Lsi06G010910 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi06G010910
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Locationchr06 : 21199943 .. 21202415 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTAACAATCTCAATTAAAACCCAATTTTCCACCATAATAATGATTATTAAGATTGCACATTCCTCTTGGGGGCGTACCAAACTTCTCAGCAATCGACAAATTGCATTTCTCTCACTTTCCCCATTTTTCACCATCATTTCATGCCATATAGTTCACCAAACATCTGACTTACCATACTCCCTCTTCTCCATTTCTATAAAATCATCCCCATTCCACACAAACACAGTGCTCACACGAAACAGAGACACCCAAAAATGGATCATACAAAGCCAATTCTTTGCGTTCTTGACGCTTCCTCTTACGTCGGCTTTTGGGTCACCAAAGAATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAGAACACTGGCAAGTCATCTATCATCCTCTGTTCTTTTCCATTTCTTAATTCTCTGTTTCTATTTCAAATTTGTGTGTTTCATATATTCCAGGAAAGCCAGAGATTGAGAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGTCTGGTTGTTTTTCCTGTAGATATCATGGATTACCAGAGCATTCTTGTTGCTTTGAAAGGATGCTCTGGTTTGTTCTGCTGTATGGACACCCCCCATGTGTTTGATGTAAGTCCACAGAGAAAAAATAAATAAATAAATGAATAAAAAGTTGATCTGGAATTTGTGGGTTATTGTTGCTTTTGTGTTTTATGCAGGAAAAAATGGTGGATTTGGAAGTTAGAGGGACAATTAATGTTGTAGAGGCTTGTGCTCAGACAGATACTGTTGATAAGATTGTCTTCACTTCCTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTATCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGGTTGGGAATTTCAAATCCCTCTTCTCATCTTCATAAAGTTCTTCAAATGGCAGATACCTAATCAATCAATTTCATTGCAAATCAAGCTTTCTCTTTACAAATCTAATGACCCTTTTTTTTTTTATATTAATATCATCTCCCAGTTGTGGTACCCTCTAGCAAAGACACTCTCTGAACAAGCTGCGTGGGCTCTAGCGATGGACCGTAGGCTCAAAATGGTGTCAATTAACGCAGGGCTTGTTCTTGGACCGGCTGTTGCAGAAGAAAATTCAGGGTCAACAATCTCATATCTTAAAGGTAGGAGAAAGTTTTTAAGAAAAGTATTGTACAATTGTGGGAGATTTTGTTTTAGCTAAGAAAATTTGAGTGGAAACAGGAGCTGCTCAGATGTATGAAAATGGGGTGCTGGCAGTGGTTGATGTGAAGTTTTTGGTGGATGTTCATATCCGAGCAATGGAAGATTCGTCCACGGGTGGTCGATATTTCTGCTTCGATCAGATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTTCCACAAAGGTAAAAGTATTATTAGCCTTTTGAACAACAAACACAACAATTTCATAAAAAAATATAATTTGGTGCATCCCGCACATCACTTATTTTTGTTTATTCTATCAAATTGTCTAATAATATGTGGCGAATTCTTTCGATTTTTTTTTAAATATTTTAATTTTTTTTTGTGTGTGAGTGATGATAGAAGATGCATAAAGATAAGTGCATCCAAGTATTACTCATTTTAGAATAGAAAATTAGGTATTTTTTTAGTCCATTTTGTCCAAAAGTACCTAGGTGTTCCTTGTACAGTCCAAGGAGTTATCCCGTAAACAACTACCGCATGGTATTATTTTTTTTTTTAAAATAATTTTTTTTAATGAAATTAGGTGTAGTCTAGACTACATCTAATTATTCACCTTTTTACCATGCATTTGTATGTTATTGTTAAGACTACTCTTAATTATACATTAAAAAAAAAAACTCAAAAAATATTTTTTTAACAAAAAAACAAAAATTTGTTATGTCATAACCAGATCAACAGGACCAAGTGTGTGAAAAAGAAATAAAAAAATATTCTTTTTAAATAAAATAAAAATTTGTTACGTGGCAAATATTGATCGATAACTGGGCAGTTGTAAAAAGGAAATCAAAATTTTTAATTAAAAGATAATTGGGTGAATTGTAAAAAAATAAATACGGTTAATTGAAACTCAAACTTATGGTTAGTTTATTGGTTGGTGAGCAGTTACGAAGTCCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACCAGGAGATTGCACAAACTGATCGAGGGCACAGCTTAATAATAAACTTTAAATGGAACTATTTTCTTTTTTCTTTTCTTTTTTTTAGTATGTATCTTTTTAGTACTTAAGTAATAAAAAATTACTATTTAGCATGCACGTAAATGGGACATCATAAATTATAAAGCCGAATATAATTCAATTGATAAGATAGTTGTTATCATTTCAACTCAAATTTTCATAAACTATATATATATAAACCATG

