ClCG05G003240 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG05G003240
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	Alkaline ceramidase
Location	CG_Chr05 : 3080963 .. 3083664 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATTCGAACTCTTTCGTCTGTTTATGAATTTCTCGAGGAAGTCAAGTCATGATGGAAAAATGGAAGAATTGAATAAAGTTGCCATGGTCAGCGTGGTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTCTTTTTTTTTTTTGTTCTAAATGAAAACGTTGCGATGGTTATTATGGTAATTATGTTCGAGTTAGTTTACTCTTGACGTCTCTTATTTGCACGGTAAAGAATGTTAAGGAATGATGTATGGAAGTGGCAATTACGTTTTCTTAGCTAAGTGCGATTCTAGGTTTGGCGGTTTCTGTTATTGTAAGTAAACTACGAAACATGTAGTTGGTCTTTGATATTGTCGTTTCGGGATTGTTAACTTGACCAAGGAGTTTATGAAGATGGAGAGCCTCTTTCTGTATAAACATTTCACATCTGCGGGTTGTTTTCGTTAATGAAATTGGTCAATTGATCATTTAGTTCTAAGAATCCATGATAATTCAGACAAGTTTCCTTATCAAATACATATTTCCTTCAGATACACCAGTATTTGCTTGGGTGAAAAATGGAAGTTGTTTTTACTGTTGACTTTTATGTGTGTTATCCTTTGCACTGACTATTACTTATTAAAGAATATGATTACAGGTTTGTAAATTCTGAACCATCCCAGCAGGCAATTCAAGTGTTGTCGGGTATACTTAATTGTAGAATGGCAGATACAATATCTAGTTTCTGGGGTCCTGTCACATCTCCTGAGTGGTGTGAAAAGAATTATGTCTACTCTTCTTATATTGCGGAATTCTTCAACACGATATCAAATATTCCATGCCTCATTTTAGCACTAATTGGTCTCGTAAATGCTTTGAGACAAAGGTTTGAGAAGAGGTTTAGTGTTCTTCACATATCAAATATGATCCTTGCCATAGGAAGCATGTTGTATCATGCCACCTTGCAACGAGTGTAAGTCTTCTTCCTTTATATTGAAGAATAAAGAATGCAGATGACTATGTGGCAGTCCTGTTGTTTGGCTTGGGATTTCTTTTTAATTGTTTGAGTTGAAGTTTTATTTCTATGTAGAGGCTGTGATTTGTAAATCCAAAAGTTTTTAAGCTACTGATACAATATAGATTCCACAAGCGATCAAATTACAAAATGTTGTGGTGTGGAGTTGAGGTACAAGATATTTTGGATGTGTACAAGGAAAATATGGTGAACAATAGCTTTATCCTTGTCCATGTCATTAGATATTTCTTTTTGGAGACTTTTCAGCTAAAGGAAAGATAGGAGGAAACTGCTAAGCAAATGATAAACAAGAATTATTGGGAAACTTTCATTTGTTTTTTCAACTGCCCAACAGAAATTTGGACTATGCTGTTCGATTTGCTCTGTGCTTACATGTTGGGATGGAAAGAAGGTTGATTTAGCCATTCAGGCTTACAAGAAACTTGAAAATAATTGTATCCAGTCTGTAGAAGTTGTAGTTTTAAAGTAATGGAGTTTGTTTATGTCCATGAACTTATTATAGCTTATCCACACACACAAGAAAAAAAATAAATAAATAAAATTTAGAACGGTCTCAGTATTTCATCAATTATTGGAATTCATTCTCTCATGGATGAACTTTCAGGTTCTTCTATCACCTCTGATTTTCTATTCTTCCAATGCGTTAGGAGTTGCAACTTGTTACATTTTCTTCCCCCTCCAGCCGAGGATTGGTACATGTAATGGTTTATTTCATTAATAGATGATGTATTCATAAATGTTCTCATGAACATTAACTATAGCTAGTGGATGATAACGTATTATGCCATGAAATTTCTGAATGCTTTATACAACATTAGGCTTGCATGTTCTGTCAAGTCTTCATCCACTATAATATCTTTTGGATTGAACGATAAATTTAATTTCAGACAACAGCAAGGAGATGAAACACCAATGGTATGGGAAATGCTCCTCTACATCTACATCCTTTATTCACCAGATTGGCACTATCGGAGTACGATGCCTACGTTCTTGTTTCTATATGGAGCTGCCTTTGCTGTTGCCCATGCAATCATACGTTTCGGAGCAGGCTTTAAGGTACATTATGCAATACTATGTCTTCTCTGCATCCCTCGTATGTACAAGTACTATATTCACACGAACGACATACACGCGAAGCGGCTTGCAAAGTTGTACTTGACAACCATCTCTCTTGGGACCGTTTGCTGGCTTTTTGATCGCTTGTACTGCAGGAACATCTCTAGCTGGTATTTCAACCCCCAGGGTCATGCATTATGGCATGTATTAATGGGATTCAATTCTTATTTCGCAAATACATTTTTAATGTTTTGTCGTGCTCAACAACTAGAGTGGAACCCGAGAGTTGTCCACTTTTTGGGGTTGTTTCCATACGTGAAGATTCAAAAACCAAAGAGCCAGTGACCGAAGGGTGTGTCTTGGGAAGGAAAAAGAAAAACGACATTCTTTTTTTTTTTTTCTTTTTTTAATATCTTGAAATGGGTTCTTGGTTTTGGCTTAGTTATCATCAATAGGACTAGACATTCTTTTACCCCTCCTTGCCTGTCTTTCTTCCTTCTCATATGGATGTAAATCATACTCAGAATGTCTGTGCCATTGATTTAGATTGGGTTTCTTCTCCTGCAGGTGTAAACTTGTGTTCTATCTTCTTCTTCTTCTTCTTCTTCTCCTCTTTTTGGCAGGTTACTAA

