Lsi06G006330 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G006330
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Aluminum-activated malate transporter 2
Location	chr06 : 9257895 .. 9258347 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACCCTGTTTGAAGCCATAGCTAAAATAGCAACTGACATTTGTTTCTACAGTTACACTTGGAAATTCAAAGGCAATACCAAGAACTCTGCTTACAACTGTGCACTGAATCAGGCAAAGCTTTAAAGTCCATAGCAATGGCAATAAGAGATATAATAATTTCACCTGCCTTGGCCAAGTCCCACATTAAAATAGCAAAGGAGAAGGCTGAGGAGCTCATGTCTTTTCTAACAACTAGCCATTTTAATGGTGACATGAAGATGGTATCAACTACAACTTTGATTTTACTGCTCATTGATTGTTTATCTTGTGTCGAGAAGATCGCTGATTCCATCCGCGAGTTTGTTTCGCTTGCTCGGCTCAAGACCGTGTTGAAGCAGGCCGGTGTAACGCCGACAGAGCAAAAGCCTCATCATAACATTACCATTACTCAAATTCAAATTGGAGAGTAG

mRNA sequence

ATGTACCCTTTACACTTGGAAATTCAAAGGCAATACCAAGAACTCTGCTTACAACTGTGCACTGAATCAGGCAAAGCTTTAAAGTCCATAGCAATGGCAATAAGAGATATAATAATTTCACCTGCCTTGGCCAAGTCCCACATTAAAATAGCAAAGGAGAAGGCTGAGGAGCTCATGTCTTTTCTAACAACTAGCCATTTTAATGGTGACATGAAGATGGTATCAACTACAACTTTGATTTTACTGCTCATTGATTGTTTATCTTGTGTCGAGAAGATCGCTGATTCCATCCGCGAGTTTGTTTCGCTTGCTCGGCTCAAGACCGTGTTGAAGCAGGCCGGTGTAACGCCGACAGAGCAAAAGCCTCATCATAACATTACCATTACTCAAATTCAAATTGGAGAGTAG

Coding sequence (CDS)

ATGTACCCTTTACACTTGGAAATTCAAAGGCAATACCAAGAACTCTGCTTACAACTGTGCACTGAATCAGGCAAAGCTTTAAAGTCCATAGCAATGGCAATAAGAGATATAATAATTTCACCTGCCTTGGCCAAGTCCCACATTAAAATAGCAAAGGAGAAGGCTGAGGAGCTCATGTCTTTTCTAACAACTAGCCATTTTAATGGTGACATGAAGATGGTATCAACTACAACTTTGATTTTACTGCTCATTGATTGTTTATCTTGTGTCGAGAAGATCGCTGATTCCATCCGCGAGTTTGTTTCGCTTGCTCGGCTCAAGACCGTGTTGAAGCAGGCCGGTGTAACGCCGACAGAGCAAAAGCCTCATCATAACATTACCATTACTCAAATTCAAATTGGAGAGTAG

Protein sequence

MYPLHLEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTSHFNGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVLKQAGVTPTEQKPHHNITITQIQIGE

Homology

[Close]

BLAST of Lsi06G006330 vs. TrEMBL
Match: A0A0A0LZX9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630340 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.0e-41
Identity = 101/132 (76.52%), Postives = 106/132 (80.30%), Query Frame = 1


Query: 6   LEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTS 65
           LEIQRQYQE CLQLC ESGKAL SIAMAIRDII  PA+AKSH + AKEKAE LMS L +S
Sbjct: 51  LEIQRQYQEHCLQLCIESGKALNSIAMAIRDII-PPAMAKSHTEKAKEKAEALMSLLKSS 110

Query: 66  HFNGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTV--LKQAGVTPTEQKPH 125
           HFNGDMKMVSTTTLI LLIDCLSCVEKI DS+ + VSLAR KT    KQAGV  TEQK  
Sbjct: 111 HFNGDMKMVSTTTLIYLLIDCLSCVEKIVDSVHDLVSLARPKTTHPPKQAGVMSTEQKAP 170

Query: 126 HNITITQIQIGE 136
           HNI IT IQI E
Sbjct: 171 HNIIITHIQIAE 181

[Close]

BLAST of Lsi06G006330 vs. TrEMBL
Match: A0A0A0M301_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630850 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.5e-13
Identity = 47/117 (40.17%), Postives = 73/117 (62.39%), Query Frame = 1


Query: 7   EIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTSH 66
           +I  Q++E C+++C+ES + L+ +A+A+R +++ P  AKSHI+ AK  AE L S L    
Sbjct: 323 QIHGQFKEECMKICSESSRGLRELALALRKMVL-PLTAKSHIEKAKIAAENLKSHLEEWR 382

