Lsi05G018500 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G018500
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Alkaline ceramidase 3
Location	chr05 : 25744698 .. 25747533 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAACACGCGGCATTAAGGCGCTTCAAAGTCGGATTCTGATTCCATTAATCACGAGCCGCGCCAAAGCTCGATGACCGGAGCAATATCAACCGTCGGTGTATATAATCTAACGGCCACAAATTGATTTCCGTCATCTTCCCTTATATCTTCATCACGCGCTCGTCGTCCTCCTTCTGGGTAGCCCTAATTGGTTCGGACTCTCAGCTCAGATCTCCTCCGATCGCTTCGCACCACCGACAGAAAGACCTCAGGAATTTGAATTTCAAGGATCCAGCAATGGTTCTTCTCTAGCTTAGATCTGTTTCTGAAGGTTAGTAGTTGATTCAATATTTTTTTTGCTCTCTTTCAATTTTGATTTTTGTTCTATATGTTTAGTTGTATTGCTCCGTGATTGTTGTGTATGGAGATTCGAACTGTTTCGTCTGTTTCATAAACTTCTCGAGGAAGTCAAGTCATGATGAAAAAATGGAAGAAATAAAAAAAGTTGCCATGGTCAGCGTAGTTTTTTTTTTTTTTGGTTAAAATTTGCTTCAGAAAACGTTGCGATGGTTATTAGTGTCGTTGTGTTCGAGTTAGTTTACTCTTGACGTCTGTTATATGCACGGTAAAGAATGTAAAGAATGTTAAGGAATGATGTATGAAAGTTGCAATTACGTTTTCATAGCTTAGTGCGATTCTAGGTTTGGCGGTTTCTGTTATTGTAAGTAAAGTACGAAACATGGAGTTGGTTATTGATATTGTAGTTTCGGGATTGTTAACTTGGCCAAGGAGTTCAGGAAGATGGAGAGTCTCTTTCTGTCTAGACATTTCACATCTACGGGTTGTTTTCGTTAATGAAATTGGTCAATTAATCCTTTAGTTCTAAGAATCCATGATAATTCAGACAAGTTTCCTCATCAAATACATATTTCCTCAAGATATTCCAGTATTTGCTTGGGTAAAAAATGGAAGTTGTGTTTACTTTGACTTGTATATGTGTTATTTTTTGCACTGATTTTTTACTTATTAAAGAATATGATTACAGGTTTGTAAATTTTGAACCATCCTAGCAGGCAATTCAAGTGTTGTCGGGTATACTTAATTGTTGAATGGCAGATACAATATCTAGTTTCTGGGGTCCTGTCACATCTCCTGAGTGGTGTGAAAAGAATTATGTCTACTCTTCTTATATTGCCGAGTTCTTCAACACGGTATCAAATATTCCATGCCTCATTTTAGCACTTATTGGTCTCGTAAATGCTTTGAGACAAAGGTTTGAGAAGAGGTTTAGTGTTCTTCACATATCAAATATGATCCTCGCCATAGGAAGCATGTTGTATCATGCCACCTTGCAACGAGTGTAAGTCTTCTTCCTTTATATTGAAGAATAAAAGAATGAAGATGACTATGTGGCAGTCCCTTTTTTTTGGCATGGAATTTCTTCATAATTGTTTGAGTTGAACTTTTAAATTTCTATGTAGAGGCTGTAATTTGTAAATCAAAAAGTCTTTAAGCTACCGATATAATATAGATTTTACAAGCGATCAAATTACAAAATGTTGTGTGGAGTTGAGGGACAATATATTTTGGATGTGTATAAGGAAAATATGGTGAACATAGCTTTGTCCTTGTCCATGTCATTATATATTTCTTTTCGGAGACCTTTCGGCTAAAGGAAAGATAGGAGGAAACTGCCAAGCAAATGATTAACTAGACTTATTGGGAAACTTTAGTTTGTTTTTTCAACTGCCCAACAAAAATTTGGACTATGCTGTTTGGTTTGCTTTGTGCTTACACGTCGGGATGGGAAAAAGATTGATTTAGCCATTTAGGCTTACAAGAAACTTAAAAATAATTGTATCCAGTCTGTAGAAGCCGTAGTTTCTTAAAGTAATGGAGTTTGTTTATGTCTATGAACTTATTATAGCTTATTCACACACACACAAGAAAAGAAAAAAAGAGAAAAGAAAAAAAAAATTTAGAACTGTCTCATAATTTCATCAATTATTGGAATTCATTCCCTCATGGATGAATTTTCTGGTTCTTCTATCACCTCTGATTTTCTATTCTTCCAATGCGTTAGGAGTTGCAACTTTTTACATTTTCTTCCCCCTCTAGCCGAGGTTTGGTACATGTAATGATGAGTGCCCTGTGAAATTATTTTTTTCTGGAACTTTAATGGTTTATTTCATTAATAGATGAACGTTAACTATAGCTAGTGGATGATAACTTATTATGCCATGAAATTTCTGAATGTTTTATACAACACAGGCTTGCATGTTCTGTTAAATCTTCATCCACTATAATATCTATTGGATTAAACGATAAATTTAATTTCAGACAACAGCAAGGTGATGAAACACCAATGGTATGGGAAATGCTCCTCTACATCTACATCCTTTATTCACCAGATTGGCACTACCGGAGTACGATGCCTACGTTCTTGTTTCTATATGGAGCTGCCTTTGCTGTTGCCCATGCAATCATACGTTTTGGAGCAGGCTTTAAGGTACATTATGCAATACTATGTCTTCTCTGCATCCCTCGAATGTACAAGTACTATATTCACACGAACGACATACACGCGAAGCGGCTTGCAAAGTTATACTTGGCAACCATCTGTCTTGGGACCGTTTGCTGGCTTTTTGATCGCTTGTACTGCAGGAACATCTCTAGCTGGTATTTCAACCCTCAGGGTCATGCATTATGGCATGTATTAATGGGATTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTAGAGTGGAACCCGAAAGTAGTCCACTTTTTGGGGTTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGA

mRNA sequence

AAATAACACGCGGCATTAAGGCGCTTCAAAGTCGGATTCTGATTCCATTAATCACGAGCCGCGCCAAAGCTCGATGACCGGAGCAATATCAACCGTCGGTGTATATAATCTAACGGCCACAAATTGATTTCCGTCATCTTCCCTTATATCTTCATCACGCGCTCGTCGTCCTCCTTCTGGGTAGCCCTAATTGGTTCGGACTCTCAGCTCAGATCTCCTCCGATCGCTTCGCACCACCGACAGAAAGACCTCAGGAATTTGAATTTCAAGGATCCAGCAATGGTTCTTCTCTAGCTTAGATCTGTTTCTGAAGGTTTGTAAATTTTGAACCATCCTAGCAGGCAATTCAAGTGTTGTCGGGTATACTTAATTGTTGAATGGCAGATACAATATCTAGTTTCTGGGGTCCTGTCACATCTCCTGAGTGGTGTGAAAAGAATTATGTCTACTCTTCTTATATTGCCGAGTTCTTCAACACGGTATCAAATATTCCATGCCTCATTTTAGCACTTATTGGTCTCGTAAATGCTTTGAGACAAAGGTTTGAGAAGAGGTTTAGTGTTCTTCACATATCAAATATGATCCTCGCCATAGGAAGCATGTTGTATCATGCCACCTTGCAACGAGTGCTTGCATGTTCTGTTAAATCTTCATCCACTATAATATCTATTGGATTAAACGATAAATTTAATTTCAGACAACAGCAAGGTGATGAAACACCAATGGTATGGGAAATGCTCCTCTACATCTACATCCTTTATTCACCAGATTGGCACTACCGGAGTACGATGCCTACGTTCTTGTTTCTATATGGAGCTGCCTTTGCTGTTGCCCATGCAATCATACGTTTTGGAGCAGGCTTTAAGGTACATTATGCAATACTATGTCTTCTCTGCATCCCTCGAATGTACAAGTACTATATTCACACGAACGACATACACGCGAAGCGGCTTGCAAAGTTATACTTGGCAACCATCTGTCTTGGGACCGTTTGCTGGCTTTTTGATCGCTTGTACTGCAGGAACATCTCTAGCTGGTATTTCAACCCTCAGGGTCATGCATTATGGCATGTATTAATGGGATTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTAGAGTGGAACCCGAAAGTAGTCCACTTTTTGGGGTTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGA

Coding sequence (CDS)

ATGGCAGATACAATATCTAGTTTCTGGGGTCCTGTCACATCTCCTGAGTGGTGTGAAAAGAATTATGTCTACTCTTCTTATATTGCCGAGTTCTTCAACACGGTATCAAATATTCCATGCCTCATTTTAGCACTTATTGGTCTCGTAAATGCTTTGAGACAAAGGTTTGAGAAGAGGTTTAGTGTTCTTCACATATCAAATATGATCCTCGCCATAGGAAGCATGTTGTATCATGCCACCTTGCAACGAGTGCTTGCATGTTCTGTTAAATCTTCATCCACTATAATATCTATTGGATTAAACGATAAATTTAATTTCAGACAACAGCAAGGTGATGAAACACCAATGGTATGGGAAATGCTCCTCTACATCTACATCCTTTATTCACCAGATTGGCACTACCGGAGTACGATGCCTACGTTCTTGTTTCTATATGGAGCTGCCTTTGCTGTTGCCCATGCAATCATACGTTTTGGAGCAGGCTTTAAGGTACATTATGCAATACTATGTCTTCTCTGCATCCCTCGAATGTACAAGTACTATATTCACACGAACGACATACACGCGAAGCGGCTTGCAAAGTTATACTTGGCAACCATCTGTCTTGGGACCGTTTGCTGGCTTTTTGATCGCTTGTACTGCAGGAACATCTCTAGCTGGTATTTCAACCCTCAGGGTCATGCATTATGGCATGTATTAATGGGATTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTAGAGTGGAACCCGAAAGTAGTCCACTTTTTGGGGTTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGA

Protein sequence

MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRFSVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYFANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ

Homology

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BLAST of Lsi05G018500 vs. Swiss-Prot
Match: ACER3_MOUSE (Alkaline ceramidase 3 OS=Mus musculus GN=Acer3 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 4.5e-24
Identity = 74/281 (26.33%), Postives = 127/281 (45.20%), Query Frame = 1


