Lsi07G002290 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi07G002290
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Pectinesterase
Location	chr07 : 2501961 .. 2506091 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATACATTCCAAGGAAAAGACAATGAAAAATAGCATTTAAACTGAGAAGTCACAGATGGGTCTGAAATTTTAATGACTAAACAGACAATTTGGATTCAGACACACACAACTAGCTACCTGCAAGATTCCCCCAATCTTGGTCTCTCTCTTCCCCCATTTATCTCTTTTTCATCTTTCTTTTAAAGCCTCTCATTCCTTCAAGACTGAAGAACAACACGATGGTTGTTCCTGTTATGAAGCTAATTCTGTTTCTGCTCATCACCATAATGCTGCCCGGCACCGCCCTTTCTTACGGCCAAAACTGGACGGTGGGCAGCGGCGGCCGGGGAATGAGAACAGTGGAAGATGAATACTTGAAATGGATAAGACATATGGGCTCTTTCAAGCACTCCCTTTTTCAAAAGACGAAGAATAAGTTCAAGCCTTGTTTGACACTGAAGGTTTCTAAGAACACCAAGGCGGGGGGTTTTCGCTCGCTTCAGAAGGCTATAAGCTCTCTGCCGGTGATCAACCGGTGCCGGGTCCTCATCCGTGTCGCTGCTGGAATCTATAGGTCAGTGTCTACTTTCTTTCTTGTAGTGTTGAAAGAAAACTATATACTTGCAAGCATGCTGAACTATACTTATCTTTTAAATTACTGTGAGACCTAGAGAGAAAGTTGAGATTCCCGCGATGATGAGTTACATATGGGTGGAGGGTGAGGGAGTGGAGAAGACGATAATTGAATGGGGCGACACCGCCGACCATATGGGAGACAATGGCCGCCCCATGGGCACATTTGCTTCTGCAACTTTTGCTGTTAACTCTCCTTTCTTCATCGCTACCAACATCACCTTCAAGGTATGAACTTTTTATCTTTCTTTCTTTCCTTTTACTAAATAAAAAAAAGGCCTTTGAAATTTGTCTTTTATTAAAAAGATCTCAAAAGTTTCAAAATTATCTTTGACATTTTATAAGCCGTTCAAACCTACATTTGAAAATTTGAAAATAGTTAGAATGTTGAATTAAAATTAGGATCTAAAAGGGTGTGATACAATAATAGTGACAAACAAAAATTGAAACTGAAATATCGTCAGATTAGTCTCAATTTTCATTTAAATTCAAATAAATTTTTATTTTAATAGTAGTTTTATTTAGATATTTATAAAATGTCAAAGGTAATTTAGCAACTTTTAAAAAAATATAAATATTATTTTTTTAAAGTAATCTTTTTTTAATTTTTTTTTTTTGACAATATGTGAGATGGAGAATCAACTTCTGACTTTAAGGTTGAAAGTACAAGTACTATGTTTTGACAAAGTTACATTACATCTAGATACTTTTTAGATTTAAATTATATTTTTCTTAAGATTATATATATATATAGATATATATATAGATACATTTATTTACAACTACATCTCAATTTTAATGTGCAAAGTAAAAGAATGAAACTAAAATATTTGAAATTTTAGTAAACTTCACCTTGTTATATTTTATTACAATGTTTTGACTTATATCCAAAATTTAAAATATCGATGTCGATGGAAATATTGAAGTTTTAATTTTATTAACATTTCAATGAAAATATCGATTTCGATAAATATTTCTAGAAAAATTATAAAAATAAATTTAAAACAGCAAATAAACTTTTTATTATTTTCAAATAAGTAAATATGTCTATTATTTATATTATATTATATTAATGGCATTTTGACGCTTATTTTTCGTGTTTCGTAGATTTTTCTACAATATAATAAAAATATTGATTCACCTCTCGTATCGATATCGAACCCATAAAAATATGAAAATATTGATGAAAATTTAATAATATGCTTATATCATCTTTCGTAAGTGAAAAAACTTGGATAATTACATTATCATTATTGTACTAGTAGTACCATTGCTATTTTGAATTTAAAGGACATGGGAGATTATGATATTTTGACTTTTCAGATTTTTTTTTTTTTTTAATATTTAACATGTGAACTTGAAGAATTGTGTGTGTGTGTGTTTATAAACCATGGTCTCCGAATTTTCGAAACTAAAGAAATTTCTCTAGTTTAAATTTTTTATCTCAATAATAATTTAATAATGTTTGTGACTTTTTTTTAGAAAATTTCCTATATGTTAATCTATTGAAATATTTTATAATATAATTACTTAAATTATGTTTACAAACATATGACGGTGTACATATTTTAATAGAGCTAAAAATACAAATGAAGAATAGAAGGAATAAAGTTGAAGTGTCGTATGTAGATGGCAATTTGCTCGTGAGTTTCAATTGACCAAACTGACATGTATATATATTTATATATATATATTATATATATATAGGAAGGGACATGTAGTTCTTGGAAGGACTTGGAAGAGAATTAAAAATATCATTATAATCAAGATGAAATTAAAAATGTTCATTTAATTTAAGAGAGAATAAATTAATATTTATTCAAATATAGTTCTCAATCTTTATATTTTAATTTCCGTACGTACTTAAAAAATTGATTCGTCCTCTTCTCTTATCCACTTATATTTTAGTCCTCCAACTTTGTAAAATTTCAAACTTAACTCTCTTTTTTTTTTCTGAGAATAATTCAAACTTAACTCTTCAACTTTTATTAAGATTTAGATTATAATCATACTTTGGTGCCTATCTTCGAAACTTCCAAAATATTGTGCTTCCTTGAACTTATTTTCCAGAAAAAATATACTTTTGATTCTTAAATTTGATTTACAATATTTGGAGTTCATTAGTTTTTAAAATTTAACAATGTTATCTCTAGTCATTTTGTCAGATATTTTTTTGTTAACGAAAAACTAACCTATCGTCTATTTTTTTTATTATTATATTTTTATTTTTGAAGTTTGGTTTATATCACATTTTCCCTTAGTCGATGTCTCTCTTATTTAAGATTGATGCACAGAGATTTGTTCTCCCATTTTATACCTTCGGTTTTTGAACGAGAGAGTTTGAGGTAAGTAGTAGGTAAATAGTGGTGTGCGATTGCGGTTGTTCGATAAATGAAGGAAAAAGAAGATATATATATATATATATATATATATACACACACATATTAAAACATTATTAACTTAAAAAAACCTCACATCATCTTGGTTAGTTAATTAATGAAAATTGACGATTACAGGACCAATAAAACTAATTTTCGATACCTAAGGGGTTAAATGTTGAAATTATTATGAAATCTAAATGCTACCCAAACCAAAAGTATATTTGTCACCTTCAAAAAATTAGGAGCAAGTATCATCATAAATGGTTAAAGAAAAATGTTTGCACTAAGTACCATTACATTGCTCGTCCCCACCCCCTCCCTCCAAAAAAAGAACAAGGCAAGGCTTCCTCCGTCAGGAGCATTGGGAAAGCAAGCAGTGGCATTCAGAATATCAGGGGATGCGGCAACATTCATAAGTTGCAGGTTCATTGGCGCCCAGGATACATTATACGACCACATGGGGCGCCACTACTTTAAAGACTGCTACATCGAAGGGTCGGTGGACTTCGTGTTCGGTGACGGTCGCTCTATCTATGATGGTTGCCATTTGCATGCCATCACCAATAGTTACGGCGCACTCACAGCCCAGAAGAGGAATAGCCTGCTTGAGGAAACTGGCTTCTCATTCCTTAATTGTAAGGTTTCTGGCTCAGGCGCTCTGTATTTGGGTAGAGCATGGGGTAGCTTCTCCAGAGTTGTATTTGCTTATACCTTTATGGATAAAATCATCACTCCCACTGGTTGGTATAACTGGGGCGACAAAAATCGTGAATTGTAAGTCTTTTTCTTGTTCTTTTTCTTGTAATACAATATAAAACAATATTGGAGTTATTTATTTATTTATTTGGATGTAGGACTGTGTTTTATGGACAATATCGATGTTCAGGACCAGGAGCTGATTATGGAGGACGAGTTTCGTGGTCTAGAGAACTCACCGAGTCAGAAGCCAACCCATTTCTCTCTCTTGATTTTATTAATGCAAACCAATGGCTTCCAAATTATCACACTCATCTCGCCTTGAAACCAAATTGATTGTACCCTTTCCTCATTTTAATTGTTTTACTTTCTTTTAATCATGTTATGACCTACCAATGTTTTTGATATAACAGGTATTAATAAATTTTATTTATTTTTTTTCA

