Lsi06G001160 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G001160
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Heavy metal transport/detoxification superfamily protein, putative
Location	chr06 : 1128411 .. 1130002 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATTGCTTACTCTCTATAACATGACTTCTTTCATCAAATTTTGACCTTTGATTGTGTTCAAATCCAAAATTTCACTCAATTTCTCATCTGTGAATTATTTGTTTTTTTCCCTTAAAAAAATTGTCTTTTCATTTCTTTACAATTTGATCACTGTTTTATTTCAAGAATCCAAACTTTCCCTCATTTTCTCATCTGGGTATCTTTTATCTTCTCAATAAGATAATTTTCTCAAAATAATTCCCTTTATTTCTCCAAATTTTAATCTTTAGTTCCTTCTAATCCAAAATTTCTCCCATTTTCTCATCTGGGTGTTCTTCAGTTACTTTGTAATCATCATTTTTCTTCTTCAAATCCTTCAAATTTGTTAAAAATTTCTTTGAAATCATCAGGAGTTCACGCAATAGAAATCGATCGAAAGCAACACAAAGTCACAGTCACAACAAACACTGACGAACAAACTCTGATCAAAAGATTGATCAAGGCAGGAAAGCACGCAGAACCATGGCCAGAAATAAAACCCATCTCAAAAAACATCAAAGAAAAGCAAATCGCCGTAGAAATCCCCGCCGGGGAAACTAGCGCCGGAAAGCAGAAACAGAGCACTGAAACAGAGCCACCGGCGGAAGAATTGCAAGTCCCATCGAAAAATGAAGAGAAATGTGGAATTAATGAGAATGTCCAAGTAATTGAAATTAGCAAGGGCGGCGATGGCCACGGAGATGCGACGGTGACCGGCGGAGGCGGCGGCCCTGCAGAACGAGCTGTGGAGTCTTCACCGAGCATCGCCGGTGAGACTGAGCCCGGTGTGCCGAGCGTTGCGGTGGCTGTTGAGGCAAGCTGCAGTGGCGGTGGAGAAGGGAGGAAGAAGAAGAAGAAGAAACCGCAGAGGAAAGAGAAGAGCGGCGGCGCATCCGCCGGAGAGGTGGTGAGTCCACAGACAGTTCCTACACCTGCAAGCATAGGATTAGCCACTCCACTTGATCATCACCATGATCAAATGTCATCGTCCAATCACAGCCCTCCATTTAATCAGCCATACAATTATCCAGTACACACGGCCGTATCACAACCAGCTTACGTGGCAAGTTACAATACCGTATATCCAAGTAGCACCTACGGTGCATACTACGCTTCGCCGCCACCATATTCGTATGCTTATGTACATTCAGCTGCACCAAGAAATATCTCGTCGTTGTCGTCATCATCGCCAGTCATGGAACAATCGTATGCTCACGTACATTCTGTTGCATCGAGAAATATCTCATCACTATCGTCGCCAGAAGTAGAACAATCAAACTCTCCACCATCAAGTCCGTTCGACTTCTTTAGCGATGAGAATCCGAGTGGTTGTTCTATCATGTGACACGAAAAAAATTATACTTAGTAGAACGATAATAAGCTTATACGATTTGTATGAGTATATGGGAGTAGAATTGTAATATATACATGTATAGGGATAATGTATGAGGCCAAAAAAAGGGGAAAAGAGAAAGAAAAACAAAGAGGAAGGAAAAAAGAGGAAAAAAAGAAAACTTTGTGGGTATTTGTGCTTAGCTTTATTTAGTGTACTTCCTTTACTATTTCAAA

mRNA sequence

ATGGGAGTTCACGCAATAGAAATCGATCGAAAGCAACACAAAGTCACAGTCACAACAAACACTGACGAACAAACTCTGATCAAAAGATTGATCAAGGCAGGAAAGCACGCAGAACCATGGCCAGAAATAAAACCCATCTCAAAAAACATCAAAGAAAAGCAAATCGCCGTAGAAATCCCCGCCGGGGAAACTAGCGCCGGAAAGCAGAAACAGAGCACTGAAACAGAGCCACCGGCGGAAGAATTGCAAGTCCCATCGAAAAATGAAGAGAAATGTGGAATTAATGAGAATGTCCAAGTAATTGAAATTAGCAAGGGCGGCGATGGCCACGGAGATGCGACGGTGACCGGCGGAGGCGGCGGCCCTGCAGAACGAGCTGTGGAGTCTTCACCGAGCATCGCCGGTGAGACTGAGCCCGGTGTGCCGAGCGTTGCGGTGGCTGTTGAGGCAAGCTGCAGTGGCGGTGGAGAAGGGAGGAAGAAGAAGAAGAAGAAACCGCAGAGGAAAGAGAAGAGCGGCGGCGCATCCGCCGGAGAGGTGGTGAGTCCACAGACAGTTCCTACACCTGCAAGCATAGGATTAGCCACTCCACTTGATCATCACCATGATCAAATGTCATCGTCCAATCACAGCCCTCCATTTAATCAGCCATACAATTATCCAGTACACACGGCCGTATCACAACCAGCTTACGTGGCAAGTTACAATACCGTATATCCAAGTAGCACCTACGGTGCATACTACGCTTCGCCGCCACCATATTCGTATGCTTATGTACATTCAGCTGCACCAAGAAATATCTCGTCGTTGTCGTCATCATCGCCAGTCATGGAACAATCGTATGCTCACGTACATTCTGTTGCATCGAGAAATATCTCATCACTATCGTCGCCAGAAGTAGAACAATCAAACTCTCCACCATCAAGTCCGTTCGACTTCTTTAGCGATGAGAATCCGAGTGGTTGTTCTATCATGTGACACGAAAAAAATTATACTTAGTAGAACGATAATAAGCTTATACGATTTGTATGAGTATATGGGAGTAGAATTGTAATATATACATGTATAGGGATAATGTATGAGGCCAAAAAAAGGGGAAAAGAGAAAGAAAAACAAAGAGGAAGGAAAAAAGAGGAAAAAAAGAAAACTTTGTGGGTATTTGTGCTTAGCTTTATTTAGTGTACTTCCTTTACTATTTCAAA

