CmoCh11G004330 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh11G004330
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Location	Cmo_Chr11 : 2123930 .. 2125506 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCACAGAAGGCCAACTCTTTGTGTTCTCGACGCTTCCTCTTATGTTGGCTTATGGGTCACCAAAGGATTGATAAACAGAGGATACCCAGTTCATGCAGCCGTTCAGAAAACGGGTAAGTCATTTATTCTTCTCTGTTCTTTTGCAATTCTTTTTCTTCACGAAGGCTTTCTAATGCTCTGCTTCTGGGTGAAATTATCGGTTTGATCGAGCAGGAAATCCAGAGATTGAGAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGGCTGGTGGTGTTACCTGTGGATATTATGGATTACCAGAGCATTCTTGTTGCTTTGAAAGGGTGCTCTGGTCTGTTTTGCTGTATGGACACCCCCCATGTGTATGATGTAAGTCCACAGAGGCAAAAAAAGGCTGATGATGCTTAAAGTTGTCATTTTCTTTCTCGGGAATTTGTGGGTTATTGTTGCTTTTGTGTTTTGTGAAGGAAAAAATGGTGGATTTGGAGGTGAGAGGGACAATTAATGTTGTGGAGGCCTGTGCTCGGACAGATACTGTTGAGAAGATAGTCTTCACCTCCTCCTTGACTGCTGCCGTATGGAGAGAGAATATTCTTTCTGAGAAGGATGTGGATGAAAGGTGTTGGAGTGATAAAGAATTTTGCAGAAAAATGAAGGTTGGAAGGAATTTCAAAACCTCTGTTATCTTTTTGAGTTGGTAATTTTGGATTTCTTCATTGGGCTATGTTAATCAACGAAGCCCTTATCATTCATTTTTCTTGTCAATGAATTTGATTACAAATCAAGCTCTGTTACGTTCTCCCAGTTGTGGTATCCTCTAGCAAAGACACTCTCTGAACAAGCAGCCTGGGCTCTAGCAATGGACCGTAGGCTGAACATGGTGTCCATTAACGCAGGACTTGTTCTTGGACCCGCTGTTGCAGAAGAAAATTCAGTGTCAACAATCTCATATCTTAAAGGTAGGAGGAGAAACTTCTTTAAAGACTGTTCCACATTTGTGGGAGTACTTGTCTTGTTTTGCTTAGCTGAAAATTATGAGACAACAGGAGCCGCTGAAATGTATGAAAATGGGGTTCTGGCAGTGGTGGATGTGAAGTTTTTGGTGGATGTCCATATCCGAGCAATGGAAGATTCCTCGACGGGTGGTCGGTACTTCTGCTTCGATCAGATAGTGAATAGTGAGGATGAAACTGTTAAACTTGCGAATACATTGAGGCCATTGATATCCATTCCACCGAGGTAAAAGAATTTGGGGTTTTTAAACAACAAACAGAGCAATAACATTGGAAACTGAATCTGTGTTAGTTTATTGGTGAGAGCAGGTGCGAAGGGCAAGGGAGGGAAGGTTTTGCTGAGAGGCTGAGAAGCAGGAGATTGCACAAACTGATAGAGGGCGCAGCTTCTTGATAACTTTTGAATGTAAAAACCTTTTGGTTTTCTTGTATGCATCTCTCGCTTCCCTCTTTCTCTCTTCTACCTCTCATCTTCTTCTTCTACTTCTTCCTCTGATGTAAAACCTTTTTGATTTCACATATTCAGAGCTACGGAGAGAGAGAGAGAGAGAGAGAGA

mRNA sequence

ATGGATCACAGAAGGCCAACTCTTTGTGTTCTCGACGCTTCCTCTTATGTTGGCTTATGGGTCACCAAAGGATTGATAAACAGAGGATACCCAGTTCATGCAGCCGTTCAGAAAACGGGAAATCCAGAGATTGAGAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGGCTGGTGGTGTTACCTGTGGATATTATGGATTACCAGAGCATTCTTGTTGCTTTGAAAGGGTGCTCTGGTCTGTTTTGCTGTATGGACACCCCCCATGTGTATGATGAAAAAATGGTGGATTTGGAGGTGAGAGGGACAATTAATGTTGTGGAGGCCTGTGCTCGGACAGATACTGTTGAGAAGATAGTCTTCACCTCCTCCTTGACTGCTGCCGTATGGAGAGAGAATATTCTTTCTGAGAAGGATGTGGATGAAAGGTGTTGGAGTGATAAAGAATTTTGCAGAAAAATGAAGTTGTGGTATCCTCTAGCAAAGACACTCTCTGAACAAGCAGCCTGGGCTCTAGCAATGGACCGTAGGCTGAACATGGTGTCCATTAACGCAGGACTTGTTCTTGGACCCGCTGTTGCAGAAGAAAATTCAGTGTCAACAATCTCATATCTTAAAGGTAGGAGGAGAAACTTCTTTAAAGACTGTTCCACATTTGTGGGAGTACTTGTCTTGTTTTGCTTAGCTGAAAATTATGAGACAACAGGAGCCGCTGAAATGTATGAAAATGGGGTTCTGGCAGTGGTGGATGTGAAGTTTTTGGTGGATGTCCATATCCGAGCAATGGAAGATTCCTCGACGGGTGGTCGGTACTTCTGCTTCGATCAGATAGTGAATAGTGAGGATGAAACTGTTAAACTTGCGAATACATTGAGGCCATTGATATCCATTCCACCGAGGTGCGAAGGGCAAGGGAGGGAAGGTTTTGCTGAGAGGCTGAGAAGCAGGAGATTGCACAAACTGATAGAGGGCGCAGCTTCTTGATAACTTTTGAATGTAAAAACCTTTTGGTTTTCTTGTATGCATCTCTCGCTTCCCTCTTTCTCTCTTCTACCTCTCATCTTCTTCTTCTACTTCTTCCTCTGATGTAAAACCTTTTTGATTTCACATATTCAGAGCTACGGAGAGAGAGAGAGAGAGAGAGAGA

