Lsi06G001230 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi06G001230
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPentatricopeptide repeat-containing protein
Locationchr06 : 1200861 .. 1202720 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCACTTCTTGCCCTTCATCCCACTGCATCCCTTCCCAATTCACCAAAACTCCCCACATCACCCATCTTCCACGCCCTCAATTCATGCTCATCAATGGCTGAGCTCAAGCAATTTCACTCCCAAATCATCCGTTTTGGTCTCTCTACTGACAACGATGCCATCGGCCGTCTCATCAAATTCTGTGCTGTTTCCAAGTACGGAGATCTTCACTATGCTCTTCTTTTATTCAACTCAATCCCTTACCCAGATGCTTTTATTTACAATACTTTAATTAGAGCTTACTTACAGTTCCAATCTCCTACAGCTTCCTTACTTTTGTATTTGCAAATGCTTCATAACTCTGTCCTTCCCAATAAATTCACATTCCCTTCTTTGATTCGTGCTTGCTGTATTCATAATGCTGTTAAGGAAGGGAAACAAATTCATGCCCATGTTCTTAAATTTGGTTTCGGAACAGATAGATTTTCGAACAACAATTTAATTCATATGTATGCTAATTTTCAATCCTTGGAAGAAGCTACAAGGGTGTTTGATTCTATTGAGTTACCTGATGTTGTAGCTTGGACTACTTTGCTTACTGGGTATGCTCAATTTGGATTTGTAGATGAAGCTTTACGAGTTTTCGAGTCGATGCCTGAGCGTAACTCTGCTTCTTGGAATGCTATGATTTCTTGTTTTGTTCAAAACAATAGATTTCATGAGGCATTTGGTTTGTTTAATAGGATGAGATTAGAGAAGGTTGTTTTGGAGAAATATGTAGCTGCTAGTATGTTATCAGCTTGTACAGGATTAGGAGCACTTGAACAAGGGAAATGGATACATAGATACATTAAGAGAAATGGGATTGAATTGGATTCAAAACTTGCAACTACATTGATTGATATGTATTGTAAATGTGGTTGTTTGGATTGTGCTTTCCAAGTGTTTACTCATTTACCTCAAAAGGGAATTTCTTCATGGAATTGTATGATTGGAGGGATGGCTATGCATGGGAAAGGAGAGGCAGCTATTGAGCTTTTTAAAGAAATGGAGACCAAACTGGTGAAACCAGACAACATAACTTTCCTTAATGTACTAAGTGCTTGTGCTCACTCTGGGTTAGTCGAAAAGGGGCGATACTATTTCTCTCGTTTTACTCAAGTTTACAGTATTGAACCTGTAACCGAGCATTATGGATGCATGGTTGATTTGTATGGACGAGCCGGGATGTTGGAGGAAGCAATGAAAGTCATAGATGAGATGCCTATGAGTCCTGATGTAGGTGTTTTAGGTGCCTTTGTTGGAGCTTGTAAAATTCATGGGAATATAGAGTTGGGAGAGGAAATAGGGAAGAGAGTAATAGAATTAGAACCTACGAATAGCGGGCGTTACGTTCTACTGGGAAATCTATATGCCGAGGCGGGTAGATGGGAACGTGTTGCAGAAGTAAGAAAGTTAATGAATGATAGAGAAGTGAAGAAGGCTGCTGGAGTTTCCATGATTGAATTGGAAGGTGTTGTGTATGAATTTATTGCAGGAGGAAGAGTTCATCCTGAAGCAAAGGAAATTTATGCTAAACTTAATGAGATGTTAGAATGTATAAGAACTGAAGGATATGTAGCAGAGAATGGAATTGAGGAGGAGAAGGATAATCCTGTTTATTACCATAGTGAGAAACTGGCAATTGCTTTTGGGTTGCTGAAAACTAAAGCAGGGGAAATGCTTAGAATTACTAAGAATCTGAGGGTTTGTAAGGATTGTCACCAAGCTTTGAAGCTTGTTTCCAAGGTTTTTCAAAGAAAAATCATTGTAAGAGACAGAAATCGTTTCCATCATTTTGCTAATGGTGAGTGTTCTTGTAATGATTATTGGTAA

mRNA sequence

ATGGCTTCACTTCTTGCCCTTCATCCCACTGCATCCCTTCCCAATTCACCAAAACTCCCCACATCACCCATCTTCCACGCCCTCAATTCATGCTCATCAATGGCTGAGCTCAAGCAATTTCACTCCCAAATCATCCGTTTTGGTCTCTCTACTGACAACGATGCCATCGGCCGTCTCATCAAATTCTGTGCTGTTTCCAAGTACGGAGATCTTCACTATGCTCTTCTTTTATTCAACTCAATCCCTTACCCAGATGCTTTTATTTACAATACTTTAATTAGAGCTTACTTACAGTTCCAATCTCCTACAGCTTCCTTACTTTTGTATTTGCAAATGCTTCATAACTCTGTCCTTCCCAATAAATTCACATTCCCTTCTTTGATTCGTGCTTGCTGTATTCATAATGCTGTTAAGGAAGGGAAACAAATTCATGCCCATGTTCTTAAATTTGGTTTCGGAACAGATAGATTTTCGAACAACAATTTAATTCATATGTATGCTAATTTTCAATCCTTGGAAGAAGCTACAAGGGTGTTTGATTCTATTGAGTTACCTGATGTTGTAGCTTGGACTACTTTGCTTACTGGGTATGCTCAATTTGGATTTGTAGATGAAGCTTTACGAGTTTTCGAGTCGATGCCTGAGCGTAACTCTGCTTCTTGGAATGCTATGATTTCTTGTTTTGTTCAAAACAATAGATTTCATGAGGCATTTGGTTTGTTTAATAGGATGAGATTAGAGAAGGTTGTTTTGGAGAAATATGTAGCTGCTAGTATGTTATCAGCTTGTACAGGATTAGGAGCACTTGAACAAGGGAAATGGATACATAGATACATTAAGAGAAATGGGATTGAATTGGATTCAAAACTTGCAACTACATTGATTGATATGTATTGTAAATGTGGTTGTTTGGATTGTGCTTTCCAAGTGTTTACTCATTTACCTCAAAAGGGAATTTCTTCATGGAATTGTATGATTGGAGGGATGGCTATGCATGGGAAAGGAGAGGCAGCTATTGAGCTTTTTAAAGAAATGGAGACCAAACTGGTGAAACCAGACAACATAACTTTCCTTAATGTACTAAGTGCTTGTGCTCACTCTGGGTTAGTCGAAAAGGGGCGATACTATTTCTCTCGTTTTACTCAAGTTTACAGTATTGAACCTGTAACCGAGCATTATGGATGCATGGTTGATTTGTATGGACGAGCCGGGATGTTGGAGGAAGCAATGAAAGTCATAGATGAGATGCCTATGAGTCCTGATGTAGGTGTTTTAGGTGCCTTTGTTGGAGCTTGTAAAATTCATGGGAATATAGAGTTGGGAGAGGAAATAGGGAAGAGAGTAATAGAATTAGAACCTACGAATAGCGGGCGTTACGTTCTACTGGGAAATCTATATGCCGAGGCGGGTAGATGGGAACGTGTTGCAGAAGTAAGAAAGTTAATGAATGATAGAGAAGTGAAGAAGGCTGCTGGAGTTTCCATGATTGAATTGGAAGGTGTTGTGTATGAATTTATTGCAGGAGGAAGAGTTCATCCTGAAGCAAAGGAAATTTATGCTAAACTTAATGAGATGTTAGAATGTATAAGAACTGAAGGATATGTAGCAGAGAATGGAATTGAGGAGGAGAAGGATAATCCTGTTTATTACCATAGTGAGAAACTGGCAATTGCTTTTGGGTTGCTGAAAACTAAAGCAGGGGAAATGCTTAGAATTACTAAGAATCTGAGGGTTTGTAAGGATTGTCACCAAGCTTTGAAGCTTGTTTCCAAGGTTTTTCAAAGAAAAATCATTGTAAGAGACAGAAATCGTTTCCATCATTTTGCTAATGGTGAGTGTTCTTGTAATGATTATTGGTAA

Coding sequence (CDS)

ATGGCTTCACTTCTTGCCCTTCATCCCACTGCATCCCTTCCCAATTCACCAAAACTCCCCACATCACCCATCTTCCACGCCCTCAATTCATGCTCATCAATGGCTGAGCTCAAGCAATTTCACTCCCAAATCATCCGTTTTGGTCTCTCTACTGACAACGATGCCATCGGCCGTCTCATCAAATTCTGTGCTGTTTCCAAGTACGGAGATCTTCACTATGCTCTTCTTTTATTCAACTCAATCCCTTACCCAGATGCTTTTATTTACAATACTTTAATTAGAGCTTACTTACAGTTCCAATCTCCTACAGCTTCCTTACTTTTGTATTTGCAAATGCTTCATAACTCTGTCCTTCCCAATAAATTCACATTCCCTTCTTTGATTCGTGCTTGCTGTATTCATAATGCTGTTAAGGAAGGGAAACAAATTCATGCCCATGTTCTTAAATTTGGTTTCGGAACAGATAGATTTTCGAACAACAATTTAATTCATATGTATGCTAATTTTCAATCCTTGGAAGAAGCTACAAGGGTGTTTGATTCTATTGAGTTACCTGATGTTGTAGCTTGGACTACTTTGCTTACTGGGTATGCTCAATTTGGATTTGTAGATGAAGCTTTACGAGTTTTCGAGTCGATGCCTGAGCGTAACTCTGCTTCTTGGAATGCTATGATTTCTTGTTTTGTTCAAAACAATAGATTTCATGAGGCATTTGGTTTGTTTAATAGGATGAGATTAGAGAAGGTTGTTTTGGAGAAATATGTAGCTGCTAGTATGTTATCAGCTTGTACAGGATTAGGAGCACTTGAACAAGGGAAATGGATACATAGATACATTAAGAGAAATGGGATTGAATTGGATTCAAAACTTGCAACTACATTGATTGATATGTATTGTAAATGTGGTTGTTTGGATTGTGCTTTCCAAGTGTTTACTCATTTACCTCAAAAGGGAATTTCTTCATGGAATTGTATGATTGGAGGGATGGCTATGCATGGGAAAGGAGAGGCAGCTATTGAGCTTTTTAAAGAAATGGAGACCAAACTGGTGAAACCAGACAACATAACTTTCCTTAATGTACTAAGTGCTTGTGCTCACTCTGGGTTAGTCGAAAAGGGGCGATACTATTTCTCTCGTTTTACTCAAGTTTACAGTATTGAACCTGTAACCGAGCATTATGGATGCATGGTTGATTTGTATGGACGAGCCGGGATGTTGGAGGAAGCAATGAAAGTCATAGATGAGATGCCTATGAGTCCTGATGTAGGTGTTTTAGGTGCCTTTGTTGGAGCTTGTAAAATTCATGGGAATATAGAGTTGGGAGAGGAAATAGGGAAGAGAGTAATAGAATTAGAACCTACGAATAGCGGGCGTTACGTTCTACTGGGAAATCTATATGCCGAGGCGGGTAGATGGGAACGTGTTGCAGAAGTAAGAAAGTTAATGAATGATAGAGAAGTGAAGAAGGCTGCTGGAGTTTCCATGATTGAATTGGAAGGTGTTGTGTATGAATTTATTGCAGGAGGAAGAGTTCATCCTGAAGCAAAGGAAATTTATGCTAAACTTAATGAGATGTTAGAATGTATAAGAACTGAAGGATATGTAGCAGAGAATGGAATTGAGGAGGAGAAGGATAATCCTGTTTATTACCATAGTGAGAAACTGGCAATTGCTTTTGGGTTGCTGAAAACTAAAGCAGGGGAAATGCTTAGAATTACTAAGAATCTGAGGGTTTGTAAGGATTGTCACCAAGCTTTGAAGCTTGTTTCCAAGGTTTTTCAAAGAAAAATCATTGTAAGAGACAGAAATCGTTTCCATCATTTTGCTAATGGTGAGTGTTCTTGTAATGATTATTGGTAA