Coding sequence (CDS)

ATGGGAGTTCACGCAATAGAAATCGATCGAAAGCAACACAAAGTCACAGTCACAACAAACACTGACGAACAAACTCTGATCAAAAGATTGATCAAGGCAGGAAAGCACGCAGAACCATGGCCAGAAATAAAACCCATCTCAAAAAACATCAAAGAAAAGCAAATCGCCGTAGAAATCCCCGCCGGGGAAACTAGCGCCGGAAAGCAGAAACAGAGCACTGAAACAGAGCCACCGGCGGAAGAATTGCAAGTCCCATCGAAAAATGAAGAGAAATGTGGAATTAATGAGAATGTCCAAGTAATTGAAATTAGCAAGGGCGGCGATGGCCACGGAGATGCGACGGTGACCGGCGGAGGCGGCGGCCCTGCAGAACGAGCTGTGGAGTCTTCACCGAGCATCGCCGGTGAGACTGAGCCCGGTGTGCCGAGCGTTGCGGTGGCTGTTGAGGCAAGCTGCAGTGGCGGTGGAGAAGGGAGGAAGAAGAAGAAGAAGAAACCGCAGAGGAAAGAGAAGAGCGGCGGCGCATCCGCCGGAGAGGTGGTGAGTCCACAGACAGTTCCTACACCTGCAAGCATAGGATTAGCCACTCCACTTGATCATCACCATGATCAAATGTCATCGTCCAATCACAGCCCTCCATTTAATCAGCCATACAATTATCCAGTACACACGGCCGTATCACAACCAGCTTACGTGGCAAGTTACAATACCGTATATCCAAGTAGCACCTACGGTGCATACTACGCTTCGCCGCCACCATATTCGTATGCTTATGTACATTCAGCTGCACCAAGAAATATCTCGTCGTTGTCGTCATCATCGCCAGTCATGGAACAATCGTATGCTCACGTACATTCTGTTGCATCGAGAAATATCTCATCACTATCGTCGCCAGAAGTAGAACAATCAAACTCTCCACCATCAAGTCCGTTCGACTTCTTTAGCGATGAGAATCCGAGTGGTTGTTCTATCATGTGA

Protein sequence

MGVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKNIKEKQIAVEIPAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVTGGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGGGEGRKKKKKKPQRKEKSGGASAGEVVSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHTAVSQPAYVASYNTVYPSSTYGAYYASPPPYSYAYVHSAAPRNISSLSSSSPVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM

Homology

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BLAST of Lsi06G001160 vs. TrEMBL
Match: A0A0A0LZ09_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G569440 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.5e-107
Identity = 231/335 (68.96%), Postives = 262/335 (78.21%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKNIKEKQIAVEIPA 61
           GVH++EIDRKQ KVT+TTN DEQ+LIKRLIKAG HAEPWPE KPISK IKEKQI VEIP 
Sbjct: 45  GVHSVEIDRKQQKVTITTNIDEQSLIKRLIKAGMHAEPWPETKPISKIIKEKQIPVEIPP 104

Query: 62  GETSA-----GKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVT 121
           GETSA     GK+KQ+TETEPPAEELQVP +NEEK G NENV+  +     DGHGDAT T
Sbjct: 105 GETSASVRDGGKKKQTTETEPPAEELQVPPRNEEKSGTNENVRRCD-----DGHGDATET 164

Query: 122 GGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGGGEGRKKKKKKP----QRKEKS 181
           GG   P ER VES P+I+ ET+PG+PS AV +EASCSG GE  +KKKKK     QRKEK+
Sbjct: 165 GG---PVERVVESPPNISSETQPGMPSGAVDIEASCSGDGEVMRKKKKKKKAQAQRKEKN 224

Query: 182 GGASAGEVVSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHTAVSQPAYV 241
            GA AGE+VSPQTVPTP +IG  TP     +Q+ SSNHSPPFN    +P+HT +SQPAY+
Sbjct: 225 SGAVAGEMVSPQTVPTPTNIGSPTP----PNQIPSSNHSPPFN----HPLHTTLSQPAYI 284

Query: 242 ASYNTVYPSSTYGAYYASPPPYSYAYVHSAAPRNISSLSSSSPVMEQSYAHVHSVASRNI 301
           ASYNT YP++T+ AYYASPP YSYAYVHS AP    +LSSS P+MEQ YA+ HS+  RNI
Sbjct: 285 ASYNTAYPTNTHDAYYASPPSYSYAYVHSMAP----TLSSSLPIMEQPYAYAHSIEPRNI 344