Coding sequence (CDS)

ATGGATCACAGAAGGCCAACTCTTTGTGTTCTCGACGCTTCCTCTTATGTTGGCTTATGGGTCACCAAAGGATTGATAAACAGAGGATACCCAGTTCATGCAGCCGTTCAGAAAACGGGAAATCCAGAGATTGAGAAGACTATTAGAGAGATGGGTAAAGTGGAGAATGGGCTGGTGGTGTTACCTGTGGATATTATGGATTACCAGAGCATTCTTGTTGCTTTGAAAGGGTGCTCTGGTCTGTTTTGCTGTATGGACACCCCCCATGTGTATGATGAAAAAATGGTGGATTTGGAGGTGAGAGGGACAATTAATGTTGTGGAGGCCTGTGCTCGGACAGATACTGTTGAGAAGATAGTCTTCACCTCCTCCTTGACTGCTGCCGTATGGAGAGAGAATATTCTTTCTGAGAAGGATGTGGATGAAAGGTGTTGGAGTGATAAAGAATTTTGCAGAAAAATGAAGTTGTGGTATCCTCTAGCAAAGACACTCTCTGAACAAGCAGCCTGGGCTCTAGCAATGGACCGTAGGCTGAACATGGTGTCCATTAACGCAGGACTTGTTCTTGGACCCGCTGTTGCAGAAGAAAATTCAGTGTCAACAATCTCATATCTTAAAGGTAGGAGGAGAAACTTCTTTAAAGACTGTTCCACATTTGTGGGAGTACTTGTCTTGTTTTGCTTAGCTGAAAATTATGAGACAACAGGAGCCGCTGAAATGTATGAAAATGGGGTTCTGGCAGTGGTGGATGTGAAGTTTTTGGTGGATGTCCATATCCGAGCAATGGAAGATTCCTCGACGGGTGGTCGGTACTTCTGCTTCGATCAGATAGTGAATAGTGAGGATGAAACTGTTAAACTTGCGAATACATTGAGGCCATTGATATCCATTCCACCGAGGTGCGAAGGGCAAGGGAGGGAAGGTTTTGCTGAGAGGCTGAGAAGCAGGAGATTGCACAAACTGATAGAGGGCGCAGCTTCTTGA

Homology

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BLAST of CmoCh11G004330 vs. Swiss-Prot
Match: CCR1_ARATH (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana GN=CCR1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 91/298 (30.54%), Postives = 140/298 (46.98%), Query Frame = 1


Query: 7   TLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVVLPVDIM 66
           T+CV  A  Y+  W+ K L+ RGY V   V+   +P+    +RE+   +  L++   D+ 
Sbjct: 12  TVCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDPK-NTHLRELEGGKERLILCKADLQ 71

Query: 67  DYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIVFTSSLT 126
           DY+++  A+ GC G+F          E+MV+  V G   V+ A A    V+++V TSS+ 
Sbjct: 72  DYEALKAAIDGCDGVFHTASPVTDDPEQMVEPAVNGAKFVINAAAEAK-VKRVVITSSIG 131

Query: 127 AAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNMVSINAG 186
           A     N   E  VDE CWSD +FC+  K WY   K ++EQAAW  A ++ +++V +N  
Sbjct: 132 AVYMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGVDLVVLNPV 191

Query: 187 LVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVL 246
           LVLGP +    + S    LK                            TG+A+ Y N   
Sbjct: 192 LVLGPPLQPTINASLYHVLK--------------------------YLTGSAKTYANLTQ 251

Query: 247 AVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPRCEGQ 305
           A VDV+ +   H+   E  S  GRY   +       E V++   L P   +P +C+ +
Sbjct: 252 AYVDVRDVALAHVLVYEAPSASGRYLLAES-ARHRGEVVEILAKLFPEYPLPTKCKDE 280

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BLAST of CmoCh11G004330 vs. Swiss-Prot
Match: CCR2_ARATH (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana GN=CCR2 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 86/297 (28.96%), Postives = 140/297 (47.14%), Query Frame = 1


Query: 8   LCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVVLPVDIMD 67
           +CV  A  Y+  W+ K L+ RGY V   V+   +P+    +RE+   +  L +   D++D
Sbjct: 8   VCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDPK-NNHLRELQGAKERLTLHSADLLD 67

Query: 68  YQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIVFTSSLTA 127
           Y+++   + GC G+F          E M++  V G   V++A A+   V+++VFTSS+ A
Sbjct: 68  YEALCATIDGCDGVFHTASPMTDDPETMLEPAVNGAKFVIDAAAKAK-VKRVVFTSSIGA 127

Query: 128 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNMVSINAGL 187
                N  ++  VDE CWSD +FC+  K WY   K L+EQ+AW  A  + +++V +N  L
Sbjct: 128 VYMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDLVVLNPVL 187

Query: 188 VLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLA 247
           VLGP +    + S +  LK                            TG+A+ Y N    
Sbjct: 188 VLGPPLQSAINASLVHILK--------------------------YLTGSAKTYANLTQV 247

Query: 248 VVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPRCEGQ 305
            VDV+ +   H+   E  S  GRY   +  ++   E V++     P   +P +C  +
Sbjct: 248 YVDVRDVALGHVLVYEAPSASGRYILAETALH-RGEVVEILAKFFPEYPLPTKCSDE 275

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BLAST of CmoCh11G004330 vs. Swiss-Prot
Match: TKPR1_ARATH (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.4e-30
Identity = 84/314 (26.75%), Postives = 151/314 (48.09%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENG--- 60
           MD  +  +CV  AS ++  W+ K L+  GY V   V+  GN   EK +  + K+E     
Sbjct: 1   MDQAKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGN---EKKLAHLWKLEGAKER 60