Query: 67  F---NGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVLKQAGVTPTEQ 121
           F   N  M++V   +L  LL D + C+EKI DS++E  S+A  K V  Q+ V P +Q
Sbjct: 383 FEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPEQQ 438

[Close]

BLAST of Lsi06G006330 vs. TrEMBL
Match: A0A068UE71_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00023876001 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.1e-09
Identity = 45/106 (42.45%), Postives = 67/106 (63.21%), Query Frame = 1


Query: 7   EIQRQYQELCLQLCTESGKALKSIAMAIRDIIIS-PALAKSHIKIAKEKAEELMSFLTTS 66
           EI+ ++QE C  + +ESG ALK++A AIR +  S  A+AKSHI IAK  A+ L S L T+
Sbjct: 323 EIREKFQESCQTMSSESGHALKNMARAIRTMTSSYVAVAKSHIAIAKSAADNLKSLLKTN 382

Query: 67  HFNGD--MKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTV 110
            +     ++++   T+  LLI+ +S   KIADS+ +  SLAR K +
Sbjct: 383 SWQQTDLLEIIPVITVASLLIEVVSSTVKIADSVHQLASLARFKKI 428

[Close]

BLAST of Lsi06G006330 vs. TrEMBL
Match: W9SDG7_9ROSA (Aluminum-activated malate transporter 2 OS=Morus notabilis GN=L484_001463 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.1e-09
Identity = 52/134 (38.81%), Postives = 80/134 (59.70%), Query Frame = 1


Query: 6   LEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTS 65
           +EI+R+ QE C ++CTESGKALK +A A++  I S + + +HI  +K  AE L S L   
Sbjct: 316 IEIRRKIQEPCTKICTESGKALKELAAAMKKRIRSSSFS-AHITNSKNAAENLKSLLKRY 375

Query: 66  HF---NGD-MKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVLKQAGVTPTEQK 125
            +   +GD + +V    + L+LID + C++ IA+++ E  S+AR KT  + A V   EQ 
Sbjct: 376 EWQDHDGDLLDIVPGAAVALVLIDVVKCIDDIAETVVELSSVARFKT--EDATVVALEQP 435

Query: 126 PH-HNITITQIQIG 135
           P   +  I Q Q+G
Sbjct: 436 PSLLHRAIVQPQLG 446

[Close]

BLAST of Lsi06G006330 vs. TrEMBL
Match: A0A0A0M0C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630860 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 8.9e-09
Identity = 45/108 (41.67%), Postives = 66/108 (61.11%), Query Frame = 1


Query: 3   PLHLEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFL 62
           PLH  I+ Q +E C ++ TESGKALK +A +IR + + P L   HI+ +K  A++L + L
Sbjct: 314 PLH--IRDQLKESCSKMSTESGKALKDLASSIRTMTL-PRLPNPHIEKSKAAAKDLKAAL 373

Query: 63  TTSHFNGD---MKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLK 108
                N     +++V   T+  LLID +SC+EKIA+S+ E  SLA  K
Sbjct: 374 KIRPCNSSIDLLEIVPMATVASLLIDSISCIEKIAESVGELASLANFK 418

[Close]

BLAST of Lsi06G006330 vs. TAIR10
Match: AT3G11680.1 (AT3G11680.1 Aluminium activated malate transporter family protein)

HSP 1 Score: 48.5 bits (114), Expect = 3.7e-06
Identity = 32/107 (29.91%), Postives = 59/107 (55.14%), Query Frame = 1


Query: 7   EIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTSH 66
           E + + QE    +  E G+ALK+IA +I+ +    A   +HI  +K+  + L   L +S+
Sbjct: 316 EFESKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSKKAIKNLKIALKSSY 375

Query: 67  ---FNGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVL 111
              +   ++++   T+  +LI+ ++CVEKI +++ EF  LA  K  L
Sbjct: 376 PETYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETL 422

[Close]

BLAST of Lsi06G006330 vs. NCBI nr
Match: gi|449442737|ref|XP_004139137.1| (PREDICTED: aluminum-activated malate transporter 2 [Cucumis sativus])

HSP 1 Score: 175.6 bits (444), Expect = 5.7e-41
Identity = 101/132 (76.52%), Postives = 106/132 (80.30%), Query Frame = 1


Query: 6   LEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTS 65
           LEIQRQYQE CLQLC ESGKAL SIAMAIRDII  PA+AKSH + AKEKAE LMS L +S
Sbjct: 316 LEIQRQYQEHCLQLCIESGKALNSIAMAIRDII-PPAMAKSHTEKAKEKAEALMSLLKSS 375