mRNA sequence

GATTAACAATCTCAATTAAAACCCAATTTTCCACCATAATAATGATTATTAAGATTGCACATTCCTCTTGGGGGCGTACCAAACTTCTCAGCAATCGACAAATTGCATTTCTCTCACTTTCCCCATTTTTCACCATCATTTCATGCCATATAGTTCACCAAACATCTGACTTACCATACTCCCTCTTCTCCATTTCTATAAAATCATCCCCATTCCACACAAACACAGTGCTCACACGAAACAGAGACACCCAAAAATGGATCATACAAAGCCAATTCTTTGCGTTCTTGACGCTTCCTCTTACGTCGGCTTTTGGGTCACCAAAGAATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAGAACACTGGAAAGCCAGAGATTGAGAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGTCTGGTTGTTTTTCCTGTAGATATCATGGATTACCAGAGCATTCTTGTTGCTTTGAAAGGATGCTCTGGTTTGTTCTGCTGTATGGACACCCCCCATGTGTTTGATGAAAAAATGGTGGATTTGGAAGTTAGAGGGACAATTAATGTTGTAGAGGCTTGTGCTCAGACAGATACTGTTGATAAGATTGTCTTCACTTCCTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTATCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGTTGTGGTACCCTCTAGCAAAGACACTCTCTGAACAAGCTGCGTGGGCTCTAGCGATGGACCGTAGGCTCAAAATGGTGTCAATTAACGCAGGGCTTGTTCTTGGACCGGCTGTTGCAGAAGAAAATTCAGGGTCAACAATCTCATATCTTAAAGGAGCTGCTCAGATGTATGAAAATGGGGTGCTGGCAGTGGTTGATGTGAAGTTTTTGGTGGATGTTCATATCCGAGCAATGGAAGATTCGTCCACGGGTGGTCGATATTTCTGCTTCGATCAGATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTTCCACAAAGTTACGAAGTCCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACCAGGAGATTGCACAAACTGATCGAGGGCACAGCTTAATAATAAACTTTAAATGGAACTATTTTCTTTTTTCTTTTCTTTTTTTTAGTATGTATCTTTTTAGTACTTAAGTAATAAAAAATTACTATTTAGCATGCACGTAAATGGGACATCATAAATTATAAAGCCGAATATAATTCAATTGATAAGATAGTTGTTATCATTTCAACTCAAATTTTCATAAACTATATATATATAAACCATG

Coding sequence (CDS)

ATGGATCATACAAAGCCAATTCTTTGCGTTCTTGACGCTTCCTCTTACGTCGGCTTTTGGGTCACCAAAGAATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAGAACACTGGAAAGCCAGAGATTGAGAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGTCTGGTTGTTTTTCCTGTAGATATCATGGATTACCAGAGCATTCTTGTTGCTTTGAAAGGATGCTCTGGTTTGTTCTGCTGTATGGACACCCCCCATGTGTTTGATGAAAAAATGGTGGATTTGGAAGTTAGAGGGACAATTAATGTTGTAGAGGCTTGTGCTCAGACAGATACTGTTGATAAGATTGTCTTCACTTCCTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTATCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGTTGTGGTACCCTCTAGCAAAGACACTCTCTGAACAAGCTGCGTGGGCTCTAGCGATGGACCGTAGGCTCAAAATGGTGTCAATTAACGCAGGGCTTGTTCTTGGACCGGCTGTTGCAGAAGAAAATTCAGGGTCAACAATCTCATATCTTAAAGGAGCTGCTCAGATGTATGAAAATGGGGTGCTGGCAGTGGTTGATGTGAAGTTTTTGGTGGATGTTCATATCCGAGCAATGGAAGATTCGTCCACGGGTGGTCGATATTTCTGCTTCGATCAGATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTTCCACAAAGTTACGAAGTCCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACCAGGAGATTGCACAAACTGATCGAGGGCACAGCTTAA

Protein sequence

MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA
BLAST of Lsi06G010910 vs. Swiss-Prot
Match: CCR1_ARATH (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana GN=CCR1 PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 4.6e-35
Identity = 90/265 (33.96%), Postives = 134/265 (50.57%), Query Frame = 1

Query: 8   LCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIMD 67
           +CV  A  Y+  W+ K LL +GY V   V+N   P+    +RE+   +  L++   D+ D
Sbjct: 13  VCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDPK-NTHLRELEGGKERLILCKADLQD 72

Query: 68  YQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIVFTSSLTA 127
           Y+++  A+ GC G+F          E+MV+  V G   V+ A A+   V ++V TSS+ A
Sbjct: 73  YEALKAAIDGCDGVFHTASPVTDDPEQMVEPAVNGAKFVINAAAEAK-VKRVVITSSIGA 132

Query: 128 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAGL 187
                N   E  VDE CWSD +FC+  K WY   K ++EQAAW  A ++ + +V +N  L
Sbjct: 133 VYMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGVDLVVLNPVL 192

Query: 188 VLGPAVAEENSGS---TISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFC 247
           VLGP +    + S    + YL G+A+ Y N   A VDV+ +   H+   E  S  GRY  
Sbjct: 193 VLGPPLQPTINASLYHVLKYLTGSAKTYANLTQAYVDVRDVALAHVLVYEAPSASGRYLL 252