Query: 61  LVVLPVDIMDYQSILVALKGCSGLFCC----MDTPHVYDEKMVDLEVRGTINVVEACART 120
           L ++  D+M+  S   A+ GC G+F      +      +E+++   + GT+NV+ +C + 
Sbjct: 61  LRLVKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKN 120

Query: 121 DTVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA 180
            +++++V TSS +    R++   +  +DE  W+  E C++ ++WY L+KTL+EQAAW  +
Sbjct: 121 PSLKRVVLTSSSSTVRIRDDFDPKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKFS 180

Query: 181 MDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYE 240
            +  +++V++    ++GP++  +                   CST   VL L        
Sbjct: 181 EENGIDLVTVLPSFLVGPSLPPDL------------------CSTASDVLGLL------- 240

Query: 241 TTGAAEMYE-NGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLR 300
             G  E ++ +G +  V +  +   HI   E  +  GRY C   +++ E E V   +   
Sbjct: 241 -KGETEKFQWHGQMGYVHIDDVARTHIVVFEHEAAQGRYICSSNVISLE-ELVSFLSARY 284

Query: 301 PLISIPPRCEGQGR 307
           P + IP R E   R
Sbjct: 301 PSLPIPKRFEKLNR 284

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BLAST of CmoCh11G004330 vs. Swiss-Prot
Match: TKPR2_ARATH (Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana GN=TKPR2 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 80/318 (25.16%), Postives = 144/318 (45.28%), Query Frame = 1


Query: 10  VLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVVLPVDIMDYQ 69
           V   + ++  ++ K L+  G+ V   V+   + E    + E    +  L +L  D+    
Sbjct: 6   VTGGTGFIASYIIKSLLELGHTVRTTVRNPRDEEKVGFLWEFQGAKQRLKILQADLTVEG 65

Query: 70  SILVALKGCSGLF-----CCMDTPHVYDEKMVDLEVRGTINVVEACARTD-TVEKIVFTS 129
           S   A+ G  G+F       +   H   E +VD  ++GT NV+ +CA++  T+++IV TS
Sbjct: 66  SFDEAVNGVDGVFHTASPVLVPQDHNIQETLVDPIIKGTTNVMSSCAKSKATLKRIVLTS 125

Query: 130 SLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNMVSI 189
           S ++  +R +      ++E  WSD E+C++  LWY  AKTL E+ AW +A ++ L++V +
Sbjct: 126 SCSSIRYRFDATEASPLNESHWSDPEYCKRFNLWYGYAKTLGEREAWRIAEEKGLDLVVV 185

Query: 190 NAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEMYEN 249
           N   V+GP +                    K  ST + +L +          G A  Y N
Sbjct: 186 NPSFVVGPLLGP------------------KPTSTLLMILAI--------AKGLAGEYPN 245

Query: 250 GVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPRCEG 309
             +  V +  +V  H+ AME+    GR  C   + +   E ++L     P      +C  
Sbjct: 246 FTVGFVHIDDVVAAHVLAMEEPKASGRIICSSSVAH-WSEIIELMRNKYPNYPFENKCSN 296

Query: 310 QGREGFAERLRSRRLHKL 322
           +  +     + +R++H+L
Sbjct: 306 KEGDNSPHSMDTRKIHEL 296

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BLAST of CmoCh11G004330 vs. Swiss-Prot
Match: DFRA_MALDO (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica GN=DFR PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-27
Identity = 95/312 (30.45%), Postives = 146/312 (46.79%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           M     ++CV  AS ++G W+   L+  GY V A V+   N +  K + ++ K E  L +
Sbjct: 1   MGSESESVCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTL 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEV-----RGTINVVEACARTDT 120
              D+ D  S   A++GCSG+F  + TP  ++ K  + EV      G +++++AC +  T
Sbjct: 61  WKADLADEGSFDEAIQGCSGVF-HVATPMDFESKDPENEVIKPTINGLLDILKACQKAKT 120

Query: 121 VEKIVFTSSLTAAVWRENILSEKDV-DERCWSDKEFCRKMKL--W-YPLAKTLSEQAAWA 180
           V K+VFTSS       E+   +K V DE  WSD EFCR +K+  W Y ++KTL+EQAAW 
Sbjct: 121 VRKLVFTSSAGTVNVEEH---QKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWK 180

Query: 181 LAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAEN 240
            A +  ++ ++I   LV+GP +      S I+ L    RN      +  G++        
Sbjct: 181 YAKENNIDFITIIPTLVIGPFLMPSMPPSLITGLSPILRN-----ESHYGII-------- 240

Query: 241 YETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTL 300
                     + G    +D   L   HI   E     GRY C         E VK+    
Sbjct: 241 ----------KQGQYVHLDDLCL--SHIYLYEHPKAEGRYICSSHDATIH-ELVKMLREK 282

Query: 301 RPLISIPPRCEG 304
            P  +IP + +G
Sbjct: 301 YPEYNIPTKFKG 282

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BLAST of CmoCh11G004330 vs. TrEMBL
Match: A0A0A0LW90_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G277460 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.8e-149
Identity = 271/326 (83.13%), Postives = 281/326 (86.20%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           MDH +PTLCVLDASSYVG WVTKGL+N+GYPVHAAVQ TG PEIEKTIREMG+VENGL V
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIV 120
             VDIMDYQSILVALKGCSGLFCCMDT HVYDEKMVDLEVRGTINVVEACA+TD+VEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL M
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEM 240
           VSINAGLVLGPAVAEENS STISYLK                             GAA+M
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLK-----------------------------GAAQM 240

Query: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPR 300
           YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSE+ETVKLAN LRPLISIP R
Sbjct: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISIPQR 297

Query: 301 CEGQGREGFAERLRSRRLHKLIEGAA 327
            E QGRE FAERLR+R+LHKLIEG A
Sbjct: 301 YEVQGREAFAERLRTRKLHKLIEGTA 297

