CSPI04G05200 (gene) Wild cucumber (PI 183967)

NameCSPI04G05200
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPectinesterase
LocationChr4 : 3525246 .. 3528505 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAGACAATTTGTATACAAACATACACACACAGCCTATCTCCAAGATTCCCCAATATTGTTCAAACCCTTCTCATTCTCTCAAAATCACTTCATTTTTTATCTACACATATTTCCCAATAATCAAACACACCAGAGGTTTTTCATTTAATTTCTTGAACAAACACATGAGTAGGCCGTTGATATTTAACCTTTTCTTTCTCCTCATCGCCATCATCCTCCCGGCCGCCGCCCAAAACTGGACGGCTGGTGGCGGCCGGGGAATGAGATCAACAGTGCAAGATGAATACTTCAAGTGGATAAGACATATGGGTTCTTTCAAGCACTCTCTTTTCCAAAACACTAAGAACAAGTTCAAGCCTTGTTTGACGTTGAAGGTTTCCAAGAACACCAAGGCTGGTGGTTTCCGCTCGCTTCAGAAGGCGGTGAACTCTTTGCCGATTATCAACCGGTGTCGGGTCCGTATCCATGTTGCTGCTGGAATCTACAGGTCCGTTTTTTTATTTTTTTTAACTTTCTTGTATGAGAATGTCCATATCTAATAATTATTAAGTTGTACTTATCTTTTTAACTATTGTTGTGTATTTAAAGAGAGAAAGTTGAGATTCCAGCGACGATGAGTTACATATGGGTGGAAGGTGAAGGAGCGGAGAAGACAATCATTGAATGGGGCGACACGGCGGACCATATGGGAGAAAATGGCCGTCCTATGGGCACTTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCATTCTTCATTGCTACTAACATCACCTTCAAGGTATTAACTTTTTCTTCAATTCTTTCTTTCCATCCCTTTGCCTTTGAAATTTGTCTTTTAATCTAATTTTTGAAAAAGTTTTGGTATCATCTTTTTTACTTTTTATAAACGGTTTATGACTACATTTAGAAATTTAAAAATAATGGTTAGAATGTGTAATGAAAATTAGAATCTGAAATGGTGTGATATACTCATTGTCACAAACTAAAATTGAGACTTTAATATCCTCTTTTGGTGTCTAAATTTCAATTCCATCCTTTTTTTTTCCAATTAATTGGTATCGAAACAGAGAGTTCATCCGACACTAATAATAATTTTGTTATATTTTATAAATATTTTGGATTATTTAATTAAATTTGAAAATGTCATTTTAAAAATAAAATTAAATATTGTTTTTTAAATCTACTCGTAACTTGCAAGTACTTTTTAGATTTAGATTCTACTTTTACTAGGATTATATATATAACTTCAACTTGTCATATTTTATTTAAAAAAAAAAACAAAAAGAACTTATCATATTTTATTACGAAATTGAAATAAATTAATTTTCTTAGAAGCACATACTTTTTAAATATATATGAGTAATCAACTTTTAAGTGAGAAAAATGGATCAATGGTTTGTATTATAATTAATTAGTAGCGCAATTGCTATTTTGAATTTTTAGGAGACTAAAATGAGATTAGTTGAAGAATAATTTAATAAGATTTGTGAGTTTTTTTAGAAAGTATATTATATATCATAATCCATTGAAAATTTCCAAAATATAAAAGGAGTTAAGTTAGGTTTAAATTTGAGGGTGTACATATAGTTTTATTTATGCTTAAAAGTTAAAATGAAGAATATATAGAAGGAATAAAGTTGAAGTGTCGTATGTAAATGGCAATTTGGTTGTGTGTTTCGATTGACCAAACTGACATCTTTGTTTTCTTTATTTTTCTTTTAATACAAAGACCGAACCGACATGTAGTTCTTGGAAGGACTTGATCCATAATTAAAATAATATCATTATTAGTAATTAAGTTTTAATTTAAGAATTAATAAATTAATATTTATTCAAATATTGTTCTCATTCTTATTATATTTTAATATATCCCTGCTAAAAATTGACTGTTCATTGGAAGTAGGGTTAATTTTTTATTTATATTCTACCAAATTTAAAAATTGTATTCAAGAAAACGAAACAGCTATCCATCGTCACCTCAAATTTCTTGCTTTACTATCCATCTTTAAAACTTTCAAAACATTATGTTTTATCTCTTCATTTTTCTTGCAACTAGCAATAAATAATTTTAAAGAAAATTACATTTATTTTTGTTTGAAATTACGCCAATATTCTTAAATCACTAATTGACCCAAATGTTTAAATTTATAGGTGAAGACAATTTAATATAATGTCCAGCACTTTCAATCATTTATGAGTTTGAAATGTGAAAATGTCCTATATAATACTAATTGGGGAGAAAATAACGCAATGACTCACACATGACATGCTAGGATCAAACTGCTATGACGCCACTTTAAATCACTAATTGATTCAAAAGCGTAAGTTAGTAGATGAAGACAAATTTAATATAATATGTAACAAATATATTGTTTTACGTGATGCCTCATTTTTTTTTTTTTTTAAAAAAGAACAAGGCGAGGCTTCCACCATCAGGAGCACTGGGAAAGCAAGCAGTTGCATTCAGAATATCAGGAGATGCAGCAGCATTTATAAGTTGTAGGTTTATCGGTGCACAAGACACATTATACGACCATATGGGGCGACACTACTTCAAGGATTGCTACATTGAAGGGTCAGTGGACTTCGTGTTCGGTGATGGCCTCTCCATCTATGATAGTTGCCATTTGCATGCCATCACCAACAGTTATGGTGCTCTCACGGCCCAAAAGAGGAATAGCATGCTCGAAGAAACTGGCTTCTCTTTCCTTCATTGTAAAGTTTCTGGCTCAGGCGCCTTGTATTTGGGTAGGGCATGGGGCAGCTTCTCTAGAGTTGTGTTTGCTTATACATTTATGGATAAAATCATCACTCCCACTGGTTGGTACAACTGGGGCGACAAAAATCGTGAATTGTAAGTATTTCTTTTAGATACTATTGCAATATTTCAGAATATATCAAATAGTAGATAACTGATAAACTTTTATTAACGTAGGACTGTATTTTATGGACAATATCGATGCTCGGGACCGGGAGCTGATTATGGAGGAAGGGTTCCATGGTCTAGAGAACTCACTCAGTCAGAAGCCAACCCATTTCTTTCTCTTGATTTTATCAATGCAAACCAATGGCTTCCAAATTACCACACTCATCTCTCTCTTAAACCACATTGATTCTGTCCTTTTCTCATTTCAAATTATTTTATTTTATTTTCTTAATTATGTATTATGACCTCAATATTCTAATTTTTTGTTTGTCCAATTATATTGGTAGGTTCTTTGATTTTTTTAATCATGTTTAAAATAAAATCCATTTATC