mRNA sequence

ATACATTCCAAGGAAAAGACAATGAAAAATAGCATTTAAACTGAGAAGTCACAGATGGGTCTGAAATTTTAATGACTAAACAGACAATTTGGATTCAGACACACACAACTAGCTACCTGCAAGATTCCCCCAATCTTGGTCTCTCTCTTCCCCCATTTATCTCTTTTTCATCTTTCTTTTAAAGCCTCTCATTCCTTCAAGACTGAAGAACAACACGATGGTTGTTCCTGTTATGAAGCTAATTCTGTTTCTGCTCATCACCATAATGCTGCCCGGCACCGCCCTTTCTTACGGCCAAAACTGGACGGTGGGCAGCGGCGGCCGGGGAATGAGAACAGTGGAAGATGAATACTTGAAATGGATAAGACATATGGGCTCTTTCAAGCACTCCCTTTTTCAAAAGACGAAGAATAAGTTCAAGCCTTGTTTGACACTGAAGGTTTCTAAGAACACCAAGGCGGGGGGTTTTCGCTCGCTTCAGAAGGCTATAAGCTCTCTGCCGGTGATCAACCGGTGCCGGGTCCTCATCCGTGTCGCTGCTGGAATCTATAGTACCATTACATTGCTCGTCCCCACCCCCTCCCTCCAAAAAAAGAACAAGGCAAGGCTTCCTCCGTCAGGAGCATTGGGAAAGCAAGCAGTGGCATTCAGAATATCAGGGGATGCGGCAACATTCATAAGTTGCAGGTTCATTGGCGCCCAGGATACATTATACGACCACATGGGGCGCCACTACTTTAAAGACTGCTACATCGAAGGGTCGGTGGACTTCGTGTTCGGTGACGGTCGCTCTATCTATGATGGTTGCCATTTGCATGCCATCACCAATAGTTACGGCGCACTCACAGCCCAGAAGAGGAATAGCCTGCTTGAGGAAACTGGCTTCTCATTCCTTAATTGTAAGGTTTCTGGCTCAGGCGCTCTGTATTTGGGTAGAGCATGGGGTAGCTTCTCCAGAGTTGTATTTGCTTATACCTTTATGGATAAAATCATCACTCCCACTGGTTGGTATAACTGGGGCGACAAAAATCGTGAATTGACTGTGTTTTATGGACAATATCGATGTTCAGGACCAGGAGCTGATTATGGAGGACGAGTTTCGTGGTCTAGAGAACTCACCGAGTCAGAAGCCAACCCATTTCTCTCTCTTGATTTTATTAATGCAAACCAATGGCTTCCAAATTATCACACTCATCTCGCCTTGAAACCAAATTGATTGTACCCTTTCCTCATTTTAATTGTTTTACTTTCTTTTAATCATGTTATGACCTACCAATGTTTTTGATATAACAGGTATTAATAAATTTTATTTATTTTTTTTCA

Coding sequence (CDS)

ATGGTTGTTCCTGTTATGAAGCTAATTCTGTTTCTGCTCATCACCATAATGCTGCCCGGCACCGCCCTTTCTTACGGCCAAAACTGGACGGTGGGCAGCGGCGGCCGGGGAATGAGAACAGTGGAAGATGAATACTTGAAATGGATAAGACATATGGGCTCTTTCAAGCACTCCCTTTTTCAAAAGACGAAGAATAAGTTCAAGCCTTGTTTGACACTGAAGGTTTCTAAGAACACCAAGGCGGGGGGTTTTCGCTCGCTTCAGAAGGCTATAAGCTCTCTGCCGGTGATCAACCGGTGCCGGGTCCTCATCCGTGTCGCTGCTGGAATCTATAGTACCATTACATTGCTCGTCCCCACCCCCTCCCTCCAAAAAAAGAACAAGGCAAGGCTTCCTCCGTCAGGAGCATTGGGAAAGCAAGCAGTGGCATTCAGAATATCAGGGGATGCGGCAACATTCATAAGTTGCAGGTTCATTGGCGCCCAGGATACATTATACGACCACATGGGGCGCCACTACTTTAAAGACTGCTACATCGAAGGGTCGGTGGACTTCGTGTTCGGTGACGGTCGCTCTATCTATGATGGTTGCCATTTGCATGCCATCACCAATAGTTACGGCGCACTCACAGCCCAGAAGAGGAATAGCCTGCTTGAGGAAACTGGCTTCTCATTCCTTAATTGTAAGGTTTCTGGCTCAGGCGCTCTGTATTTGGGTAGAGCATGGGGTAGCTTCTCCAGAGTTGTATTTGCTTATACCTTTATGGATAAAATCATCACTCCCACTGGTTGGTATAACTGGGGCGACAAAAATCGTGAATTGACTGTGTTTTATGGACAATATCGATGTTCAGGACCAGGAGCTGATTATGGAGGACGAGTTTCGTGGTCTAGAGAACTCACCGAGTCAGAAGCCAACCCATTTCTCTCTCTTGATTTTATTAATGCAAACCAATGGCTTCCAAATTATCACACTCATCTCGCCTTGAAACCAAATTGA