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BLAST of CmoCh11G004330 vs. TrEMBL
Match: A0A061GQX0_THECC (NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao GN=TCM_038481 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.3e-117
Identity = 215/327 (65.75%), Postives = 258/327 (78.90%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNP-EIEKTIREMGKVENGLV 60
           MD  +PT+CVLDAS+YVG W+ KGL++RGY VHAA+Q+ G+  EIE+ I+EM +VE+ L 
Sbjct: 1   MDQEKPTVCVLDASTYVGFWILKGLLSRGYTVHAALQRKGSETEIEEKIKEMERVEDRLA 60

Query: 61  VLPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKI 120
           V  VDI+DYQSILVALKGCS LFCC+D+P  YD+ +VDLEVRG INVVEACA+TDT+EKI
Sbjct: 61  VFTVDILDYQSILVALKGCSALFCCLDSPDGYDDVIVDLEVRGAINVVEACAQTDTIEKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLN 180
           VF+SSLTAAVWRENI  EKDVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR LN
Sbjct: 121 VFSSSLTAAVWRENICLEKDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAE 240
           MVS+NAGLVLGP VA++N  ST+SYLK                             GAA+
Sbjct: 181 MVSVNAGLVLGPGVAQQNPRSTMSYLK-----------------------------GAAQ 240

Query: 241 MYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPP 300
           M+ENGVLA VDV FL DV+IRA EDSST GRYFCF+QIVN+E+E VKLA +L PL+S+P 
Sbjct: 241 MFENGVLAFVDVGFLADVNIRAFEDSSTCGRYFCFNQIVNTEEEAVKLAQSLSPLLSLPS 298

Query: 301 RCEGQGREGFAERLRSRRLHKLIEGAA 327
           + E QG E +AERLR+++L+KL+EG A
Sbjct: 301 KYECQGSEVYAERLRTKKLNKLVEGTA 298

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BLAST of CmoCh11G004330 vs. TrEMBL
Match: A0A0D2P9S1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.4e-116
Identity = 213/327 (65.14%), Postives = 252/327 (77.06%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNP-EIEKTIREMGKVENGLV 60
           MDH +P  CVLDAS+YVG W+ KGL+NRGY VHAA+Q  G+  +I K I+EM +VE  L 
Sbjct: 1   MDHEKPAACVLDASTYVGFWILKGLLNRGYTVHAAIQIKGSETDIVKKIKEMERVEERLS 60

Query: 61  VLPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKI 120
           V  VD +DY SILVALKGCS LFCC+D P  YD+ +VDLEVRG INVVEACA+TD+++KI
Sbjct: 61  VFSVDTLDYHSILVALKGCSALFCCLDCPDGYDDLIVDLEVRGAINVVEACAQTDSMQKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLN 180
           VFTSSLTAA+WRENI S+ DVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR LN
Sbjct: 121 VFTSSLTAAIWRENICSQTDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAE 240
           MVS+NAGLV+GPAVA++N  ST+SYLK                             GAAE
Sbjct: 181 MVSVNAGLVVGPAVAQQNPCSTMSYLK-----------------------------GAAE 240

Query: 241 MYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPP 300
           M+ENGVLAVVDVKFL DVHIRA EDSST GRYFCF+QIV++E+E VKL  TL PL+S  P
Sbjct: 241 MFENGVLAVVDVKFLADVHIRAFEDSSTIGRYFCFNQIVHTEEEAVKLVETLSPLLSSQP 298

Query: 301 RCEGQGREGFAERLRSRRLHKLIEGAA 327
           + E QG E +AERLR+++L+KL+EG A
Sbjct: 301 KYECQGSEAYAERLRTKKLNKLVEGTA 298

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BLAST of CmoCh11G004330 vs. TrEMBL
Match: A0A0B0PSP6_GOSAR (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Gossypium arboreum GN=F383_12950 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.4e-116
Identity = 213/327 (65.14%), Postives = 252/327 (77.06%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNP-EIEKTIREMGKVENGLV 60
           MDH +P  CVLDAS+YVG W+ KGL+NRGY VHAA+Q  G+  +I K I+EM +VE  L 
Sbjct: 1   MDHEKPAACVLDASTYVGFWILKGLLNRGYTVHAAIQMKGSETDIVKKIKEMERVEERLS 60

Query: 61  VLPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKI 120
           V  VDI+DY SILVALKGCS LFCC+D P  YD+ +VDLEVRG INVVEACA+TD+++KI
Sbjct: 61  VFSVDILDYHSILVALKGCSALFCCLDCPDGYDDLIVDLEVRGAINVVEACAQTDSMQKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLN 180
           VFTSSLTAA+WRENI S+ DVDER WSD++FCRKMKLWY LAKTLSEQAAWALAMDR LN
Sbjct: 121 VFTSSLTAAIWRENICSQTDVDERFWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAE 240
           MVS+NAGLV+GPAVA++N  ST+SYLK                             GAAE
Sbjct: 181 MVSVNAGLVVGPAVAQQNPCSTVSYLK-----------------------------GAAE 240

Query: 241 MYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPP 300
           M+ENGVLAVVDVKFL DVHIRA EDSST GRYFCF+QIV++E+E VKL  TL PL+S  P
Sbjct: 241 MFENGVLAVVDVKFLADVHIRAFEDSSTIGRYFCFNQIVHTEEEAVKLVETLSPLLSSQP 298

Query: 301 RCEGQGREGFAERLRSRRLHKLIEGAA 327
           + E QG E +AERLR+++L+KL+EG A
Sbjct: 301 KYECQGSEAYAERLRTKKLNKLVEGTA 298

[Close]

BLAST of CmoCh11G004330 vs. TrEMBL
Match: A0A0D2MWH7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 7.0e-116
Identity = 212/326 (65.03%), Postives = 250/326 (76.69%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           MDH +P  CVLDAS+YVG W+ KGL+NRGY VHAA+Q     +I K I+EM +VE  L V
Sbjct: 1   MDHEKPAACVLDASTYVGFWILKGLLNRGYTVHAAIQ-IKETDIVKKIKEMERVEERLSV 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIV 120
             VD +DY SILVALKGCS LFCC+D P  YD+ +VDLEVRG INVVEACA+TD+++KIV
Sbjct: 61  FSVDTLDYHSILVALKGCSALFCCLDCPDGYDDLIVDLEVRGAINVVEACAQTDSMQKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180
           FTSSLTAA+WRENI S+ DVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR LNM
Sbjct: 121 FTSSLTAAIWRENICSQTDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLNM 180