Protein sequence

MASLLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGIEEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFANGECSCNDYW
BLAST of Lsi06G001230 vs. Swiss-Prot
Match: PP449_ARATH (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 5.5e-155
Identity = 273/602 (45.35%), Postives = 389/602 (64.62%), Query Frame = 1

Query: 28  LNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGD-LHYALLLFNSIPYPDA 87
           L  CS   ELKQ H+++++ GL  D+ AI + + FC  S   D L YA ++F+    PD 
Sbjct: 21  LQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQIVFDGFDRPDT 80

Query: 88  FIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAVKEGKQIHAH 147
           F++N +IR +     P  SLLLY +ML +S   N +TFPSL++AC   +A +E  QIHA 
Sbjct: 81  FLWNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQ 140

Query: 148 VLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGYAQFGFVDEA 207
           + K G+  D ++ N+LI+ YA   + + A  +FD I  PD V+W +++ GY + G +D A
Sbjct: 141 ITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIA 200

Query: 208 LRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEKVVLEKYVAASMLSACTGL 267
           L +F  M E+N+ SW  MIS +VQ +   EA  LF+ M+   V  +    A+ LSAC  L
Sbjct: 201 LTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQL 260

Query: 268 GALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQKGISSWNCMI 327
           GALEQGKWIH Y+ +  I +DS L   LIDMY KCG ++ A +VF ++ +K + +W  +I
Sbjct: 261 GALEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALI 320

Query: 328 GGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEKGRYYFSRFTQVYSI 387
            G A HG G  AI  F EM+   +KP+ ITF  VL+AC+++GLVE+G+  F    + Y++
Sbjct: 321 SGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSMERDYNL 380

Query: 388 EPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHGNIELGEEIGK 447
           +P  EHYGC+VDL GRAG+L+EA + I EMP+ P+  + GA + AC+IH NIELGEEIG+
Sbjct: 381 KPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGE 440

Query: 448 RVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMIELEGVVYEFI 507
            +I ++P + GRYV   N++A   +W++ AE R+LM ++ V K  G S I LEG  +EF+
Sbjct: 441 ILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTISLEGTTHEFL 500

Query: 508 AGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGIEE---------EKDNPVYYHSEKLAI 567
           AG R HPE ++I +K   M   +   GYV E  +EE         E++  V+ HSEKLAI
Sbjct: 501 AGDRSHPEIEKIQSKWRIMRRKLEENGYVPE--LEEMLLDLVDDDEREAIVHQHSEKLAI 560

Query: 568 AFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFANGECSCND 620
            +GL+KTK G ++RI KNLRVCKDCH+  KL+SK+++R I++RDR RFHHF +G+CSC D
Sbjct: 561 TYGLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRDIVMRDRTRFHHFRDGKCSCGD 620

BLAST of Lsi06G001230 vs. Swiss-Prot
Match: PP425_ARATH (Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana GN=PCMP-H38 PE=2 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 4.2e-147
Identity = 256/630 (40.63%), Postives = 390/630 (61.90%), Query Frame = 1

Query: 15  NSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSK--YGDLH 74
           +SP    S +F  +N+C ++ +L Q H+  I+ G   D  A   +++FCA S   + DL 
Sbjct: 17  SSPASHPSSLFPQINNCRTIRDLSQIHAVFIKSGQMRDTLAAAEILRFCATSDLHHRDLD 76

Query: 75  YALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASL---LLYLQMLHNSVLPNKFTFPSLIR 134
           YA  +FN +P  + F +NT+IR + +     A +   L Y  M    V PN+FTFPS+++
Sbjct: 77  YAHKIFNQMPQRNCFSWNTIIRGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLK 136

Query: 135 ACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVF-------DSI 194
           AC     ++EGKQIH   LK+GFG D F  +NL+ MY     +++A  +F       D +
Sbjct: 137 ACAKTGKIQEGKQIHGLALKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMV 196

Query: 195 ELPD-------VVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFH 254
            + D       +V W  ++ GY + G    A  +F+ M +R+  SWN MIS +  N  F 
Sbjct: 197 VMTDRRKRDGEIVLWNVMIDGYMRLGDCKAARMLFDKMRQRSVVSWNTMISGYSLNGFFK 256

Query: 255 EAFGLFNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLI 314
           +A  +F  M+   +        S+L A + LG+LE G+W+H Y + +GI +D  L + LI
Sbjct: 257 DAVEVFREMKKGDIRPNYVTLVSVLPAISRLGSLELGEWLHLYAEDSGIRIDDVLGSALI 316

Query: 315 DMYCKCGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNI 374
           DMY KCG ++ A  VF  LP++ + +W+ MI G A+HG+   AI+ F +M    V+P ++
Sbjct: 317 DMYSKCGIIEKAIHVFERLPRENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDV 376

Query: 375 TFLNVLSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDE 434
            ++N+L+AC+H GLVE+GR YFS+   V  +EP  EHYGCMVDL GR+G+L+EA + I  
Sbjct: 377 AYINLLTACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILN 436

Query: 435 MPMSPDVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERV 494
           MP+ PD  +  A +GAC++ GN+E+G+ +   ++++ P +SG YV L N+YA  G W  V
Sbjct: 437 MPIKPDDVIWKALLGACRMQGNVEMGKRVANILMDMVPHDSGAYVALSNMYASQGNWSEV 496

Query: 495 AEVRKLMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGY- 554
           +E+R  M +++++K  G S+I+++GV++EF+     HP+AKEI + L E+ + +R  GY 
Sbjct: 497 SEMRLRMKEKDIRKDPGCSLIDIDGVLHEFVVEDDSHPKAKEINSMLVEISDKLRLAGYR 556

Query: 555 -----VAENGIEEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLV 614
                V  N  EE+K+N ++YHSEK+A AFGL+ T  G+ +RI KNLR+C+DCH ++KL+
Sbjct: 557 PITTQVLLNLEEEDKENVLHYHSEKIATAFGLISTSPGKPIRIVKNLRICEDCHSSIKLI 616

Query: 615 SKVFQRKIIVRDRNRFHHFANGECSCNDYW 620
           SKV++RKI VRDR RFHHF +G CSC DYW
Sbjct: 617 SKVYKRKITVRDRKRFHHFQDGSCSCMDYW 646

BLAST of Lsi06G001230 vs. Swiss-Prot
Match: PP367_ARATH (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 2.0e-141
Identity = 254/609 (41.71%), Postives = 376/609 (61.74%), Query Frame = 1

Query: 23  PIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGD-----LHYALLL 82
           P    L SCSS ++LK  H  ++R  L +D     RL+  C      +     L YA  +
Sbjct: 14  PKLALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGI 73

Query: 83  FNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAV 142
           F+ I  P+ F++N LIR +     P+ +   Y QML + + P+  TFP LI+A      V
Sbjct: 74  FSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECV 133

Query: 143 KEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGY 202
             G+Q H+ +++FGF  D +  N+L+HMYAN   +  A R+F  +   DVV+WT+++ GY
Sbjct: 134 LVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGY 193

Query: 203 AQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEKVVLEKYVAA 262
            + G V+ A  +F+ MP RN  +W+ MI+ + +NN F +A  LF  M+ E VV  + V  
Sbjct: 194 CKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMV 253

Query: 263 SMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQK 322
           S++S+C  LGALE G+  + Y+ ++ + ++  L T L+DM+ +CG ++ A  VF  LP+ 
Sbjct: 254 SVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGLPET 313

Query: 323 GISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEKGRYYF 382
              SW+ +I G+A+HG    A+  F +M +    P ++TF  VLSAC+H GLVEKG   +
Sbjct: 314 DSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIY 373

Query: 383 SRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHGN 442
               + + IEP  EHYGC+VD+ GRAG L EA   I +M + P+  +LGA +GACKI+ N
Sbjct: 374 ENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKN 433

Query: 443 IELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMIE 502
            E+ E +G  +I+++P +SG YVLL N+YA AG+W+++  +R +M ++ VKK  G S+IE
Sbjct: 434 TEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIE 493

Query: 503 LEGVVYEFIAG-GRVHPEAKEIYAKLNEMLECIRTEGYVAENG------IEEEKDNPVYY 562
           ++G + +F  G  + HPE  +I  K  E+L  IR  GY    G       EEEK++ ++ 
Sbjct: 494 IDGKINKFTMGDDQKHPEMGKIRRKWEEILGKIRLIGYKGNTGDAFFDVDEEEKESSIHM 553

Query: 563 HSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFAN 620
           HSEKLAIA+G++KTK G  +RI KNLRVC+DCH   KL+S+V+ R++IVRDRNRFHHF N
Sbjct: 554 HSEKLAIAYGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFRN 613

BLAST of Lsi06G001230 vs. Swiss-Prot
Match: PP175_ARATH (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 7.4e-136
Identity = 246/614 (40.07%), Postives = 381/614 (62.05%), Query Frame = 1