mRNA sequence

ATGGAGATTCGAACTCTTTCGTCTGTTTATGAATTTCTCGAGGAAGTCAAGTTTGTAAATTCTGAACCATCCCAGCAGGCAATTCAAGTGTTGTCGGGTATACTTAATTGTAGAATGGCAGATACAATATCTAGTTTCTGGGGTCCTGTCACATCTCCTGAGTGGTGTGAAAAGAATTATGTCTACTCTTCTTATATTGCGGAATTCTTCAACACGATATCAAATATTCCATGCCTCATTTTAGCACTAATTGGTCTCGTAAATGCTTTGAGACAAAGGTTTGAGAAGAGGTTTAGTGTTCTTCACATATCAAATATGATCCTTGCCATAGGAAGCATGTTGTATCATGCCACCTTGCAACGAGTGAGTTGCAACTTGTTACATTTTCTTCCCCCTCCAGCCGAGGATTGGCTTGCATGTTCTGTCAAGTCTTCATCCACTATAATATCTTTTGGATTGAACGATAAATTTAATTTCAGACAACAGCAAGGAGATGAAACACCAATGGTATGGGAAATGCTCCTCTACATCTACATCCTTTATTCACCAGATTGGCACTATCGGAGTACGATGCCTACGTTCTTGTTTCTATATGGAGCTGCCTTTGCTGTTGCCCATGCAATCATACGTTTCGGAGCAGGCTTTAAGGTACATTATGCAATACTATGTCTTCTCTGCATCCCTCGTATGTACAAGTACTATATTCACACGAACGACATACACGCGAAGCGGCTTGCAAAGTTGTACTTGACAACCATCTCTCTTGGGACCGTTTGCTGGCTTTTTGATCGCTTGTACTGCAGGAACATCTCTAGCTGGTGTAAACTTGTGTTCTATCTTCTTCTTCTTCTTCTTCTTCTCCTCTTTTTGGCAGGTTACTAA

Coding sequence (CDS)

ATGGAGATTCGAACTCTTTCGTCTGTTTATGAATTTCTCGAGGAAGTCAAGTTTGTAAATTCTGAACCATCCCAGCAGGCAATTCAAGTGTTGTCGGGTATACTTAATTGTAGAATGGCAGATACAATATCTAGTTTCTGGGGTCCTGTCACATCTCCTGAGTGGTGTGAAAAGAATTATGTCTACTCTTCTTATATTGCGGAATTCTTCAACACGATATCAAATATTCCATGCCTCATTTTAGCACTAATTGGTCTCGTAAATGCTTTGAGACAAAGGTTTGAGAAGAGGTTTAGTGTTCTTCACATATCAAATATGATCCTTGCCATAGGAAGCATGTTGTATCATGCCACCTTGCAACGAGTGAGTTGCAACTTGTTACATTTTCTTCCCCCTCCAGCCGAGGATTGGCTTGCATGTTCTGTCAAGTCTTCATCCACTATAATATCTTTTGGATTGAACGATAAATTTAATTTCAGACAACAGCAAGGAGATGAAACACCAATGGTATGGGAAATGCTCCTCTACATCTACATCCTTTATTCACCAGATTGGCACTATCGGAGTACGATGCCTACGTTCTTGTTTCTATATGGAGCTGCCTTTGCTGTTGCCCATGCAATCATACGTTTCGGAGCAGGCTTTAAGGTACATTATGCAATACTATGTCTTCTCTGCATCCCTCGTATGTACAAGTACTATATTCACACGAACGACATACACGCGAAGCGGCTTGCAAAGTTGTACTTGACAACCATCTCTCTTGGGACCGTTTGCTGGCTTTTTGATCGCTTGTACTGCAGGAACATCTCTAGCTGGTGTAAACTTGTGTTCTATCTTCTTCTTCTTCTTCTTCTTCTCCTCTTTTTGGCAGGTTACTAA