Query: 181 VSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEM 240
           VS+NAGLV+GPAVA++N  ST+SYLK                             GAAEM
Sbjct: 181 VSVNAGLVVGPAVAQQNPCSTMSYLK-----------------------------GAAEM 240

Query: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPR 300
           +ENGVLAVVDVKFL DVHIRA EDSST GRYFCF+QIV++E+E VKL  TL PL+S  P+
Sbjct: 241 FENGVLAVVDVKFLADVHIRAFEDSSTIGRYFCFNQIVHTEEEAVKLVETLSPLLSSQPK 296

Query: 301 CEGQGREGFAERLRSRRLHKLIEGAA 327
            E QG E +AERLR+++L+KL+EG A
Sbjct: 301 YECQGSEAYAERLRTKKLNKLVEGTA 296

[Close]

BLAST of CmoCh11G004330 vs. TAIR10
Match: AT4G30470.1 (AT4G30470.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 390.6 bits (1002), Expect = 9.6e-109
Identity = 195/331 (58.91%), Postives = 244/331 (73.72%), Query Frame = 1


Query: 1   MDHRRPT--LCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGL 60
           MDH + T   CVLDAS+YVG W+ K L++RGY VHAA+++ G  EIE+ IREM   E  L
Sbjct: 1   MDHDKSTSCCCVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEERL 60

Query: 61  VVLPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEK 120
           VV  VD++DYQSILV+LK C+ +FCC+D+P  YDEK VDLEVRG INVVEAC RT+++EK
Sbjct: 61  VVYDVDVLDYQSILVSLKTCNVVFCCLDSPEGYDEKEVDLEVRGAINVVEACGRTESIEK 120

Query: 121 IVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL 180
           IVF+SSLTA++WR+NI ++KDVDE+CWSD++FCR  KLW+ LAK LSE+AAWALAMDRRL
Sbjct: 121 IVFSSSLTASIWRDNIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKAAWALAMDRRL 180

Query: 181 NMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAA 240
           NMVSIN GLV+GP+VA+ N+  T+SYLK                             GAA
Sbjct: 181 NMVSINPGLVVGPSVAQHNARPTMSYLK-----------------------------GAA 240

Query: 241 EMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIP 300
           +MYENGVLA VDVKFL DVHIRA ED S  GRYFCF+QIVN+E+E +KL  +L PLI +P
Sbjct: 241 QMYENGVLAYVDVKFLADVHIRAFEDVSACGRYFCFNQIVNTEEEALKLVESLSPLIPMP 300

Query: 301 PRCEGQ--GREGFAERLRSRRLHKLIEGAAS 328
           PR E +  G E + ERLR+ +L KL+E  ++
Sbjct: 301 PRYENEMHGSEVYEERLRNNKLSKLVEAGSA 302

[Close]

BLAST of CmoCh11G004330 vs. TAIR10
Match: AT2G23910.1 (AT2G23910.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-102
Identity = 184/318 (57.86%), Postives = 233/318 (73.27%), Query Frame = 1


Query: 9   CVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVVLPVDIMDY 68
           CVLDAS+YVG W+ K L+ RGY VHAA++K G   +E+ IR+M   E  L V  VD++DY
Sbjct: 11  CVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEVYDVDVLDY 70

Query: 69  QSILVALKGCSGLFCCMDTPHVYDE-KMVDLEVRGTINVVEACARTDTVEKIVFTSSLTA 128
           QSIL++L  C+ +FCC+D P  YDE K VDLEVRG INVVEACART+++EKIVF+SSLTA
Sbjct: 71  QSILISLNNCNAVFCCLDNPEGYDEEKEVDLEVRGAINVVEACARTESIEKIVFSSSLTA 130

Query: 129 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNMVSINAGL 188
           A+WR+NI ++KDVDE+CWSD +FC K KLW+ LAKT SE+AAWALAMDR +NMVS+N GL
Sbjct: 131 AIWRDNIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAAWALAMDRMVNMVSVNPGL 190

Query: 189 VLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLA 248
           ++GP+VA+ N   T+SYLK                             GAA+MYENGVLA
Sbjct: 191 IVGPSVAQHNPRPTMSYLK-----------------------------GAAQMYENGVLA 250

Query: 249 VVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPRCEG--QG 308
            VDV+F+ DVHIRA ED+S  GRYFCF+QIVN+E+E +KL  TL PLI +PPR E   QG
Sbjct: 251 YVDVEFVADVHIRAFEDTSACGRYFCFNQIVNTEEEALKLVQTLSPLIPMPPRHEKEMQG 299

Query: 309 REGFAERLRSRRLHKLIE 324
            E + ERLR+++L+KL+E
Sbjct: 311 SEVYEERLRNKKLNKLVE 299

[Close]

BLAST of CmoCh11G004330 vs. TAIR10
Match: AT5G58490.1 (AT5G58490.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 156.8 bits (395), Expect = 2.3e-38
Identity = 92/299 (30.77%), Postives = 152/299 (50.84%), Query Frame = 1


Query: 5   RPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVVLPVD 64
           R  +CV  AS  +G W+   L+ RGY VHA V+   + +  K +  +      L +  +D
Sbjct: 6   REVVCVTGASGCIGSWLVHQLLLRGYSVHATVKNLQDEKETKHLEGLEGAATRLHLFEMD 65

Query: 65  IMDYQSILVALKGCSGLF-----CCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKI 124
           ++ Y ++  A+ GCSG+F     C +D      ++++D  V+GTINV+ A A+  +V+++
Sbjct: 66  LLQYDTVSAAINGCSGVFHLASPCIVDEVQDPQKQLLDPAVKGTINVLTA-AKEASVKRV 125