Query: 14  PNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGDLHY 73
           PN    P   +  A    SS++  +  H   ++  + +D      LI  C  S  GDL  
Sbjct: 129 PNKYTFPF--LIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIH-CYFS-CGDLDS 188

Query: 74  ALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCI 133
           A  +F +I   D   +N++I  ++Q  SP  +L L+ +M    V  +  T   ++ AC  
Sbjct: 189 ACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACAK 248

Query: 134 HNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTL 193
              ++ G+Q+ +++ +     +    N ++ MY    S+E+A R+FD++E  D V WTT+
Sbjct: 249 IRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTM 308

Query: 194 LTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEK-VVLE 253
           L GYA     + A  V  SMP+++  +WNA+IS + QN + +EA  +F+ ++L+K + L 
Sbjct: 309 LDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNMKLN 368

Query: 254 KYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFT 313
           +    S LSAC  +GALE G+WIH YIK++GI ++  + + LI MY KCG L+ + +VF 
Sbjct: 369 QITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSREVFN 428

Query: 314 HLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEK 373
            + ++ +  W+ MIGG+AMHG G  A+++F +M+   VKP+ +TF NV  AC+H+GLV++
Sbjct: 429 SVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDE 488

Query: 374 GRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGAC 433
               F +    Y I P  +HY C+VD+ GR+G LE+A+K I+ MP+ P   V GA +GAC
Sbjct: 489 AESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGAC 548

Query: 434 KIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAG 493
           KIH N+ L E    R++ELEP N G +VLL N+YA+ G+WE V+E+RK M    +KK  G
Sbjct: 549 KIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKKEPG 608

Query: 494 VSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENG-----IEEE--KD 553
            S IE++G+++EF++G   HP ++++Y KL+E++E +++ GY  E       IEEE  K+
Sbjct: 609 CSSIEIDGMIHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEISQVLQIIEEEEMKE 668

Query: 554 NPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRF 613
             +  HSEKLAI +GL+ T+A +++R+ KNLRVC DCH   KL+S+++ R+IIVRDR RF
Sbjct: 669 QSLNLHSEKLAICYGLISTEAPKVIRVIKNLRVCGDCHSVAKLISQLYDREIIVRDRYRF 728

Query: 614 HHFANGECSCNDYW 620
           HHF NG+CSCND+W
Sbjct: 729 HHFRNGQCSCNDFW 738

BLAST of Lsi06G001230 vs. Swiss-Prot
Match: PP354_ARATH (Pentatricopeptide repeat-containing protein ELI1, chloroplastic OS=Arabidopsis thaliana GN=ELI1 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 6.3e-135
Identity = 256/609 (42.04%), Postives = 366/609 (60.10%), Query Frame = 1

Query: 20  PTSPIFHALNSCSSMAELKQFHSQIIRFGLSTD-NDAIGRLIKFCAVSKYGDLHYALLLF 79
           P   +   ++   S+ E+ Q H+ I+R  L       +  L    A + +G + ++L LF
Sbjct: 28  PPEKLAVLIDKSQSVDEVLQIHAAILRHNLLLHPRYPVLNLKLHRAYASHGKIRHSLALF 87

Query: 80  NSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAVK 139
           +    PD F++   I           + LLY+Q+L + + PN+FTF SL+++C    + K
Sbjct: 88  HQTIDPDLFLFTAAINTASINGLKDQAFLLYVQLLSSEINPNEFTFSSLLKSC----STK 147

Query: 140 EGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGYA 199
            GK IH HVLKFG G D +    L+ +YA    +  A +VFD +    +V+ T ++T YA
Sbjct: 148 SGKLIHTHVLKFGLGIDPYVATGLVDVYAKGGDVVSAQKVFDRMPERSLVSSTAMITCYA 207

Query: 200 QFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLE-KVVLEKYVAA 259
           + G V+ A  +F+SM ER+  SWN MI  + Q+   ++A  LF ++  E K   ++    
Sbjct: 208 KQGNVEAARALFDSMCERDIVSWNVMIDGYAQHGFPNDALMLFQKLLAEGKPKPDEITVV 267

Query: 260 SMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQK 319
           + LSAC+ +GALE G+WIH ++K + I L+ K+ T LIDMY KCG L+ A  VF   P+K
Sbjct: 268 AALSACSQIGALETGRWIHVFVKSSRIRLNVKVCTGLIDMYSKCGSLEEAVLVFNDTPRK 327

Query: 320 GISSWNCMIGGMAMHGKGEAAIELFKEMETKL-VKPDNITFLNVLSACAHSGLVEKGRYY 379
            I +WN MI G AMHG  + A+ LF EM+    ++P +ITF+  L ACAH+GLV +G   
Sbjct: 328 DIVAWNAMIAGYAMHGYSQDALRLFNEMQGITGLQPTDITFIGTLQACAHAGLVNEGIRI 387

Query: 380 FSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHG 439
           F    Q Y I+P  EHYGC+V L GRAG L+ A + I  M M  D  +  + +G+CK+HG
Sbjct: 388 FESMGQEYGIKPKIEHYGCLVSLLGRAGQLKRAYETIKNMNMDADSVLWSSVLGSCKLHG 447

Query: 440 NIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMI 499
           +  LG+EI + +I L   NSG YVLL N+YA  G +E VA+VR LM ++ + K  G+S I
Sbjct: 448 DFVLGKEIAEYLIGLNIKNSGIYVLLSNIYASVGDYEGVAKVRNLMKEKGIVKEPGISTI 507

Query: 500 ELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGI------EEEKDNPVYY 559
           E+E  V+EF AG R H ++KEIY  L ++ E I++ GYV           E EK+  +  
Sbjct: 508 EIENKVHEFRAGDREHSKSKEIYTMLRKISERIKSHGYVPNTNTVLQDLEETEKEQSLQV 567

Query: 560 HSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFAN 619
           HSE+LAIA+GL+ TK G  L+I KNLRVC DCH   KL+SK+  RKI++RDRNRFHHF +
Sbjct: 568 HSERLAIAYGLISTKPGSPLKIFKNLRVCSDCHTVTKLISKITGRKIVMRDRNRFHHFTD 627

BLAST of Lsi06G001230 vs. TrEMBL
Match: A0A0A0LWF1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G569490 PE=4 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 3.4e-310
Identity = 522/577 (90.47%), Postives = 547/577 (94.80%), Query Frame = 1

Query: 1   MASLLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLI 60
           MASLL LHP  SLPNS K   SPIFH+L+SCSSM+ELKQFHSQIIR GLSTDN+AIGRLI
Sbjct: 1   MASLLPLHPIPSLPNSTKFNPSPIFHSLSSCSSMSELKQFHSQIIRLGLSTDNNAIGRLI 60

Query: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPN 120
           KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYL F SP +SLLLYLQMLHNSV PN
Sbjct: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLHFNSPKSSLLLYLQMLHNSVFPN 120

Query: 121 KFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFD 180
           KFTFPS+IRACCI N+V+EGKQIH HV+KFGF  DRF  NNLIHMYANFQSLE+A RVFD
Sbjct: 121 KFTFPSVIRACCIDNSVEEGKQIHTHVVKFGFSKDRFCQNNLIHMYANFQSLEDARRVFD 180

Query: 181 SIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240
            IELPDVVAWTTLLTGYAQ G+VDE+LRVFESMPERNSASWNAMISCFVQNNRFHEAFGL
Sbjct: 181 CIELPDVVAWTTLLTGYAQLGYVDESLRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240

Query: 241 FNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCK 300
           FNRMR+EKVVLEKYVAASMLSACTGLGALEQGKWIHRYI+RNGIE DSKLATTLIDMYCK
Sbjct: 241 FNRMRIEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIERNGIEFDSKLATTLIDMYCK 300

Query: 301 CGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNV 360
           CGCLDCA++VF HLP+KGISSWNCMIGGMAMHGKGEAAIELFK+METK+VKPDNITFLNV
Sbjct: 301 CGCLDCAYEVFVHLPEKGISSWNCMIGGMAMHGKGEAAIELFKDMETKMVKPDNITFLNV 360

Query: 361 LSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSP 420
           LSACAHSGLVEKG++YF RFTQVY IEP TEHYGCMVDLYGRAG+LEEAMKVIDEMPMSP
Sbjct: 361 LSACAHSGLVEKGQHYFYRFTQVYGIEPRTEHYGCMVDLYGRAGLLEEAMKVIDEMPMSP 420

Query: 421 DVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRK 480
           DVGVLGAFVGACKIHGNIELGEE+GKRVIELEPTNSGRYVLLGNLYAEAGRWE VAEVRK
Sbjct: 421 DVGVLGAFVGACKIHGNIELGEEVGKRVIELEPTNSGRYVLLGNLYAEAGRWEGVAEVRK 480

Query: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGI 540
           LMNDREVKKAAGVSMIELEGVVYEFIAGGR HPEAKEIY KLNEMLECIR+EGYVAEN I
Sbjct: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRNHPEAKEIYDKLNEMLECIRSEGYVAENEI 540

Query: 541 EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLR 578
           EEEKDNPVYYHSEKLAIAFGLLKTKAGE+LRITKNLR
Sbjct: 541 EEEKDNPVYYHSEKLAIAFGLLKTKAGEILRITKNLR 577

BLAST of Lsi06G001230 vs. TrEMBL
Match: F6H9I8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0069g00930 PE=4 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 1.1e-263
Identity = 444/622 (71.38%), Postives = 524/622 (84.24%), Query Frame = 1

Query: 4   LLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFC 63
           L A  P+ S   + KLP     + L+SCS+MAELKQ+HSQIIR GLS DNDA+GR+IKFC
Sbjct: 7   LQASPPSLSSAKAHKLP----LYGLDSCSTMAELKQYHSQIIRLGLSADNDAMGRVIKFC 66

Query: 64  AVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFT 123
           A+SK GDL+YAL +F+ IP+PDA+IYNT+ R YL++Q     + +Y +MLH SV PNKFT
Sbjct: 67  AISKSGDLNYALEVFDKIPHPDAYIYNTIFRGYLRWQLARNCIFMYSRMLHKSVSPNKFT 126

Query: 124 FPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIE 183
           +P LIRACCI  A++EGKQIHAHVLKFGFG D FS NNLIHMY NFQSLE+A RVFD++ 
Sbjct: 127 YPPLIRACCIDYAIEEGKQIHAHVLKFGFGADGFSLNNLIHMYVNFQSLEQARRVFDNMP 186