Query: 66  HFNGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTV--LKQAGVTPTEQKPH 125
           HFNGDMKMVSTTTLI LLIDCLSCVEKI DS+ + VSLAR KT    KQAGV  TEQK  
Sbjct: 376 HFNGDMKMVSTTTLIYLLIDCLSCVEKIVDSVHDLVSLARPKTTHPPKQAGVMSTEQKAP 435

Query: 126 HNITITQIQIGE 136
           HNI IT IQI E
Sbjct: 436 HNIIITHIQIAE 446

[Close]

BLAST of Lsi06G006330 vs. NCBI nr
Match: gi|700211483|gb|KGN66579.1| (hypothetical protein Csa_1G630340 [Cucumis sativus])

HSP 1 Score: 175.6 bits (444), Expect = 5.7e-41
Identity = 101/132 (76.52%), Postives = 106/132 (80.30%), Query Frame = 1


Query: 6   LEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTS 65
           LEIQRQYQE CLQLC ESGKAL SIAMAIRDII  PA+AKSH + AKEKAE LMS L +S
Sbjct: 51  LEIQRQYQEHCLQLCIESGKALNSIAMAIRDII-PPAMAKSHTEKAKEKAEALMSLLKSS 110

Query: 66  HFNGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTV--LKQAGVTPTEQKPH 125
           HFNGDMKMVSTTTLI LLIDCLSCVEKI DS+ + VSLAR KT    KQAGV  TEQK  
Sbjct: 111 HFNGDMKMVSTTTLIYLLIDCLSCVEKIVDSVHDLVSLARPKTTHPPKQAGVMSTEQKAP 170

Query: 126 HNITITQIQIGE 136
           HNI IT IQI E
Sbjct: 171 HNIIITHIQIAE 181

[Close]

BLAST of Lsi06G006330 vs. NCBI nr
Match: gi|449442739|ref|XP_004139138.1| (PREDICTED: aluminum-activated malate transporter 2-like [Cucumis sativus])

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-13
Identity = 47/117 (40.17%), Postives = 73/117 (62.39%), Query Frame = 1


Query: 7   EIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTSH 66
           +I  Q++E C+++C+ES + L+ +A+A+R +++ P  AKSHI+ AK  AE L S L    
Sbjct: 323 QIHGQFKEECMKICSESSRGLRELALALRKMVL-PLTAKSHIEKAKIAAENLKSHLEEWR 382

Query: 67  F---NGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVLKQAGVTPTEQ 121
           F   N  M++V   +L  LL D + C+EKI DS++E  S+A  K V  Q+ V P +Q
Sbjct: 383 FEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPEQQ 438

[Close]

BLAST of Lsi06G006330 vs. NCBI nr
Match: gi|659098790|ref|XP_008450297.1| (PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo])

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-12
Identity = 46/117 (39.32%), Postives = 73/117 (62.39%), Query Frame = 1


Query: 7   EIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLTTSH 66
           +I   ++E C+++C+ES + L+ +A+A+R +++ P  AKSHI+ AK  AE+L S L    
Sbjct: 322 QIHGPFKEECMKICSESSRGLRELALALRKMVL-PLTAKSHIEKAKVAAEDLKSHLEEWR 381

Query: 67  F---NGDMKMVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVLKQAGVTPTEQ 121
           F   N  M++V   +L  LL D + C+EKI DS++E  S+A  K V  Q+ V P +Q
Sbjct: 382 FEEVNNAMEIVQVVSLASLLFDTICCIEKIVDSVQELASMAGFKAVEVQSSVAPEQQ 437

[Close]

BLAST of Lsi06G006330 vs. NCBI nr
Match: gi|719975279|ref|XP_010247137.1| (PREDICTED: aluminum-activated malate transporter 8 isoform X2 [Nelumbo nucifera])

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-09
Identity = 46/130 (35.38%), Postives = 78/130 (60.00%), Query Frame = 1


Query: 4   LHLEIQRQYQELCLQLCTESGKALKSIAMAIRDIIISPALAKSHIKIAKEKAEELMSFLT 63
           ++ EIQR+ Q++C+ + +ESGKAL  ++ AI+  +  P+LA  H++  K  A +L + L 
Sbjct: 341 INTEIQRKIQDVCVTMSSESGKALMELSAAIK-AMTHPSLADLHVENCKNAARDLKTTLK 400

Query: 64  TSHF-NGDMK-MVSTTTLILLLIDCLSCVEKIADSIREFVSLARLKTVLKQAGVTPTEQK 123
           T+   N D+  ++ + T+  LL+D + C EKIA+S+ E   LA  KTV  +  VTP + +
Sbjct: 401 TALLENSDLLGVIPSATVASLLVDVVVCTEKIAESVHELAKLAHFKTV--EPTVTPEKSQ 460