mRNA sequence

ATGAGTAGGCCGTTGATATTTAACCTTTTCTTTCTCCTCATCGCCATCATCCTCCCGGCCGCCGCCCAAAACTGGACGGCTGGTGGCGGCCGGGGAATGAGATCAACAGTGCAAGATGAATACTTCAAGTGGATAAGACATATGGGTTCTTTCAAGCACTCTCTTTTCCAAAACACTAAGAACAAGTTCAAGCCTTGTTTGACGTTGAAGGTTTCCAAGAACACCAAGGCTGGTGGTTTCCGCTCGCTTCAGAAGGCGGTGAACTCTTTGCCGATTATCAACCGGTGTCGGGTCCGTATCCATGTTGCTGCTGGAATCTACAGAGAGAAAGTTGAGATTCCAGCGACGATGAGTTACATATGGGTGGAAGGTGAAGGAGCGGAGAAGACAATCATTGAATGGGGCGACACGGCGGACCATATGGGAGAAAATGGCCGTCCTATGGGCACTTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCATTCTTCATTGCTACTAACATCACCTTCAAGAACAAGGCGAGGCTTCCACCATCAGGAGCACTGGGAAAGCAAGCAGTTGCATTCAGAATATCAGGAGATGCAGCAGCATTTATAAGTTGTAGGTTTATCGGTGCACAAGACACATTATACGACCATATGGGGCGACACTACTTCAAGGATTGCTACATTGAAGGGTCAGTGGACTTCGTGTTCGGTGATGGCCTCTCCATCTATGATAGTTGCCATTTGCATGCCATCACCAACAGTTATGGTGCTCTCACGGCCCAAAAGAGGAATAGCATGCTCGAAGAAACTGGCTTCTCTTTCCTTCATTGTAAAGTTTCTGGCTCAGGCGCCTTGTATTTGGGTAGGGCATGGGGCAGCTTCTCTAGAGTTGTGTTTGCTTATACATTTATGGATAAAATCATCACTCCCACTGGTTGGTACAACTGGGGCGACAAAAATCGTGAATTGACTGTATTTTATGGACAATATCGATGCTCGGGACCGGGAGCTGATTATGGAGGAAGGGTTCCATGGTCTAGAGAACTCACTCAGTCAGAAGCCAACCCATTTCTTTCTCTTGATTTTATCAATGCAAACCAATGGCTTCCAAATTACCACACTCATCTCTCTCTTAAACCACATTGA

Coding sequence (CDS)

ATGAGTAGGCCGTTGATATTTAACCTTTTCTTTCTCCTCATCGCCATCATCCTCCCGGCCGCCGCCCAAAACTGGACGGCTGGTGGCGGCCGGGGAATGAGATCAACAGTGCAAGATGAATACTTCAAGTGGATAAGACATATGGGTTCTTTCAAGCACTCTCTTTTCCAAAACACTAAGAACAAGTTCAAGCCTTGTTTGACGTTGAAGGTTTCCAAGAACACCAAGGCTGGTGGTTTCCGCTCGCTTCAGAAGGCGGTGAACTCTTTGCCGATTATCAACCGGTGTCGGGTCCGTATCCATGTTGCTGCTGGAATCTACAGAGAGAAAGTTGAGATTCCAGCGACGATGAGTTACATATGGGTGGAAGGTGAAGGAGCGGAGAAGACAATCATTGAATGGGGCGACACGGCGGACCATATGGGAGAAAATGGCCGTCCTATGGGCACTTTTGCTTCTGCAACTTTTGCTGTTAATTCTCCATTCTTCATTGCTACTAACATCACCTTCAAGAACAAGGCGAGGCTTCCACCATCAGGAGCACTGGGAAAGCAAGCAGTTGCATTCAGAATATCAGGAGATGCAGCAGCATTTATAAGTTGTAGGTTTATCGGTGCACAAGACACATTATACGACCATATGGGGCGACACTACTTCAAGGATTGCTACATTGAAGGGTCAGTGGACTTCGTGTTCGGTGATGGCCTCTCCATCTATGATAGTTGCCATTTGCATGCCATCACCAACAGTTATGGTGCTCTCACGGCCCAAAAGAGGAATAGCATGCTCGAAGAAACTGGCTTCTCTTTCCTTCATTGTAAAGTTTCTGGCTCAGGCGCCTTGTATTTGGGTAGGGCATGGGGCAGCTTCTCTAGAGTTGTGTTTGCTTATACATTTATGGATAAAATCATCACTCCCACTGGTTGGTACAACTGGGGCGACAAAAATCGTGAATTGACTGTATTTTATGGACAATATCGATGCTCGGGACCGGGAGCTGATTATGGAGGAAGGGTTCCATGGTCTAGAGAACTCACTCAGTCAGAAGCCAACCCATTTCTTTCTCTTGATTTTATCAATGCAAACCAATGGCTTCCAAATTACCACACTCATCTCTCTCTTAAACCACATTGA
BLAST of CSPI04G05200 vs. Swiss-Prot
Match: PME53_ARATH (Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 8.3e-130
Identity = 219/371 (59.03%), Postives = 274/371 (73.85%), Query Frame = 1