Query: 248 FDQIVNSEEETVKLANILRPLISLP 270
            +       E V++   L P   LP
Sbjct: 253 AES-ARHRGEVVEILAKLFPEYPLP 274

BLAST of Lsi06G010910 vs. Swiss-Prot
Match: CCR2_ARATH (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana GN=CCR2 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-34
Identity = 87/266 (32.71%), Postives = 134/266 (50.38%), Query Frame = 1

Query: 7   ILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIM 66
           ++CV  A  Y+  W+ K LL +GY V   V+N   P+    +RE+   +  L +   D++
Sbjct: 7   LVCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDPK-NNHLRELQGAKERLTLHSADLL 66

Query: 67  DYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIVFTSSLT 126
           DY+++   + GC G+F          E M++  V G   V++A A+   V ++VFTSS+ 
Sbjct: 67  DYEALCATIDGCDGVFHTASPMTDDPETMLEPAVNGAKFVIDAAAKAK-VKRVVFTSSIG 126

Query: 127 AAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAG 186
           A     N  ++  VDE CWSD +FC+  K WY   K L+EQ+AW  A  + + +V +N  
Sbjct: 127 AVYMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDLVVLNPV 186

Query: 187 LVLGPAVAEENSGSTI---SYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYF 246
           LVLGP +    + S +    YL G+A+ Y N     VDV+ +   H+   E  S  GRY 
Sbjct: 187 LVLGPPLQSAINASLVHILKYLTGSAKTYANLTQVYVDVRDVALGHVLVYEAPSASGRYI 246

Query: 247 CFDQIVNSEEETVKLANILRPLISLP 270
             +  ++  E    LA    P   LP
Sbjct: 247 LAETALHRGEVVEILAKFF-PEYPLP 269

BLAST of Lsi06G010910 vs. Swiss-Prot
Match: TKPR1_ARATH (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.4e-31
Identity = 79/288 (27.43%), Postives = 148/288 (51.39%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENG--- 60
           MD  K  +CV  AS ++  W+ K LL +GY V   V++ G    EK +  + K+E     
Sbjct: 1   MDQAKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGN---EKKLAHLWKLEGAKER 60

Query: 61  LVVFPVDIMDYQSILVALKGCSGLFCC----MDTPHVFDEKMVDLEVRGTINVVEACAQT 120
           L +   D+M+  S   A+ GC G+F      +      +E+++   + GT+NV+ +C + 
Sbjct: 61  LRLVKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKN 120

Query: 121 DTVDKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA 180
            ++ ++V TSS +    R++   +  +DE  W+  E C++ ++WY L+KTL+EQAAW  +
Sbjct: 121 PSLKRVVLTSSSSTVRIRDDFDPKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKFS 180

Query: 181 MDRRLKMVSINAGLVLGPAVAEE---NSGSTISYLKGAAQMYE-NGVLAVVDVKFLVDVH 240
            +  + +V++    ++GP++  +    +   +  LKG  + ++ +G +  V +  +   H
Sbjct: 181 EENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVARTH 240

Query: 241 IRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGR 278
           I   E  +  GRY C   ++ S EE V   +   P + +P+ +E   R
Sbjct: 241 IVVFEHEAAQGRYICSSNVI-SLEELVSFLSARYPSLPIPKRFEKLNR 284

BLAST of Lsi06G010910 vs. Swiss-Prot
Match: TKPR2_ARATH (Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana GN=TKPR2 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 7.1e-28
Identity = 79/292 (27.05%), Postives = 138/292 (47.26%), Query Frame = 1

Query: 10  VLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIMDYQ 69
           V   + ++  ++ K LL  G+ V   V+N    E    + E    +  L +   D+    
Sbjct: 6   VTGGTGFIASYIIKSLLELGHTVRTTVRNPRDEEKVGFLWEFQGAKQRLKILQADLTVEG 65

Query: 70  SILVALKGCSGLF-----CCMDTPHVFDEKMVDLEVRGTINVVEACAQTD-TVDKIVFTS 129
           S   A+ G  G+F       +   H   E +VD  ++GT NV+ +CA++  T+ +IV TS
Sbjct: 66  SFDEAVNGVDGVFHTASPVLVPQDHNIQETLVDPIIKGTTNVMSSCAKSKATLKRIVLTS 125

Query: 130 SLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSI 189
           S ++  +R +      ++E  WSD E+C++  LWY  AKTL E+ AW +A ++ L +V +
Sbjct: 126 SCSSIRYRFDATEASPLNESHWSDPEYCKRFNLWYGYAKTLGEREAWRIAEEKGLDLVVV 185

Query: 190 NAGLVLGPAVAEENSGSTISYL---KGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 249
           N   V+GP +  + + + +  L   KG A  Y N  +  V +  +V  H+ AME+    G
Sbjct: 186 NPSFVVGPLLGPKPTSTLLMILAIAKGLAGEYPNFTVGFVHIDDVVAAHVLAMEEPKASG 245

