Lsi07G008750 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi07G008750
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionUbiquitin-conjugating enzyme E2 36
Locationchr07 : 9944412 .. 9948418 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAGATACAAATAATTGGGTCGTGCATCAGAACCTTCTTACCTTCAAAGCTTCCTTTCAATTCTTTATAATCTAGAGAGAGGAAAGCGTCTCCTCTCCCATTTCTCTCTCTTCCTTTCCTCTAAACCCTATCGTAACCTCTCAATCTTCCTTCCAATGGCGAACAGTAATCTTCCCCGAAGAATCATCAAGGTAACATCCCCGATCCTCTTCACGATCTCATTTCTTTTTTTCCCGATCCCTCTGTCTCTTTGATTTATTCTTTTGGTCAAGAAATCGTCTCCTTTCGCTGTTTGGATTTCGGTTATTCTTTTAGATTTATGTGTTAGTAGAATTGGGTTCTTGTTGCTACTGGATTTGATTTCCCCCCATCCTTTTTGTGATCAATCGCAGGAAACTCAGCGTCTCCTCAGTGAACCAGGTGAGTTTTGGATTCTTTTCGAGGGTGGGGGTTATTTGATTCCTGATCTTGTTACTGGGAGTTATTTGTTATTTGGGCCTTGGTTGTTGTTTGATGGCTTTTACGGTTTGGATTAATTCGACGGATAATTATGAAGCCGAAGTTTATTTTGATTTATTTGATCTCGATCTTTGATATGTTGTGTGATTTTAGCCCCTGGGATTAGTGCCTCGCCGTCAGAAGATAACATGCGATATTTTAACGTCATGATTCTTGGCCCGACTCAGTCACCCTATGAAGGTAAATTACTTGACAGTATGTTGTGGTTGTGGGAGCGTCAAATTATTTTGACATATGAATGGCTATAGAACCAACTATGTATTTAAGTGTAGATTCGCCTAAGTTTTATCGGTATTGGTCTTTGAAAATGACATTCACAGTGAAGATCAACCATGGGATTATCTTTTACTCAATGAAAAAGATATCTTATCTTCCTTTCTAACTTTGGTTTTGTGTCTACTGAAGTAATGAAAATGGGGAAATTTTAATTGTAATTTCAGGAAATACCTCTCTTTTTCCTTTATGCGCTATGGTGCAACAGTAGAATATTACTCTTCTTGGCTGCTAGACTCAGAAACATACTTGCTTACATTCTACTTAACATGGAATAAAAGTAATTTAGAGACGTAATCTGATTATCATTTCACTGATTTTATTGAATAATGTTATATGTATTCCTTTGTGTTCCTTTTCTCCTGACCCTTTGTCAAATCTATTGTCGTTAGTCTATTTCTATATGTTGTTATGTGCATATTTGTCCTGACTTCAAAGTACTTTGCAGGAGGAGTTTTCAAGTTGGAACTATTTTTACCTGAAGAATATCCTATGGCTGCTCCCAAGGTGATTTGTAATTCTTTCCTTCTGAGCTTATTAGATAAATTTTACTTTCATTTCTTCCACTAATATTCTTAAAAGGAAGTAGTATTTTTTTTTCATTAAAAGCAAAAAAGTATTAACAGAAGTATCCAGTAAAAATCATAGGGCTAATAGGGGAGCTCCAAATACTGTTCAAGATCATTTAGAACTTCTTACCCCAAATGTGCTGTTAAAATTCTCTTATACTTATTTAGCTACTTATATTTGCTTGTATTTACTTCTTGAATTATGATTTCACTTATGCATCGCCAGGGTGGTAAAACTAAAAGCTTCATCAGATACTAGATCTGCCATCATTATAATATTCTCTGTCCCTTTCGTTTCATCCCCCCTCTCAAGGGTTTCCTATCTTGCACTGATGCAACATAAAAAGATCAGAAGCCATCATAGTTTACTTTACATACACGTAGGTTTTTCTATTTCACGAATCATGATATTGACAATATAACTCAATTCTGGTTGATCTATGAAACACATGGGAGCAAATTGATACTACCTGAAGGGAGTTTTTGGTAGGGAGATCCTTACGGTTGGAGATGTAGTAGCTTATTTATGACTTGCTTTCTCAGGCATAGGTTCATAGTTTTGAAAGAATGAGAGGAAACATGTTTGTCTGGTTCTTGCTAGAGATCCAGTTGTGTGGGATGAAATTCATGGCAAACTGTTTTTGGCAATTGTTGCAAACTTCTTCCTTTTCTCATTTTGCATAGAAAACCAACTTTCATGAAGAAGATGAAAGAACAAAATACAAGGAGAGGCCCACAAAAATAAGGGCCTAAACGGAACAGGGGAAGCATCCCTCAGAACATTTACAAAATCGACCCCCCAAAGCATCCCCCAAACTTGTAATCCAATTAGGCTAGCGTTTTCTTTTTCCATTCCTGTAGAATAAATCAAACTAACTACTTTTATTTTTATTTTTGAAGTTTCTATCATTAATTCTTGTAGAATGTACGTAGTGATGTAAGTATGCCTGAAAGTACTTTATTTTCTTAGCTTATGTATGGAAAGTATTTGTCTGCCTGACTGGACTGTCATGGAAGGGAATAAGCTGTTCATAGATTTAATAATTTGCTCCATACTGACGATAATTCAGTTATCATTCGTTATTCTTTATTTTTTGTAGTACATGTAGTCATGTATTCAGTAGAGTGGGGTTGTATATCTGGTTTTGTTTTATCTTATTTTGTGTCAAACCTGTTGATAGAGGGGGGGTTATGCCTGAAAGGCTGAACAATTGACACTCATTACTCATAAGCTAACTAATTTAACTTGAGTGGTTAATCTTGGTCTGTGTCTTTTGCGTACTATTCTTATAAATGGAAAATTGATCCACTCAGAAAAGAGATGTAAAACCAGGGAAATGTTTAAGCAACGTCAAACAATCTTTTTTCGAAGAGTTTAATTTATTGCTCCTTGATGTCCCATAACTGCAGATTCTGTATTTAGGTACACTAAGTGGTCCTTTTGGAAGTTAATGAAACCTTTGATTGCCTCCACCACCATCTTTTCCCCTTCCTCTGTTTTCTTTTCCATTTTCACTTATACATTTTCCTTTTTTTGTGTGATTATTTTTTGTGGTGCAGGTTCGATTTCTCACAAAGATTTATCATCCGAATATTGACAAGGTATGTGTCTACTTGTGTAGTTCTTATTGCCACCCTCACCAACATGCTGCAGTTTGGTTATTGATGGTTTGACTTTCTGTAATTAAGCTTGGGAGGATATGCCTTGATATTCTGAAAGACAAATGGAGTCCAGCTCTACAGATACGAACTGTACTTTTGAGGTATTACAATTACAATGTTAGATGTGATTTTTCAGTGACTGCATATCAGCTTTGAACATTTTGGTCATTTCCTTGGGAATATTTATCTTTCTACAATAATTCCTATAATTATACAAAAATTGAAGAAATTCTGTTACTAGTGGCATCCATTTCTTCTCTTCTTGTTCTACGTCCCCTTTCTATGAAATTTTTTGACTAACAGTTACCTTTTCCATCACTTTCTGTCAGTATTCAAGCACTTCTAAGTGCTCCAAACCCAGATGATCCACTATCCGAGAACATTGCCAAACATTGGAAAACAAATGAGGCAGAAGCTGTTGAAACAGGTGAGTTGGTTTAAATCATTGTTTTCTTAAGCTTTTTTCTTAATTAAACTTTTGTAGTCTTAGTTTAAGCCTTGTGTCAATTTGGGTCCTCTGTTCTTAATAGTTCTAATGTCATCATTTAGGTATTAATTAAGGCTTGTTCTTGACGTCTCTTTAGTTGAGGGGTTGTGAATGAGGTAAGATGAGATGGCATGAGGTGGTATTCGATTTCATCGACTACGGGGGACTGAGTATTATTTGGGTTGAAAGCTAATGAATTGAATTGAGTTTTAAGGGCAGGGTTTAGATCTTTTATGTCATATAAAAACAGAAAAGGACATTAGGGGAAAAGGGAGGGAGGGGCACCGTCGGGGGAAGGGATATTTGTCGGAGAAAATTGCACAACTGCCCCATTTTGGAGGCTCAAAATGAAAAATGCTCCACTTTTTAAAAACAATGGTAATTGACATTCGTCATATCTATGTGAAGTTATGAATTTGCCACTGAACACCAGAGAGTGAGAGTGCAAGAGCGAGAGTAAGAGAGCGAGACCGTGAGAGAAAGAAAGCGTGTGCAA