Query: 8   NLFFLLIAIILPAAAQNWTAGGGR-------GMRSTV------QDEYFKWIRHMGSFKHS 67
           NLF LL+ I+L +        G R        M +T       +DE+ KW+R +GS KHS
Sbjct: 11  NLFLLLLVILLCSTQTQCHTKGLRLRPRNQKNMNTTSDRTQNPEDEFMKWVRFVGSLKHS 70

Query: 68  LFQNTKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIP 127
           +F+  KNK  P  TL V K +  G F  +Q A++SLP+IN  RV I V AG+Y+EKV IP
Sbjct: 71  VFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIP 130

Query: 128 ATMSYIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKA 187
              ++I +EGEGAEKT +EWGDTA      G PMGT+ SA+FAVNSPFF+A NITF+N  
Sbjct: 131 PLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTT 190

Query: 188 RLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGD 247
            +P  GA+GKQAVA R+S D AAF  CR +GAQDTLYDH+GRHY+KDCYIEGSVDF+FG+
Sbjct: 191 PVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGN 250

Query: 248 GLSIYDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVV 307
            LS+Y+ CH+HAI +  GA+TAQ R+S+LE+TGFSF+ CKV+G+G LYLGRAWG FSRVV
Sbjct: 251 ALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVV 310

Query: 308 FAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFL 366
           FAYT+MD II P GWYNWGD +RE+TVFYGQY+C+G GA+YGGRV W+RELT  EA PFL
Sbjct: 311 FAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFL 370

BLAST of CSPI04G05200 vs. Swiss-Prot
Match: PME68_ARATH (Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.7e-79
Identity = 139/287 (48.43%), Postives = 188/287 (65.51%), Query Frame = 1

Query: 80  FRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 139
           FRS+Q AV+S+P  N   + I +A G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 140 HMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFI 199
            +G NG+ + T+ +A+  V + +F A NI+F N A  P  G  G QAVAFRISGD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 200 SCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNSYGALTAQKR 259
            C F GAQDTL D  GRHYFK+CYIEGS+DF+FG+G S+Y  C LH+I + +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 260 NSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDK-NRE 319
               E+TGF+F+ C+V+G+G LY+GRA G +SR+V+AYT+ D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 320 LTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 366
            T F+G Y C GPGA     V W+R L    A+PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of CSPI04G05200 vs. Swiss-Prot
Match: PME62_ARATH (Pectinesterase QRT1 OS=Arabidopsis thaliana GN=QRT1 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.4e-76
Identity = 139/297 (46.80%), Postives = 193/297 (64.98%), Query Frame = 1

Query: 71  VSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEG--AE 130
           V KN   G   ++Q AV+ +P  N  RV+I +  GIYREKV +P +  YI   G    A 
Sbjct: 84  VDKNG-GGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAG 143

Query: 131 KTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVA 190
            T+I W D A  +G +G+ +GT+ +A+ ++ S FF AT ITF+N   +  +G  G+QAVA
Sbjct: 144 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVA 203

Query: 191 FRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAIT 250
            RI GD A F   R +G+QDTL+D  G HYF  CYI+G+VDF+FG+  S+Y  C +H+  
Sbjct: 204 LRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTA 263

Query: 251 NSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTG 310
             YGA+ A  R+S  E+TGFSF++C +SG+G +YLGRAWG++SR V++  F+  IITP G
Sbjct: 264 KRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVG 323

Query: 311 WYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 366
           W +W    R+  V +G+Y C G GA+ GGRVPWS+ LT+ E  PFL  +FI  +QWL
Sbjct: 324 WSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

BLAST of CSPI04G05200 vs. Swiss-Prot
Match: PME15_ARATH (Probable pectinesterase 15 OS=Arabidopsis thaliana GN=PME15 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.6e-75
Identity = 141/318 (44.34%), Postives = 204/318 (64.15%), Query Frame = 1

Query: 60  KNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSY 119
           ++++K  L L V  +   G F ++Q A++ +P ++  +  I V +G YREKV +    + 
Sbjct: 82  RHQYKTSLVLTVDLHG-CGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTN 141

Query: 120 IWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPS 179
           + ++G G + T IEW DTA   G       +F+   FA N   F A NI+FKN A  P  
Sbjct: 142 LVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEPDP 201

Query: 180 GALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 239
           G    QAVA RI GD AAF  C F GAQDTL D  GRH+FK+C+I+GS+DF+FG+G S+Y
Sbjct: 202 GEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLY 261

Query: 240 DSCHLHAITNSY-----GALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVV 299
             C +++I         G++TAQ R S  E++GFSF++CK+ GSG + LGRAWG+++ VV
Sbjct: 262 QDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVV 321

Query: 300 FAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFL 359
           F+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++LT SEA+ F+
Sbjct: 322 FSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFI 381

Query: 360 SLDFINANQWLPNYHTHL 373
            + FI+ ++WL   HT++
Sbjct: 382 DVSFIDGDEWL--RHTNI 390

BLAST of CSPI04G05200 vs. Swiss-Prot
Match: PME8_ARATH (Probable pectinesterase 8 OS=Arabidopsis thaliana GN=PME8 PE=2 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 3.3e-70
Identity = 131/294 (44.56%), Postives = 187/294 (63.61%), Query Frame = 1

Query: 80  FRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 139
           F ++Q AV+++   ++ R  I + +G+Y EKV IP T   I ++G+G + T I W DTA 
Sbjct: 101 FTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAY 160

Query: 140 HMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFI 199
                    GTF  AT  V    F+A NI+F N A +P  G +G QAVA RI+GD +AF+
Sbjct: 161 SAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 220

Query: 200 SCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNSY-------- 259
            C F GAQDTL+D  GRHYFKDCYI+GS+DF+FG+  S+Y  C + ++ N          
Sbjct: 221 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 280