Protein sequence

MEIRTLSSVYEFLEEVKFVNSEPSQQAIQVLSGILNCRMADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRFSVLHISNMILAIGSMLYHATLQRVSCNLLHFLPPPAEDWLACSVKSSSTIISFGLNDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSWCKLVFYLLLLLLLLLFLAGY

Homology

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BLAST of ClCG05G003240 vs. Swiss-Prot
Match: ACER3_MOUSE (Alkaline ceramidase 3 OS=Mus musculus GN=Acer3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 8.4e-13
Identity = 34/87 (39.08%), Postives = 54/87 (62.07%), Query Frame = 1


Query: 46  FWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRFSVLHIS 105
           +WGP TS  +WCE+NYV + ++AEF+NT+SN+  +I  + G +  +R R EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMIIPPIFGAIQGIRDRLEKRYIAAYLA 69

Query: 106 NMILAIGSMLYHATLQRVSCNLLHFLP 132
             ++ +GS  +H TL +    LL  LP
Sbjct: 70  LTVVGMGSWCFHMTL-KYEMQLLDELP 95

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BLAST of ClCG05G003240 vs. Swiss-Prot
Match: YDC1_YEAST (Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YDC1 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.2e-12
Identity = 74/259 (28.57%), Postives = 116/259 (44.79%), Query Frame = 1


Query: 43  ISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRFSVL 102
           I  +WG  TS  +WCE+NYV S YIAE+ NTI+N   L+ A     +A R + E R+ ++
Sbjct: 12  IEGYWGKPTSLIDWCEENYVVSPYIAEWSNTITNSIFLMTAFYSTYSAWRNKLETRYILI 71

Query: 103 HISNMILAIGSMLYHATLQRVSCNLLHFLPPPAEDWLACSVKSSSTIISFGLNDKFNFRQ 162
            +   ++ IGS L+H TLQ     LL  LP      L  ++  S +I +          +
Sbjct: 72  GMGFSLVGIGSWLFHMTLQ-YRYQLLDELP-----MLYATIIPSWSIFAETQEILIKDEK 131

Query: 163 QQGDETPMVWEMLLYIY--ILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHY 222
           ++ + +  +  ++ +I   I+    W Y                    +++  A F+V Y
Sbjct: 132 KRKESSFRIQMVISFIMCGIVTILTWIY-------------------VVVQKPAIFQVLY 191

Query: 223 AILCLLCIPRM-YKYYIHTNDIHAKRLAKLYLTTIS------LGTVCWLFDRLYCRNISS 282
            IL LL +    +  Y H +D  AK+   L++T +       +G +CW  D   C   S 
Sbjct: 192 GILTLLVVVLSGWLTYYHVHDSFAKK--NLFITMVMGMIPFVIGFICWQLDIHLC---SF 240

Query: 283 WCKL-VFYLLLLLLLLLFL 291
           W  +   YL L L +LL L
Sbjct: 252 WIYIRRTYLALPLGVLLEL 240

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BLAST of ClCG05G003240 vs. Swiss-Prot
Match: ACER3_HUMAN (Alkaline ceramidase 3 OS=Homo sapiens GN=ACER3 PE=1 SV=3)

HSP 1 Score: 72.4 bits (176), Expect = 9.2e-12
Identity = 35/87 (40.23%), Postives = 53/87 (60.92%), Query Frame = 1


Query: 46  FWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRFSVLHIS 105
           +WGP TS  +WCE+NY  + YIAEF+NT+SN+  +I  + G V ++R   EKR+   +++
Sbjct: 10  YWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEKRYIASYLA 69

Query: 106 NMILAIGSMLYHATLQRVSCNLLHFLP 132
             ++ +GS  +H TL +    LL  LP
Sbjct: 70  LTVVGMGSWCFHMTL-KYEMQLLDELP 95

[Close]

BLAST of ClCG05G003240 vs. Swiss-Prot
Match: YPC1_YEAST (Alkaline ceramidase YPC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YPC1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.7e-10
Identity = 30/80 (37.50%), Postives = 48/80 (60.00%), Query Frame = 1


Query: 42  TISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRFSV 101
           ++   WG  TS  +WCE+NYV S YIAE+ NT++N   ++ A+    +A + + EKRF +
Sbjct: 12  SVPGVWGETTSTIDWCEENYVVSPYIAEWSNTLTNSVFILSAIYTTYSAYKNKLEKRFLL 71

