Lsi07G013420 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi07G013420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCalcium-binding EF-hand family protein
Locationchr07 : 19587340 .. 19589151 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAAAGATCTGAGCGATGATCAGAAATCCTCCATGAGAGAGGCCTTTACCCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTCACTGCTCCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCCTGAGCCCTTCGATCGCCAGCTTCGCGATGCCTTTAAGGTTCTCGATAAGGACAACACCGGCTATGTCCTCGTGTCGGAGCTCCGACACATTTTGACGAGTATAGGTGAGAAGCTTGAGCCGTCGGAGTTCGATGAGTGGATTCGAGAAGTTGAGGTTGGATCCGATGGAAGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGAGGAGGGAAGTAATGGGAAATTGATTTTCTGTGCTTTACTGGAATGGTTTCTGAAGTAATTTGTGGTGATCTTTGTTGTTGTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTACTTTTTCCCCTTTCCAATTTCTGTTAATTGGTTTCAGATTACAAGACTTGGTTGGATTTTGGTGGGATGGATGCGGGTGATGTTCGCATACTTTCCCAAGTAATGCTAATATTATTTGGAAAGATTTAGGATTATGATGAGATATTTCTGGATGGATTCGAGGTGTATTGAAAACTGTGGTTTTCTGGGTAACTTAGAATTTGGTGTTCTTTATGTGTTTCTTAGCTTGAAGTCCAAAAAAATGAGTAGCTTCTCCCACGATTTATTTTCTTGCAATGGTTGTTTCTTGGGGGTCTTTAAGTTAGAGAGATTGAGATGGTGTCTTTATGTTCACCATGGCTTGTTTAGTTTGTAAGATTTGAAAGATTAATTAATCTTCTTCTTGTTTCATTTGGTTTAGCCTTATTTTTCCATATTATCCTCATGAGATTCAAGACCTGACGTGCATATGTTATATCTTTAAATCCTCCTGATATATTTTAGGGGTTTTGGGACTAATTATATTGTATGTGTTTAAGTTTAAGATGTGTAAAGGATTCATTGCTATGATGTAAGAGTATCGAATGCATTGAATTGAGTAAAAAAATTTGCATCAAAAGGTTGGTTCTAGACTCCATTTTTATTCTATATGATTTTGTATTTTCATAAAGGAAAATTAGTTTACTCAAAGTAGATCCCTCTGAATTTGTTGTAGAAGAAGAGATCTCCTATACGTGGGCAGGAGCGCAGATGCTTTTAAAACTTAAACCTTTCTGTATTTGAGATACTATACCTTCTGTGAGGTTTTGAGATGATAAAGTAAAGTTTTTAGCTGTAAGGCTGTGAAGCTGCAGGAAGTCAGTTGTAAACCTTCTGTGTTCTGCTTTACCATTTAGCTTTTGAACATTCTTTTTTGTTATGAATTCTGAGGAAACATTGTAGTTTGTAAGTTTTGGTACTGATTTACAACAGGACCATGGTTTGGCTCTCTGTTGGAAATCTGTGCATTCCTTGTATTTATAATGAACCAGATTTTGAAGGTTCTAAGTTGTCTTCATGGGATACCGCGGTGAGGTTTGTGGTGCAGTTTAACATGTAG

mRNA sequence

ATGGGCAAAGATCTGAGCGATGATCAGAAATCCTCCATGAGAGAGGCCTTTACCCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTCACTGCTCCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCCTGAGCCCTTCGATCGCCAGCTTCGCGATGCCTTTAAGGTTCTCGATAAGGACAACACCGGCTATGTCCTCGTGTCGGAGCTCCGACACATTTTGACGAGTATAGGTGAGAAGCTTGAGCCGTCGGAGTTCGATGAGTGGATTCGAGAAGTTGAGGTTGGATCCGATGGAAGAATTCGGACCATGGTTTGGCTCTCTGTTGGAAATCTGTGCATTCCTTGTATTTATAATGAACCAGATTTTGAAGGTTCTAAGTTGTCTTCATGGGATACCGCGGTGAGGTTTGTGGTGCAGTTTAACATGTAG

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCGATGATCAGAAATCCTCCATGAGAGAGGCCTTTACCCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTCACTGCTCCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCCTGAGCCCTTCGATCGCCAGCTTCGCGATGCCTTTAAGGTTCTCGATAAGGACAACACCGGCTATGTCCTCGTGTCGGAGCTCCGACACATTTTGACGAGTATAGGTGAGAAGCTTGAGCCGTCGGAGTTCGATGAGTGGATTCGAGAAGTTGAGGTTGGATCCGATGGAAGAATTCGGACCATGGTTTGGCTCTCTGTTGGAAATCTGTGCATTCCTTGTATTTATAATGAACCAGATTTTGAAGGTTCTAAGTTGTCTTCATGGGATACCGCGGTGAGGTTTGTGGTGCAGTTTAACATGTAG