Query: 260 GALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYN 319
           GA+TA  R+S  E +GFSF++C + G+G ++LGRAW  +SRVVF  T M  +I P GW N
Sbjct: 281 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 340

Query: 320 WGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 366
           + D +R+ T+FYG+Y CSGPGAD   R P+ ++L +++    ++  FI+ +QWL
Sbjct: 341 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWL 388

BLAST of CSPI04G05200 vs. TrEMBL
Match: A0A0A0KWM1_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_4G043950 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 4.7e-225
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 1

Query: 1   MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK 60
           MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK
Sbjct: 1   MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK 60

Query: 61  NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI 120
           NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI
Sbjct: 61  NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI 120

Query: 121 WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG 180
           WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG
Sbjct: 121 WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG 180

Query: 181 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 240
           ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD
Sbjct: 181 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 240

Query: 241 SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM 300
           SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM
Sbjct: 241 SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM 300

Query: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN 360
           DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN
Sbjct: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN 360

Query: 361 ANQWLPNYHTHLSLKPH 378
           ANQWLPNYHTHLSLKPH
Sbjct: 361 ANQWLPNYHTHLSLKPH 377

BLAST of CSPI04G05200 vs. TrEMBL
Match: B9SZT7_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_0291660 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.1e-152
Identity = 256/361 (70.91%), Postives = 303/361 (83.93%), Query Frame = 1

Query: 9   LFFLLIAIILPAAAQNW-TAGGGRGMRSTVQDE-YFKWIR--HMGSFKHSLFQNTKNKFK 68
           LF LL  I+L  +A +W T+ G    R  V +E Y +W++   MGSFKH+LFQ  KNKFK
Sbjct: 4   LFKLLCLILLSGSATSWFTSNGSNSNRFVVNEEAYLQWLKAKQMGSFKHALFQKAKNKFK 63

Query: 69  PCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEG 128
           PCLT+KV+K +K+G F +L+KA+NS+P+IN CRV I + AG YREK+EIP +MSYI +EG
Sbjct: 64  PCLTIKVNKKSKSGDFVTLKKALNSIPVINNCRVIISIGAGTYREKIEIPGSMSYITLEG 123

Query: 129 EGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGK 188
            GA KT IEW DTAD  G+ G  +GT+ SATFA+NSP+FIA NITFKNKA  PPSGALGK
Sbjct: 124 AGAGKTTIEWDDTADKQGQGGHLLGTYGSATFAINSPYFIAKNITFKNKAPSPPSGALGK 183

Query: 189 QAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHL 248
           QAVA RIS D AAFI C+FIGAQDTLYDH+GRHYFK+CYIEGSVDF+FG+GLS+YD CHL
Sbjct: 184 QAVALRISADTAAFIGCKFIGAQDTLYDHIGRHYFKECYIEGSVDFIFGNGLSLYDDCHL 243

Query: 249 HAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKII 308
           HAITNS+GALTAQKR SMLEETGFSF++CKV+GSGALYLGRAWG+FSRVVFAYT+MDKII
Sbjct: 244 HAITNSFGALTAQKRESMLEETGFSFVNCKVTGSGALYLGRAWGTFSRVVFAYTYMDKII 303

Query: 309 TPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQW 366
           TPTGWY+WGDKNRE+TVFYGQY+CSGPGA +GGRV WSRELT+ EA PF+S+DFI+   W
Sbjct: 304 TPTGWYDWGDKNREMTVFYGQYKCSGPGAQFGGRVSWSRELTEQEAKPFVSIDFIDGQDW 363

BLAST of CSPI04G05200 vs. TrEMBL
Match: U5G8L2_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0007s13780g PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.1e-152
Identity = 247/330 (74.85%), Postives = 294/330 (89.09%), Query Frame = 1

Query: 38  QDEYFKWIRHMGSFKHSLFQNTKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCR 97
           +++YFKW++HMGSFKHSLFQ  KNKFKPCLT++VSK  ++G F ++QKA+NSLP+IN CR
Sbjct: 12  EEDYFKWLKHMGSFKHSLFQKAKNKFKPCLTIEVSKKPRSGAFPTVQKAINSLPVINNCR 71

Query: 98  VRIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFA 157
           V I ++AG YREKVEIPATM+YI + G GA++TIIEW DTAD M ENGRP+GTF SATFA
Sbjct: 72  VVISISAGTYREKVEIPATMAYITLRGAGADRTIIEWDDTADRM-ENGRPLGTFGSATFA 131

Query: 158 VNSPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRH 217
           VNSP+FIA +ITFKNKA LPPSGALGKQAVA RIS D AAFISC+FIGAQDTLYDH+GRH
Sbjct: 132 VNSPYFIAKDITFKNKAPLPPSGALGKQAVALRISADTAAFISCKFIGAQDTLYDHIGRH 191

Query: 218 YFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSG 277
           YFK CYIEGSVDF+FG+GLS+Y+ CHLHA+T S+GALTAQKR S LEETGFSF+ CKV+G
Sbjct: 192 YFKKCYIEGSVDFIFGNGLSLYEDCHLHAVTTSFGALTAQKRQSFLEETGFSFVSCKVTG 251

Query: 278 SGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGG 337
           SGAL+LGRAWG+FSRVVFAYTFMDKIITP GWY+WGDK+R++TVF+GQY+CSGPGAD+GG
Sbjct: 252 SGALFLGRAWGNFSRVVFAYTFMDKIITPRGWYDWGDKSRQMTVFFGQYKCSGPGADFGG 311

Query: 338 RVPWSRELTQSEANPFLSLDFINANQWLPN 368
           RV WSRELT  +A PF+S+ FI+ ++WL N
Sbjct: 312 RVAWSRELTDQQAKPFISIGFIDGHEWLLN 340

BLAST of CSPI04G05200 vs. TrEMBL
Match: B9HFI2_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0007s13780g PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.2e-151
Identity = 247/328 (75.30%), Postives = 292/328 (89.02%), Query Frame = 1