Query: 125 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLN 184
           V TSS++A     N  ++K  +E CW+ +++CR+  LWYPL+KTL+E+AAW  A ++ L+
Sbjct: 126 VVTSSISAITPSPNWPADKIKNEECWAAEDYCRQNGLWYPLSKTLAEKAAWEFAEEKGLD 185

Query: 185 MVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAE 244
           +V +N G V+GP +    + S    L+                             G  E
Sbjct: 186 VVVVNPGTVMGPVIPPSLNASMHMLLR--------------------------LLQGCTE 245

Query: 245 MYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIP 299
            YEN  +  V  K +   HI   ED  + GR+ C + I +  D   K+A  L P  ++P
Sbjct: 246 TYENFFMGSVHFKDVALAHILVYEDPYSKGRHLCVEAISHYGDFVAKVAE-LYPNYNVP 276

[Close]

BLAST of CmoCh11G004330 vs. TAIR10
Match: AT1G51410.1 (AT1G51410.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 153.7 bits (387), Expect = 2.0e-37
Identity = 93/304 (30.59%), Postives = 153/304 (50.33%), Query Frame = 1


Query: 7   TLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVVLPVDIM 66
           T+CV  AS Y+  W+ K L+ RGY V A+V+   +P   + +  +   E  L +   +++
Sbjct: 8   TVCVTGASGYIASWIVKLLLLRGYTVKASVRDPNDPRKTEHLLALEGAEERLKLFKANLL 67

Query: 67  DYQSILVALKGCSGLFCCMDTPHVYDEK-----MVDLEVRGTINVVEACARTDTVEKIVF 126
           +  S   A+ GC G+F    +P  +D K     ++D  V+GTINV+ +C +T +V+++V 
Sbjct: 68  EEGSFDSAIDGCEGVFHTA-SPFYHDVKDPQAELLDPAVKGTINVLSSCLKTSSVKRVVL 127

Query: 127 TSSLTAAVWRENILS-EKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 186
           TSS+ A  +     + E  VDE  ++D ++CR  KLWY L+KTL+E AAW  A +  L +
Sbjct: 128 TSSIAAVAFNGMPRTPETIVDETWFADPDYCRASKLWYVLSKTLAENAAWKFAKENNLQL 187

Query: 187 VSINAGLVLGPAV---AEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGA 246
           VSIN  +V+GP +      ++ + +S +KG                              
Sbjct: 188 VSINPAMVIGPLLQPTLNTSAAAVLSLIKG------------------------------ 247

Query: 247 AEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISI 302
           A+ + N     V+VK + + HI+A E+    GRY C  + V    E V + + L P   +
Sbjct: 248 AQTFPNATFGWVNVKDVANAHIQAFENPDADGRY-CLVERVAHYSEVVNILHDLYPDFQL 279

[Close]

BLAST of CmoCh11G004330 vs. TAIR10
Match: AT5G14700.1 (AT5G14700.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 152.1 bits (383), Expect = 5.8e-37
Identity = 99/301 (32.89%), Postives = 146/301 (48.50%), Query Frame = 1


Query: 8   LCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREM------GKVENGLVVL 67
           +CV    SY+G  + K L+  GY V   V     PE ++ + EM          N +  +
Sbjct: 55  VCVTGGVSYLGRAIVKRLLVHGYSVRIVVDC---PEDKEKVSEMEADAETASFSNMITSV 114

Query: 68  PVDIMDYQSILVALKGCSGLF---CCMDTPHV--YDEKMVDLEVRGTINVVEACARTDTV 127
              + +  S++ A  GC+G+F     +D   V  Y + M +LE + + +V+EAC RT +V
Sbjct: 115 VSRLTEIDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEACTRTASV 174

Query: 128 EKIVFTSSLTAAVWRENILSEKD---VDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA 187
            K VFTSSL A  W++N  +  D   ++E  WSD++ C   KLWY L K  +E+AAW +A
Sbjct: 175 RKCVFTSSLLACAWQKNPCNSLDHSVINEESWSDEQLCIDNKLWYALGKLKAEKAAWRIA 234

Query: 188 MDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYE 247
             + L + +I   L+ GP     NS ST++YLK                           
Sbjct: 235 DSKGLKLATICPALITGPDFFNRNSTSTLAYLK--------------------------- 294

Query: 248 TTGAAEMYENGVLAVVDVKFLVDVHI---RAMEDSSTGGRYFCFDQIVNSEDETVKLANT 292
             GA EMY NG+LA +DV  L   H+     + + +  GRY CFD I+ S D   KLA  
Sbjct: 295 --GAKEMYSNGLLATMDVNRLAKAHVCLWEGLGNKTAFGRYICFDTIL-SRDGAEKLAKD 322

[Close]

BLAST of CmoCh11G004330 vs. NCBI nr
Match: gi|778660311|ref|XP_011656009.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 535.4 bits (1378), Expect = 6.9e-149
Identity = 271/326 (83.13%), Postives = 281/326 (86.20%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           MDH +PTLCVLDASSYVG WVTKGL+N+GYPVHAAVQ TG PEIEKTIREMG+VENGL V
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIV 120
             VDIMDYQSILVALKGCSGLFCCMDT HVYDEKMVDLEVRGTINVVEACA+TD+VEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL M
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEM 240
           VSINAGLVLGPAVAEENS STISYLK                             GAA+M
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLK-----------------------------GAAQM 240

Query: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPR 300
           YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSE+ETVKLAN LRPLISIP R
Sbjct: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISIPQR 297

Query: 301 CEGQGREGFAERLRSRRLHKLIEGAA 327
            E QGRE FAERLR+R+LHKLIEG A
Sbjct: 301 YEVQGREAFAERLRTRKLHKLIEGTA 297

[Close]

