPI06G03050 vs. NCBI nr
Match: gi|659089679|ref|XP_008445640.1| (PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo])

HSP 1 Score: 614.8 bits (1584), Expect = 8.8e-173
Identity = 309/320 (96.56%), Postives = 314/320 (98.12%), Query Frame = 1 

Query: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPL+LSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGVISRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180
           LPLVVGGSNSF+HALVAADFD TQDVFCGSRNRVS EF YRCCFLWVDVSFPVLKRYLSK
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240
           RVDEMLDGGM+EELA+FYDPEMAD ETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300
           QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKGFIQE 321
           IWETQVVEPSVKIVKGFIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]
BLAST of CSPI06G03050 vs. NCBI nr
Match: gi|147778591|emb|CAN73470.1| (hypothetical protein VITISV_000274 [Vitis vinifera])

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-90
Identity = 174/296 (58.78%), Postives = 222/296 (75.00%), Query Frame = 1 

Query: 31  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLG 90
           +++++GATG+GKS LSIDLA RFP+EIINSDK+Q+Y GLDITTNK+ +  R+ VPHHLLG
Sbjct: 79  VVVIMGATGTGKSKLSIDLATRFPAEIINSDKIQIYSGLDITTNKIQMHERQGVPHHLLG 138

Query: 91  DFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGS 150
           DFD    E++P+ FRS A+A IS + SR  LP++ GGSNSF+HAL+   FD+  D F GS
Sbjct: 139 DFDSSHAEITPSQFRSVAAAAISDISSRRKLPVLAGGSNSFIHALLVDRFDSESDPFNGS 198

Query: 151 RNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVG 210
            + VSTE  YRCCFLWVDVSF VL  YLSKRVDEML  GM+EELAKFYDP+  +S  + G
Sbjct: 199 -DSVSTELRYRCCFLWVDVSFAVLSDYLSKRVDEMLGSGMLEELAKFYDPDEDESRPKTG 258

Query: 211 LRKAIGVPEFEEYFRRHHP------KGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQL 270
           LRKAIGVPEF+ +FR++ P       G   ++ D  +  +FEEAV+ IK+NT  L K+Q+
Sbjct: 259 LRKAIGVPEFDRHFRKYPPVDQGIIAGNPKKKKDDPEMESFEEAVKAIKDNTCHLAKKQI 318

Query: 271 WKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
            KI R+R AGWDLKR+DAT A   +L+S+  +K SEIWE QVVEPSVK V+ F++E
Sbjct: 319 EKILRMRGAGWDLKRLDATEAFRVLLSSDSGKKSSEIWEKQVVEPSVKFVRRFLEE 373

[Close]
BLAST of CSPI06G03050 vs. NCBI nr
Match: gi|359472688|ref|XP_002279371.2| (PREDICTED: adenylate isopentenyltransferase [Vitis vinifera])

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-90
Identity = 174/296 (58.78%), Postives = 222/296 (75.00%), Query Frame = 1 

Query: 31  LLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLG 90
           +++++GATG+GKS LSIDLA RFP+EIINSDK+Q+Y GLDITTNK+ +  R+ VPHHLLG
Sbjct: 87  VVVIMGATGTGKSKLSIDLATRFPAEIINSDKIQIYSGLDITTNKIQMHERQGVPHHLLG 146

Query: 91  DFDPLAGEVSPAAFRSHASAVISGVISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGS 150
           DFD    E++P+ FRS A+A IS + SR  LP++ GGSNSF+HAL+   FD+  D F GS
Sbjct: 147 DFDSSHAEITPSQFRSVAAAAISDISSRRKLPVLAGGSNSFIHALLVDRFDSESDPFNGS 206

Query: 151 RNRVSTEFLYRCCFLWVDVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVG 210
            + VSTE  YRCCFLWVDVSF VL  YLSKRVDEML  GM+EELAKFYDP+  +S  + G
Sbjct: 207 -DSVSTELRYRCCFLWVDVSFAVLSDYLSKRVDEMLGSGMLEELAKFYDPDEDESRPKTG 266

Query: 211 LRKAIGVPEFEEYFRRHHP------KGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQL 270
           LRKAIGVPEF+ +FR++ P       G   ++ D  +  +FEEAV+ IK+NT  L K+Q+
Sbjct: 267 LRKAIGVPEFDRHFRKYPPVDQGIIAGNPKKKKDDPEMESFEEAVKAIKDNTCHLAKKQI 326

Query: 271 WKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSEIWETQVVEPSVKIVKGFIQE 321
            KI R+R AGWDLKR+DAT A   +L+S+  +K SEIWE QVVEPSVK V+ F++E
Sbjct: 327 EKILRMRGAGWDLKRLDATEAFRVLLSSDSGKKSSEIWEKQVVEPSVKFVRRFLEE 381

[Close]
BLAST of CSPI06G03050 vs. NCBI nr
Match: gi|828305886|ref|XP_012570225.1| (PREDICTED: adenylate isopentenyltransferase [Cicer arietinum])

HSP 1 Score: 340.5 bits (872), Expect = 3.2e-90
Identity = 185/336 (55.06%), Postives = 237/336 (70.54%), Query Frame = 1 

Query: 1   MTLSLPFSHSFLLSR-RPRCPLILSSAP----SDSLLLLLGATGSGKSSLSIDLAARFP- 60
           +TL +P S S   SR +PR P + S+A      D +++++GATGSGKS LS++LA  FP 
Sbjct: 27  LTLFIPSSSSSSSSRTKPRWPRMDSAASRHRRKDKVVVIMGATGSGKSRLSVELATLFPF 86