Query: 40  EYFKWIRHMGSFKHSLFQNTKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVR 99
           +YFKW++HMGSFKHSLFQ  KNKFKPCLT++VSK  ++G F ++QKA+NSLP+IN CRV 
Sbjct: 12  DYFKWLKHMGSFKHSLFQKAKNKFKPCLTIEVSKKPRSGAFPTVQKAINSLPVINNCRVV 71

Query: 100 IHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVN 159
           I ++AG YREKVEIPATM+YI + G GA++TIIEW DTAD M ENGRP+GTF SATFAVN
Sbjct: 72  ISISAGTYREKVEIPATMAYITLRGAGADRTIIEWDDTADRM-ENGRPLGTFGSATFAVN 131

Query: 160 SPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYF 219
           SP+FIA +ITFKNKA LPPSGALGKQAVA RIS D AAFISC+FIGAQDTLYDH+GRHYF
Sbjct: 132 SPYFIAKDITFKNKAPLPPSGALGKQAVALRISADTAAFISCKFIGAQDTLYDHIGRHYF 191

Query: 220 KDCYIEGSVDFVFGDGLSIYDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSG 279
           K CYIEGSVDF+FG+GLS+Y+ CHLHA+T S+GALTAQKR S LEETGFSF+ CKV+GSG
Sbjct: 192 KKCYIEGSVDFIFGNGLSLYEDCHLHAVTTSFGALTAQKRQSFLEETGFSFVSCKVTGSG 251

Query: 280 ALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV 339
           AL+LGRAWG+FSRVVFAYTFMDKIITP GWY+WGDK+R++TVF+GQY+CSGPGAD+GGRV
Sbjct: 252 ALFLGRAWGNFSRVVFAYTFMDKIITPRGWYDWGDKSRQMTVFFGQYKCSGPGADFGGRV 311

Query: 340 PWSRELTQSEANPFLSLDFINANQWLPN 368
            WSRELT  +A PF+S+ FI+ ++WL N
Sbjct: 312 AWSRELTDQQAKPFISIGFIDGHEWLLN 338

BLAST of CSPI04G05200 vs. TrEMBL
Match: V4SNY8_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10027321mg PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 5.9e-151
Identity = 252/363 (69.42%), Postives = 300/363 (82.64%), Query Frame = 1

Query: 9   LFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWI---RHMGSFKHSLFQNTKNKFKP 68
           L   L+A++L   + +W A       + V+  Y  W+   +H     HSLFQ  KNKFKP
Sbjct: 3   LKLFLLALLLERTS-SWVASNESNDLAVVEQGYLNWVNFKQHAPHLNHSLFQKAKNKFKP 62

Query: 69  CLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGE 128
           C T+KV+KN + G F ++QKA+NSLP+IN CRV I ++AG YREKVEIP TM+YI + G 
Sbjct: 63  CKTIKVNKNPRLGNFVTVQKAINSLPVINLCRVVIFISAGTYREKVEIPTTMAYITMIGA 122

Query: 129 GAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQ 188
           GA+ T+IEW DTAD MG +GRP+GT+ASATFAVNSP+FIA NITFKNKA LPPSGALGKQ
Sbjct: 123 GADNTVIEWDDTADRMGRSGRPLGTYASATFAVNSPYFIAKNITFKNKAPLPPSGALGKQ 182

Query: 189 AVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLH 248
           AVAFRIS D AAF  C+FIG+QDTLYDH+GRHYFK+CYIEGSVDF+FG+GLS Y+ CHLH
Sbjct: 183 AVAFRISADTAAFTGCKFIGSQDTLYDHIGRHYFKNCYIEGSVDFIFGNGLSFYEDCHLH 242

Query: 249 AITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIIT 308
           AITNSYGALTAQKR S+LEETGFSF+ CKV+GSGALYLGRAWG+FSRVVFAYT+MDKIIT
Sbjct: 243 AITNSYGALTAQKRGSLLEETGFSFVKCKVTGSGALYLGRAWGTFSRVVFAYTYMDKIIT 302

Query: 309 PTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 368
           P GWY+WGDKNRE+TVFYGQY+CSGPGA YGGRV WSRELTQ EA PF+S++FI+ +QWL
Sbjct: 303 PRGWYDWGDKNREMTVFYGQYKCSGPGAYYGGRVSWSRELTQEEAEPFISVEFIDGHQWL 362

BLAST of CSPI04G05200 vs. TAIR10
Match: AT5G19730.1 (AT5G19730.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 464.9 bits (1195), Expect = 4.7e-131
Identity = 219/371 (59.03%), Postives = 274/371 (73.85%), Query Frame = 1

Query: 8   NLFFLLIAIILPAAAQNWTAGGGR-------GMRSTV------QDEYFKWIRHMGSFKHS 67
           NLF LL+ I+L +        G R        M +T       +DE+ KW+R +GS KHS
Sbjct: 11  NLFLLLLVILLCSTQTQCHTKGLRLRPRNQKNMNTTSDRTQNPEDEFMKWVRFVGSLKHS 70

Query: 68  LFQNTKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIP 127
           +F+  KNK  P  TL V K +  G F  +Q A++SLP+IN  RV I V AG+Y+EKV IP
Sbjct: 71  VFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIP 130

Query: 128 ATMSYIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKA 187
              ++I +EGEGAEKT +EWGDTA      G PMGT+ SA+FAVNSPFF+A NITF+N  
Sbjct: 131 PLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTT 190

Query: 188 RLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGD 247
            +P  GA+GKQAVA R+S D AAF  CR +GAQDTLYDH+GRHY+KDCYIEGSVDF+FG+
Sbjct: 191 PVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGN 250

Query: 248 GLSIYDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVV 307
            LS+Y+ CH+HAI +  GA+TAQ R+S+LE+TGFSF+ CKV+G+G LYLGRAWG FSRVV
Sbjct: 251 ALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVV 310