BLAST of CmoCh11G004330 vs. NCBI nr
Match: gi|659086217|ref|XP_008443817.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 523.9 bits (1348), Expect = 2.1e-145
Identity = 266/326 (81.60%), Postives = 279/326 (85.58%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           MD  +P LCVLDASSYVG WVTKGL+++GYPVHAAVQ TG PEIEKTIREM KVENGL+V
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNTGKPEIEKTIREMCKVENGLMV 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIV 120
             VDIMDYQSILVALKGCSGLFCCMDT HVYDEKMVDLEVRGTINVVEACA+TD+VEKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL M
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEM 240
           VSINAGLVLGPAVAEENS STISYLK                             GAA+M
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLK-----------------------------GAAQM 240

Query: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPR 300
           YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSE+ETVKLAN LRPLIS+P R
Sbjct: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQR 297

Query: 301 CEGQGREGFAERLRSRRLHKLIEGAA 327
            E QG+E FAERLR+R+LHKLIEG A
Sbjct: 301 YEVQGKEAFAERLRTRKLHKLIEGTA 297

[Close]

BLAST of CmoCh11G004330 vs. NCBI nr
Match: gi|778660314|ref|XP_011656012.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 485.3 bits (1248), Expect = 8.2e-134
Identity = 252/326 (77.30%), Postives = 261/326 (80.06%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           MDH +PTLCVLDASSYVG WVTKGL+N+GYPVHAAVQ T                     
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNT--------------------- 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIV 120
              DIMDYQSILVALKGCSGLFCCMDT HVYDEKMVDLEVRGTINVVEACA+TD+VEKIV
Sbjct: 61  ---DIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL M
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEM 240
           VSINAGLVLGPAVAEENS STISYLK                             GAA+M
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLK-----------------------------GAAQM 240

Query: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPR 300
           YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSE+ETVKLAN LRPLISIP R
Sbjct: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISIPQR 273

Query: 301 CEGQGREGFAERLRSRRLHKLIEGAA 327
            E QGRE FAERLR+R+LHKLIEG A
Sbjct: 301 YEVQGREAFAERLRTRKLHKLIEGTA 273

[Close]

BLAST of CmoCh11G004330 vs. NCBI nr
Match: gi|659086219|ref|XP_008443819.1| (PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 475.3 bits (1222), Expect = 8.4e-131
Identity = 247/326 (75.77%), Postives = 259/326 (79.45%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNPEIEKTIREMGKVENGLVV 60
           MD  +P LCVLDASSYVG WVTKGL+++GYPVHAAVQ T                     
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNT--------------------- 60

Query: 61  LPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKIV 120
              DIMDYQSILVALKGCSGLFCCMDT HVYDEKMVDLEVRGTINVVEACA+TD+VEKIV
Sbjct: 61  ---DIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLNM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRL M
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEM 240
           VSINAGLVLGPAVAEENS STISYLK                             GAA+M
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLK-----------------------------GAAQM 240

Query: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPR 300
           YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSE+ETVKLAN LRPLIS+P R
Sbjct: 241 YENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQR 273

Query: 301 CEGQGREGFAERLRSRRLHKLIEGAA 327
            E QG+E FAERLR+R+LHKLIEG A
Sbjct: 301 YEVQGKEAFAERLRTRKLHKLIEGTA 273

[Close]

BLAST of CmoCh11G004330 vs. NCBI nr
Match: gi|590579976|ref|XP_007013938.1| (NAD(P)-binding Rossmann-fold superfamily protein [Theobroma cacao])

HSP 1 Score: 431.0 bits (1107), Expect = 1.8e-117
Identity = 215/327 (65.75%), Postives = 258/327 (78.90%), Query Frame = 1


Query: 1   MDHRRPTLCVLDASSYVGLWVTKGLINRGYPVHAAVQKTGNP-EIEKTIREMGKVENGLV 60
           MD  +PT+CVLDAS+YVG W+ KGL++RGY VHAA+Q+ G+  EIE+ I+EM +VE+ L 
Sbjct: 1   MDQEKPTVCVLDASTYVGFWILKGLLSRGYTVHAALQRKGSETEIEEKIKEMERVEDRLA 60

Query: 61  VLPVDIMDYQSILVALKGCSGLFCCMDTPHVYDEKMVDLEVRGTINVVEACARTDTVEKI 120
           V  VDI+DYQSILVALKGCS LFCC+D+P  YD+ +VDLEVRG INVVEACA+TDT+EKI
Sbjct: 61  VFTVDILDYQSILVALKGCSALFCCLDSPDGYDDVIVDLEVRGAINVVEACAQTDTIEKI 120

Query: 121 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLN 180
           VF+SSLTAAVWRENI  EKDVDERCWSD++FCRKMKLWY LAKTLSEQAAWALAMDR LN
Sbjct: 121 VFSSSLTAAVWRENICLEKDVDERCWSDQDFCRKMKLWYALAKTLSEQAAWALAMDRMLN 180

Query: 181 MVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAE 240
           MVS+NAGLVLGP VA++N  ST+SYLK                             GAA+
Sbjct: 181 MVSVNAGLVLGPGVAQQNPRSTMSYLK-----------------------------GAAQ 240

Query: 241 MYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPP 300
           M+ENGVLA VDV FL DV+IRA EDSST GRYFCF+QIVN+E+E VKLA +L PL+S+P 
Sbjct: 241 MFENGVLAFVDVGFLADVNIRAFEDSSTCGRYFCFNQIVNTEEEAVKLAQSLSPLLSLPS 298

Query: 301 RCEGQGREGFAERLRSRRLHKLIEGAA 327
           + E QG E +AERLR+++L+KL+EG A
Sbjct: 301 KYECQGSEVYAERLRTKKLNKLVEGTA 298

The following BLAST results are available for this feature:

BLAST of CmoCh11G004330 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CCR1_ARATH	1.5e-34	30.54	Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana GN=CCR1 PE=1 SV=1	[more]
CCR2_ARATH	2.1e-33	28.96	Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana GN=CCR2 PE=1 SV=1	[more]
TKPR1_ARATH	8.4e-30	26.75	Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana GN=TKPR1 PE=1 SV=1	[more]
TKPR2_ARATH	1.0e-27	25.16	Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana GN=TKPR2 PE=1 SV=1	[more]
DFRA_MALDO	1.8e-27	30.45	Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestic...	[more]

BLAST of CmoCh11G004330 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LW90_CUCSA	4.8e-149	83.13	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G277460 PE=3 SV=1	[more]
A0A061GQX0_THECC	1.3e-117	65.75	NAD(P)-binding Rossmann-fold superfamily protein OS=Theobroma cacao GN=TCM_03848...	[more]
A0A0D2P9S1_GOSRA	1.4e-116	65.14	Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1	[more]
A0A0B0PSP6_GOSAR	2.4e-116	65.14	Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Gossypium arbo...	[more]
A0A0D2MWH7_GOSRA	7.0e-116	65.03	Uncharacterized protein OS=Gossypium raimondii GN=B456_004G105500 PE=4 SV=1	[more]

BLAST of CmoCh11G004330 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G30470.1	9.6e-109	58.91	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G23910.1	1.3e-102	57.86	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G58490.1	2.3e-38	30.77	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G51410.1	2.0e-37	30.59	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G14700.1	5.8e-37	32.89	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of CmoCh11G004330 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778660311\|ref\|XP_011656009.1\|	6.9e-149	83.13	PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis sativus]	[more]
gi\|659086217\|ref\|XP_008443817.1\|	2.1e-145	81.60	PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo]	[more]
gi\|778660314\|ref\|XP_011656012.1\|	8.2e-134	77.30	PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis sativus]	[more]
gi\|659086219\|ref\|XP_008443819.1\|	8.4e-131	75.77	PREDICTED: cinnamoyl-CoA reductase 1 isoform X2 [Cucumis melo]	[more]
gi\|590579976\|ref\|XP_007013938.1\|	1.8e-117	65.75	NAD(P)-binding Rossmann-fold superfamily protein [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002225	3Beta_OHSteriod_DH/Estase
IPR016040	NAD(P)-bd_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006694	steroid biosynthetic process
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008209	androgen metabolic process
biological_process	GO:0008207	C21-steroid hormone metabolic process
biological_process	GO:0008210	estrogen metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006694	steroid biosynthetic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0050662	coenzyme binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G004330.1	CmoCh11G004330.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002225	3-beta hydroxysteroid dehydrogenase/isomerase	PFAM	PF01073	3Beta_HSD	coord: 11..168 score: 3.
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 9..277 score: 4.3
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 241..293 score: 2.85E-34coord: 6..191 score: 2.85
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 236..322 score: 9.2E-161coord: 7..206 score: 9.2E
None	No IPR available	PANTHER	PTHR10366:SF390	CINNAMOYL-COA REDUCTASE LIKE PROTEIN	coord: 236..322 score: 9.2E-161coord: 7..206 score: 9.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh11G004330	CmaCh10G004420	Cucurbita maxima (Rimu)	cmacmoB068
CmoCh11G004330	CmaCh11G004340	Cucurbita maxima (Rimu)	cmacmoB112
CmoCh11G004330	Cla018764	Watermelon (97103) v1	cmowmB128
CmoCh11G004330	Csa1G277460	Cucumber (Chinese Long) v2	cmocuB092
CmoCh11G004330	MELO3C010188	Melon (DHL92) v3.5.1	cmomeB098
CmoCh11G004330	ClCG06G012750	Watermelon (Charleston Gray)	cmowcgB119
CmoCh11G004330	CSPI01G18070	Wild cucumber (PI 183967)	cmocpiB090
CmoCh11G004330	Lsi06G010910	Bottle gourd (USVL1VR-Ls)	cmolsiB132
CmoCh11G004330	Cp4.1LG04g12730	Cucurbita pepo (Zucchini)	cmocpeB129
CmoCh11G004330	MELO3C010188.2	Melon (DHL92) v3.6.1	cmomedB105
CmoCh11G004330	CsaV3_1G029290	Cucumber (Chinese Long) v3	cmocucB0100
CmoCh11G004330	Cla97C06G121990	Watermelon (97103) v2	cmowmbB123
CmoCh11G004330	Bhi02G000648	Wax gourd	cmowgoB0145
CmoCh11G004330	CsGy1G018150	Cucumber (Gy14) v2	cgybcmoB037
CmoCh11G004330	Carg14062	Silver-seed gourd	carcmoB0689

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh11G004330	Bottle gourd (USVL1VR-Ls)	cmolsiB092
CmoCh11G004330	Cucumber (Gy14) v2	cgybcmoB157
CmoCh11G004330	Melon (DHL92) v3.6.1	cmomedB132
CmoCh11G004330	Silver-seed gourd	carcmoB0175
CmoCh11G004330	Cucumber (Chinese Long) v3	cmocucB0122
CmoCh11G004330	Watermelon (97103) v2	cmowmbB085
CmoCh11G004330	Wax gourd	cmowgoB0092
CmoCh11G004330	Cucurbita moschata (Rifu)	cmocmoB038
CmoCh11G004330	Cucurbita moschata (Rifu)	cmocmoB081
CmoCh11G004330	Cucumber (Gy14) v1	cgycmoB0172
CmoCh11G004330	Cucurbita maxima (Rimu)	cmacmoB172
CmoCh11G004330	Wild cucumber (PI 183967)	cmocpiB109
CmoCh11G004330	Cucumber (Chinese Long) v2	cmocuB106
CmoCh11G004330	Melon (DHL92) v3.5.1	cmomeB122
CmoCh11G004330	Watermelon (Charleston Gray)	cmowcgB082
CmoCh11G004330	Watermelon (97103) v1	cmowmB120
CmoCh11G004330	Cucurbita pepo (Zucchini)	cmocpeB108