Query: 184 LPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNR 243
             DVV+WT+L+TGY+Q+GFVD+A  VFE MPERNS SWNAMI+ +VQ+NR HEAF LF+R
Sbjct: 187 QRDVVSWTSLITGYSQWGFVDKAREVFELMPERNSVSWNAMIAAYVQSNRLHEAFALFDR 246

Query: 244 MRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGC 303
           MRLE VVL+K+VAASMLSACTGLGALEQGKWIH YI+++GIELDSKLATT+IDMYCKCGC
Sbjct: 247 MRLENVVLDKFVAASMLSACTGLGALEQGKWIHGYIEKSGIELDSKLATTVIDMYCKCGC 306

Query: 304 LDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSA 363
           L+ A +VF  LPQKGISSWNCMIGG+AMHGKGEAAIELFKEME ++V PD ITF+NVLSA
Sbjct: 307 LEKASEVFNELPQKGISSWNCMIGGLAMHGKGEAAIELFKEMEREMVAPDGITFVNVLSA 366

Query: 364 CAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVG 423
           CAHSGLVE+G++YF   T+V  ++P  EH+GCMVDL GRAG+LEEA K+I+EMP++PD G
Sbjct: 367 CAHSGLVEEGKHYFQYMTEVLGLKPGMEHFGCMVDLLGRAGLLEEARKLINEMPVNPDAG 426

Query: 424 VLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMN 483
           VLGA VGAC+IHGN ELGE+IGK+VIELEP NSGRYVLL NLYA AGRWE VA+VRKLMN
Sbjct: 427 VLGALVGACRIHGNTELGEQIGKKVIELEPHNSGRYVLLANLYASAGRWEDVAKVRKLMN 486

Query: 484 DREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE-NGI-- 543
           DR VKKA G SMIE E  V EFIAGGR HP+AKEIYAKL+E+LE IR+ GYV + +G+  
Sbjct: 487 DRGVKKAPGFSMIESESGVDEFIAGGRAHPQAKEIYAKLDEILETIRSIGYVPDTDGVLH 546

Query: 544 ---EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKI 603
              EEEK+NP+YYHSEKLAIAFGLLKTK GE LRI+KNLR+C+DCHQA KL+SKV+ R+I
Sbjct: 547 DIDEEEKENPLYYHSEKLAIAFGLLKTKPGETLRISKNLRICRDCHQASKLISKVYDREI 606

Query: 604 IVRDRNRFHHFANGECSCNDYW 620
           I+RDRNRFHHF  G CSC DYW
Sbjct: 607 IIRDRNRFHHFRMGGCSCKDYW 624

BLAST of Lsi06G001230 vs. TrEMBL
Match: M5Y189_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa018015mg PE=4 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 6.7e-261
Identity = 439/625 (70.24%), Postives = 516/625 (82.56%), Query Frame = 1

Query: 1   MASLLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLI 60
           M SL  L  T    +SPK   SP+   + SCS+MAEL+Q HS++IR GL+ DNDA+GR+I
Sbjct: 1   MTSLQVLQATPPHLSSPKTQISPL-RGIESCSTMAELRQLHSKVIRLGLAADNDAMGRVI 60

Query: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPN 120
           KFCA+SK GDL YAL +F+++ +PDAFIYNT++R YLQ   P   ++LY QML +SV PN
Sbjct: 61  KFCALSKNGDLGYALQVFDTMLHPDAFIYNTVMRGYLQCHLPRNCIVLYSQMLQDSVTPN 120

Query: 121 KFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFD 180
           K+TFPS+IRACC  +A+ EGKQ+HAHV+K G+G D F  NNLIHMY  FQSLEEA RVFD
Sbjct: 121 KYTFPSVIRACCNDDAIGEGKQVHAHVVKLGYGADGFCQNNLIHMYVKFQSLEEARRVFD 180

Query: 181 SIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240
            +   D V+WTTL+TGY+Q GFVDEA  +FE MPE+NS SWNAMIS +VQ++RFHEAF L
Sbjct: 181 KMLRMDAVSWTTLITGYSQCGFVDEAFELFELMPEKNSVSWNAMISSYVQSDRFHEAFAL 240

Query: 241 FNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCK 300
           F +MR+EKV L+K++AASMLSACTGLGALEQGKWIH YI+++GIELDSKLATT+IDMYCK
Sbjct: 241 FQKMRVEKVELDKFMAASMLSACTGLGALEQGKWIHGYIEKSGIELDSKLATTIIDMYCK 300

Query: 301 CGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNV 360
           CGCL+ AF+VF  LP KGISSWNCMIGG+AMHGKGEAAIELF++M+  +V PDNITF+NV
Sbjct: 301 CGCLEKAFEVFNGLPHKGISSWNCMIGGLAMHGKGEAAIELFEKMQRDMVAPDNITFVNV 360

Query: 361 LSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSP 420
           LSACAHSGLVE+G+ YF    +V+ IEP  EH+GCMVDL GRAGMLEEA K+I EMPMSP
Sbjct: 361 LSACAHSGLVEEGQRYFQSMVEVHGIEPRKEHFGCMVDLLGRAGMLEEARKLISEMPMSP 420

Query: 421 DVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRK 480
           DVGVLGA +GACKIHGN+ELGE IG+ VIELEP NSGRYVLL NLYA AGRWE VA VR+
Sbjct: 421 DVGVLGALLGACKIHGNVELGEHIGRIVIELEPENSGRYVLLANLYANAGRWEDVANVRR 480

Query: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE-NG 540
           LMNDR VKK  G SMIELEGVV EFIAGG  HP+ KEIYAK++EML+CIR+ GYV +  G
Sbjct: 481 LMNDRGVKKVPGFSMIELEGVVNEFIAGGGAHPQTKEIYAKVDEMLKCIRSAGYVPDTEG 540

Query: 541 I-----EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQ 600
           +     EEEK+NP+YYHSEKLAIAFGLLKTK GE LRI+KNLRVCKDCHQA KL+SKVF 
Sbjct: 541 VLHDLDEEEKENPLYYHSEKLAIAFGLLKTKPGETLRISKNLRVCKDCHQASKLISKVFD 600

Query: 601 RKIIVRDRNRFHHFANGECSCNDYW 620
           R+IIVRDRNRFHHF  G+CSC DYW
Sbjct: 601 REIIVRDRNRFHHFKRGDCSCKDYW 624

BLAST of Lsi06G001230 vs. TrEMBL
Match: A0A061EPX5_THECC (Tetratricopeptide repeat-like superfamily protein OS=Theobroma cacao GN=TCM_021493 PE=4 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 3.5e-257
Identity = 436/624 (69.87%), Postives = 509/624 (81.57%), Query Frame = 1

Query: 4   LLALHPTASLPNSPKLPT--SPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIK 63
           LL   P +S  ++P  P   SP+  AL +CSSMA LKQ HS +I+ GLS DNDA+GR+IK
Sbjct: 6   LLQATPLSSAGHAPSKPRLHSPL-EALQTCSSMAHLKQHHSHLIKLGLSADNDAMGRIIK 65

Query: 64  FCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNK 123
           FCA+S+ G L Y L LF+++P+PDAFIYNTLIR YLQ Q PT  +L YLQML +SV PNK
Sbjct: 66  FCAISENGHLDYGLHLFDTLPHPDAFIYNTLIRGYLQRQQPTHCILFYLQMLQHSVFPNK 125

Query: 124 FTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDS 183
           FTFP LIRAC + NA+++G QIHAHV KFGF  D F  NNLIHMY NFQ+LE+A +VF+ 
Sbjct: 126 FTFPCLIRACSLANAIEQGSQIHAHVFKFGFAADTFCLNNLIHMYVNFQALEKARKVFEM 185

Query: 184 IELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLF 243
           +   DVV+WTTL++GYAQ G VDEA  +FE M ERNS SWNAMI+ +VQ+NRFHEAF LF
Sbjct: 186 MPTRDVVSWTTLISGYAQLGLVDEAFEIFELMQERNSVSWNAMIAAYVQSNRFHEAFALF 245

Query: 244 NRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKC 303
           NRMR EKVVL+K+VAASMLSACTGLGALEQGKWIH YI+ + IELD+KLATT+IDMYCKC
Sbjct: 246 NRMRAEKVVLDKFVAASMLSACTGLGALEQGKWIHGYIQDSRIELDAKLATTIIDMYCKC 305

Query: 304 GCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVL 363
           GCL+ A++ F  L  +GISSWNCMIGG AMHGK EAAI LFKEME + V PDNITF+N+L
Sbjct: 306 GCLEKAYETFKGLTCRGISSWNCMIGGFAMHGKWEAAIALFKEMEKEGVAPDNITFVNIL 365

Query: 364 SACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPD 423
           SACAHSGLVE+GRYYF   T+V++IE   EHYGCMVDL GRAG+L++A K+ID+MPMSPD
Sbjct: 366 SACAHSGLVEEGRYYFHYMTEVHAIERRMEHYGCMVDLLGRAGLLDDAKKLIDQMPMSPD 425

Query: 424 VGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKL 483
           VGVLGA  GAC+IHGNIELGE+IGKRVIELEP NSGRYVLL NLYA  GRWE VA VR++
Sbjct: 426 VGVLGALFGACRIHGNIELGEQIGKRVIELEPENSGRYVLLANLYANTGRWEDVANVRRM 485

Query: 484 MNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE-NGI 543
           MNDR VKK  G S+IELEGVV EFIAGGR H E KEIY+K++EMLECIR+ GYV +  G+
Sbjct: 486 MNDRGVKKVPGFSVIELEGVVNEFIAGGRAHSETKEIYSKVDEMLECIRSVGYVPDTEGV 545

Query: 544 -----EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQR 603
                EEE++NP+YYHSEKLAIA GLLKTK GE  RITKNLRVC+DCH A KL+SKVF R
Sbjct: 546 VHDLDEEERENPLYYHSEKLAIALGLLKTKTGETFRITKNLRVCRDCHHASKLISKVFDR 605

Query: 604 KIIVRDRNRFHHFANGECSCNDYW 620
           +IIVRDRNRFHHF +GECSC DYW
Sbjct: 606 EIIVRDRNRFHHFKDGECSCKDYW 628

BLAST of Lsi06G001230 vs. TrEMBL
Match: A0A0J8BUR1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g174460 PE=4 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 7.0e-250
Identity = 416/600 (69.33%), Postives = 499/600 (83.17%), Query Frame = 1