Query: 308 FAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFL 366
           FAYT+MD II P GWYNWGD +RE+TVFYGQY+C+G GA+YGGRV W+RELT  EA PFL
Sbjct: 311 FAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFL 370

BLAST of CSPI04G05200 vs. TAIR10
Match: AT5G47500.1 (AT5G47500.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 295.4 bits (755), Expect = 4.9e-80
Identity = 139/287 (48.43%), Postives = 188/287 (65.51%), Query Frame = 1

Query: 80  FRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 139
           FRS+Q AV+S+P  N   + I +A G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 140 HMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFI 199
            +G NG+ + T+ +A+  V + +F A NI+F N A  P  G  G QAVAFRISGD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 200 SCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNSYGALTAQKR 259
            C F GAQDTL D  GRHYFK+CYIEGS+DF+FG+G S+Y  C LH+I + +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 260 NSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDK-NRE 319
               E+TGF+F+ C+V+G+G LY+GRA G +SR+V+AYT+ D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 320 LTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 366
            T F+G Y C GPGA     V W+R L    A+PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of CSPI04G05200 vs. TAIR10
Match: AT5G55590.1 (AT5G55590.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 288.1 bits (736), Expect = 7.8e-78
Identity = 139/297 (46.80%), Postives = 193/297 (64.98%), Query Frame = 1

Query: 71  VSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEG--AE 130
           V KN   G   ++Q AV+ +P  N  RV+I +  GIYREKV +P +  YI   G    A 
Sbjct: 84  VDKNG-GGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAG 143

Query: 131 KTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVA 190
            T+I W D A  +G +G+ +GT+ +A+ ++ S FF AT ITF+N   +  +G  G+QAVA
Sbjct: 144 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVA 203

Query: 191 FRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAIT 250
            RI GD A F   R +G+QDTL+D  G HYF  CYI+G+VDF+FG+  S+Y  C +H+  
Sbjct: 204 LRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTA 263

Query: 251 NSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTG 310
             YGA+ A  R+S  E+TGFSF++C +SG+G +YLGRAWG++SR V++  F+  IITP G
Sbjct: 264 KRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVG 323

Query: 311 WYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 366
           W +W    R+  V +G+Y C G GA+ GGRVPWS+ LT+ E  PFL  +FI  +QWL
Sbjct: 324 WSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

BLAST of CSPI04G05200 vs. TAIR10
Match: AT2G36710.1 (AT2G36710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 282.3 bits (721), Expect = 4.3e-76
Identity = 141/318 (44.34%), Postives = 204/318 (64.15%), Query Frame = 1

Query: 60  KNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSY 119
           ++++K  L L V  +   G F ++Q A++ +P ++  +  I V +G YREKV +    + 
Sbjct: 82  RHQYKTSLVLTVDLHG-CGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTN 141

Query: 120 IWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPS 179
           + ++G G + T IEW DTA   G       +F+   FA N   F A NI+FKN A  P  
Sbjct: 142 LVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEPDP 201

Query: 180 GALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIY 239
           G    QAVA RI GD AAF  C F GAQDTL D  GRH+FK+C+I+GS+DF+FG+G S+Y
Sbjct: 202 GEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLY 261

Query: 240 DSCHLHAITNSY-----GALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVV 299
             C +++I         G++TAQ R S  E++GFSF++CK+ GSG + LGRAWG+++ VV
Sbjct: 262 QDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVV 321

Query: 300 FAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFL 359
           F+ T+M  IITP GW NWGD  +E TV +G+++C GPGADY  RV + ++LT SEA+ F+
Sbjct: 322 FSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFI 381

Query: 360 SLDFINANQWLPNYHTHL 373
            + FI+ ++WL   HT++
Sbjct: 382 DVSFIDGDEWL--RHTNI 390

BLAST of CSPI04G05200 vs. TAIR10
Match: AT1G05310.1 (AT1G05310.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 266.9 bits (681), Expect = 1.9e-71
Identity = 131/294 (44.56%), Postives = 187/294 (63.61%), Query Frame = 1

Query: 80  FRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTAD 139
           F ++Q AV+++   ++ R  I + +G+Y EKV IP T   I ++G+G + T I W DTA 
Sbjct: 101 FTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAY 160

Query: 140 HMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFI 199
                    GTF  AT  V    F+A NI+F N A +P  G +G QAVA RI+GD +AF+
Sbjct: 161 SAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 220

Query: 200 SCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNSY-------- 259
            C F GAQDTL+D  GRHYFKDCYI+GS+DF+FG+  S+Y  C + ++ N          
Sbjct: 221 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 280

Query: 260 GALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYN 319
           GA+TA  R+S  E +GFSF++C + G+G ++LGRAW  +SRVVF  T M  +I P GW N
Sbjct: 281 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 340

Query: 320 WGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQWL 366
           + D +R+ T+FYG+Y CSGPGAD   R P+ ++L +++    ++  FI+ +QWL
Sbjct: 341 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWL 388

BLAST of CSPI04G05200 vs. NCBI nr
Match: gi|449457504|ref|XP_004146488.1| (PREDICTED: probable pectinesterase 53 [Cucumis sativus])

HSP 1 Score: 788.1 bits (2034), Expect = 6.8e-225
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 1

Query: 1   MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK 60
           MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK
Sbjct: 1   MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK 60

Query: 61  NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI 120
           NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI
Sbjct: 61  NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI 120

Query: 121 WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG 180
           WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG
Sbjct: 121 WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG 180

Query: 181 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 240
           ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD
Sbjct: 181 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 240

Query: 241 SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM 300
           SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM
Sbjct: 241 SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM 300

Query: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN 360
           DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN
Sbjct: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN 360

Query: 361 ANQWLPNYHTHLSLKPH 378
           ANQWLPNYHTHLSLKPH
Sbjct: 361 ANQWLPNYHTHLSLKPH 377

BLAST of CSPI04G05200 vs. NCBI nr
Match: gi|659130584|ref|XP_008465244.1| (PREDICTED: probable pectinesterase 53 [Cucumis melo])