Query: 8   FWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRFSVLHIS 67
           +WGP TS  +WCE+NYV + ++AEF+NTVSN+  +I  + G +  +R R EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLA 69

Query: 68  NMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEMLLYIYI 127
             ++ +GS  +H TL+                       +  Q  DE PM++   +++Y 
Sbjct: 70  LTVVGMGSWCFHMTLK-----------------------YEMQLLDELPMIYSCCIFVYC 129

Query: 128 LY-------SPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYK 187
           ++       S ++H   T    LFLY       +  ++     +V Y +L    + R   
Sbjct: 130 MFECFKTKSSINYHLLFT----LFLYSLTVTTIYLKVKEPIFHQVMYGMLVFTLVLRSI- 189

Query: 188 YYIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWY--------FNPQGHALWH 247
           Y +       + L    L    LG + W  D ++C ++ ++            Q HA WH
Sbjct: 190 YIVTWVYPWLRGLGYTSLTVFLLGFLLWNIDNIFCDSLRNFRKRVPPVLGVTTQFHAWWH 249

Query: 248 VLMGFNSYFANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQ 273
           +L G  SY    F ++ R   L + PKV    G++P V  +
Sbjct: 250 ILTGLGSYLHILFSLYTRTLYLRYRPKVKFLFGIWPAVMFE 262

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BLAST of Lsi05G018500 vs. Swiss-Prot
Match: ACER3_HUMAN (Alkaline ceramidase 3 OS=Homo sapiens GN=ACER3 PE=1 SV=3)

HSP 1 Score: 105.9 bits (263), Expect = 7.1e-22
Identity = 72/278 (25.90%), Postives = 124/278 (44.60%), Query Frame = 1


Query: 8   FWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRFSVLHIS 67
           +WGP TS  +WCE+NY  + YIAEF+NTVSN+  +I  + G V ++R   EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLA 69

Query: 68  NMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEMLLYIYI 127
             ++ +GS  +H TL+                       +  Q  DE PM++   +++Y 
Sbjct: 70  LTVVGMGSWCFHMTLK-----------------------YEMQLLDELPMIYSCCIFVYC 129

Query: 128 LY-------SPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYK 187
           ++       S ++H   T    L L+       +  ++     +V Y +L    + R   
Sbjct: 130 MFECFKIKNSVNYHLLFT----LVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSI- 189

Query: 188 YYIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWY--------FNPQGHALWH 247
           Y +       + L    L    LG + W  D ++C ++ ++            Q HA WH
Sbjct: 190 YIVTWVYPWLRGLGYTSLGIFLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWH 249

Query: 248 VLMGFNSYFANTFLMFCRAQQLEWNPKVVHFLGLFPYV 270
           +L G  SY    F ++ R   L + PKV    G++P +
Sbjct: 250 ILTGLGSYLHILFSLYTRTLYLRYRPKVKFLFGIWPVI 259

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BLAST of Lsi05G018500 vs. Swiss-Prot
Match: YDC1_YEAST (Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YDC1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.0e-15
Identity = 80/301 (26.58%), Postives = 128/301 (42.52%), Query Frame = 1


Query: 5   ISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRFSVL 64
           I  +WG  TS  +WCE+NYV S YIAE+ NT++N   L+ A     +A R + E R+ ++
Sbjct: 12  IEGYWGKPTSLIDWCEENYVVSPYIAEWSNTITNSIFLMTAFYSTYSAWRNKLETRYILI 71

Query: 65  HISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEMLLY 124
            +   ++ IGS L+H TLQ                       +R Q  DE PM++  ++ 
Sbjct: 72  GMGFSLVGIGSWLFHMTLQ-----------------------YRYQLLDELPMLYATIIP 131

Query: 125 IYILYS-------------PDWHYRSTMPTFLFLYGAAFAVA--HAIIRFGAGFKVHYAI 184
            + +++              +  +R  M     + G    +   + +++  A F+V Y I
Sbjct: 132 SWSIFAETQEILIKDEKKRKESSFRIQMVISFIMCGIVTILTWIYVVVQKPAIFQVLYGI 191

Query: 185 LCLLCIPRM-YKYYIHTNDIHAKRLAKLYLATI------CLGTVCWLFDRLYCRNISSWY 244
           L LL +    +  Y H +D  AK+   L++  +       +G +CW  D   C   S W 
Sbjct: 192 LTLLVVVLSGWLTYYHVHDSFAKK--NLFITMVMGMIPFVIGFICWQLDIHLC---SFWI 251

Query: 245 F-------NPQG-----HALWHVLMGFNSYFANTFLMFCRAQQLEWNPKVVHFL---GLF 268
           +        P G     HA WH+L G   Y    +L + R      NP    F+   G F
Sbjct: 252 YIRRTYLALPLGVLLELHAWWHLLTGTGVYIFVVYLQYLRI-LTHGNPNDFLFIWRWGFF 283

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BLAST of Lsi05G018500 vs. Swiss-Prot
Match: DCD3A_DICDI (Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum GN=dcd3A PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.2e-13
Identity = 71/294 (24.15%), Postives = 119/294 (40.48%), Query Frame = 1