Protein sequence

MVVPVMKLILFLLITIMLPGTALSYGQNWTVGSGGRGMRTVEDEYLKWIRHMGSFKHSLFQKTKNKFKPCLTLKVSKNTKAGGFRSLQKAISSLPVINRCRVLIRVAAGIYSTITLLVPTPSLQKKNKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDFINANQWLPNYHTHLALKPN

Homology

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BLAST of Lsi07G002290 vs. Swiss-Prot
Match: PME53_ARATH (Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.0e-83
Identity = 136/217 (62.67%), Postives = 175/217 (80.65%), Query Frame = 1


Query: 109 GIYSTITLLVPTP-----SLQKKNKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQD 168
           G Y++ +  V +P     ++  +N   +P  GA+GKQAVA R+S D A F  CR +GAQD
Sbjct: 165 GTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQD 224

Query: 169 TLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGF 228
           TLYDH+GRHY+KDCYIEGSVDF+FG+  S+Y+GCH+HAI +  GA+TAQ R+S+LE+TGF
Sbjct: 225 TLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGF 284

Query: 229 SFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRC 288
           SF+ CKV+G+G LYLGRAWG FSRVVFAYT+MD II P GWYNWGD +RE+TVFYGQY+C
Sbjct: 285 SFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKC 344

Query: 289 SGPGADYGGRVSWSRELTESEANPFLSLDFINANQWL 321
           +G GA+YGGRV+W+RELT+ EA PFLSL FI+ ++W+
Sbjct: 345 TGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

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BLAST of Lsi07G002290 vs. Swiss-Prot
Match: PME15_ARATH (Probable pectinesterase 15 OS=Arabidopsis thaliana GN=PME15 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-59
Identity = 106/207 (51.21%), Postives = 146/207 (70.53%), Query Frame = 1


Query: 126 KNKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDF 185
           KN A  P  G    QAVA RI GD A F  C F GAQDTL D  GRH+FK+C+I+GS+DF
Sbjct: 186 KNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDF 245

Query: 186 VFGDGRSIYDGCHLHAITNSY-----GALTAQKRNSLLEETGFSFLNCKVSGSGALYLGR 245
           +FG+GRS+Y  C +++I         G++TAQ R S  E++GFSF+NCK+ GSG + LGR
Sbjct: 246 IFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGR 305

Query: 246 AWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSREL 305
           AWG+++ VVF+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++L
Sbjct: 306 AWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQL 365

Query: 306 TESEANPFLSLDFINANQWLPNYHTHL 328
           T+SEA+ F+ + FI+ ++WL   HT++
Sbjct: 366 TDSEASSFIDVSFIDGDEWL--RHTNI 390

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BLAST of Lsi07G002290 vs. Swiss-Prot
Match: PME11_ARATH (Putative pectinesterase 11 OS=Arabidopsis thaliana GN=PME11 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.7e-58
Identity = 96/192 (50.00%), Postives = 131/192 (68.23%), Query Frame = 1


Query: 140 QAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHL 199
           +AVA R++ D A F  C     QDTL D  G HYFK+CYIEG+ DF+ G   S+Y+ CHL
Sbjct: 152 RAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL 211

Query: 200 HAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKII 259
           H+++ + G++TAQ R S  E++GF+FL CK++GSG+ +LGR WG++SRVVFAY+F   ++
Sbjct: 212 HSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVV 271

Query: 260 TPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDFINANQW 319
            P GW  WGD  +E TV+YG+Y+C GPGAD   RV WS++L++ EA  FLS DFI    W
Sbjct: 272 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDW 331

Query: 320 LPNYHTHLALKP 332
           L    +H    P
Sbjct: 332 LRPAPSHFKNAP 343

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BLAST of Lsi07G002290 vs. Swiss-Prot
Match: PME62_ARATH (Pectinesterase QRT1 OS=Arabidopsis thaliana GN=QRT1 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.3e-57
Identity = 96/187 (51.34%), Postives = 129/187 (68.98%), Query Frame = 1


Query: 134 SGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSI 193
           +G  G+QAVA RI GD A F   R +G+QDTL+D  G HYF  CYI+G+VDF+FG+ +S+
Sbjct: 192 AGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSL 251

Query: 194 YDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 253
           Y  C +H+    YGA+ A  R+S  E+TGFSF+NC +SG+G +YLGRAWG++SR V++  
Sbjct: 252 YQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNC 311

Query: 254 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDF 313
           F+  IITP GW +W    R+  V +G+Y C G GA+ GGRV WS+ LT  E  PFL  +F
Sbjct: 312 FIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREF 371

Query: 314 INANQWL 321
           I  +QWL
Sbjct: 372 IYGDQWL 378

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BLAST of Lsi07G002290 vs. Swiss-Prot
Match: PME68_ARATH (Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 100/195 (51.28%), Postives = 132/195 (67.69%), Query Frame = 1


Query: 127 NKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFV 186
           N A  P  G  G QAVAFRISGD A F  C F GAQDTL D  GRHYFK+CYIEGS+DF+
Sbjct: 163 NTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFI 222

Query: 187 FGDGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFS 246
           FG+GRS+Y  C LH+I + +G++ A  R    E+TGF+F+ C+V+G+G LY+GRA G +S
Sbjct: 223 FGNGRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYS 282

Query: 247 RVVFAYTFMDKIITPTGWYNWGDK-NRELTVFYGQYRCSGPGADYGGRVSWSRELTESEA 306
           R+V+AYT+ D ++   GW +W  K N+  T F+G Y C GPGA     VSW+R L    A
Sbjct: 283 RIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESA 342

Query: 307 NPFLSLDFINANQWL 321
           +PF++  F+N   W+
Sbjct: 343 HPFIAKSFVNGRHWI 357

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BLAST of Lsi07G002290 vs. TrEMBL
Match: A0A0A0KWM1_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G043950 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 5.9e-147
Identity = 275/379 (72.56%), Postives = 293/379 (77.31%), Query Frame = 1


Query: 4   PVMKLILFLLITIMLPGTALSYGQNWTVGSGGRGMR-TVEDEYLKWIRHMGSFKHSLFQK 63
           P++  + FLLI I+LP  A    QNWT G GGRGMR TV+DEY KWIRHMGSFKHSLFQ 
Sbjct: 4   PLIFNLFFLLIAIILPAAA----QNWTAG-GGRGMRSTVQDEYFKWIRHMGSFKHSLFQN 63

Query: 64  TKNKFKPCLTLKVSKNTKAGGFRSLQKAISSL--------------------PVINRCRV 123
           TKNKFKPCLTLKVSKNTKAGGFRSLQKA++SL                      I     
Sbjct: 64  TKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMS 123