Query: 27  ALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGDLHYALLLFNSIPYPDA 86
           +L+ CSSMAELKQ HSQIIR GLS+DND +GR IKFCA SK  DL YA  +F+ +P+PDA
Sbjct: 25  SLDKCSSMAELKQLHSQIIRLGLSSDNDIMGRAIKFCAFSKCSDLVYAHQMFDKMPHPDA 84

Query: 87  FIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAVKEGKQIHAH 146
           FIYNTLIR YLQ Q      +LYLQML +SV+PN FTFPSLIRACCI N V++G+QIHAH
Sbjct: 85  FIYNTLIRGYLQCQLVRECFILYLQMLQDSVMPNNFTFPSLIRACCIDNWVEKGRQIHAH 144

Query: 147 VLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGYAQFGFVDEA 206
           V+K GF  D+FS NNL++MY +F  LEEA RVFD +   DVV+WTTL++GY+Q G V++A
Sbjct: 145 VVKMGFLDDKFSQNNLLYMYVSFGCLEEARRVFDKMPQRDVVSWTTLISGYSQLGLVNDA 204

Query: 207 LRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEKVVLEKYVAASMLSACTGL 266
             VF+SMP RNSA+WNAMI+ +VQN+RFHEAF LFN MR+  V L++YV ASMLSACT L
Sbjct: 205 YEVFKSMPNRNSAAWNAMIAAYVQNDRFHEAFALFNEMRMGNVELDRYVVASMLSACTKL 264

Query: 267 GALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQKGISSWNCMI 326
           GAL+QG+WIH YI++NGIE+D+KLATT+IDMYCKCGCL+ AF+VF  LP KGIS+WNCMI
Sbjct: 265 GALDQGEWIHGYIRKNGIEMDTKLATTVIDMYCKCGCLEKAFEVFNGLPSKGISTWNCMI 324

Query: 327 GGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEKGRYYFSRFTQVYSI 386
           GG+A+HG+G+AAIELFK+ME + V PDNITFLNVLSACAH+GLVE GR+YF   T+V+ +
Sbjct: 325 GGLAIHGRGKAAIELFKDMERETVAPDNITFLNVLSACAHAGLVETGRHYFKHMTEVHRL 384

Query: 387 EPVTEHYGCMVDLYGRAGMLEEAMKVI-DEMPMSPDVGVLGAFVGACKIHGNIELGEEIG 446
           EP  EHYGCMVDL GRAG+LEEA K++ +EMPM  D GVLGA VGACKIHG+I LGE  G
Sbjct: 385 EPRMEHYGCMVDLLGRAGLLEEARKLVEEEMPMEADAGVLGALVGACKIHGDINLGENFG 444

Query: 447 KRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMIELEGVVYEF 506
           KR+IEL+P NSGRYVLL NLYA AG+W  VA +RKLMNDR+VKKA G S+IE+EGVV EF
Sbjct: 445 KRLIELDPNNSGRYVLLANLYATAGKWNDVANIRKLMNDRKVKKAPGFSVIEMEGVVSEF 504

Query: 507 IAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE-NGI-----EEEKDNPVYYHSEKLAIAF 566
           IAGGR HPE+++IYAKL+EMLE I+  GYV + +G+     EEEK+NP  YHSEKLAIAF
Sbjct: 505 IAGGRAHPESRKIYAKLDEMLESIKCLGYVPDKDGLIQDIDEEEKENPSNYHSEKLAIAF 564

Query: 567 GLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFANGECSCNDYW 620
           GLLKTKAG+ +RI+KNLRVC+DCHQA KL+SK F R+IIVRDRNRFHHF NGECSCND+W
Sbjct: 565 GLLKTKAGQTIRISKNLRVCRDCHQASKLISKAFDREIIVRDRNRFHHFRNGECSCNDFW 624

BLAST of Lsi06G001230 vs. TAIR10
Match: AT5G66520.1 (AT5G66520.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 549.3 bits (1414), Expect = 3.1e-156
Identity = 273/602 (45.35%), Postives = 389/602 (64.62%), Query Frame = 1

Query: 28  LNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGD-LHYALLLFNSIPYPDA 87
           L  CS   ELKQ H+++++ GL  D+ AI + + FC  S   D L YA ++F+    PD 
Sbjct: 21  LQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQIVFDGFDRPDT 80

Query: 88  FIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAVKEGKQIHAH 147
           F++N +IR +     P  SLLLY +ML +S   N +TFPSL++AC   +A +E  QIHA 
Sbjct: 81  FLWNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQ 140

Query: 148 VLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGYAQFGFVDEA 207
           + K G+  D ++ N+LI+ YA   + + A  +FD I  PD V+W +++ GY + G +D A
Sbjct: 141 ITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPDDVSWNSVIKGYVKAGKMDIA 200

Query: 208 LRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEKVVLEKYVAASMLSACTGL 267
           L +F  M E+N+ SW  MIS +VQ +   EA  LF+ M+   V  +    A+ LSAC  L
Sbjct: 201 LTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQL 260

Query: 268 GALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQKGISSWNCMI 327
           GALEQGKWIH Y+ +  I +DS L   LIDMY KCG ++ A +VF ++ +K + +W  +I
Sbjct: 261 GALEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALI 320

Query: 328 GGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEKGRYYFSRFTQVYSI 387
            G A HG G  AI  F EM+   +KP+ ITF  VL+AC+++GLVE+G+  F    + Y++
Sbjct: 321 SGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSMERDYNL 380

Query: 388 EPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHGNIELGEEIGK 447
           +P  EHYGC+VDL GRAG+L+EA + I EMP+ P+  + GA + AC+IH NIELGEEIG+
Sbjct: 381 KPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGE 440

Query: 448 RVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMIELEGVVYEFI 507
            +I ++P + GRYV   N++A   +W++ AE R+LM ++ V K  G S I LEG  +EF+
Sbjct: 441 ILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTISLEGTTHEFL 500

Query: 508 AGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGIEE---------EKDNPVYYHSEKLAI 567
           AG R HPE ++I +K   M   +   GYV E  +EE         E++  V+ HSEKLAI
Sbjct: 501 AGDRSHPEIEKIQSKWRIMRRKLEENGYVPE--LEEMLLDLVDDDEREAIVHQHSEKLAI 560

Query: 568 AFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFANGECSCND 620
            +GL+KTK G ++RI KNLRVCKDCH+  KL+SK+++R I++RDR RFHHF +G+CSC D
Sbjct: 561 TYGLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRDIVMRDRTRFHHFRDGKCSCGD 620

BLAST of Lsi06G001230 vs. TAIR10
Match: AT5G48910.1 (AT5G48910.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 523.1 bits (1346), Expect = 2.4e-148
Identity = 256/630 (40.63%), Postives = 390/630 (61.90%), Query Frame = 1

Query: 15  NSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSK--YGDLH 74
           +SP    S +F  +N+C ++ +L Q H+  I+ G   D  A   +++FCA S   + DL 
Sbjct: 17  SSPASHPSSLFPQINNCRTIRDLSQIHAVFIKSGQMRDTLAAAEILRFCATSDLHHRDLD 76

Query: 75  YALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASL---LLYLQMLHNSVLPNKFTFPSLIR 134
           YA  +FN +P  + F +NT+IR + +     A +   L Y  M    V PN+FTFPS+++
Sbjct: 77  YAHKIFNQMPQRNCFSWNTIIRGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLK 136

Query: 135 ACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVF-------DSI 194
           AC     ++EGKQIH   LK+GFG D F  +NL+ MY     +++A  +F       D +
Sbjct: 137 ACAKTGKIQEGKQIHGLALKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMV 196

Query: 195 ELPD-------VVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFH 254
            + D       +V W  ++ GY + G    A  +F+ M +R+  SWN MIS +  N  F 
Sbjct: 197 VMTDRRKRDGEIVLWNVMIDGYMRLGDCKAARMLFDKMRQRSVVSWNTMISGYSLNGFFK 256

Query: 255 EAFGLFNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLI 314
           +A  +F  M+   +        S+L A + LG+LE G+W+H Y + +GI +D  L + LI
Sbjct: 257 DAVEVFREMKKGDIRPNYVTLVSVLPAISRLGSLELGEWLHLYAEDSGIRIDDVLGSALI 316

Query: 315 DMYCKCGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNI 374
           DMY KCG ++ A  VF  LP++ + +W+ MI G A+HG+   AI+ F +M    V+P ++
Sbjct: 317 DMYSKCGIIEKAIHVFERLPRENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDV 376

Query: 375 TFLNVLSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDE 434
            ++N+L+AC+H GLVE+GR YFS+   V  +EP  EHYGCMVDL GR+G+L+EA + I  
Sbjct: 377 AYINLLTACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILN 436

Query: 435 MPMSPDVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERV 494
           MP+ PD  +  A +GAC++ GN+E+G+ +   ++++ P +SG YV L N+YA  G W  V
Sbjct: 437 MPIKPDDVIWKALLGACRMQGNVEMGKRVANILMDMVPHDSGAYVALSNMYASQGNWSEV 496

Query: 495 AEVRKLMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGY- 554
           +E+R  M +++++K  G S+I+++GV++EF+     HP+AKEI + L E+ + +R  GY 
Sbjct: 497 SEMRLRMKEKDIRKDPGCSLIDIDGVLHEFVVEDDSHPKAKEINSMLVEISDKLRLAGYR 556

Query: 555 -----VAENGIEEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLV 614
                V  N  EE+K+N ++YHSEK+A AFGL+ T  G+ +RI KNLR+C+DCH ++KL+
Sbjct: 557 PITTQVLLNLEEEDKENVLHYHSEKIATAFGLISTSPGKPIRIVKNLRICEDCHSSIKLI 616

Query: 615 SKVFQRKIIVRDRNRFHHFANGECSCNDYW 620
           SKV++RKI VRDR RFHHF +G CSC DYW
Sbjct: 617 SKVYKRKITVRDRKRFHHFQDGSCSCMDYW 646

BLAST of Lsi06G001230 vs. TAIR10
Match: AT5G06540.1 (AT5G06540.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 504.2 bits (1297), Expect = 1.1e-142
Identity = 254/609 (41.71%), Postives = 376/609 (61.74%), Query Frame = 1

Query: 23  PIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGD-----LHYALLL 82
           P    L SCSS ++LK  H  ++R  L +D     RL+  C      +     L YA  +
Sbjct: 14  PKLALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGI 73

Query: 83  FNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAV 142
           F+ I  P+ F++N LIR +     P+ +   Y QML + + P+  TFP LI+A      V
Sbjct: 74  FSQIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECV 133