Query: 302 SSLSS--PEVEQSNSPPSSPFDFFSDENPSGCSIM 326
           S LSS  P VEQSNSPPSSPFDFFSDENPSGCSIM
Sbjct: 345 SPLSSLPPVVEQSNSPPSSPFDFFSDENPSGCSIM 359

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BLAST of Lsi06G001160 vs. TrEMBL
Match: A5AP55_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_004114 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.9e-23
Identity = 124/351 (35.33%), Postives = 159/351 (45.30%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEI-----KPISKNIKEKQIA 61
           GV+  EID +Q KV VT N D +TL+K+L+K GKHAE WPE      +  SKN KEKQ  
Sbjct: 47  GVYTTEIDTRQQKVCVTGNVDVETLLKKLVKNGKHAELWPEKADHQKEKKSKN-KEKQKD 106

Query: 62  VEIPAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVT 121
            E    E   G  K+ +  +   E +Q P+KN E    +        S GG G G  T  
Sbjct: 107 QESNEQEGGHGGDKEKSTVK--FEAVQEPTKNPEPK--HSEAAAASGSGGGGGGGGGTKV 166

Query: 122 GGGGGPAERAV----ESSPSI-------AGETEPGVPSVAVAVEASCS--GGGEGRKKKK 181
           G  G   +       E+ P +       AGE  P         +A     G G G KKKK
Sbjct: 167 GENGAAVKNGAAAQKEAKPEVKKPEASPAGEAPPAAEKKVGESDACADKGGSGNGSKKKK 226

Query: 182 KKPQRKEKSGGASAGEV-VSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPV 241
           KK Q+     GA + +   +P   P P+      P   H    +S+         Y+YP 
Sbjct: 227 KKGQKPNSDVGAPSSDTPAAPPGSPNPS------PAPSHGPAPASTQ------TVYHYPP 286

Query: 242 HT-AVSQPAYVASYNTVYPSSTYGA-YYASPPP-YSYAYVHSAA-----PRNISSLSSSS 301
           H  A  QPAY  SYNT++PS+++GA YYA+PPP YSYAY+H        P +  S  SS 
Sbjct: 287 HYYAPPQPAYTVSYNTMHPSTSHGASYYATPPPQYSYAYMHPGPWTEPPPSDFDSNPSS- 346

Query: 302 PVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
                    VH                    PPS  F FFSDENP+ CS+M
Sbjct: 347 ---------VH--------------------PPSDSFQFFSDENPNACSLM 350

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BLAST of Lsi06G001160 vs. TrEMBL
Match: A0A067JKX9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23316 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.5e-22
Identity = 122/352 (34.66%), Postives = 155/352 (44.03%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEI-----KPISKNI-----K 61
           GV+  +ID KQ KVTV  N D  TLIK+L+KAGKHAE WPE      K  SKN      K
Sbjct: 62  GVYKTDIDLKQQKVTVLGNVDADTLIKKLVKAGKHAELWPEKADHKKKGKSKNKDKEKEK 121

Query: 62  EKQIAVEIPAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVI--------EI 121
           EKQ + +  +GE    K K++ +TE     +Q PSK  E    N+N             +
Sbjct: 122 EKQQSDQ-ESGEEGGEKNKETVKTEVLI--VQDPSKPNENGTANKNGGTTTKNGAVESNV 181

Query: 122 SKGGDGHGDATVTGGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGG----GEGR 181
             G      AT   GGG   E  +E    +        P     + +   GG    G G 
Sbjct: 182 DVGKSKEAGATSKTGGGQVKEVKIEMKQPVTCPAGTQSPVTEKKIGSENEGGAEKSGNGG 241

Query: 182 KKKKKKPQRKEKSGGASAGEVVSPQTVPTPASIGLATPLDHHHDQMS------SSNHSPP 241
           KKKKKK     K    +AGE      VP     G  +P+ H H Q        S+NHS P
Sbjct: 242 KKKKKKGH---KGNNNNAGEGEHSSDVPA----GTGSPI-HGHGQGPIGPVPYSTNHSLP 301

Query: 242 FNQPYNYPVHTAVSQPAYVASYNTVYPSSTYGAYYASPPPYSYAYVHSAAPRNISSLSSS 301
            +  Y YP H     PAY  +YNT YPS++YGA   +PP YSYAY+           +S 
Sbjct: 302 HHHVYQYPSHYYTPPPAYAVNYNTAYPSTSYGA-SINPPSYSYAYMQ-------PGFASE 361

Query: 302 SPVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
            P +                     + E + S P   F+ FSDENP+ CSIM
Sbjct: 362 PPAL---------------------DFESNPSQPLDSFEIFSDENPNACSIM 373

[Close]

BLAST of Lsi06G001160 vs. TrEMBL
Match: F6I4N0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0060g02090 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.8e-21
Identity = 116/348 (33.33%), Postives = 152/348 (43.68%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKN--IKEKQIAVEI 61
           GV+  EID +Q KV VT N D +TL+K+L+K GKHAE WPE     K    K K+   + 
Sbjct: 47  GVYTTEIDTRQQKVCVTGNVDVETLLKKLVKNGKHAELWPEKADHQKEKKSKNKEKQKDQ 106