Query: 250 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKL 293
           R  C   + +   E ++L     P          +  +     + TR++H+L
Sbjct: 246 RIICSSSVAH-WSEIIELMRNKYPNYPFENKCSNKEGDNSPHSMDTRKIHEL 296

BLAST of Lsi06G010910 vs. Swiss-Prot
Match: DFRA_MALDO (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica GN=DFR PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.7e-27
Identity = 90/279 (32.26%), Postives = 141/279 (50.54%), Query Frame = 1

Query: 8   LCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIMD 67
           +CV  AS ++G W+   LL  GY V A V++    +  K + ++ K E  L ++  D+ D
Sbjct: 8   VCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKADLAD 67

Query: 68  YQSILVALKGCSGLFCCMDTPHVFDEKMVDLEV-----RGTINVVEACAQTDTVDKIVFT 127
             S   A++GCSG+F  + TP  F+ K  + EV      G +++++AC +  TV K+VFT
Sbjct: 68  EGSFDEAIQGCSGVF-HVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLVFT 127

Query: 128 SSLTAAVWRENILSEKDV-DERCWSDKEFCRKMKL--W-YPLAKTLSEQAAWALAMDRRL 187
           SS       E+   +K V DE  WSD EFCR +K+  W Y ++KTL+EQAAW  A +  +
Sbjct: 128 SSAGTVNVEEH---QKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNI 187

Query: 188 KMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYEN-GVLAVVDVKFLVDV---HIRAME 247
             ++I   LV+GP +      S I+ L    +   + G++       L D+   HI   E
Sbjct: 188 DFITIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYE 247

Query: 248 DSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQSYE 274
                GRY C      +  E VK+     P  ++P  ++
Sbjct: 248 HPKAEGRYICSSHDA-TIHELVKMLREKYPEYNIPTKFK 281

BLAST of Lsi06G010910 vs. TrEMBL
Match: A0A0A0LW90_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G277460 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 8.5e-161
Identity = 284/297 (95.62%), Postives = 291/297 (97.98%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVV 60
           MDH+KP LCVLDASSYVGFWVTK LLNKGYPVHAAVQNTGKPEIEKTIREMG+VENGL V
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIV 120
           F VDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V+KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQ YEVQGREAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of Lsi06G010910 vs. TrEMBL
Match: A0A061GQX0_THECC (NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao GN=TCM_038481 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.4e-123
Identity = 220/298 (73.83%), Postives = 256/298 (85.91%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTG-KPEIEKTIREMGKVENGLV 60
           MD  KP +CVLDAS+YVGFW+ K LL++GY VHAA+Q  G + EIE+ I+EM +VE+ L 
Sbjct: 1   MDQEKPTVCVLDASTYVGFWILKGLLSRGYTVHAALQRKGSETEIEEKIKEMERVEDRLA 60

Query: 61  VFPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKI 120
           VF VDI+DYQSILVALKGCS LFCC+D+P  +D+ +VDLEVRG INVVEACAQTDT++KI
Sbjct: 61  VFTVDILDYQSILVALKGCSALFCCLDSPDGYDDVIVDLEVRGAINVVEACAQTDTIEKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLK 180
           VF+SSLTAAVWRENI  EKDVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR L 
Sbjct: 121 VFSSSLTAAVWRENICLEKDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTG 240
           MVS+NAGLVLGP VA++N  ST+SYLKGAAQM+ENGVLA VDV FL DV+IRA EDSST 
Sbjct: 181 MVSVNAGLVLGPGVAQQNPRSTMSYLKGAAQMFENGVLAFVDVGFLADVNIRAFEDSSTC 240

Query: 241 GRYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           GRYFCF+QIVN+EEE VKLA  L PL+SLP  YE QG E +AERLRT++L+KL+EGTA
Sbjct: 241 GRYFCFNQIVNTEEEAVKLAQSLSPLLSLPSKYECQGSEVYAERLRTKKLNKLVEGTA 298

BLAST of Lsi06G010910 vs. TrEMBL
Match: A0A0D2P9S1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-120
Identity = 216/298 (72.48%), Postives = 251/298 (84.23%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTG-KPEIEKTIREMGKVENGLV 60
           MDH KP  CVLDAS+YVGFW+ K LLN+GY VHAA+Q  G + +I K I+EM +VE  L 
Sbjct: 1   MDHEKPAACVLDASTYVGFWILKGLLNRGYTVHAAIQIKGSETDIVKKIKEMERVEERLS 60

Query: 61  VFPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKI 120
           VF VD +DY SILVALKGCS LFCC+D P  +D+ +VDLEVRG INVVEACAQTD++ KI
Sbjct: 61  VFSVDTLDYHSILVALKGCSALFCCLDCPDGYDDLIVDLEVRGAINVVEACAQTDSMQKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLK 180
           VFTSSLTAA+WRENI S+ DVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR L 
Sbjct: 121 VFTSSLTAAIWRENICSQTDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTG 240
           MVS+NAGLV+GPAVA++N  ST+SYLKGAA+M+ENGVLAVVDVKFL DVHIRA EDSST 
Sbjct: 181 MVSVNAGLVVGPAVAQQNPCSTMSYLKGAAEMFENGVLAVVDVKFLADVHIRAFEDSSTI 240