Query: 124 LIRVAA------------------------GIYSTITLLVPTP-----SLQKKNKARLPP 183
            I V                          G +++ T  V +P     ++  KNKARLPP
Sbjct: 124 YIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPP 183

Query: 184 SGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSI 243
           SGALGKQAVAFRISGDAA FISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDG SI
Sbjct: 184 SGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSI 243

Query: 244 YDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 303
           YD CHLHAITNSYGALTAQKRNS+LEETGFSFL+CKVSGSGALYLGRAWGSFSRVVFAYT
Sbjct: 244 YDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYT 303

Query: 304 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDF 333
           FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELT+SEANPFLSLDF
Sbjct: 304 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDF 363

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BLAST of Lsi07G002290 vs. TrEMBL
Match: A0A067LQ67_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_06482 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.0e-114
Identity = 212/373 (56.84%), Postives = 262/373 (70.24%), Query Frame = 1


Query: 6   MKLILFLLITIMLPGTALSYGQNWTVGSGGRGMRTVEDE------YLKWIRH--MGSFKH 65
           MKL  F+L+ ++L     +        +  R + + E+E      YLKW++   +G FK+
Sbjct: 1   MKLFYFILLLLLLLFANANSLLTLNGSNSDRFLASEEEEEAEEEAYLKWLKEKQVGYFKN 60

Query: 66  SLFQKTKNKFKPCLTLKVSKNTKAGGFRSLQKAISSLPVINRCRV-----------LIRV 125
           SLF+K KN+FKPCLT+KV+K +K+GGF ++QKAI S+P++N CRV            + +
Sbjct: 61  SLFKKAKNRFKPCLTIKVNKKSKSGGFVTVQKAIDSIPIVNNCRVVISISAGTYREKVEI 120

Query: 126 AA---------------------------------GIYSTITLLVPTP-----SLQKKNK 185
            A                                 G Y + T  + +P     ++  KNK
Sbjct: 121 PASMAYITLEGDGTGKTIIEWDDTADKMGQGGHRLGTYGSATFAINSPYFIAKNITFKNK 180

Query: 186 ARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFG 245
           A  PPSGALGKQAVA RIS D A FI C FIGAQDTLYDH+GRHYFKDCYIEGSVDF+FG
Sbjct: 181 APSPPSGALGKQAVALRISADTAAFIGCNFIGAQDTLYDHIGRHYFKDCYIEGSVDFIFG 240

Query: 246 DGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRV 305
           +G S+Y+ CHLHAITNS+GALTAQKR+S+L+ETGFSF+NCKV+GSGALYLGRAWGSFSRV
Sbjct: 241 NGLSLYENCHLHAITNSFGALTAQKRDSMLQETGFSFVNCKVTGSGALYLGRAWGSFSRV 300

Query: 306 VFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPF 322
           VFAYT+MDKIITP GWYNWGDKNRE+TVFYGQY+CSGPGAD+GGRVSWSRELTE EA PF
Sbjct: 301 VFAYTYMDKIITPRGWYNWGDKNREMTVFYGQYKCSGPGADFGGRVSWSRELTEEEAKPF 360

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BLAST of Lsi07G002290 vs. TrEMBL
Match: A0A061DGR4_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_000251 PE=4 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 7.3e-113
Identity = 208/368 (56.52%), Postives = 253/368 (68.75%), Query Frame = 1


Query: 5   VMKLILFLLI--TIMLPGTALSYGQNWTVGSGGRGMRTVEDEYLKWIRHMGSFKHSLFQK 64
           +MKL LF+L+      P T+   G  + +         +E EY+ W+R M SFKHSLF +
Sbjct: 2   IMKLFLFILLLEAASFPVTSNRPGHEFVL---------IEKEYVNWVRQMSSFKHSLFGR 61

Query: 65  TKNKFKPCLTLKVSKNTKAGGFRSLQKAISSLPVINRCRV-----------LIRVAA--- 124
            KNK KPCL+++V+K  + G F ++QKAISSLPV+N CRV            + + A   
Sbjct: 62  AKNKLKPCLSIRVNKKPRLGQFATVQKAISSLPVVNNCRVVIYIGAGTYREKVEIPATMA 121

Query: 125 ------------------------------GIYSTITLLVPTP-----SLQKKNKARLPP 184
                                         G Y + T  V +P     ++  KNKA  PP
Sbjct: 122 YITLEGAGAGKTVIEWDDTADRMGQSGRPLGTYGSATFAVNSPYFIAKNITFKNKAPSPP 181

Query: 185 SGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSI 244
           SGALGKQAVA RIS D A FI C+FIGAQDTLYDH+GRHYFKDCYIEGSVDF+FG+G S+
Sbjct: 182 SGALGKQAVALRISADTAAFIGCKFIGAQDTLYDHIGRHYFKDCYIEGSVDFIFGNGLSL 241

Query: 245 YDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 304
           Y  CHLHA+TNSYGALTAQKR S LEETGFSF+NCKV+GSGAL+LGRAWG+FSRVVF YT
Sbjct: 242 YRDCHLHAVTNSYGALTAQKRESFLEETGFSFVNCKVTGSGALFLGRAWGTFSRVVFVYT 301

Query: 305 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDF 322
           +MDKIITP GWY+WGDKNRE+TVFYGQY+CSGPGA++GGRVSW+RELT+ EA PF+S+DF
Sbjct: 302 YMDKIITPRGWYDWGDKNREMTVFYGQYKCSGPGANFGGRVSWARELTQQEAKPFISVDF 360

[Close]

BLAST of Lsi07G002290 vs. TrEMBL
Match: B9SZT7_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_0291660 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 6.9e-111
Identity = 212/368 (57.61%), Postives = 254/368 (69.02%), Query Frame = 1


Query: 6   MKLILFLLITIMLPGTALSYGQNWTVGSGGRGMRTV--EDEYLKWIR--HMGSFKHSLFQ 65
           M L+  LL  I+L G+A S    W   +G    R V  E+ YL+W++   MGSFKH+LFQ
Sbjct: 1   MLLLFKLLCLILLSGSATS----WFTSNGSNSNRFVVNEEAYLQWLKAKQMGSFKHALFQ 60

Query: 66  KTKNKFKPCLTLKVSKNTKAGGFRSLQKAIS-----------------------SLPVIN 125
           K KNKFKPCLT+KV+K +K+G F +L+KA++                        +P   
Sbjct: 61  KAKNKFKPCLTIKVNKKSKSGDFVTLKKALNSIPVINNCRVIISIGAGTYREKIEIPGSM 120