Query: 102 LHISNMILAIGSMLYHATLQ 121
           +     ++ +GS L+H TL+
Sbjct: 72  IGFGYGLVGVGSWLFHMTLK 91

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BLAST of ClCG05G003240 vs. Swiss-Prot
Match: DCD3A_DICDI (Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum GN=dcd3A PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.2e-06
Identity = 30/87 (34.48%), Postives = 44/87 (50.57%), Query Frame = 1


Query: 49  PVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGL---------------VNALRQR 108
           P  S +WCE NY YS YIAEF+NT S++   +  + G+               +  L+Q 
Sbjct: 13  PTASIDWCELNYTYSPYIAEFYNTFSSLIISLFGIYGIWIMMPNFGTGVEKEHIKILKQL 72

Query: 109 FEKRFSVL-HISNMILAIGSMLYHATL 120
             +   +L +IS +++ +GS  YHATL
Sbjct: 73  DVRNKVILSYISLIVVGVGSAFYHATL 99

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BLAST of ClCG05G003240 vs. TrEMBL
Match: A0A0A0L9D8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G130320 PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-80
Identity = 160/240 (66.67%), Postives = 173/240 (72.08%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNT+SNIPCL   ++ L+           
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCL---ILALIG---------- 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG---L 158
                          L +A  QR     ++LH                S+ +++ G    
Sbjct: 61  ---------------LVNALRQRFEKRFSVLHI---------------SNMVLAIGSMLY 120

Query: 159 NDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 218
           +      QQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA
Sbjct: 121 HATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 180

Query: 219 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           GFKVHYAILCLLCIPRMYKYYIHTND+HAKRLAKLYLTTISLGTVCWLFDRLYCR ISSW
Sbjct: 181 GFKVHYAILCLLCIPRMYKYYIHTNDVHAKRLAKLYLTTISLGTVCWLFDRLYCRKISSW 197

[Close]

BLAST of ClCG05G003240 vs. TrEMBL
Match: M7ZT45_TRIUA (Alkaline ceramidase 3 OS=Triticum urartu GN=TRIUR3_07446 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.0e-78
Identity = 151/237 (63.71%), Postives = 176/237 (74.26%), Query Frame = 1


Query: 39  MADT-ISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEK 98
           MAD+ ++SFWGPVTS  E CE+NY  SSYIAEF+NT+SN PC++LALIGLVNALRQRFEK
Sbjct: 5   MADSMVASFWGPVTSTTELCEENYARSSYIAEFYNTLSNAPCILLALIGLVNALRQRFEK 64

Query: 99  RFSVLHISNMILAIGSMLYHATLQRVSCNLLHFLPPPAEDWLACSVKSSSTIISFGLNDK 158
           RFSVLHISNMIL+IGS+++HATLQ VS     F  P      A  +              
Sbjct: 65  RFSVLHISNMILSIGSIIFHATLQHVSYRRSLFATPVGPSKNALGL-------------- 124

Query: 159 FNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFK 218
                QQ DETPMVWE+LLY+Y+LYSPDWHYRSTMPTFL LYGAAFAV H  +RF   FK
Sbjct: 125 -----QQSDETPMVWEILLYLYVLYSPDWHYRSTMPTFLVLYGAAFAVVHFFVRFQVVFK 184

Query: 219 VHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           +HY  LCLLCIPRMYKYYI T D+ AKRLAKL++ TI L TVCWL DR++C+ +S W
Sbjct: 185 LHYIGLCLLCIPRMYKYYIQTKDLAAKRLAKLWVLTIFLATVCWLVDRIFCKKLSLW 222

[Close]

BLAST of ClCG05G003240 vs. TrEMBL
Match: U5FNC0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s08440g PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.8e-70
Identity = 140/240 (58.33%), Postives = 160/240 (66.67%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           MA+ ISSFWGPVTS EWCEKNYVYSSYIAEFFNTISNIP ++LAL GL+           
Sbjct: 1   MAEGISSFWGPVTSAEWCEKNYVYSSYIAEFFNTISNIPGILLALAGLI----------- 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG---L 158
                            +A  QR     ++LH                S+ I++ G    
Sbjct: 61  -----------------NAFRQRFEKRFSILHI---------------SNMILAIGSMLY 120

Query: 159 NDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 218
           +      QQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLF YGAAFA+ HA++R   
Sbjct: 121 HATLQHMQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFFYGAAFAIVHALVRLRM 180

Query: 219 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           GFKVHY ILCLLC+PRMYKYYI+T D  AKRLAKLYL TI +G++CWLFDRL+C NIS W
Sbjct: 181 GFKVHYVILCLLCVPRMYKYYIYTKDASAKRLAKLYLATIFMGSLCWLFDRLFCNNISRW 197

[Close]