Query: 241 GRYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           GRYFCF+QIV++EEE VKL   L PL+S    YE QG EA+AERLRT++L+KL+EGTA
Sbjct: 241 GRYFCFNQIVHTEEEAVKLVETLSPLLSSQPKYECQGSEAYAERLRTKKLNKLVEGTA 298

BLAST of Lsi06G010910 vs. TrEMBL
Match: A0A0B0PSP6_GOSAR (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Gossypium arboreum GN=F383_12950 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.5e-120
Identity = 216/298 (72.48%), Postives = 251/298 (84.23%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTG-KPEIEKTIREMGKVENGLV 60
           MDH KP  CVLDAS+YVGFW+ K LLN+GY VHAA+Q  G + +I K I+EM +VE  L 
Sbjct: 1   MDHEKPAACVLDASTYVGFWILKGLLNRGYTVHAAIQMKGSETDIVKKIKEMERVEERLS 60

Query: 61  VFPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKI 120
           VF VDI+DY SILVALKGCS LFCC+D P  +D+ +VDLEVRG INVVEACAQTD++ KI
Sbjct: 61  VFSVDILDYHSILVALKGCSALFCCLDCPDGYDDLIVDLEVRGAINVVEACAQTDSMQKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLK 180
           VFTSSLTAA+WRENI S+ DVDER WSD++FCRKMKLWY LAKTLSEQAAWALAMDR L 
Sbjct: 121 VFTSSLTAAIWRENICSQTDVDERFWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTG 240
           MVS+NAGLV+GPAVA++N  ST+SYLKGAA+M+ENGVLAVVDVKFL DVHIRA EDSST 
Sbjct: 181 MVSVNAGLVVGPAVAQQNPCSTVSYLKGAAEMFENGVLAVVDVKFLADVHIRAFEDSSTI 240

Query: 241 GRYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           GRYFCF+QIV++EEE VKL   L PL+S    YE QG EA+AERLRT++L+KL+EGTA
Sbjct: 241 GRYFCFNQIVHTEEEAVKLVETLSPLLSSQPKYECQGSEAYAERLRTKKLNKLVEGTA 298

BLAST of Lsi06G010910 vs. TrEMBL
Match: A0A0D2MWH7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.2e-120
Identity = 215/297 (72.39%), Postives = 250/297 (84.18%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVV 60
           MDH KP  CVLDAS+YVGFW+ K LLN+GY VHAA+Q   + +I K I+EM +VE  L V
Sbjct: 1   MDHEKPAACVLDASTYVGFWILKGLLNRGYTVHAAIQ-IKETDIVKKIKEMERVEERLSV 60

Query: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIV 120
           F VD +DY SILVALKGCS LFCC+D P  +D+ +VDLEVRG INVVEACAQTD++ KIV
Sbjct: 61  FSVDTLDYHSILVALKGCSALFCCLDCPDGYDDLIVDLEVRGAINVVEACAQTDSMQKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAA+WRENI S+ DVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR L M
Sbjct: 121 FTSSLTAAIWRENICSQTDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLNM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VS+NAGLV+GPAVA++N  ST+SYLKGAA+M+ENGVLAVVDVKFL DVHIRA EDSST G
Sbjct: 181 VSVNAGLVVGPAVAQQNPCSTMSYLKGAAEMFENGVLAVVDVKFLADVHIRAFEDSSTIG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCF+QIV++EEE VKL   L PL+S    YE QG EA+AERLRT++L+KL+EGTA
Sbjct: 241 RYFCFNQIVHTEEEAVKLVETLSPLLSSQPKYECQGSEAYAERLRTKKLNKLVEGTA 296

BLAST of Lsi06G010910 vs. TAIR10
Match: AT4G30470.1 (AT4G30470.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 399.4 bits (1025), Expect = 1.9e-111
Identity = 193/298 (64.77%), Postives = 240/298 (80.54%), Query Frame = 1

Query: 1   MDHTKPI--LCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGL 60
           MDH K     CVLDAS+YVGFW+ K+LL++GY VHAA++  G+ EIE+ IREM   E  L
Sbjct: 1   MDHDKSTSCCCVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEERL 60

Query: 61  VVFPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDK 120
           VV+ VD++DYQSILV+LK C+ +FCC+D+P  +DEK VDLEVRG INVVEAC +T++++K
Sbjct: 61  VVYDVDVLDYQSILVSLKTCNVVFCCLDSPEGYDEKEVDLEVRGAINVVEACGRTESIEK 120

Query: 121 IVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL 180
           IVF+SSLTA++WR+NI ++KDVDE+CWSD++FCR  KLW+ LAK LSE+AAWALAMDRRL
Sbjct: 121 IVFSSSLTASIWRDNIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKAAWALAMDRRL 180