Protein sequence

MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFDEWIREVEVGSDGRIRTMVWLSVGNLCIPCIYNEPDFEGSKLSSWDTAVRFVVQFNM
Homology
[Close]
BLAST of Lsi07G013420 vs. Swiss-Prot
Match: CML13_ARATH (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana GN=CML13 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 6.5e-61
Identity = 113/137 (82.48%), Postives = 130/137 (94.89%), Query Frame = 1 

Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSDDQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGSDGRIR 137
           FDEWI+EV+VGSDG+IR
Sbjct: 121 FDEWIKEVDVGSDGKIR 137

[Close]
BLAST of Lsi07G013420 vs. Swiss-Prot
Match: CML14_ARATH (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana GN=CML14 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.3e-58
Identity = 108/137 (78.83%), Postives = 128/137 (93.43%), Query Frame = 1 

Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS+DQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGSDGRIR 137
           FDEWI+EV+VGSDG+IR
Sbjct: 121 FDEWIKEVDVGSDGKIR 137

[Close]
BLAST of Lsi07G013420 vs. Swiss-Prot
Match: CML7_ORYSJ (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica GN=CML7 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 5.3e-55
Identity = 102/135 (75.56%), Postives = 120/135 (88.89%), Query Frame = 1 

Query: 2   GKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAP 61
           GK+LS++Q +SMREAF+LFDTDGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LTAP
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFL++M  H++PEPFDR LRDAF+VLDKD +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVEVGSDGRIR 137
           EWIREV+V  DG IR
Sbjct: 123 EWIREVDVAPDGTIR 137

[Close]
BLAST of Lsi07G013420 vs. Swiss-Prot
Match: CALM6_ARATH (Calmodulin-6 OS=Arabidopsis thaliana GN=CAM6 PE=1 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 7.0e-31
Identity = 66/137 (48.18%), Postives = 92/137 (67.15%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGSDGRI 136
           E DE IRE +V  DG+I
Sbjct: 121 EVDEMIREADVDGDGQI 137

[Close]
BLAST of Lsi07G013420 vs. Swiss-Prot
Match: CALM_EUPCH (Calmodulin OS=Euphorbia characias PE=2 SV=4)

HSP 1 Score: 134.8 bits (338), Expect = 9.2e-31
Identity = 66/137 (48.18%), Postives = 92/137 (67.15%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRI 136
           E DE IRE +V  DG+I
Sbjct: 121 EVDEMIREADVDGDGQI 137

[Close]
BLAST of Lsi07G013420 vs. TrEMBL
Match: A0A0A0L2F2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G036390 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.3e-68
Identity = 132/136 (97.06%), Postives = 134/136 (98.53%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTGYV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREVEVGSDG IR
Sbjct: 121 DEWIREVEVGSDGSIR 136

[Close]
BLAST of Lsi07G013420 vs. TrEMBL
Match: I1LGL7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_11G030100 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 5.7e-64
Identity = 121/136 (88.97%), Postives = 133/136 (97.79%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSD+Q SSM+EAF+LFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAI+AEENLTA
Sbjct: 1   MGKDLSDEQVSSMKEAFSLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIVAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFL++M+KHMKPEPFDRQLRDAFKVLDKD+TG+V VSELRHILT+IGEKLEPSEF
Sbjct: 61  PFDFPRFLDLMAKHMKPEPFDRQLRDAFKVLDKDSTGFVAVSELRHILTNIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VGSDG+IR
Sbjct: 121 DEWIREVDVGSDGKIR 136

[Close]
BLAST of Lsi07G013420 vs. TrEMBL
Match: A0A022QCM8_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a015755mg PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.7e-63
Identity = 119/136 (87.50%), Postives = 132/136 (97.06%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSM+EAFTLFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+II+EE L A
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIISEEKLNA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RF+++MSKH+KPEPFDRQLRDAFKVLDKD TGYV+VS+LRHILTSIGEKLEP+EF
Sbjct: 61  PFDFARFIDLMSKHLKPEPFDRQLRDAFKVLDKDGTGYVVVSDLRHILTSIGEKLEPAEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VGSDG+IR
Sbjct: 121 DEWIREVDVGSDGKIR 136