Query: 143 KEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGY 202
             G+Q H+ +++FGF  D +  N+L+HMYAN   +  A R+F  +   DVV+WT+++ GY
Sbjct: 134 LVGEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGY 193

Query: 203 AQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEKVVLEKYVAA 262
            + G V+ A  +F+ MP RN  +W+ MI+ + +NN F +A  LF  M+ E VV  + V  
Sbjct: 194 CKCGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMV 253

Query: 263 SMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQK 322
           S++S+C  LGALE G+  + Y+ ++ + ++  L T L+DM+ +CG ++ A  VF  LP+ 
Sbjct: 254 SVISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGLPET 313

Query: 323 GISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEKGRYYF 382
              SW+ +I G+A+HG    A+  F +M +    P ++TF  VLSAC+H GLVEKG   +
Sbjct: 314 DSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIY 373

Query: 383 SRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHGN 442
               + + IEP  EHYGC+VD+ GRAG L EA   I +M + P+  +LGA +GACKI+ N
Sbjct: 374 ENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKN 433

Query: 443 IELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMIE 502
            E+ E +G  +I+++P +SG YVLL N+YA AG+W+++  +R +M ++ VKK  G S+IE
Sbjct: 434 TEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIE 493

Query: 503 LEGVVYEFIAG-GRVHPEAKEIYAKLNEMLECIRTEGYVAENG------IEEEKDNPVYY 562
           ++G + +F  G  + HPE  +I  K  E+L  IR  GY    G       EEEK++ ++ 
Sbjct: 494 IDGKINKFTMGDDQKHPEMGKIRRKWEEILGKIRLIGYKGNTGDAFFDVDEEEKESSIHM 553

Query: 563 HSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFAN 620
           HSEKLAIA+G++KTK G  +RI KNLRVC+DCH   KL+S+V+ R++IVRDRNRFHHF N
Sbjct: 554 HSEKLAIAYGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFRN 613

BLAST of Lsi06G001230 vs. TAIR10
Match: AT2G29760.1 (AT2G29760.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 485.7 bits (1249), Expect = 4.2e-137
Identity = 246/614 (40.07%), Postives = 381/614 (62.05%), Query Frame = 1

Query: 14  PNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFCAVSKYGDLHY 73
           PN    P   +  A    SS++  +  H   ++  + +D      LI  C  S  GDL  
Sbjct: 129 PNKYTFPF--LIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIH-CYFS-CGDLDS 188

Query: 74  ALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCI 133
           A  +F +I   D   +N++I  ++Q  SP  +L L+ +M    V  +  T   ++ AC  
Sbjct: 189 ACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACAK 248

Query: 134 HNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTL 193
              ++ G+Q+ +++ +     +    N ++ MY    S+E+A R+FD++E  D V WTT+
Sbjct: 249 IRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTM 308

Query: 194 LTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLEK-VVLE 253
           L GYA     + A  V  SMP+++  +WNA+IS + QN + +EA  +F+ ++L+K + L 
Sbjct: 309 LDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQLQKNMKLN 368

Query: 254 KYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFT 313
           +    S LSAC  +GALE G+WIH YIK++GI ++  + + LI MY KCG L+ + +VF 
Sbjct: 369 QITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFHVTSALIHMYSKCGDLEKSREVFN 428

Query: 314 HLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSACAHSGLVEK 373
            + ++ +  W+ MIGG+AMHG G  A+++F +M+   VKP+ +TF NV  AC+H+GLV++
Sbjct: 429 SVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDE 488

Query: 374 GRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGAC 433
               F +    Y I P  +HY C+VD+ GR+G LE+A+K I+ MP+ P   V GA +GAC
Sbjct: 489 AESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGAC 548

Query: 434 KIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAG 493
           KIH N+ L E    R++ELEP N G +VLL N+YA+ G+WE V+E+RK M    +KK  G
Sbjct: 549 KIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKKEPG 608

Query: 494 VSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENG-----IEEE--KD 553
            S IE++G+++EF++G   HP ++++Y KL+E++E +++ GY  E       IEEE  K+
Sbjct: 609 CSSIEIDGMIHEFLSGDNAHPMSEKVYGKLHEVMEKLKSNGYEPEISQVLQIIEEEEMKE 668

Query: 554 NPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRF 613
             +  HSEKLAI +GL+ T+A +++R+ KNLRVC DCH   KL+S+++ R+IIVRDR RF
Sbjct: 669 QSLNLHSEKLAICYGLISTEAPKVIRVIKNLRVCGDCHSVAKLISQLYDREIIVRDRYRF 728

Query: 614 HHFANGECSCNDYW 620
           HHF NG+CSCND+W
Sbjct: 729 HHFRNGQCSCNDFW 738

BLAST of Lsi06G001230 vs. TAIR10
Match: AT4G37380.1 (AT4G37380.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 482.6 bits (1241), Expect = 3.5e-136
Identity = 256/609 (42.04%), Postives = 366/609 (60.10%), Query Frame = 1

Query: 20  PTSPIFHALNSCSSMAELKQFHSQIIRFGLSTD-NDAIGRLIKFCAVSKYGDLHYALLLF 79
           P   +   ++   S+ E+ Q H+ I+R  L       +  L    A + +G + ++L LF
Sbjct: 28  PPEKLAVLIDKSQSVDEVLQIHAAILRHNLLLHPRYPVLNLKLHRAYASHGKIRHSLALF 87

Query: 80  NSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFTFPSLIRACCIHNAVK 139
           +    PD F++   I           + LLY+Q+L + + PN+FTF SL+++C    + K
Sbjct: 88  HQTIDPDLFLFTAAINTASINGLKDQAFLLYVQLLSSEINPNEFTFSSLLKSC----STK 147

Query: 140 EGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIELPDVVAWTTLLTGYA 199
            GK IH HVLKFG G D +    L+ +YA    +  A +VFD +    +V+ T ++T YA
Sbjct: 148 SGKLIHTHVLKFGLGIDPYVATGLVDVYAKGGDVVSAQKVFDRMPERSLVSSTAMITCYA 207

Query: 200 QFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNRMRLE-KVVLEKYVAA 259
           + G V+ A  +F+SM ER+  SWN MI  + Q+   ++A  LF ++  E K   ++    
Sbjct: 208 KQGNVEAARALFDSMCERDIVSWNVMIDGYAQHGFPNDALMLFQKLLAEGKPKPDEITVV 267

Query: 260 SMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGCLDCAFQVFTHLPQK 319
           + LSAC+ +GALE G+WIH ++K + I L+ K+ T LIDMY KCG L+ A  VF   P+K
Sbjct: 268 AALSACSQIGALETGRWIHVFVKSSRIRLNVKVCTGLIDMYSKCGSLEEAVLVFNDTPRK 327

Query: 320 GISSWNCMIGGMAMHGKGEAAIELFKEMETKL-VKPDNITFLNVLSACAHSGLVEKGRYY 379
            I +WN MI G AMHG  + A+ LF EM+    ++P +ITF+  L ACAH+GLV +G   
Sbjct: 328 DIVAWNAMIAGYAMHGYSQDALRLFNEMQGITGLQPTDITFIGTLQACAHAGLVNEGIRI 387

Query: 380 FSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVGVLGAFVGACKIHG 439
           F    Q Y I+P  EHYGC+V L GRAG L+ A + I  M M  D  +  + +G+CK+HG
Sbjct: 388 FESMGQEYGIKPKIEHYGCLVSLLGRAGQLKRAYETIKNMNMDADSVLWSSVLGSCKLHG 447

Query: 440 NIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMNDREVKKAAGVSMI 499
           +  LG+EI + +I L   NSG YVLL N+YA  G +E VA+VR LM ++ + K  G+S I
Sbjct: 448 DFVLGKEIAEYLIGLNIKNSGIYVLLSNIYASVGDYEGVAKVRNLMKEKGIVKEPGISTI 507

Query: 500 ELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGI------EEEKDNPVYY 559
           E+E  V+EF AG R H ++KEIY  L ++ E I++ GYV           E EK+  +  
Sbjct: 508 EIENKVHEFRAGDREHSKSKEIYTMLRKISERIKSHGYVPNTNTVLQDLEETEKEQSLQV 567

Query: 560 HSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVRDRNRFHHFAN 619
           HSE+LAIA+GL+ TK G  L+I KNLRVC DCH   KL+SK+  RKI++RDRNRFHHF +
Sbjct: 568 HSERLAIAYGLISTKPGSPLKIFKNLRVCSDCHTVTKLISKITGRKIVMRDRNRFHHFTD 627

BLAST of Lsi06G001230 vs. NCBI nr
Match: gi|778662474|ref|XP_011659892.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Cucumis sativus])

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 563/619 (90.95%), Postives = 588/619 (94.99%), Query Frame = 1

Query: 1   MASLLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLI 60
           MASLL LHP  SLPNS K   SPIFH+L+SCSSM+ELKQFHSQIIR GLSTDN+AIGRLI
Sbjct: 1   MASLLPLHPIPSLPNSTKFNPSPIFHSLSSCSSMSELKQFHSQIIRLGLSTDNNAIGRLI 60

Query: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPN 120
           KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYL F SP +SLLLYLQMLHNSV PN
Sbjct: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLHFNSPKSSLLLYLQMLHNSVFPN 120

Query: 121 KFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFD 180
           KFTFPS+IRACCI N+V+EGKQIH HV+KFGF  DRF  NNLIHMYANFQSLE+A RVFD
Sbjct: 121 KFTFPSVIRACCIDNSVEEGKQIHTHVVKFGFSKDRFCQNNLIHMYANFQSLEDARRVFD 180

Query: 181 SIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240
            IELPDVVAWTTLLTGYAQ G+VDE+LRVFESMPERNSASWNAMISCFVQNNRFHEAFGL
Sbjct: 181 CIELPDVVAWTTLLTGYAQLGYVDESLRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240

Query: 241 FNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCK 300
           FNRMR+EKVVLEKYVAASMLSACTGLGALEQGKWIHRYI+RNGIE DSKLATTLIDMYCK
Sbjct: 241 FNRMRIEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIERNGIEFDSKLATTLIDMYCK 300

Query: 301 CGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNV 360
           CGCLDCA++VF HLP+KGISSWNCMIGGMAMHGKGEAAIELFK+METK+VKPDNITFLNV
Sbjct: 301 CGCLDCAYEVFVHLPEKGISSWNCMIGGMAMHGKGEAAIELFKDMETKMVKPDNITFLNV 360