mRNA sequence

GAAAGATACAAATAATTGGGTCGTGCATCAGAACCTTCTTACCTTCAAAGCTTCCTTTCAATTCTTTATAATCTAGAGAGAGGAAAGCGTCTCCTCTCCCATTTCTCTCTCTTCCTTTCCTCTAAACCCTATCGTAACCTCTCAATCTTCCTTCCAATGGCGAACAGTAATCTTCCCCGAAGAATCATCAAGGAAACTCAGCGTCTCCTCAGTGAACCAGGTGAGTTTTGGATTCTTTTCGAGGGTGGGGGTTATTTGATTCCTGATCTTGTTACTGGGAGTTATTTGTTATTTGGGCCTTGGTTGTTGTTTGATGGCTTTTACGCCCCTGGGATTAGTGCCTCGCCGTCAGAAGATAACATGCGATATTTTAACGTCATGATTCTTGGCCCGACTCAGTCACCCTATGAAGGAGGAGTTTTCAAGTTGGAACTATTTTTACCTGAAGAATATCCTATGGCTGCTCCCAAGGTTCGATTTCTCACAAAGATTTATCATCCGAATATTGACAAGCTTGGGAGGATATGCCTTGATATTCTGAAAGACAAATGGAGTCCAGCTCTACAGATACGAACTGTACTTTTGAGTATTCAAGCACTTCTAAGTGCTCCAAACCCAGATGATCCACTATCCGAGAACATTGCCAAACATTGGAAAACAAATGAGGCAGAAGCTGTTGAAACAGGTGAGTTGGTTTAAATCATTGTTTTCTTAAGCTTTTTTCTTAATTAAACTTTTGTAGTCTTAGTTTAAGCCTTGTGTCAATTTGGGTCCTCTGTTCTTAATAGTTCTAATGTCATCATTTAGGTATTAATTAAGGCTTGTTCTTGACGTCTCTTTAGTTGAGGGGTTGTGAATGAGGTAAGATGAGATGGCATGAGGTGGTATTCGATTTCATCGACTACGGGGGACTGAGTATTATTTGGGTTGAAAGCTAATGAATTGAATTGAGTTTTAAGGGCAGGGTTTAGATCTTTTATGTCATATAAAAACAGAAAAGGACATTAGGGGAAAAGGGAGGGAGGGGCACCGTCGGGGGAAGGGATATTTGTCGGAGAAAATTGCACAACTGCCCCATTTTGGAGGCTCAAAATGAAAAATGCTCCACTTTTTAAAAACAATGGTAATTGACATTCGTCATATCTATGTGAAGTTATGAATTTGCCACTGAACACCAGAGAGTGAGAGTGCAAGAGCGAGAGTAAGAGAGCGAGACCGTGAGAGAAAGAAAGCGTGTGCAA

Coding sequence (CDS)