Query: 61  SEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISG 120
           SEIINSDKMQ+YRGLDITTNK+P   R  VPHHLLGD D   GE SP+ FR HA  +IS 
Sbjct: 87  SEIINSDKMQVYRGLDITTNKIPFHQRNGVPHHLLGDVDHSHGEFSPSDFRRHAGEIISD 146

Query: 121 VISRGNLPLVVGGSNSFVHALVAADFDATQDVF-------CGSRNRVSTEFLYRCCFLWV 180
           + SR  LP++VGGSNSF+HAL+   FD   +VF         S + +S++  Y+CCFLW+
Sbjct: 147 ITSRRKLPIIVGGSNSFIHALLVERFDPELNVFDKSSSSSSSSLSSISSDLRYKCCFLWM 206

Query: 181 DVSFPVLKRYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRH 240
           D+SFPVL  YL KRVDEM D GMV+ELA+F++P   DSE R GLRKAIGVPEF+ +F ++
Sbjct: 207 DISFPVLSEYLLKRVDEMFDSGMVDELAQFFEP---DSENRTGLRKAIGVPEFDRFFNKY 266

Query: 241 HPKGRDYREGDPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVV 300
            P  +     +P++  A+EEAVR IK+NT QL KRQ+ KI RL+RAGWDL+R+DAT A  
Sbjct: 267 PPADKLEEGENPMREGAYEEAVRAIKDNTCQLAKRQIGKILRLKRAGWDLRRIDATEAFR 326

Query: 301 AVLASEV---AEKRSEIWETQVVEPSVKIVKGFIQE 321
           AVL SE    A   S++W+ QV+EPSVKIVK F+ E
Sbjct: 327 AVLTSESRGGAGVFSDLWKNQVLEPSVKIVKRFLME 359

The following BLAST results are available for this feature:
BLAST of CSPI06G03050 vs. Swiss-Prot
Match NameE-valueIdentityDescription
IPT_HUMLU1.6e-8658.14Adenylate isopentenyltransferase OS=Humulus lupulus PE=1 SV=1[more]
IPT1_ARATH2.2e-8352.38Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana GN=IPT... [more]
IPT6_ARATH1.2e-7347.92Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana GN=IPT... [more]
IPT4_ARATH2.7e-7348.99Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana GN=IPT4 PE=1 SV=1[more]
IPT8_ARATH7.9e-7346.38Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana GN=IPT... [more]
BLAST of CSPI06G03050 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K943_CUCSA1.1e-17799.69Uncharacterized protein OS=Cucumis sativus GN=Csa_6G030440 PE=4 SV=1[more]
A5AJM9_VITVI1.0e-9058.78Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000274 PE=4 SV=1[more]
F6HEW3_VITVI2.2e-9059.66Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g03640 PE=4 SV=... [more]
V7BDD3_PHAVU3.2e-8959.86Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G028100g PE=4 SV=1[more]
A0A059BGB7_EUCGR3.2e-8957.33Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_G02313 PE=4 ... [more]
BLAST of CSPI06G03050 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G68460.11.2e-8452.38 isopentenyltransferase 1[more]
AT1G25410.16.8e-7547.92 isopentenyltransferase 6[more]
AT4G24650.11.5e-7448.99 isopentenyltransferase 4[more]
AT3G19160.14.4e-7446.38 ATP/ADP isopentenyltransferases[more]
AT3G23630.17.9e-5543.24 isopentenyltransferase 7[more]
BLAST of CSPI06G03050 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449466400|ref|XP_004150914.1|1.5e-17799.69PREDICTED: adenylate isopentenyltransferase-like [Cucumis sativus][more]
gi|659089679|ref|XP_008445640.1|8.8e-17396.56PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo][more]
gi|147778591|emb|CAN73470.1|1.4e-9058.78hypothetical protein VITISV_000274 [Vitis vinifera][more]
gi|359472688|ref|XP_002279371.2|1.4e-9058.78PREDICTED: adenylate isopentenyltransferase [Vitis vinifera][more]
gi|828305886|ref|XP_012570225.1|3.2e-9055.06PREDICTED: adenylate isopentenyltransferase [Cicer arietinum][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018022IPT
IPR027417P-loop_NTPase
Vocabulary: Biological Process
TermDefinition
GO:0008033tRNA processing
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034265 isopentenyl adenine biosynthetic process
biological_process GO:0008033 tRNA processing
cellular_component GO:0005575 cellular_component
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G03050.1CSPI06G03050.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018022tRNA dimethylallyltransferasePFAMPF01715IPPTcoord: 64..137
score: 1.6E-15coord: 161..264
score: 5.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 29..144
score: 3.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 31..205
score: 2.47
NoneNo IPR availableGENE3DG3DSA:1.10.287.890coord: 173..264
score: 6.9
NoneNo IPR availablePANTHERPTHR11088TRNA DELTA 2 -ISOPENTENYLPYROPHOSPHATE TRANSFERASE-RELATEDcoord: 17..320
score: 6.8E
NoneNo IPR availablePANTHERPTHR11088:SF27ADENYLATE ISOPENTENYLTRANSFERASE 1, CHLOROPLASTIC-RELATEDcoord: 17..320
score: 6.8E