Query: 126 RCRVLIRVAAG---------------------IYSTITLLVPTP-----SLQKKNKARLP 185
               L    AG                      Y + T  + +P     ++  KNKA  P
Sbjct: 121 SYITLEGAGAGKTTIEWDDTADKQGQGGHLLGTYGSATFAINSPYFIAKNITFKNKAPSP 180

Query: 186 PSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRS 245
           PSGALGKQAVA RIS D A FI C+FIGAQDTLYDH+GRHYFK+CYIEGSVDF+FG+G S
Sbjct: 181 PSGALGKQAVALRISADTAAFIGCKFIGAQDTLYDHIGRHYFKECYIEGSVDFIFGNGLS 240

Query: 246 IYDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAY 305
           +YD CHLHAITNS+GALTAQKR S+LEETGFSF+NCKV+GSGALYLGRAWG+FSRVVFAY
Sbjct: 241 LYDDCHLHAITNSFGALTAQKRESMLEETGFSFVNCKVTGSGALYLGRAWGTFSRVVFAY 300

Query: 306 TFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLD 321
           T+MDKIITPTGWY+WGDKNRE+TVFYGQY+CSGPGA +GGRVSWSRELTE EA PF+S+D
Sbjct: 301 TYMDKIITPTGWYDWGDKNREMTVFYGQYKCSGPGAQFGGRVSWSRELTEQEAKPFVSID 360

[Close]

BLAST of Lsi07G002290 vs. TrEMBL
Match: W9T0W5_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_026720 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.2e-110
Identity = 204/362 (56.35%), Postives = 251/362 (69.34%), Query Frame = 1


Query: 9   ILFLLITIMLPGTALSYGQNWTVGSGGRGMRTVEDEYLKWIRHMGSFKHSLFQKTKNKFK 68
           +LFLL+  ++  +  S        +   G+ TVE+ YL W++ M S+KHSLFQK  NKF 
Sbjct: 6   VLFLLVIFLVFKSVGS-------STSPNGLETVEEAYLNWVKQMDSYKHSLFQKAINKFH 65

Query: 69  PCLTLKVSKNTKAGGFRSLQKAISSLPVINRCRVLIRV---------------------A 128
           PC T+KV+KN + G F SLQKAI+SLP  + CRV+I +                      
Sbjct: 66  PCKTIKVNKNPRLGAFTSLQKAINSLPTFSNCRVVISLGAGIYREKVEIPNTMAYITLEG 125

Query: 129 AGIYSTI-----------------------TLLVPTP-----SLQKKNKARLPPSGALGK 188
           +G++ TI                       T  V +P     ++  KNKA  PPSGALGK
Sbjct: 126 SGVHKTIIEWDDTADRLGQSGRPLGTFGSATFAVNSPYFIAKNVTFKNKAPSPPSGALGK 185

Query: 189 QAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHL 248
           QAVA RIS D A FISC+FIG+QDTLYDH+GRHYF++CYIEGSVDF+FG+G S+Y GCHL
Sbjct: 186 QAVALRISADTAAFISCKFIGSQDTLYDHIGRHYFENCYIEGSVDFIFGNGLSLYRGCHL 245

Query: 249 HAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKII 308
           HA  NSYGALTAQKR +LL+ETGFSF+ CKV+GSGALYLGRAWG+FSRVVFA+T+MDKI+
Sbjct: 246 HATGNSYGALTAQKRENLLQETGFSFVKCKVTGSGALYLGRAWGTFSRVVFAFTYMDKIV 305

Query: 309 TPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDFINANQW 322
           TP GWY+WGDKNR++TVFYGQY+C GPGA +GGRVSWSRELT  EA PF+SL FIN  +W
Sbjct: 306 TPRGWYDWGDKNRDMTVFYGQYKCMGPGAGFGGRVSWSRELTPQEARPFISLGFINGYEW 360

[Close]

BLAST of Lsi07G002290 vs. TAIR10
Match: AT5G19730.1 (AT5G19730.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 310.1 bits (793), Expect = 1.7e-84
Identity = 136/217 (62.67%), Postives = 175/217 (80.65%), Query Frame = 1


Query: 109 GIYSTITLLVPTP-----SLQKKNKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQD 168
           G Y++ +  V +P     ++  +N   +P  GA+GKQAVA R+S D A F  CR +GAQD
Sbjct: 165 GTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQD 224

Query: 169 TLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGF 228
           TLYDH+GRHY+KDCYIEGSVDF+FG+  S+Y+GCH+HAI +  GA+TAQ R+S+LE+TGF
Sbjct: 225 TLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGF 284

Query: 229 SFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRC 288
           SF+ CKV+G+G LYLGRAWG FSRVVFAYT+MD II P GWYNWGD +RE+TVFYGQY+C
Sbjct: 285 SFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKC 344

Query: 289 SGPGADYGGRVSWSRELTESEANPFLSLDFINANQWL 321
           +G GA+YGGRV+W+RELT+ EA PFLSL FI+ ++W+
Sbjct: 345 TGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

[Close]

BLAST of Lsi07G002290 vs. TAIR10
Match: AT2G36710.1 (AT2G36710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 231.9 bits (590), Expect = 5.8e-61
Identity = 106/207 (51.21%), Postives = 146/207 (70.53%), Query Frame = 1


Query: 126 KNKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDF 185
           KN A  P  G    QAVA RI GD A F  C F GAQDTL D  GRH+FK+C+I+GS+DF
Sbjct: 186 KNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDF 245

Query: 186 VFGDGRSIYDGCHLHAITNSY-----GALTAQKRNSLLEETGFSFLNCKVSGSGALYLGR 245
           +FG+GRS+Y  C +++I         G++TAQ R S  E++GFSF+NCK+ GSG + LGR
Sbjct: 246 IFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGR 305

Query: 246 AWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSREL 305
           AWG+++ VVF+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++L
Sbjct: 306 AWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQL 365

Query: 306 TESEANPFLSLDFINANQWLPNYHTHL 328
           T+SEA+ F+ + FI+ ++WL   HT++
Sbjct: 366 TDSEASSFIDVSFIDGDEWL--RHTNI 390

[Close]

BLAST of Lsi07G002290 vs. TAIR10
Match: AT2G21610.1 (AT2G21610.1 pectinesterase 11)

HSP 1 Score: 225.3 bits (573), Expect = 5.4e-59
Identity = 96/192 (50.00%), Postives = 131/192 (68.23%), Query Frame = 1


Query: 140 QAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHL 199
           +AVA R++ D A F  C     QDTL D  G HYFK+CYIEG+ DF+ G   S+Y+ CHL
Sbjct: 152 RAVALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL 211

Query: 200 HAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKII 259
           H+++ + G++TAQ R S  E++GF+FL CK++GSG+ +LGR WG++SRVVFAY+F   ++
Sbjct: 212 HSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVV 271

Query: 260 TPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDFINANQW 319
            P GW  WGD  +E TV+YG+Y+C GPGAD   RV WS++L++ EA  FLS DFI    W
Sbjct: 272 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDW 331