ATGGCGAACAGTAATCTTCCCCGAAGAATCATCAAGGAAACTCAGCGTCTCCTCAGTGAACCAGGTGAGTTTTGGATTCTTTTCGAGGGTGGGGGTTATTTGATTCCTGATCTTGTTACTGGGAGTTATTTGTTATTTGGGCCTTGGTTGTTGTTTGATGGCTTTTACGCCCCTGGGATTAGTGCCTCGCCGTCAGAAGATAACATGCGATATTTTAACGTCATGATTCTTGGCCCGACTCAGTCACCCTATGAAGGAGGAGTTTTCAAGTTGGAACTATTTTTACCTGAAGAATATCCTATGGCTGCTCCCAAGGTTCGATTTCTCACAAAGATTTATCATCCGAATATTGACAAGCTTGGGAGGATATGCCTTGATATTCTGAAAGACAAATGGAGTCCAGCTCTACAGATACGAACTGTACTTTTGAGTATTCAAGCACTTCTAAGTGCTCCAAACCCAGATGATCCACTATCCGAGAACATTGCCAAACATTGGAAAACAAATGAGGCAGAAGCTGTTGAAACAGGTGAGTTGGTTTAA

Protein sequence

MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGELV
BLAST of Lsi07G008750 vs. Swiss-Prot
Match: UBC35_ARATH (Ubiquitin-conjugating enzyme E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 5.2e-66
Identity = 134/178 (75.28%), Postives = 137/178 (76.97%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWK+NEAEAV+T +
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAK 143

BLAST of Lsi07G008750 vs. Swiss-Prot
Match: UBC36_ARATH (Ubiquitin-conjugating enzyme E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 6.8e-66
Identity = 134/178 (75.28%), Postives = 137/178 (76.97%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSE+NMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWK+NEAEAVET +
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAK 143

BLAST of Lsi07G008750 vs. Swiss-Prot
Match: UBC13_CAEEL (Ubiquitin-conjugating enzyme E2 13 OS=Caenorhabditis elegans GN=ubc-13 PE=1 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 1.6e-54
Identity = 99/121 (81.82%), Postives = 111/121 (91.74%), Query Frame = 1

Query: 58  PGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNI 117
           PGISA+P E N RYF+VMI GP  SP+ GGVFKLELFLPEEYPMAAPKVRF+TKIYHPNI
Sbjct: 22  PGISANPDESNARYFHVMIAGPDDSPFAGGVFKLELFLPEEYPMAAPKVRFMTKIYHPNI 81

Query: 118 DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG 177
           DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP+DPL+ ++A+ WKTNEAEA++T 
Sbjct: 82  DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPEDPLATDVAEQWKTNEAEAIKTA 141

Query: 178 E 179
           +
Sbjct: 142 K 142

BLAST of Lsi07G008750 vs. Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.5e-54
Identity = 97/119 (81.51%), Postives = 108/119 (90.76%), Query Frame = 1

Query: 58  PGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNI 117
           PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPEEYPMAAPKVRF+TKIYHPN+
Sbjct: 21  PGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNV 80

Query: 118 DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVET 177
           DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL+ ++A+ WKTNEA+A+ET
Sbjct: 81  DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139

BLAST of Lsi07G008750 vs. Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.5e-54
Identity = 97/119 (81.51%), Postives = 108/119 (90.76%), Query Frame = 1

Query: 58  PGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNI 117
           PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPEEYPMAAPKVRF+TKIYHPN+
Sbjct: 21  PGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNV 80

Query: 118 DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVET 177
           DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL+ ++A+ WKTNEA+A+ET
Sbjct: 81  DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIET 139

BLAST of Lsi07G008750 vs. TrEMBL
Match: F4I615_ARATH (Ubiquitin-conjugating enzyme E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=3 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.2e-71
Identity = 141/178 (79.21%), Postives = 146/178 (82.02%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKETQRLLSEPG F   F+                           +PGI
Sbjct: 1   MANSNLPRRIIKETQRLLSEPGNFITSFDP--------------------------SPGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSE+NMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWK+NEAEAVET +
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAK 152