Query: 181 KMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST 240
            MVSIN GLV+GP+VA+ N+  T+SYLKGAAQMYENGVLA VDVKFL DVHIRA ED S 
Sbjct: 181 NMVSINPGLVVGPSVAQHNARPTMSYLKGAAQMYENGVLAYVDVKFLADVHIRAFEDVSA 240

Query: 241 GGRYFCFDQIVNSEEETVKLANILRPLISLPQSY--EVQGREAFAERLRTRRLHKLIE 295
            GRYFCF+QIVN+EEE +KL   L PLI +P  Y  E+ G E + ERLR  +L KL+E
Sbjct: 241 CGRYFCFNQIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERLRNNKLSKLVE 298

BLAST of Lsi06G010910 vs. TAIR10
Match: AT2G23910.1 (AT2G23910.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 378.3 bits (970), Expect = 4.5e-105
Identity = 179/289 (61.94%), Postives = 232/289 (80.28%), Query Frame = 1

Query: 9   CVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIMDY 68
           CVLDAS+YVGFW+ K LL +GY VHAA++  G+  +E+ IR+M   E  L V+ VD++DY
Sbjct: 11  CVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEVYDVDVLDY 70

Query: 69  QSILVALKGCSGLFCCMDTPHVFDE-KMVDLEVRGTINVVEACAQTDTVDKIVFTSSLTA 128
           QSIL++L  C+ +FCC+D P  +DE K VDLEVRG INVVEACA+T++++KIVF+SSLTA
Sbjct: 71  QSILISLNNCNAVFCCLDNPEGYDEEKEVDLEVRGAINVVEACARTESIEKIVFSSSLTA 130

Query: 129 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMVSINAGL 188
           A+WR+NI ++KDVDE+CWSD +FC K KLW+ LAKT SE+AAWALAMDR + MVS+N GL
Sbjct: 131 AIWRDNIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAAWALAMDRMVNMVSVNPGL 190

Query: 189 VLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQ 248
           ++GP+VA+ N   T+SYLKGAAQMYENGVLA VDV+F+ DVHIRA ED+S  GRYFCF+Q
Sbjct: 191 IVGPSVAQHNPRPTMSYLKGAAQMYENGVLAYVDVEFVADVHIRAFEDTSACGRYFCFNQ 250

Query: 249 IVNSEEETVKLANILRPLISLPQSY--EVQGREAFAERLRTRRLHKLIE 295
           IVN+EEE +KL   L PLI +P  +  E+QG E + ERLR ++L+KL+E
Sbjct: 251 IVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLRNKKLNKLVE 299

BLAST of Lsi06G010910 vs. TAIR10
Match: AT5G58490.1 (AT5G58490.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 166.0 bits (419), Expect = 3.5e-41
Identity = 91/274 (33.21%), Postives = 155/274 (56.57%), Query Frame = 1

Query: 5   KPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVD 64
           + ++CV  AS  +G W+  +LL +GY VHA V+N    +  K +  +      L +F +D
Sbjct: 6   REVVCVTGASGCIGSWLVHQLLLRGYSVHATVKNLQDEKETKHLEGLEGAATRLHLFEMD 65

Query: 65  IMDYQSILVALKGCSGLF-----CCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKI 124
           ++ Y ++  A+ GCSG+F     C +D      ++++D  V+GTINV+ A A+  +V ++
Sbjct: 66  LLQYDTVSAAINGCSGVFHLASPCIVDEVQDPQKQLLDPAVKGTINVLTA-AKEASVKRV 125

Query: 125 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLK 184
           V TSS++A     N  ++K  +E CW+ +++CR+  LWYPL+KTL+E+AAW  A ++ L 
Sbjct: 126 VVTSSISAITPSPNWPADKIKNEECWAAEDYCRQNGLWYPLSKTLAEKAAWEFAEEKGLD 185

Query: 185 MVSINAGLVLGPAVAEENSGS---TISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDS 244
           +V +N G V+GP +    + S    +  L+G  + YEN  +  V  K +   HI   ED 
Sbjct: 186 VVVVNPGTVMGPVIPPSLNASMHMLLRLLQGCTETYENFFMGSVHFKDVALAHILVYEDP 245

Query: 245 STGGRYFCFDQIVNSEEETVKLANILRPLISLPQ 271
            + GR+ C + I +  +   K+A  L P  ++P+
Sbjct: 246 YSKGRHLCVEAISHYGDFVAKVAE-LYPNYNVPK 277

BLAST of Lsi06G010910 vs. TAIR10
Match: AT5G14700.1 (AT5G14700.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 160.2 bits (404), Expect = 1.9e-39
Identity = 97/270 (35.93%), Postives = 146/270 (54.07%), Query Frame = 1

Query: 7   ILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREM------GKVENGLVV 66
           ++CV    SY+G  + K LL  GY V   V     PE ++ + EM          N +  
Sbjct: 54  LVCVTGGVSYLGRAIVKRLLVHGYSVRIVVDC---PEDKEKVSEMEADAETASFSNMITS 113