BLAST of ClCG05G003240 vs. TrEMBL
Match: V4RJG7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005697mg PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.6e-69
Identity = 138/240 (57.50%), Postives = 163/240 (67.92%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           MAD +SSFWGPVTS +WCEKNYVYSSYIAEF NT+SN+PC                    
Sbjct: 1   MADGLSSFWGPVTSTDWCEKNYVYSSYIAEFLNTLSNVPC-------------------- 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG---L 158
                  ++LA+  ++ +A  QR     ++LH                S+ I++ G    
Sbjct: 61  -------ILLALFGLI-NALRQRFEKRFSVLHI---------------SNMILAIGSMLY 120

Query: 159 NDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 218
           +      QQQGDETPMVWEMLLYIYILYSPDWHY+STMPTFLFLYGAAFAVAHA+ RFG 
Sbjct: 121 HATLQHMQQQGDETPMVWEMLLYIYILYSPDWHYKSTMPTFLFLYGAAFAVAHALFRFGI 180

Query: 219 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           GFKVHYA+LCLLCIPRMYKYYIHT D+ AKRLAKLYL TI++G++CWL DRL+C  IS W
Sbjct: 181 GFKVHYAVLCLLCIPRMYKYYIHTTDVSAKRLAKLYLATITVGSLCWLCDRLFCEKISLW 197

[Close]

BLAST of ClCG05G003240 vs. TrEMBL
Match: A0A067DYF0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025339mg PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.4e-69
Identity = 138/240 (57.50%), Postives = 163/240 (67.92%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           MAD +SSFWGPVTS +WCEKNYVYSSYIAEF NT+SN+PC                    
Sbjct: 1   MADGLSSFWGPVTSTDWCEKNYVYSSYIAEFLNTLSNVPC-------------------- 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG---L 158
                  ++LA+  ++ +A  QR     ++LH                S+ I++ G    
Sbjct: 61  -------ILLALFGLI-NALRQRFEKRFSVLHI---------------SNMILAIGSMLY 120

Query: 159 NDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 218
           +      QQQGDETPMVWEMLLYIYILYSPDWHY+STMPTFLFLYGAAFAVAHA+ RFG 
Sbjct: 121 HATLQHMQQQGDETPMVWEMLLYIYILYSPDWHYKSTMPTFLFLYGAAFAVAHALFRFGI 180

Query: 219 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           GFKVHYA+LCLLCIPRMYKYYIHT D+ AKRLAKLYL TI++G++CWL DRL+C  IS W
Sbjct: 181 GFKVHYAVLCLLCIPRMYKYYIHTTDVPAKRLAKLYLATITVGSLCWLCDRLFCEKISLW 197

[Close]

BLAST of ClCG05G003240 vs. TAIR10
Match: AT4G22330.1 (AT4G22330.1 Alkaline phytoceramidase (aPHC))

HSP 1 Score: 252.7 bits (644), Expect = 2.8e-67
Identity = 133/241 (55.19%), Postives = 154/241 (63.90%), Query Frame = 1


Query: 39  MADTISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKR 98
           MAD ISSFWGPVTS  E CE NY YSSYIAEF+NTISN+P ++LALIGLV          
Sbjct: 1   MADGISSFWGPVTSTIECCEMNYAYSSYIAEFYNTISNVPGILLALIGLV---------- 60

Query: 99  FSVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG--- 158
                             +A  QR     ++LH                S+ I++ G   
Sbjct: 61  ------------------NALRQRFEKRFSILHI---------------SNMILAIGSML 120

Query: 159 LNDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFG 218
            +      QQQ DETPMVWE+LLY+YILYSPDWHYRSTMPTFLFLYGAAFA+ HA +RFG
Sbjct: 121 YHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFLFLYGAAFAIVHAYLRFG 180

Query: 219 AGFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISS 274
            GFKVHY ILCLLCIPRMYKYYIHT D  AKR+AK Y+ TI +G++CW  DR++C+ IS 
Sbjct: 181 IGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILVGSICWFCDRVFCKTISQ 198

[Close]

BLAST of ClCG05G003240 vs. NCBI nr
Match: gi|697136924|ref|XP_009622547.1| (PREDICTED: alkaline ceramidase 3-like isoform X1 [Nicotiana tomentosiformis])

HSP 1 Score: 327.0 bits (837), Expect = 3.3e-86
Identity = 160/236 (67.80%), Postives = 186/236 (78.81%), Query Frame = 1


Query: 39  MADTISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKR 98
           MAD ISSFWGPVTS  EWCE NYV SSYIAEFFNTISN+PC+ILALIGLVNALRQRFEKR
Sbjct: 1   MADDISSFWGPVTSTKEWCEPNYVLSSYIAEFFNTISNVPCIILALIGLVNALRQRFEKR 60