BLAST of Lsi07G008750 vs. TrEMBL
Match: A0A0D2SFU0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G173200 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 8.1e-66
Identity = 137/179 (76.54%), Postives = 139/179 (77.65%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGEL 180
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG++
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGKI 144

BLAST of Lsi07G008750 vs. TrEMBL
Match: A0A061F987_THECC (Ubiquitin-conjugating enzyme 36 isoform 2 OS=Theobroma cacao GN=TCM_031769 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-65
Identity = 137/177 (77.40%), Postives = 137/177 (77.40%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 52  MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 111

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 112 SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 171

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG 178
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG
Sbjct: 172 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG 193

BLAST of Lsi07G008750 vs. TrEMBL
Match: A0A0D2Q0K6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G042900 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 4.0e-65
Identity = 136/178 (76.40%), Postives = 138/178 (77.53%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           +ASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  NASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG+
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGK 143

BLAST of Lsi07G008750 vs. TrEMBL
Match: E2CW93_CUCSA (Putative ubiquitin-conjugating enzyme OS=Cucumis sativus GN=UBC13 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 9.0e-65
Identity = 136/178 (76.40%), Postives = 137/178 (76.97%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVET +
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAK 143

BLAST of Lsi07G008750 vs. TAIR10
Match: AT1G16890.3 (AT1G16890.3 ubiquitin-conjugating enzyme 36)

HSP 1 Score: 276.6 bits (706), Expect = 1.1e-74
Identity = 141/178 (79.21%), Postives = 146/178 (82.02%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKETQRLLSEPG F   F+                           +PGI
Sbjct: 1   MANSNLPRRIIKETQRLLSEPGNFITSFDP--------------------------SPGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSE+NMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWK+NEAEAVET +
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAK 152

BLAST of Lsi07G008750 vs. TAIR10
Match: AT1G78870.1 (AT1G78870.1 ubiquitin-conjugating enzyme 35)

HSP 1 Score: 247.3 bits (630), Expect = 7.3e-66
Identity = 134/179 (74.86%), Postives = 137/179 (76.54%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEG-GVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDK 120
           SASPSEDNMRYFNVMILGPTQSPYEG GVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDK
Sbjct: 61  SASPSEDNMRYFNVMILGPTQSPYEGRGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDK 120

Query: 121 LGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           LGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWK+NEAEAV+T +
Sbjct: 121 LGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAK 144

BLAST of Lsi07G008750 vs. TAIR10
Match: AT2G16740.1 (AT2G16740.1 ubiquitin-conjugating enzyme 29)

HSP 1 Score: 130.6 bits (327), Expect = 9.9e-31
Identity = 60/112 (53.57%), Postives = 80/112 (71.43%), Query Frame = 1

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SA P+ ++M ++   I+GP +SPY GGVF + +  P +YP   PKV F TK++HPNI+  
Sbjct: 22  SAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPFKPPKVVFRTKVFHPNINSN 81

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAE 173
           G ICLDILKD+WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT++ +
Sbjct: 82  GNICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHIYKTDKTK 133

BLAST of Lsi07G008750 vs. TAIR10
Match: AT3G08690.1 (AT3G08690.1 ubiquitin-conjugating enzyme 11)

HSP 1 Score: 130.2 bits (326), Expect = 1.3e-30
Identity = 61/116 (52.59%), Postives = 80/116 (68.97%), Query Frame = 1

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SA P  ++M ++   I+GP +SPY GGVF + +  P +YP   PKV F TK+YHPNI+  
Sbjct: 22  SAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSN 81

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVET 177
           G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT+ ++   T
Sbjct: 82  GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYEST 137

BLAST of Lsi07G008750 vs. TAIR10
Match: AT5G41700.4 (AT5G41700.4 ubiquitin conjugating enzyme 8)