Query: 67  FPVDIMDYQSILVALKGCSGLF---CCMDTPHV--FDEKMVDLEVRGTINVVEACAQTDT 126
               + +  S++ A  GC+G+F     +D   V  + + M +LE + + +V+EAC +T +
Sbjct: 114 VVSRLTEIDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEACTRTAS 173

Query: 127 VDKIVFTSSLTAAVWRENILSEKD---VDERCWSDKEFCRKMKLWYPLAKTLSEQAAWAL 186
           V K VFTSSL A  W++N  +  D   ++E  WSD++ C   KLWY L K  +E+AAW +
Sbjct: 174 VRKCVFTSSLLACAWQKNPCNSLDHSVINEESWSDEQLCIDNKLWYALGKLKAEKAAWRI 233

Query: 187 AMDRRLKMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHI-- 246
           A  + LK+ +I   L+ GP     NS ST++YLKGA +MY NG+LA +DV  L   H+  
Sbjct: 234 ADSKGLKLATICPALITGPDFFNRNSTSTLAYLKGAKEMYSNGLLATMDVNRLAKAHVCL 293

Query: 247 -RAMEDSSTGGRYFCFDQIVNSEEETVKLA 260
              + + +  GRY CFD I+ S +   KLA
Sbjct: 294 WEGLGNKTAFGRYICFDTIL-SRDGAEKLA 319

BLAST of Lsi06G010910 vs. TAIR10
Match: AT1G51410.1 (AT1G51410.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 159.1 bits (401), Expect = 4.3e-39
Identity = 94/272 (34.56%), Postives = 151/272 (55.51%), Query Frame = 1

Query: 8   LCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVVFPVDIMD 67
           +CV  AS Y+  W+ K LL +GY V A+V++   P   + +  +   E  L +F  ++++
Sbjct: 9   VCVTGASGYIASWIVKLLLLRGYTVKASVRDPNDPRKTEHLLALEGAEERLKLFKANLLE 68

Query: 68  YQSILVALKGCSGLFCCMDTPHVFDEK-----MVDLEVRGTINVVEACAQTDTVDKIVFT 127
             S   A+ GC G+F    +P   D K     ++D  V+GTINV+ +C +T +V ++V T
Sbjct: 69  EGSFDSAIDGCEGVFHTA-SPFYHDVKDPQAELLDPAVKGTINVLSSCLKTSSVKRVVLT 128

Query: 128 SSLTAAVWRENILS-EKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKMV 187
           SS+ A  +     + E  VDE  ++D ++CR  KLWY L+KTL+E AAW  A +  L++V
Sbjct: 129 SSIAAVAFNGMPRTPETIVDETWFADPDYCRASKLWYVLSKTLAENAAWKFAKENNLQLV 188

Query: 188 SINAGLVLGPAV---AEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST 247
           SIN  +V+GP +      ++ + +S +KG AQ + N     V+VK + + HI+A E+   
Sbjct: 189 SINPAMVIGPLLQPTLNTSAAAVLSLIKG-AQTFPNATFGWVNVKDVANAHIQAFENPDA 248

Query: 248 GGRYFCFDQIVNSEEETVKLANILRPLISLPQ 271
            GRY C  + V    E V + + L P   LP+
Sbjct: 249 DGRY-CLVERVAHYSEVVNILHDLYPDFQLPE 277

BLAST of Lsi06G010910 vs. NCBI nr
Match: gi|778660311|ref|XP_011656009.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 574.3 bits (1479), Expect = 1.2e-160
Identity = 284/297 (95.62%), Postives = 291/297 (97.98%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVV 60
           MDH+KP LCVLDASSYVGFWVTK LLNKGYPVHAAVQNTGKPEIEKTIREMG+VENGL V
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIV 120
           F VDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V+KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQ YEVQGREAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of Lsi06G010910 vs. NCBI nr
Match: gi|659086217|ref|XP_008443817.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 568.5 bits (1464), Expect = 6.7e-159
Identity = 283/297 (95.29%), Postives = 291/297 (97.98%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVV 60
           MD +KPILCVLDASSYVGFWVTK LL+KGYPVHAAVQNTGKPEIEKTIREM KVENGL+V
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNTGKPEIEKTIREMCKVENGLMV 60

Query: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIV 120
           F VDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V+KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQ YEVQG+EAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGKEAFAERLRTRKLHKLIEGTA 297

BLAST of Lsi06G010910 vs. NCBI nr
Match: gi|778660314|ref|XP_011656012.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 520.0 bits (1338), Expect = 2.7e-144
Identity = 263/297 (88.55%), Postives = 269/297 (90.57%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVV 60
           MDH+KP LCVLDASSYVGFWVTK LLNKGYPVHAAVQNT                     
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNT--------------------- 60

Query: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIV 120
              DIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V+KIV
Sbjct: 61  ---DIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQ YEVQGREAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 273