Query: 99  FSVLHISNMILAIGSMLYHATLQRVSCNLLHFLPPPAEDWLACSVKSSSTIISFGLNDKF 158
           FSVLH+SN+ILAIGSM+YHATL+++   ++H +     D +   +K              
Sbjct: 61  FSVLHMSNIILAIGSMIYHATLRQMK-KVIHII-----DMITYKMKM------------- 120

Query: 159 NFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKV 218
              QQQGDETPMVWEMLLY YILYSPDWHY+STMPTFLFLYGA FA+AH+ IRF  GF +
Sbjct: 121 ---QQQGDETPMVWEMLLYFYILYSPDWHYKSTMPTFLFLYGALFAIAHSQIRFDIGFML 180

Query: 219 HYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           HYA+LCLLCIPR YKYYIHT D  AK++AKLY+ T+ LG VCWL DRL+C++I+ W
Sbjct: 181 HYALLCLLCIPRTYKYYIHTEDKSAKQIAKLYVATLLLGAVCWLCDRLFCKDIARW 214

[Close]

BLAST of ClCG05G003240 vs. NCBI nr
Match: gi|702431644|ref|XP_010069019.1| (PREDICTED: uncharacterized protein LOC104456021 isoform X2 [Eucalyptus grandis])

HSP 1 Score: 321.2 bits (822), Expect = 1.8e-84
Identity = 167/289 (57.79%), Postives = 198/289 (68.51%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           M D +SSFWGPVTS +WCEKNYV+SSY+AE FNT+SNIP ++LALIGLVN+L Q FEKRF
Sbjct: 1   MEDGLSSFWGPVTSIDWCEKNYVHSSYLAELFNTVSNIPGILLALIGLVNSLSQHFEKRF 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVSCNLLHFLPPPA-------EDW--LACSVKSSSTII 158
           SVLHISN+ILAIGSMLYHATLQ+V        P  A       + W  L  S +SS  + 
Sbjct: 61  SVLHISNIILAIGSMLYHATLQQVCIQTDGSDPAKAVHSSRSFQQWGELQPSHQSSLRVA 120

Query: 159 SF-----------------------GLNDK---------FNF-------------RQQQG 218
           ++                       GL++          +NF             R  QG
Sbjct: 121 TYNRHKQEKLSHLAMVLIAALKHMEGLDETRRICAVCCLWNFQLSLHLGKNENLLRTHQG 180

Query: 219 DETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFKVHYAILCL 274
           DETPMVWE+LLYIYIL+SPDWHYRSTMPTFLFLYG AFA+ H+++RFG GFK+HYAILCL
Sbjct: 181 DETPMVWEILLYIYILHSPDWHYRSTMPTFLFLYGVAFAILHSLVRFGIGFKIHYAILCL 240

[Close]

BLAST of ClCG05G003240 vs. NCBI nr
Match: gi|449432406|ref|XP_004133990.1| (PREDICTED: alkaline ceramidase 3-like [Cucumis sativus])

HSP 1 Score: 308.1 bits (788), Expect = 1.6e-80
Identity = 160/240 (66.67%), Postives = 173/240 (72.08%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNT+SNIPCL   ++ L+           
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCL---ILALIG---------- 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG---L 158
                          L +A  QR     ++LH                S+ +++ G    
Sbjct: 61  ---------------LVNALRQRFEKRFSVLHI---------------SNMVLAIGSMLY 120

Query: 159 NDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 218
           +      QQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA
Sbjct: 121 HATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 180

Query: 219 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           GFKVHYAILCLLCIPRMYKYYIHTND+HAKRLAKLYLTTISLGTVCWLFDRLYCR ISSW
Sbjct: 181 GFKVHYAILCLLCIPRMYKYYIHTNDVHAKRLAKLYLTTISLGTVCWLFDRLYCRKISSW 197

[Close]

BLAST of ClCG05G003240 vs. NCBI nr
Match: gi|659075822|ref|XP_008438349.1| (PREDICTED: alkaline ceramidase 3-like [Cucumis melo])

HSP 1 Score: 306.2 bits (783), Expect = 6.1e-80
Identity = 160/240 (66.67%), Postives = 172/240 (71.67%), Query Frame = 1


Query: 39  MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEKRF 98
           MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNT+SNIPCL   ++ L+           
Sbjct: 1   MADTISSFWGPVTSPEWCEKNYVYSSYIAEFFNTVSNIPCL---ILALIG---------- 60

Query: 99  SVLHISNMILAIGSMLYHATLQRVS--CNLLHFLPPPAEDWLACSVKSSSTIISFG---L 158
                          L +A  QR     ++LH                S+ +++ G    
Sbjct: 61  ---------------LVNALRQRFEKRFSVLHI---------------SNMVLAIGSMLY 120

Query: 159 NDKFNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 218
           +      QQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA
Sbjct: 121 HATLQRVQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGA 180