Query: 124 PHHNITITQI 132
             H  T+  +
Sbjct: 461 LLHRGTVNPL 467

The following BLAST results are available for this feature:

BLAST of Lsi06G006330 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi06G006330 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LZX9_CUCSA	4.0e-41	76.52	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630340 PE=4 SV=1	[more]
A0A0A0M301_CUCSA	3.5e-13	40.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630850 PE=4 SV=1	[more]
A0A068UE71_COFCA	3.1e-09	42.45	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00023876001 PE=4 SV=1	[more]
W9SDG7_9ROSA	3.1e-09	38.81	Aluminum-activated malate transporter 2 OS=Morus notabilis GN=L484_001463 PE=4 S...	[more]
A0A0A0M0C7_CUCSA	8.9e-09	41.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G630860 PE=4 SV=1	[more]

BLAST of Lsi06G006330 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G11680.1	3.7e-06	29.91	Aluminium activated malate transporter family protein	[more]

BLAST of Lsi06G006330 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442737\|ref\|XP_004139137.1\|	5.7e-41	76.52	PREDICTED: aluminum-activated malate transporter 2 [Cucumis sativus]	[more]
gi\|700211483\|gb\|KGN66579.1\|	5.7e-41	76.52	hypothetical protein Csa_1G630340 [Cucumis sativus]	[more]
gi\|449442739\|ref\|XP_004139138.1\|	5.0e-13	40.17	PREDICTED: aluminum-activated malate transporter 2-like [Cucumis sativus]	[more]
gi\|659098790\|ref\|XP_008450297.1\|	1.1e-12	39.32	PREDICTED: aluminum-activated malate transporter 2-like [Cucumis melo]	[more]
gi\|719975279\|ref\|XP_010247137.1\|	2.0e-09	35.38	PREDICTED: aluminum-activated malate transporter 8 isoform X2 [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0015743	malate transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G006330.1	Lsi06G006330.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31086	FAMILY NOT NAMED	coord: 6..133 score: 2.3
None	No IPR available	PANTHER	PTHR31086:SF8	SUBFAMILY NOT NAMED	coord: 6..133 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G006330	ClCG03G012080	Watermelon (Charleston Gray)	lsiwcgB364
Lsi06G006330	MELO3C015075.2	Melon (DHL92) v3.6.1	lsimedB428
Lsi06G006330	Bhi02G001197	Wax gourd	lsiwgoB509
Lsi06G006330	Carg22686	Silver-seed gourd	carlsiB486
Lsi06G006330	CsaV3_1G043720	Cucumber (Chinese Long) v3	cuclsiB087

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G006330	Cucumber (Gy14) v1	cgylsiB573
Lsi06G006330	Cucumber (Gy14) v1	cgylsiB574
Lsi06G006330	Cucurbita maxima (Rimu)	cmalsiB067
Lsi06G006330	Cucurbita maxima (Rimu)	cmalsiB073
Lsi06G006330	Cucurbita maxima (Rimu)	cmalsiB128
Lsi06G006330	Cucurbita moschata (Rifu)	cmolsiB054
Lsi06G006330	Cucurbita moschata (Rifu)	cmolsiB059
Lsi06G006330	Cucurbita moschata (Rifu)	cmolsiB118
Lsi06G006330	Cucurbita moschata (Rifu)	cmolsiB135
Lsi06G006330	Cucurbita pepo (Zucchini)	cpelsiB017
Lsi06G006330	Cucurbita pepo (Zucchini)	cpelsiB299
Lsi06G006330	Cucurbita pepo (Zucchini)	cpelsiB309
Lsi06G006330	Cucurbita pepo (Zucchini)	cpelsiB562
Lsi06G006330	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G006330	Wild cucumber (PI 183967)	cpilsiB077
Lsi06G006330	Cucumber (Chinese Long) v2	culsiB058
Lsi06G006330	Cucumber (Chinese Long) v2	culsiB077
Lsi06G006330	Melon (DHL92) v3.5.1	lsimeB396
Lsi06G006330	Melon (DHL92) v3.5.1	lsimeB406
Lsi06G006330	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G006330	Cucumber (Gy14) v2	cgyblsiB072
Lsi06G006330	Melon (DHL92) v3.6.1	lsimedB418
Lsi06G006330	Silver-seed gourd	carlsiB485
Lsi06G006330	Silver-seed gourd	carlsiB885
Lsi06G006330	Silver-seed gourd	carlsiB886
Lsi06G006330	Cucumber (Chinese Long) v3	cuclsiB068
Lsi06G006330	Watermelon (97103) v2	lsiwmbB365