BLAST of Lsi06G010910 vs. NCBI nr
Match: gi|659086219|ref|XP_008443819.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 515.8 bits (1327), Expect = 5.1e-143
Identity = 262/297 (88.22%), Postives = 269/297 (90.57%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTGKPEIEKTIREMGKVENGLVV 60
           MD +KPILCVLDASSYVGFWVTK LL+KGYPVHAAVQNT                     
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNT--------------------- 60

Query: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKIV 120
              DIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V+KIV
Sbjct: 61  ---DIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQ YEVQG+EAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGKEAFAERLRTRKLHKLIEGTA 273

BLAST of Lsi06G010910 vs. NCBI nr
Match: gi|590579976|ref|XP_007013938.1| (NAD(P)-binding Rossmann-fold superfamily protein [Theobroma cacao])

HSP 1 Score: 449.9 bits (1156), Expect = 3.5e-123
Identity = 220/298 (73.83%), Postives = 256/298 (85.91%), Query Frame = 1

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNTG-KPEIEKTIREMGKVENGLV 60
           MD  KP +CVLDAS+YVGFW+ K LL++GY VHAA+Q  G + EIE+ I+EM +VE+ L 
Sbjct: 1   MDQEKPTVCVLDASTYVGFWILKGLLSRGYTVHAALQRKGSETEIEEKIKEMERVEDRLA 60

Query: 61  VFPVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVDKI 120
           VF VDI+DYQSILVALKGCS LFCC+D+P  +D+ +VDLEVRG INVVEACAQTDT++KI
Sbjct: 61  VFTVDILDYQSILVALKGCSALFCCLDSPDGYDDVIVDLEVRGAINVVEACAQTDTIEKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLK 180
           VF+SSLTAAVWRENI  EKDVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR L 
Sbjct: 121 VFSSSLTAAVWRENICLEKDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTG 240
           MVS+NAGLVLGP VA++N  ST+SYLKGAAQM+ENGVLA VDV FL DV+IRA EDSST 
Sbjct: 181 MVSVNAGLVLGPGVAQQNPRSTMSYLKGAAQMFENGVLAFVDVGFLADVNIRAFEDSSTC 240

Query: 241 GRYFCFDQIVNSEEETVKLANILRPLISLPQSYEVQGREAFAERLRTRRLHKLIEGTA 298
           GRYFCF+QIVN+EEE VKLA  L PL+SLP  YE QG E +AERLRT++L+KL+EGTA
Sbjct: 241 GRYFCFNQIVNTEEEAVKLAQSLSPLLSLPSKYECQGSEVYAERLRTKKLNKLVEGTA 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CCR1_ARATH4.6e-3533.96Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana GN=CCR1 PE=1 SV=1[more]
CCR2_ARATH1.3e-3432.71Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana GN=CCR2 PE=1 SV=1[more]
TKPR1_ARATH2.4e-3127.43Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana GN=TKPR1 PE=1 SV=1[more]
TKPR2_ARATH7.1e-2827.05Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana GN=TKPR2 PE=1 SV=1[more]
DFRA_MALDO2.7e-2732.26Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestic... [more]
Match NameE-valueIdentityDescription
A0A0A0LW90_CUCSA8.5e-16195.62Uncharacterized protein OS=Cucumis sativus GN=Csa_1G277460 PE=3 SV=1[more]
A0A061GQX0_THECC2.4e-12373.83NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao GN=TCM_03848... [more]
A0A0D2P9S1_GOSRA1.1e-12072.48Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1[more]
A0A0B0PSP6_GOSAR2.5e-12072.48Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Gossypium arbo... [more]
A0A0D2MWH7_GOSRA4.2e-12072.39Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G30470.11.9e-11164.77 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT2G23910.14.5e-10561.94 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G58490.13.5e-4133.21 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G14700.11.9e-3935.93 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT1G51410.14.3e-3934.56 NAD(P)-binding Rossmann-fold superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778660311|ref|XP_011656009.1|1.2e-16095.62PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis sativus][more]
gi|659086217|ref|XP_008443817.1|6.7e-15995.29PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo][more]
gi|778660314|ref|XP_011656012.1|2.7e-14488.55PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis sativus][more]
gi|659086219|ref|XP_008443819.1|5.1e-14388.22PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis melo][more]
gi|590579976|ref|XP_007013938.1|3.5e-12373.83NAD(P)-binding Rossmann-fold superfamily protein [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016040NAD(P)-bd_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008209 androgen metabolic process
biological_process GO:0008207 C21-steroid hormone metabolic process
biological_process GO:0008210 estrogen metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006694 steroid biosynthetic process
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0009117 nucleotide metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0050662 coenzyme binding
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0008253 5'-nucleotidase activity
molecular_function GO:0050379 UDP-glucuronate 5'-epimerase activity
molecular_function GO:0016857 racemase and epimerase activity, acting on carbohydrates and derivatives
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G010910.1Lsi06G010910.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 10..248
score: 1.2
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 13..128
score: 3.
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 6..251
score: 1.68
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 7..293
score: 4.8E
NoneNo IPR availablePANTHERPTHR10366:SF390CINNAMOYL-COA REDUCTASE LIKE PROTEINcoord: 7..293
score: 4.8E