Query: 11  PVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGL---------------VNALRQR 70
           P  S +WCE NY YS YIAEF+NT S++   +  + G+               +  L+Q 
Sbjct: 13  PTASIDWCELNYTYSPYIAEFYNTFSSLIISLFGIYGIWIMMPNFGTGVEKEHIKILKQL 72

Query: 71  FEKRFSVL-HISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDET 130
             +   +L +IS +++ +GS  YHATL                        ++ Q  DE 
Sbjct: 73  DVRNKVILSYISLIVVGVGSAFYHATLL-----------------------YQNQLFDEL 132

Query: 131 PMVWEMLLYIYILYSPDWH---------------YRSTMPTFLFLYGAAFAVA------- 190
           PM++  L+ +YI+ +                    R  +P  L  YG    +        
Sbjct: 133 PMIYTALIMLYIMVTVGEEKTKKGFKGGVLGNSLLRHLLPYLLIAYGLFVTITILVIQDQ 192

Query: 191 HAIIRFGAGFKVHYAIL-CLLCIPRMYKYYIHTNDIHAKRLAKLYLATICLGTVCWLFDR 250
             I++   G  V Y +   +  I +     + +N      L K    ++ +G  CW+ +R
Sbjct: 193 PKILQVSFGALVFYVVFHSIYLINKKKPDGMPSNP--DSYLYKYAFVSMLVGFTCWVVER 252

Query: 251 LYCRNISSWYFNPQGHALWHVLMGFNSYFANTFLMFCRAQQLEWNPKVVHFLGL 266
            +C+N  ++ F  Q HA WH   G ++Y    FL+    +   +   + H LGL
Sbjct: 253 YFCKNGKTFGF--QLHAFWHFFTGMSTYVWTQFLICKLLEAKHYCVGIKHTLGL 279

[Close]

BLAST of Lsi05G018500 vs. Swiss-Prot
Match: DCD3B_DICDI (Putative alkaline ceramidase dcd3B OS=Dictyostelium discoideum GN=dcd3B PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-11
Identity = 74/297 (24.92%), Postives = 121/297 (40.74%), Query Frame = 1


Query: 7   SFWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILALIG------------------ 66
           ++WG   SP +WCE+NY+ S YI EF+NT S+       + G                  
Sbjct: 6   NYWGVPDSPIDWCEENYIISKYICEFYNTFSSFIITAFGVYGIFLMMSASSRDQALFQHV 65

Query: 67  -LVNALRQRFEKRFSVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNF 126
            ++  L+ R +  FS L ++  I+ +GS  YHATL                        +
Sbjct: 66  KIMKELKIRQKVLFSYLSLA--IVGVGSAFYHATLL-----------------------Y 125

Query: 127 RQQQGDETPMVWEMLLYIYILYSPD-----------WHYRSTMPTFLFLYGAAFAVAHAI 186
           + Q  DE PM+    +++Y + + D              R  +P  L LY    A+   I
Sbjct: 126 KNQLFDEFPMMLTASMFVYCILTIDPVDEKNDTATYKLMRRFLPYILSLYVIVVAITITI 185

Query: 187 IRFGAGFKVHYAILCLLCIPRMYKYYIHTN--------DIHAKRLAKLYLATICLGTVCW 246
           IR      +  +   LL    ++  Y++T+        + + K+   L +A++ +  + W
Sbjct: 186 IRDSP--IILQSSFGLLIFSNVFLSYMYTSRCLKTPVMESNPKKFLYLCIASMGIAYISW 245

Query: 247 LFDRLYCRNISSWYFNP--QGHALWHVLMGF-NSYFANTFLMFCRAQ-----QLEWN 257
           L +R  C N    Y  P  Q HA+WH L G    Y+   F+  C  +     +L WN
Sbjct: 246 LTERKLCNN---GYVIPGVQLHAVWHALTGLAGFYYIQFFITSCLEKHGYKTKLNWN 272

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BLAST of Lsi05G018500 vs. TrEMBL
Match: A0A0A0L9D8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G130320 PE=4 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 6.3e-142
Identity = 247/277 (89.17%), Postives = 251/277 (90.61%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLHISNM+LAIGSMLYHATLQRV                       QQQGDETPMVWEM
Sbjct: 61  SVLHISNMVLAIGSMLYHATLQRV-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY
Sbjct: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YIHTND+HAKRLAKLYL TI LGTVCWLFDRLYCR ISSWYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIHTNDVHAKRLAKLYLTTISLGTVCWLFDRLYCRKISSWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQQLEWNP+VVHFLGLFPYVK+QKPKSQ
Sbjct: 241 ANTFLMFCRAQQLEWNPRVVHFLGLFPYVKVQKPKSQ 254

[Close]