[Close]
BLAST of Lsi07G013420 vs. TrEMBL
Match: A0A067FK09_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g032081mg PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.2e-63
Identity = 120/136 (88.24%), Postives = 132/136 (97.06%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQ SSM+EAFTLFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+II+EE LTA
Sbjct: 1   MGKDLSDDQVSSMKEAFTLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIISEEKLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFL++M+KHMKPEPFDRQLRDAFKVLDKDNTG+V VS+LRHILTSIGEKLEPSEF
Sbjct: 61  PFDFPRFLDLMAKHMKPEPFDRQLRDAFKVLDKDNTGFVSVSDLRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VGSDG+I+
Sbjct: 121 DEWIREVDVGSDGKIK 136

[Close]
BLAST of Lsi07G013420 vs. TrEMBL
Match: A0A0D2Q0R6_GOSRA (Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_008G239400 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.2e-63
Identity = 121/136 (88.97%), Postives = 131/136 (96.32%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSD+Q SSM+EAFTLFDTDGDG+IAPSELGILMRSLGGNPTQAQLKAIIAEE LTA
Sbjct: 60  MGKDLSDEQVSSMKEAFTLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKAIIAEEKLTA 119

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFL++MSKHMKPEPFDRQLRDAFKVLDKDNTG+V V++LRHILTSIGEKLEPSEF
Sbjct: 120 PFDFPRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVSVADLRHILTSIGEKLEPSEF 179

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VG DG+IR
Sbjct: 180 DEWIREVDVGPDGKIR 195

[Close]
BLAST of Lsi07G013420 vs. TAIR10
Match: AT1G12310.1 (AT1G12310.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 235.0 bits (598), Expect = 3.7e-62
Identity = 113/137 (82.48%), Postives = 130/137 (94.89%), Query Frame = 1 

Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSDDQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGSDGRIR 137
           FDEWI+EV+VGSDG+IR
Sbjct: 121 FDEWIKEVDVGSDGKIR 137

[Close]
BLAST of Lsi07G013420 vs. TAIR10
Match: AT1G62820.1 (AT1G62820.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 226.5 bits (576), Expect = 1.3e-59
Identity = 108/137 (78.83%), Postives = 128/137 (93.43%), Query Frame = 1 

Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS+DQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGSDGRIR 137
           FDEWI+EV+VGSDG+IR
Sbjct: 121 FDEWIKEVDVGSDGKIR 137

[Close]
BLAST of Lsi07G013420 vs. TAIR10
Match: AT5G21274.1 (AT5G21274.1 calmodulin 6)

HSP 1 Score: 135.2 bits (339), Expect = 4.0e-32
Identity = 66/137 (48.18%), Postives = 92/137 (67.15%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGSDGRI 136
           E DE IRE +V  DG+I
Sbjct: 121 EVDEMIREADVDGDGQI 137

[Close]
BLAST of Lsi07G013420 vs. TAIR10
Match: AT3G43810.1 (AT3G43810.1 calmodulin 7)

HSP 1 Score: 134.8 bits (338), Expect = 5.2e-32
Identity = 66/137 (48.18%), Postives = 92/137 (67.15%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRI 136
           E DE IRE +V  DG+I
Sbjct: 121 EVDEMIREADVDGDGQI 137

[Close]
BLAST of Lsi07G013420 vs. TAIR10
Match: AT3G56800.1 (AT3G56800.1 calmodulin 3)

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-31
Identity = 65/137 (47.45%), Postives = 92/137 (67.15%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRI 136
           E DE I+E +V  DG+I
Sbjct: 121 EVDEMIKEADVDGDGQI 137

[Close]
BLAST of Lsi07G013420 vs. NCBI nr
Match: gi|449460417|ref|XP_004147942.1| (PREDICTED: probable calcium-binding protein CML13 [Cucumis sativus])

HSP 1 Score: 267.3 bits (682), Expect = 1.9e-68
Identity = 132/136 (97.06%), Postives = 134/136 (98.53%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTGYV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREVEVGSDG IR
Sbjct: 121 DEWIREVEVGSDGSIR 136

[Close]
BLAST of Lsi07G013420 vs. NCBI nr
Match: gi|659096063|ref|XP_008448907.1| (PREDICTED: probable calcium-binding protein CML13 [Cucumis melo])

HSP 1 Score: 265.8 bits (678), Expect = 5.5e-68
Identity = 131/136 (96.32%), Postives = 134/136 (98.53%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREVEVGSDG IR
Sbjct: 121 DEWIREVEVGSDGSIR 136