Query: 361 LSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSP 420
           LSACAHSGLVEKG++YF RFTQVY IEP TEHYGCMVDLYGRAG+LEEAMKVIDEMPMSP
Sbjct: 361 LSACAHSGLVEKGQHYFYRFTQVYGIEPRTEHYGCMVDLYGRAGLLEEAMKVIDEMPMSP 420

Query: 421 DVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRK 480
           DVGVLGAFVGACKIHGNIELGEE+GKRVIELEPTNSGRYVLLGNLYAEAGRWE VAEVRK
Sbjct: 421 DVGVLGAFVGACKIHGNIELGEEVGKRVIELEPTNSGRYVLLGNLYAEAGRWEGVAEVRK 480

Query: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGI 540
           LMNDREVKKAAGVSMIELEGVVYEFIAGGR HPEAKEIY KLNEMLECIR+EGYVAEN I
Sbjct: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRNHPEAKEIYDKLNEMLECIRSEGYVAENEI 540

Query: 541 EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKIIVR 600
           EEEKDNPVYYHSEKLAIAFGLLKTKAGE+LRITKNLRVCKDCHQALKLVSKVFQRKIIVR
Sbjct: 541 EEEKDNPVYYHSEKLAIAFGLLKTKAGEILRITKNLRVCKDCHQALKLVSKVFQRKIIVR 600

Query: 601 DRNRFHHFANGECSCNDYW 620
           DRNRFHHF NGECSCNDYW
Sbjct: 601 DRNRFHHFGNGECSCNDYW 619

BLAST of Lsi06G001230 vs. NCBI nr
Match: gi|700210977|gb|KGN66073.1| (hypothetical protein Csa_1G569490 [Cucumis sativus])

HSP 1 Score: 1071.6 bits (2770), Expect = 4.8e-310
Identity = 522/577 (90.47%), Postives = 547/577 (94.80%), Query Frame = 1

Query: 1   MASLLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLI 60
           MASLL LHP  SLPNS K   SPIFH+L+SCSSM+ELKQFHSQIIR GLSTDN+AIGRLI
Sbjct: 1   MASLLPLHPIPSLPNSTKFNPSPIFHSLSSCSSMSELKQFHSQIIRLGLSTDNNAIGRLI 60

Query: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPN 120
           KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYL F SP +SLLLYLQMLHNSV PN
Sbjct: 61  KFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLHFNSPKSSLLLYLQMLHNSVFPN 120

Query: 121 KFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFD 180
           KFTFPS+IRACCI N+V+EGKQIH HV+KFGF  DRF  NNLIHMYANFQSLE+A RVFD
Sbjct: 121 KFTFPSVIRACCIDNSVEEGKQIHTHVVKFGFSKDRFCQNNLIHMYANFQSLEDARRVFD 180

Query: 181 SIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240
            IELPDVVAWTTLLTGYAQ G+VDE+LRVFESMPERNSASWNAMISCFVQNNRFHEAFGL
Sbjct: 181 CIELPDVVAWTTLLTGYAQLGYVDESLRVFESMPERNSASWNAMISCFVQNNRFHEAFGL 240

Query: 241 FNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCK 300
           FNRMR+EKVVLEKYVAASMLSACTGLGALEQGKWIHRYI+RNGIE DSKLATTLIDMYCK
Sbjct: 241 FNRMRIEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIERNGIEFDSKLATTLIDMYCK 300

Query: 301 CGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNV 360
           CGCLDCA++VF HLP+KGISSWNCMIGGMAMHGKGEAAIELFK+METK+VKPDNITFLNV
Sbjct: 301 CGCLDCAYEVFVHLPEKGISSWNCMIGGMAMHGKGEAAIELFKDMETKMVKPDNITFLNV 360

Query: 361 LSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSP 420
           LSACAHSGLVEKG++YF RFTQVY IEP TEHYGCMVDLYGRAG+LEEAMKVIDEMPMSP
Sbjct: 361 LSACAHSGLVEKGQHYFYRFTQVYGIEPRTEHYGCMVDLYGRAGLLEEAMKVIDEMPMSP 420

Query: 421 DVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRK 480
           DVGVLGAFVGACKIHGNIELGEE+GKRVIELEPTNSGRYVLLGNLYAEAGRWE VAEVRK
Sbjct: 421 DVGVLGAFVGACKIHGNIELGEEVGKRVIELEPTNSGRYVLLGNLYAEAGRWEGVAEVRK 480

Query: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAENGI 540
           LMNDREVKKAAGVSMIELEGVVYEFIAGGR HPEAKEIY KLNEMLECIR+EGYVAEN I
Sbjct: 481 LMNDREVKKAAGVSMIELEGVVYEFIAGGRNHPEAKEIYDKLNEMLECIRSEGYVAENEI 540

Query: 541 EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLR 578
           EEEKDNPVYYHSEKLAIAFGLLKTKAGE+LRITKNLR
Sbjct: 541 EEEKDNPVYYHSEKLAIAFGLLKTKAGEILRITKNLR 577

BLAST of Lsi06G001230 vs. NCBI nr
Match: gi|657996239|ref|XP_008390483.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Malus domestica])

HSP 1 Score: 917.5 bits (2370), Expect = 1.2e-263
Identity = 442/628 (70.38%), Postives = 521/628 (82.96%), Query Frame = 1

Query: 1   MASLLALHPTASLP---NSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIG 60
           M SLL L PT   P   +SP+   SP+   L SCS+MAELKQ HSQ+IR GL+ DNDA+G
Sbjct: 1   MTSLLVLQPTGPTPASLSSPRTHISPL-RGLESCSTMAELKQLHSQVIRLGLAADNDAMG 60

Query: 61  RLIKFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSV 120
           R+IKFCA+SK GDL YAL +F+++P PDAFIYNT++R YLQ+Q P   ++LY QML + V
Sbjct: 61  RVIKFCALSKNGDLGYALQVFDAMPQPDAFIYNTVMRGYLQYQLPRDCIVLYSQMLQDFV 120

Query: 121 LPNKFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATR 180
            PN+FTFPS++RACC   AV EGKQ+HAHV+K GFG D F  NNLIHMY  FQSL+EA R
Sbjct: 121 TPNRFTFPSVVRACCADGAVGEGKQVHAHVVKLGFGDDGFCQNNLIHMYVKFQSLDEARR 180

Query: 181 VFDSIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEA 240
           VFD +   DVV+WTTL+ GY+Q+GFVDEA  +FE MPE+NS SWNAMIS +VQ++RFHEA
Sbjct: 181 VFDKMPRVDVVSWTTLIAGYSQWGFVDEAFEMFELMPEKNSVSWNAMISSYVQSDRFHEA 240

Query: 241 FGLFNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDM 300
           F LF RMR+E V L+K++AASMLSACTGLGALEQGKWIH YI+++GIELDSKLATT+IDM
Sbjct: 241 FALFQRMRVENVELDKFMAASMLSACTGLGALEQGKWIHGYIEKSGIELDSKLATTIIDM 300

Query: 301 YCKCGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITF 360
           YCKCGCL+ AF+VF  LP+KGISSWNCMIGG+AMHGKGEAA+ELF++M+  +V PDNITF
Sbjct: 301 YCKCGCLEKAFEVFNGLPRKGISSWNCMIGGLAMHGKGEAAVELFEQMQRDMVAPDNITF 360

Query: 361 LNVLSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMP 420
           +NVLSACAHSGLVEKG+ YF    +V+ IEP TEH+GCMVDL GRAG LEEA K+I+EMP
Sbjct: 361 VNVLSACAHSGLVEKGQQYFRCMVEVHGIEPRTEHFGCMVDLLGRAGRLEEARKLINEMP 420

Query: 421 MSPDVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAE 480
           MSPDVGVLGA +GACKIHGN+ELG+EIG+RVIELEP NSGRYVLL NLYA AGRW+ VA 
Sbjct: 421 MSPDVGVLGALLGACKIHGNVELGDEIGRRVIELEPENSGRYVLLANLYANAGRWDDVAN 480

Query: 481 VRKLMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE 540
           VR+LMNDR VKK  G SMIELEG V EFIAGG  HP+ KEIY+K++EML+CIR+ GYV +
Sbjct: 481 VRRLMNDRGVKKVPGFSMIELEGAVSEFIAGGGSHPQTKEIYSKVDEMLKCIRSAGYVPD 540

Query: 541 -NGI-----EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSK 600
             G+     EEEK+NP+YYHSEKLAIA+GLLKTK  E LRI+KNLRVCKDCHQA KL+SK
Sbjct: 541 TEGVLHDLDEEEKENPLYYHSEKLAIAYGLLKTKPRETLRISKNLRVCKDCHQASKLISK 600

Query: 601 VFQRKIIVRDRNRFHHFANGECSCNDYW 620
           VF R+IIVRDRNRFHHF  GECSC DYW
Sbjct: 601 VFDREIIVRDRNRFHHFKGGECSCKDYW 627

BLAST of Lsi06G001230 vs. NCBI nr
Match: gi|225466163|ref|XP_002263755.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Vitis vinifera])

HSP 1 Score: 917.1 bits (2369), Expect = 1.6e-263
Identity = 444/622 (71.38%), Postives = 524/622 (84.24%), Query Frame = 1

Query: 4   LLALHPTASLPNSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIGRLIKFC 63
           L A  P+ S   + KLP     + L+SCS+MAELKQ+HSQIIR GLS DNDA+GR+IKFC
Sbjct: 7   LQASPPSLSSAKAHKLP----LYGLDSCSTMAELKQYHSQIIRLGLSADNDAMGRVIKFC 66

Query: 64  AVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSVLPNKFT 123
           A+SK GDL+YAL +F+ IP+PDA+IYNT+ R YL++Q     + +Y +MLH SV PNKFT
Sbjct: 67  AISKSGDLNYALEVFDKIPHPDAYIYNTIFRGYLRWQLARNCIFMYSRMLHKSVSPNKFT 126

Query: 124 FPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATRVFDSIE 183
           +P LIRACCI  A++EGKQIHAHVLKFGFG D FS NNLIHMY NFQSLE+A RVFD++ 
Sbjct: 127 YPPLIRACCIDYAIEEGKQIHAHVLKFGFGADGFSLNNLIHMYVNFQSLEQARRVFDNMP 186

Query: 184 LPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEAFGLFNR 243
             DVV+WT+L+TGY+Q+GFVD+A  VFE MPERNS SWNAMI+ +VQ+NR HEAF LF+R
Sbjct: 187 QRDVVSWTSLITGYSQWGFVDKAREVFELMPERNSVSWNAMIAAYVQSNRLHEAFALFDR 246