BLAST of Lsi05G018500 vs. TrEMBL
Match: A0A0D2UTV4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G156200 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.3e-126
Identity = 214/277 (77.26%), Postives = 236/277 (85.20%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           M   +SSFWGPVT+ EWCEKNYVYSSYIAEFFNT+SN+PC++LALIGL+NALRQRFEKRF
Sbjct: 1   MEGGLSSFWGPVTAAEWCEKNYVYSSYIAEFFNTISNVPCILLALIGLINALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLH+SNMILAIGSMLYHATLQ+                       +QQQGDETPMVWEM
Sbjct: 61  SVLHVSNMILAIGSMLYHATLQQC----------------------KQQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLY YILYSPDWHYRSTMPTFLFLYG  FA+AHA++RFG GFKVHY ILCLLCIPRMYKY
Sbjct: 121 LLYFYILYSPDWHYRSTMPTFLFLYGVGFAMAHALLRFGIGFKVHYVILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YI+T D  AKRLAKLY+AT+ LG++CWL DRL+C+ IS WYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIYTEDASAKRLAKLYVATLFLGSICWLADRLFCKEISGWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQ+ EW+PKVVHFLG FPYVKIQKPKSQ
Sbjct: 241 ANTFLMFCRAQRREWDPKVVHFLGFFPYVKIQKPKSQ 255

[Close]

BLAST of Lsi05G018500 vs. TrEMBL
Match: A0A0B0NPT2_GOSAR (Alkaline ceramidase 3 OS=Gossypium arboreum GN=F383_18020 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.3e-126
Identity = 214/277 (77.26%), Postives = 235/277 (84.84%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           M   +SSFWGPVT+ EWCEKNYVYSSYIAEFFNT+SN+PC++LALIGL+NALRQRFEKRF
Sbjct: 1   MEGGLSSFWGPVTAAEWCEKNYVYSSYIAEFFNTISNVPCILLALIGLINALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLH+SNMILAIGSMLYH+TLQ +                       QQQGDETPMVWEM
Sbjct: 61  SVLHVSNMILAIGSMLYHSTLQHM-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLY YILYSPDWHYRSTMPTFLFLYG  FA+ HA++RFG GFKVHY ILCLLCIPRMYKY
Sbjct: 121 LLYFYILYSPDWHYRSTMPTFLFLYGVGFAIVHALLRFGIGFKVHYVILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YI+T D  AKRLAKLY+AT+ LG++CWL DRL+C+ ISSWYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIYTEDASAKRLAKLYVATLFLGSICWLADRLFCKEISSWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQQ EW+PKVVHFLG FPYVKIQKPKSQ
Sbjct: 241 ANTFLMFCRAQQREWDPKVVHFLGFFPYVKIQKPKSQ 254

[Close]

BLAST of Lsi05G018500 vs. TrEMBL
Match: U5FNC0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s08440g PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 9.1e-125
Identity = 214/277 (77.26%), Postives = 233/277 (84.12%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MA+ ISSFWGPVTS EWCEKNYVYSSYIAEFFNT+SNIP ++LAL GL+NA RQRFEKRF
Sbjct: 1   MAEGISSFWGPVTSAEWCEKNYVYSSYIAEFFNTISNIPGILLALAGLINAFRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           S+LHISNMILAIGSMLYHATLQ +                       QQQGDETPMVWEM
Sbjct: 61  SILHISNMILAIGSMLYHATLQHM-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLYIYILYSPDWHYRSTMPTFLF YGAAFA+ HA++R   GFKVHY ILCLLC+PRMYKY
Sbjct: 121 LLYIYILYSPDWHYRSTMPTFLFFYGAAFAIVHALVRLRMGFKVHYVILCLLCVPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YI+T D  AKRLAKLYLATI +G++CWLFDRL+C NIS WYFNP+GHALWHVLMGFNSYF
Sbjct: 181 YIYTKDASAKRLAKLYLATIFMGSLCWLFDRLFCNNISRWYFNPEGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQQL WNPKV HF+G FPYVKIQKPK+Q
Sbjct: 241 ANTFLMFCRAQQLGWNPKVAHFMGFFPYVKIQKPKTQ 254

[Close]

BLAST of Lsi05G018500 vs. TrEMBL
Match: B9GFV0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s32480g PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.2e-124
Identity = 218/277 (78.70%), Postives = 233/277 (84.12%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MA+ ISSFWGPVTS EWCEKNYVYSSYIAEFFNTVS IP ++LALIGL+NALRQRFEKRF
Sbjct: 1   MAEAISSFWGPVTSAEWCEKNYVYSSYIAEFFNTVSIIPGILLALIGLINALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLHISNMILAIGSMLYHATLQR+                       QQQGDETPMVWEM
Sbjct: 61  SVLHISNMILAIGSMLYHATLQRM-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLY YILYSPDWHYRS MPTFLFLYGAAFA+ HA++RF  GFKVHY ILCLLC+PRMYKY
Sbjct: 121 LLYFYILYSPDWHYRSVMPTFLFLYGAAFAIFHALVRFEIGFKVHYVILCLLCVPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YI+T D  AKRLAKLYLATI  G++CWLFDRL+C NIS WYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIYTKDASAKRLAKLYLATITTGSLCWLFDRLFCNNISQWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMF RAQQL WNPKV HF+G FPYVKIQKPK+Q
Sbjct: 241 ANTFLMFWRAQQLGWNPKVAHFMGFFPYVKIQKPKTQ 254

[Close]

BLAST of Lsi05G018500 vs. TAIR10
Match: AT4G22330.1 (AT4G22330.1 Alkaline phytoceramidase (aPHC))

HSP 1 Score: 418.3 bits (1074), Expect = 3.7e-117
Identity = 202/278 (72.66%), Postives = 224/278 (80.58%), Query Frame = 1