Query: 320 LPNYHTHLALKP 332
           L    +H    P
Sbjct: 332 LRPAPSHFKNAP 343

[Close]

BLAST of Lsi07G002290 vs. TAIR10
Match: AT5G55590.1 (AT5G55590.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 222.6 bits (566), Expect = 3.5e-58
Identity = 96/187 (51.34%), Postives = 129/187 (68.98%), Query Frame = 1


Query: 134 SGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSI 193
           +G  G+QAVA RI GD A F   R +G+QDTL+D  G HYF  CYI+G+VDF+FG+ +S+
Sbjct: 192 AGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSL 251

Query: 194 YDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 253
           Y  C +H+    YGA+ A  R+S  E+TGFSF+NC +SG+G +YLGRAWG++SR V++  
Sbjct: 252 YQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNC 311

Query: 254 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDF 313
           F+  IITP GW +W    R+  V +G+Y C G GA+ GGRV WS+ LT  E  PFL  +F
Sbjct: 312 FIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREF 371

Query: 314 INANQWL 321
           I  +QWL
Sbjct: 372 IYGDQWL 378

[Close]

BLAST of Lsi07G002290 vs. TAIR10
Match: AT5G47500.1 (AT5G47500.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 220.7 bits (561), Expect = 1.3e-57
Identity = 100/195 (51.28%), Postives = 132/195 (67.69%), Query Frame = 1


Query: 127 NKARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFV 186
           N A  P  G  G QAVAFRISGD A F  C F GAQDTL D  GRHYFK+CYIEGS+DF+
Sbjct: 163 NTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFI 222

Query: 187 FGDGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFS 246
           FG+GRS+Y  C LH+I + +G++ A  R    E+TGF+F+ C+V+G+G LY+GRA G +S
Sbjct: 223 FGNGRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYS 282

Query: 247 RVVFAYTFMDKIITPTGWYNWGDK-NRELTVFYGQYRCSGPGADYGGRVSWSRELTESEA 306
           R+V+AYT+ D ++   GW +W  K N+  T F+G Y C GPGA     VSW+R L    A
Sbjct: 283 RIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESA 342

Query: 307 NPFLSLDFINANQWL 321
           +PF++  F+N   W+
Sbjct: 343 HPFIAKSFVNGRHWI 357

[Close]

BLAST of Lsi07G002290 vs. NCBI nr
Match: gi|659130584|ref|XP_008465244.1| (PREDICTED: probable pectinesterase 53 [Cucumis melo])

HSP 1 Score: 528.9 bits (1361), Expect = 6.5e-147
Identity = 274/378 (72.49%), Postives = 292/378 (77.25%), Query Frame = 1


Query: 4   PVMKLILFLLITIMLPGTALSYGQNWTVGSGGRGMRTVEDEYLKWIRHMGSFKHSLFQKT 63
           P++  +LFLLI I+LP  A    QNWT G GGRGMRTV+DEYLKWIRHMGSFKHSLFQ T
Sbjct: 3   PLIFNLLFLLIAIILPAAA----QNWTAG-GGRGMRTVQDEYLKWIRHMGSFKHSLFQNT 62

Query: 64  KNKFKPCLTLKVSKNTKAGGFRSLQKAISSL--------------------PVINRCRVL 123
           KNKFKPCLTLKVSKN K GGFRSLQKA++SL                      I      
Sbjct: 63  KNKFKPCLTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSY 122

Query: 124 IRVAA------------------------GIYSTITLLVPTP-----SLQKKNKARLPPS 183
           I V                          G +++ T  V +P     ++  KNKARLPPS
Sbjct: 123 IWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPS 182

Query: 184 GALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSIY 243
           GALGKQAVAFRISGDAA FISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDG SIY
Sbjct: 183 GALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 242

Query: 244 DGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTF 303
           D CHLHAITNSYGALTAQKRNS+LEETGFSFL+CKVSGSGALYLGRAWGSFSRVVFAYTF
Sbjct: 243 DSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTF 302

Query: 304 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDFI 333
           MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELT+SEANPFLSLDFI
Sbjct: 303 MDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFI 362

[Close]

BLAST of Lsi07G002290 vs. NCBI nr
Match: gi|449457504|ref|XP_004146488.1| (PREDICTED: probable pectinesterase 53 [Cucumis sativus])

HSP 1 Score: 528.5 bits (1360), Expect = 8.5e-147
Identity = 275/379 (72.56%), Postives = 293/379 (77.31%), Query Frame = 1


Query: 4   PVMKLILFLLITIMLPGTALSYGQNWTVGSGGRGMR-TVEDEYLKWIRHMGSFKHSLFQK 63
           P++  + FLLI I+LP  A    QNWT G GGRGMR TV+DEY KWIRHMGSFKHSLFQ 
Sbjct: 4   PLIFNLFFLLIAIILPAAA----QNWTAG-GGRGMRSTVQDEYFKWIRHMGSFKHSLFQN 63

Query: 64  TKNKFKPCLTLKVSKNTKAGGFRSLQKAISSL--------------------PVINRCRV 123
           TKNKFKPCLTLKVSKNTKAGGFRSLQKA++SL                      I     
Sbjct: 64  TKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMS 123

Query: 124 LIRVAA------------------------GIYSTITLLVPTP-----SLQKKNKARLPP 183
            I V                          G +++ T  V +P     ++  KNKARLPP
Sbjct: 124 YIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPP 183

Query: 184 SGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSI 243
           SGALGKQAVAFRISGDAA FISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDG SI
Sbjct: 184 SGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSI 243

Query: 244 YDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 303
           YD CHLHAITNSYGALTAQKRNS+LEETGFSFL+CKVSGSGALYLGRAWGSFSRVVFAYT
Sbjct: 244 YDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYT 303

Query: 304 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDF 333
           FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELT+SEANPFLSLDF
Sbjct: 304 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDF 363

[Close]

BLAST of Lsi07G002290 vs. NCBI nr
Match: gi|802537509|ref|XP_012065299.1| (PREDICTED: probable pectinesterase 53 [Jatropha curcas])

HSP 1 Score: 421.4 bits (1082), Expect = 1.5e-114
Identity = 212/373 (56.84%), Postives = 262/373 (70.24%), Query Frame = 1


Query: 6   MKLILFLLITIMLPGTALSYGQNWTVGSGGRGMRTVEDE------YLKWIRH--MGSFKH 65
           MKL  F+L+ ++L     +        +  R + + E+E      YLKW++   +G FK+
Sbjct: 1   MKLFYFILLLLLLLFANANSLLTLNGSNSDRFLASEEEEEAEEEAYLKWLKEKQVGYFKN 60