Query: 219 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYL TISLGTVCWLFDRLYCR ISSW
Sbjct: 181 GFKVHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLMTISLGTVCWLFDRLYCRKISSW 197

[Close]

BLAST of ClCG05G003240 vs. NCBI nr
Match: gi|474344147|gb|EMS62801.1| (Alkaline ceramidase 3 [Triticum urartu])

HSP 1 Score: 301.6 bits (771), Expect = 1.5e-78
Identity = 151/237 (63.71%), Postives = 176/237 (74.26%), Query Frame = 1


Query: 39  MADT-ISSFWGPVTSP-EWCEKNYVYSSYIAEFFNTISNIPCLILALIGLVNALRQRFEK 98
           MAD+ ++SFWGPVTS  E CE+NY  SSYIAEF+NT+SN PC++LALIGLVNALRQRFEK
Sbjct: 5   MADSMVASFWGPVTSTTELCEENYARSSYIAEFYNTLSNAPCILLALIGLVNALRQRFEK 64

Query: 99  RFSVLHISNMILAIGSMLYHATLQRVSCNLLHFLPPPAEDWLACSVKSSSTIISFGLNDK 158
           RFSVLHISNMIL+IGS+++HATLQ VS     F  P      A  +              
Sbjct: 65  RFSVLHISNMILSIGSIIFHATLQHVSYRRSLFATPVGPSKNALGL-------------- 124

Query: 159 FNFRQQQGDETPMVWEMLLYIYILYSPDWHYRSTMPTFLFLYGAAFAVAHAIIRFGAGFK 218
                QQ DETPMVWE+LLY+Y+LYSPDWHYRSTMPTFL LYGAAFAV H  +RF   FK
Sbjct: 125 -----QQSDETPMVWEILLYLYVLYSPDWHYRSTMPTFLVLYGAAFAVVHFFVRFQVVFK 184

Query: 219 VHYAILCLLCIPRMYKYYIHTNDIHAKRLAKLYLTTISLGTVCWLFDRLYCRNISSW 274
           +HY  LCLLCIPRMYKYYI T D+ AKRLAKL++ TI L TVCWL DR++C+ +S W
Sbjct: 185 LHYIGLCLLCIPRMYKYYIQTKDLAAKRLAKLWVLTIFLATVCWLVDRIFCKKLSLW 222

The following BLAST results are available for this feature:

BLAST of ClCG05G003240 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ACER3_MOUSE	8.4e-13	39.08	Alkaline ceramidase 3 OS=Mus musculus GN=Acer3 PE=2 SV=1	[more]
YDC1_YEAST	4.2e-12	28.57	Alkaline ceramidase YDC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
ACER3_HUMAN	9.2e-12	40.23	Alkaline ceramidase 3 OS=Homo sapiens GN=ACER3 PE=1 SV=3	[more]
YPC1_YEAST	8.7e-10	37.50	Alkaline ceramidase YPC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
DCD3A_DICDI	1.2e-06	34.48	Putative alkaline ceramidase dcd3A OS=Dictyostelium discoideum GN=dcd3A PE=2 SV=...	[more]

BLAST of ClCG05G003240 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9D8_CUCSA	1.1e-80	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G130320 PE=4 SV=1	[more]
M7ZT45_TRIUA	1.0e-78	63.71	Alkaline ceramidase 3 OS=Triticum urartu GN=TRIUR3_07446 PE=4 SV=1	[more]
U5FNC0_POPTR	6.8e-70	58.33	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s08440g PE=4 SV=1	[more]
V4RJG7_9ROSI	2.6e-69	57.50	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005697mg PE=4 SV=1	[more]
A0A067DYF0_CITSI	3.4e-69	57.50	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025339mg PE=4 SV=1	[more]

BLAST of ClCG05G003240 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G22330.1	2.8e-67	55.19	Alkaline phytoceramidase (aPHC)	[more]

BLAST of ClCG05G003240 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|697136924\|ref\|XP_009622547.1\|	3.3e-86	67.80	PREDICTED: alkaline ceramidase 3-like isoform X1 [Nicotiana tomentosiformis]	[more]
gi\|702431644\|ref\|XP_010069019.1\|	1.8e-84	57.79	PREDICTED: uncharacterized protein LOC104456021 isoform X2 [Eucalyptus grandis]	[more]
gi\|449432406\|ref\|XP_004133990.1\|	1.6e-80	66.67	PREDICTED: alkaline ceramidase 3-like [Cucumis sativus]	[more]
gi\|659075822\|ref\|XP_008438349.1\|	6.1e-80	66.67	PREDICTED: alkaline ceramidase 3-like [Cucumis melo]	[more]
gi\|474344147\|gb\|EMS62801.1\|	1.5e-78	63.71	Alkaline ceramidase 3 [Triticum urartu]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008901	Ceramidase