Query: 1   MADTISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKR 60
           MAD ISSFWGPVTS  E CE NY YSSYIAEF+NT+SN+P ++LALIGLVNALRQRFEKR
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKR 60

Query: 61  FSVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWE 120
           FS+LHISNMILAIGSMLYHATLQ V                       QQQ DETPMVWE
Sbjct: 61  FSILHISNMILAIGSMLYHATLQHV-----------------------QQQSDETPMVWE 120

Query: 121 MLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYK 180
           +LLY+YILYSPDWHYRSTMPTFLFLYGAAFA+ HA +RFG GFKVHY ILCLLCIPRMYK
Sbjct: 121 ILLYMYILYSPDWHYRSTMPTFLFLYGAAFAIVHAYLRFGIGFKVHYVILCLLCIPRMYK 180

Query: 181 YYIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSY 240
           YYIHT D  AKR+AK Y+ATI +G++CW  DR++C+ IS W  NPQGHALWHV M FNSY
Sbjct: 181 YYIHTEDTAAKRIAKWYVATILVGSICWFCDRVFCKTISQWPVNPQGHALWHVFMSFNSY 240

Query: 241 FANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
            ANTFLMFCRAQQ  WNPKV +FLG+ PYVKI+KPK+Q
Sbjct: 241 CANTFLMFCRAQQRGWNPKVKYFLGVLPYVKIEKPKTQ 255

[Close]

BLAST of Lsi05G018500 vs. NCBI nr
Match: gi|659075822|ref|XP_008438349.1| (PREDICTED: alkaline ceramidase 3-like [Cucumis melo])

HSP 1 Score: 511.9 bits (1317), Expect = 6.9e-142
Identity = 249/277 (89.89%), Postives = 251/277 (90.61%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLHISNM+LAIGSMLYHATLQRV                       QQQGDETPMVWEM
Sbjct: 61  SVLHISNMVLAIGSMLYHATLQRV-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY
Sbjct: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YIHTNDIHAKRLAKLYL TI LGTVCWLFDRLYCR ISSWYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIHTNDIHAKRLAKLYLMTISLGTVCWLFDRLYCRKISSWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQQLEWNP+VVHFLGLFPYVKIQKPKSQ
Sbjct: 241 ANTFLMFCRAQQLEWNPRVVHFLGLFPYVKIQKPKSQ 254

[Close]

BLAST of Lsi05G018500 vs. NCBI nr
Match: gi|449432406|ref|XP_004133990.1| (PREDICTED: alkaline ceramidase 3-like [Cucumis sativus])

HSP 1 Score: 511.5 bits (1316), Expect = 9.0e-142
Identity = 247/277 (89.17%), Postives = 251/277 (90.61%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLHISNM+LAIGSMLYHATLQRV                       QQQGDETPMVWEM
Sbjct: 61  SVLHISNMVLAIGSMLYHATLQRV-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY
Sbjct: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YIHTND+HAKRLAKLYL TI LGTVCWLFDRLYCR ISSWYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIHTNDVHAKRLAKLYLTTISLGTVCWLFDRLYCRKISSWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQQLEWNP+VVHFLGLFPYVK+QKPKSQ
Sbjct: 241 ANTFLMFCRAQQLEWNPRVVHFLGLFPYVKVQKPKSQ 254

[Close]

BLAST of Lsi05G018500 vs. NCBI nr
Match: gi|743904501|ref|XP_011045627.1| (PREDICTED: alkaline ceramidase 3-like [Populus euphratica])

HSP 1 Score: 458.4 bits (1178), Expect = 9.0e-126
Identity = 219/277 (79.06%), Postives = 235/277 (84.84%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MA+ ISSFWGPVTS EWCEKNYVYSSYIAEFFNTVSNIP ++LALIGL+NALRQRFEKRF
Sbjct: 1   MAEAISSFWGPVTSAEWCEKNYVYSSYIAEFFNTVSNIPGILLALIGLINALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLHISNMILAIGSMLYHATLQRV                       QQQGDETPMVWEM
Sbjct: 61  SVLHISNMILAIGSMLYHATLQRV-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLY YILYSPDWHYRS MPTFLFLYGAAFA+ HA++RF  GFKVHY ILCLLC+PRMYKY
Sbjct: 121 LLYFYILYSPDWHYRSVMPTFLFLYGAAFAIFHALVRFEIGFKVHYVILCLLCVPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YI+T D  AKRLAKLYLATI +G++CWLFDRL+C NIS WYFNP+GHALWHVLMGFNSYF
Sbjct: 181 YIYTKDASAKRLAKLYLATIFMGSLCWLFDRLFCNNISQWYFNPEGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMF RAQQL WNPKV HF+G FPYVKIQKPK+Q
Sbjct: 241 ANTFLMFWRAQQLGWNPKVAHFMGFFPYVKIQKPKTQ 254

[Close]

BLAST of Lsi05G018500 vs. NCBI nr
Match: gi|728835372|gb|KHG14815.1| (Alkaline ceramidase 3 [Gossypium arboreum])