HSP 1 Score: 761.5 bits (1965), Expect = 6.8e-217
Identity = 367/375 (97.87%), Postives = 367/375 (97.87%), Query Frame = 1

Query: 3   RPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTKNK 62
           RPLIFNL FLLIAIILPAAAQNWTAGGGRGMR TVQDEY KWIRHMGSFKHSLFQNTKNK
Sbjct: 2   RPLIFNLLFLLIAIILPAAAQNWTAGGGRGMR-TVQDEYLKWIRHMGSFKHSLFQNTKNK 61

Query: 63  FKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWV 122
           FKPCLTLKVSKN K GGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWV
Sbjct: 62  FKPCLTLKVSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWV 121

Query: 123 EGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGAL 182
           EGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGAL
Sbjct: 122 EGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGAL 181

Query: 183 GKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSC 242
           GKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSC
Sbjct: 182 GKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSC 241

Query: 243 HLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDK 302
           HLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDK
Sbjct: 242 HLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDK 301

Query: 303 IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINAN 362
           IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRV WSRELTQSEANPFLSLDFINA 
Sbjct: 302 IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAK 361

Query: 363 QWLPNYHTHLSLKPH 378
           QWLPNYHTHLS KPH
Sbjct: 362 QWLPNYHTHLSHKPH 375

BLAST of CSPI04G05200 vs. NCBI nr
Match: gi|720049550|ref|XP_010271483.1| (PREDICTED: probable pectinesterase 53 [Nelumbo nucifera])

HSP 1 Score: 548.9 bits (1413), Expect = 6.9e-153
Identity = 253/363 (69.70%), Postives = 305/363 (84.02%), Query Frame = 1

Query: 4   PLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTKNKF 63
           P++ +LF L++  +   A  + +  G  G    +Q+ Y  WI+ MGS  HSL+   +NK 
Sbjct: 36  PMLSSLFLLIL--LFEGAPSSVSLNGTNGT-VIIQEGYLNWIKQMGSLTHSLYNKAENKL 95

Query: 64  KPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVE 123
           KPCLT+ V K+ K G F ++QKAV+SLPI+N+CRV I + AGIYREKV+IPAT +++ +E
Sbjct: 96  KPCLTITVDKDPKKGDFVTVQKAVDSLPIVNQCRVLIKIHAGIYREKVDIPATKAFVTLE 155

Query: 124 GEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALG 183
           G GA++TI++WGDTAD MG + RP+GTFASATFA+NSP+F+A NITFKN A LPPSGALG
Sbjct: 156 GAGADRTILQWGDTADRMGSDRRPLGTFASATFAINSPYFVAKNITFKNTAPLPPSGALG 215

Query: 184 KQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCH 243
           KQAVA RISGD AAF SC FIGAQDTLYDH+GRHYFKDCYI+GSVDF+FGDGLS+Y+ CH
Sbjct: 216 KQAVALRISGDTAAFFSCNFIGAQDTLYDHIGRHYFKDCYIQGSVDFIFGDGLSLYEGCH 275

Query: 244 LHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKI 303
           LHAITNSYGALTAQKR++MLEETGFSF++C+V+GSGALYLGRAWGSFSRVVFAYT+MDKI
Sbjct: 276 LHAITNSYGALTAQKRDNMLEETGFSFVNCRVTGSGALYLGRAWGSFSRVVFAYTYMDKI 335

Query: 304 ITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQ 363
           ITP GWYNWGDKNRELTVFYGQY+CSGPGAD+GGRV WSRELTQ EA PF+SL FI+  +
Sbjct: 336 ITPKGWYNWGDKNRELTVFYGQYKCSGPGADFGGRVSWSRELTQQEAKPFISLSFIDGYE 395

Query: 364 WLP 367
           WLP
Sbjct: 396 WLP 395

BLAST of CSPI04G05200 vs. NCBI nr
Match: gi|255581396|ref|XP_002531506.1| (PREDICTED: probable pectinesterase 53 [Ricinus communis])

HSP 1 Score: 547.7 bits (1410), Expect = 1.5e-152
Identity = 256/361 (70.91%), Postives = 303/361 (83.93%), Query Frame = 1

Query: 9   LFFLLIAIILPAAAQNW-TAGGGRGMRSTVQDE-YFKWIR--HMGSFKHSLFQNTKNKFK 68
           LF LL  I+L  +A +W T+ G    R  V +E Y +W++   MGSFKH+LFQ  KNKFK
Sbjct: 4   LFKLLCLILLSGSATSWFTSNGSNSNRFVVNEEAYLQWLKAKQMGSFKHALFQKAKNKFK 63

Query: 69  PCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEG 128
           PCLT+KV+K +K+G F +L+KA+NS+P+IN CRV I + AG YREK+EIP +MSYI +EG
Sbjct: 64  PCLTIKVNKKSKSGDFVTLKKALNSIPVINNCRVIISIGAGTYREKIEIPGSMSYITLEG 123

Query: 129 EGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGK 188
            GA KT IEW DTAD  G+ G  +GT+ SATFA+NSP+FIA NITFKNKA  PPSGALGK
Sbjct: 124 AGAGKTTIEWDDTADKQGQGGHLLGTYGSATFAINSPYFIAKNITFKNKAPSPPSGALGK 183

Query: 189 QAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHL 248
           QAVA RIS D AAFI C+FIGAQDTLYDH+GRHYFK+CYIEGSVDF+FG+GLS+YD CHL
Sbjct: 184 QAVALRISADTAAFIGCKFIGAQDTLYDHIGRHYFKECYIEGSVDFIFGNGLSLYDDCHL 243

Query: 249 HAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKII 308
           HAITNS+GALTAQKR SMLEETGFSF++CKV+GSGALYLGRAWG+FSRVVFAYT+MDKII
Sbjct: 244 HAITNSFGALTAQKRESMLEETGFSFVNCKVTGSGALYLGRAWGTFSRVVFAYTYMDKII 303