Query: 62  PAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVTGGG 121
            + E   G      ++    E +Q P+KN E    +        S GG G G     G  
Sbjct: 107 ESNEQEGGHDGDKEKSTVKFEAVQEPTKNPEPK--HSEAAAASGSGGGGGGGGGAKVGEN 166

Query: 122 GGPAERAV----ESSPSI-------AGETEPGVPSVAVAVEASCS--GGGEGRKKKKKKP 181
           G   +       E+ P +       AGE  P         +A     G G G KKKKKK 
Sbjct: 167 GAAVKNGAAAQKEAKPEVKKPEASPAGEAPPAAEKKVGESDACADKGGSGNGSKKKKKKG 226

Query: 182 QRKEKSGGASAGEV-VSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHT- 241
           Q+     GA + +   +P   P P+      P   H    +S+         Y+YP H  
Sbjct: 227 QKPNSDVGAPSSDTPAAPPGSPNPS------PAPSHGPAPASTQ------TVYHYPPHYY 286

Query: 242 AVSQPAYVASYNTVYPSSTYGA-YYASPPP-YSYAYVHSAA-----PRNISSLSSSSPVM 301
           A  QPAY  SYNT++PS+++ A YYA+PPP YSYAY+H        P +  S  SS    
Sbjct: 287 APPQPAYTVSYNTMHPSTSHEASYYATPPPQYSYAYMHPGPWTEPPPSDFDSNPSS---- 346

Query: 302 EQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
                 VH                    PPS  F FFSDENP+ CS+M
Sbjct: 347 ------VH--------------------PPSDSFQFFSDENPNACSLM 350

[Close]

BLAST of Lsi06G001160 vs. TrEMBL
Match: A0A0B0NQ61_GOSAR (Copper-transporting ATPase 1 OS=Gossypium arboreum GN=F383_00918 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-17
Identity = 118/362 (32.60%), Postives = 155/362 (42.82%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTN--TDEQTLIKRLIKAGKHAEPWPEI---------KPISKNI 61
           GV+    D KQ K TV  N   + +TLIK+L+K G+HAE WPE          KP +K+ 
Sbjct: 62  GVYEAYADLKQQKATVKANLHVNAETLIKKLVKKGRHAELWPEKAESKEKKQGKPKNKDK 121

Query: 62  KEKQIAVE-IPAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDG 121
           +  Q   E    G     KQK++ + E   ++      +E   G ++N       +G   
Sbjct: 122 QGGQANGEGANNGNHGGDKQKETVKNEVKVQQEDGAKSSENGGGSSKN------GEGCSN 181

Query: 122 HGDATVTGGGGGPAERAVE----------------SSPSIAGETEPGVPSVAVAVEAS-- 181
              A   GGGG   +  V+                 + S   E + G        E +  
Sbjct: 182 VSKANDQGGGGAACKNGVQVKEPKTEVKQNVTPVAGNQSSVAEKKGGGSGAGGDTEGNGN 241

Query: 182 -----CSGGGEGRKKKKKKPQRKEKSGGASAGEVVSPQTVPTPASIGLATPLDHHHDQM- 241
                 SG G G KK K+K Q+     G   GE  S   VP            H    M 
Sbjct: 242 GNAGEKSGNGSGSKKNKRKGQKANTDEG---GEQHSGDAVPASIESHFKVHGPHGPVPMP 301

Query: 242 SSSNHSPPFNQP-YNYPVHTAVSQPAYVASYNTVYPSSTYGA-YYASPPPYSYAYVHSAA 301
           S +NHSPP   P Y YP +   + P Y+ SYNT YPSS+Y A YY SPPPYSYAY+H   
Sbjct: 302 SPANHSPPRQHPVYEYPTYYH-APPVYLTSYNTAYPSSSYTASYYTSPPPYSYAYMH--- 361

Query: 302 PRNISSLSSSSPVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCS 326
           P + +   +S   M  SY    S +SR                PS  F+ FSDENP+ CS
Sbjct: 362 PGHTTERQTSDMDMYSSYP---SYSSR----------------PSDSFEMFSDENPNACS 391

[Close]

BLAST of Lsi06G001160 vs. TAIR10
Match: AT1G56210.1 (AT1G56210.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 4.4e-13
Identity = 101/343 (29.45%), Postives = 147/343 (42.86%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEI--------KPIS---KNI 61
           GV  ++ID KQHKVTV      + L+K+L KAGK+AE  PEI        KP+    KN 
Sbjct: 66  GVFKVDIDVKQHKVTVIGIISPEILLKKLNKAGKNAEQLPEIPDPVDNKPKPVDPKEKNK 125

Query: 62  KEK-QIAVEIPAGETSAG----KQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISK 121
           K+K +  V+I    TS+G    ++  S E + P  E  V  K     G   +  V E  K
Sbjct: 126 KKKKEEKVQITNEATSSGIDNPEKPGSGECDKPESEKPVDEKCLSGDGGETSGPVKEEKK 185

Query: 122 GGDGHGDATVTGGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGGGEGRKKKKKK 181
                 D+        PA+ +  ++   A +T   VP             G+  KKKKKK
Sbjct: 186 DVLKEKDSGKEESPSPPADSSAPAAEKKAEDTGGAVPD-----------NGKVGKKKKKK 245