HSP 1 Score: 458.4 bits (1178), Expect = 9.0e-126
Identity = 214/277 (77.26%), Postives = 235/277 (84.84%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           M   +SSFWGPVT+ EWCEKNYVYSSYIAEFFNT+SN+PC++LALIGL+NALRQRFEKRF
Sbjct: 1   MEGGLSSFWGPVTAAEWCEKNYVYSSYIAEFFNTISNVPCILLALIGLINALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLH+SNMILAIGSMLYH+TLQ +                       QQQGDETPMVWEM
Sbjct: 61  SVLHVSNMILAIGSMLYHSTLQHM-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLY YILYSPDWHYRSTMPTFLFLYG  FA+ HA++RFG GFKVHY ILCLLCIPRMYKY
Sbjct: 121 LLYFYILYSPDWHYRSTMPTFLFLYGVGFAIVHALLRFGIGFKVHYVILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YI+T D  AKRLAKLY+AT+ LG++CWL DRL+C+ ISSWYFNPQGHALWHVLMGFNSYF
Sbjct: 181 YIYTEDASAKRLAKLYVATLFLGSICWLADRLFCKEISSWYFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRAQQ EW+PKVVHFLG FPYVKIQKPKSQ
Sbjct: 241 ANTFLMFCRAQQREWDPKVVHFLGFFPYVKIQKPKSQ 254

[Close]

BLAST of Lsi05G018500 vs. NCBI nr
Match: gi|1009139956|ref|XP_015887388.1| (PREDICTED: alkaline ceramidase 3-like [Ziziphus jujuba])

HSP 1 Score: 458.4 bits (1178), Expect = 9.0e-126
Identity = 218/277 (78.70%), Postives = 234/277 (84.48%), Query Frame = 1


Query: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCLILALIGLVNALRQRFEKRF 60
           MAD +SSFWGPVTS +WCEKNY+YS+YIAEFFNT+SNIPC+ LA IGL+NALRQRFEKRF
Sbjct: 1   MADGLSSFWGPVTSTDWCEKNYLYSAYIAEFFNTMSNIPCIALAFIGLINALRQRFEKRF 60

Query: 61  SVLHISNMILAIGSMLYHATLQRVLACSVKSSSTIISIGLNDKFNFRQQQGDETPMVWEM 120
           SVLHISN+ILA+GSMLYHATLQRV                       QQQGDETPMVWEM
Sbjct: 61  SVLHISNIILAVGSMLYHATLQRV-----------------------QQQGDETPMVWEM 120

Query: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKY 180
           LLYIYILYSPDWHYRSTMPTFLFLYGA FAVAHA++RF  GFKVHY ILCLLCIPRMYKY
Sbjct: 121 LLYIYILYSPDWHYRSTMPTFLFLYGAVFAVAHALVRFQIGFKVHYMILCLLCIPRMYKY 180

Query: 181 YIHTNDIHAKRLAKLYLATICLGTVCWLFDRLYCRNISSWYFNPQGHALWHVLMGFNSYF 240
           YIHT D+HAK LAKLYL TI L T+CWL DR++C NIS W+FNPQGHALWHVLMGFNSYF
Sbjct: 181 YIHTKDVHAKWLAKLYLITIFLATLCWLSDRVFCNNISYWFFNPQGHALWHVLMGFNSYF 240

Query: 241 ANTFLMFCRAQQLEWNPKVVHFLGLFPYVKIQKPKSQ 278
           ANTFLMFCRA QL WNPKVVHFLGL PYVKIQKPK Q
Sbjct: 241 ANTFLMFCRAHQLGWNPKVVHFLGLLPYVKIQKPKMQ 254

The following BLAST results are available for this feature:

BLAST of Lsi05G018500 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ACER3_MOUSE	4.5e-24	26.33	Alkaline ceramidase 3 OS=Mus musculus GN=Acer3 PE=2 SV=1	[more]
ACER3_HUMAN	7.1e-22	25.90	Alkaline ceramidase 3 OS=Homo sapiens GN=ACER3 PE=1 SV=3	[more]
YDC1_YEAST	5.0e-15	26.58	Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
DCD3A_DICDI	1.2e-13	24.15	Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum GN=dcd3A PE=2 SV=...	[more]
DCD3B_DICDI	1.1e-11	24.92	Putative alkaline ceramidase dcd3B OS=Dictyostelium discoideum GN=dcd3B PE=3 SV=...	[more]

BLAST of Lsi05G018500 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9D8_CUCSA	6.3e-142	89.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G130320 PE=4 SV=1	[more]
A0A0D2UTV4_GOSRA	6.3e-126	77.26	Uncharacterized protein OS=Gossypium raimondii GN=B456_011G156200 PE=4 SV=1	[more]
A0A0B0NPT2_GOSAR	6.3e-126	77.26	Alkaline ceramidase 3 OS=Gossypium arboreum GN=F383_18020 PE=4 SV=1	[more]
U5FNC0_POPTR	9.1e-125	77.26	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s08440g PE=4 SV=1	[more]
B9GFV0_POPTR	1.2e-124	78.70	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s32480g PE=4 SV=1	[more]

BLAST of Lsi05G018500 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G22330.1	3.7e-117	72.66	Alkaline phytoceramidase (aPHC)	[more]