Query: 309 TPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFINANQW 366
           TPTGWY+WGDKNRE+TVFYGQY+CSGPGA +GGRV WSRELT+ EA PF+S+DFI+   W
Sbjct: 304 TPTGWYDWGDKNREMTVFYGQYKCSGPGAQFGGRVSWSRELTEQEAKPFVSIDFIDGQDW 363

BLAST of CSPI04G05200 vs. NCBI nr
Match: gi|566181194|ref|XP_006380792.1| (hypothetical protein POPTR_0007s13780g [Populus trichocarpa])

HSP 1 Score: 545.8 bits (1405), Expect = 5.9e-152
Identity = 247/330 (74.85%), Postives = 294/330 (89.09%), Query Frame = 1

Query: 38  QDEYFKWIRHMGSFKHSLFQNTKNKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCR 97
           +++YFKW++HMGSFKHSLFQ  KNKFKPCLT++VSK  ++G F ++QKA+NSLP+IN CR
Sbjct: 12  EEDYFKWLKHMGSFKHSLFQKAKNKFKPCLTIEVSKKPRSGAFPTVQKAINSLPVINNCR 71

Query: 98  VRIHVAAGIYREKVEIPATMSYIWVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFA 157
           V I ++AG YREKVEIPATM+YI + G GA++TIIEW DTAD M ENGRP+GTF SATFA
Sbjct: 72  VVISISAGTYREKVEIPATMAYITLRGAGADRTIIEWDDTADRM-ENGRPLGTFGSATFA 131

Query: 158 VNSPFFIATNITFKNKARLPPSGALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRH 217
           VNSP+FIA +ITFKNKA LPPSGALGKQAVA RIS D AAFISC+FIGAQDTLYDH+GRH
Sbjct: 132 VNSPYFIAKDITFKNKAPLPPSGALGKQAVALRISADTAAFISCKFIGAQDTLYDHIGRH 191

Query: 218 YFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSG 277
           YFK CYIEGSVDF+FG+GLS+Y+ CHLHA+T S+GALTAQKR S LEETGFSF+ CKV+G
Sbjct: 192 YFKKCYIEGSVDFIFGNGLSLYEDCHLHAVTTSFGALTAQKRQSFLEETGFSFVSCKVTG 251

Query: 278 SGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGG 337
           SGAL+LGRAWG+FSRVVFAYTFMDKIITP GWY+WGDK+R++TVF+GQY+CSGPGAD+GG
Sbjct: 252 SGALFLGRAWGNFSRVVFAYTFMDKIITPRGWYDWGDKSRQMTVFFGQYKCSGPGADFGG 311

Query: 338 RVPWSRELTQSEANPFLSLDFINANQWLPN 368
           RV WSRELT  +A PF+S+ FI+ ++WL N
Sbjct: 312 RVAWSRELTDQQAKPFISIGFIDGHEWLLN 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME53_ARATH8.3e-13059.03Probable pectinesterase 53 OS=Arabidopsis thaliana GN=PME53 PE=2 SV=1[more]
PME68_ARATH8.7e-7948.43Probable pectinesterase 68 OS=Arabidopsis thaliana GN=PME68 PE=2 SV=1[more]
PME62_ARATH1.4e-7646.80Pectinesterase QRT1 OS=Arabidopsis thaliana GN=QRT1 PE=2 SV=1[more]
PME15_ARATH7.6e-7544.34Probable pectinesterase 15 OS=Arabidopsis thaliana GN=PME15 PE=2 SV=1[more]
PME8_ARATH3.3e-7044.56Probable pectinesterase 8 OS=Arabidopsis thaliana GN=PME8 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KWM1_CUCSA4.7e-225100.00Pectinesterase OS=Cucumis sativus GN=Csa_4G043950 PE=4 SV=1[more]
B9SZT7_RICCO1.1e-15270.91Pectinesterase OS=Ricinus communis GN=RCOM_0291660 PE=4 SV=1[more]
U5G8L2_POPTR4.1e-15274.85Pectinesterase OS=Populus trichocarpa GN=POPTR_0007s13780g PE=4 SV=1[more]
B9HFI2_POPTR1.2e-15175.30Pectinesterase OS=Populus trichocarpa GN=POPTR_0007s13780g PE=4 SV=1[more]
V4SNY8_9ROSI5.9e-15169.42Pectinesterase OS=Citrus clementina GN=CICLE_v10027321mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G19730.14.7e-13159.03 Pectin lyase-like superfamily protein[more]
AT5G47500.14.9e-8048.43 Pectin lyase-like superfamily protein[more]
AT5G55590.17.8e-7846.80 Pectin lyase-like superfamily protein[more]
AT2G36710.14.3e-7644.34 Pectin lyase-like superfamily protein[more]
AT1G05310.11.9e-7144.56 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449457504|ref|XP_004146488.1|6.8e-225100.00PREDICTED: probable pectinesterase 53 [Cucumis sativus][more]
gi|659130584|ref|XP_008465244.1|6.8e-21797.87PREDICTED: probable pectinesterase 53 [Cucumis melo][more]
gi|720049550|ref|XP_010271483.1|6.9e-15369.70PREDICTED: probable pectinesterase 53 [Nelumbo nucifera][more]
gi|255581396|ref|XP_002531506.1|1.5e-15270.91PREDICTED: probable pectinesterase 53 [Ricinus communis][more]
gi|566181194|ref|XP_006380792.1|5.9e-15274.85hypothetical protein POPTR_0007s13780g [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G05200.1CSPI04G05200.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 76..361
score: 1.2
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 68..373
score: 2.51
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 68..369
score: 2.9E
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 39..365
score: 2.5E
NoneNo IPR availablePANTHERPTHR31321:SF25SUBFAMILY NOT NAMEDcoord: 39..365
score: 2.5E

The following gene(s) are paralogous to this gene:

None