Query: 182 PQRKEKSGGASAGEVVSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHTA 241
            Q    +   + G   + Q++P P +     P++  +D   ++ ++PP +  Y YP    
Sbjct: 246 GQSLATTNNPTDGPART-QSLPPPTATDYDRPINQINDHHITTTNNPPRHDLYPYPAQGY 305

Query: 242 VS-QPAYVASYNTVYPSSTYGA--YYASPPPYSYAYVHSAAPRNISSLSSSSPVMEQSYA 301
            + Q  Y  SYN   P  +  A  YY  PPPYS                         YA
Sbjct: 306 YAPQVMYGVSYNVAQPPVSVDAASYYTPPPPYS-------------------------YA 364

Query: 302 HVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
           ++H+    +  +   P        PS  F+ FSDENP+GC+IM
Sbjct: 366 YMHNGYQPSDQNPCQPR-------PSDSFELFSDENPNGCAIM 364

[Close]

BLAST of Lsi06G001160 vs. TAIR10
Match: AT5G27690.1 (AT5G27690.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-09
Identity = 99/355 (27.89%), Postives = 133/355 (37.46%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKNIKEKQIAVEIPA 61
           GV+   ID KQ KVTV  N + + LIK+++KAG+HAE WP    +  NI           
Sbjct: 56  GVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAELWP--TSMENNI----------- 115

Query: 62  GETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVTGGGGG 121
                  QK+             P K+ E+   +E+    E +   +G GD  V GGGGG
Sbjct: 116 -NNDCNYQKK-------------PKKDNEETSGDEDDD--ENNNNNNGGGD--VGGGGGG 175

Query: 122 PAERAVESSPSIAGETEPGVPSVAVAVEASCSGGGEGRKKKKKKPQRKEKS--------- 181
                        G     V  V   V       G+G  KKKKK ++K+KS         
Sbjct: 176 ------------GGGNFDQVKQVVTFVNGQLQPQGDGAPKKKKKKKKKKKSLGNTTVVME 235

Query: 182 -GGASAGEVVSPQTVPTPASIGLATPLDHHHDQMSSSNHSPP----FNQPYNYPVHTAVS 241
            GG   G    PQ    P ++  + P DHH        H PP     +Q  N+P  T  S
Sbjct: 236 GGGGGGGGGGPPQNDGPPETVIYSAPQDHH------IIHGPPPHLHHHQQQNHPYPTVHS 295

Query: 242 QPAYVASYNTVYPSSTYGAYYASPPPYSYAYVHSAAP-----------------RNISSL 301
            P +       +P   YG     PPP  Y      AP                    +S 
Sbjct: 296 PPRH-------HPQQMYGPGPGLPPPSFYHTQPQTAPSYTVSYNTVHGPINNGGNETASY 352

Query: 302 SSSSPVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
            ++ P    SY     V +   S    PE     S PS  F+  S+ NP+ C +M
Sbjct: 356 YTAPPSHPTSYYSYEYVDTGYES--PPPEFYSYRSQPSESFESLSEGNPNSCCVM 352

[Close]

BLAST of Lsi06G001160 vs. NCBI nr
Match: gi|659099509|ref|XP_008450635.1| (PREDICTED: uncharacterized protein LOC103492164 [Cucumis melo])

HSP 1 Score: 427.2 bits (1097), Expect = 2.6e-116
Identity = 245/333 (73.57%), Postives = 268/333 (80.48%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKNIKEKQIAVEIPA 61
           GVH++EIDRKQ KVT+TTN DEQ+LIKRLIKAGKHAEPWPE KPISK   EKQIAVEIPA
Sbjct: 45  GVHSVEIDRKQQKVTITTNIDEQSLIKRLIKAGKHAEPWPETKPISKI--EKQIAVEIPA 104

Query: 62  GETSA-----GKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVT 121
           GETSA     GKQKQSTETEP AE LQVP +NEEK G NENVQV++ISK  DGHGDAT T
Sbjct: 105 GETSASVRDGGKQKQSTETEPSAEALQVPPRNEEKSGTNENVQVLQISKADDGHGDATET 164

Query: 122 GGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGGGEGRKKKKKKP--QRKEKSGG 181
               G  ER VES P IA ET+P +PS AV +EASCSG GEGRKKKKKK   QRKEKS G
Sbjct: 165 ---NGRVERVVESPPDIACETQPSMPSGAVDIEASCSGDGEGRKKKKKKKQGQRKEKSSG 224

Query: 182 ASAGEVVSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHTAVSQPAYVAS 241
           A AGE+VSP TVPTPA+IG   P     +Q+ SSNHSPPFN PYN+P+H A SQPAYVAS
Sbjct: 225 AVAGEMVSPPTVPTPANIGSPNP----PNQVPSSNHSPPFNNPYNHPLHVAASQPAYVAS 284

Query: 242 YNTVYPSSTYGAYYASPPPYSYAYVHSAAPRNISSLSSSSPVMEQSYAHVHSVASRNISS 301
           YNTVYP++T+ AYYASPP YSYAYVHS APRN ++LSSS P+ EQ Y + HSV  RNISS
Sbjct: 285 YNTVYPTNTHDAYYASPPSYSYAYVHSMAPRNSTTLSSSLPIAEQPYTYAHSVEPRNISS 344