Query: 244 MRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDMYCKCGC 303
           MRLE VVL+K+VAASMLSACTGLGALEQGKWIH YI+++GIELDSKLATT+IDMYCKCGC
Sbjct: 247 MRLENVVLDKFVAASMLSACTGLGALEQGKWIHGYIEKSGIELDSKLATTVIDMYCKCGC 306

Query: 304 LDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITFLNVLSA 363
           L+ A +VF  LPQKGISSWNCMIGG+AMHGKGEAAIELFKEME ++V PD ITF+NVLSA
Sbjct: 307 LEKASEVFNELPQKGISSWNCMIGGLAMHGKGEAAIELFKEMEREMVAPDGITFVNVLSA 366

Query: 364 CAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMPMSPDVG 423
           CAHSGLVE+G++YF   T+V  ++P  EH+GCMVDL GRAG+LEEA K+I+EMP++PD G
Sbjct: 367 CAHSGLVEEGKHYFQYMTEVLGLKPGMEHFGCMVDLLGRAGLLEEARKLINEMPVNPDAG 426

Query: 424 VLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAEVRKLMN 483
           VLGA VGAC+IHGN ELGE+IGK+VIELEP NSGRYVLL NLYA AGRWE VA+VRKLMN
Sbjct: 427 VLGALVGACRIHGNTELGEQIGKKVIELEPHNSGRYVLLANLYASAGRWEDVAKVRKLMN 486

Query: 484 DREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE-NGI-- 543
           DR VKKA G SMIE E  V EFIAGGR HP+AKEIYAKL+E+LE IR+ GYV + +G+  
Sbjct: 487 DRGVKKAPGFSMIESESGVDEFIAGGRAHPQAKEIYAKLDEILETIRSIGYVPDTDGVLH 546

Query: 544 ---EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSKVFQRKI 603
              EEEK+NP+YYHSEKLAIAFGLLKTK GE LRI+KNLR+C+DCHQA KL+SKV+ R+I
Sbjct: 547 DIDEEEKENPLYYHSEKLAIAFGLLKTKPGETLRISKNLRICRDCHQASKLISKVYDREI 606

Query: 604 IVRDRNRFHHFANGECSCNDYW 620
           I+RDRNRFHHF  G CSC DYW
Sbjct: 607 IIRDRNRFHHFRMGGCSCKDYW 624

BLAST of Lsi06G001230 vs. NCBI nr
Match: gi|694387951|ref|XP_009369706.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Pyrus x bretschneideri])

HSP 1 Score: 916.4 bits (2367), Expect = 2.7e-263
Identity = 444/628 (70.70%), Postives = 519/628 (82.64%), Query Frame = 1

Query: 1   MASLLALHPTASLP---NSPKLPTSPIFHALNSCSSMAELKQFHSQIIRFGLSTDNDAIG 60
           M SL  L PT   P   +SP+   SPI   L SCS+MAELKQ HSQ+IR GL+TDNDA+G
Sbjct: 1   MNSLQVLQPTGPTPPSLSSPRTHISPI-RGLESCSTMAELKQLHSQVIRLGLATDNDAMG 60

Query: 61  RLIKFCAVSKYGDLHYALLLFNSIPYPDAFIYNTLIRAYLQFQSPTASLLLYLQMLHNSV 120
           R+IKFCA+SK GDL YAL +F+++P PDAFIYNT++R YLQ Q P   ++LY QML + V
Sbjct: 61  RVIKFCALSKNGDLGYALQVFDAMPQPDAFIYNTVMRGYLQCQLPRDCIVLYSQMLQDFV 120

Query: 121 LPNKFTFPSLIRACCIHNAVKEGKQIHAHVLKFGFGTDRFSNNNLIHMYANFQSLEEATR 180
            PN+FTFPS++RACC   AV EGKQ+HAHV+K GFG D F  NNLIHMY  FQSLEEA R
Sbjct: 121 TPNRFTFPSVVRACCADGAVVEGKQVHAHVIKLGFGDDGFCQNNLIHMYVKFQSLEEARR 180

Query: 181 VFDSIELPDVVAWTTLLTGYAQFGFVDEALRVFESMPERNSASWNAMISCFVQNNRFHEA 240
           VFD +   DVV+WTTL+TGY+Q GFVDEA  +FE MPE+NS SWNAMIS +VQ+ RFHEA
Sbjct: 181 VFDKMPRVDVVSWTTLITGYSQRGFVDEAFEMFELMPEKNSVSWNAMISSYVQSGRFHEA 240

Query: 241 FGLFNRMRLEKVVLEKYVAASMLSACTGLGALEQGKWIHRYIKRNGIELDSKLATTLIDM 300
           F LF RMR+E V L+K++AASMLSACTGLGALEQGKWIH YI+++GIELDSKLATT+IDM
Sbjct: 241 FALFQRMRVENVELDKFMAASMLSACTGLGALEQGKWIHGYIEKSGIELDSKLATTIIDM 300

Query: 301 YCKCGCLDCAFQVFTHLPQKGISSWNCMIGGMAMHGKGEAAIELFKEMETKLVKPDNITF 360
           YCKCGCL+ AF+VF  LP+KGISSWNCMIGG+AMHGKGEAA+ELF++M+  +V PDNITF
Sbjct: 301 YCKCGCLEKAFEVFNGLPRKGISSWNCMIGGLAMHGKGEAAVELFEQMQRDMVAPDNITF 360

Query: 361 LNVLSACAHSGLVEKGRYYFSRFTQVYSIEPVTEHYGCMVDLYGRAGMLEEAMKVIDEMP 420
           +NVLSACAHSGLVEKG+ YF    +V+ IEP TEH+GCMVDL GRAG LEEA K+I+EMP
Sbjct: 361 VNVLSACAHSGLVEKGQQYFRSMVEVHGIEPRTEHFGCMVDLLGRAGRLEEARKLINEMP 420

Query: 421 MSPDVGVLGAFVGACKIHGNIELGEEIGKRVIELEPTNSGRYVLLGNLYAEAGRWERVAE 480
           MSP+VGVLGA +GACKIHGN+ELG+EIG+RVIELEP NSGRYVLL NLYA AGRW+ VA 
Sbjct: 421 MSPNVGVLGALLGACKIHGNVELGDEIGRRVIELEPENSGRYVLLANLYANAGRWDNVAN 480

Query: 481 VRKLMNDREVKKAAGVSMIELEGVVYEFIAGGRVHPEAKEIYAKLNEMLECIRTEGYVAE 540
           VR+LMNDR VKK  G SMIELEG V EFIAGG  HP+ KEIY+K++EML+CIR+ GYV +
Sbjct: 481 VRRLMNDRGVKKVPGFSMIELEGTVSEFIAGGGSHPQTKEIYSKVDEMLKCIRSAGYVPD 540

Query: 541 -NGI-----EEEKDNPVYYHSEKLAIAFGLLKTKAGEMLRITKNLRVCKDCHQALKLVSK 600
             G+     EEE++NP+YYHSEKLAIAFGLLKTK  E LRI+KNLRVCKDCHQA KL+SK
Sbjct: 541 TEGVLHDLDEEERENPLYYHSEKLAIAFGLLKTKPRETLRISKNLRVCKDCHQASKLISK 600

Query: 601 VFQRKIIVRDRNRFHHFANGECSCNDYW 620
           VF R+IIVRDRNRFHHF  GECSC DYW
Sbjct: 601 VFDREIIVRDRNRFHHFKGGECSCKDYW 627

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP449_ARATH5.5e-15545.35Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana GN... [more]
PP425_ARATH4.2e-14740.63Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana GN... [more]
PP367_ARATH2.0e-14141.71Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana GN... [more]
PP175_ARATH7.4e-13640.07Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
PP354_ARATH6.3e-13542.04Pentatricopeptide repeat-containing protein ELI1, chloroplastic OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A0A0LWF1_CUCSA3.4e-31090.47Uncharacterized protein OS=Cucumis sativus GN=Csa_1G569490 PE=4 SV=1[more]
F6H9I8_VITVI1.1e-26371.38Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0069g00930 PE=4 SV=... [more]
M5Y189_PRUPE6.7e-26170.24Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa018015mg PE=4 SV=1[more]
A0A061EPX5_THECC3.5e-25769.87Tetratricopeptide repeat-like superfamily protein OS=Theobroma cacao GN=TCM_0214... [more]
A0A0J8BUR1_BETVU7.0e-25069.33Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g174460 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT5G66520.13.1e-15645.35 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT5G48910.12.4e-14840.63 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT5G06540.11.1e-14241.71 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT2G29760.14.2e-13740.07 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT4G37380.13.5e-13642.04 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778662474|ref|XP_011659892.1|0.0e+0090.95PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Cucumis s... [more]
gi|700210977|gb|KGN66073.1|4.8e-31090.47hypothetical protein Csa_1G569490 [Cucumis sativus][more]
gi|657996239|ref|XP_008390483.1|1.2e-26370.38PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Malus dom... [more]
gi|225466163|ref|XP_002263755.1|1.6e-26371.38PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Vitis vin... [more]
gi|694387951|ref|XP_009369706.1|2.7e-26370.70PREDICTED: pentatricopeptide repeat-containing protein At5g66520-like [Pyrus x b... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011990TPR-like_helical_dom_sf
IPR002885Pentatricopeptide_repeat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G001230.1Lsi06G001230.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 392..417
score: 1.6E-5coord: 188..217
score: 5.4E-7coord: 220..247
score: 1.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 84..132
score: 7.6E-8coord: 319..365
score: 2.5
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 292..319
score: 0.0027coord: 393..416
score: 5.3E-5coord: 220..250
score: 6.4E-8coord: 188..217
score: 4.3E-5coord: 321..353
score: 7.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 186..220
score: 11.224coord: 221..251
score: 7.618coord: 120..154
score: 7.761coord: 318..352
score: 10.369coord: 353..388
score: 6.566coord: 287..317
score: 7.859coord: 85..119
score: 8.977coord: 155..185
score: 6.193coord: 389..419
score: 8.364coord: 252..286
score: 6.906coord: 455..489
score:
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 392..474
score: 7.3E-8coord: 186..274
score: 7.
IPR011990Tetratricopeptide-like helical domainunknownSSF48452TPR-likecoord: 197..241
score: 2.28E-5coord: 436..474
score: 2.2
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 1..496
score: 3.6E
NoneNo IPR availablePANTHERPTHR24015:SF615SUBFAMILY NOT NAMEDcoord: 1..496
score: 3.6E