Query: 66  SLFQKTKNKFKPCLTLKVSKNTKAGGFRSLQKAISSLPVINRCRV-----------LIRV 125
           SLF+K KN+FKPCLT+KV+K +K+GGF ++QKAI S+P++N CRV            + +
Sbjct: 61  SLFKKAKNRFKPCLTIKVNKKSKSGGFVTVQKAIDSIPIVNNCRVVISISAGTYREKVEI 120

Query: 126 AA---------------------------------GIYSTITLLVPTP-----SLQKKNK 185
            A                                 G Y + T  + +P     ++  KNK
Sbjct: 121 PASMAYITLEGDGTGKTIIEWDDTADKMGQGGHRLGTYGSATFAINSPYFIAKNITFKNK 180

Query: 186 ARLPPSGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFG 245
           A  PPSGALGKQAVA RIS D A FI C FIGAQDTLYDH+GRHYFKDCYIEGSVDF+FG
Sbjct: 181 APSPPSGALGKQAVALRISADTAAFIGCNFIGAQDTLYDHIGRHYFKDCYIEGSVDFIFG 240

Query: 246 DGRSIYDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRV 305
           +G S+Y+ CHLHAITNS+GALTAQKR+S+L+ETGFSF+NCKV+GSGALYLGRAWGSFSRV
Sbjct: 241 NGLSLYENCHLHAITNSFGALTAQKRDSMLQETGFSFVNCKVTGSGALYLGRAWGSFSRV 300

Query: 306 VFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPF 322
           VFAYT+MDKIITP GWYNWGDKNRE+TVFYGQY+CSGPGAD+GGRVSWSRELTE EA PF
Sbjct: 301 VFAYTYMDKIITPRGWYNWGDKNREMTVFYGQYKCSGPGADFGGRVSWSRELTEEEAKPF 360

[Close]

BLAST of Lsi07G002290 vs. NCBI nr
Match: gi|590702983|ref|XP_007046753.1| (Pectin lyase-like superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 415.2 bits (1066), Expect = 1.1e-112
Identity = 208/368 (56.52%), Postives = 253/368 (68.75%), Query Frame = 1


Query: 5   VMKLILFLLI--TIMLPGTALSYGQNWTVGSGGRGMRTVEDEYLKWIRHMGSFKHSLFQK 64
           +MKL LF+L+      P T+   G  + +         +E EY+ W+R M SFKHSLF +
Sbjct: 2   IMKLFLFILLLEAASFPVTSNRPGHEFVL---------IEKEYVNWVRQMSSFKHSLFGR 61

Query: 65  TKNKFKPCLTLKVSKNTKAGGFRSLQKAISSLPVINRCRV-----------LIRVAA--- 124
            KNK KPCL+++V+K  + G F ++QKAISSLPV+N CRV            + + A   
Sbjct: 62  AKNKLKPCLSIRVNKKPRLGQFATVQKAISSLPVVNNCRVVIYIGAGTYREKVEIPATMA 121

Query: 125 ------------------------------GIYSTITLLVPTP-----SLQKKNKARLPP 184
                                         G Y + T  V +P     ++  KNKA  PP
Sbjct: 122 YITLEGAGAGKTVIEWDDTADRMGQSGRPLGTYGSATFAVNSPYFIAKNITFKNKAPSPP 181

Query: 185 SGALGKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSI 244
           SGALGKQAVA RIS D A FI C+FIGAQDTLYDH+GRHYFKDCYIEGSVDF+FG+G S+
Sbjct: 182 SGALGKQAVALRISADTAAFIGCKFIGAQDTLYDHIGRHYFKDCYIEGSVDFIFGNGLSL 241

Query: 245 YDGCHLHAITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYT 304
           Y  CHLHA+TNSYGALTAQKR S LEETGFSF+NCKV+GSGAL+LGRAWG+FSRVVF YT
Sbjct: 242 YRDCHLHAVTNSYGALTAQKRESFLEETGFSFVNCKVTGSGALFLGRAWGTFSRVVFVYT 301

Query: 305 FMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDF 322
           +MDKIITP GWY+WGDKNRE+TVFYGQY+CSGPGA++GGRVSW+RELT+ EA PF+S+DF
Sbjct: 302 YMDKIITPRGWYDWGDKNREMTVFYGQYKCSGPGANFGGRVSWARELTQQEAKPFISVDF 360

[Close]

BLAST of Lsi07G002290 vs. NCBI nr
Match: gi|720049550|ref|XP_010271483.1| (PREDICTED: probable pectinesterase 53 [Nelumbo nucifera])

HSP 1 Score: 413.7 bits (1062), Expect = 3.1e-112
Identity = 211/361 (58.45%), Postives = 251/361 (69.53%), Query Frame = 1


Query: 10  LFLLITIMLPGTALSYGQNWTVGSGGRGMRTVEDEYLKWIRHMGSFKHSLFQKTKNKFKP 69
           LFLLI ++  G   S   N     G  G   +++ YL WI+ MGS  HSL+ K +NK KP
Sbjct: 41  LFLLI-LLFEGAPSSVSLN-----GTNGTVIIQEGYLNWIKQMGSLTHSLYNKAENKLKP 100

Query: 70  CLTLKVSKNTKAGGFRSLQKAISSLPV-------------INRCRVLI------------ 129
           CLT+ V K+ K G F ++QKA+ SLP+             I R +V I            
Sbjct: 101 CLTITVDKDPKKGDFVTVQKAVDSLPIVNQCRVLIKIHAGIYREKVDIPATKAFVTLEGA 160

Query: 130 -------------------RVAAGIYSTITLLVPTP-----SLQKKNKARLPPSGALGKQ 189
                              R   G +++ T  + +P     ++  KN A LPPSGALGKQ
Sbjct: 161 GADRTILQWGDTADRMGSDRRPLGTFASATFAINSPYFVAKNITFKNTAPLPPSGALGKQ 220

Query: 190 AVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGRSIYDGCHLH 249
           AVA RISGD A F SC FIGAQDTLYDH+GRHYFKDCYI+GSVDF+FGDG S+Y+GCHLH
Sbjct: 221 AVALRISGDTAAFFSCNFIGAQDTLYDHIGRHYFKDCYIQGSVDFIFGDGLSLYEGCHLH 280

Query: 250 AITNSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIIT 309
           AITNSYGALTAQKR+++LEETGFSF+NC+V+GSGALYLGRAWGSFSRVVFAYT+MDKIIT
Sbjct: 281 AITNSYGALTAQKRDNMLEETGFSFVNCRVTGSGALYLGRAWGSFSRVVFAYTYMDKIIT 340

Query: 310 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTESEANPFLSLDFINANQWL 322
           P GWYNWGDKNRELTVFYGQY+CSGPGAD+GGRVSWSRELT+ EA PF+SL FI+  +WL
Sbjct: 341 PKGWYNWGDKNRELTVFYGQYKCSGPGADFGGRVSWSRELTQQEAKPFISLSFIDGYEWL 395