Query: 302 LSS--PEVEQSNSPPSSPFDFFSDENPSGCSIM 326
           LSS  P VEQSNSPPSSPFDFFSDENPSGCSIM
Sbjct: 345 LSSLPPVVEQSNSPPSSPFDFFSDENPSGCSIM 368

[Close]

BLAST of Lsi06G001160 vs. NCBI nr
Match: gi|778662457|ref|XP_004135612.2| (PREDICTED: uncharacterized protein LOC101206264 [Cucumis sativus])

HSP 1 Score: 397.5 bits (1020), Expect = 2.2e-107
Identity = 231/335 (68.96%), Postives = 262/335 (78.21%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKNIKEKQIAVEIPA 61
           GVH++EIDRKQ KVT+TTN DEQ+LIKRLIKAG HAEPWPE KPISK IKEKQI VEIP 
Sbjct: 45  GVHSVEIDRKQQKVTITTNIDEQSLIKRLIKAGMHAEPWPETKPISKIIKEKQIPVEIPP 104

Query: 62  GETSA-----GKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVT 121
           GETSA     GK+KQ+TETEPPAEELQVP +NEEK G NENV+  +     DGHGDAT T
Sbjct: 105 GETSASVRDGGKKKQTTETEPPAEELQVPPRNEEKSGTNENVRRCD-----DGHGDATET 164

Query: 122 GGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGGGEGRKKKKKKP----QRKEKS 181
           GG   P ER VES P+I+ ET+PG+PS AV +EASCSG GE  +KKKKK     QRKEK+
Sbjct: 165 GG---PVERVVESPPNISSETQPGMPSGAVDIEASCSGDGEVMRKKKKKKKAQAQRKEKN 224

Query: 182 GGASAGEVVSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHTAVSQPAYV 241
            GA AGE+VSPQTVPTP +IG  TP     +Q+ SSNHSPPFN    +P+HT +SQPAY+
Sbjct: 225 SGAVAGEMVSPQTVPTPTNIGSPTP----PNQIPSSNHSPPFN----HPLHTTLSQPAYI 284

Query: 242 ASYNTVYPSSTYGAYYASPPPYSYAYVHSAAPRNISSLSSSSPVMEQSYAHVHSVASRNI 301
           ASYNT YP++T+ AYYASPP YSYAYVHS AP    +LSSS P+MEQ YA+ HS+  RNI
Sbjct: 285 ASYNTAYPTNTHDAYYASPPSYSYAYVHSMAP----TLSSSLPIMEQPYAYAHSIEPRNI 344

Query: 302 SSLSS--PEVEQSNSPPSSPFDFFSDENPSGCSIM 326
           S LSS  P VEQSNSPPSSPFDFFSDENPSGCSIM
Sbjct: 345 SPLSSLPPVVEQSNSPPSSPFDFFSDENPSGCSIM 359

[Close]

BLAST of Lsi06G001160 vs. NCBI nr
Match: gi|147768217|emb|CAN73618.1| (hypothetical protein VITISV_004114 [Vitis vinifera])

HSP 1 Score: 116.3 bits (290), Expect = 9.9e-23
Identity = 124/351 (35.33%), Postives = 159/351 (45.30%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEI-----KPISKNIKEKQIA 61
           GV+  EID +Q KV VT N D +TL+K+L+K GKHAE WPE      +  SKN KEKQ  
Sbjct: 47  GVYTTEIDTRQQKVCVTGNVDVETLLKKLVKNGKHAELWPEKADHQKEKKSKN-KEKQKD 106

Query: 62  VEIPAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVT 121
            E    E   G  K+ +  +   E +Q P+KN E    +        S GG G G  T  
Sbjct: 107 QESNEQEGGHGGDKEKSTVK--FEAVQEPTKNPEPK--HSEAAAASGSGGGGGGGGGTKV 166

Query: 122 GGGGGPAERAV----ESSPSI-------AGETEPGVPSVAVAVEASCS--GGGEGRKKKK 181
           G  G   +       E+ P +       AGE  P         +A     G G G KKKK
Sbjct: 167 GENGAAVKNGAAAQKEAKPEVKKPEASPAGEAPPAAEKKVGESDACADKGGSGNGSKKKK 226

Query: 182 KKPQRKEKSGGASAGEV-VSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPV 241
           KK Q+     GA + +   +P   P P+      P   H    +S+         Y+YP 
Sbjct: 227 KKGQKPNSDVGAPSSDTPAAPPGSPNPS------PAPSHGPAPASTQ------TVYHYPP 286

Query: 242 HT-AVSQPAYVASYNTVYPSSTYGA-YYASPPP-YSYAYVHSAA-----PRNISSLSSSS 301
           H  A  QPAY  SYNT++PS+++GA YYA+PPP YSYAY+H        P +  S  SS 
Sbjct: 287 HYYAPPQPAYTVSYNTMHPSTSHGASYYATPPPQYSYAYMHPGPWTEPPPSDFDSNPSS- 346

Query: 302 PVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
                    VH                    PPS  F FFSDENP+ CS+M
Sbjct: 347 ---------VH--------------------PPSDSFQFFSDENPNACSLM 350

[Close]