[Close]
BLAST of Lsi07G013420 vs. NCBI nr
Match: gi|502160156|ref|XP_004511652.1| (PREDICTED: probable calcium-binding protein CML13 [Cicer arietinum])

HSP 1 Score: 253.1 bits (645), Expect = 3.7e-64
Identity = 121/136 (88.97%), Postives = 134/136 (98.53%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQ SSM+EAFTLFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+I+AEENLTA
Sbjct: 1   MGKDLSDDQISSMKEAFTLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIVAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF+RFL++MSKHMKPEPFDRQLRDAFKVLDKD+TG+V V+ELRHILTSIGEKLEP+EF
Sbjct: 61  PFDFSRFLDLMSKHMKPEPFDRQLRDAFKVLDKDSTGFVSVTELRHILTSIGEKLEPAEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VGSDG+IR
Sbjct: 121 DEWIREVDVGSDGKIR 136

[Close]
BLAST of Lsi07G013420 vs. NCBI nr
Match: gi|947079238|gb|KRH28027.1| (hypothetical protein GLYMA_11G030100 [Glycine max])

HSP 1 Score: 251.9 bits (642), Expect = 8.2e-64
Identity = 121/136 (88.97%), Postives = 133/136 (97.79%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSD+Q SSM+EAF+LFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAI+AEENLTA
Sbjct: 1   MGKDLSDEQVSSMKEAFSLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIVAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFL++M+KHMKPEPFDRQLRDAFKVLDKD+TG+V VSELRHILT+IGEKLEPSEF
Sbjct: 61  PFDFPRFLDLMAKHMKPEPFDRQLRDAFKVLDKDSTGFVAVSELRHILTNIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VGSDG+IR
Sbjct: 121 DEWIREVDVGSDGKIR 136

[Close]
BLAST of Lsi07G013420 vs. NCBI nr
Match: gi|848905602|ref|XP_012852243.1| (PREDICTED: probable calcium-binding protein CML13 [Erythranthe guttata])

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-63
Identity = 119/136 (87.50%), Postives = 132/136 (97.06%), Query Frame = 1 

Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSM+EAFTLFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+II+EE L A
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIISEEKLNA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RF+++MSKH+KPEPFDRQLRDAFKVLDKD TGYV+VS+LRHILTSIGEKLEP+EF
Sbjct: 61  PFDFARFIDLMSKHLKPEPFDRQLRDAFKVLDKDGTGYVVVSDLRHILTSIGEKLEPAEF 120