Vocabulary: **Molecular Function**
Term	Definition
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Vocabulary: **Biological Process**
Term	Definition
GO:0006672	ceramide metabolic process

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006672	ceramide metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG05G003240.1	ClCG05G003240.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008901	Ceramidase	PFAM	PF05875	Ceramidase	coord: 46..277 score: 2.9
None	No IPR available	PANTHER	PTHR12956	ALKALINE CERAMIDASE-RELATED	coord: 155..273 score: 2.5E-126coord: 1..124 score: 2.5E
None	No IPR available	PANTHER	PTHR12956:SF28	SUBFAMILY NOT NAMED	coord: 155..273 score: 2.5E-126coord: 1..124 score: 2.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
ClCG05G003240	Csa3G130320	Cucumber (Chinese Long) v2	cuwcgB222
ClCG05G003240	MELO3C006305	Melon (DHL92) v3.5.1	mewcgB427
ClCG05G003240	Cla021412	Watermelon (97103) v1	wcgwmB286
ClCG05G003240	Cla97C05G083880	Watermelon (97103) v2	wcgwmbB229
ClCG05G003240	Bhi01G000155	Wax gourd	wcgwgoB428
ClCG05G003240	CSPI03G10760	Wild cucumber (PI 183967)	cpiwcgB226
ClCG05G003240	Cucsa.256530	Cucumber (Gy14) v1	cgywcgB451
ClCG05G003240	CmaCh14G019010	Cucurbita maxima (Rimu)	cmawcgB233
ClCG05G003240	CmaCh06G012360	Cucurbita maxima (Rimu)	cmawcgB743
ClCG05G003240	CmoCh14G019540	Cucurbita moschata (Rifu)	cmowcgB219
ClCG05G003240	CmoCh06G012460	Cucurbita moschata (Rifu)	cmowcgB743
ClCG05G003240	Lsi05G018500	Bottle gourd (USVL1VR-Ls)	lsiwcgB335
ClCG05G003240	Cp4.1LG03g17920	Cucurbita pepo (Zucchini)	cpewcgB559
ClCG05G003240	Cp4.1LG08g02710	Cucurbita pepo (Zucchini)	cpewcgB787
ClCG05G003240	MELO3C006305.2	Melon (DHL92) v3.6.1	medwcgB416
ClCG05G003240	Carg24769	Silver-seed gourd	carwcgB0166
ClCG05G003240	CsaV3_3G010870	Cucumber (Chinese Long) v3	cucwcgB225

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG05G003240	Watermelon (Charleston Gray)	wcgwcgB160
ClCG05G003240	Watermelon (Charleston Gray)	wcgwcgB164
ClCG05G003240	Cucurbita maxima (Rimu)	cmawcgB319
ClCG05G003240	Cucurbita maxima (Rimu)	cmawcgB737
ClCG05G003240	Cucurbita moschata (Rifu)	cmowcgB315
ClCG05G003240	Cucurbita moschata (Rifu)	cmowcgB792
ClCG05G003240	Wild cucumber (PI 183967)	cpiwcgB149
ClCG05G003240	Wild cucumber (PI 183967)	cpiwcgB247
ClCG05G003240	Cucumber (Chinese Long) v2	cuwcgB145
ClCG05G003240	Melon (DHL92) v3.5.1	mewcgB385
ClCG05G003240	Watermelon (97103) v1	wcgwmB282
ClCG05G003240	Watermelon (97103) v1	wcgwmB311
ClCG05G003240	Cucurbita pepo (Zucchini)	cpewcgB138
ClCG05G003240	Cucurbita pepo (Zucchini)	cpewcgB293
ClCG05G003240	Bottle gourd (USVL1VR-Ls)	lsiwcgB016
ClCG05G003240	Cucumber (Gy14) v2	cgybwcgB135
ClCG05G003240	Cucumber (Gy14) v2	cgybwcgB203
ClCG05G003240	Cucumber (Gy14) v2	cgybwcgB222
ClCG05G003240	Melon (DHL92) v3.6.1	medwcgB376
ClCG05G003240	Silver-seed gourd	carwcgB0220
ClCG05G003240	Silver-seed gourd	carwcgB0757
ClCG05G003240	Silver-seed gourd	carwcgB0935
ClCG05G003240	Silver-seed gourd	carwcgB1045
ClCG05G003240	Cucumber (Chinese Long) v3	cucwcgB154
ClCG05G003240	Cucumber (Chinese Long) v3	cucwcgB246
ClCG05G003240	Watermelon (97103) v2	wcgwmbB237
ClCG05G003240	Watermelon (97103) v2	wcgwmbB242
ClCG05G003240	Wax gourd	wcgwgoB412
ClCG05G003240	Wax gourd	wcgwgoB447