BLAST of Lsi06G001160 vs. NCBI nr
Match: gi|802756377|ref|XP_012089017.1| (PREDICTED: uncharacterized protein LOC105647507 [Jatropha curcas])

HSP 1 Score: 113.6 bits (283), Expect = 6.4e-22
Identity = 122/352 (34.66%), Postives = 155/352 (44.03%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEI-----KPISKNI-----K 61
           GV+  +ID KQ KVTV  N D  TLIK+L+KAGKHAE WPE      K  SKN      K
Sbjct: 62  GVYKTDIDLKQQKVTVLGNVDADTLIKKLVKAGKHAELWPEKADHKKKGKSKNKDKEKEK 121

Query: 62  EKQIAVEIPAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVI--------EI 121
           EKQ + +  +GE    K K++ +TE     +Q PSK  E    N+N             +
Sbjct: 122 EKQQSDQ-ESGEEGGEKNKETVKTEVLI--VQDPSKPNENGTANKNGGTTTKNGAVESNV 181

Query: 122 SKGGDGHGDATVTGGGGGPAERAVESSPSIAGETEPGVPSVAVAVEASCSGG----GEGR 181
             G      AT   GGG   E  +E    +        P     + +   GG    G G 
Sbjct: 182 DVGKSKEAGATSKTGGGQVKEVKIEMKQPVTCPAGTQSPVTEKKIGSENEGGAEKSGNGG 241

Query: 182 KKKKKKPQRKEKSGGASAGEVVSPQTVPTPASIGLATPLDHHHDQMS------SSNHSPP 241
           KKKKKK     K    +AGE      VP     G  +P+ H H Q        S+NHS P
Sbjct: 242 KKKKKKGH---KGNNNNAGEGEHSSDVPA----GTGSPI-HGHGQGPIGPVPYSTNHSLP 301

Query: 242 FNQPYNYPVHTAVSQPAYVASYNTVYPSSTYGAYYASPPPYSYAYVHSAAPRNISSLSSS 301
            +  Y YP H     PAY  +YNT YPS++YGA   +PP YSYAY+           +S 
Sbjct: 302 HHHVYQYPSHYYTPPPAYAVNYNTAYPSTSYGA-SINPPSYSYAYMQ-------PGFASE 361

Query: 302 SPVMEQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
            P +                     + E + S P   F+ FSDENP+ CSIM
Sbjct: 362 PPAL---------------------DFESNPSQPLDSFEIFSDENPNACSIM 373

[Close]

BLAST of Lsi06G001160 vs. NCBI nr
Match: gi|225450759|ref|XP_002279364.1| (PREDICTED: brain acid soluble protein 1 [Vitis vinifera])

HSP 1 Score: 110.5 bits (275), Expect = 5.4e-21
Identity = 116/348 (33.33%), Postives = 152/348 (43.68%), Query Frame = 1


Query: 2   GVHAIEIDRKQHKVTVTTNTDEQTLIKRLIKAGKHAEPWPEIKPISKN--IKEKQIAVEI 61
           GV+  EID +Q KV VT N D +TL+K+L+K GKHAE WPE     K    K K+   + 
Sbjct: 47  GVYTTEIDTRQQKVCVTGNVDVETLLKKLVKNGKHAELWPEKADHQKEKKSKNKEKQKDQ 106

Query: 62  PAGETSAGKQKQSTETEPPAEELQVPSKNEEKCGINENVQVIEISKGGDGHGDATVTGGG 121
            + E   G      ++    E +Q P+KN E    +        S GG G G     G  
Sbjct: 107 ESNEQEGGHDGDKEKSTVKFEAVQEPTKNPEPK--HSEAAAASGSGGGGGGGGGAKVGEN 166

Query: 122 GGPAERAV----ESSPSI-------AGETEPGVPSVAVAVEASCS--GGGEGRKKKKKKP 181
           G   +       E+ P +       AGE  P         +A     G G G KKKKKK 
Sbjct: 167 GAAVKNGAAAQKEAKPEVKKPEASPAGEAPPAAEKKVGESDACADKGGSGNGSKKKKKKG 226

Query: 182 QRKEKSGGASAGEV-VSPQTVPTPASIGLATPLDHHHDQMSSSNHSPPFNQPYNYPVHT- 241
           Q+     GA + +   +P   P P+      P   H    +S+         Y+YP H  
Sbjct: 227 QKPNSDVGAPSSDTPAAPPGSPNPS------PAPSHGPAPASTQ------TVYHYPPHYY 286

Query: 242 AVSQPAYVASYNTVYPSSTYGA-YYASPPP-YSYAYVHSAA-----PRNISSLSSSSPVM 301
           A  QPAY  SYNT++PS+++ A YYA+PPP YSYAY+H        P +  S  SS    
Sbjct: 287 APPQPAYTVSYNTMHPSTSHEASYYATPPPQYSYAYMHPGPWTEPPPSDFDSNPSS---- 346

Query: 302 EQSYAHVHSVASRNISSLSSPEVEQSNSPPSSPFDFFSDENPSGCSIM 326
                 VH                    PPS  F FFSDENP+ CS+M
Sbjct: 347 ------VH--------------------PPSDSFQFFSDENPNACSLM 350

The following BLAST results are available for this feature:

BLAST of Lsi06G001160 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi06G001160 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LZ09_CUCSA	1.5e-107	68.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G569440 PE=4 SV=1	[more]
A5AP55_VITVI	6.9e-23	35.33	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_004114 PE=4 SV=1	[more]
A0A067JKX9_JATCU	4.5e-22	34.66	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23316 PE=4 SV=1	[more]
F6I4N0_VITVI	3.8e-21	33.33	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0060g02090 PE=4 SV=...	[more]
A0A0B0NQ61_GOSAR	2.5e-17	32.60	Copper-transporting ATPase 1 OS=Gossypium arboreum GN=F383_00918 PE=4 SV=1	[more]

BLAST of Lsi06G001160 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G56210.1	4.4e-13	29.45	Heavy metal transport/detoxification superfamily protein	[more]
AT5G27690.1	1.7e-09	27.89	Heavy metal transport/detoxification superfamily protein	[more]

BLAST of Lsi06G001160 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659099509\|ref\|XP_008450635.1\|	2.6e-116	73.57	PREDICTED: uncharacterized protein LOC103492164 [Cucumis melo]	[more]
gi\|778662457\|ref\|XP_004135612.2\|	2.2e-107	68.96	PREDICTED: uncharacterized protein LOC101206264 [Cucumis sativus]	[more]
gi\|147768217\|emb\|CAN73618.1\|	9.9e-23	35.33	hypothetical protein VITISV_004114 [Vitis vinifera]	[more]
gi\|802756377\|ref\|XP_012089017.1\|	6.4e-22	34.66	PREDICTED: uncharacterized protein LOC105647507 [Jatropha curcas]	[more]
gi\|225450759\|ref\|XP_002279364.1\|	5.4e-21	33.33	PREDICTED: brain acid soluble protein 1 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046916	cellular transition metal ion homeostasis
biological_process	GO:0030001	metal ion transport
biological_process	GO:0008150	biological_process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046914	transition metal ion binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G001160.1	Lsi06G001160.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 2..41 score: 3.
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 2..74 score: 1.2E-37coord: 154..242 score: 1.2E-37coord: 277..324 score: 1.2
None	No IPR available	PANTHER	PTHR22814:SF75	HEAVY METAL TRANSPORT/DETOXIFICATION DOMAIN-CONTAINING PROTEIN	coord: 2..74 score: 1.2E-37coord: 154..242 score: 1.2E-37coord: 277..324 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi06G001160	Csa1G569440	Cucumber (Chinese Long) v2	culsiB058
Lsi06G001160	CSPI01G26500	Wild cucumber (PI 183967)	cpilsiB059
Lsi06G001160	Cucsa.181950	Cucumber (Gy14) v1	cgylsiB304
Lsi06G001160	CmaCh11G011360	Cucurbita maxima (Rimu)	cmalsiB146
Lsi06G001160	CmoCh11G011780	Cucurbita moschata (Rifu)	cmolsiB130
Lsi06G001160	Cla005223	Watermelon (97103) v1	lsiwmB396
Lsi06G001160	MELO3C015311	Melon (DHL92) v3.5.1	lsimeB396
Lsi06G001160	ClCG03G017100	Watermelon (Charleston Gray)	lsiwcgB364
Lsi06G001160	MELO3C015311.2	Melon (DHL92) v3.6.1	lsimedB418
Lsi06G001160	Cla97C03G067440	Watermelon (97103) v2	lsiwmbB365
Lsi06G001160	Bhi02G001755	Wax gourd	lsiwgoB528
Lsi06G001160	Cp4.1LG04g00530	Cucurbita pepo (Zucchini)	cpelsiB564
Lsi06G001160	CsGy1G025740	Cucumber (Gy14) v2	cgyblsiB055
Lsi06G001160	Carg25030	Silver-seed gourd	carlsiB221

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi06G001160	Bottle gourd (USVL1VR-Ls)	lsilsiB060
Lsi06G001160	Bottle gourd (USVL1VR-Ls)	lsilsiB161
Lsi06G001160	Cucumber (Gy14) v1	cgylsiB101
Lsi06G001160	Cucurbita maxima (Rimu)	cmalsiB076
Lsi06G001160	Cucurbita maxima (Rimu)	cmalsiB178
Lsi06G001160	Cucurbita maxima (Rimu)	cmalsiB734
Lsi06G001160	Cucurbita moschata (Rifu)	cmolsiB062
Lsi06G001160	Cucurbita moschata (Rifu)	cmolsiB168
Lsi06G001160	Cucurbita moschata (Rifu)	cmolsiB723
Lsi06G001160	Cucurbita pepo (Zucchini)	cpelsiB095
Lsi06G001160	Cucurbita pepo (Zucchini)	cpelsiB302
Lsi06G001160	Cucurbita pepo (Zucchini)	cpelsiB682
Lsi06G001160	Wild cucumber (PI 183967)	cpilsiB134
Lsi06G001160	Cucumber (Chinese Long) v2	culsiB136
Lsi06G001160	Melon (DHL92) v3.5.1	lsimeB416
Lsi06G001160	Watermelon (Charleston Gray)	lsiwcgB359
Lsi06G001160	Watermelon (97103) v1	lsiwmB409
Lsi06G001160	Cucumber (Chinese Long) v3	cuclsiB068
Lsi06G001160	Wax gourd	lsiwgoB486
Lsi06G001160	Wax gourd	lsiwgoB507