The following BLAST results are available for this feature:

BLAST of Lsi07G002290 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME53_ARATH	3.0e-83	62.67	Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1	[more]
PME15_ARATH	1.0e-59	51.21	Probable pectinesterase 15 OS=Arabidopsis thaliana GN=PME15 PE=2 SV=1	[more]
PME11_ARATH	9.7e-58	50.00	Putative pectinesterase 11 OS=Arabidopsis thaliana GN=PME11 PE=3 SV=1	[more]
PME62_ARATH	6.3e-57	51.34	Pectinesterase QRT1 OS=Arabidopsis thaliana GN=QRT1 PE=2 SV=1	[more]
PME68_ARATH	2.4e-56	51.28	Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1	[more]

BLAST of Lsi07G002290 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWM1_CUCSA	5.9e-147	72.56	Pectinesterase OS=Cucumis sativus GN=Csa_4G043950 PE=4 SV=1	[more]
A0A067LQ67_JATCU	1.0e-114	56.84	Pectinesterase OS=Jatropha curcas GN=JCGZ_06482 PE=4 SV=1	[more]
A0A061DGR4_THECC	7.3e-113	56.52	Pectinesterase OS=Theobroma cacao GN=TCM_000251 PE=4 SV=1	[more]
B9SZT7_RICCO	6.9e-111	57.61	Pectinesterase OS=Ricinus communis GN=RCOM_0291660 PE=4 SV=1	[more]
W9T0W5_9ROSA	1.2e-110	56.35	Pectinesterase OS=Morus notabilis GN=L484_026720 PE=4 SV=1	[more]

BLAST of Lsi07G002290 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19730.1	1.7e-84	62.67	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	5.8e-61	51.21	Pectin lyase-like superfamily protein	[more]
AT2G21610.1	5.4e-59	50.00	pectinesterase 11	[more]
AT5G55590.1	3.5e-58	51.34	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	1.3e-57	51.28	Pectin lyase-like superfamily protein	[more]

BLAST of Lsi07G002290 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659130584\|ref\|XP_008465244.1\|	6.5e-147	72.49	PREDICTED: probable pectinesterase 53 [Cucumis melo]	[more]
gi\|449457504\|ref\|XP_004146488.1\|	8.5e-147	72.56	PREDICTED: probable pectinesterase 53 [Cucumis sativus]	[more]
gi\|802537509\|ref\|XP_012065299.1\|	1.5e-114	56.84	PREDICTED: probable pectinesterase 53 [Jatropha curcas]	[more]
gi\|590702983\|ref\|XP_007046753.1\|	1.1e-112	56.52	Pectin lyase-like superfamily protein isoform 1 [Theobroma cacao]	[more]
gi\|720049550\|ref\|XP_010271483.1\|	3.1e-112	58.45	PREDICTED: probable pectinesterase 53 [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **INTERPRO**
Term	Definition
IPR012334	Pectin_lyas_fold
IPR011050	Pectin_lyase_fold/virulence
IPR000070	Pectinesterase_cat

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005618	cell wall
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0016829	lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G002290.1	Lsi07G002290.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 134..316 score: 1.4
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 72..326 score: 1.67
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 134..324 score: 1.9E-70coord: 73..113 score: 1.
None	No IPR available	PANTHER	PTHR31321	FAMILY NOT NAMED	coord: 42..320 score: 9.6E
None	No IPR available	PANTHER	PTHR31321:SF25	SUBFAMILY NOT NAMED	coord: 42..320 score: 9.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi07G002290	Csa4G043950	Cucumber (Chinese Long) v2	culsiB303
Lsi07G002290	CSPI04G05200	Wild cucumber (PI 183967)	cpilsiB303
Lsi07G002290	Cucsa.201150	Cucumber (Gy14) v1	cgylsiB344
Lsi07G002290	CmaCh04G007700	Cucurbita maxima (Rimu)	cmalsiB698
Lsi07G002290	CmoCh04G008230	Cucurbita moschata (Rifu)	cmolsiB692
Lsi07G002290	Cla014557	Watermelon (97103) v1	lsiwmB439
Lsi07G002290	ClCG07G008840	Watermelon (Charleston Gray)	lsiwcgB413
Lsi07G002290	Cla97C07G135870	Watermelon (97103) v2	lsiwmbB412
Lsi07G002290	Bhi01G001887	Wax gourd	lsiwgoB555
Lsi07G002290	Cp4.1LG01g06800	Cucurbita pepo (Zucchini)	cpelsiB373
Lsi07G002290	CsGy4G005120	Cucumber (Gy14) v2	cgyblsiB277
Lsi07G002290	Carg14169	Silver-seed gourd	carlsiB859
Lsi07G002290	CsaV3_4G005340	Cucumber (Chinese Long) v3	cuclsiB322

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi07G002290	Wax gourd	lsiwgoB570
Lsi07G002290	Bottle gourd (USVL1VR-Ls)	lsilsiB082
Lsi07G002290	Cucumber (Gy14) v1	cgylsiB027
Lsi07G002290	Cucurbita maxima (Rimu)	cmalsiB326
Lsi07G002290	Cucurbita maxima (Rimu)	cmalsiB618
Lsi07G002290	Cucurbita maxima (Rimu)	cmalsiB806
Lsi07G002290	Cucurbita moschata (Rifu)	cmolsiB315
Lsi07G002290	Cucurbita moschata (Rifu)	cmolsiB606
Lsi07G002290	Cucurbita moschata (Rifu)	cmolsiB792
Lsi07G002290	Cucurbita pepo (Zucchini)	cpelsiB185
Lsi07G002290	Wild cucumber (PI 183967)	cpilsiB477
Lsi07G002290	Cucumber (Chinese Long) v2	culsiB469
Lsi07G002290	Melon (DHL92) v3.5.1	lsimeB454
Lsi07G002290	Melon (DHL92) v3.5.1	lsimeB460
Lsi07G002290	Watermelon (Charleston Gray)	lsiwcgB396
Lsi07G002290	Watermelon (97103) v1	lsiwmB455
Lsi07G002290	Cucumber (Gy14) v2	cgyblsiB435
Lsi07G002290	Melon (DHL92) v3.6.1	lsimedB448
Lsi07G002290	Melon (DHL92) v3.6.1	lsimedB471
Lsi07G002290	Melon (DHL92) v3.6.1	lsimedB478
Lsi07G002290	Silver-seed gourd	carlsiB369
Lsi07G002290	Silver-seed gourd	carlsiB935
Lsi07G002290	Cucumber (Chinese Long) v3	cuclsiB151
Lsi07G002290	Cucumber (Chinese Long) v3	cuclsiB499
Lsi07G002290	Watermelon (97103) v2	lsiwmbB393