BLAST of Lsi05G018500 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659075822\|ref\|XP_008438349.1\|	6.9e-142	89.89	PREDICTED: alkaline ceramidase 3-like [Cucumis melo]	[more]
gi\|449432406\|ref\|XP_004133990.1\|	9.0e-142	89.17	PREDICTED: alkaline ceramidase 3-like [Cucumis sativus]	[more]
gi\|743904501\|ref\|XP_011045627.1\|	9.0e-126	79.06	PREDICTED: alkaline ceramidase 3-like [Populus euphratica]	[more]
gi\|728835372\|gb\|KHG14815.1\|	9.0e-126	77.26	Alkaline ceramidase 3 [Gossypium arboreum]	[more]
gi\|1009139956\|ref\|XP_015887388.1\|	9.0e-126	78.70	PREDICTED: alkaline ceramidase 3-like [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006672	ceramide metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Vocabulary: **INTERPRO**
Term	Definition
IPR008901	Ceramidase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006672	ceramide metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G018500.1	Lsi05G018500.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008901	Ceramidase	PFAM	PF05875	Ceramidase	coord: 8..268 score: 1.3
None	No IPR available	PANTHER	PTHR12956	ALKALINE CERAMIDASE-RELATED	coord: 1..87 score: 1.2E-166coord: 103..275 score: 1.2E
None	No IPR available	PANTHER	PTHR12956:SF28	SUBFAMILY NOT NAMED	coord: 1..87 score: 1.2E-166coord: 103..275 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G018500	Csa3G130320	Cucumber (Chinese Long) v2	culsiB209
Lsi05G018500	CSPI03G10760	Wild cucumber (PI 183967)	cpilsiB207
Lsi05G018500	Cucsa.256530	Cucumber (Gy14) v1	cgylsiB415
Lsi05G018500	CmaCh06G012360	Cucurbita maxima (Rimu)	cmalsiB762
Lsi05G018500	CmaCh14G019010	Cucurbita maxima (Rimu)	cmalsiB245
Lsi05G018500	CmoCh06G012460	Cucurbita moschata (Rifu)	cmolsiB757
Lsi05G018500	CmoCh14G019540	Cucurbita moschata (Rifu)	cmolsiB233
Lsi05G018500	Cla021412	Watermelon (97103) v1	lsiwmB365
Lsi05G018500	MELO3C006305	Melon (DHL92) v3.5.1	lsimeB378
Lsi05G018500	ClCG05G003240	Watermelon (Charleston Gray)	lsiwcgB335
Lsi05G018500	MELO3C006305.2	Melon (DHL92) v3.6.1	lsimedB396
Lsi05G018500	Cla97C05G083880	Watermelon (97103) v2	lsiwmbB333
Lsi05G018500	Bhi01G000155	Wax gourd	lsiwgoB445
Lsi05G018500	Cp4.1LG03g17920	Cucurbita pepo (Zucchini)	cpelsiB506
Lsi05G018500	Cp4.1LG08g02710	Cucurbita pepo (Zucchini)	cpelsiB714
Lsi05G018500	Carg24769	Silver-seed gourd	carlsiB156
Lsi05G018500	CsaV3_3G010870	Cucumber (Chinese Long) v3	cuclsiB228

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G018500	Cucumber (Gy14) v2	cgyblsiB114
Lsi05G018500	Cucumber (Gy14) v2	cgyblsiB194
Lsi05G018500	Cucumber (Gy14) v2	cgyblsiB196
Lsi05G018500	Silver-seed gourd	carlsiB206
Lsi05G018500	Silver-seed gourd	carlsiB878
Lsi05G018500	Silver-seed gourd	carlsiB983
Lsi05G018500	Cucumber (Chinese Long) v3	cuclsiB220
Lsi05G018500	Watermelon (97103) v2	lsiwmbB341
Lsi05G018500	Watermelon (97103) v2	lsiwmbB347
Lsi05G018500	Wax gourd	lsiwgoB421
Lsi05G018500	Wax gourd	lsiwgoB462
Lsi05G018500	Bottle gourd (USVL1VR-Ls)	lsilsiB011
Lsi05G018500	Cucurbita maxima (Rimu)	cmalsiB356
Lsi05G018500	Cucurbita maxima (Rimu)	cmalsiB487
Lsi05G018500	Cucurbita maxima (Rimu)	cmalsiB586
Lsi05G018500	Cucurbita maxima (Rimu)	cmalsiB764
Lsi05G018500	Cucurbita moschata (Rifu)	cmolsiB345
Lsi05G018500	Cucurbita moschata (Rifu)	cmolsiB471
Lsi05G018500	Cucurbita moschata (Rifu)	cmolsiB566
Lsi05G018500	Cucurbita moschata (Rifu)	cmolsiB823
Lsi05G018500	Wild cucumber (PI 183967)	cpilsiB124
Lsi05G018500	Wild cucumber (PI 183967)	cpilsiB128
Lsi05G018500	Wild cucumber (PI 183967)	cpilsiB201
Lsi05G018500	Melon (DHL92) v3.5.1	lsimeB360
Lsi05G018500	Watermelon (Charleston Gray)	lsiwcgB343
Lsi05G018500	Watermelon (Charleston Gray)	lsiwcgB349
Lsi05G018500	Watermelon (97103) v1	lsiwmB360