HSP 1 Score: 129.0 bits (323), Expect = 2.9e-30
Identity = 60/113 (53.10%), Postives = 79/113 (69.91%), Query Frame = 1

Query: 60  ISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDK 119
           I A P  ++M ++   I+GP +SPY GGVF + +  P +YP   PKV F TK++HPNI+ 
Sbjct: 22  IFAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINS 81

Query: 120 LGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAE 173
            G ICLDILK++WSPAL I  VLLSI +LL+ PNPDDPL   IA  +KT+ A+
Sbjct: 82  NGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAK 134

BLAST of Lsi07G008750 vs. NCBI nr
Match: gi|334182638|ref|NP_001185017.1| (ubiquitin-conjugating enzyme E2 36 [Arabidopsis thaliana])

HSP 1 Score: 276.6 bits (706), Expect = 3.2e-71
Identity = 141/178 (79.21%), Postives = 146/178 (82.02%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKETQRLLSEPG F   F+                           +PGI
Sbjct: 1   MANSNLPRRIIKETQRLLSEPGNFITSFDP--------------------------SPGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSE+NMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWK+NEAEAVET +
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAK 152

BLAST of Lsi07G008750 vs. NCBI nr
Match: gi|823217102|ref|XP_012441222.1| (PREDICTED: ubiquitin-conjugating enzyme E2 36-like isoform X1 [Gossypium raimondii])

HSP 1 Score: 260.0 bits (663), Expect = 3.1e-66
Identity = 141/180 (78.33%), Postives = 147/180 (81.67%), Query Frame = 1

Query: 1   MANSNLPRRIIKE--TQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAP 60
           MAN+NLPRRIIK   +  LLS  G   I F     L+  L+    L     LL +   AP
Sbjct: 1   MANTNLPRRIIKVPFSISLLSFSGFLHIAFIA---LLFTLLMNQKLQETQRLLSEP--AP 60

Query: 61  GISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID 120
           GISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPM APKVRFLTKIYHPNID
Sbjct: 61  GISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMTAPKVRFLTKIYHPNID 120

Query: 121 KLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           KLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVET +
Sbjct: 121 KLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAK 175

BLAST of Lsi07G008750 vs. NCBI nr
Match: gi|763815190|gb|KJB82042.1| (hypothetical protein B456_013G173200 [Gossypium raimondii])

HSP 1 Score: 258.1 bits (658), Expect = 1.2e-65
Identity = 137/179 (76.54%), Postives = 139/179 (77.65%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGEL 180
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG++
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGKI 144

BLAST of Lsi07G008750 vs. NCBI nr
Match: gi|590610106|ref|XP_007021722.1| (Ubiquitin-conjugating enzyme 36 isoform 2 [Theobroma cacao])

HSP 1 Score: 256.9 bits (655), Expect = 2.6e-65
Identity = 137/177 (77.40%), Postives = 137/177 (77.40%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 52  MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 111

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 112 SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 171

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG 178
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG
Sbjct: 172 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG 193

BLAST of Lsi07G008750 vs. NCBI nr
Match: gi|763745450|gb|KJB12889.1| (hypothetical protein B456_002G042900 [Gossypium raimondii])

HSP 1 Score: 255.8 bits (652), Expect = 5.8e-65
Identity = 136/178 (76.40%), Postives = 138/178 (77.53%), Query Frame = 1

Query: 1   MANSNLPRRIIKETQRLLSEPGEFWILFEGGGYLIPDLVTGSYLLFGPWLLFDGFYAPGI 60
           MANSNLPRRIIKE                              LL  P        APGI
Sbjct: 1   MANSNLPRRIIKE---------------------------TQRLLSEP--------APGI 60

Query: 61  SASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120
           +ASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL
Sbjct: 61  NASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 120