Query: 121 DEWIREVEVGSDGRIR 137
           DEWIREV+VGSDG+IR
Sbjct: 121 DEWIREVDVGSDGKIR 136

The following BLAST results are available for this feature:
BLAST of Lsi07G013420 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML13_ARATH6.5e-6182.48Probable calcium-binding protein CML13 OS=Arabidopsis thaliana GN=CML13 PE=2 SV=... [more]
CML14_ARATH2.3e-5878.83Probable calcium-binding protein CML14 OS=Arabidopsis thaliana GN=CML14 PE=2 SV=... [more]
CML7_ORYSJ5.3e-5575.56Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica GN=CML7 PE... [more]
CALM6_ARATH7.0e-3148.18Calmodulin-6 OS=Arabidopsis thaliana GN=CAM6 PE=1 SV=2[more]
CALM_EUPCH9.2e-3148.18Calmodulin OS=Euphorbia characias PE=2 SV=4[more]
BLAST of Lsi07G013420 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L2F2_CUCSA1.3e-6897.06Uncharacterized protein OS=Cucumis sativus GN=Csa_3G036390 PE=4 SV=1[more]
I1LGL7_SOYBN5.7e-6488.97Uncharacterized protein OS=Glycine max GN=GLYMA_11G030100 PE=4 SV=1[more]
A0A022QCM8_ERYGU1.7e-6387.50Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a015755mg PE=4 SV=1[more]
A0A067FK09_CITSI2.2e-6388.24Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g032081mg PE=4 SV=1[more]
A0A0D2Q0R6_GOSRA2.2e-6388.97Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_008G239400 PE=... [more]
BLAST of Lsi07G013420 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G12310.13.7e-6282.48 Calcium-binding EF-hand family protein[more]
AT1G62820.11.3e-5978.83 Calcium-binding EF-hand family protein[more]
AT5G21274.14.0e-3248.18 calmodulin 6[more]
AT3G43810.15.2e-3248.18 calmodulin 7[more]
AT3G56800.11.2e-3147.45 calmodulin 3[more]
BLAST of Lsi07G013420 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449460417|ref|XP_004147942.1|1.9e-6897.06PREDICTED: probable calcium-binding protein CML13 [Cucumis sativus][more]
gi|659096063|ref|XP_008448907.1|5.5e-6896.32PREDICTED: probable calcium-binding protein CML13 [Cucumis melo][more]
gi|502160156|ref|XP_004511652.1|3.7e-6488.97PREDICTED: probable calcium-binding protein CML13 [Cicer arietinum][more]
gi|947079238|gb|KRH28027.1|8.2e-6488.97hypothetical protein GLYMA_11G030100 [Glycine max][more]
gi|848905602|ref|XP_012852243.1|2.4e-6387.50PREDICTED: probable calcium-binding protein CML13 [Erythranthe guttata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G013420.1Lsi07G013420.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 13..41
score: 2.
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 82..136
score: 5.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 83..111
score: 4.7E-4coord: 12..40
score: 1.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 8..43
score: 12.979coord: 79..114
score: 12
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 8..69
score: 4.9E-20coord: 70..136
score: 6.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 5..136
score: 1.0
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 21..33
scor
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 1..136
score: 4.2E
NoneNo IPR availablePANTHERPTHR23050:SF214CALCIUM-BINDING PROTEIN CML14-RELATEDcoord: 1..136
score: 4.2E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi07G013420Csa3G036390Cucumber (Chinese Long) v2culsiB228
Lsi07G013420CSPI03G03130Wild cucumber (PI 183967)cpilsiB227
Lsi07G013420Cucsa.320480Cucumber (Gy14) v1cgylsiB530
Lsi07G013420CmaCh16G010440Cucurbita maxima (Rimu)cmalsiB327
Lsi07G013420CmaCh06G007560Cucurbita maxima (Rimu)cmalsiB775
Lsi07G013420CmoCh06G007780Cucurbita moschata (Rifu)cmolsiB766
Lsi07G013420CmoCh16G010920Cucurbita moschata (Rifu)cmolsiB316
Lsi07G013420Cla005687Watermelon (97103) v1lsiwmB428
Lsi07G013420MELO3C013982Melon (DHL92) v3.5.1lsimeB446
Lsi07G013420ClCG10G003120Watermelon (Charleston Gray)lsiwcgB389
Lsi07G013420MELO3C013982.2Melon (DHL92) v3.6.1lsimedB462
Lsi07G013420Cla97C10G187660Watermelon (97103) v2lsiwmbB395
Lsi07G013420BhiUN285G27Wax gourdlsiwgoB543
Lsi07G013420Cp4.1LG08g07480Cucurbita pepo (Zucchini)cpelsiB721
Lsi07G013420Cp4.1LG14g08610Cucurbita pepo (Zucchini)cpelsiB186
Lsi07G013420CsGy3G003060Cucumber (Gy14) v2cgyblsiB217
Lsi07G013420Carg25274Silver-seed gourdcarlsiB784
Lsi07G013420Carg21607Silver-seed gourdcarlsiB786
Lsi07G013420CsaV3_3G003160Cucumber (Chinese Long) v3cuclsiB250
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi07G013420Cucurbita moschata (Rifu)cmolsiB279
Lsi07G013420Cucurbita moschata (Rifu)cmolsiB572
Lsi07G013420Cucurbita pepo (Zucchini)cpelsiB156
Lsi07G013420Cucurbita pepo (Zucchini)cpelsiB631
Lsi07G013420Wild cucumber (PI 183967)cpilsiB390
Lsi07G013420Cucumber (Chinese Long) v2culsiB386
Lsi07G013420Melon (DHL92) v3.5.1lsimeB426
Lsi07G013420Watermelon (Charleston Gray)lsiwcgB394
Lsi07G013420Watermelon (97103) v1lsiwmB454
Lsi07G013420Cucumber (Gy14) v2cgyblsiB352
Lsi07G013420Melon (DHL92) v3.6.1lsimedB446
Lsi07G013420Cucumber (Chinese Long) v3cuclsiB409
Lsi07G013420Watermelon (97103) v2lsiwmbB391
Lsi07G013420Wax gourdlsiwgoB541
Lsi07G013420Bottle gourd (USVL1VR-Ls)lsilsiB019
Lsi07G013420Cucumber (Gy14) v1cgylsiB491
Lsi07G013420Cucurbita maxima (Rimu)cmalsiB291
Lsi07G013420Cucurbita maxima (Rimu)cmalsiB589