Query: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGE 179
           GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETG+
Sbjct: 121 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETGK 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
UBC35_ARATH5.2e-6675.28Ubiquitin-conjugating enzyme E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1[more]
UBC36_ARATH6.8e-6675.28Ubiquitin-conjugating enzyme E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1[more]
UBC13_CAEEL1.6e-5481.82Ubiquitin-conjugating enzyme E2 13 OS=Caenorhabditis elegans GN=ubc-13 PE=1 SV=2[more]
UBE2N_PONAB3.5e-5481.51Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1[more]
UBE2N_MOUSE3.5e-5481.51Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1[more]
Match NameE-valueIdentityDescription
F4I615_ARATH2.2e-7179.21Ubiquitin-conjugating enzyme E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=3 SV=1[more]
A0A0D2SFU0_GOSRA8.1e-6676.54Uncharacterized protein OS=Gossypium raimondii GN=B456_013G173200 PE=3 SV=1[more]
A0A061F987_THECC1.8e-6577.40Ubiquitin-conjugating enzyme 36 isoform 2 OS=Theobroma cacao GN=TCM_031769 PE=3 ... [more]
A0A0D2Q0K6_GOSRA4.0e-6576.40Uncharacterized protein OS=Gossypium raimondii GN=B456_002G042900 PE=3 SV=1[more]
E2CW93_CUCSA9.0e-6576.40Putative ubiquitin-conjugating enzyme OS=Cucumis sativus GN=UBC13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G16890.31.1e-7479.21 ubiquitin-conjugating enzyme 36[more]
AT1G78870.17.3e-6674.86 ubiquitin-conjugating enzyme 35[more]
AT2G16740.19.9e-3153.57 ubiquitin-conjugating enzyme 29[more]
AT3G08690.11.3e-3052.59 ubiquitin-conjugating enzyme 11[more]
AT5G41700.42.9e-3053.10 ubiquitin conjugating enzyme 8[more]
Match NameE-valueIdentityDescription
gi|334182638|ref|NP_001185017.1|3.2e-7179.21ubiquitin-conjugating enzyme E2 36 [Arabidopsis thaliana][more]
gi|823217102|ref|XP_012441222.1|3.1e-6678.33PREDICTED: ubiquitin-conjugating enzyme E2 36-like isoform X1 [Gossypium raimond... [more]
gi|763815190|gb|KJB82042.1|1.2e-6576.54hypothetical protein B456_013G173200 [Gossypium raimondii][more]
gi|590610106|ref|XP_007021722.1|2.6e-6577.40Ubiquitin-conjugating enzyme 36 isoform 2 [Theobroma cacao][more]
gi|763745450|gb|KJB12889.1|5.8e-6576.40hypothetical protein B456_002G042900 [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR023313UBQ-conjugating_AS
IPR016135UBQ-conjugating_enzyme/RWD
IPR000608UBQ-conjugat_E2
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006301 postreplication repair
biological_process GO:0070534 protein K63-linked ubiquitination
biological_process GO:0010039 response to iron ion
biological_process GO:0010053 root epidermal cell differentiation
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0008150 biological_process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0031372 UBC13-MMS2 complex
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005634 nucleus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0031625 ubiquitin protein ligase binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G008750.1Lsi07G008750.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 58..172
score: 2.0
IPR000608Ubiquitin-conjugating enzyme E2PROFILEPS50127UBIQUITIN_CONJUGAT_2coord: 58..175
score: 3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 2..175
score: 3.8
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 58..172
score: 1.05
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBIQUITIN_CONJUGAT_1coord: 113..128
scor
NoneNo IPR availablePANTHERPTHR24067UBIQUITIN-CONJUGATING ENZYME E2coord: 58..176
score: 2.0E-101coord: 1..22
score: 2.0E
NoneNo IPR availablePANTHERPTHR24067:SF159UBIQUITIN-CONJUGATING ENZYME E2 36coord: 58..176
score: 2.0E-101coord: 1..22
score: 2.0E
NoneNo IPR availableSMARTSM00212ubc_7coord: 48..178
score: 9.4